Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007117

Physical Location & Seq

Genomic Coordinates Forward (+)

25599514 .. 25606841

Gene Structure 7328 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G63960.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3288 bp

ATGAATAGATCCGGTATTTCCAAAAAGCGACCGCCTCCTTCGAATACCCCACCACCGGCGGGTAAGCATCGAGCCACTGGTGATTCAACACCATCTCCGGCCATCGGAACCCTAGATGATGAATTTATGATGGAAGAGGACGTGTTTCTGGACGAAACTCTCTTGTACGGCGACGAAGATGAGGAATCCCTAATCCTCCGTGACATTGAGGAGCGTGAATCGCGTTCCTCGGCTTGGGCTCGACCTCCGCTCTCCCCCGCGTATCTCTCGAATTCACAGAGTATCATTTTCCAACAATTGGAGATTGACTCTATAATCGCGGAGAGTCATAAGGAGCTGTTACCGGGTTCCTCAGGGCAAGCTCCAATCATTAGGATGTTTGGGGTTACCAGAGAAGGTAACAGTGTGTGTTGCTTTGTTCATGGATTTGAGCCATACTTTTACATTGCTTGCCCTCCTGGAATGGGGCCAGACGATATTTCTAATTTCCATCAGAGTCTTGAGGGAAGGATGAGGGAATCCAATAAAAATGCCAAGGTCCCGAAATTTGTTAAACGTATAGAAATGGTGCAGAAAAGAAGCATTATGTATTACCAACAGCAAAAATCCCAAACTTTTCTGAAGATTACAGTTGCATTGCCGACTATGGTGGCAAGCTGTCGCGGCATCCTTGATAGAGGCCTACAAATTGATGGATTGGGTATGAAGAGCTTCCAGACATATGAAAGCAATATTCTTTTCGTTCTCCGTTTCATGGTTGATTGTGATATTGTCGGAGGAAATTGGATTGAAGTACCTACTGGGAAGTATAAGAAAAATGCAAGAACTTTGTCATACTGCCAATTGGAGTTCCATTGCCTGTACTCAGATCTAATCAGTCATGCTGCAGAAGGTGAATACTCAAAAATGGCTCCATTCCGTGTACTAAGTTTCGATATTGAGTGTGCAGGTCGTAAAGGACATTTTCCGGAAGCTAAGCATGATCCTGTAATCCAGATAGCGAACCTTGTTACTCTTCAGGGAGAGGATCACCCATTTGTACGCAATGTCATGACTCTTAAGTCATGTGCTCCAATCGTAGGCGTAGATGTCATGTCTTTTGAAACAGAAAGAGAGGTCTTACTAGCTTGGAGGGATTTGATTCGTGATGTTGATCCTGATATCATCATTGGTTATAACATCTGCAAATTCGATTTACCTTATCTGATTGAGAGAGCTGCTACACTGGGAATAGAGGAATTTCCTCTTCTTGGTCGTGTAAAGAACAGTAGGGTCCGGGTCAGGGACTCAACATTTTCATCAAGACAACAAGGAATAAGAGAAAGTAAAGAGACCACAATTGAAGGAAGATTTCAGTTTGACCTTATTCAGGCAATACACAGAGACCACAAATTAAGTTCTTATTCGCTGAATTCTGTCTCAGCTCACTTTCTTTCCGAGCAGAAAGAAGATGTCCACCATTCTATAATAACTGATCTCCAGAATGGGAATGCGGAAACCAGGAGGCGTCTTGCTGTTTATTGTTTGAAGGATGCATATCTTCCTCAGAGGCTTCTGGACAAACTGATGTTTATATATAATTATGTCGAAATGGCTCGTGTAACTGGTGTCCCTATTTCATTTCTTCTTGCGAGAGGACAGAGTATCAAGGTTTTATCTCAGCTTCTTAGGAAAGGCAAACAGAAAAATCTGGTTCTTCCAAATGCTAAACAGTCAGGGTCCGAACAAGGAACTTATGAAGGCGCAACTGTTTTAGAAGCAAGAACAGGTTTCTATGAAAAGCCAATTGCAACTTTGGATTTTGCTTCACTGTACCCGTCAATTATGATGGCATATAATCTGTGCTACTGCACCTTGGTGACACCTGAAGATGTACGCAAACTGAATCTTCCACCTGAACATGTCACTAAAACTCCATCAGGGGAAACATTTGTTAAGCAAACTTTGCAAAAGGGTATACTTCCAGAAATTCTCGAAGAGCTTCTTACTGCCCGTAAGAGAGCTAAAGCAGATTTAAAGGAGGCTAAGGATCCCCTTGAGAAGGCTGTTTTAGATGGTAGACAGTTAGCGTTGAAGATCAGTGCAAATTCTGTCTACGGGTTTACGGGAGCCACTGTTGGGCAGTTACCATGCTTAGAAATATCCTCGAGTGTAACTAGCTATGGTCGTCAGATGATTGAACAAACAAAGAAACTTGTTGAAGACAAATTCACAACACTGGGAGGGTATCAATACAATGCAGAGGTCATTTATGGAGACACGGATTCAGTCATGGTGCAATTTGGAGTATCGGATGTAGAAGCTGCGATGACCTTGGGGAGGGAAGCTGCAGAACACATTAGTGGAACTTTTATCAAACCCATCAAATTGGAGTTTGAAAAGGTCTATTTCCCATATCTTCTCATTAACAAGAAGAGGTATGCTGGTTTGCTATGGACAAATCCTCAACAGTTTGACAAAATGGACACCAAAGGAATCGAGACAGTACGAAGGGATAATTGTTTACTGGTTAAGAACCTCGTGACTGAGAGTCTTAACAAAATACTTATTGATAGAGATGTTCCAGGGGCAGCTGAAAATGTCAAGAAAACCATTTCGGATCTTCTCATGAACCGTATTGACTTGTCACTTTTGGTGATTACTAAGGGTCTAACGAAAACAGGAGATGATTATGAAGTTAAATCAGCTCATGGTGAACTTGCTGAACGCATGCGTAAGAGGGATGCTGCTACAGCGCCAAATGTTGGAGATCGAGTACCGTATGTTATCATAAAAGCTGCTAAAGGTGCCAAGGCTTATGAACGATCAGAAGATCCAATCTACGTGCTACAGAATAATATCCCTATAGACCCAAATTACTACTTGGAGAATCAGATTAGCAAGCCACTTCTTAGGATTTTTGAGCCAGTCCTGAAAAATGCTAGCAAGGAGCTTCTCCATGGAAGTCACACGAGGTCAATATCAATCACTACTCCTTCAAACAGCGGTATAATGAAGTTTGCTAAAAAACAACTGAGCTGTGTTGGCTGCAAAGTTCCGATCAGCAATGGAACACTATGCGCAAGTTGCAAGGGAAGAGAAGCCGAGTTATATTGCAAAAACGTGTCTCAAGTGGCTGAGCTTGAAGAGGTTTTTGGGAGGCTGTGGACACAGTGCCAGGAGTGTCAAGGCTCTCTTCATCAAGATGTCTTGTGCACCAGTCGAGATTGTCCAATATTTTACCGGAGAATGAAAGCACAAAAAGACATGGCTGTAGCCAGGCAACAACTCGACCGTTGGAGCTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008296 GO:0008408 GO:0009058 GO:0009059 GO:0009987 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0030894 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0033554 GO:0034641 GO:0034645 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0043625 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0140097 GO:1901360 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko03430 ko03440 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map03430 map03440 map05166

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 zf-C4pol

Protein Analysis

1095

Amino Acids

123.23

Weight (kDa)

8.0

Isoelectric Point (pI)

46.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
POL3_N	PF24055	142 - 217	2.8e-08	DNA polymerase delta catalytic subunit-like, N-terminal domain
DNA_pol_B_exo1	PF03104	239 - 378	1.3e-35	DNA polymerase family B, exonuclease domain
DNA_pol_B_exo1	PF03104	396 - 490	1.6e-23	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	554 - 987	4.9e-160	DNA polymerase family B
zf-C4pol	PF14260	1024 - 1093	2.2e-18	C4-type zinc-finger of DNA polymerase delta

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0007117)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63960 AT5G63960
fragaria_vesca	FvH4_1g24660 FvH4_1g24660 FvH4_1g24660 FvH4_1g24660 FvH4_1g24660
malus_domestica	MD00G1053300.v1.1 MD05G1053800.v1.1 MD07G1029900.v1.1
prunus_persica	Prupe.4G273600_v2.0.a1 Prupe.4G273600_v2.0.a1 Prupe.4G273600_v2.0.a1
pyrus_communis	pycom01g00060 pycom07g02190
rosa_chinensis	RchiOBHm_Chr2g0122111
rosa_laevigata	RLG00000018650
rosa_multiflora	Rmu_co8081762.1_g000001
rosa_roxburghii	Rroxscaffold_2G00122010
rosa_rugosa	Rorug02G0236500
rosa_samantha	Rh2AG293000 Rh2BG303400 Rh2CG281900 Rh2DG317000
rosa_wichuraiana	Rw2G023500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1176
AasI	GACNNNNNNGTC	1 cut(s)	1060
Acc16I	TGCGCA	1 cut(s)	3061
Acc36I	ACCTGC	1 cut(s)	938
AccB1I	GGYRCC	1 cut(s)	2786
AccB7I	CCANNNNNTGG	2 cut(s)	298, 2744
AccBSI	CCGCTC	1 cut(s)	250
AccI	GTMKAC	3 cut(s)	1957, 2059, 2094
AccII	CGCG	4 cut(s)	223, 260, 320, 663
AccIII	TCCGGA	1 cut(s)	967
AciI	CCGC	8 cut(s)	32, 59, 248, 258, 320, 663, 1493, 2985
AclWI	GGATC	8 cut(s)	3, 977, 1035, 1148, 2024, 2037, 2607, 2807
AcoI	YGGCCR	1 cut(s)	99
AcsI	RAATTY	9 cut(s)	122, 271, 545, 1187, 1238, 1411, 1968, 2086, 2207
AcuI	CTGAAG	3 cut(s)	641, 1001, 1887
AcyI	GRCGYC	1 cut(s)	1507
AfaI	GTAC	9 cut(s)	167, 795, 863, 924, 1041, 1814, 1875, 2487, 2757
AflII	CTTAAG	1 cut(s)	1058
AflIII	ACRYGT	3 cut(s)	141, 1900, 3102
AhdI	GACNNNNNGTC	1 cut(s)	2529
AjiI	CACGTC	1 cut(s)	142
AjnI	CCWGG	5 cut(s)	457, 1499, 2563, 3156, 3257
AjuI	GAANNNNNNNTTGG	2 cut(s)	3117, 3149
AleI	CACNNNNGTG	2 cut(s)	1856, 2340
Alw21I	GWGCWC	2 cut(s)	1072, 3197
Alw26I	GTCTC	6 cut(s)	1325, 1377, 1423, 2250, 2474, 3111
Alw44I	GTGCAC	1 cut(s)	3193
AlwI	GGATC	8 cut(s)	3, 977, 1035, 1148, 2024, 2037, 2607, 2807
Ama87I	CYCGRG	1 cut(s)	2146
Aor13HI	TCCGGA	1 cut(s)	967
AoxI	GGCC	3 cut(s)	99, 467, 679
ApaLI	GTGCAC	1 cut(s)	3193
ApeKI	GCWGC	8 cut(s)	884, 1217, 2303, 2327, 2570, 2726, 2777, 3027
ApoI	RAATTY	9 cut(s)	122, 271, 545, 1187, 1238, 1411, 1968, 2086, 2207
Asp700I	GAANNNNTTC	2 cut(s)	710, 1980
AspLEI	GCGC	3 cut(s)	1745, 2737, 3062
AspS9I	GGNCC	4 cut(s)	467, 538, 1273, 1719
AsuC2I	CCSGG	2 cut(s)	345, 1277
AsuHPI	GGTGA	6 cut(s)	92, 905, 1022, 1870, 2647, 2705
AsuII	TTCGAA	1 cut(s)	41
AsuNHI	GCTAGC	1 cut(s)	2921
AvaI	CYCGRG	1 cut(s)	2146
AvaII	GGWCC	3 cut(s)	538, 1273, 1719
AxyI	CCTNAGG	1 cut(s)	352
BaeGI	GKGCMC	1 cut(s)	3197
BamHI	GGATCC	1 cut(s)	2029
BanI	GGYRCC	1 cut(s)	2786
BanII	GRGCYC	1 cut(s)	241
BauI	CACGAG	3 cut(s)	1596, 2519, 2950
BbsI	GAAGAC	1 cut(s)	2208
Bbv12I	GWGCWC	2 cut(s)	1072, 3197
BbvI	GCAGC	8 cut(s)	871, 1204, 2290, 2314, 2582, 2713, 2764, 3014
BccI	CCATC	9 cut(s)	100, 110, 124, 498, 686, 1822, 1924, 2048, 2369
BceAI	ACGGC	1 cut(s)	184
BciT130I	CCWGG	5 cut(s)	459, 1501, 2565, 3158, 3259
BcnI	CCSGG	2 cut(s)	345, 1277
BcoDI	GTCTC	6 cut(s)	1325, 1377, 1423, 2250, 2474, 3111
BfaI	CTAG	4 cut(s)	113, 1124, 2157, 2922
BfmI	CTRYAG	6 cut(s)	885, 2328, 2730, 2828, 2844, 3252
BfoI	RGCGCY	1 cut(s)	2738
BfrI	CTTAAG	1 cut(s)	1058
BfuAI	ACCTGC	1 cut(s)	938
BglII	AGATCT	1 cut(s)	868
BisI	GCNGC	9 cut(s)	664, 885, 1218, 2304, 2328, 2571, 2727, 2778, 3028
BlpI	GCTNAGC	2 cut(s)	975, 3117
BlsI	GCNGC	9 cut(s)	665, 886, 1219, 2305, 2329, 2572, 2728, 2779, 3029
Bme1390I	CCNGG	7 cut(s)	345, 459, 1277, 1501, 2565, 3158, 3259
Bme18I	GGWCC	3 cut(s)	538, 1273, 1719
BmeRI	GACNNNNNGTC	1 cut(s)	2529
BmeT110I	CYCGRG	1 cut(s)	2146
BmgBI	CACGTC	1 cut(s)	142
BmgT120I	GGNCC	4 cut(s)	467, 538, 1273, 1719
BmrFI	CCNGG	7 cut(s)	345, 459, 1277, 1501, 2565, 3158, 3259
BmrI	ACTGGG	3 cut(s)	810, 1235, 2228
BmsI	GCATC	4 cut(s)	76, 675, 1522, 2713
BmtI	GCTAGC	1 cut(s)	2925
BmuI	ACTGGG	3 cut(s)	810, 1235, 2228
BpiI	GAAGAC	1 cut(s)	2208
BplI	GAGNNNNNCTC	2 cut(s)	293, 325
BpmI	CTGGAG	1 cut(s)	1463
Bpu10I	CCTNAGC	1 cut(s)	2025
Bpu1102I	GCTNAGC	2 cut(s)	975, 3117
Bpu14I	TTCGAA	1 cut(s)	41
BpuEI	CTTGAG	3 cut(s)	521, 2057, 3093
BpuMI	CCSGG	2 cut(s)	345, 1277
BsaAI	YACGTR	1 cut(s)	2824
BsaBI	GATNNNNATC	2 cut(s)	1152, 1536
BsaHI	GRCGYC	1 cut(s)	1507
BsaI	GGTCTC	2 cut(s)	1325, 1377
BsaJI	CCNNGG	7 cut(s)	228, 534, 1854, 2313, 2564, 2790, 2938
BsaWI	WCCGGW	3 cut(s)	11, 967, 3222
BsaXI	ACNNNNNCTCC	8 cut(s)	79, 109, 1897, 1927, 2100, 2130, 2739, 2769
Bse118I	RCCGGY	1 cut(s)	55
Bse1I	ACTGG	8 cut(s)	82, 805, 1230, 1609, 2223, 2511, 2906, 3198
Bse21I	CCTNAGG	1 cut(s)	352
Bse3DI	GCAATG	5 cut(s)	444, 635, 853, 1051, 3052
Bse8I	GATNNNNATC	2 cut(s)	1152, 1536
BseAI	TCCGGA	1 cut(s)	967
BseBI	CCWGG	5 cut(s)	459, 1501, 2565, 3158, 3259
BseDI	CCNNGG	7 cut(s)	228, 534, 1854, 2313, 2564, 2790, 2938
BseGI	GGATG	6 cut(s)	381, 516, 666, 1537, 2299, 2728
BseJI	GATNNNNATC	2 cut(s)	1152, 1536
BseMI	GCAATG	5 cut(s)	444, 635, 853, 1051, 3052
BseMII	CTCAG	8 cut(s)	366, 879, 1434, 1559, 1673, 2517, 3005, 3108
BseNI	ACTGG	8 cut(s)	82, 805, 1230, 1609, 2223, 2511, 2906, 3198
BseRI	GAGGAG	1 cut(s)	224
BseSI	GKGCMC	1 cut(s)	3197
BseXI	GCAGC	8 cut(s)	871, 1204, 2290, 2314, 2582, 2713, 2764, 3014
BsgI	GTGCAG	3 cut(s)	590, 966, 1834
Bsh1236I	CGCG	4 cut(s)	223, 260, 320, 663
Bsh1285I	CGRYCG	2 cut(s)	32, 3274
BshFI	GGCC	3 cut(s)	101, 469, 681
BshNI	GGYRCC	1 cut(s)	2786
BsiEI	CGRYCG	2 cut(s)	32, 3274
BsiHKAI	GWGCWC	2 cut(s)	1072, 3197
BsiHKCI	CYCGRG	1 cut(s)	2146
BsiSI	CCGG	7 cut(s)	12, 56, 98, 344, 968, 1276, 3223
BslFI	GGGAC	3 cut(s)	524, 1298, 1595
BsmAI	GTCTC	6 cut(s)	1325, 1377, 1423, 2250, 2474, 3111
BsmFI	GGGAC	3 cut(s)	524, 1298, 1595
BsmI	GAATGC	1 cut(s)	1495
BsnI	GGCC	3 cut(s)	101, 469, 681
Bso31I	GGTCTC	2 cut(s)	1325, 1377
BsoBI	CYCGRG	1 cut(s)	2146
Bsp119I	TTCGAA	1 cut(s)	41
Bsp1286I	GDGCHC	3 cut(s)	241, 1072, 3197
Bsp13I	TCCGGA	1 cut(s)	967
Bsp1720I	GCTNAGC	2 cut(s)	975, 3117
Bsp19I	CCATGG	1 cut(s)	2938
BspACI	CCGC	8 cut(s)	32, 59, 248, 258, 320, 663, 1493, 2985
BspANI	GGCC	3 cut(s)	101, 469, 681
BspCNI	CTCAG	8 cut(s)	365, 878, 1433, 1558, 1672, 2518, 3006, 3109
BspEI	TCCGGA	1 cut(s)	967
BspFNI	CGCG	4 cut(s)	223, 260, 320, 663
BspHI	TCATGA	2 cut(s)	1050, 2607
BspMAI	CTGCAG	2 cut(s)	889, 2332
BspMI	ACCTGC	1 cut(s)	938
BspOI	GCTAGC	1 cut(s)	2925
BspPI	GGATC	8 cut(s)	3, 977, 1035, 1148, 2024, 2037, 2607, 2807
BspQI	GCTCTTC	2 cut(s)	701, 1971
BspT104I	TTCGAA	1 cut(s)	41
BspT107I	GGYRCC	1 cut(s)	2786
BspTI	CTTAAG	1 cut(s)	1058
BspTNI	GGTCTC	2 cut(s)	1325, 1377
BsrBI	CCGCTC	1 cut(s)	250
BsrDI	GCAATG	5 cut(s)	444, 635, 853, 1051, 3052
BsrFI	RCCGGY	1 cut(s)	55
BsrI	ACTGG	8 cut(s)	82, 805, 1230, 1609, 2223, 2511, 2906, 3198
BssAI	RCCGGY	1 cut(s)	55
BssECI	CCNNGG	7 cut(s)	228, 534, 1854, 2313, 2564, 2790, 2938
BssNAI	GTATAC	1 cut(s)	1958
BssNI	GRCGYC	1 cut(s)	1507
BssSI	CACGAG	3 cut(s)	1596, 2519, 2950
BssT1I	CCWWGG	5 cut(s)	534, 1854, 2313, 2790, 2938
Bst1107I	GTATAC	1 cut(s)	1958
Bst2BI	CACGAG	3 cut(s)	1596, 2519, 2950
Bst2UI	CCWGG	5 cut(s)	459, 1501, 2565, 3158, 3259
Bst6I	CTCTTC	9 cut(s)	129, 701, 1020, 1251, 1971, 2408, 3070, 3120, 3180
BstACI	GRCGYC	1 cut(s)	1507
BstAFI	CTTAAG	1 cut(s)	1058
BstAPI	GCANNNNNTGC	1 cut(s)	1945
BstBAI	YACGTR	1 cut(s)	2824
BstBI	TTCGAA	1 cut(s)	41
BstC8I	GCNNGC	6 cut(s)	360, 451, 655, 2711, 2882, 2923
BstDSI	CCRYGG	1 cut(s)	2938
BstEII	GGTNACC	1 cut(s)	385
BstENI	CCTNNNNNAGG	3 cut(s)	1367, 2039, 2317
BstF5I	GGATG	6 cut(s)	381, 516, 666, 1537, 2299, 2728
BstFNI	CGCG	4 cut(s)	223, 260, 320, 663
BstH2I	RGCGCY	1 cut(s)	2738
BstHHI	GCGC	3 cut(s)	1745, 2737, 3062
BstMAI	GTCTC	6 cut(s)	1325, 1377, 1423, 2250, 2474, 3111
BstMCI	CGRYCG	2 cut(s)	32, 3274
BstMWI	GCNNNNNNNGC	7 cut(s)	220, 663, 1945, 2732, 2786, 3024, 3066
BstNI	CCWGG	5 cut(s)	459, 1501, 2565, 3158, 3259
BstNSI	RCATGY	2 cut(s)	1904, 2713
BstPI	GGTNACC	1 cut(s)	385
BstSCI	CCNGG	7 cut(s)	343, 457, 1275, 1499, 2563, 3156, 3257
BstSFI	CTRYAG	6 cut(s)	885, 2328, 2730, 2828, 2844, 3252
BstSLI	GKGCMC	1 cut(s)	3197
BstUI	CGCG	4 cut(s)	223, 260, 320, 663
BstV1I	GCAGC	8 cut(s)	871, 1204, 2290, 2314, 2582, 2713, 2764, 3014
BstV2I	GAAGAC	1 cut(s)	2208
BstX2I	RGATCY	5 cut(s)	8, 868, 2029, 2599, 2812
BstXI	CCANNNNNNTGG	1 cut(s)	2368
BstYI	RGATCY	5 cut(s)	8, 868, 2029, 2599, 2812
BstZ17I	GTATAC	1 cut(s)	1958
Bsu36I	CCTNAGG	1 cut(s)	352
BsuRI	GGCC	3 cut(s)	101, 469, 681
BtgI	CCRYGG	1 cut(s)	2938
BtgZI	GCGATG	1 cut(s)	2321
BtrI	CACGTC	1 cut(s)	142
BtsCI	GGATG	6 cut(s)	381, 516, 666, 1537, 2299, 2728
BtsIMutI	CAGTG	8 cut(s)	75, 409, 1223, 1808, 2086, 2112, 2216, 3158
BveI	ACCTGC	1 cut(s)	938
Cac8I	GCNNGC	6 cut(s)	360, 451, 655, 2711, 2882, 2923
CciI	TCATGA	2 cut(s)	1050, 2607
CfoI	GCGC	3 cut(s)	1745, 2737, 3062
Cfr10I	RCCGGY	1 cut(s)	55
Cfr13I	GGNCC	4 cut(s)	467, 538, 1273, 1719
CseI	GACGC	1 cut(s)	1496
Csp6I	GTAC	9 cut(s)	166, 794, 862, 923, 1040, 1813, 1874, 2486, 2756
CviQI	GTAC	9 cut(s)	166, 794, 862, 923, 1040, 1813, 1874, 2486, 2756
DraI	TTTAAA	1 cut(s)	2016
DrdI	GACNNNNNNGTC	1 cut(s)	1060
DriI	GACNNNNNGTC	1 cut(s)	2529
DseDI	GACNNNNNNGTC	1 cut(s)	1060
EaeI	YGGCCR	1 cut(s)	99
Eam1104I	CTCTTC	9 cut(s)	129, 701, 1020, 1251, 1971, 2408, 3070, 3120, 3180
Eam1105I	GACNNNNNGTC	1 cut(s)	2529
EarI	CTCTTC	9 cut(s)	129, 701, 1020, 1251, 1971, 2408, 3070, 3120, 3180
Eco130I	CCWWGG	5 cut(s)	534, 1854, 2313, 2790, 2938
Eco147I	AGGCCT	1 cut(s)	681
Eco24I	GRGCYC	1 cut(s)	241
Eco31I	GGTCTC	2 cut(s)	1325, 1377
Eco32I	GATATC	1 cut(s)	1162
Eco47I	GGWCC	3 cut(s)	538, 1273, 1719
Eco57I	CTGAAG	3 cut(s)	641, 1001, 1887
Eco81I	CCTNAGG	1 cut(s)	352
Eco88I	CYCGRG	1 cut(s)	2146
Eco91I	GGTNACC	1 cut(s)	385
EcoNI	CCTNNNNNAGG	3 cut(s)	1367, 2039, 2317
EcoO109I	RGGNCCY	1 cut(s)	538
EcoO65I	GGTNACC	1 cut(s)	385
EcoRI	GAATTC	2 cut(s)	271, 1411
EcoRII	CCWGG	5 cut(s)	457, 1499, 2563, 3156, 3257
EcoRV	GATATC	1 cut(s)	1162
EcoT14I	CCWWGG	5 cut(s)	534, 1854, 2313, 2790, 2938
EcoT22I	ATGCAT	1 cut(s)	1537
EcoT38I	GRGCYC	1 cut(s)	241
ErhI	CCWWGG	5 cut(s)	534, 1854, 2313, 2790, 2938
FalI	AAGNNNNNCTT	4 cut(s)	1968, 2000, 3159, 3191
FaqI	GGGAC	3 cut(s)	524, 1298, 1595
FauI	CCCGC	2 cut(s)	52, 265
FauNDI	CATATG	1 cut(s)	721
FblI	GTMKAC	3 cut(s)	1957, 2059, 2094
Fnu4HI	GCNGC	9 cut(s)	664, 885, 1218, 2304, 2328, 2571, 2727, 2778, 3028
FokI	GGATG	6 cut(s)	388, 523, 653, 1544, 2306, 2735
FriOI	GRGCYC	1 cut(s)	241
Fsp4HI	GCNGC	9 cut(s)	664, 885, 1218, 2304, 2328, 2571, 2727, 2778, 3028
FspBI	CTAG	4 cut(s)	113, 1124, 2157, 2922
FspI	TGCGCA	1 cut(s)	3061
GlaI	GCGC	3 cut(s)	1744, 2736, 3061
GluI	GCNGC	9 cut(s)	664, 885, 1218, 2304, 2328, 2571, 2727, 2778, 3028
GsuI	CTGGAG	1 cut(s)	1463
HaeII	RGCGCY	1 cut(s)	2738
HaeIII	GGCC	3 cut(s)	101, 469, 681
HapII	CCGG	7 cut(s)	12, 56, 98, 344, 968, 1276, 3223
HgaI	GACGC	1 cut(s)	1496
HhaI	GCGC	3 cut(s)	1745, 2737, 3062
Hin1I	GRCGYC	1 cut(s)	1507
Hin6I	GCGC	3 cut(s)	1743, 2735, 3060
HinP1I	GCGC	3 cut(s)	1743, 2735, 3060
HpaII	CCGG	7 cut(s)	12, 56, 98, 344, 968, 1276, 3223
HphI	GGTGA	6 cut(s)	92, 905, 1022, 1870, 2647, 2705
Hpy99I	CGWCG	1 cut(s)	176
HpyCH4IV	ACGT	4 cut(s)	141, 556, 2823, 3102
HpyF10VI	GCNNNNNNNGC	7 cut(s)	220, 663, 1945, 2732, 2786, 3024, 3066
HpySE526I	ACGT	4 cut(s)	141, 556, 2823, 3102
Hsp92I	GRCGYC	1 cut(s)	1507
HspAI	GCGC	3 cut(s)	1743, 2735, 3060
Kpn2I	TCCGGA	1 cut(s)	967
LguI	GCTCTTC	2 cut(s)	701, 1971
LmnI	GCTCC	8 cut(s)	211, 334, 367, 916, 1075, 2108, 2929, 3279
Lsp1109I	GCAGC	8 cut(s)	871, 1204, 2290, 2314, 2582, 2713, 2764, 3014
LweI	GCATC	4 cut(s)	76, 675, 1522, 2713
MaeI	CTAG	4 cut(s)	113, 1124, 2157, 2922
MaeII	ACGT	4 cut(s)	141, 556, 2823, 3102
MbiI	CCGCTC	1 cut(s)	250
MfeI	CAATTG	4 cut(s)	296, 842, 1338, 1785
MflI	RGATCY	5 cut(s)	8, 868, 2029, 2599, 2812
MhlI	GDGCHC	3 cut(s)	241, 1072, 3197
MlyI	GAGTC	6 cut(s)	302, 334, 505, 1048, 1280, 2539
MmeI	TCCRAC	4 cut(s)	316, 754, 2725, 3257
Mph1103I	ATGCAT	1 cut(s)	1537
MroI	TCCGGA	1 cut(s)	967
MroXI	GAANNNNTTC	2 cut(s)	710, 1980
MseI	TTAA	9 cut(s)	552, 1059, 1394, 1935, 2015, 2406, 2511, 2535, 2679
MslI	CAYNNNNRTG	4 cut(s)	374, 1856, 2340, 3183
MspA1I	CMGCKG	2 cut(s)	2573, 2985
MspCI	CTTAAG	1 cut(s)	1058
MspI	CCGG	7 cut(s)	12, 56, 98, 344, 968, 1276, 3223
MspR9I	CCNGG	7 cut(s)	345, 459, 1277, 1501, 2565, 3158, 3259
MunI	CAATTG	4 cut(s)	296, 842, 1338, 1785
Mva1269I	GAATGC	1 cut(s)	1495
MvaI	CCWGG	5 cut(s)	459, 1501, 2565, 3158, 3259
MvnI	CGCG	4 cut(s)	223, 260, 320, 663
MwoI	GCNNNNNNNGC	7 cut(s)	220, 663, 1945, 2732, 2786, 3024, 3066
NciI	CCSGG	2 cut(s)	345, 1277
NcoI	CCATGG	1 cut(s)	2938
NdeI	CATATG	1 cut(s)	721
NheI	GCTAGC	1 cut(s)	2921
NmeAIII	GCCGAG	2 cut(s)	209, 3109
NmuCI	GTSAC	6 cut(s)	200, 1858, 1903, 2521, 2625, 2945
NsbI	TGCGCA	1 cut(s)	3061
NsiI	ATGCAT	1 cut(s)	1537
NspI	RCATGY	2 cut(s)	1904, 2713
NspV	TTCGAA	1 cut(s)	41
OliI	CACNNNNGTG	2 cut(s)	1856, 2340
PaeI	GCATGC	1 cut(s)	2713
PaeR7I	CTCGAG	1 cut(s)	2146
PagI	TCATGA	2 cut(s)	1050, 2607
PceI	AGGCCT	1 cut(s)	681
PciI	ACATGT	1 cut(s)	1900
PciSI	GCTCTTC	2 cut(s)	701, 1971
PctI	GAATGC	1 cut(s)	1495
PdmI	GAANNNNTTC	2 cut(s)	710, 1980
PfeI	GAWTC	9 cut(s)	83, 185, 218, 518, 1141, 1885, 2264, 2475, 2869
PflMI	CCANNNNNTGG	2 cut(s)	298, 2744
PfoI	TCCNGGA	1 cut(s)	457
PkrI	GCNGC	9 cut(s)	665, 886, 1219, 2305, 2329, 2572, 2728, 2779, 3029
PleI	GAGTC	6 cut(s)	302, 333, 504, 1048, 1280, 2538
PpsI	GAGTC	6 cut(s)	302, 333, 504, 1048, 1280, 2538
Ppu21I	YACGTR	1 cut(s)	2824
PpuMI	RGGWCCY	1 cut(s)	538
PscI	ACATGT	1 cut(s)	1900
PsiI	TTATAA	1 cut(s)	1176
Psp5II	RGGWCCY	1 cut(s)	538
Psp6I	CCWGG	5 cut(s)	457, 1499, 2563, 3156, 3257
PspEI	GGTNACC	1 cut(s)	385
PspGI	CCWGG	5 cut(s)	457, 1499, 2563, 3156, 3257
PspPI	GGNCC	4 cut(s)	467, 538, 1273, 1719
PspPPI	RGGWCCY	1 cut(s)	538
PspXI	VCTCGAGB	1 cut(s)	2146
PstI	CTGCAG	2 cut(s)	889, 2332
PsuI	RGATCY	5 cut(s)	8, 868, 2029, 2599, 2812
PvuII	CAGCTG	1 cut(s)	2573
RsaI	GTAC	9 cut(s)	167, 795, 863, 924, 1041, 1814, 1875, 2487, 2757
RsaNI	GTAC	9 cut(s)	166, 794, 862, 923, 1040, 1813, 1874, 2486, 2756
RseI	CAYNNNNRTG	4 cut(s)	374, 1856, 2340, 3183
SapI	GCTCTTC	2 cut(s)	701, 1971
SaqAI	TTAA	9 cut(s)	552, 1059, 1394, 1935, 2015, 2406, 2511, 2535, 2679
SatI	GCNGC	9 cut(s)	664, 885, 1218, 2304, 2328, 2571, 2727, 2778, 3028
Sau96I	GGNCC	4 cut(s)	467, 538, 1273, 1719
SchI	GAGTC	6 cut(s)	302, 334, 505, 1048, 1280, 2539
ScrFI	CCNGG	7 cut(s)	345, 459, 1277, 1501, 2565, 3158, 3259
SduI	GDGCHC	3 cut(s)	241, 1072, 3197
SfaNI	GCATC	4 cut(s)	76, 675, 1522, 2713
SfcI	CTRYAG	6 cut(s)	885, 2328, 2730, 2828, 2844, 3252
Sfr274I	CTCGAG	1 cut(s)	2146
SfuI	TTCGAA	1 cut(s)	41
SgrAI	CRCCGGYG	1 cut(s)	55
SinI	GGWCC	3 cut(s)	538, 1273, 1719
SlaI	CTCGAG	1 cut(s)	2146
SmiMI	CAYNNNNRTG	4 cut(s)	374, 1856, 2340, 3183
SmlI	CTYRAG	5 cut(s)	500, 1058, 2036, 2146, 3108
SmoI	CTYRAG	5 cut(s)	500, 1058, 2036, 2146, 3108
SphI	GCATGC	1 cut(s)	2713
SseBI	AGGCCT	1 cut(s)	681
SsiI	CCGC	8 cut(s)	32, 59, 248, 258, 320, 663, 1493, 2985
SspI	AATATT	2 cut(s)	733, 3216
SspMI	CTAG	4 cut(s)	113, 1124, 2157, 2922
StuI	AGGCCT	1 cut(s)	681
StyD4I	CCNGG	7 cut(s)	343, 457, 1275, 1499, 2563, 3156, 3257
StyI	CCWWGG	5 cut(s)	534, 1854, 2313, 2790, 2938
TaiI	ACGT	4 cut(s)	144, 559, 2826, 3105
TatI	WGTACW	2 cut(s)	861, 922
TauI	GCSGC	1 cut(s)	666
TfiI	GAWTC	9 cut(s)	83, 185, 218, 518, 1141, 1885, 2264, 2475, 2869
Tru1I	TTAA	9 cut(s)	552, 1059, 1394, 1935, 2015, 2406, 2511, 2535, 2679
Tru9I	TTAA	9 cut(s)	552, 1059, 1394, 1935, 2015, 2406, 2511, 2535, 2679
TscAI	CASTG	8 cut(s)	82, 409, 1230, 1815, 2086, 2119, 2223, 3158
TseFI	GTSAC	6 cut(s)	200, 1858, 1903, 2521, 2625, 2945
TseI	GCWGC	8 cut(s)	884, 1217, 2303, 2327, 2570, 2726, 2777, 3027
Tsp45I	GTSAC	6 cut(s)	200, 1858, 1903, 2521, 2625, 2945
TspGWI	ACGGA	4 cut(s)	188, 737, 908, 2276
TspRI	CASTG	8 cut(s)	82, 409, 1230, 1815, 2086, 2119, 2223, 3158
Van91I	CCANNNNNTGG	2 cut(s)	298, 2744
Vha464I	CTTAAG	1 cut(s)	1058
VneI	GTGCAC	1 cut(s)	3193
VpaK11BI	GGWCC	3 cut(s)	538, 1273, 1719
XagI	CCTNNNNNAGG	3 cut(s)	1367, 2039, 2317
XapI	RAATTY	9 cut(s)	122, 271, 545, 1187, 1238, 1411, 1968, 2086, 2207
XceI	RCATGY	2 cut(s)	1904, 2713
XhoI	CTCGAG	1 cut(s)	2146
XmiI	GTMKAC	3 cut(s)	1957, 2059, 2094
XmnI	GAANNNNTTC	2 cut(s)	710, 1980
XspI	CTAG	4 cut(s)	113, 1124, 2157, 2922
Zsp2I	ATGCAT	1 cut(s)	1537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.