AT5G63390

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012228
Physical Location & Seq
Reverse (-)
25390249 .. 25392684
2436 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63390.1

Sequence Viewer

Length: 1680 bp
ATGGCGAAATCGATGAGAACTATCAAAAATCCATTCTTTACTTCGCATCCGCCATCTCCATTCTTCCATCTCTCTCTCCTCTTCTTCACTCCCCTGAAGAAATCAAGTCCCCGATACCTAAACCACCATCACCAAACTCGATGCCCACCTCCACAGTTTTTCCTTCTTCTCATCTCTCTTTCTCTCGTTTTCTCGGGAATTTCATTTCTCACATTTTCTTTAGACACTGGATCGTCAACTTGCGTCTCCACATCTTCCGCTTCCTCTCTCAAATTCTTCATCTCCGATGTCGATCACCGTCAGATCTTAACCTCCCTTGCTGTATCCGGAGCTTCCACGTTGTTTCCGTTACCGGCGAGAGGAGGACATTCCGGGAATATGACGGAGGAAGAGAAAGAGTTTTGGAAACAACCCAATGGTGAAGGATACAAGCCTTGTTTGGATTTTAGTTTGGAGTACAAGAAGAAATCAGCTAGTGTTTCTAAAGAGAAGAAACGATTCCTTGTTGTTGTTGTCTCTGGTGGACTTAATCAACAACGGAATCAAATCGTTGATGCTGTTGTAATCGCTATGATTCTTGAAGCTGCTCTTGTTGTTCCTGTTTTGCAAGTGAATCGAGTCTGGGGAGACGAAAGTGAATTCTCAGATCTTTTCGATGTAGAGCATTTCAAGAAAACGCTGAGATCTGATGTTCGTATCGTTTCTTCTCTACCGTCAACGCATCTTATGTCTAGACAAACAATTGAGAATCAGATTCCTTGGGATGTTTCTCCGGTTTGGATCCGTGCTAAATACTTCAAGCAGTTGAATGAGGAAGGACTTTTGGTGTTGAAAGGATTGGACTCAAAGTTAGCCAAGAATCTACCGCCGGATCTGCAGAAACTTCGATGCAAGGTTGCTTTCCATGCACTGAGATTCGCGGCACCAATTGAGAATCTCGGGAACAAACTCACGAGAAGAATGTGGATAGAAGGTCCATATATTGCTCTCCATTTAAGGCTAGAGAAAGATGTTTGGGTAAGAACCGGTTGTCTCACTGGATTAGGCTCAGAATTCGATCGGATCATAGCAGAAACCAGAACGTCTCAGCCTCGATACCTCACTGGTAGACTAAACTTGACCTATACAGAGCGTAGACTTGCTGGTTTTTGTCCTCTCAATGTATATGAGATTGCAAGGTTGTTGAAGGCTTTGGGAGCACCTAGTAATGCGTCGATATATATTGCAGGAGGTGAACCATTTGGTGGCTCACGAGCATTAGAGCCTCTCGCTAAAGAGTTCTCTAATTTGGTGACAAAGGAGACATTGGCTCATAAAGGCGAGCTCTTGCCTTACACCAATAGATCTTCAGCTTTAGCAGCTATTGACTACATTGTATCACTTAGCAGCGATGTTTTTATTCCTTCCCATGGTGGAAACATGGCAAAAGCTATGCAGGGAAATAGAGCTTATGTAGGACACAGGAAGTTTATAATGCCTAATAAGAGAGCGATGCTTCCTTTAATGGAGAATTCTTCTGTTTCAGATGCCGAGCTCAGTTTTCTAACTCGGAAGCTTCACCGTAAGTCCCAAGGCTATCCTGAGTCACGGAGAGGGCGGAGAGATCGTGACGTGATAGCTTATCCTGTCCCGGAGTGTATGTGTCGACATAGAAAACACAGGTCTGTAGGTTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

559

Amino Acids

62.89

Weight (kDa)

9.76

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 167 - 484 4.5e-87 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63390
fragaria_vesca FvH4_5g07850
malus_domestica MD06G1145500.v1.1 MD14G1160200.v1.1
prunus_persica Prupe.5G153800_v2.0.a1
pyrus_communis pycom06g13480
rosa_chinensis RchiOBHm_Chr7g0190611
rosa_laevigata RLG00000004510
rosa_multiflora Rmu_sc0006709.1_g000010 Rmu_sc0023847.1_g000002 Rmu_sc0026450.1_g000001
rosa_roxburghii Rroxscaffold_3G00264460
rosa_rugosa Rorug06G0505600 Rorug06G0505700
rosa_samantha Rh7AG112300 Rh7BG115100 Rh7CG117200 Rh7DG116300
rosa_wichuraiana Rw7G009800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1472
AccB1I GGYRCC 1 cut(s) 922
AccB7I CCANNNNNTGG 1 cut(s) 1244
AccI GTMKAC 3 cut(s) 1108, 1135, 1645
AccII CGCG 1 cut(s) 920
AccIII TCCGGA 1 cut(s) 326
AciI CCGC 5 cut(s) 50, 258, 866, 920, 1597
AclWI GGATC 5 cut(s) 238, 775, 788, 879, 1070
AcsI RAATTY 5 cut(s) 198, 272, 638, 1052, 1510
AcuI CTGAAG 2 cut(s) 116, 1332
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 3 cut(s) 359, 1244, 1409
AgeI ACCGGT 1 cut(s) 1025
AgsI TTSAA 6 cut(s) 581, 670, 799, 808, 832, 1186
AjiI CACGTC 1 cut(s) 1612
Alw21I GWGCWC 3 cut(s) 1201, 1326, 1536
Alw26I GTCTC 6 cut(s) 250, 520, 621, 1037, 1089, 1295
AlwI GGATC 5 cut(s) 238, 775, 788, 879, 1070
Ama87I CYCGRG 2 cut(s) 193, 938
Aor13HI TCCGGA 1 cut(s) 326
ApeKI GCWGC 3 cut(s) 584, 1358, 1386
ApoI RAATTY 5 cut(s) 198, 272, 638, 1052, 1510
AsiGI ACCGGT 1 cut(s) 1025
Asp700I GAANNNNTTC 1 cut(s) 497
AspS9I GGNCC 1 cut(s) 974
AsuC2I CCSGG 2 cut(s) 373, 1631
AsuHPI GGTGA 6 cut(s) 122, 287, 431, 1244, 1303, 1550
AvaI CYCGRG 2 cut(s) 193, 938
AvaII GGWCC 1 cut(s) 974
BamHI GGATCC 1 cut(s) 780
BanI GGYRCC 1 cut(s) 922
BanII GRGCYC 2 cut(s) 1326, 1536
BarI GAAGNNNNNNTAC 2 cut(s) 688, 720
BauI CACGAG 2 cut(s) 952, 1251
Bbv12I GWGCWC 3 cut(s) 1201, 1326, 1536
BbvI GCAGC 3 cut(s) 571, 1370, 1398
BccI CCATC 3 cut(s) 61, 75, 135
BcgI CGANNNNNNTGC 4 cut(s) 596, 630, 866, 900
BciVI GTATCC 2 cut(s) 334, 419
BcnI CCSGG 2 cut(s) 373, 1631
BcoDI GTCTC 6 cut(s) 250, 520, 621, 1037, 1089, 1295
BfaI CTAG 5 cut(s) 474, 732, 1001, 1203, 1678
BfmI CTRYAG 2 cut(s) 875, 1665
BfuI GTATCC 2 cut(s) 334, 419
BglII AGATCT 4 cut(s) 303, 646, 683, 1343
BisI GCNGC 4 cut(s) 585, 921, 1359, 1387
BlsI GCNGC 4 cut(s) 586, 922, 1360, 1388
Bme1390I CCNGG 2 cut(s) 373, 1631
Bme18I GGWCC 1 cut(s) 974
BmeT110I CYCGRG 2 cut(s) 193, 938
BmgBI CACGTC 1 cut(s) 1612
BmgT120I GGNCC 1 cut(s) 974
BmiI GGNNCC 2 cut(s) 782, 924
BmrFI CCNGG 2 cut(s) 373, 1631
BmsI GCATC 7 cut(s) 55, 131, 544, 730, 878, 1482, 1516
BpuMI CCSGG 2 cut(s) 373, 1631
Bsa29I ATCGAT 1 cut(s) 11
BsaBI GATNNNNATC 1 cut(s) 291
BsaJI CCNNGG 3 cut(s) 758, 1408, 1570
BsaWI WCCGGW 3 cut(s) 326, 772, 1025
BsaXI ACNNNNNCTCC 4 cut(s) 1591, 1621, 1625, 1655
Bsc4I CCNNNNNNNGG 3 cut(s) 359, 1244, 1409
Bse118I RCCGGY 2 cut(s) 352, 1025
Bse1I ACTGG 3 cut(s) 232, 1042, 1108
Bse8I GATNNNNATC 1 cut(s) 291
BseAI TCCGGA 1 cut(s) 326
BseCI ATCGAT 1 cut(s) 11
BseDI CCNNGG 3 cut(s) 758, 1408, 1570
BseGI GGATG 2 cut(s) 46, 769
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 3 cut(s) 359, 1244, 1409
BseMII CTCAG 7 cut(s) 657, 671, 902, 1062, 1100, 1549, 1572
BseNI ACTGG 3 cut(s) 232, 1042, 1108
BseRI GAGGAG 2 cut(s) 68, 375
BseXI GCAGC 3 cut(s) 571, 1370, 1398
Bsh1236I CGCG 1 cut(s) 920
Bsh1285I CGRYCG 1 cut(s) 1060
BshNI GGYRCC 1 cut(s) 922
BshTI ACCGGT 1 cut(s) 1025
BshVI ATCGAT 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 1060
BsiHKAI GWGCWC 3 cut(s) 1201, 1326, 1536
BsiHKCI CYCGRG 2 cut(s) 193, 938
BsiSI CCGG 7 cut(s) 327, 353, 372, 773, 869, 1026, 1631
BslFI GGGAC 3 cut(s) 93, 1552, 1613
BslI CCNNNNNNNGG 3 cut(s) 359, 1244, 1409
BsmAI GTCTC 6 cut(s) 250, 520, 621, 1037, 1089, 1295
BsmBI CGTCTC 3 cut(s) 250, 621, 1089
BsmFI GGGAC 3 cut(s) 93, 1552, 1613
BsoBI CYCGRG 2 cut(s) 193, 938
Bsp1286I GDGCHC 3 cut(s) 1201, 1326, 1536
Bsp13I TCCGGA 1 cut(s) 326
Bsp19I CCATGG 1 cut(s) 1408
BspACI CCGC 5 cut(s) 50, 258, 866, 920, 1597
BspCNI CTCAG 7 cut(s) 656, 672, 903, 1061, 1099, 1548, 1573
BspDI ATCGAT 1 cut(s) 11
BspEI TCCGGA 1 cut(s) 326
BspFNI CGCG 1 cut(s) 920
BspLI GGNNCC 2 cut(s) 782, 924
BspMAI CTGCAG 1 cut(s) 879
BspPI GGATC 5 cut(s) 238, 775, 788, 879, 1070
BspT107I GGYRCC 1 cut(s) 922
BsrFI RCCGGY 2 cut(s) 352, 1025
BsrI ACTGG 3 cut(s) 232, 1042, 1108
BssAI RCCGGY 2 cut(s) 352, 1025
BssECI CCNNGG 3 cut(s) 758, 1408, 1570
BssSI CACGAG 2 cut(s) 952, 1251
BssT1I CCWWGG 3 cut(s) 758, 1408, 1570
Bst2BI CACGAG 2 cut(s) 952, 1251
Bst4CI ACNGT 4 cut(s) 156, 299, 714, 1562
Bst6I CTCTTC 2 cut(s) 86, 384
BstC8I GCNNGC 1 cut(s) 1322
BstDEI CTNAG 8 cut(s) 643, 680, 911, 1048, 1086, 1382, 1535, 1581
BstDSI CCRYGG 1 cut(s) 1408
BstF5I GGATG 2 cut(s) 46, 769
BstFNI CGCG 1 cut(s) 920
BstMAI GTCTC 6 cut(s) 250, 520, 621, 1037, 1089, 1295
BstMCI CGRYCG 1 cut(s) 1060
BstMWI GCNNNNNNNGC 4 cut(s) 874, 905, 1196, 1358
BstSCI CCNGG 2 cut(s) 371, 1629
BstSFI CTRYAG 2 cut(s) 875, 1665
BstUI CGCG 1 cut(s) 920
BstV1I GCAGC 3 cut(s) 571, 1370, 1398
BstX2I RGATCY 6 cut(s) 303, 646, 683, 780, 871, 1343
BstYI RGATCY 6 cut(s) 303, 646, 683, 780, 871, 1343
Bsu15I ATCGAT 1 cut(s) 11
BsuI GTATCC 2 cut(s) 334, 419
BsuTUI ATCGAT 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 1408
BtgZI GCGATG 2 cut(s) 1404, 1505
BtrI CACGTC 1 cut(s) 1612
BtsCI GGATG 2 cut(s) 46, 769
BtsIMutI CAGTG 4 cut(s) 225, 908, 1035, 1101
Cac8I GCNNGC 1 cut(s) 1322
Cfr10I RCCGGY 2 cut(s) 352, 1025
Cfr13I GGNCC 1 cut(s) 974
ClaI ATCGAT 1 cut(s) 11
CseI GACGC 2 cut(s) 232, 1200
Csp6I GTAC 1 cut(s) 457
CspAI ACCGGT 1 cut(s) 1025
CviAII CATG 3 cut(s) 905, 1409, 1420
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 8 cut(s) 643, 680, 911, 1048, 1086, 1382, 1535, 1581
Eam1104I CTCTTC 2 cut(s) 86, 384
EarI CTCTTC 2 cut(s) 86, 384
EciI GGCGGA 2 cut(s) 39, 1612
Ecl136II GAGCTC 2 cut(s) 1324, 1534
Eco130I CCWWGG 3 cut(s) 758, 1408, 1570
Eco24I GRGCYC 2 cut(s) 1326, 1536
Eco47I GGWCC 1 cut(s) 974
Eco53kI GAGCTC 2 cut(s) 1324, 1534
Eco57I CTGAAG 2 cut(s) 116, 1332
Eco88I CYCGRG 2 cut(s) 193, 938
EcoICRI GAGCTC 2 cut(s) 1324, 1534
EcoRI GAATTC 3 cut(s) 638, 1052, 1510
EcoT14I CCWWGG 3 cut(s) 758, 1408, 1570
EcoT38I GRGCYC 2 cut(s) 1326, 1536
ErhI CCWWGG 3 cut(s) 758, 1408, 1570
Esp3I CGTCTC 3 cut(s) 250, 621, 1089
FaeI CATG 3 cut(s) 908, 1412, 1423
FalI AAGNNNNNCTT 2 cut(s) 573, 605
FaqI GGGAC 3 cut(s) 93, 1552, 1613
FatI CATG 3 cut(s) 904, 1408, 1419
FblI GTMKAC 3 cut(s) 1108, 1135, 1645
Fnu4HI GCNGC 4 cut(s) 585, 921, 1359, 1387
FokI GGATG 2 cut(s) 33, 776
FriOI GRGCYC 2 cut(s) 1326, 1536
Fsp4HI GCNGC 4 cut(s) 585, 921, 1359, 1387
FspBI CTAG 5 cut(s) 474, 732, 1001, 1203, 1678
GluI GCNGC 4 cut(s) 585, 921, 1359, 1387
HapII CCGG 7 cut(s) 327, 353, 372, 773, 869, 1026, 1631
HgaI GACGC 2 cut(s) 232, 1200
Hin1II CATG 3 cut(s) 908, 1412, 1423
HincII GTYRAC 3 cut(s) 237, 717, 1646
HindII GTYRAC 3 cut(s) 237, 717, 1646
HindIII AAGCTT 1 cut(s) 1553
HpaII CCGG 7 cut(s) 327, 353, 372, 773, 869, 1026, 1631
HphI GGTGA 6 cut(s) 122, 287, 431, 1244, 1303, 1550
Hpy166II GTNNAC 7 cut(s) 237, 524, 717, 1109, 1136, 1235, 1646
Hpy188I TCNGA 9 cut(s) 286, 303, 646, 688, 753, 1051, 1062, 1525, 1551
Hpy8I GTNNAC 7 cut(s) 237, 524, 717, 1109, 1136, 1235, 1646
Hpy99I CGWCG 1 cut(s) 1216
HpyAV CCTTC 6 cut(s) 173, 416, 809, 965, 1180, 1413
HpyCH4III ACNGT 4 cut(s) 156, 299, 714, 1562
HpyCH4IV ACGT 3 cut(s) 338, 1082, 1611
HpyCH4V TGCA 7 cut(s) 607, 877, 891, 908, 1175, 1226, 1435
HpyF10VI GCNNNNNNNGC 4 cut(s) 874, 905, 1196, 1358
HpyF3I CTNAG 8 cut(s) 643, 680, 911, 1048, 1086, 1382, 1535, 1581
HpySE526I ACGT 3 cut(s) 338, 1082, 1611
Hsp92II CATG 3 cut(s) 908, 1412, 1423
Kpn2I TCCGGA 1 cut(s) 326
LmnI GCTCC 2 cut(s) 329, 1196
Lsp1109I GCAGC 3 cut(s) 571, 1370, 1398
LweI GCATC 7 cut(s) 55, 131, 544, 730, 878, 1482, 1516
MaeI CTAG 5 cut(s) 474, 732, 1001, 1203, 1678
MaeII ACGT 3 cut(s) 338, 1082, 1611
MaeIII GTNAC 4 cut(s) 348, 1291, 1584, 1607
MfeI CAATTG 2 cut(s) 741, 927
MflI RGATCY 6 cut(s) 303, 646, 683, 780, 871, 1343
MhlI GDGCHC 3 cut(s) 1201, 1326, 1536
MluCI AATT 8 cut(s) 198, 272, 638, 741, 927, 1052, 1285, 1510
MlyI GAGTC 3 cut(s) 627, 836, 1592
MroI TCCGGA 1 cut(s) 326
MroXI GAANNNNTTC 1 cut(s) 497
MseI TTAA 4 cut(s) 308, 528, 995, 1502
MspI CCGG 7 cut(s) 327, 353, 372, 773, 869, 1026, 1631
MspR9I CCNGG 2 cut(s) 373, 1631
MunI CAATTG 2 cut(s) 741, 927
MvnI CGCG 1 cut(s) 920
MwoI GCNNNNNNNGC 4 cut(s) 874, 905, 1196, 1358
NciI CCSGG 2 cut(s) 373, 1631
NcoI CCATGG 1 cut(s) 1408
NlaIII CATG 3 cut(s) 908, 1412, 1423
NlaIV GGNNCC 2 cut(s) 782, 924
NmeAIII GCCGAG 1 cut(s) 1555
NmuCI GTSAC 3 cut(s) 1291, 1584, 1607
PcsI WCGNNNNNNNCGW 1 cut(s) 344
PdmI GAANNNNTTC 1 cut(s) 497
PfeI GAWTC 9 cut(s) 498, 541, 574, 613, 748, 754, 859, 915, 934
PflMI CCANNNNNTGG 1 cut(s) 1244
PfoI TCCNGGA 2 cut(s) 371, 1629
PinAI ACCGGT 1 cut(s) 1025
PkrI GCNGC 4 cut(s) 586, 922, 1360, 1388
Ple19I CGATCG 1 cut(s) 1060
PleI GAGTC 3 cut(s) 626, 836, 1591
PpsI GAGTC 3 cut(s) 626, 836, 1591
PsiI TTATAA 1 cut(s) 1472
Psp124BI GAGCTC 2 cut(s) 1326, 1536
PspN4I GGNNCC 2 cut(s) 782, 924
PspPI GGNCC 1 cut(s) 974
PstI CTGCAG 1 cut(s) 879
PsuI RGATCY 6 cut(s) 303, 646, 683, 780, 871, 1343
PvuI CGATCG 1 cut(s) 1060
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
SacI GAGCTC 2 cut(s) 1326, 1536
SalI GTCGAC 1 cut(s) 1644
SaqAI TTAA 4 cut(s) 308, 528, 995, 1502
SatI GCNGC 4 cut(s) 585, 921, 1359, 1387
Sau96I GGNCC 1 cut(s) 974
SchI GAGTC 3 cut(s) 627, 836, 1592
ScrFI CCNGG 2 cut(s) 373, 1631
SduI GDGCHC 3 cut(s) 1201, 1326, 1536
SfaNI GCATC 7 cut(s) 55, 131, 544, 730, 878, 1482, 1516
SfcI CTRYAG 2 cut(s) 875, 1665
SinI GGWCC 1 cut(s) 974
Sse9I AATT 8 cut(s) 198, 272, 638, 741, 927, 1052, 1285, 1510
SsiI CCGC 5 cut(s) 50, 258, 866, 920, 1597
SspMI CTAG 5 cut(s) 474, 732, 1001, 1203, 1678
SstI GAGCTC 2 cut(s) 1326, 1536
StyD4I CCNGG 2 cut(s) 371, 1629
StyI CCWWGG 3 cut(s) 758, 1408, 1570
TaaI ACNGT 4 cut(s) 156, 299, 714, 1562
TaiI ACGT 3 cut(s) 341, 1085, 1614
TasI AATT 8 cut(s) 198, 272, 638, 741, 927, 1052, 1285, 1510
TatI WGTACW 1 cut(s) 456
TauI GCSGC 1 cut(s) 923
TfiI GAWTC 9 cut(s) 498, 541, 574, 613, 748, 754, 859, 915, 934
Tru1I TTAA 4 cut(s) 308, 528, 995, 1502
Tru9I TTAA 4 cut(s) 308, 528, 995, 1502
TscAI CASTG 4 cut(s) 232, 915, 1042, 1108
TseFI GTSAC 3 cut(s) 1291, 1584, 1607
TseI GCWGC 3 cut(s) 584, 1358, 1386
Tsp45I GTSAC 3 cut(s) 1291, 1584, 1607
TspDTI ATGAA 2 cut(s) 192, 268
TspGWI ACGGA 5 cut(s) 336, 398, 553, 773, 1603
TspRI CASTG 4 cut(s) 232, 915, 1042, 1108
Van91I CCANNNNNTGG 1 cut(s) 1244
VpaK11BI GGWCC 1 cut(s) 974
XapI RAATTY 5 cut(s) 198, 272, 638, 1052, 1510
XbaI TCTAGA 1 cut(s) 731
XmiI GTMKAC 3 cut(s) 1108, 1135, 1645
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 5 cut(s) 474, 732, 1001, 1203, 1678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.