FvH4_5g07850

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012228
Physical Location & Seq
Reverse (-)
4565792 .. 4568523
2732 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g07850.t1

Sequence Viewer

Length: 1671 bp
ATGGCGAAACCAAGAACCAGCCCCAAGTCCACACCACGAAACCCACCACCACCGCCATTCCTCCTCCACTACTTGCTCCCACTCTCTCCACTCTCCACCCTTCTCTTCTTCTCCACCAAAACGCACCGTTTCAGGCTCCTCAAACCCTCCTCCTCATCTTCATCTCCCACAATCCTCCTCCTCACTCTCATTTTCTCCCTCACTTTCTTGATAATCTCAATCCTCAACCTCCTCCCACTCCTCCCCTGTTCCCACTCTGCACCCTCCACTCCTTCCCTCTCCCACATTTCCCCGAAAGCCTCTTCGGGCTTTATCGAAGACCACAGGCTGCTTTCGGATAGCGTGATGGTGCCGCTGCCGGCACATGGGGCCTTTGGGGCTAATCTTTCCTCGGCGGAGCGGGAATTCTGGGAGCAGCCTAATGGGGAAGGGTTTAGGCCCTGTTTGGATTTCGGAATCGGGTATCGAAAAGCGTCTGGGGAGATTGTGAAGGAGAGGAGGAGGTTCTTGGTGGTGGTGGCCTCTGGGGGTTTGAATCAGCAGAGGAATCAGATTGTGGATGCTGTTGTTATTGCAAGGATTCTTGAAGCTGCTTTGGTTGTGCCTGTTTTGCAGGTCAATTTGATTTGGGGAGATGACAGTGAGTTTTCGGATATTTTCGATGTGGAACATTTCAAGAGGACTTTACAAGCTGATGTACGAGTTGTGTCGAATCTTCCCTCTACACATTTGATGTCAAGACAGACGATTATGAACAAGATCCCTCACGATGTTACTCCGCAGTGGATACATGCCAGATTCTATAATCAATTGAATCGAAATGGCCTTCTTGTGCTAAAAGGGTTGGATTCTAAACTCTCAAAGAATCTCCCCCCTGACCTTCAGAAGCTTCGGTGTAAGGTTGCTTTCCATGCATTGAGATTCGCAGCACCAATTCAAGCACTAGGGACTCAATTAGCAAGGAGAATGTGGATTGAGGGCCCTTATATTGCACTGCATCTCCGGCTAGAGAAGGATGTGTGGGTCAGAACAGGATGTCTCACTGGATTAGGCCCTGAATATGACAACATTATCACAAAGGTTCGGGAGTCTCAACCCGAATATCTTACAGGACGAGTAAACATGAGCTACACCCAGAGGCGGTTTGCTGGTCTTTGTCCCCTAAATGCTTTGGAAATAGCAAGGCTTTTGAAGGGTCTAGGTGCGCCAAGAAGTGCGCGAATATACAGTGCAGGAGGTGAGGCATTTGGGGAAAGGAGGGCTCTGCAGCCACTTGTGTCAGAATTCCCAAATTTGGTGACGAAGGAGATGTTGGCAAAAGAAGGAGAGCTCTCTCCATACATGAACAAGCCTTCAGTTCTGGCTGCCATTGACTACATTGTCTCTCTAAGTAGTGATGTCTTTCTACCTTCCCATGGCGGAAACATGGGCCGAGCAATGCAGGGTCACCGTGCTTATGTGGGACACAGAAAGTTCATAAGGCCGAACAAGAGGGCGATGATGCCGCTGTTGGAAAATACATCAATTAGTGATGATGAGTTTGGAAGCATCATGAGGGAGCTGCATAAGTCTCAGGGGAAGCCTGAGCCAAGAGGTTACATGAGAAACCGAGACGTGATTTCGAACCCTGTGCCTGAGTGCATGTGTAAACACAAAATAGGCATTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

557

Amino Acids

62.11

Weight (kDa)

9.8

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 169 - 486 2.7e-85 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63390
fragaria_vesca FvH4_5g07850
malus_domestica MD06G1145500.v1.1 MD14G1160200.v1.1
prunus_persica Prupe.5G153800_v2.0.a1
pyrus_communis pycom06g13480
rosa_chinensis RchiOBHm_Chr7g0190611
rosa_laevigata RLG00000004510
rosa_multiflora Rmu_sc0006709.1_g000010 Rmu_sc0023847.1_g000002 Rmu_sc0026450.1_g000001
rosa_roxburghii Rroxscaffold_3G00264460
rosa_rugosa Rorug06G0505600 Rorug06G0505700
rosa_samantha Rh7AG112300 Rh7BG115100 Rh7CG117200 Rh7DG116300
rosa_wichuraiana Rw7G009800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1379
Acc36I ACCTGC 1 cut(s) 604
AccB1I GGYRCC 1 cut(s) 349
AccBSI CCGCTC 1 cut(s) 400
AccII CGCG 1 cut(s) 1219
AciI CCGC 8 cut(s) 53, 353, 395, 400, 779, 1141, 1419, 1505
AclWI GGATC 1 cut(s) 754
AcsI RAATTY 3 cut(s) 404, 1283, 1291
AcuI CTGAAG 2 cut(s) 866, 1338
AfaI GTAC 1 cut(s) 699
AfiI CCNNNNNNNGG 4 cut(s) 365, 1140, 1294, 1415
AgsI TTSAA 6 cut(s) 535, 587, 676, 814, 938, 1192
AjiI CACGTC 1 cut(s) 1615
AjuI GAANNNNNNNTTGG 2 cut(s) 1295, 1327
AluBI AGCT 6 cut(s) 590, 692, 889, 1128, 1330, 1561
AluI AGCT 6 cut(s) 590, 692, 889, 1128, 1330, 1561
Alw21I GWGCWC 1 cut(s) 1332
Alw26I GTCTC 5 cut(s) 1043, 1095, 1387, 1575, 1605
AlwI GGATC 1 cut(s) 754
AoxI GGCC 8 cut(s) 369, 437, 519, 823, 979, 1051, 1429, 1481
ApaI GGGCCC 1 cut(s) 983
ApeKI GCWGC 8 cut(s) 328, 355, 415, 590, 926, 1267, 1364, 1561
ApoI RAATTY 3 cut(s) 404, 1283, 1291
AspLEI GCGC 2 cut(s) 1207, 1219
AspS9I GGNCC 6 cut(s) 369, 438, 979, 980, 1052, 1429
AsuHPI GGTGA 3 cut(s) 1250, 1309, 1439
AsuII TTCGAA 1 cut(s) 1622
BaeGI GKGCMC 1 cut(s) 983
BanI GGYRCC 1 cut(s) 349
BanII GRGCYC 3 cut(s) 983, 1264, 1332
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 1332
BbvI GCAGC 8 cut(s) 315, 342, 427, 577, 938, 1279, 1351, 1548
BccI CCATC 1 cut(s) 340
BcgI CGANNNNNNTGC 2 cut(s) 1612, 1646
BciVI GTATCC 1 cut(s) 780
BcoDI GTCTC 5 cut(s) 1043, 1095, 1387, 1575, 1605
BfaI CTAG 4 cut(s) 944, 1007, 1199, 1669
BfmI CTRYAG 1 cut(s) 1265
BfuAI ACCTGC 1 cut(s) 604
BfuI GTATCC 1 cut(s) 780
BglI GCCNNNNNGGC 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 1615
BmgT120I GGNCC 6 cut(s) 369, 438, 979, 980, 1052, 1429
BmiI GGNNCC 4 cut(s) 137, 351, 370, 981
BmsI GCATC 4 cut(s) 550, 1006, 1491, 1557
BpiI GAAGAC 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 1584
Bpu14I TTCGAA 1 cut(s) 1622
BsaJI CCNNGG 2 cut(s) 390, 1414
BsaXI ACNNNNNCTCC 2 cut(s) 984, 1014
Bsc4I CCNNNNNNNGG 4 cut(s) 365, 1140, 1294, 1415
Bse118I RCCGGY 1 cut(s) 358
Bse1I ACTGG 1 cut(s) 1048
Bse3DI GCAATG 1 cut(s) 1443
BseDI CCNNGG 2 cut(s) 390, 1414
BseGI GGATG 3 cut(s) 565, 1021, 1040
BseLI CCNNNNNNNGG 4 cut(s) 365, 1140, 1294, 1415
BseMI GCAATG 1 cut(s) 1443
BseMII CTCAG 3 cut(s) 1575, 1586, 1626
BseNI ACTGG 1 cut(s) 1048
BseSI GKGCMC 1 cut(s) 983
BseXI GCAGC 8 cut(s) 315, 342, 427, 577, 938, 1279, 1351, 1548
BsgI GTGCAG 2 cut(s) 243, 1251
Bsh1236I CGCG 1 cut(s) 1219
BshFI GGCC 8 cut(s) 371, 439, 521, 825, 981, 1053, 1431, 1483
BshNI GGYRCC 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 1332
BsiSI CCGG 2 cut(s) 359, 1003
BslFI GGGAC 3 cut(s) 961, 1143, 1476
BslI CCNNNNNNNGG 4 cut(s) 365, 1140, 1294, 1415
BsmAI GTCTC 5 cut(s) 1043, 1095, 1387, 1575, 1605
BsmBI CGTCTC 1 cut(s) 1605
BsmFI GGGAC 3 cut(s) 961, 1143, 1476
BsnI GGCC 8 cut(s) 371, 439, 521, 825, 981, 1053, 1431, 1483
Bsp119I TTCGAA 1 cut(s) 1622
Bsp120I GGGCCC 1 cut(s) 979
Bsp1286I GDGCHC 3 cut(s) 983, 1264, 1332
Bsp143I GATC 1 cut(s) 759
Bsp19I CCATGG 1 cut(s) 1414
BspACI CCGC 8 cut(s) 53, 353, 395, 400, 779, 1141, 1419, 1505
BspANI GGCC 8 cut(s) 371, 439, 521, 825, 981, 1053, 1431, 1483
BspCNI CTCAG 3 cut(s) 1576, 1585, 1627
BspFNI CGCG 1 cut(s) 1219
BspHI TCATGA 1 cut(s) 1551
BspLI GGNNCC 4 cut(s) 137, 351, 370, 981
BspMAI CTGCAG 1 cut(s) 1269
BspMI ACCTGC 1 cut(s) 604
BspPI GGATC 1 cut(s) 754
BspT104I TTCGAA 1 cut(s) 1622
BspT107I GGYRCC 1 cut(s) 349
BsrBI CCGCTC 1 cut(s) 400
BsrDI GCAATG 1 cut(s) 1443
BsrFI RCCGGY 1 cut(s) 358
BsrI ACTGG 1 cut(s) 1048
BssAI RCCGGY 1 cut(s) 358
BssECI CCNNGG 2 cut(s) 390, 1414
BssMI GATC 1 cut(s) 759
BssT1I CCWWGG 1 cut(s) 1414
Bst4CI ACNGT 4 cut(s) 128, 641, 1229, 1451
Bst6I CTCTTC 2 cut(s) 110, 307
BstBI TTCGAA 1 cut(s) 1622
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 4 cut(s) 1388, 1572, 1584, 1635
BstDSI CCRYGG 1 cut(s) 1414
BstEII GGTNACC 1 cut(s) 1445
BstF5I GGATG 3 cut(s) 565, 1021, 1040
BstFNI CGCG 1 cut(s) 1219
BstHHI GCGC 2 cut(s) 1207, 1219
BstKTI GATC 1 cut(s) 762
BstMAI GTCTC 5 cut(s) 1043, 1095, 1387, 1575, 1605
BstMBI GATC 1 cut(s) 759
BstMWI GCNNNNNNNGC 5 cut(s) 368, 377, 610, 911, 1003
BstNSI RCATGY 2 cut(s) 794, 1645
BstPI GGTNACC 1 cut(s) 1445
BstSFI CTRYAG 1 cut(s) 1265
BstSLI GKGCMC 1 cut(s) 983
BstUI CGCG 1 cut(s) 1219
BstV1I GCAGC 8 cut(s) 315, 342, 427, 577, 938, 1279, 1351, 1548
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 759
BstYI RGATCY 1 cut(s) 759
BsuI GTATCC 1 cut(s) 780
BsuRI GGCC 8 cut(s) 371, 439, 521, 825, 981, 1053, 1431, 1483
BtgI CCRYGG 1 cut(s) 1414
BtgZI GCGATG 1 cut(s) 1511
BtrI CACGTC 1 cut(s) 1615
BtsCI GGATG 3 cut(s) 565, 1021, 1040
BtsI GCAGTG 2 cut(s) 788, 992
BtsIMutI CAGTG 5 cut(s) 646, 788, 992, 1041, 1234
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 1 cut(s) 360
CciI TCATGA 1 cut(s) 1551
CfoI GCGC 2 cut(s) 1207, 1219
Cfr10I RCCGGY 1 cut(s) 358
Cfr13I GGNCC 6 cut(s) 369, 438, 979, 980, 1052, 1429
CseI GACGC 1 cut(s) 462
Csp6I GTAC 1 cut(s) 698
CviQI GTAC 1 cut(s) 698
DdeI CTNAG 4 cut(s) 1388, 1572, 1584, 1635
DpnI GATC 1 cut(s) 761
DpnII GATC 1 cut(s) 759
DrdI GACNNNNNNGTC 1 cut(s) 1379
DseDI GACNNNNNNGTC 1 cut(s) 1379
Eam1104I CTCTTC 2 cut(s) 110, 307
EarI CTCTTC 2 cut(s) 110, 307
EciI GGCGGA 2 cut(s) 410, 1434
Ecl136II GAGCTC 1 cut(s) 1330
Eco130I CCWWGG 1 cut(s) 1414
Eco24I GRGCYC 3 cut(s) 983, 1264, 1332
Eco53kI GAGCTC 1 cut(s) 1330
Eco57I CTGAAG 2 cut(s) 866, 1338
Eco91I GGTNACC 1 cut(s) 1445
EcoICRI GAGCTC 1 cut(s) 1330
EcoO109I RGGNCCY 5 cut(s) 369, 438, 979, 980, 1052
EcoO65I GGTNACC 1 cut(s) 1445
EcoRI GAATTC 2 cut(s) 404, 1283
EcoT14I CCWWGG 1 cut(s) 1414
EcoT22I ATGCAT 1 cut(s) 916
EcoT38I GRGCYC 3 cut(s) 983, 1264, 1332
ErhI CCWWGG 1 cut(s) 1414
Esp3I CGTCTC 1 cut(s) 1605
FaqI GGGAC 3 cut(s) 961, 1143, 1476
FauI CCCGC 1 cut(s) 393
FokI GGATG 3 cut(s) 572, 1028, 1047
FriOI GRGCYC 3 cut(s) 983, 1264, 1332
FspBI CTAG 4 cut(s) 944, 1007, 1199, 1669
GlaI GCGC 2 cut(s) 1206, 1218
HaeIII GGCC 8 cut(s) 371, 439, 521, 825, 981, 1053, 1431, 1483
HapII CCGG 2 cut(s) 359, 1003
HgaI GACGC 1 cut(s) 462
HhaI GCGC 2 cut(s) 1207, 1219
Hin6I GCGC 2 cut(s) 1205, 1217
HinP1I GCGC 2 cut(s) 1205, 1217
HindIII AAGCTT 1 cut(s) 887
HpaII CCGG 2 cut(s) 359, 1003
HphI GGTGA 3 cut(s) 1250, 1309, 1439
Hpy166II GTNNAC 3 cut(s) 30, 1120, 1649
Hpy188I TCNGA 7 cut(s) 337, 455, 552, 652, 885, 1028, 1282
Hpy188III TCNNGA 7 cut(s) 208, 584, 676, 738, 767, 1085, 1552
Hpy8I GTNNAC 3 cut(s) 30, 1120, 1649
HpyCH4III ACNGT 4 cut(s) 128, 641, 1229, 1451
HpyCH4IV ACGT 1 cut(s) 1614
HpyF10VI GCNNNNNNNGC 5 cut(s) 368, 377, 610, 911, 1003
HpyF3I CTNAG 4 cut(s) 1388, 1572, 1584, 1635
HpySE526I ACGT 1 cut(s) 1614
HspAI GCGC 2 cut(s) 1205, 1217
KroI GCCGGC 1 cut(s) 358
KroNI GCCGGC 1 cut(s) 360
Kzo9I GATC 1 cut(s) 759
LmnI GCTCC 5 cut(s) 81, 141, 397, 412, 1558
Lsp1109I GCAGC 8 cut(s) 315, 342, 427, 577, 938, 1279, 1351, 1548
LweI GCATC 4 cut(s) 550, 1006, 1491, 1557
MaeI CTAG 4 cut(s) 944, 1007, 1199, 1669
MaeII ACGT 1 cut(s) 1614
MaeIII GTNAC 4 cut(s) 772, 1297, 1445, 1595
MalI GATC 1 cut(s) 761
MbiI CCGCTC 1 cut(s) 400
MboI GATC 1 cut(s) 759
MboII GAAGA 6 cut(s) 97, 100, 150, 294, 329, 707
MfeI CAATTG 1 cut(s) 809
MflI RGATCY 1 cut(s) 759
MhlI GDGCHC 3 cut(s) 983, 1264, 1332
MluCI AATT 8 cut(s) 404, 619, 809, 933, 953, 1283, 1291, 1524
MlyI GAGTC 2 cut(s) 943, 1097
MmeI TCCRAC 2 cut(s) 825, 1491
Mph1103I ATGCAT 1 cut(s) 916
MroNI GCCGGC 1 cut(s) 358
MspA1I CMGCKG 2 cut(s) 355, 1507
MspI CCGG 2 cut(s) 359, 1003
MunI CAATTG 1 cut(s) 809
MvnI CGCG 1 cut(s) 1219
MwoI GCNNNNNNNGC 5 cut(s) 368, 377, 610, 911, 1003
NaeI GCCGGC 1 cut(s) 360
NcoI CCATGG 1 cut(s) 1414
NdeII GATC 1 cut(s) 759
NgoMIV GCCGGC 1 cut(s) 358
NlaIV GGNNCC 4 cut(s) 137, 351, 370, 981
NmeAIII GCCGAG 2 cut(s) 371, 1457
NmuCI GTSAC 2 cut(s) 1297, 1445
NsiI ATGCAT 1 cut(s) 916
NspI RCATGY 2 cut(s) 794, 1645
NspV TTCGAA 1 cut(s) 1622
PagI TCATGA 1 cut(s) 1551
PdiI GCCGGC 1 cut(s) 360
PleI GAGTC 2 cut(s) 943, 1096
PpsI GAGTC 2 cut(s) 943, 1096
Psp124BI GAGCTC 1 cut(s) 1332
PspEI GGTNACC 1 cut(s) 1445
PspN4I GGNNCC 4 cut(s) 137, 351, 370, 981
PspOMI GGGCCC 1 cut(s) 979
PspPI GGNCC 6 cut(s) 369, 438, 979, 980, 1052, 1429
PstI CTGCAG 1 cut(s) 1269
PsuI RGATCY 1 cut(s) 759
RsaI GTAC 1 cut(s) 699
RsaNI GTAC 1 cut(s) 698
SacI GAGCTC 1 cut(s) 1332
Sau3AI GATC 1 cut(s) 759
Sau96I GGNCC 6 cut(s) 369, 438, 979, 980, 1052, 1429
SchI GAGTC 2 cut(s) 943, 1097
SduI GDGCHC 3 cut(s) 983, 1264, 1332
SfaNI GCATC 4 cut(s) 550, 1006, 1491, 1557
SfcI CTRYAG 1 cut(s) 1265
SfuI TTCGAA 1 cut(s) 1622
Sse9I AATT 8 cut(s) 404, 619, 809, 933, 953, 1283, 1291, 1524
SsiI CCGC 8 cut(s) 53, 353, 395, 400, 779, 1141, 1419, 1505
SspMI CTAG 4 cut(s) 944, 1007, 1199, 1669
SstI GAGCTC 1 cut(s) 1332
StyI CCWWGG 1 cut(s) 1414
TaaI ACNGT 4 cut(s) 128, 641, 1229, 1451
TaiI ACGT 1 cut(s) 1617
TaqI TCGA 6 cut(s) 315, 466, 660, 710, 817, 1622
TasI AATT 8 cut(s) 404, 619, 809, 933, 953, 1283, 1291, 1524
TauI GCSGC 2 cut(s) 355, 1507
TscAI CASTG 5 cut(s) 646, 788, 999, 1048, 1234
TseFI GTSAC 2 cut(s) 1297, 1445
TseI GCWGC 8 cut(s) 328, 355, 415, 590, 926, 1267, 1364, 1561
Tsp45I GTSAC 2 cut(s) 1297, 1445
TspDTI ATGAA 4 cut(s) 150, 767, 1358, 1465
TspRI CASTG 5 cut(s) 646, 788, 999, 1048, 1234
XapI RAATTY 3 cut(s) 404, 1283, 1291
XceI RCATGY 2 cut(s) 794, 1645
XspI CTAG 4 cut(s) 944, 1007, 1199, 1669
Zsp2I ATGCAT 1 cut(s) 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.