pycom14g16110

LRR receptor-like serine threonine-protein kinase FEI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009488
Physical Location & Seq
Forward (+)
18512964 .. 18514391
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g16110.2

Sequence Viewer

Length: 873 bp
ATGAAGAGGATGTTGTCAGGATCGGGAGGATTTGGCACTGTATACAGAATGGTTATGAATGATTGTGGTACTTTTGCTGTTAAAAGAATTGATAGAAGTCGGGAAGGATGTGATCAAGTTTTTGAGAGGGAATTAGAGATCTTGGGTAGCATTAAGCACATAAATCTAGTTAACCTGCGAGGCTACTGCAGGCTCCCTGTTTCAAAGCTTCTCATATATGATTATCTAGCTATGGGCAGTTTAGATGATTTCTTGCATGAGCATTGGGTGGAAGAGCGGCCTTTGAACTGGAATGCTCGTTTGAAAATTGCCCTGGGTTCTGCTCGAGGCATAGCATACCTGCACACTGACTGCTCTCCAAAGATAGTACACCGTGACATAAAATCGAGCAATATTCTTCTAAATGAAAACTTGGATCCCCATGTCTCTGACTTTGGCCTTGCAAAGCTTTTGGTGGATGAGGATGCCCATATTACGACAGTGGTGGCTGGCACCTTTGGCTATCTGGCTCCAGAGTATCTGCAAAGTGGGAGAGCCACTCAGAAGACAGATGTATATAGCTTTGGAGTTCTATTGCTAGAGCTTGTGACCGGAAAAAGACCTACTGATCCTACGTTTGTAAAACGAGGCTTAAATGTTGTTGGTTGGATGAACACCCAATTGAGAGAAAACAGGTTGCATGAAGTAGTAGACAAAAGGTGCAAAGATGCAGATGCTGAAACTGTGGAAGCAATTCTTGATATCGCGGCAAGGTGTACCGATGCAAACCCTGATGACCGGCCATCAATGAACCAGGTATTGCAGTTGCTAGAGCCAGAGGTCATGTCACCGTGCCCGAGCGATTTCTACGAGTCTCATTCAGATCATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

291

Amino Acids

32.73

Weight (kDa)

5.7

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 183, 348
AccB1I GGYRCC 1 cut(s) 491
AccBSI CCGCTC 1 cut(s) 277
AccI GTMKAC 2 cut(s) 42, 690
AccII CGCG 1 cut(s) 746
AciI CCGC 2 cut(s) 277, 746
AclWI GGATC 4 cut(s) 28, 410, 423, 602
AcoI YGGCCR 1 cut(s) 779
AfaI GTAC 3 cut(s) 70, 369, 757
AgsI TTSAA 3 cut(s) 204, 286, 304
AjnI CCWGG 2 cut(s) 312, 792
AluBI AGCT 5 cut(s) 208, 230, 448, 561, 583
AluI AGCT 5 cut(s) 208, 230, 448, 561, 583
Alw26I GTCTC 2 cut(s) 430, 858
AlwI GGATC 4 cut(s) 28, 410, 423, 602
AlwNI CAGNNNCTG 1 cut(s) 716
Ama87I CYCGRG 2 cut(s) 324, 835
AoxI GGCC 3 cut(s) 278, 436, 779
Asp700I GAANNNNTTC 1 cut(s) 732
AsuHPI GGTGA 1 cut(s) 819
AvaI CYCGRG 2 cut(s) 324, 835
BaeGI GKGCMC 1 cut(s) 836
BamHI GGATCC 1 cut(s) 415
BanI GGYRCC 1 cut(s) 491
BbsI GAAGAC 1 cut(s) 551
BccI CCATC 1 cut(s) 790
BcgI CGANNNNNNTGC 2 cut(s) 168, 202
BciT130I CCWGG 2 cut(s) 314, 794
BclI TGATCA 1 cut(s) 112
BcoDI GTCTC 2 cut(s) 430, 858
BfaI CTAG 4 cut(s) 167, 227, 578, 809
BfmI CTRYAG 1 cut(s) 187
BfuAI ACCTGC 2 cut(s) 183, 348
BglII AGATCT 1 cut(s) 138
BisI GCNGC 2 cut(s) 278, 747
BlsI GCNGC 2 cut(s) 279, 748
Bme1390I CCNGG 2 cut(s) 314, 794
BmeT110I CYCGRG 2 cut(s) 324, 835
BmiI GGNNCC 4 cut(s) 194, 417, 493, 510
BmrFI CCNGG 2 cut(s) 314, 794
BmsI GCATC 4 cut(s) 454, 697, 703, 751
BpiI GAAGAC 1 cut(s) 551
BplI GAGNNNNNCTC 2 cut(s) 523, 555
BpmI CTGGAG 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 312, 313
BsaWI WCCGGW 1 cut(s) 590
Bse118I RCCGGY 1 cut(s) 777
Bse1I ACTGG 1 cut(s) 293
BseBI CCWGG 2 cut(s) 314, 794
BseDI CCNNGG 2 cut(s) 312, 313
BseGI GGATG 5 cut(s) 15, 113, 463, 469, 654
BseMII CTCAG 1 cut(s) 554
BseNI ACTGG 1 cut(s) 293
BseSI GKGCMC 1 cut(s) 836
BsgI GTGCAG 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 746
BshFI GGCC 3 cut(s) 280, 438, 781
BshNI GGYRCC 1 cut(s) 491
BsiHKCI CYCGRG 2 cut(s) 324, 835
BsiSI CCGG 2 cut(s) 591, 778
BsmAI GTCTC 2 cut(s) 430, 858
BsmI GAATGC 1 cut(s) 298
BsnI GGCC 3 cut(s) 280, 438, 781
BsoBI CYCGRG 2 cut(s) 324, 835
Bsp1286I GDGCHC 1 cut(s) 836
Bsp143I GATC 6 cut(s) 20, 112, 138, 415, 607, 862
BspACI CCGC 2 cut(s) 277, 746
BspANI GGCC 3 cut(s) 280, 438, 781
BspCNI CTCAG 1 cut(s) 553
BspFNI CGCG 1 cut(s) 746
BspLI GGNNCC 4 cut(s) 194, 417, 493, 510
BspMAI CTGCAG 1 cut(s) 191
BspMI ACCTGC 2 cut(s) 183, 348
BspPI GGATC 4 cut(s) 28, 410, 423, 602
BspQI GCTCTTC 1 cut(s) 267
BspT107I GGYRCC 1 cut(s) 491
BsrBI CCGCTC 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 777
BsrI ACTGG 1 cut(s) 293
BssAI RCCGGY 1 cut(s) 777
BssECI CCNNGG 2 cut(s) 312, 313
BssMI GATC 6 cut(s) 20, 112, 138, 415, 607, 862
BssNAI GTATAC 1 cut(s) 43
Bst1107I GTATAC 1 cut(s) 43
Bst2UI CCWGG 2 cut(s) 314, 794
Bst4CI ACNGT 5 cut(s) 40, 374, 481, 724, 831
Bst6I CTCTTC 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 191, 490
BstDEI CTNAG 1 cut(s) 540
BstF5I GGATG 5 cut(s) 15, 113, 463, 469, 654
BstFNI CGCG 1 cut(s) 746
BstKTI GATC 6 cut(s) 23, 115, 141, 418, 610, 865
BstMAI GTCTC 2 cut(s) 430, 858
BstMBI GATC 6 cut(s) 20, 112, 138, 415, 607, 862
BstMWI GCNNNNNNNGC 1 cut(s) 498
BstNI CCWGG 2 cut(s) 314, 794
BstSCI CCNGG 2 cut(s) 312, 792
BstSFI CTRYAG 1 cut(s) 187
BstSLI GKGCMC 1 cut(s) 836
BstUI CGCG 1 cut(s) 746
BstV2I GAAGAC 1 cut(s) 551
BstX2I RGATCY 2 cut(s) 138, 415
BstYI RGATCY 2 cut(s) 138, 415
BstZ17I GTATAC 1 cut(s) 43
BsuRI GGCC 3 cut(s) 280, 438, 781
BtsCI GGATG 5 cut(s) 15, 113, 463, 469, 654
BtsIMutI CAGTG 3 cut(s) 36, 345, 486
BveI ACCTGC 2 cut(s) 183, 348
Cac8I GCNNGC 2 cut(s) 191, 490
CaiI CAGNNNCTG 1 cut(s) 716
Cfr10I RCCGGY 1 cut(s) 777
CsiI ACCWGGT 1 cut(s) 792
Csp6I GTAC 3 cut(s) 69, 368, 756
CviAII CATG 4 cut(s) 257, 422, 680, 823
CviQI GTAC 3 cut(s) 69, 368, 756
DdeI CTNAG 1 cut(s) 540
DpnI GATC 6 cut(s) 22, 114, 140, 417, 609, 864
DpnII GATC 6 cut(s) 20, 112, 138, 415, 607, 862
EaeI YGGCCR 1 cut(s) 779
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Eco32I GATATC 1 cut(s) 742
Eco88I CYCGRG 2 cut(s) 324, 835
EcoRII CCWGG 2 cut(s) 312, 792
EcoRV GATATC 1 cut(s) 742
FaeI CATG 4 cut(s) 260, 425, 683, 826
FalI AAGNNNNNCTT 2 cut(s) 720, 752
FatI CATG 4 cut(s) 256, 421, 679, 822
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 2 cut(s) 42, 690
Fnu4HI GCNGC 2 cut(s) 278, 747
FokI GGATG 5 cut(s) 22, 120, 470, 476, 661
Fsp4HI GCNGC 2 cut(s) 278, 747
FspBI CTAG 4 cut(s) 167, 227, 578, 809
GluI GCNGC 2 cut(s) 278, 747
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 3 cut(s) 280, 438, 781
HapII CCGG 2 cut(s) 591, 778
Hin1II CATG 4 cut(s) 260, 425, 683, 826
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 2 cut(s) 206, 446
HinfI GANTC 1 cut(s) 851
HpaI GTTAAC 1 cut(s) 172
HpaII CCGG 2 cut(s) 591, 778
HphI GGTGA 1 cut(s) 819
Hpy166II GTNNAC 5 cut(s) 43, 172, 370, 691, 756
Hpy188I TCNGA 3 cut(s) 430, 543, 862
Hpy188III TCNNGA 5 cut(s) 18, 24, 101, 512, 737
Hpy8I GTNNAC 5 cut(s) 43, 172, 370, 691, 756
HpyAV CCTTC 1 cut(s) 98
HpyCH4III ACNGT 5 cut(s) 40, 374, 481, 724, 831
HpyCH4IV ACGT 1 cut(s) 614
HpyF10VI GCNNNNNNNGC 1 cut(s) 498
HpyF3I CTNAG 1 cut(s) 540
HpySE526I ACGT 1 cut(s) 614
Hsp92II CATG 4 cut(s) 260, 425, 683, 826
Ksp22I TGATCA 1 cut(s) 112
KspAI GTTAAC 1 cut(s) 172
Kzo9I GATC 6 cut(s) 20, 112, 138, 415, 607, 862
LguI GCTCTTC 1 cut(s) 267
LmnI GCTCC 2 cut(s) 198, 514
LweI GCATC 4 cut(s) 454, 697, 703, 751
MabI ACCWGGT 1 cut(s) 792
MaeI CTAG 4 cut(s) 167, 227, 578, 809
MaeII ACGT 1 cut(s) 614
MaeIII GTNAC 3 cut(s) 374, 586, 825
MalI GATC 6 cut(s) 22, 114, 140, 417, 609, 864
MbiI CCGCTC 1 cut(s) 277
MboI GATC 6 cut(s) 20, 112, 138, 415, 607, 862
MboII GAAGA 4 cut(s) 16, 284, 389, 556
MfeI CAATTG 1 cut(s) 659
MflI RGATCY 2 cut(s) 138, 415
MhlI GDGCHC 1 cut(s) 836
MluCI AATT 5 cut(s) 87, 131, 306, 659, 732
MlyI GAGTC 1 cut(s) 860
MmeI TCCRAC 1 cut(s) 626
MnlI CCTC 7 cut(s) 20, 120, 173, 320, 454, 620, 811
MroXI GAANNNNTTC 1 cut(s) 732
MseI TTAA 4 cut(s) 81, 153, 171, 632
MspI CCGG 2 cut(s) 591, 778
MspR9I CCNGG 2 cut(s) 314, 794
MunI CAATTG 1 cut(s) 659
Mva1269I GAATGC 1 cut(s) 298
MvaI CCWGG 2 cut(s) 314, 794
MvnI CGCG 1 cut(s) 746
MwoI GCNNNNNNNGC 1 cut(s) 498
NdeII GATC 6 cut(s) 20, 112, 138, 415, 607, 862
NlaIII CATG 4 cut(s) 260, 425, 683, 826
NlaIV GGNNCC 4 cut(s) 194, 417, 493, 510
NmuCI GTSAC 3 cut(s) 374, 586, 825
PaeR7I CTCGAG 1 cut(s) 324
PasI CCCWGGG 1 cut(s) 313
PciSI GCTCTTC 1 cut(s) 267
PctI GAATGC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 732
PkrI GCNGC 2 cut(s) 279, 748
PleI GAGTC 1 cut(s) 859
PpsI GAGTC 1 cut(s) 859
Psp6I CCWGG 2 cut(s) 312, 792
PspGI CCWGG 2 cut(s) 312, 792
PspN4I GGNNCC 4 cut(s) 194, 417, 493, 510
PspXI VCTCGAGB 1 cut(s) 324
PstI CTGCAG 1 cut(s) 191
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 2 cut(s) 138, 415
RsaI GTAC 3 cut(s) 70, 369, 757
RsaNI GTAC 3 cut(s) 69, 368, 756
SapI GCTCTTC 1 cut(s) 267
SaqAI TTAA 4 cut(s) 81, 153, 171, 632
SatI GCNGC 2 cut(s) 278, 747
Sau3AI GATC 6 cut(s) 20, 112, 138, 415, 607, 862
SchI GAGTC 1 cut(s) 860
ScrFI CCNGG 2 cut(s) 314, 794
SduI GDGCHC 1 cut(s) 836
SexAI ACCWGGT 1 cut(s) 792
SfaNI GCATC 4 cut(s) 454, 697, 703, 751
SfcI CTRYAG 1 cut(s) 187
Sfr274I CTCGAG 1 cut(s) 324
SlaI CTCGAG 1 cut(s) 324
SmlI CTYRAG 1 cut(s) 324
SmoI CTYRAG 1 cut(s) 324
Sse9I AATT 5 cut(s) 87, 131, 306, 659, 732
SsiI CCGC 2 cut(s) 277, 746
SspI AATATT 1 cut(s) 394
SspMI CTAG 4 cut(s) 167, 227, 578, 809
StyD4I CCNGG 2 cut(s) 312, 792
TaaI ACNGT 5 cut(s) 40, 374, 481, 724, 831
TaiI ACGT 1 cut(s) 617
TaqI TCGA 2 cut(s) 325, 386
TasI AATT 5 cut(s) 87, 131, 306, 659, 732
TatI WGTACW 1 cut(s) 367
TauI GCSGC 2 cut(s) 280, 749
Tru1I TTAA 4 cut(s) 81, 153, 171, 632
Tru9I TTAA 4 cut(s) 81, 153, 171, 632
TscAI CASTG 3 cut(s) 43, 352, 486
TseFI GTSAC 3 cut(s) 374, 586, 825
Tsp45I GTSAC 3 cut(s) 374, 586, 825
TspDTI ATGAA 6 cut(s) 17, 71, 420, 665, 696, 803
TspRI CASTG 3 cut(s) 43, 352, 486
XhoI CTCGAG 1 cut(s) 324
XmiI GTMKAC 2 cut(s) 42, 690
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 4 cut(s) 167, 227, 578, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.