Rw7G002860

LRR receptor-like serine threonine-protein kinase FEI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009488
Physical Location & Seq
Forward (+)
2369591 .. 2373479
3889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002860.1

Sequence Viewer

Length: 1812 bp
ATGAAGAAGGGTGTTTTGTTTTGGGTCTTCTCAGTGATTGTAGCAGCAACTGTTTACTCCCATTGTTCTCTTGCTCTCAGCCAAGATGGTTTCGTACTGCTGGAAATGAAAAGCGTTTTGAATGACAGTAGGAACATGCTTAGTAACTGGCAGGATTCTGATGAATCACCTTGTAATTGGACTGGTATTACTTGCCATCCTCAGGACCAAAGAGTCAGCGCTATTAACCTACCCTATATGCAACTAGGAGGGACTATATCTCCCAGCATTGGCAAACTCAGTAGATTGCAGAGACTGGCGCTTCACCAGAACAGCTTACATGGATTCATTCCTAATGAAATTACCAGTTGCACTGAGCTTAGAGCTTTGTACTTGAGGGCTAATTATCTACAGGGAGGTATACCATCAAATATTGGAAACCTTTCTTCTCTCACCATATTGGATATATCAAGCAATTTATTAAAGGGTGCTATACCTTCATCTATTGGCCGCCTATCACGATTGCGCTCTCTGAACTTGTCCACTAACTTCTTCTCTGGGGAAATCCCAGACGTTGGAGTTCTGAGCAACTTTGGGAATAAGTCGTTTATTGGGAATCTTGATCTTTGTGGCCAACAAGTGCACAAGCCCTGTCGAACCTCACTGGGATTTCCTGCTGTATTGCCACATGCGGCGAGTGATGAAGCAGCTGTCCCTCCCAAGCGATCTTCACATTACATTAAGGGATTGCTTATTGGTGTCATGGCGACCCTGGCATTTGCACTTTTCATTCTCCTTGGCTTCCTTTGGGCTCGGTTACTATCGAAAAAGGAAAGAGTGGCCAAGAAATATACAGAAGTTACGAAGCAAGTAAATCAAGAAGCAGGCACAAAACTCATCACTTTCCATGGAGATCTGCCCTACCCTTCGTGTGAGATCATTGACAAGTTAGAGTCGCTTGATGAAGAAGATGTTGTAGGATCAGGAGGATTTGGCACTGTGTACCGAATGGTCATGAATGATTGTGGAACATTTGCTGTTAAAAGAATTGACAGAAGTCGGGAAGGATCAGATCAAGGTTTTGAGAGGGAGTTAGAGATCTTGGGTAGCATTAAGCATATAAACCTAGTTAACTTGCGAGGCTACTGCAGGCTTCCTACTTCAAAGCTTCTTATATATGATTATGTAGCTATGGGAAGCTTAGATGATTTCTTGCATGGAGATTGGCTGGAAGAGCGACCTCTGAATTGGAGTGTGCGTTTAAGAATAGCTCTCGGTTCTGCTAGAGGGATAGCATACCTGCACCATGATTGCTGTCCAAAGATAGTGCACCGTGACATAAAATCCAGCAACATTTTGCTTGATGAAAACTTGGAGCCCCATGTCTCTGACTTTGGTCTTGCAAAGCTTTTGGTTGATGAGGATGCCCATGTTACCACAGTGGTTGCTGGCACTTTTGGCTATCTTGCACCAGAGTATTTGCAGAGCGGGAGAGCGACACAAAAGTCAGATGTATATAGCTTTGGAGTTCTTTTGCTTGAGCTTGTGACTGGAAAGAGACCTACTGATCCGACATTTGTCAAGAGAGGCTTAAATGTAGTTGGTTGGATGAACACGCTATTGAGAGAGAACCGATTGAAAGATTTAGTTGACAAGCAGTGCAGAGATGCAGATCCTGAAAGTCTGGAAGCAATTCTTGAAATAGCTGCAAGGTGCACGGATGCGAGCCCAGATGATAGGCCATCAATGAATCAGGTATTGCAGTTGCTAGAGCAAGAAGTCATGTCACCTTGCCCAAGTGATTTCTACGACTCCCACTCAGATCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

603

Amino Acids

66.79

Weight (kDa)

6.12

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 65 4e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 83 - 174 3.5e-07 Leucine-rich repeat region
LRR_8 PF13855 120 - 176 1.4e-07 Leucine rich repeat
Pkinase PF00069 315 - 581 3.5e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 316 - 582 1.5e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 212, 1483
Acc36I ACCTGC 1 cut(s) 1287
AccB7I CCANNNNNTGG 1 cut(s) 554
AccBSI CCGCTC 1 cut(s) 1467
AccI GTMKAC 1 cut(s) 400
AciI CCGC 3 cut(s) 490, 671, 1467
AclWI GGATC 4 cut(s) 967, 1054, 1541, 1646
AcoI YGGCCR 3 cut(s) 487, 610, 819
AfaI GTAC 3 cut(s) 96, 371, 983
AfeI AGCGCT 1 cut(s) 220
AfiI CCNNNNNNNGG 3 cut(s) 202, 269, 554
AgsI TTSAA 4 cut(s) 121, 1143, 1618, 1679
AjnI CCWGG 1 cut(s) 750
Alw21I GWGCWC 3 cut(s) 624, 1311, 1697
Alw26I GTCTC 3 cut(s) 286, 1369, 1531
Alw44I GTGCAC 3 cut(s) 620, 1307, 1693
AlwI GGATC 4 cut(s) 967, 1054, 1541, 1646
AlwNI CAGNNNCTG 3 cut(s) 50, 295, 1655
Aor51HI AGCGCT 1 cut(s) 220
AoxI GGCC 4 cut(s) 487, 610, 819, 1718
ApaLI GTGCAC 3 cut(s) 620, 1307, 1693
ApeKI GCWGC 3 cut(s) 44, 686, 1685
Asp700I GAANNNNTTC 2 cut(s) 421, 1671
AspLEI GCGC 3 cut(s) 221, 301, 507
AspS9I GGNCC 1 cut(s) 205
AsuHPI GGTGA 4 cut(s) 159, 296, 424, 1758
AvaII GGWCC 1 cut(s) 205
AxyI CCTNAGG 1 cut(s) 201
BaeGI GKGCMC 3 cut(s) 624, 1311, 1697
BalI TGGCCA 2 cut(s) 612, 821
BanII GRGCYC 3 cut(s) 793, 1359, 1709
BbsI GAAGAC 1 cut(s) 19
Bbv12I GWGCWC 3 cut(s) 624, 1311, 1697
BbvI GCAGC 3 cut(s) 56, 698, 1672
BccI CCATC 4 cut(s) 80, 204, 412, 1729
BcgI CGANNNNNNTGC 2 cut(s) 1107, 1141
BciT130I CCWGG 1 cut(s) 752
BcoDI GTCTC 3 cut(s) 286, 1369, 1531
BfaI CTAG 4 cut(s) 245, 1106, 1263, 1748
BfmI CTRYAG 2 cut(s) 389, 1126
BfoI RGCGCY 2 cut(s) 222, 302
BfuAI ACCTGC 1 cut(s) 1287
BglII AGATCT 2 cut(s) 892, 1077
BisI GCNGC 5 cut(s) 45, 490, 672, 687, 1686
BlsI GCNGC 5 cut(s) 46, 491, 673, 688, 1687
Bme1390I CCNGG 1 cut(s) 752
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 1 cut(s) 1356
BmrFI CCNGG 1 cut(s) 752
BmrI ACTGGG 1 cut(s) 653
BmsI GCATC 3 cut(s) 1393, 1636, 1690
BmuI ACTGGG 1 cut(s) 653
BoxI GACNNNNGTC 3 cut(s) 1035, 1374, 1556
BpiI GAAGAC 1 cut(s) 19
BpuEI CTTGAG 2 cut(s) 394, 1538
BsaBI GATNNNNATC 1 cut(s) 1650
BsaI GGTCTC 1 cut(s) 1531
BsaJI CCNNGG 3 cut(s) 750, 775, 886
BsaXI ACNNNNNCTCC 4 cut(s) 244, 274, 1222, 1252
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 269, 554
Bse1I ACTGG 6 cut(s) 152, 187, 300, 345, 648, 1534
Bse21I CCTNAGG 1 cut(s) 201
Bse8I GATNNNNATC 1 cut(s) 1650
BseBI CCWGG 1 cut(s) 752
BseDI CCNNGG 3 cut(s) 750, 775, 886
BseGI GGATG 4 cut(s) 196, 1408, 1593, 1705
BseJI GATNNNNATC 1 cut(s) 1650
BseLI CCNNNNNNNGG 3 cut(s) 202, 269, 554
BseMII CTCAG 7 cut(s) 45, 91, 215, 292, 345, 554, 1812
BseNI ACTGG 6 cut(s) 152, 187, 300, 345, 648, 1534
BseSI GKGCMC 3 cut(s) 624, 1311, 1697
BseXI GCAGC 3 cut(s) 56, 698, 1672
BseYI CCCAGC 1 cut(s) 263
BsgI GTGCAG 2 cut(s) 1265, 1660
BshFI GGCC 4 cut(s) 489, 612, 821, 1720
BsiHKAI GWGCWC 3 cut(s) 624, 1311, 1697
BslFI GGGAC 2 cut(s) 265, 677
BslI CCNNNNNNNGG 3 cut(s) 202, 269, 554
BsmAI GTCTC 3 cut(s) 286, 1369, 1531
BsmFI GGGAC 2 cut(s) 265, 677
BsnI GGCC 4 cut(s) 489, 612, 821, 1720
Bso31I GGTCTC 1 cut(s) 1531
Bsp1286I GDGCHC 6 cut(s) 624, 793, 1311, 1359, 1697, 1709
Bsp19I CCATGG 1 cut(s) 886
BspACI CCGC 3 cut(s) 490, 671, 1467
BspANI GGCC 4 cut(s) 489, 612, 821, 1720
BspCNI CTCAG 7 cut(s) 44, 90, 214, 291, 346, 555, 1811
BspHI TCATGA 1 cut(s) 993
BspLI GGNNCC 1 cut(s) 1356
BspMAI CTGCAG 1 cut(s) 1130
BspMI ACCTGC 1 cut(s) 1287
BspPI GGATC 4 cut(s) 967, 1054, 1541, 1646
BspQI GCTCTTC 1 cut(s) 1206
BspTNI GGTCTC 1 cut(s) 1531
BsrBI CCGCTC 1 cut(s) 1467
BsrI ACTGG 6 cut(s) 152, 187, 300, 345, 648, 1534
BssECI CCNNGG 3 cut(s) 750, 775, 886
BssNAI GTATAC 1 cut(s) 401
BssT1I CCWWGG 2 cut(s) 775, 886
Bst1107I GTATAC 1 cut(s) 401
Bst2UI CCWGG 1 cut(s) 752
Bst4CI ACNGT 5 cut(s) 52, 128, 979, 1313, 1420
Bst6I CTCTTC 1 cut(s) 1206
BstC8I GCNNGC 4 cut(s) 865, 1130, 1429, 1705
BstDSI CCRYGG 1 cut(s) 886
BstF5I GGATG 4 cut(s) 196, 1408, 1593, 1705
BstH2I RGCGCY 2 cut(s) 222, 302
BstHHI GCGC 3 cut(s) 221, 301, 507
BstMAI GTCTC 3 cut(s) 286, 1369, 1531
BstMWI GCNNNNNNNGC 3 cut(s) 752, 1213, 1437
BstNI CCWGG 1 cut(s) 752
BstNSI RCATGY 2 cut(s) 139, 671
BstPAI GACNNNNGTC 3 cut(s) 1035, 1374, 1556
BstSCI CCNGG 1 cut(s) 750
BstSFI CTRYAG 2 cut(s) 389, 1126
BstSLI GKGCMC 3 cut(s) 624, 1311, 1697
BstV1I GCAGC 3 cut(s) 56, 698, 1672
BstV2I GAAGAC 1 cut(s) 19
BstX2I RGATCY 3 cut(s) 892, 1077, 1651
BstYI RGATCY 3 cut(s) 892, 1077, 1651
BstZ17I GTATAC 1 cut(s) 401
Bsu36I CCTNAGG 1 cut(s) 201
BsuRI GGCC 4 cut(s) 489, 612, 821, 1720
BtgI CCRYGG 1 cut(s) 886
BtsCI GGATG 4 cut(s) 196, 1408, 1593, 1705
BtsI GCAGTG 1 cut(s) 1643
BtsIMutI CAGTG 6 cut(s) 39, 351, 641, 975, 1425, 1643
BveI ACCTGC 1 cut(s) 1287
Cac8I GCNNGC 4 cut(s) 865, 1130, 1429, 1705
CaiI CAGNNNCTG 3 cut(s) 50, 295, 1655
CciI TCATGA 1 cut(s) 993
CfoI GCGC 3 cut(s) 221, 301, 507
Cfr13I GGNCC 1 cut(s) 205
Csp6I GTAC 3 cut(s) 95, 370, 982
CspCI CAANNNNNGTGG 2 cut(s) 1405, 1440
CviQI GTAC 3 cut(s) 95, 370, 982
DrdI GACNNNNNNGTC 2 cut(s) 212, 1483
DseDI GACNNNNNNGTC 2 cut(s) 212, 1483
EaeI YGGCCR 3 cut(s) 487, 610, 819
Eam1104I CTCTTC 1 cut(s) 1206
EarI CTCTTC 1 cut(s) 1206
Eco130I CCWWGG 2 cut(s) 775, 886
Eco24I GRGCYC 3 cut(s) 793, 1359, 1709
Eco31I GGTCTC 1 cut(s) 1531
Eco47I GGWCC 1 cut(s) 205
Eco47III AGCGCT 1 cut(s) 220
Eco81I CCTNAGG 1 cut(s) 201
EcoRII CCWGG 1 cut(s) 750
EcoT14I CCWWGG 2 cut(s) 775, 886
EcoT38I GRGCYC 3 cut(s) 793, 1359, 1709
ErhI CCWWGG 2 cut(s) 775, 886
FalI AAGNNNNNCTT 4 cut(s) 1553, 1585, 1659, 1691
FaqI GGGAC 2 cut(s) 265, 677
FauI CCCGC 1 cut(s) 1460
FblI GTMKAC 1 cut(s) 400
Fnu4HI GCNGC 5 cut(s) 45, 490, 672, 687, 1686
FokI GGATG 4 cut(s) 183, 1415, 1600, 1712
FriOI GRGCYC 3 cut(s) 793, 1359, 1709
Fsp4HI GCNGC 5 cut(s) 45, 490, 672, 687, 1686
FspBI CTAG 4 cut(s) 245, 1106, 1263, 1748
GlaI GCGC 3 cut(s) 220, 300, 506
GluI GCNGC 5 cut(s) 45, 490, 672, 687, 1686
GsaI CCCAGC 1 cut(s) 267
HaeII RGCGCY 2 cut(s) 222, 302
HaeIII GGCC 4 cut(s) 489, 612, 821, 1720
HhaI GCGC 3 cut(s) 221, 301, 507
Hin6I GCGC 3 cut(s) 219, 299, 505
HinP1I GCGC 3 cut(s) 219, 299, 505
HincII GTYRAC 2 cut(s) 1111, 1630
HindII GTYRAC 2 cut(s) 1111, 1630
HindIII AAGCTT 3 cut(s) 1145, 1177, 1385
HinfI GANTC 8 cut(s) 155, 164, 213, 324, 595, 932, 1729, 1790
HpaI GTTAAC 1 cut(s) 1111
HphI GGTGA 4 cut(s) 159, 296, 424, 1758
Hpy166II GTNNAC 9 cut(s) 55, 401, 522, 622, 982, 1111, 1309, 1630, 1695
Hpy188I TCNGA 9 cut(s) 160, 513, 564, 1051, 1224, 1369, 1489, 1551, 1801
Hpy8I GTNNAC 9 cut(s) 55, 401, 522, 622, 982, 1111, 1309, 1630, 1695
HpyAV CCTTC 3 cut(s) 486, 915, 1037
HpyCH4III ACNGT 5 cut(s) 52, 128, 979, 1313, 1420
HpyCH4IV ACGT 1 cut(s) 552
HpyF10VI GCNNNNNNNGC 3 cut(s) 752, 1213, 1437
HpySE526I ACGT 1 cut(s) 552
HspAI GCGC 3 cut(s) 219, 299, 505
KspAI GTTAAC 1 cut(s) 1111
LguI GCTCTTC 1 cut(s) 1206
LmnI GCTCC 1 cut(s) 1354
Lsp1109I GCAGC 3 cut(s) 56, 698, 1672
LweI GCATC 3 cut(s) 1393, 1636, 1690
MaeI CTAG 4 cut(s) 245, 1106, 1263, 1748
MaeII ACGT 1 cut(s) 552
MaeIII GTNAC 7 cut(s) 143, 795, 838, 1313, 1411, 1525, 1764
MbiI CCGCTC 1 cut(s) 1467
MboII GAAGA 8 cut(s) 16, 19, 417, 523, 699, 956, 959, 1223
MflI RGATCY 3 cut(s) 892, 1077, 1651
MhlI GDGCHC 6 cut(s) 624, 793, 1311, 1359, 1697, 1709
MlsI TGGCCA 2 cut(s) 612, 821
MluCI AATT 7 cut(s) 175, 339, 382, 454, 1026, 1225, 1671
MluNI TGGCCA 2 cut(s) 612, 821
MlyI GAGTC 3 cut(s) 222, 941, 1784
MmeI TCCRAC 3 cut(s) 535, 1565, 1574
Mox20I TGGCCA 2 cut(s) 612, 821
MroXI GAANNNNTTC 2 cut(s) 421, 1671
MscI TGGCCA 2 cut(s) 612, 821
MseI TTAA 8 cut(s) 225, 461, 720, 1020, 1092, 1110, 1241, 1571
Msp20I TGGCCA 2 cut(s) 612, 821
MspA1I CMGCKG 1 cut(s) 689
MspR9I CCNGG 1 cut(s) 752
MvaI CCWGG 1 cut(s) 752
MwoI GCNNNNNNNGC 3 cut(s) 752, 1213, 1437
NcoI CCATGG 1 cut(s) 886
NlaIV GGNNCC 1 cut(s) 1356
NmuCI GTSAC 3 cut(s) 1313, 1525, 1764
NspI RCATGY 2 cut(s) 139, 671
PagI TCATGA 1 cut(s) 993
PciSI GCTCTTC 1 cut(s) 1206
PdmI GAANNNNTTC 2 cut(s) 421, 1671
PfeI GAWTC 5 cut(s) 155, 164, 324, 595, 1729
PflMI CCANNNNNTGG 1 cut(s) 554
PkrI GCNGC 5 cut(s) 46, 491, 673, 688, 1687
PleI GAGTC 3 cut(s) 221, 940, 1784
PpsI GAGTC 3 cut(s) 221, 940, 1784
PshAI GACNNNNGTC 3 cut(s) 1035, 1374, 1556
Psp6I CCWGG 1 cut(s) 750
PspFI CCCAGC 1 cut(s) 263
PspGI CCWGG 1 cut(s) 750
PspN4I GGNNCC 1 cut(s) 1356
PspPI GGNCC 1 cut(s) 205
PstI CTGCAG 1 cut(s) 1130
PstNI CAGNNNCTG 3 cut(s) 50, 295, 1655
PsuI RGATCY 3 cut(s) 892, 1077, 1651
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 3 cut(s) 96, 371, 983
RsaNI GTAC 3 cut(s) 95, 370, 982
SapI GCTCTTC 1 cut(s) 1206
SaqAI TTAA 8 cut(s) 225, 461, 720, 1020, 1092, 1110, 1241, 1571
SatI GCNGC 5 cut(s) 45, 490, 672, 687, 1686
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 3 cut(s) 222, 941, 1784
ScrFI CCNGG 1 cut(s) 752
SduI GDGCHC 6 cut(s) 624, 793, 1311, 1359, 1697, 1709
SfaNI GCATC 3 cut(s) 1393, 1636, 1690
SfcI CTRYAG 2 cut(s) 389, 1126
SinI GGWCC 1 cut(s) 205
SmlI CTYRAG 2 cut(s) 373, 1517
SmoI CTYRAG 2 cut(s) 373, 1517
Sse9I AATT 7 cut(s) 175, 339, 382, 454, 1026, 1225, 1671
SsiI CCGC 3 cut(s) 490, 671, 1467
SspI AATATT 1 cut(s) 412
SspMI CTAG 4 cut(s) 245, 1106, 1263, 1748
StyD4I CCNGG 1 cut(s) 750
StyI CCWWGG 2 cut(s) 775, 886
TaaI ACNGT 5 cut(s) 52, 128, 979, 1313, 1420
TaiI ACGT 1 cut(s) 555
TaqI TCGA 2 cut(s) 634, 803
TasI AATT 7 cut(s) 175, 339, 382, 454, 1026, 1225, 1671
TatI WGTACW 1 cut(s) 369
TauI GCSGC 2 cut(s) 492, 674
TfiI GAWTC 5 cut(s) 155, 164, 324, 595, 1729
Tru1I TTAA 8 cut(s) 225, 461, 720, 1020, 1092, 1110, 1241, 1571
Tru9I TTAA 8 cut(s) 225, 461, 720, 1020, 1092, 1110, 1241, 1571
TscAI CASTG 6 cut(s) 39, 358, 648, 982, 1425, 1643
TseFI GTSAC 3 cut(s) 1313, 1525, 1764
TseI GCWGC 3 cut(s) 44, 686, 1685
Tsp45I GTSAC 3 cut(s) 1313, 1525, 1764
TspGWI ACGGA 1 cut(s) 1712
TspRI CASTG 6 cut(s) 39, 358, 648, 982, 1425, 1643
Van91I CCANNNNNTGG 1 cut(s) 554
VneI GTGCAC 3 cut(s) 620, 1307, 1693
VpaK11BI GGWCC 1 cut(s) 205
XceI RCATGY 2 cut(s) 139, 671
XmiI GTMKAC 1 cut(s) 400
XmnI GAANNNNTTC 2 cut(s) 421, 1671
XspI CTAG 4 cut(s) 245, 1106, 1263, 1748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.