AT5G62710
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009488
Physical Location & Seq
Forward (+)
25187221 .. 25190722
3502 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62710.1

Sequence Viewer

Length: 1815 bp
ATGGGTATCTCGAATTGGGTTTTCTCTGTAATTTCTGTAGCTACCCTTTTTGTCTCCTGCTCCTTTGCTCTCACTCTTGATGGATTTGCTCTATTGGAATTGAAGAGTGGATTTAATGATACGAGGAACTCACTAGAGAACTGGAAAGATTCAGATGAGTCTCCTTGTTCTTGGACTGGTGTCTCCTGTAATCCTCAAGACCAAAGAGTTGTTTCTATAAACTTACCATATATGCAATTAGGAGGGATAATATCTCCTAGCATTGGGAAGCTTAGTCGATTGCAGAGACTGGCACTTCATCAGAATAGCTTACATGGGAACATTCCTAATGAAATCACCAACTGCACTGAGCTAAGAGCTATGTACTTGAGGGCGAATTTTCTCCAAGGCGGGATTCCACCGGATTTAGGCAACCTTACATTTCTTACTATATTGGATCTATCAAGCAATACACTCAAGGGTGCTATTCCTTCTTCAATTAGTCGATTGACGCGGTTACGCTCCTTGAACTTGTCAACCAACTTTTTCTCTGGTGAAATACCGGATATTGGAGTTCTCAGCAGATTCGGGGTCGAAACTTTCACTGGTAATTTGGATCTCTGTGGCCGGCAAATTCGCAAGCCATGTAGATCATCAATGGGTTTCCCTGTTGTTCTTCCTCATGCAGAAAGTGCTGATGAATCAGATTCTCCAAAGCGATCATCACGCTTGATTAAAGGAATCTTGATCGGCGCAATGTCTACAATGGCTCTTGCATTCATCGTGATCTTTGTTTTCCTATGGATTTGGATGCTCTCAAAGAAGGAAAGAAAAGTAAAGAAGTACACAGAAGTCAAGAAACAAAAGGATCCATCCGAAACAAGTAAAAAGCTGATTACTTTCCATGGTGATCTTCCATACTCTTCAACTGAGCTGATTGAGAAGCTAGAGTCTCTTGACGAGGAAGACATTGTGGGTTCGGGAGGATTTGGCACGGTTTATCGAATGGTTATGAACGATCTTGGAACCTTTGCGGTTAAAAAGATAGATAGGAGTCGACAAGGATCAGACCGAGTTTTTGAGCGAGAAGTAGAGATTTTAGGTAGTGTCAAACACATCAATCTAGTGAACCTACGTGGATACTGCCGTTTACCATCTTCAAGACTTCTCATCTATGATTATCTAACCTTAGGAAGCTTAGACGATCTTCTTCACGAACGAGCTCAAGAAGACGGTTTGTTGAACTGGAATGCTCGGTTGAAAATTGCGCTAGGTTCCGCGAGGGGTCTTGCTTATCTACACCATGATTGTAGTCCCAAAATTGTTCACCGTGACATAAAATCGAGCAATATTCTACTCAATGATAAACTAGAACCTCGAGTCTCGGACTTTGGTCTTGCAAAGCTTCTTGTTGACGAAGATGCTCATGTTACCACCGTGGTAGCTGGCACCTTTGGCTATCTTGCTCCAGAGTATCTGCAAAATGGGAGAGCGACGGAGAAGTCTGATGTGTACAGCTTTGGAGTTCTTCTCCTTGAGCTCGTTACCGGAAAAAGACCAACAGACCCGATATTCGTTAAAAGAGGCTTGAACGTCGTCGGATGGATGAACACTGTGTTGAAAGAGAATCGATTAGAGGATGTAATAGACAAGAGATGCACCGATGTCGACGAAGAGTCTGTTGAGGCATTGCTCGAGATAGCTGAGAGATGTACAGATGCTAACCCGGAGAACAGGCCGGCTATGAACCAGGTGGCTCAGTTGCTTGAGCAAGAAGTCATGTCACCTTCTTCTGGTATCGATTACTACGATGATTCTCATTCTGATTACTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase

Protein Analysis

604

Amino Acids

67.3

Weight (kDa)

5.78

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 64 1.5e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 86 - 173 9.8e-07 Leucine-rich repeat region
Pkinase PF00069 314 - 580 7.1e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 316 - 581 7.8e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1480
AccB1I GGYRCC 1 cut(s) 1427
AccI GTMKAC 3 cut(s) 740, 1036, 1647
AccII CGCG 2 cut(s) 493, 1259
AciI CCGC 4 cut(s) 390, 493, 1013, 1257
AclWI GGATC 5 cut(s) 444, 603, 842, 855, 1051
AcoI YGGCCR 1 cut(s) 604
AcsI RAATTY 2 cut(s) 376, 612
AfaI GTAC 4 cut(s) 365, 824, 1493, 1693
AfiI CCNNNNNNNGG 4 cut(s) 263, 407, 548, 1772
AgsI TTSAA 9 cut(s) 103, 477, 508, 906, 1140, 1222, 1240, 1570, 1600
AhdI GACNNNNNGTC 1 cut(s) 1654
AjnI CCWGG 1 cut(s) 1728
AjuI GAANNNNNNNTTGG 2 cut(s) 378, 410
Alw21I GWGCWC 2 cut(s) 1204, 1521
Alw26I GTCTC 6 cut(s) 58, 165, 187, 280, 936, 1366
AlwI GGATC 5 cut(s) 444, 603, 842, 855, 1051
AlwNI CAGNNNCTG 1 cut(s) 289
Ama87I CYCGRG 2 cut(s) 1356, 1673
AoxI GGCC 2 cut(s) 604, 1715
ApoI RAATTY 2 cut(s) 376, 612
ArsI GACNNNNNNTTYG 4 cut(s) 1040, 1072, 1535, 1567
AspLEI GCGC 2 cut(s) 734, 1249
AsuC2I CCSGG 1 cut(s) 1706
AsuHPI GGTGA 5 cut(s) 328, 545, 899, 1298, 1755
AvaI CYCGRG 2 cut(s) 1356, 1673
AxyI CCTNAGG 1 cut(s) 1168
BaeI ACNNNNGTAYC 2 cut(s) 1111, 1144
BamHI GGATCC 1 cut(s) 847
BanI GGYRCC 1 cut(s) 1427
BanII GRGCYC 2 cut(s) 1204, 1521
BbsI GAAGAC 2 cut(s) 951, 1215
Bbv12I GWGCWC 2 cut(s) 1204, 1521
BccI CCATC 4 cut(s) 74, 859, 1141, 1575
BceAI ACGGC 1 cut(s) 1110
BcgI CGANNNNNNTGC 2 cut(s) 1627, 1661
BciT130I CCWGG 1 cut(s) 1730
BciVI GTATCC 1 cut(s) 1112
BcnI CCSGG 1 cut(s) 1706
BcoDI GTCTC 6 cut(s) 58, 165, 187, 280, 936, 1366
BfaI CTAG 6 cut(s) 134, 258, 926, 1103, 1250, 1349
BfmI CTRYAG 1 cut(s) 36
BfuI GTATCC 1 cut(s) 1112
Bme1390I CCNGG 2 cut(s) 1706, 1730
BmeRI GACNNNNNGTC 1 cut(s) 1654
BmeT110I CYCGRG 2 cut(s) 1356, 1673
BmiI GGNNCC 4 cut(s) 849, 1006, 1255, 1429
BmrFI CCNGG 2 cut(s) 1706, 1730
BmsI GCATC 4 cut(s) 780, 1390, 1625, 1687
BoxI GACNNNNGTC 2 cut(s) 179, 1371
BpiI GAAGAC 2 cut(s) 951, 1215
BplI GAGNNNNNCTC 2 cut(s) 1494, 1526
BpmI CTGGAG 1 cut(s) 1431
BpuEI CTTGAG 6 cut(s) 180, 388, 440, 1188, 1535, 1766
BpuMI CCSGG 1 cut(s) 1706
Bsa29I ATCGAT 2 cut(s) 1609, 1779
BsaAI YACGTR 1 cut(s) 1115
BsaJI CCNNGG 3 cut(s) 385, 883, 1416
BsaWI WCCGGW 3 cut(s) 400, 541, 1526
Bsc4I CCNNNNNNNGG 4 cut(s) 263, 407, 548, 1772
Bse118I RCCGGY 2 cut(s) 606, 1717
Bse1I ACTGG 5 cut(s) 146, 181, 294, 589, 1229
Bse21I CCTNAGG 1 cut(s) 1168
Bse3DI GCAATG 2 cut(s) 741, 1667
BseBI CCWGG 1 cut(s) 1730
BseCI ATCGAT 2 cut(s) 1609, 1779
BseDI CCNNGG 3 cut(s) 385, 883, 1416
BseGI GGATG 5 cut(s) 795, 851, 1586, 1590, 1624
BseLI CCNNNNNNNGG 4 cut(s) 263, 407, 548, 1772
BseMI GCAATG 2 cut(s) 741, 1667
BseMII CTCAG 5 cut(s) 339, 571, 900, 1674, 1751
BseNI ACTGG 5 cut(s) 146, 181, 294, 589, 1229
BsgI GTGCAG 1 cut(s) 328
Bsh1236I CGCG 2 cut(s) 493, 1259
BshFI GGCC 2 cut(s) 606, 1717
BshNI GGYRCC 1 cut(s) 1427
BshVI ATCGAT 2 cut(s) 1609, 1779
BsiHKAI GWGCWC 2 cut(s) 1204, 1521
BsiHKCI CYCGRG 2 cut(s) 1356, 1673
BsiSI CCGG 6 cut(s) 401, 542, 607, 1527, 1706, 1718
BslFI GGGAC 1 cut(s) 1278
BslI CCNNNNNNNGG 4 cut(s) 263, 407, 548, 1772
BsmAI GTCTC 6 cut(s) 58, 165, 187, 280, 936, 1366
BsmFI GGGAC 1 cut(s) 1278
BsmI GAATGC 2 cut(s) 755, 1234
BsnI GGCC 2 cut(s) 606, 1717
BsoBI CYCGRG 2 cut(s) 1356, 1673
Bsp1286I GDGCHC 2 cut(s) 1204, 1521
Bsp1407I TGTACA 2 cut(s) 1491, 1691
Bsp19I CCATGG 1 cut(s) 883
BspACI CCGC 4 cut(s) 390, 493, 1013, 1257
BspANI GGCC 2 cut(s) 606, 1717
BspCNI CTCAG 5 cut(s) 340, 570, 901, 1675, 1750
BspDI ATCGAT 2 cut(s) 1609, 1779
BspFNI CGCG 2 cut(s) 493, 1259
BspLI GGNNCC 4 cut(s) 849, 1006, 1255, 1429
BspPI GGATC 5 cut(s) 444, 603, 842, 855, 1051
BspT107I GGYRCC 1 cut(s) 1427
BsrDI GCAATG 2 cut(s) 741, 1667
BsrFI RCCGGY 2 cut(s) 606, 1717
BsrGI TGTACA 2 cut(s) 1491, 1691
BsrI ACTGG 5 cut(s) 146, 181, 294, 589, 1229
BssAI RCCGGY 2 cut(s) 606, 1717
BssECI CCNNGG 3 cut(s) 385, 883, 1416
BssT1I CCWWGG 2 cut(s) 385, 883
Bst2UI CCWGG 1 cut(s) 1730
Bst4CI ACNGT 6 cut(s) 976, 1214, 1310, 1417, 1594, 1811
Bst6I CTCTTC 3 cut(s) 98, 907, 1647
BstAPI GCANNNNNTGC 1 cut(s) 671
BstAUI TGTACA 2 cut(s) 1491, 1691
BstBAI YACGTR 1 cut(s) 1115
BstC8I GCNNGC 4 cut(s) 608, 620, 1426, 1719
BstDEI CTNAG 9 cut(s) 272, 348, 353, 557, 909, 1168, 1177, 1683, 1737
BstDSI CCRYGG 2 cut(s) 883, 1416
BstF5I GGATG 5 cut(s) 795, 851, 1586, 1590, 1624
BstFNI CGCG 2 cut(s) 493, 1259
BstHHI GCGC 2 cut(s) 734, 1249
BstMAI GTCTC 6 cut(s) 58, 165, 187, 280, 936, 1366
BstMWI GCNNNNNNNGC 2 cut(s) 671, 1434
BstNI CCWGG 1 cut(s) 1730
BstPAI GACNNNNGTC 2 cut(s) 179, 1371
BstSCI CCNGG 2 cut(s) 1704, 1728
BstSFI CTRYAG 1 cut(s) 36
BstUI CGCG 2 cut(s) 493, 1259
BstV2I GAAGAC 2 cut(s) 951, 1215
BstX2I RGATCY 3 cut(s) 436, 595, 847
BstYI RGATCY 3 cut(s) 436, 595, 847
Bsu15I ATCGAT 2 cut(s) 1609, 1779
Bsu36I CCTNAGG 1 cut(s) 1168
BsuI GTATCC 1 cut(s) 1112
BsuRI GGCC 2 cut(s) 606, 1717
BsuTUI ATCGAT 2 cut(s) 1609, 1779
BtgI CCRYGG 2 cut(s) 883, 1416
BtsCI GGATG 5 cut(s) 795, 851, 1586, 1590, 1624
BtsIMutI CAGTG 3 cut(s) 345, 582, 1590
Cac8I GCNNGC 4 cut(s) 608, 620, 1426, 1719
CaiI CAGNNNCTG 1 cut(s) 289
CfoI GCGC 2 cut(s) 734, 1249
Cfr10I RCCGGY 2 cut(s) 606, 1717
ClaI ATCGAT 2 cut(s) 1609, 1779
CseI GACGC 1 cut(s) 499
CsiI ACCWGGT 1 cut(s) 1728
Csp6I GTAC 4 cut(s) 364, 823, 1492, 1692
CviAII CATG 7 cut(s) 314, 624, 662, 884, 1283, 1406, 1759
CviQI GTAC 4 cut(s) 364, 823, 1492, 1692
DdeI CTNAG 9 cut(s) 272, 348, 353, 557, 909, 1168, 1177, 1683, 1737
DrdI GACNNNNNNGTC 1 cut(s) 1480
DriI GACNNNNNGTC 1 cut(s) 1654
DseDI GACNNNNNNGTC 1 cut(s) 1480
EaeI YGGCCR 1 cut(s) 604
Eam1104I CTCTTC 3 cut(s) 98, 907, 1647
Eam1105I GACNNNNNGTC 1 cut(s) 1654
EarI CTCTTC 3 cut(s) 98, 907, 1647
Ecl136II GAGCTC 2 cut(s) 1202, 1519
Eco130I CCWWGG 2 cut(s) 385, 883
Eco24I GRGCYC 2 cut(s) 1204, 1521
Eco53kI GAGCTC 2 cut(s) 1202, 1519
Eco81I CCTNAGG 1 cut(s) 1168
Eco88I CYCGRG 2 cut(s) 1356, 1673
EcoICRI GAGCTC 2 cut(s) 1202, 1519
EcoRII CCWGG 1 cut(s) 1728
EcoT14I CCWWGG 2 cut(s) 385, 883
EcoT38I GRGCYC 2 cut(s) 1204, 1521
ErhI CCWWGG 2 cut(s) 385, 883
FaeI CATG 7 cut(s) 317, 627, 665, 887, 1286, 1409, 1762
FaqI GGGAC 1 cut(s) 1278
FatI CATG 7 cut(s) 313, 623, 661, 883, 1282, 1405, 1758
FauI CCCGC 1 cut(s) 383
FblI GTMKAC 3 cut(s) 740, 1036, 1647
FokI GGATG 5 cut(s) 802, 838, 1593, 1597, 1631
FriOI GRGCYC 2 cut(s) 1204, 1521
FspBI CTAG 6 cut(s) 134, 258, 926, 1103, 1250, 1349
GlaI GCGC 2 cut(s) 733, 1248
GsuI CTGGAG 1 cut(s) 1431
HaeIII GGCC 2 cut(s) 606, 1717
HapII CCGG 6 cut(s) 401, 542, 607, 1527, 1706, 1718
HgaI GACGC 1 cut(s) 499
HhaI GCGC 2 cut(s) 734, 1249
Hin1II CATG 7 cut(s) 317, 627, 665, 887, 1286, 1409, 1762
Hin6I GCGC 2 cut(s) 732, 1247
HinP1I GCGC 2 cut(s) 732, 1247
HincII GTYRAC 4 cut(s) 516, 1037, 1393, 1648
HindII GTYRAC 4 cut(s) 516, 1037, 1393, 1648
HindIII AAGCTT 3 cut(s) 269, 1174, 1382
HpaII CCGG 6 cut(s) 401, 542, 607, 1527, 1706, 1718
HphI GGTGA 5 cut(s) 328, 545, 899, 1298, 1755
Hpy188I TCNGA 9 cut(s) 154, 303, 685, 856, 1048, 1366, 1486, 1580, 1804
Hpy99I CGWCG 4 cut(s) 1477, 1577, 1580, 1652
HpyAV CCTTC 3 cut(s) 480, 796, 1776
HpyCH4III ACNGT 6 cut(s) 976, 1214, 1310, 1417, 1594, 1811
HpyCH4IV ACGT 2 cut(s) 1114, 1572
HpyCH4V TGCA 8 cut(s) 235, 283, 345, 665, 755, 1379, 1459, 1638
HpyF10VI GCNNNNNNNGC 2 cut(s) 671, 1434
HpyF3I CTNAG 9 cut(s) 272, 348, 353, 557, 909, 1168, 1177, 1683, 1737
HpySE526I ACGT 2 cut(s) 1114, 1572
Hsp92II CATG 7 cut(s) 317, 627, 665, 887, 1286, 1409, 1762
HspAI GCGC 2 cut(s) 732, 1247
KroI GCCGGC 2 cut(s) 606, 1717
KroNI GCCGGC 2 cut(s) 608, 1719
LmnI GCTCC 3 cut(s) 65, 506, 1450
LweI GCATC 4 cut(s) 780, 1390, 1625, 1687
MabI ACCWGGT 1 cut(s) 1728
MaeI CTAG 6 cut(s) 134, 258, 926, 1103, 1250, 1349
MaeII ACGT 2 cut(s) 1114, 1572
MaeIII GTNAC 5 cut(s) 495, 1310, 1408, 1522, 1761
MflI RGATCY 3 cut(s) 436, 595, 847
MhlI GDGCHC 2 cut(s) 1204, 1521
MlyI GAGTC 5 cut(s) 167, 938, 1042, 1368, 1664
MmeI TCCRAC 1 cut(s) 1558
MroNI GCCGGC 2 cut(s) 606, 1717
MseI TTAA 4 cut(s) 114, 714, 1017, 1557
MspI CCGG 6 cut(s) 401, 542, 607, 1527, 1706, 1718
MspR9I CCNGG 2 cut(s) 1706, 1730
Mva1269I GAATGC 2 cut(s) 755, 1234
MvaI CCWGG 1 cut(s) 1730
MvnI CGCG 2 cut(s) 493, 1259
MwoI GCNNNNNNNGC 2 cut(s) 671, 1434
NaeI GCCGGC 2 cut(s) 608, 1719
NciI CCSGG 1 cut(s) 1706
NcoI CCATGG 1 cut(s) 883
NgoMIV GCCGGC 2 cut(s) 606, 1717
NlaIII CATG 7 cut(s) 317, 627, 665, 887, 1286, 1409, 1762
NlaIV GGNNCC 4 cut(s) 849, 1006, 1255, 1429
NmuCI GTSAC 2 cut(s) 1310, 1761
PaeR7I CTCGAG 2 cut(s) 1356, 1673
PcsI WCGNNNNNNNCGW 1 cut(s) 1785
PctI GAATGC 2 cut(s) 755, 1234
PdiI GCCGGC 2 cut(s) 608, 1719
PfeI GAWTC 8 cut(s) 149, 394, 564, 680, 686, 720, 1606, 1793
PleI GAGTC 5 cut(s) 166, 937, 1041, 1367, 1663
PpsI GAGTC 5 cut(s) 166, 937, 1041, 1367, 1663
Ppu21I YACGTR 1 cut(s) 1115
PshAI GACNNNNGTC 2 cut(s) 179, 1371
Psp124BI GAGCTC 2 cut(s) 1204, 1521
Psp6I CCWGG 1 cut(s) 1728
PspGI CCWGG 1 cut(s) 1728
PspN4I GGNNCC 4 cut(s) 849, 1006, 1255, 1429
PspXI VCTCGAGB 1 cut(s) 1356
PstNI CAGNNNCTG 1 cut(s) 289
PsuI RGATCY 3 cut(s) 436, 595, 847
RsaI GTAC 4 cut(s) 365, 824, 1493, 1693
RsaNI GTAC 4 cut(s) 364, 823, 1492, 1692
SacI GAGCTC 2 cut(s) 1204, 1521
SalI GTCGAC 2 cut(s) 1035, 1646
SaqAI TTAA 4 cut(s) 114, 714, 1017, 1557
SchI GAGTC 5 cut(s) 167, 938, 1042, 1368, 1664
ScrFI CCNGG 2 cut(s) 1706, 1730
SduI GDGCHC 2 cut(s) 1204, 1521
SexAI ACCWGGT 1 cut(s) 1728
SfaNI GCATC 4 cut(s) 780, 1390, 1625, 1687
SfcI CTRYAG 1 cut(s) 36
Sfr274I CTCGAG 2 cut(s) 1356, 1673
SlaI CTCGAG 2 cut(s) 1356, 1673
SmlI CTYRAG 8 cut(s) 195, 367, 455, 1203, 1356, 1514, 1673, 1745
SmoI CTYRAG 8 cut(s) 195, 367, 455, 1203, 1356, 1514, 1673, 1745
SsiI CCGC 4 cut(s) 390, 493, 1013, 1257
SspI AATATT 1 cut(s) 1330
SspMI CTAG 6 cut(s) 134, 258, 926, 1103, 1250, 1349
SstI GAGCTC 2 cut(s) 1204, 1521
StyD4I CCNGG 2 cut(s) 1704, 1728
StyI CCWWGG 2 cut(s) 385, 883
TaaI ACNGT 6 cut(s) 976, 1214, 1310, 1417, 1594, 1811
TaiI ACGT 2 cut(s) 1117, 1575
TaqII GACCGA 1 cut(s) 1065
TatI WGTACW 4 cut(s) 363, 822, 1491, 1691
TfiI GAWTC 8 cut(s) 149, 394, 564, 680, 686, 720, 1606, 1793
Tru1I TTAA 4 cut(s) 114, 714, 1017, 1557
Tru9I TTAA 4 cut(s) 114, 714, 1017, 1557
TscAI CASTG 3 cut(s) 352, 589, 1597
TseFI GTSAC 2 cut(s) 1310, 1761
Tsp45I GTSAC 2 cut(s) 1310, 1761
TspDTI ATGAA 7 cut(s) 287, 345, 693, 748, 1007, 1601, 1739
TspGWI ACGGA 1 cut(s) 1490
TspRI CASTG 3 cut(s) 352, 589, 1597
XapI RAATTY 2 cut(s) 376, 612
XhoI CTCGAG 2 cut(s) 1356, 1673
XmiI GTMKAC 3 cut(s) 740, 1036, 1647
XspI CTAG 6 cut(s) 134, 258, 926, 1103, 1250, 1349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.