Rroxscaffold_3G00272820

LRR receptor-like serine threonine-protein kinase FEI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009488
Physical Location & Seq
Reverse (-)
64776419 .. 64780218
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00272820.1

Sequence Viewer

Length: 1638 bp
ATGAAGAAGGGTGTTTTGTTTTGGGTCTTCTCAGTGATTGTAGCAGCAACTGTTTACTCCCATTGTTCTCTTGCTCTCAGCCAAGATGGTTTCGTACTGCTGGAAATGAAAAGCGTTTTGAATGATAGTAGGAACATGCTCAGTAACTGGCAGGATTCTGATGAATCACCTTGTAATTGGACCGGTATTACTTGCCATCCTCAGGACCAAAGAGTCAGCGCTATTAACCTACCCTATATGCAACTAGGAGGGACTATATCTCCCAGCATTGGCAAACTCAGTAGATTGCAGAGACTGGCGCTTCACCAGAACAGCTTACATGGATTCATTCCTAATGAAATTACCAGTTGCACTGAGCTTAGAGCTTTGTACTTGAGGGCTAATTATCTACAGGGAGGTATACCATCAAATATTGGAAATCTTTCTTCTCTCACCATATTGGATATATCAAGCAATTTATTAAAGGGTGCTATACCTTCATCTATTGGCCGCCTATCACGATTGCGCTCTCTGAACTTGTCCACTAACTTCTTCTCTGGGGAAATCCCAGACGTTGGAGTTCTGAGCAACTTTGGGAATAAGTCGTTTATTGGGAATCTTGATCTTTGTGGCCAACAAGTGCACAAGCCCTGTCGAACCTCACTGGGATTTCCTGCTGTATTGCCACATGCGGCGAGTGATGAAGCAGCTGGCACAAAACTCATCACTTTCCACGGAGATCTGCCCTACCCTTCGTGTGAGATCATTGACAAGTTAGAGTCGCTTGATGAAGAAGATGTTGTAGGATCAGGAGGATTTGGCACTGTGTACCGAATGGTCATGAATGATTGTGGAACATTTGCTGTTAAAAGAATTGACAGAAGTCGCGAAGGATCAGATCAAGGTTTTGAGAGGGAGTTAGAGATCTTGGGTAGCATTAAGCATATAAACCTAGTTAACTTGCGAGGCTACTGCAGGCTTCCTTCTTCCAAGCTTCTTATATATGATTATGTAGCTTTGGGAAGCTTAGATGATTTCTTGCATGGAGATGGGCTGGAAGAGCGACCTCTGAATTGGAGTGTGCGTTTAAGAATAGCTCTTGGTTCTGCTAGAGGGATAGCATACCTGCACCATGATTGCTGTCCAAAGATAGTGCACCGTGACATAAAATCCAGCAACATTTTGCTTGATGAAAACTTGGAGCCCCATGTCTCTGACTTTGGTCTTGCAAAGCTTTTGGTTGATGAGGATGCCCATGTTACCACAGTGGTTGCTGGCACTTTTGGCTATCTTGCACCAGAGTATTTGCAGAGCGGGAGAGCGACACAAAAGTCAGATGTATATAGCTTTGGAGTTCTTTTGCTTGAGCTTGTGACTGGAAAGAGACCTACTGATCCGACATTTGTCAAGAGAGGCTTAAATGTAGTTGGTTGGATGAACACGCTATTGAGAGAGAACCGATTGAAAGATTTAGTTGACAAGCGGTGCAGAGATGCAGATCCTGAAAGTCTGGAAGCAATTCTTGAAATAGCTGCAAGGTGCACGGATGCGAGCCCAGATGATAGGCCATCAATGAATCAGGTATTGCAGTTGCTAGAGCAAGAAGTCATGTCACCTTGCCCAAGTGATTTCTACGACTCCCACTCAGATCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

545

Amino Acids

60.1

Weight (kDa)

5.61

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 65 3.5e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 88 - 175 3.6e-07 Leucine-rich repeat region
LRR_8 PF13855 120 - 176 1.2e-07 Leucine rich repeat
Pkinase PF00069 257 - 523 8.9e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 258 - 524 1.2e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 212, 1309
Acc36I ACCTGC 1 cut(s) 1113
AccB7I CCANNNNNTGG 1 cut(s) 554
AccBSI CCGCTC 1 cut(s) 1293
AccI GTMKAC 1 cut(s) 400
AccII CGCG 1 cut(s) 867
AciI CCGC 4 cut(s) 490, 671, 1293, 1462
AclWI GGATC 4 cut(s) 793, 880, 1367, 1472
AcoI YGGCCR 2 cut(s) 487, 610
AfaI GTAC 3 cut(s) 96, 371, 809
AfeI AGCGCT 1 cut(s) 220
AfiI CCNNNNNNNGG 3 cut(s) 202, 269, 554
AgeI ACCGGT 1 cut(s) 182
AgsI TTSAA 3 cut(s) 121, 1444, 1505
Alw21I GWGCWC 3 cut(s) 624, 1137, 1523
Alw26I GTCTC 3 cut(s) 286, 1195, 1357
Alw44I GTGCAC 3 cut(s) 620, 1133, 1519
AlwI GGATC 4 cut(s) 793, 880, 1367, 1472
AlwNI CAGNNNCTG 4 cut(s) 50, 147, 295, 1481
Aor51HI AGCGCT 1 cut(s) 220
AoxI GGCC 3 cut(s) 487, 610, 1544
ApaLI GTGCAC 3 cut(s) 620, 1133, 1519
ApeKI GCWGC 3 cut(s) 44, 686, 1511
AsiGI ACCGGT 1 cut(s) 182
Asp700I GAANNNNTTC 2 cut(s) 421, 1497
AspLEI GCGC 3 cut(s) 221, 301, 507
AspS9I GGNCC 2 cut(s) 180, 205
AsuHPI GGTGA 4 cut(s) 159, 296, 424, 1584
AvaII GGWCC 2 cut(s) 180, 205
AxyI CCTNAGG 1 cut(s) 201
BaeGI GKGCMC 3 cut(s) 624, 1137, 1523
BalI TGGCCA 1 cut(s) 612
BanII GRGCYC 2 cut(s) 1185, 1535
BbsI GAAGAC 1 cut(s) 19
Bbv12I GWGCWC 3 cut(s) 624, 1137, 1523
BbvI GCAGC 3 cut(s) 56, 698, 1498
BccI CCATC 5 cut(s) 80, 204, 412, 1022, 1555
BcgI CGANNNNNNTGC 2 cut(s) 933, 967
BcoDI GTCTC 3 cut(s) 286, 1195, 1357
BfaI CTAG 4 cut(s) 245, 932, 1089, 1574
BfmI CTRYAG 2 cut(s) 389, 952
BfoI RGCGCY 2 cut(s) 222, 302
BfuAI ACCTGC 1 cut(s) 1113
BglII AGATCT 2 cut(s) 718, 903
BisI GCNGC 5 cut(s) 45, 490, 672, 687, 1512
BlsI GCNGC 5 cut(s) 46, 491, 673, 688, 1513
Bme18I GGWCC 2 cut(s) 180, 205
BmgT120I GGNCC 2 cut(s) 180, 205
BmiI GGNNCC 1 cut(s) 1182
BmrI ACTGGG 1 cut(s) 653
BmsI GCATC 3 cut(s) 1219, 1462, 1516
BmuI ACTGGG 1 cut(s) 653
BoxI GACNNNNGTC 3 cut(s) 861, 1200, 1382
BpiI GAAGAC 1 cut(s) 19
BpuEI CTTGAG 2 cut(s) 394, 1364
BsaBI GATNNNNATC 1 cut(s) 1476
BsaI GGTCTC 1 cut(s) 1357
BsaJI CCNNGG 1 cut(s) 712
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 4 cut(s) 244, 274, 1048, 1078
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 269, 554
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 5 cut(s) 152, 300, 345, 648, 1360
Bse21I CCTNAGG 1 cut(s) 201
Bse8I GATNNNNATC 1 cut(s) 1476
BseDI CCNNGG 1 cut(s) 712
BseGI GGATG 4 cut(s) 196, 1234, 1419, 1531
BseJI GATNNNNATC 1 cut(s) 1476
BseLI CCNNNNNNNGG 3 cut(s) 202, 269, 554
BseMII CTCAG 8 cut(s) 45, 91, 154, 215, 292, 345, 554, 1638
BseNI ACTGG 5 cut(s) 152, 300, 345, 648, 1360
BseSI GKGCMC 3 cut(s) 624, 1137, 1523
BseXI GCAGC 3 cut(s) 56, 698, 1498
BseYI CCCAGC 1 cut(s) 263
BsgI GTGCAG 2 cut(s) 1091, 1486
Bsh1236I CGCG 1 cut(s) 867
BshFI GGCC 3 cut(s) 489, 612, 1546
BshTI ACCGGT 1 cut(s) 182
BsiHKAI GWGCWC 3 cut(s) 624, 1137, 1523
BsiSI CCGG 1 cut(s) 183
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 3 cut(s) 202, 269, 554
BsmAI GTCTC 3 cut(s) 286, 1195, 1357
BsmFI GGGAC 1 cut(s) 265
BsnI GGCC 3 cut(s) 489, 612, 1546
Bso31I GGTCTC 1 cut(s) 1357
Bsp1286I GDGCHC 5 cut(s) 624, 1137, 1185, 1523, 1535
Bsp68I TCGCGA 1 cut(s) 867
BspACI CCGC 4 cut(s) 490, 671, 1293, 1462
BspANI GGCC 3 cut(s) 489, 612, 1546
BspCNI CTCAG 8 cut(s) 44, 90, 153, 214, 291, 346, 555, 1637
BspFNI CGCG 1 cut(s) 867
BspHI TCATGA 1 cut(s) 819
BspLI GGNNCC 1 cut(s) 1182
BspMAI CTGCAG 1 cut(s) 956
BspMI ACCTGC 1 cut(s) 1113
BspPI GGATC 4 cut(s) 793, 880, 1367, 1472
BspQI GCTCTTC 1 cut(s) 1032
BspTNI GGTCTC 1 cut(s) 1357
BsrBI CCGCTC 1 cut(s) 1293
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 5 cut(s) 152, 300, 345, 648, 1360
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 712
BssNAI GTATAC 1 cut(s) 401
Bst1107I GTATAC 1 cut(s) 401
Bst4CI ACNGT 4 cut(s) 52, 805, 1139, 1246
Bst6I CTCTTC 1 cut(s) 1032
BstC8I GCNNGC 4 cut(s) 691, 956, 1255, 1531
BstDSI CCRYGG 1 cut(s) 712
BstF5I GGATG 4 cut(s) 196, 1234, 1419, 1531
BstFNI CGCG 1 cut(s) 867
BstH2I RGCGCY 2 cut(s) 222, 302
BstHHI GCGC 3 cut(s) 221, 301, 507
BstMAI GTCTC 3 cut(s) 286, 1195, 1357
BstMWI GCNNNNNNNGC 2 cut(s) 1039, 1263
BstNSI RCATGY 2 cut(s) 139, 671
BstPAI GACNNNNGTC 3 cut(s) 861, 1200, 1382
BstSFI CTRYAG 2 cut(s) 389, 952
BstSLI GKGCMC 3 cut(s) 624, 1137, 1523
BstUI CGCG 1 cut(s) 867
BstV1I GCAGC 3 cut(s) 56, 698, 1498
BstV2I GAAGAC 1 cut(s) 19
BstX2I RGATCY 3 cut(s) 718, 903, 1477
BstYI RGATCY 3 cut(s) 718, 903, 1477
BstZ17I GTATAC 1 cut(s) 401
Bsu36I CCTNAGG 1 cut(s) 201
BsuRI GGCC 3 cut(s) 489, 612, 1546
BtgI CCRYGG 1 cut(s) 712
BtsCI GGATG 4 cut(s) 196, 1234, 1419, 1531
BtsIMutI CAGTG 5 cut(s) 39, 351, 641, 801, 1251
BtuMI TCGCGA 1 cut(s) 867
BveI ACCTGC 1 cut(s) 1113
Cac8I GCNNGC 4 cut(s) 691, 956, 1255, 1531
CaiI CAGNNNCTG 4 cut(s) 50, 147, 295, 1481
CciI TCATGA 1 cut(s) 819
CfoI GCGC 3 cut(s) 221, 301, 507
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 2 cut(s) 180, 205
Csp6I GTAC 3 cut(s) 95, 370, 808
CspAI ACCGGT 1 cut(s) 182
CspCI CAANNNNNGTGG 2 cut(s) 1231, 1266
CviAII CATG 9 cut(s) 136, 320, 668, 820, 1022, 1112, 1187, 1235, 1588
CviQI GTAC 3 cut(s) 95, 370, 808
DrdI GACNNNNNNGTC 2 cut(s) 212, 1309
DseDI GACNNNNNNGTC 2 cut(s) 212, 1309
EaeI YGGCCR 2 cut(s) 487, 610
Eam1104I CTCTTC 1 cut(s) 1032
EarI CTCTTC 1 cut(s) 1032
Eco24I GRGCYC 2 cut(s) 1185, 1535
Eco31I GGTCTC 1 cut(s) 1357
Eco47I GGWCC 2 cut(s) 180, 205
Eco47III AGCGCT 1 cut(s) 220
Eco81I CCTNAGG 1 cut(s) 201
EcoT38I GRGCYC 2 cut(s) 1185, 1535
FaeI CATG 9 cut(s) 139, 323, 671, 823, 1025, 1115, 1190, 1238, 1591
FalI AAGNNNNNCTT 4 cut(s) 1379, 1411, 1485, 1517
FaqI GGGAC 1 cut(s) 265
FatI CATG 9 cut(s) 135, 319, 667, 819, 1021, 1111, 1186, 1234, 1587
FauI CCCGC 1 cut(s) 1286
FblI GTMKAC 1 cut(s) 400
Fnu4HI GCNGC 5 cut(s) 45, 490, 672, 687, 1512
FokI GGATG 4 cut(s) 183, 1241, 1426, 1538
FriOI GRGCYC 2 cut(s) 1185, 1535
Fsp4HI GCNGC 5 cut(s) 45, 490, 672, 687, 1512
FspBI CTAG 4 cut(s) 245, 932, 1089, 1574
GlaI GCGC 3 cut(s) 220, 300, 506
GluI GCNGC 5 cut(s) 45, 490, 672, 687, 1512
GsaI CCCAGC 1 cut(s) 267
HaeII RGCGCY 2 cut(s) 222, 302
HaeIII GGCC 3 cut(s) 489, 612, 1546
HapII CCGG 1 cut(s) 183
HhaI GCGC 3 cut(s) 221, 301, 507
Hin1II CATG 9 cut(s) 139, 323, 671, 823, 1025, 1115, 1190, 1238, 1591
Hin6I GCGC 3 cut(s) 219, 299, 505
HinP1I GCGC 3 cut(s) 219, 299, 505
HincII GTYRAC 2 cut(s) 937, 1456
HindII GTYRAC 2 cut(s) 937, 1456
HindIII AAGCTT 3 cut(s) 971, 1003, 1211
HinfI GANTC 8 cut(s) 155, 164, 213, 324, 595, 758, 1555, 1616
HpaI GTTAAC 1 cut(s) 937
HpaII CCGG 1 cut(s) 183
HphI GGTGA 4 cut(s) 159, 296, 424, 1584
Hpy166II GTNNAC 9 cut(s) 55, 401, 522, 622, 808, 937, 1135, 1456, 1521
Hpy188I TCNGA 9 cut(s) 160, 513, 564, 877, 1050, 1195, 1315, 1377, 1627
Hpy8I GTNNAC 9 cut(s) 55, 401, 522, 622, 808, 937, 1135, 1456, 1521
HpyAV CCTTC 4 cut(s) 486, 741, 863, 972
HpyCH4III ACNGT 4 cut(s) 52, 805, 1139, 1246
HpyCH4IV ACGT 1 cut(s) 552
HpyF10VI GCNNNNNNNGC 2 cut(s) 1039, 1263
HpySE526I ACGT 1 cut(s) 552
Hsp92II CATG 9 cut(s) 139, 323, 671, 823, 1025, 1115, 1190, 1238, 1591
HspAI GCGC 3 cut(s) 219, 299, 505
KspAI GTTAAC 1 cut(s) 937
LguI GCTCTTC 1 cut(s) 1032
LmnI GCTCC 1 cut(s) 1180
Lsp1109I GCAGC 3 cut(s) 56, 698, 1498
LweI GCATC 3 cut(s) 1219, 1462, 1516
MaeI CTAG 4 cut(s) 245, 932, 1089, 1574
MaeII ACGT 1 cut(s) 552
MaeIII GTNAC 5 cut(s) 143, 1139, 1237, 1351, 1590
MbiI CCGCTC 1 cut(s) 1293
MboII GAAGA 8 cut(s) 16, 19, 417, 523, 782, 785, 957, 1049
MflI RGATCY 3 cut(s) 718, 903, 1477
MhlI GDGCHC 5 cut(s) 624, 1137, 1185, 1523, 1535
MlsI TGGCCA 1 cut(s) 612
MluCI AATT 7 cut(s) 175, 339, 382, 454, 852, 1051, 1497
MluNI TGGCCA 1 cut(s) 612
MlyI GAGTC 3 cut(s) 222, 767, 1610
MmeI TCCRAC 3 cut(s) 535, 1391, 1400
Mox20I TGGCCA 1 cut(s) 612
MroXI GAANNNNTTC 2 cut(s) 421, 1497
MscI TGGCCA 1 cut(s) 612
MseI TTAA 7 cut(s) 225, 461, 846, 918, 936, 1067, 1397
MslI CAYNNNNRTG 1 cut(s) 1026
Msp20I TGGCCA 1 cut(s) 612
MspA1I CMGCKG 1 cut(s) 689
MspI CCGG 1 cut(s) 183
MvnI CGCG 1 cut(s) 867
MwoI GCNNNNNNNGC 2 cut(s) 1039, 1263
NlaIII CATG 9 cut(s) 139, 323, 671, 823, 1025, 1115, 1190, 1238, 1591
NlaIV GGNNCC 1 cut(s) 1182
NmuCI GTSAC 3 cut(s) 1139, 1351, 1590
NruI TCGCGA 1 cut(s) 867
NspI RCATGY 2 cut(s) 139, 671
PagI TCATGA 1 cut(s) 819
PciSI GCTCTTC 1 cut(s) 1032
PdmI GAANNNNTTC 2 cut(s) 421, 1497
PfeI GAWTC 5 cut(s) 155, 164, 324, 595, 1555
PflMI CCANNNNNTGG 1 cut(s) 554
PinAI ACCGGT 1 cut(s) 182
PkrI GCNGC 5 cut(s) 46, 491, 673, 688, 1513
PleI GAGTC 3 cut(s) 221, 766, 1610
PpsI GAGTC 3 cut(s) 221, 766, 1610
PshAI GACNNNNGTC 3 cut(s) 861, 1200, 1382
PspFI CCCAGC 1 cut(s) 263
PspN4I GGNNCC 1 cut(s) 1182
PspPI GGNCC 2 cut(s) 180, 205
PstI CTGCAG 1 cut(s) 956
PstNI CAGNNNCTG 4 cut(s) 50, 147, 295, 1481
PsuI RGATCY 3 cut(s) 718, 903, 1477
PvuII CAGCTG 1 cut(s) 689
RruI TCGCGA 1 cut(s) 867
RsaI GTAC 3 cut(s) 96, 371, 809
RsaNI GTAC 3 cut(s) 95, 370, 808
RseI CAYNNNNRTG 1 cut(s) 1026
SapI GCTCTTC 1 cut(s) 1032
SaqAI TTAA 7 cut(s) 225, 461, 846, 918, 936, 1067, 1397
SatI GCNGC 5 cut(s) 45, 490, 672, 687, 1512
Sau96I GGNCC 2 cut(s) 180, 205
SchI GAGTC 3 cut(s) 222, 767, 1610
SduI GDGCHC 5 cut(s) 624, 1137, 1185, 1523, 1535
SfaNI GCATC 3 cut(s) 1219, 1462, 1516
SfcI CTRYAG 2 cut(s) 389, 952
SinI GGWCC 2 cut(s) 180, 205
SmiMI CAYNNNNRTG 1 cut(s) 1026
SmlI CTYRAG 2 cut(s) 373, 1343
SmoI CTYRAG 2 cut(s) 373, 1343
Sse9I AATT 7 cut(s) 175, 339, 382, 454, 852, 1051, 1497
SsiI CCGC 4 cut(s) 490, 671, 1293, 1462
SspI AATATT 1 cut(s) 412
SspMI CTAG 4 cut(s) 245, 932, 1089, 1574
TaaI ACNGT 4 cut(s) 52, 805, 1139, 1246
TaiI ACGT 1 cut(s) 555
TaqI TCGA 1 cut(s) 634
TasI AATT 7 cut(s) 175, 339, 382, 454, 852, 1051, 1497
TatI WGTACW 1 cut(s) 369
TauI GCSGC 2 cut(s) 492, 674
TfiI GAWTC 5 cut(s) 155, 164, 324, 595, 1555
Tru1I TTAA 7 cut(s) 225, 461, 846, 918, 936, 1067, 1397
Tru9I TTAA 7 cut(s) 225, 461, 846, 918, 936, 1067, 1397
TscAI CASTG 5 cut(s) 39, 358, 648, 808, 1251
TseFI GTSAC 3 cut(s) 1139, 1351, 1590
TseI GCWGC 3 cut(s) 44, 686, 1511
Tsp45I GTSAC 3 cut(s) 1139, 1351, 1590
TspGWI ACGGA 2 cut(s) 729, 1538
TspRI CASTG 5 cut(s) 39, 358, 648, 808, 1251
Van91I CCANNNNNTGG 1 cut(s) 554
VneI GTGCAC 3 cut(s) 620, 1133, 1519
VpaK11BI GGWCC 2 cut(s) 180, 205
XceI RCATGY 2 cut(s) 139, 671
XmiI GTMKAC 1 cut(s) 400
XmnI GAANNNNTTC 2 cut(s) 421, 1497
XspI CTAG 4 cut(s) 245, 932, 1089, 1574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.