MD14G1193700.v1.1

LRR receptor-like serine threonine-protein kinase FEI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009488
Physical Location & Seq
Forward (+)
28487746 .. 28491960
4215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1193700.v1.1.491

Sequence Viewer

Length: 1818 bp
ATGCAGAAGAAGGGTTTGGTTTGGATTTTCTCGATGATTTCAGTTGCAATCTTCTCCTCCCATTGCTCTCTTGCTCTCATCAGCCAAGATGGTTTGGCATTGTTGGAAATCAAAAGCACTTTGAATGACAGCAGAAACATTCTCAGTGACTGGCAGGATTCTGATGAATCACCCTGTAATTGGACTGGCATTACTTGCCATCCTCAGGACCTTAGAGTCAGCTCTATTAACTTGCCTTACATGCAACTGGGAGGGACTATATCTCCCAGCATTGGTAAACTCAGTAGATTACAGAGACTGGCACTTCACCAAAACAGCTTACATGGAAACATTCCTAATGAAATTACCAATTGTGCTGAGCTTAGGGCTCTGTACTTGAGGGCTAACTACCTTGAAGGAGGTATTCCATCAGATATCGGAAACCTTTCTTCTCTCACCATACTGGATTTATCAAGCAATTTATTAAGAGGTGCTATACCTTCATCTATCGGCCGCCTCTCAAAATTACACTTTTTGAACTTGTCCACCAACTTCTTAAGCGGTGAAATCCCAGACATTGGAATTCTAAGCACTTTTGGGAACGATTCGTTTGTTGGCAATCTAGATCTTTGTGGCCAACAAGTGCACAAGCCCTGTCGGACCTCATTGGGATTTCCTGCAGTGCTACCAAAGAATGCTGTAAGTGATGAAGCAGCAGTGCCCTCTAAACGATCTTCTCATTACATCAAAGGAGTGCTTATTGGTGCAATGTCTGCCTTGGGCTTTGCGTTTTTCACTCTCCTTACCTTCCTTTGGGTTCGGTTTCTTTCGAAAAAGGAAAGAGTGGCTAAAAAATACACTGAAGTTAAAAAGCAAGTAAATCAAGAATCAAGTACAAAACTCATTACTTTCCATGGTGATATGCCCTACCCTTCAAGTGAAATCATTGAAAAGCTAGAGTCGCTCGATGAAGAGGATGTTGTAGGATCAGGAGGATTTGGCACTGTATACAGAATGGTTATGAATGATTGTGGTACTTTTGCTGTTAAAAGAATTGATAGAAGTCGGGAAGGATGTGATCAAGTTTTTGAGAGGGAGTTAGAGATCTTGGGTAGCATTAAGCACATAAACCTAGTTAACCTGCGAGGCTACTGCAGGCTCCCTGTTTCAAAGCTTCTCATATATGATTATCTAGCTATGGGCAGTTTAGATGATTTCTTGCATGAGCATTGGGTGGAAGAGCGGCCTTTGAACTGGAATGCTCGTTTGAAAATTGCCCTGGGTTCTGCTCGAGGCATAGCATACCTGCACCATGACTGCTCTCCGAAGATAGTACACCGTGACATAAAATCGAGCAACATTCTTCTAAATGAAAACTTGGATCCCCATGTCTCTGACTTTGGCCTTGCAAAGCTTTTGGTGGATGAGGATGCCCATATTACGACAGTGGTGGCTGGCACCTTTGGCTATCTGGCTCCAGAGTATCTGCAAAGTGGGAGAGCCACTCAGAAGACAGATGTGTATAGCTTTGGAGTTCTATTGCTAGAGCTTGTGACCGGAAAAAGACCTACTGATCCTACGTTTGTAAAACGAGGCTTAAATGTTGTTGGTTGGATGAACACCCAATTGAGAGAAAACAGGCTGCATGAAGTAGTAGACAAAAGGTGCAAAGATGCAGATGCTGAAACTGTGGAAGCAATTCTTGAAATCGCGGCAAGGTGCACCGATGCAAACCCTGATGACCGGCCATCAATGAACCAGGTATTGCAGTTGCTAGAGCCAGAGGTCATGTCACCGTGCCCGAGCGATTTCTACGAGTCTCATTCAGATCATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

606

Amino Acids

67.25

Weight (kDa)

5.97

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 66 2.9e-14 Leucine rich repeat N-terminal domain
LRR_14 PF23598 88 - 177 4.5e-08 Leucine-rich repeat region
Pkinase PF00069 317 - 583 6.9e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 318 - 584 5e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
Acc36I ACCTGC 2 cut(s) 1128, 1293
AccB1I GGYRCC 1 cut(s) 1436
AccB7I CCANNNNNTGG 1 cut(s) 557
AccBSI CCGCTC 1 cut(s) 1222
AccI GTMKAC 2 cut(s) 987, 1635
AccII CGCG 1 cut(s) 1691
AciI CCGC 4 cut(s) 493, 540, 1222, 1691
AclWI GGATC 4 cut(s) 973, 1355, 1368, 1547
AcoI YGGCCR 3 cut(s) 490, 613, 1724
AcsI RAATTY 1 cut(s) 561
AcuI CTGAAG 1 cut(s) 861
AfaI GTAC 4 cut(s) 374, 874, 1015, 1314
AfiI CCNNNNNNNGG 5 cut(s) 180, 205, 272, 557, 792
AflII CTTAAG 1 cut(s) 535
AgsI TTSAA 9 cut(s) 124, 395, 517, 915, 929, 1149, 1231, 1249, 1685
AjnI CCWGG 2 cut(s) 1257, 1737
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AluBI AGCT 9 cut(s) 222, 318, 361, 934, 1153, 1175, 1393, 1506, 1528
AluI AGCT 9 cut(s) 222, 318, 361, 934, 1153, 1175, 1393, 1506, 1528
Alw21I GWGCWC 2 cut(s) 627, 1703
Alw26I GTCTC 3 cut(s) 289, 1375, 1803
Alw44I GTGCAC 2 cut(s) 623, 1699
AlwI GGATC 4 cut(s) 973, 1355, 1368, 1547
AlwNI CAGNNNCTG 3 cut(s) 150, 298, 1661
Ama87I CYCGRG 2 cut(s) 1269, 1780
AoxI GGCC 5 cut(s) 490, 613, 1223, 1381, 1724
ApaLI GTGCAC 2 cut(s) 623, 1699
ApeKI GCWGC 2 cut(s) 692, 1621
ApoI RAATTY 1 cut(s) 561
Asp700I GAANNNNTTC 2 cut(s) 424, 1677
AspS9I GGNCC 2 cut(s) 208, 639
AsuHPI GGTGA 6 cut(s) 162, 299, 427, 554, 908, 1764
AsuII TTCGAA 1 cut(s) 809
AvaI CYCGRG 2 cut(s) 1269, 1780
AvaII GGWCC 2 cut(s) 208, 639
AxyI CCTNAGG 1 cut(s) 204
BaeGI GKGCMC 4 cut(s) 627, 702, 1703, 1781
BalI TGGCCA 1 cut(s) 615
BamHI GGATCC 1 cut(s) 1360
BanI GGYRCC 1 cut(s) 1436
BanII GRGCYC 1 cut(s) 370
BbsI GAAGAC 1 cut(s) 1496
Bbv12I GWGCWC 2 cut(s) 627, 1703
BbvI GCAGC 2 cut(s) 704, 1608
BccI CCATC 4 cut(s) 83, 207, 415, 1735
BcgI CGANNNNNNTGC 2 cut(s) 1113, 1147
BciT130I CCWGG 2 cut(s) 1259, 1739
BclI TGATCA 1 cut(s) 1057
BcoDI GTCTC 3 cut(s) 289, 1375, 1803
BfaI CTAG 6 cut(s) 602, 935, 1112, 1172, 1523, 1754
BfmI CTRYAG 2 cut(s) 657, 1132
BfrI CTTAAG 1 cut(s) 535
BfuAI ACCTGC 2 cut(s) 1128, 1293
BglII AGATCT 2 cut(s) 604, 1083
BisI GCNGC 5 cut(s) 493, 693, 1223, 1622, 1692
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 5 cut(s) 494, 694, 1224, 1623, 1693
Bme1390I CCNGG 2 cut(s) 1259, 1739
Bme18I GGWCC 2 cut(s) 208, 639
BmeT110I CYCGRG 2 cut(s) 1269, 1780
BmgT120I GGNCC 2 cut(s) 208, 639
BmiI GGNNCC 4 cut(s) 1139, 1362, 1438, 1455
BmrFI CCNGG 2 cut(s) 1259, 1739
BmrI ACTGGG 1 cut(s) 257
BmsI GCATC 4 cut(s) 1399, 1642, 1648, 1696
BmuI ACTGGG 1 cut(s) 257
BpiI GAAGAC 1 cut(s) 1496
BplI GAGNNNNNCTC 2 cut(s) 1468, 1500
BpmI CTGGAG 1 cut(s) 1440
Bpu10I CCTNAGC 1 cut(s) 362
Bpu1102I GCTNAGC 1 cut(s) 357
Bpu14I TTCGAA 1 cut(s) 809
BpuEI CTTGAG 1 cut(s) 397
BsaJI CCNNGG 4 cut(s) 756, 892, 1257, 1258
BsaWI WCCGGW 1 cut(s) 1535
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 205, 272, 557, 792
Bse118I RCCGGY 1 cut(s) 1722
Bse1I ACTGG 6 cut(s) 155, 190, 252, 303, 447, 1238
Bse21I CCTNAGG 1 cut(s) 204
Bse3DI GCAATG 2 cut(s) 61, 753
BseBI CCWGG 2 cut(s) 1259, 1739
BseDI CCNNGG 4 cut(s) 756, 892, 1257, 1258
BseGI GGATG 6 cut(s) 199, 961, 1058, 1408, 1414, 1599
BseLI CCNNNNNNNGG 5 cut(s) 180, 205, 272, 557, 792
BseMI GCAATG 2 cut(s) 61, 753
BseMII CTCAG 5 cut(s) 157, 218, 295, 348, 1499
BseNI ACTGG 6 cut(s) 155, 190, 252, 303, 447, 1238
BseRI GAGGAG 1 cut(s) 46
BseSI GKGCMC 4 cut(s) 627, 702, 1703, 1781
BseX3I CGGCCG 1 cut(s) 490
BseXI GCAGC 2 cut(s) 704, 1608
BseYI CCCAGC 1 cut(s) 266
BsgI GTGCAG 1 cut(s) 1271
Bsh1236I CGCG 1 cut(s) 1691
Bsh1285I CGRYCG 1 cut(s) 493
BshFI GGCC 5 cut(s) 492, 615, 1225, 1383, 1726
BshNI GGYRCC 1 cut(s) 1436
BsiEI CGRYCG 1 cut(s) 493
BsiHKAI GWGCWC 2 cut(s) 627, 1703
BsiHKCI CYCGRG 2 cut(s) 1269, 1780
BsiSI CCGG 2 cut(s) 1536, 1723
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 5 cut(s) 180, 205, 272, 557, 792
BsmAI GTCTC 3 cut(s) 289, 1375, 1803
BsmFI GGGAC 1 cut(s) 268
BsmI GAATGC 2 cut(s) 679, 1243
BsnI GGCC 5 cut(s) 492, 615, 1225, 1383, 1726
BsoBI CYCGRG 2 cut(s) 1269, 1780
Bsp119I TTCGAA 1 cut(s) 809
Bsp1286I GDGCHC 5 cut(s) 370, 627, 702, 1703, 1781
Bsp143I GATC 8 cut(s) 604, 710, 965, 1057, 1083, 1360, 1552, 1807
Bsp1720I GCTNAGC 1 cut(s) 357
Bsp19I CCATGG 1 cut(s) 892
BspACI CCGC 4 cut(s) 493, 540, 1222, 1691
BspANI GGCC 5 cut(s) 492, 615, 1225, 1383, 1726
BspCNI CTCAG 5 cut(s) 156, 217, 294, 349, 1498
BspFNI CGCG 1 cut(s) 1691
BspLI GGNNCC 4 cut(s) 1139, 1362, 1438, 1455
BspMAI CTGCAG 2 cut(s) 661, 1136
BspMI ACCTGC 2 cut(s) 1128, 1293
BspPI GGATC 4 cut(s) 973, 1355, 1368, 1547
BspQI GCTCTTC 1 cut(s) 1212
BspT104I TTCGAA 1 cut(s) 809
BspT107I GGYRCC 1 cut(s) 1436
BspTI CTTAAG 1 cut(s) 535
BsrBI CCGCTC 1 cut(s) 1222
BsrDI GCAATG 2 cut(s) 61, 753
BsrFI RCCGGY 1 cut(s) 1722
BsrI ACTGG 6 cut(s) 155, 190, 252, 303, 447, 1238
BssAI RCCGGY 1 cut(s) 1722
BssECI CCNNGG 4 cut(s) 756, 892, 1257, 1258
BssMI GATC 8 cut(s) 604, 710, 965, 1057, 1083, 1360, 1552, 1807
BssNAI GTATAC 1 cut(s) 988
BssT1I CCWWGG 2 cut(s) 756, 892
Bst1107I GTATAC 1 cut(s) 988
Bst2UI CCWGG 2 cut(s) 1259, 1739
Bst4CI ACNGT 5 cut(s) 985, 1319, 1426, 1669, 1776
Bst6I CTCTTC 2 cut(s) 945, 1212
BstAFI CTTAAG 1 cut(s) 535
BstAPI GCANNNNNTGC 2 cut(s) 195, 752
BstBI TTCGAA 1 cut(s) 809
BstC8I GCNNGC 2 cut(s) 1136, 1435
BstDEI CTNAG 8 cut(s) 143, 204, 212, 281, 357, 362, 566, 1485
BstDSI CCRYGG 1 cut(s) 892
BstF5I GGATG 6 cut(s) 199, 961, 1058, 1408, 1414, 1599
BstFNI CGCG 1 cut(s) 1691
BstKTI GATC 8 cut(s) 607, 713, 968, 1060, 1086, 1363, 1555, 1810
BstMAI GTCTC 3 cut(s) 289, 1375, 1803
BstMBI GATC 8 cut(s) 604, 710, 965, 1057, 1083, 1360, 1552, 1807
BstMCI CGRYCG 1 cut(s) 493
BstMWI GCNNNNNNNGC 5 cut(s) 195, 241, 752, 940, 1443
BstNI CCWGG 2 cut(s) 1259, 1739
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 2 cut(s) 1257, 1737
BstSFI CTRYAG 2 cut(s) 657, 1132
BstSLI GKGCMC 4 cut(s) 627, 702, 1703, 1781
BstUI CGCG 1 cut(s) 1691
BstV1I GCAGC 2 cut(s) 704, 1608
BstV2I GAAGAC 1 cut(s) 1496
BstX2I RGATCY 3 cut(s) 604, 1083, 1360
BstYI RGATCY 3 cut(s) 604, 1083, 1360
BstZ17I GTATAC 1 cut(s) 988
BstZI CGGCCG 1 cut(s) 490
Bsu36I CCTNAGG 1 cut(s) 204
BsuRI GGCC 5 cut(s) 492, 615, 1225, 1383, 1726
BtgI CCRYGG 1 cut(s) 892
BtsCI GGATG 6 cut(s) 199, 961, 1058, 1408, 1414, 1599
BtsI GCAGTG 2 cut(s) 666, 702
BtsIMutI CAGTG 6 cut(s) 151, 666, 702, 837, 981, 1431
BveI ACCTGC 2 cut(s) 1128, 1293
Cac8I GCNNGC 2 cut(s) 1136, 1435
CaiI CAGNNNCTG 3 cut(s) 150, 298, 1661
Cfr10I RCCGGY 1 cut(s) 1722
Cfr13I GGNCC 2 cut(s) 208, 639
CsiI ACCWGGT 1 cut(s) 1737
Csp6I GTAC 4 cut(s) 373, 873, 1014, 1313
CviAII CATG 8 cut(s) 241, 323, 893, 1202, 1292, 1367, 1625, 1768
CviQI GTAC 4 cut(s) 373, 873, 1014, 1313
DdeI CTNAG 8 cut(s) 143, 204, 212, 281, 357, 362, 566, 1485
DpnI GATC 8 cut(s) 606, 712, 967, 1059, 1085, 1362, 1554, 1809
DpnII GATC 8 cut(s) 604, 710, 965, 1057, 1083, 1360, 1552, 1807
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 3 cut(s) 490, 613, 1724
EagI CGGCCG 1 cut(s) 490
Eam1104I CTCTTC 2 cut(s) 945, 1212
EarI CTCTTC 2 cut(s) 945, 1212
EclXI CGGCCG 1 cut(s) 490
Eco130I CCWWGG 2 cut(s) 756, 892
Eco24I GRGCYC 1 cut(s) 370
Eco32I GATATC 1 cut(s) 415
Eco47I GGWCC 2 cut(s) 208, 639
Eco52I CGGCCG 1 cut(s) 490
Eco57I CTGAAG 1 cut(s) 861
Eco81I CCTNAGG 1 cut(s) 204
Eco88I CYCGRG 2 cut(s) 1269, 1780
EcoO109I RGGNCCY 1 cut(s) 208
EcoRI GAATTC 1 cut(s) 561
EcoRII CCWGG 2 cut(s) 1257, 1737
EcoRV GATATC 1 cut(s) 415
EcoT14I CCWWGG 2 cut(s) 756, 892
EcoT38I GRGCYC 1 cut(s) 370
ErhI CCWWGG 2 cut(s) 756, 892
FaeI CATG 8 cut(s) 244, 326, 896, 1205, 1295, 1370, 1628, 1771
FalI AAGNNNNNCTT 4 cut(s) 720, 752, 1665, 1697
FaqI GGGAC 1 cut(s) 268
FatI CATG 8 cut(s) 240, 322, 892, 1201, 1291, 1366, 1624, 1767
FbaI TGATCA 1 cut(s) 1057
FblI GTMKAC 2 cut(s) 987, 1635
Fnu4HI GCNGC 5 cut(s) 493, 693, 1223, 1622, 1692
FokI GGATG 6 cut(s) 186, 968, 1065, 1415, 1421, 1606
FriOI GRGCYC 1 cut(s) 370
Fsp4HI GCNGC 5 cut(s) 493, 693, 1223, 1622, 1692
FspBI CTAG 6 cut(s) 602, 935, 1112, 1172, 1523, 1754
GluI GCNGC 5 cut(s) 493, 693, 1223, 1622, 1692
GsaI CCCAGC 1 cut(s) 270
GsuI CTGGAG 1 cut(s) 1440
HaeIII GGCC 5 cut(s) 492, 615, 1225, 1383, 1726
HapII CCGG 2 cut(s) 1536, 1723
Hin1II CATG 8 cut(s) 244, 326, 896, 1205, 1295, 1370, 1628, 1771
HincII GTYRAC 1 cut(s) 1117
HindII GTYRAC 1 cut(s) 1117
HindIII AAGCTT 2 cut(s) 1151, 1391
HinfI GANTC 7 cut(s) 158, 167, 216, 584, 866, 938, 1796
HpaI GTTAAC 1 cut(s) 1117
HpaII CCGG 2 cut(s) 1536, 1723
HphI GGTGA 6 cut(s) 162, 299, 427, 554, 908, 1764
Hpy166II GTNNAC 8 cut(s) 278, 525, 625, 988, 1117, 1315, 1636, 1701
Hpy188I TCNGA 8 cut(s) 163, 412, 419, 639, 1305, 1375, 1488, 1807
Hpy188III TCNNGA 8 cut(s) 31, 206, 602, 863, 969, 1046, 1457, 1682
Hpy8I GTNNAC 8 cut(s) 278, 525, 625, 988, 1117, 1315, 1636, 1701
HpyAV CCTTC 6 cut(s) 4, 389, 489, 796, 921, 1043
HpyCH4III ACNGT 5 cut(s) 985, 1319, 1426, 1669, 1776
HpyCH4IV ACGT 1 cut(s) 1559
HpyF10VI GCNNNNNNNGC 5 cut(s) 195, 241, 752, 940, 1443
HpyF3I CTNAG 8 cut(s) 143, 204, 212, 281, 357, 362, 566, 1485
HpySE526I ACGT 1 cut(s) 1559
Hsp92II CATG 8 cut(s) 244, 326, 896, 1205, 1295, 1370, 1628, 1771
Ksp22I TGATCA 1 cut(s) 1057
KspAI GTTAAC 1 cut(s) 1117
Kzo9I GATC 8 cut(s) 604, 710, 965, 1057, 1083, 1360, 1552, 1807
LguI GCTCTTC 1 cut(s) 1212
LmnI GCTCC 2 cut(s) 1143, 1459
Lsp1109I GCAGC 2 cut(s) 704, 1608
LweI GCATC 4 cut(s) 1399, 1642, 1648, 1696
MabI ACCWGGT 1 cut(s) 1737
MaeI CTAG 6 cut(s) 602, 935, 1112, 1172, 1523, 1754
MaeII ACGT 1 cut(s) 1559
MaeIII GTNAC 4 cut(s) 146, 1319, 1531, 1770
MalI GATC 8 cut(s) 606, 712, 967, 1059, 1085, 1362, 1554, 1809
MbiI CCGCTC 1 cut(s) 1222
MboI GATC 8 cut(s) 604, 710, 965, 1057, 1083, 1360, 1552, 1807
MboII GAAGA 9 cut(s) 19, 43, 420, 705, 962, 1229, 1318, 1334, 1501
MfeI CAATTG 2 cut(s) 349, 1604
MflI RGATCY 3 cut(s) 604, 1083, 1360
MhlI GDGCHC 5 cut(s) 370, 627, 702, 1703, 1781
MlsI TGGCCA 1 cut(s) 615
MluNI TGGCCA 1 cut(s) 615
MlyI GAGTC 3 cut(s) 225, 947, 1805
MmeI TCCRAC 3 cut(s) 84, 617, 1571
Mox20I TGGCCA 1 cut(s) 615
MroXI GAANNNNTTC 2 cut(s) 424, 1677
MscI TGGCCA 1 cut(s) 615
MseI TTAA 8 cut(s) 228, 464, 536, 846, 1026, 1098, 1116, 1577
Msp20I TGGCCA 1 cut(s) 615
MspCI CTTAAG 1 cut(s) 535
MspI CCGG 2 cut(s) 1536, 1723
MspR9I CCNGG 2 cut(s) 1259, 1739
MunI CAATTG 2 cut(s) 349, 1604
Mva1269I GAATGC 2 cut(s) 679, 1243
MvaI CCWGG 2 cut(s) 1259, 1739
MvnI CGCG 1 cut(s) 1691
MwoI GCNNNNNNNGC 5 cut(s) 195, 241, 752, 940, 1443
NcoI CCATGG 1 cut(s) 892
NdeII GATC 8 cut(s) 604, 710, 965, 1057, 1083, 1360, 1552, 1807
NlaIII CATG 8 cut(s) 244, 326, 896, 1205, 1295, 1370, 1628, 1771
NlaIV GGNNCC 4 cut(s) 1139, 1362, 1438, 1455
NmuCI GTSAC 4 cut(s) 146, 1319, 1531, 1770
NspI RCATGY 1 cut(s) 244
NspV TTCGAA 1 cut(s) 809
PaeR7I CTCGAG 1 cut(s) 1269
PasI CCCWGGG 1 cut(s) 1258
PciSI GCTCTTC 1 cut(s) 1212
PctI GAATGC 2 cut(s) 679, 1243
PdmI GAANNNNTTC 2 cut(s) 424, 1677
PfeI GAWTC 4 cut(s) 158, 167, 584, 866
PflMI CCANNNNNTGG 1 cut(s) 557
PkrI GCNGC 5 cut(s) 494, 694, 1224, 1623, 1693
PleI GAGTC 3 cut(s) 224, 946, 1804
PpsI GAGTC 3 cut(s) 224, 946, 1804
PpuMI RGGWCCY 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 208
Psp6I CCWGG 2 cut(s) 1257, 1737
PspFI CCCAGC 1 cut(s) 266
PspGI CCWGG 2 cut(s) 1257, 1737
PspN4I GGNNCC 4 cut(s) 1139, 1362, 1438, 1455
PspPI GGNCC 2 cut(s) 208, 639
PspPPI RGGWCCY 1 cut(s) 208
PspXI VCTCGAGB 1 cut(s) 1269
PstI CTGCAG 2 cut(s) 661, 1136
PstNI CAGNNNCTG 3 cut(s) 150, 298, 1661
PsuI RGATCY 3 cut(s) 604, 1083, 1360
RsaI GTAC 4 cut(s) 374, 874, 1015, 1314
RsaNI GTAC 4 cut(s) 373, 873, 1014, 1313
SapI GCTCTTC 1 cut(s) 1212
SaqAI TTAA 8 cut(s) 228, 464, 536, 846, 1026, 1098, 1116, 1577
SatI GCNGC 5 cut(s) 493, 693, 1223, 1622, 1692
Sau3AI GATC 8 cut(s) 604, 710, 965, 1057, 1083, 1360, 1552, 1807
Sau96I GGNCC 2 cut(s) 208, 639
SchI GAGTC 3 cut(s) 225, 947, 1805
ScrFI CCNGG 2 cut(s) 1259, 1739
SduI GDGCHC 5 cut(s) 370, 627, 702, 1703, 1781
SexAI ACCWGGT 1 cut(s) 1737
SfaNI GCATC 4 cut(s) 1399, 1642, 1648, 1696
SfcI CTRYAG 2 cut(s) 657, 1132
Sfr274I CTCGAG 1 cut(s) 1269
SfuI TTCGAA 1 cut(s) 809
SinI GGWCC 2 cut(s) 208, 639
SlaI CTCGAG 1 cut(s) 1269
SmlI CTYRAG 3 cut(s) 376, 535, 1269
SmoI CTYRAG 3 cut(s) 376, 535, 1269
SsiI CCGC 4 cut(s) 493, 540, 1222, 1691
SspMI CTAG 6 cut(s) 602, 935, 1112, 1172, 1523, 1754
StyD4I CCNGG 2 cut(s) 1257, 1737
StyI CCWWGG 2 cut(s) 756, 892
TaaI ACNGT 5 cut(s) 985, 1319, 1426, 1669, 1776
TaiI ACGT 1 cut(s) 1562
TaqI TCGA 5 cut(s) 32, 809, 945, 1270, 1331
TatI WGTACW 3 cut(s) 372, 872, 1312
TauI GCSGC 3 cut(s) 495, 1225, 1694
TfiI GAWTC 4 cut(s) 158, 167, 584, 866
Tru1I TTAA 8 cut(s) 228, 464, 536, 846, 1026, 1098, 1116, 1577
Tru9I TTAA 8 cut(s) 228, 464, 536, 846, 1026, 1098, 1116, 1577
TscAI CASTG 6 cut(s) 151, 666, 702, 844, 988, 1431
TseFI GTSAC 4 cut(s) 146, 1319, 1531, 1770
TseI GCWGC 2 cut(s) 692, 1621
Tsp45I GTSAC 4 cut(s) 146, 1319, 1531, 1770
TspRI CASTG 6 cut(s) 151, 666, 702, 844, 988, 1431
Van91I CCANNNNNTGG 1 cut(s) 557
Vha464I CTTAAG 1 cut(s) 535
VneI GTGCAC 2 cut(s) 623, 1699
VpaK11BI GGWCC 2 cut(s) 208, 639
XapI RAATTY 1 cut(s) 561
XbaI TCTAGA 1 cut(s) 601
XceI RCATGY 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 1269
XmiI GTMKAC 2 cut(s) 987, 1635
XmnI GAANNNNTTC 2 cut(s) 424, 1677
XspI CTAG 6 cut(s) 602, 935, 1112, 1172, 1523, 1754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.