Rw7G009800

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012228
Physical Location & Seq
Forward (+)
9084347 .. 9086896
2550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009800.1

Sequence Viewer

Length: 1698 bp
ATGGCGAAATCAAGAACGAGCCCCAAGACAACATCACGAAACCCATCACCATCACCACCGCCAATATTCCTCTTCCACTACTTGCTCCCAGTCTCTGCACTCACCACCCTCCTCTTCTCCCCCAAAACGCACCGTTTCAGACTCCCCAAACCCTCCTCCTCCTCCTCATCATCATCTCCCTCAATCCTCCTTCTCTGTTTCCTCTTCTCCCTCACTTTCTTGGGAATCTCACTCCTCAATCTCATCCCCACCTACTCTGCCATCATCCCCTGTTCCCACTCTGCTGCCTCCACTCCTTCCCTCTCCCCCATTTCCCCGAAAACCTCTTCTTCGGGCTTTATCGACGACCCGAGGCTGCTTTCGGATACTGTCATGGTGCCACTGCCGGCCCATGGGGCCTTGGGGGCTAATCTTTCCTCGGAGGAGAAGGAATTCTGGGAGCAGCCCAATGGGGAAGGGTTTAGGCCCTGCTTGGATTTCGGCCTCGGGTATCGAATATCGTCTTCCAAGATTGTGAAAGAGAGGAGGAGGTTCTTGCTGGTGGTGGCCTCTGGGGGTCTGAACCAGCAGAGGAATCAGATTGTGGATGCTGTTGTCATTGCTAGGGTTCTTGAGGCTGTTTTGGTTGTGCCTGTTTTGCAGGTCAATTTGATCTGGGGAGACGAAAGTGAGTTTTCAGATATTTTCGATGTGGCACACTTCAAGAGGACTTTACAAGCTGATGTACGAGTTGTGTCAAATCTGCCCTCTACACATTTGATGTCAAGACAGAAAATTATGAACAAGATCCCTCACGATGTTACTCCGCAGTGGATACATGCCAGATTCTATAATCAACTGAATCAAGATGGCCTTCTTGTGCTAAAAGGGCTGGATTCTAAGCTCTCAAAGAATCTCCCCCCTGATCTTCAGAAGCTTCGGTGTAAGGTTGCTTTCCATTCATTGAGATTTGCAGCGCCAATTCAAGCACTAGGGAATCAATTAGCAAAGAGAATGTGGATTGAAGGGCCTTACATTGCACTCCATCTCCGGCTAGAGAAGGATGTGTGGGTCAGAACAGGATGTCTCACCGGATTAGGCCCTGAATATGATGACATTATCACAAAGGTTCGGGAGTCTCAACCCGAATATCTTACAGGACGATTGAACATGACCTACAACCAAAGGAGACTTGCTGGTCTCTGTCCCCTAAATGCATTAGAAATAGCAAGGCTTTTGAAGGGTCTAGGAGCGCCAAGAAGTGCACGGATATATAGTGCAGGAGGTGAGGCATTTGGGGAAAGCAGGGCTCTGCAGCCACTTGTGGCAGAATTTCCAAATTTGGTGACAAAGGAGATGTTGGCGAAAGAAGGAGAGCTCTCTCCATACATGAACAAGCATTCAGCTCTGGCTGCCATTGACTACCTTGTCTCTCTAAGCAGTGATGTCTTTGTGCCTTCCCATGGTGGAAACATGGGCCGAGCAATGCAGGGTCACCGTGCTTATGTGGGACATAGAAAGTTCATAAAGCCGAACAAGAGAGCAATGATGCCTCTGTTTGAAGATACATTGATTAGTGATGATGAGTTCGGAAGCATCATGAGGGAGCTGCATAAGTCTCAGGGGAAGCCTGAGCCAAGAGGTTACATGAGAAACCGAGATGTGATTTCGCACCCTGTGCCTGAGTGCATGTGTAAACACAAAACAGGCATTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

565

Amino Acids

62.78

Weight (kDa)

9.57

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 178 - 495 6.6e-87 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63390
fragaria_vesca FvH4_5g07850
malus_domestica MD06G1145500.v1.1 MD14G1160200.v1.1
prunus_persica Prupe.5G153800_v2.0.a1
pyrus_communis pycom06g13480
rosa_chinensis RchiOBHm_Chr7g0190611
rosa_laevigata RLG00000004510
rosa_multiflora Rmu_sc0006709.1_g000010 Rmu_sc0023847.1_g000002 Rmu_sc0026450.1_g000001
rosa_roxburghii Rroxscaffold_3G00264460
rosa_rugosa Rorug06G0505600 Rorug06G0505700
rosa_samantha Rh7AG112300 Rh7BG115100 Rh7CG117200 Rh7DG116300
rosa_wichuraiana Rw7G009800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1176, 1406
Acc36I ACCTGC 1 cut(s) 631
AccB1I GGYRCC 1 cut(s) 376
AciI CCGC 2 cut(s) 59, 806
AclWI GGATC 1 cut(s) 781
AcsI RAATTY 3 cut(s) 431, 1310, 1318
AcuI CTGAAG 1 cut(s) 893
AdeI CACNNNGTG 1 cut(s) 1657
AfaI GTAC 1 cut(s) 726
AfiI CCNNNNNNNGG 2 cut(s) 392, 1442
AgsI TTSAA 6 cut(s) 703, 965, 1004, 1147, 1219, 1541
AluBI AGCT 6 cut(s) 719, 883, 916, 1357, 1385, 1588
AluI AGCT 6 cut(s) 719, 883, 916, 1357, 1385, 1588
Alw21I GWGCWC 2 cut(s) 1246, 1359
Alw26I GTCTC 8 cut(s) 97, 654, 1070, 1122, 1162, 1184, 1414, 1602
Alw44I GTGCAC 1 cut(s) 1242
AlwI GGATC 1 cut(s) 781
AlwNI CAGNNNCTG 1 cut(s) 95
Ama87I CYCGRG 2 cut(s) 349, 485
AoxI GGCC 9 cut(s) 387, 396, 464, 481, 546, 850, 1007, 1078, 1456
ApaLI GTGCAC 1 cut(s) 1242
ApeKI GCWGC 7 cut(s) 284, 355, 442, 953, 1294, 1391, 1588
ApoI RAATTY 3 cut(s) 431, 1310, 1318
Asp700I GAANNNNTTC 1 cut(s) 431
AspLEI GCGC 2 cut(s) 958, 1234
AspS9I GGNCC 6 cut(s) 388, 396, 465, 1007, 1079, 1456
AsuHPI GGTGA 7 cut(s) 39, 45, 94, 1060, 1277, 1336, 1466
AvaI CYCGRG 2 cut(s) 349, 485
BaeGI GKGCMC 1 cut(s) 1246
BanI GGYRCC 1 cut(s) 376
BanII GRGCYC 3 cut(s) 23, 1291, 1359
BbsI GAAGAC 1 cut(s) 495
Bbv12I GWGCWC 2 cut(s) 1246, 1359
BbvI GCAGC 7 cut(s) 271, 342, 454, 965, 1306, 1378, 1575
BccI CCATC 5 cut(s) 52, 58, 269, 842, 1032
BciVI GTATCC 2 cut(s) 358, 807
BcoDI GTCTC 8 cut(s) 97, 654, 1070, 1122, 1162, 1184, 1414, 1602
BfaI CTAG 5 cut(s) 603, 971, 1034, 1226, 1696
BfmI CTRYAG 1 cut(s) 1292
BfoI RGCGCY 2 cut(s) 959, 1235
BfuAI ACCTGC 1 cut(s) 631
BfuI GTATCC 2 cut(s) 358, 807
BglI GCCNNNNNGGC 2 cut(s) 395, 404
BisI GCNGC 7 cut(s) 285, 356, 443, 954, 1295, 1392, 1589
BlsI GCNGC 7 cut(s) 286, 357, 444, 955, 1296, 1393, 1590
BmeT110I CYCGRG 2 cut(s) 349, 485
BmgT120I GGNCC 6 cut(s) 388, 396, 465, 1007, 1079, 1456
BmiI GGNNCC 2 cut(s) 378, 397
BmrI ACTGGG 1 cut(s) 83
BmsI GCATC 3 cut(s) 577, 1518, 1584
BmuI ACTGGG 1 cut(s) 83
BpiI GAAGAC 1 cut(s) 495
Bpu10I CCTNAGC 1 cut(s) 1611
BpuEI CTTGAG 1 cut(s) 632
BsaI GGTCTC 1 cut(s) 1184
BsaJI CCNNGG 6 cut(s) 350, 391, 399, 417, 484, 1441
BsaWI WCCGGW 1 cut(s) 1070
BsaXI ACNNNNNCTCC 2 cut(s) 1011, 1041
Bsc4I CCNNNNNNNGG 2 cut(s) 392, 1442
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 1 cut(s) 89
Bse3DI GCAATG 4 cut(s) 597, 1014, 1470, 1530
BseDI CCNNGG 6 cut(s) 350, 391, 399, 417, 484, 1441
BseGI GGATG 5 cut(s) 243, 264, 592, 1048, 1067
BseLI CCNNNNNNNGG 2 cut(s) 392, 1442
BseMI GCAATG 4 cut(s) 597, 1014, 1470, 1530
BseMII CTCAG 3 cut(s) 1602, 1613, 1653
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 9 cut(s) 101, 145, 148, 151, 154, 224, 437, 538, 541
BseSI GKGCMC 1 cut(s) 1246
BseXI GCAGC 7 cut(s) 271, 342, 454, 965, 1306, 1378, 1575
BsgI GTGCAG 2 cut(s) 81, 1278
BshFI GGCC 9 cut(s) 389, 398, 466, 483, 548, 852, 1009, 1080, 1458
BshNI GGYRCC 1 cut(s) 376
BsiHKAI GWGCWC 2 cut(s) 1246, 1359
BsiHKCI CYCGRG 2 cut(s) 349, 485
BsiSI CCGG 3 cut(s) 386, 1030, 1071
BslFI GGGAC 2 cut(s) 1170, 1503
BslI CCNNNNNNNGG 2 cut(s) 392, 1442
BsmAI GTCTC 8 cut(s) 97, 654, 1070, 1122, 1162, 1184, 1414, 1602
BsmBI CGTCTC 1 cut(s) 654
BsmFI GGGAC 2 cut(s) 1170, 1503
BsmI GAATGC 1 cut(s) 1378
BsnI GGCC 9 cut(s) 389, 398, 466, 483, 548, 852, 1009, 1080, 1458
Bso31I GGTCTC 1 cut(s) 1184
BsoBI CYCGRG 2 cut(s) 349, 485
Bsp1286I GDGCHC 4 cut(s) 23, 1246, 1291, 1359
Bsp143I GATC 3 cut(s) 651, 786, 904
Bsp19I CCATGG 2 cut(s) 391, 1441
BspACI CCGC 2 cut(s) 59, 806
BspANI GGCC 9 cut(s) 389, 398, 466, 483, 548, 852, 1009, 1080, 1458
BspCNI CTCAG 3 cut(s) 1603, 1612, 1654
BspHI TCATGA 1 cut(s) 1578
BspLI GGNNCC 2 cut(s) 378, 397
BspMAI CTGCAG 1 cut(s) 1296
BspMI ACCTGC 1 cut(s) 631
BspPI GGATC 1 cut(s) 781
BspT107I GGYRCC 1 cut(s) 376
BspTNI GGTCTC 1 cut(s) 1184
BsrDI GCAATG 4 cut(s) 597, 1014, 1470, 1530
BsrFI RCCGGY 1 cut(s) 385
BsrI ACTGG 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 6 cut(s) 350, 391, 399, 417, 484, 1441
BssMI GATC 3 cut(s) 651, 786, 904
BssT1I CCWWGG 3 cut(s) 391, 399, 1441
Bst4CI ACNGT 3 cut(s) 134, 370, 1478
Bst6I CTCTTC 4 cut(s) 77, 119, 209, 331
BstAPI GCANNNNNTGC 1 cut(s) 1657
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 5 cut(s) 879, 1415, 1599, 1611, 1662
BstDSI CCRYGG 2 cut(s) 391, 1441
BstEII GGTNACC 1 cut(s) 1472
BstF5I GGATG 5 cut(s) 243, 264, 592, 1048, 1067
BstH2I RGCGCY 2 cut(s) 959, 1235
BstHHI GCGC 2 cut(s) 958, 1234
BstKTI GATC 3 cut(s) 654, 789, 907
BstMAI GTCTC 8 cut(s) 97, 654, 1070, 1122, 1162, 1184, 1414, 1602
BstMBI GATC 3 cut(s) 651, 786, 904
BstMWI GCNNNNNNNGC 6 cut(s) 395, 404, 637, 868, 1391, 1657
BstNSI RCATGY 2 cut(s) 821, 1672
BstPI GGTNACC 1 cut(s) 1472
BstSFI CTRYAG 1 cut(s) 1292
BstSLI GKGCMC 1 cut(s) 1246
BstV1I GCAGC 7 cut(s) 271, 342, 454, 965, 1306, 1378, 1575
BstV2I GAAGAC 1 cut(s) 495
BstX2I RGATCY 1 cut(s) 786
BstYI RGATCY 1 cut(s) 786
BsuI GTATCC 2 cut(s) 358, 807
BsuRI GGCC 9 cut(s) 389, 398, 466, 483, 548, 852, 1009, 1080, 1458
BtgI CCRYGG 2 cut(s) 391, 1441
BtsCI GGATG 5 cut(s) 243, 264, 592, 1048, 1067
BtsI GCAGTG 3 cut(s) 380, 815, 1426
BtsIMutI CAGTG 3 cut(s) 380, 815, 1426
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 1 cut(s) 387
CaiI CAGNNNCTG 1 cut(s) 95
CciI TCATGA 1 cut(s) 1578
CfoI GCGC 2 cut(s) 958, 1234
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 6 cut(s) 388, 396, 465, 1007, 1079, 1456
Csp6I GTAC 1 cut(s) 725
CviQI GTAC 1 cut(s) 725
DdeI CTNAG 5 cut(s) 879, 1415, 1599, 1611, 1662
DpnI GATC 3 cut(s) 653, 788, 906
DpnII GATC 3 cut(s) 651, 786, 904
DraIII CACNNNGTG 1 cut(s) 1657
DrdI GACNNNNNNGTC 2 cut(s) 1176, 1406
DseDI GACNNNNNNGTC 2 cut(s) 1176, 1406
Eam1104I CTCTTC 4 cut(s) 77, 119, 209, 331
EarI CTCTTC 4 cut(s) 77, 119, 209, 331
Ecl136II GAGCTC 1 cut(s) 1357
Eco130I CCWWGG 3 cut(s) 391, 399, 1441
Eco24I GRGCYC 3 cut(s) 23, 1291, 1359
Eco31I GGTCTC 1 cut(s) 1184
Eco53kI GAGCTC 1 cut(s) 1357
Eco57I CTGAAG 1 cut(s) 893
Eco88I CYCGRG 2 cut(s) 349, 485
Eco91I GGTNACC 1 cut(s) 1472
EcoICRI GAGCTC 1 cut(s) 1357
EcoO109I RGGNCCY 4 cut(s) 396, 465, 1007, 1079
EcoO65I GGTNACC 1 cut(s) 1472
EcoRI GAATTC 1 cut(s) 431
EcoT14I CCWWGG 3 cut(s) 391, 399, 1441
EcoT22I ATGCAT 1 cut(s) 1198
EcoT38I GRGCYC 3 cut(s) 23, 1291, 1359
ErhI CCWWGG 3 cut(s) 391, 399, 1441
Esp3I CGTCTC 1 cut(s) 654
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FaqI GGGAC 2 cut(s) 1170, 1503
Fnu4HI GCNGC 7 cut(s) 285, 356, 443, 954, 1295, 1392, 1589
FokI GGATG 5 cut(s) 230, 251, 599, 1055, 1074
FriOI GRGCYC 3 cut(s) 23, 1291, 1359
Fsp4HI GCNGC 7 cut(s) 285, 356, 443, 954, 1295, 1392, 1589
FspBI CTAG 5 cut(s) 603, 971, 1034, 1226, 1696
GlaI GCGC 2 cut(s) 957, 1233
GluI GCNGC 7 cut(s) 285, 356, 443, 954, 1295, 1392, 1589
HaeII RGCGCY 2 cut(s) 959, 1235
HaeIII GGCC 9 cut(s) 389, 398, 466, 483, 548, 852, 1009, 1080, 1458
HapII CCGG 3 cut(s) 386, 1030, 1071
HhaI GCGC 2 cut(s) 958, 1234
Hin6I GCGC 2 cut(s) 956, 1232
HinP1I GCGC 2 cut(s) 956, 1232
HindIII AAGCTT 1 cut(s) 914
HinfI GANTC 9 cut(s) 141, 225, 574, 825, 841, 875, 892, 976, 1115
HpaII CCGG 3 cut(s) 386, 1030, 1071
HphI GGTGA 7 cut(s) 39, 45, 94, 1060, 1277, 1336, 1466
Hpy166II GTNNAC 2 cut(s) 1244, 1676
Hpy188I TCNGA 9 cut(s) 140, 364, 421, 561, 579, 679, 912, 1055, 1571
Hpy188III TCNNGA 9 cut(s) 12, 36, 611, 703, 765, 794, 845, 1112, 1579
Hpy8I GTNNAC 2 cut(s) 1244, 1676
Hpy99I CGWCG 1 cut(s) 347
HpyCH4III ACNGT 3 cut(s) 134, 370, 1478
HpyF10VI GCNNNNNNNGC 6 cut(s) 395, 404, 637, 868, 1391, 1657
HpyF3I CTNAG 5 cut(s) 879, 1415, 1599, 1611, 1662
HspAI GCGC 2 cut(s) 956, 1232
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Kzo9I GATC 3 cut(s) 651, 786, 904
LmnI GCTCC 4 cut(s) 90, 439, 1229, 1585
Lsp1109I GCAGC 7 cut(s) 271, 342, 454, 965, 1306, 1378, 1575
LweI GCATC 3 cut(s) 577, 1518, 1584
MaeI CTAG 5 cut(s) 603, 971, 1034, 1226, 1696
MaeIII GTNAC 4 cut(s) 799, 1324, 1472, 1622
MalI GATC 3 cut(s) 653, 788, 906
MboI GATC 3 cut(s) 651, 786, 904
MboII GAAGA 8 cut(s) 64, 106, 196, 318, 321, 495, 899, 1553
MflI RGATCY 1 cut(s) 786
MhlI GDGCHC 4 cut(s) 23, 1246, 1291, 1359
MluCI AATT 7 cut(s) 431, 646, 774, 960, 980, 1310, 1318
MlyI GAGTC 2 cut(s) 135, 1124
Mph1103I ATGCAT 1 cut(s) 1198
MroNI GCCGGC 1 cut(s) 385
MroXI GAANNNNTTC 1 cut(s) 431
MspI CCGG 3 cut(s) 386, 1030, 1071
Mva1269I GAATGC 1 cut(s) 1378
MwoI GCNNNNNNNGC 6 cut(s) 395, 404, 637, 868, 1391, 1657
NaeI GCCGGC 1 cut(s) 387
NcoI CCATGG 2 cut(s) 391, 1441
NdeII GATC 3 cut(s) 651, 786, 904
NgoMIV GCCGGC 1 cut(s) 385
NlaIV GGNNCC 2 cut(s) 378, 397
NmeAIII GCCGAG 1 cut(s) 1484
NmuCI GTSAC 2 cut(s) 1324, 1472
NsiI ATGCAT 1 cut(s) 1198
NspI RCATGY 2 cut(s) 821, 1672
PagI TCATGA 1 cut(s) 1578
PctI GAATGC 1 cut(s) 1378
PdiI GCCGGC 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 431
PfeI GAWTC 7 cut(s) 225, 574, 825, 841, 875, 892, 976
PkrI GCNGC 7 cut(s) 286, 357, 444, 955, 1296, 1393, 1590
PleI GAGTC 2 cut(s) 135, 1123
PpsI GAGTC 2 cut(s) 135, 1123
Psp124BI GAGCTC 1 cut(s) 1359
PspEI GGTNACC 1 cut(s) 1472
PspN4I GGNNCC 2 cut(s) 378, 397
PspPI GGNCC 6 cut(s) 388, 396, 465, 1007, 1079, 1456
PsrI GAACNNNNNNTAC 2 cut(s) 1139, 1171
PstI CTGCAG 1 cut(s) 1296
PstNI CAGNNNCTG 1 cut(s) 95
PsuI RGATCY 1 cut(s) 786
RsaI GTAC 1 cut(s) 726
RsaNI GTAC 1 cut(s) 725
SacI GAGCTC 1 cut(s) 1359
SatI GCNGC 7 cut(s) 285, 356, 443, 954, 1295, 1392, 1589
Sau3AI GATC 3 cut(s) 651, 786, 904
Sau96I GGNCC 6 cut(s) 388, 396, 465, 1007, 1079, 1456
SchI GAGTC 2 cut(s) 135, 1124
SduI GDGCHC 4 cut(s) 23, 1246, 1291, 1359
SfaNI GCATC 3 cut(s) 577, 1518, 1584
SfcI CTRYAG 1 cut(s) 1292
SfiI GGCCNNNNNGGCC 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 7 cut(s) 431, 646, 774, 960, 980, 1310, 1318
SsiI CCGC 2 cut(s) 59, 806
SspI AATATT 1 cut(s) 66
SspMI CTAG 5 cut(s) 603, 971, 1034, 1226, 1696
SstI GAGCTC 1 cut(s) 1359
StyI CCWWGG 3 cut(s) 391, 399, 1441
TaaI ACNGT 3 cut(s) 134, 370, 1478
TaqI TCGA 3 cut(s) 342, 493, 687
TasI AATT 7 cut(s) 431, 646, 774, 960, 980, 1310, 1318
TfiI GAWTC 7 cut(s) 225, 574, 825, 841, 875, 892, 976
TscAI CASTG 3 cut(s) 387, 815, 1426
TseFI GTSAC 2 cut(s) 1324, 1472
TseI GCWGC 7 cut(s) 284, 355, 442, 953, 1294, 1391, 1588
Tsp45I GTSAC 2 cut(s) 1324, 1472
TspDTI ATGAA 4 cut(s) 794, 930, 1385, 1492
TspGWI ACGGA 1 cut(s) 1261
TspRI CASTG 3 cut(s) 387, 815, 1426
VneI GTGCAC 1 cut(s) 1242
XapI RAATTY 3 cut(s) 431, 1310, 1318
XceI RCATGY 2 cut(s) 821, 1672
XmnI GAANNNNTTC 1 cut(s) 431
XspI CTAG 5 cut(s) 603, 971, 1034, 1226, 1696
Zsp2I ATGCAT 1 cut(s) 1198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.