FvH4_5g15570

LRR receptor-like serine threonine-protein kinase FEI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009488
Physical Location & Seq
Reverse (-)
8779572 .. 8783786
4215 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15570.t2

Sequence Viewer

Length: 1812 bp
ATGAAGAAGGGTGTTCTGTTTTGGGTCCTCTCAGTCATTGTAGCAGCAACTGTTTGCTCTCATTGTTCTCTTGCTCTCACCCAAGATGGTCTTATACTGCTGGAAATGAAAAGCGTTTTGAATGACAGTAGGAACATGCTCAGTAACTGGCAGGATTCTGATGAATCACCTTGTAATTGGACTGGTATTTCTTGCCATCCTCAGGACCAAAGAGTCAGCGCAATTAACCTACCCTATATGGAACTAGGAGGGACTATATCTCCCAGCATTGGCAAACTCAGTAGATTGCAGAGACTGGCGCTTCACCAGAACAGCTTACACGGATTCATTCCTAATGAAATTGCCAGATGTGCTGAGCTTAGAGCTTTGTACTTGAGGGCTAATTATCTGCAAGGAGGCATACCATCAGATATTGGAAACCTTTCTGCTCTAACCATATTGGATATATCAAGTAATTTATTAAAGGGTGCAATACCTTCATCTATCGGCCGCTTATCACGACTCCGCTCTCTGAACTTGTCCACTAACTTCTTCTCCGGGGAAATCCCAGATGTTGGAGTTCTGAGCAACTTTGGGAATAAGTCGTTTATTGGGAATCTTGATCTTTGTGGCCAACAAGTGCACAAGCCCTGTCGAACCTCACTGGGATTTCCTGCTGTGTTGCCTCATGCGGCAAGTGATGAATCAGCAGCCCCTCCCAAGCGATCTTCACATTACATTAAGGGATTGCTTATTGGTGTCATGTCCACCATGGCAGTTGCACTCTTCATTCTCCTTGGCTTCCTTTGGGCTCGGTTACTATCGAAAAAGGAAAGAGTGGCCAAGAAGTATACAGAAGTTACAAAGCAAGTGAATCAAGAAGCAGGCACAAAACTTATCACATTCCATGGAGATCTGCCCTACCCTTCATGCGAGATTATTGACAAATTAGAGTCACTTGATGAAGAGGATGTTGTAGGATCAGGAGGGTTTGGCACTGTGTACCGAATGGTCATGAATGATTGTGGAACATTTGCTGTAAAAAGAATTGACAGAAGTCGGGAAGGATCAGATCAATGTTTCGAGAGGGAGTTAGAGATCTTGGGTAGCATTAAGCATATAAATCTAGTTAACTTGCGAGGCTACTGCAGGCTCCCTACTTCAAAGCTTCTTATATATGATTATGTAGCTATGGGGAGCTTAGATGATTTCTTGCATGGAGATGGGCAGGAAGAGCGACCTCTGAATTGGAGTGTCCGTTTAAGAATAGCTCTTGGTTCTGCCAGAGGGATAGCGTACCTACACCATGATTGCTGTCCAAAGATAGTGCACCGTGACATAAAATCTAGCAACATTCTGCTTGATGAAAACTTGGAGCCCCATGTCTCGGACTTTGGTCTTGCAAAGCTTTTGGTTGATGAGGATGCCCATGTTACCACAGTGGTTGCTGGCACTTTTGGCTATCTTGCACCAGAGTATTTGCAAAGCGGGAGAGCAACACAGAAGTCAGATGTATATAGCTTTGGAGTTCTTTTGCTTGAGCTTGTGACTGGAAAGAGACCTACTGATCCAACATTTGTCCAGAAAGGCTTAAATGTAGTTGGTTGGATGAACACGCTATTGAGGGAGAACCGAATGAAAGACTTTGTTGACAAGCGGTGCAGAGATGCAGATCCCGAAAGTGTGGAAGCAATTCTTGAAATAGCTGCAAGGTGCACAGATGCAAATCCAGATGATAGGCCATCAATGAATCAGGTATTACAGTTGCTAGAGCAAGAAGTCATGTCACCTTGCCCAAGTGATTTCTACGACTCCCACTCAGATCATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

604

Amino Acids

66.64

Weight (kDa)

6.04

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 65 1.7e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 88 - 175 8.4e-07 Leucine-rich repeat region
Pkinase PF00069 315 - 516 7.7e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 316 - 516 1.4e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AccB7I CCANNNNNTGG 1 cut(s) 554
AccBSI CCGCTC 1 cut(s) 507
AccI GTMKAC 1 cut(s) 830
AciI CCGC 5 cut(s) 490, 505, 671, 1467, 1636
AclWI GGATC 4 cut(s) 967, 1054, 1541, 1646
AcoI YGGCCR 3 cut(s) 487, 610, 819
AfaI GTAC 3 cut(s) 371, 983, 1277
AfiI CCNNNNNNNGG 4 cut(s) 202, 269, 554, 1366
AgsI TTSAA 3 cut(s) 121, 1143, 1679
Alw21I GWGCWC 3 cut(s) 624, 1311, 1697
Alw26I GTCTC 3 cut(s) 286, 1369, 1531
Alw44I GTGCAC 3 cut(s) 620, 1307, 1693
AlwI GGATC 4 cut(s) 967, 1054, 1541, 1646
AlwNI CAGNNNCTG 3 cut(s) 50, 147, 295
AoxI GGCC 4 cut(s) 487, 610, 819, 1718
ApaLI GTGCAC 3 cut(s) 620, 1307, 1693
ApeKI GCWGC 3 cut(s) 44, 689, 1685
Asp700I GAANNNNTTC 2 cut(s) 421, 1671
AspLEI GCGC 2 cut(s) 221, 301
AspS9I GGNCC 2 cut(s) 25, 205
AsuC2I CCSGG 1 cut(s) 538
AsuHPI GGTGA 4 cut(s) 70, 159, 296, 1758
AvaII GGWCC 2 cut(s) 25, 205
AxyI CCTNAGG 1 cut(s) 201
BaeGI GKGCMC 3 cut(s) 624, 1311, 1697
BalI TGGCCA 2 cut(s) 612, 821
BanII GRGCYC 2 cut(s) 793, 1359
Bbv12I GWGCWC 3 cut(s) 624, 1311, 1697
BbvI GCAGC 3 cut(s) 56, 701, 1672
BccI CCATC 5 cut(s) 80, 204, 412, 1196, 1729
BcgI CGANNNNNNTGC 2 cut(s) 1107, 1141
BcnI CCSGG 1 cut(s) 538
BcoDI GTCTC 3 cut(s) 286, 1369, 1531
BfaI CTAG 4 cut(s) 245, 1106, 1326, 1748
BfmI CTRYAG 1 cut(s) 1126
BfoI RGCGCY 1 cut(s) 302
BglII AGATCT 2 cut(s) 892, 1077
BisI GCNGC 5 cut(s) 45, 490, 672, 690, 1686
BlpI GCTNAGC 1 cut(s) 354
BlsI GCNGC 5 cut(s) 46, 491, 673, 691, 1687
Bme1390I CCNGG 1 cut(s) 538
Bme18I GGWCC 2 cut(s) 25, 205
BmgT120I GGNCC 2 cut(s) 25, 205
BmiI GGNNCC 3 cut(s) 26, 1133, 1356
BmrFI CCNGG 1 cut(s) 538
BmrI ACTGGG 1 cut(s) 653
BmsI GCATC 3 cut(s) 1393, 1636, 1690
BmuI ACTGGG 1 cut(s) 653
BoxI GACNNNNGTC 2 cut(s) 1035, 1374
Bpu1102I GCTNAGC 1 cut(s) 354
BpuEI CTTGAG 2 cut(s) 394, 1538
BpuMI CCSGG 1 cut(s) 538
BsaBI GATNNNNATC 2 cut(s) 1650, 1704
BsaI GGTCTC 1 cut(s) 1531
BsaJI CCNNGG 4 cut(s) 537, 750, 775, 886
BsaXI ACNNNNNCTCC 6 cut(s) 244, 274, 518, 548, 1222, 1252
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 269, 554, 1366
Bse1I ACTGG 5 cut(s) 152, 187, 300, 648, 1534
Bse21I CCTNAGG 1 cut(s) 201
Bse8I GATNNNNATC 2 cut(s) 1650, 1704
BseDI CCNNGG 4 cut(s) 537, 750, 775, 886
BseGI GGATG 4 cut(s) 196, 955, 1408, 1593
BseJI GATNNNNATC 2 cut(s) 1650, 1704
BseLI CCNNNNNNNGG 4 cut(s) 202, 269, 554, 1366
BseMII CTCAG 7 cut(s) 45, 154, 215, 292, 345, 554, 1812
BseNI ACTGG 5 cut(s) 152, 187, 300, 648, 1534
BseSI GKGCMC 3 cut(s) 624, 1311, 1697
BseX3I CGGCCG 1 cut(s) 487
BseXI GCAGC 3 cut(s) 56, 701, 1672
BseYI CCCAGC 1 cut(s) 263
BsgI GTGCAG 1 cut(s) 1660
Bsh1285I CGRYCG 1 cut(s) 490
BshFI GGCC 4 cut(s) 489, 612, 821, 1720
BsiEI CGRYCG 1 cut(s) 490
BsiHKAI GWGCWC 3 cut(s) 624, 1311, 1697
BsiSI CCGG 1 cut(s) 537
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 4 cut(s) 202, 269, 554, 1366
BsmAI GTCTC 3 cut(s) 286, 1369, 1531
BsmFI GGGAC 1 cut(s) 265
BsnI GGCC 4 cut(s) 489, 612, 821, 1720
Bso31I GGTCTC 1 cut(s) 1531
Bsp1286I GDGCHC 5 cut(s) 624, 793, 1311, 1359, 1697
Bsp1720I GCTNAGC 1 cut(s) 354
Bsp19I CCATGG 2 cut(s) 750, 886
BspACI CCGC 5 cut(s) 490, 505, 671, 1467, 1636
BspANI GGCC 4 cut(s) 489, 612, 821, 1720
BspCNI CTCAG 7 cut(s) 44, 153, 214, 291, 346, 555, 1811
BspHI TCATGA 1 cut(s) 993
BspLI GGNNCC 3 cut(s) 26, 1133, 1356
BspMAI CTGCAG 1 cut(s) 1130
BspPI GGATC 4 cut(s) 967, 1054, 1541, 1646
BspQI GCTCTTC 1 cut(s) 1206
BspTNI GGTCTC 1 cut(s) 1531
BsrBI CCGCTC 1 cut(s) 507
BsrI ACTGG 5 cut(s) 152, 187, 300, 648, 1534
BssECI CCNNGG 4 cut(s) 537, 750, 775, 886
BssNAI GTATAC 1 cut(s) 831
BssT1I CCWWGG 3 cut(s) 750, 775, 886
Bst1107I GTATAC 1 cut(s) 831
Bst4CI ACNGT 6 cut(s) 52, 128, 979, 1313, 1420, 1743
Bst6I CTCTTC 3 cut(s) 770, 939, 1206
BstC8I GCNNGC 3 cut(s) 865, 1130, 1429
BstDEI CTNAG 9 cut(s) 31, 140, 201, 278, 354, 359, 563, 1180, 1798
BstDSI CCRYGG 2 cut(s) 750, 886
BstF5I GGATG 4 cut(s) 196, 955, 1408, 1593
BstH2I RGCGCY 1 cut(s) 302
BstHHI GCGC 2 cut(s) 221, 301
BstMAI GTCTC 3 cut(s) 286, 1369, 1531
BstMCI CGRYCG 1 cut(s) 490
BstMWI GCNNNNNNNGC 3 cut(s) 350, 1213, 1437
BstNSI RCATGY 1 cut(s) 139
BstPAI GACNNNNGTC 2 cut(s) 1035, 1374
BstSCI CCNGG 1 cut(s) 536
BstSFI CTRYAG 1 cut(s) 1126
BstSLI GKGCMC 3 cut(s) 624, 1311, 1697
BstV1I GCAGC 3 cut(s) 56, 701, 1672
BstX2I RGATCY 3 cut(s) 892, 1077, 1651
BstYI RGATCY 3 cut(s) 892, 1077, 1651
BstZ17I GTATAC 1 cut(s) 831
BstZI CGGCCG 1 cut(s) 487
Bsu36I CCTNAGG 1 cut(s) 201
BsuRI GGCC 4 cut(s) 489, 612, 821, 1720
BtgI CCRYGG 2 cut(s) 750, 886
BtsCI GGATG 4 cut(s) 196, 955, 1408, 1593
BtsIMutI CAGTG 3 cut(s) 641, 975, 1425
Cac8I GCNNGC 3 cut(s) 865, 1130, 1429
CaiI CAGNNNCTG 3 cut(s) 50, 147, 295
CciI TCATGA 1 cut(s) 993
CfoI GCGC 2 cut(s) 221, 301
Cfr13I GGNCC 2 cut(s) 25, 205
Csp6I GTAC 3 cut(s) 370, 982, 1276
CspCI CAANNNNNGTGG 2 cut(s) 1405, 1440
CviQI GTAC 3 cut(s) 370, 982, 1276
DdeI CTNAG 9 cut(s) 31, 140, 201, 278, 354, 359, 563, 1180, 1798
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
EaeI YGGCCR 3 cut(s) 487, 610, 819
EagI CGGCCG 1 cut(s) 487
Eam1104I CTCTTC 3 cut(s) 770, 939, 1206
EarI CTCTTC 3 cut(s) 770, 939, 1206
EclXI CGGCCG 1 cut(s) 487
Eco130I CCWWGG 3 cut(s) 750, 775, 886
Eco24I GRGCYC 2 cut(s) 793, 1359
Eco31I GGTCTC 1 cut(s) 1531
Eco47I GGWCC 2 cut(s) 25, 205
Eco52I CGGCCG 1 cut(s) 487
Eco81I CCTNAGG 1 cut(s) 201
EcoO109I RGGNCCY 1 cut(s) 25
EcoT14I CCWWGG 3 cut(s) 750, 775, 886
EcoT38I GRGCYC 2 cut(s) 793, 1359
ErhI CCWWGG 3 cut(s) 750, 775, 886
FalI AAGNNNNNCTT 4 cut(s) 75, 107, 1659, 1691
FaqI GGGAC 1 cut(s) 265
FauI CCCGC 1 cut(s) 1460
FblI GTMKAC 1 cut(s) 830
Fnu4HI GCNGC 5 cut(s) 45, 490, 672, 690, 1686
FokI GGATG 4 cut(s) 183, 962, 1415, 1600
FriOI GRGCYC 2 cut(s) 793, 1359
Fsp4HI GCNGC 5 cut(s) 45, 490, 672, 690, 1686
FspBI CTAG 4 cut(s) 245, 1106, 1326, 1748
GlaI GCGC 2 cut(s) 220, 300
GluI GCNGC 5 cut(s) 45, 490, 672, 690, 1686
GsaI CCCAGC 1 cut(s) 267
HaeII RGCGCY 1 cut(s) 302
HaeIII GGCC 4 cut(s) 489, 612, 821, 1720
HapII CCGG 1 cut(s) 537
HhaI GCGC 2 cut(s) 221, 301
Hin6I GCGC 2 cut(s) 219, 299
HinP1I GCGC 2 cut(s) 219, 299
HincII GTYRAC 2 cut(s) 1111, 1630
HindII GTYRAC 2 cut(s) 1111, 1630
HindIII AAGCTT 2 cut(s) 1145, 1385
HpaI GTTAAC 1 cut(s) 1111
HpaII CCGG 1 cut(s) 537
HphI GGTGA 4 cut(s) 70, 159, 296, 1758
Hpy166II GTNNAC 9 cut(s) 522, 622, 747, 831, 982, 1111, 1309, 1630, 1695
Hpy188I TCNGA 9 cut(s) 160, 409, 513, 564, 1051, 1224, 1369, 1489, 1801
Hpy8I GTNNAC 9 cut(s) 522, 622, 747, 831, 982, 1111, 1309, 1630, 1695
HpyAV CCTTC 3 cut(s) 486, 915, 1037
HpyCH4III ACNGT 6 cut(s) 52, 128, 979, 1313, 1420, 1743
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 1213, 1437
HpyF3I CTNAG 9 cut(s) 31, 140, 201, 278, 354, 359, 563, 1180, 1798
HspAI GCGC 2 cut(s) 219, 299
KspAI GTTAAC 1 cut(s) 1111
LguI GCTCTTC 1 cut(s) 1206
LmnI GCTCC 3 cut(s) 1137, 1176, 1354
Lsp1109I GCAGC 3 cut(s) 56, 701, 1672
LweI GCATC 3 cut(s) 1393, 1636, 1690
MaeI CTAG 4 cut(s) 245, 1106, 1326, 1748
MaeIII GTNAC 8 cut(s) 143, 795, 838, 933, 1313, 1411, 1525, 1764
MbiI CCGCTC 1 cut(s) 507
MboII GAAGA 6 cut(s) 16, 523, 699, 757, 956, 1223
MflI RGATCY 3 cut(s) 892, 1077, 1651
MhlI GDGCHC 5 cut(s) 624, 793, 1311, 1359, 1697
MlsI TGGCCA 2 cut(s) 612, 821
MluCI AATT 9 cut(s) 175, 222, 339, 382, 454, 926, 1026, 1225, 1671
MluNI TGGCCA 2 cut(s) 612, 821
MlyI GAGTC 4 cut(s) 222, 495, 941, 1784
MmeI TCCRAC 3 cut(s) 535, 1565, 1574
Mox20I TGGCCA 2 cut(s) 612, 821
MroXI GAANNNNTTC 2 cut(s) 421, 1671
MscI TGGCCA 2 cut(s) 612, 821
MseI TTAA 7 cut(s) 225, 461, 720, 1092, 1110, 1241, 1571
MslI CAYNNNNRTG 1 cut(s) 1200
Msp20I TGGCCA 2 cut(s) 612, 821
MspI CCGG 1 cut(s) 537
MspR9I CCNGG 1 cut(s) 538
MwoI GCNNNNNNNGC 3 cut(s) 350, 1213, 1437
NciI CCSGG 1 cut(s) 538
NcoI CCATGG 2 cut(s) 750, 886
NlaIV GGNNCC 3 cut(s) 26, 1133, 1356
NmuCI GTSAC 4 cut(s) 933, 1313, 1525, 1764
NspI RCATGY 1 cut(s) 139
PagI TCATGA 1 cut(s) 993
PciSI GCTCTTC 1 cut(s) 1206
PdmI GAANNNNTTC 2 cut(s) 421, 1671
PfeI GAWTC 7 cut(s) 155, 164, 324, 595, 683, 853, 1729
PflMI CCANNNNNTGG 1 cut(s) 554
PkrI GCNGC 5 cut(s) 46, 491, 673, 691, 1687
PleI GAGTC 4 cut(s) 221, 495, 940, 1784
PpsI GAGTC 4 cut(s) 221, 495, 940, 1784
PpuMI RGGWCCY 1 cut(s) 25
PshAI GACNNNNGTC 2 cut(s) 1035, 1374
Psp5II RGGWCCY 1 cut(s) 25
PspFI CCCAGC 1 cut(s) 263
PspN4I GGNNCC 3 cut(s) 26, 1133, 1356
PspPI GGNCC 2 cut(s) 25, 205
PspPPI RGGWCCY 1 cut(s) 25
PstI CTGCAG 1 cut(s) 1130
PstNI CAGNNNCTG 3 cut(s) 50, 147, 295
PsuI RGATCY 3 cut(s) 892, 1077, 1651
RsaI GTAC 3 cut(s) 371, 983, 1277
RsaNI GTAC 3 cut(s) 370, 982, 1276
RseI CAYNNNNRTG 1 cut(s) 1200
SapI GCTCTTC 1 cut(s) 1206
SaqAI TTAA 7 cut(s) 225, 461, 720, 1092, 1110, 1241, 1571
SatI GCNGC 5 cut(s) 45, 490, 672, 690, 1686
Sau96I GGNCC 2 cut(s) 25, 205
SchI GAGTC 4 cut(s) 222, 495, 941, 1784
ScrFI CCNGG 1 cut(s) 538
SduI GDGCHC 5 cut(s) 624, 793, 1311, 1359, 1697
SfaNI GCATC 3 cut(s) 1393, 1636, 1690
SfcI CTRYAG 1 cut(s) 1126
SinI GGWCC 2 cut(s) 25, 205
SmiMI CAYNNNNRTG 1 cut(s) 1200
SmlI CTYRAG 2 cut(s) 373, 1517
SmoI CTYRAG 2 cut(s) 373, 1517
Sse9I AATT 9 cut(s) 175, 222, 339, 382, 454, 926, 1026, 1225, 1671
SsiI CCGC 5 cut(s) 490, 505, 671, 1467, 1636
SspMI CTAG 4 cut(s) 245, 1106, 1326, 1748
StyD4I CCNGG 1 cut(s) 536
StyI CCWWGG 3 cut(s) 750, 775, 886
TaaI ACNGT 6 cut(s) 52, 128, 979, 1313, 1420, 1743
TaqI TCGA 3 cut(s) 634, 803, 1062
TasI AATT 9 cut(s) 175, 222, 339, 382, 454, 926, 1026, 1225, 1671
TatI WGTACW 1 cut(s) 369
TauI GCSGC 2 cut(s) 492, 674
TfiI GAWTC 7 cut(s) 155, 164, 324, 595, 683, 853, 1729
Tru1I TTAA 7 cut(s) 225, 461, 720, 1092, 1110, 1241, 1571
Tru9I TTAA 7 cut(s) 225, 461, 720, 1092, 1110, 1241, 1571
TscAI CASTG 3 cut(s) 648, 982, 1425
TseFI GTSAC 4 cut(s) 933, 1313, 1525, 1764
TseI GCWGC 3 cut(s) 44, 689, 1685
Tsp45I GTSAC 4 cut(s) 933, 1313, 1525, 1764
TspGWI ACGGA 2 cut(s) 336, 1226
TspRI CASTG 3 cut(s) 648, 982, 1425
Van91I CCANNNNNTGG 1 cut(s) 554
VneI GTGCAC 3 cut(s) 620, 1307, 1693
VpaK11BI GGWCC 2 cut(s) 25, 205
XceI RCATGY 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 830
XmnI GAANNNNTTC 2 cut(s) 421, 1671
XspI CTAG 4 cut(s) 245, 1106, 1326, 1748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.