Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0007117

Physical Location & Seq

Genomic Coordinates Reverse (-)

9937449 .. 9958774

Gene Structure 21326 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1053300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3261 bp

ATGAACAAAGGCATAAGCAGAAAACGGCCAACGCCGCCACAACAGGCGGCGAACCCATCCAAGCAGCAGGCCACGTCCCCAGAAGACGAATTCGTGGACGAGGACGAGGACGTCTTCCTCGACGAAACCCTTTTCGAGACTGAAGACGACCTTGTTCTCCGCGACATCGAGCAGCGCCAGTCCCTAGCTTCCCGCCTTACCAAGTGGGCCCGCCCGCCTCCCTCCCATGCCTACACTTCCGCTTCCCGAAGCGTCGTCTTTCAGCAATTGGAGATTGATTATGTAATTGGAGAGAGCCATAGAGAATTGCTGCCTAATTGGTCCGGGTCTGCTGCCATTATCCGAATTTTTGGGGTTACAAAGGAAGGACATAGTGTTTGCTGCCATGTTCATGGATTTGAGCCATATTTCTATATCAACTGTTTCCCCGTTATGGGCCCTGACGATATTTCCAGGTTTCATCAAATTCTTGAGGGGAGGATGGGAGAGGTCAACAGGAACAGCAAGGTACCAAAATTCATTCGCCGTATTGAAATGGTGCAGAAGAGGAGTATCATGTATTACCAGCAGCAGGATTCTGAACCCTTTCTCAAAATCGTTGTTGCTTTGCCAACAATGGTGGCCAGCTGCCGGGGCATTCTTGAAAAAGGCATACTAATTGATGGCCTTGGTATGAAGAAATTCATGACATATGATAGCAATGTTCTTTTTGCTCTTCGTTTCATGATTGATTGCAACATAGTTGGTGGAAATTGGATTGAGGTCCCTGCTGGAAGATATAAGACAACTAAGCCCTTGTCGTACTGCCAGTTAGAGTTCCATTGCCTATATTCGGACTTGATCAGCCATACTCCAGAAGGGGAATTTTCGAAGATGGCTCCATTTCGCATATTGAGCTTTGATATTGAGTGTGCTGGTCGTAAAGGTCATTTTCCTGAGCCTACCCATGATCCTGTTATCCAGGTGGCCAACTTAGTTACTATGCAGGGAGAGAATCAACCTTTTATTCGGAACATCATGACTCTTAAGTCGTGTGCTCCAATAGTTGGTGTTGATGTGATGTCTTTCGATACAGAAAGGGAAGTCTTGCTGGCTTGGAGGGATTTACTCCGCGAGGTGGATCCAGATGTCATAATTGGATATAACATCTGTAAATTTGATTTGCCATATCTCATTGAGAGAGCTGAGACGTTGGGAATTGCTGAATTTCCGATACTTGGCCGCATAAAAAACAGCAGGGTTCGGGTCAAAGACACTACTTTTTCTTCAAGGCAGCATGGAACCAGGGAAAGTAAAGAAGTAACAGTTGAAGGGAGGGTTCAATTTGATTTACTCCAGGCTATGCAACGAGATTACAAATTAAGTTCTTATTCATTGAATTCTGTCTCAGCACACTTTCTTTCTGAGCAGAAAGAGGATGTCCACCATTCAATTATATCTGATCTTCAGAACGGGAATGCGGAAACTAGGAGGCGACTTGCTGTGTATTGTCTGAAAGATGCTTACCTCCCACAAAGGCTTTTGGACAAACTGATGTATATTTACAATTATGTAGAGATGGCTAGAGTGACAGGTGTTCCAATGTCATTTCTTCTTTCCAGAGGACAGAGCATCAAGGTCCTTTCGCAGCTTCTTAGGAAGGCAAAGCAAAAGAACCTGCTTATTCCATATGTTAAAAAGGCTGGATCAGAGCAAGGAACGTATGAAGGTGCCACTGTTTTGGAAGCGAAGGCAGGATTCTATGAAAAGCCAATTGCCACTTTGGATTTTGCATCTTTATATCCATCAATCATGATGGCCTATAATCTATGCTATTGCACTTTGGTGACACCTGAAGATGTTCGCAAACTCAATATCCCTCCAGAATGTGTCAACAAAACTCCATCTGGAGAAACATTTGTTAAATCCAATTTGCAGAAGGGAATACTTCCTGAAATTCTTGAAGAATTATTAGCTGCTCGGAAGAGAGCAAAAGCGGATTTGAAGGAAGCAAAAGATCCACTTGAGAAGGCAGTGCTTGATGGTCGACAATTGGCCTTAAAGGTAAGTGCCAATTCCGTATATGGGTTTACTGGAGCTACTGTTGGCCAGTTACCATGTTTAGAGATATCATCAAGTGTCACCAGCTATGGTCGACAGATGATTGAGCACACAAAGAACCTTGTAAATGACAAATTTACCACACTTAAAGGCTATGAACACAATGCTGAGGTTATATACGGGGACACAGACTCAGTTATGGTGCAATTTGGGGTTCCCACAGTTGAAGCAGCAATGGACTTGGGGAGAGAAGCTGCTGAATACATAAGTGGAACTTTCATAAAACCTATCAAACTTGAATTTGAGAAGGTTTATCATCCATATCTTCTGATTAGCAAGAAGAGATATGCTGGTTTATTGTGGACAAATCCTGGCAAGTTTGACAAAATGGACACTAAAGGAATCGAAACCGTAAGAAGAGACAATTGCTTATTGGTCAAAAACCTGGTAAATGAATGCCTGCACAAAATACTGATTGATAGAGATATTCCTGGGGCAGTCCAGTATGTCAAGAATACCATCTCAGATCTTCTCATGAATCGCATGGATTTGTCTCTTTTGGTTATCACTAAGGGTCTTACAAAAACGGGGGATGATTATGGAGTAAAGGCAGCTCATGTTGAACTTGCTGAACGTATGCGCAAGCGAGATGCTGCCACTGCTCCAAATGTTGGGGATCGGGTGCCTTATGTCATTATTAAAGCTGCAAAAGGTGCCAAGGCTTACGAGAAGTCAGAGGATCCCATCTATGTGCTTGAAAATAACATTCCCATAGATCCTCAATACTATCTTGAAAATCAAATTAGCAAGCCACTCTTAAGACTTTTTGAGCCCATCTTGAAGAATGCTAGCAAGGAACTTCTCCATGGAAGCCACACAAGATCTATTTCAATCTCCACCCCTGCAAACAGTGGAATAATGAGATTTGCAAAGAAACAACTCACATGCGTTGGCTGCAAAGCTCTACTTAGCAAGTCGGACCATACTCTTTGCTCACACTGTAAGGGAAGAGAAGCTGAACTCTATTGCAAAACAGTGGCTCATGTGTCTGAGCTAGAGACGCTTTTTGGGCGGTTATGGACACAGTGCCAAGAGTGCCAAGGCTCTCTGCATCAAGATGTGCTCTGCACAAGTCGGGACTGCCCTATATTCTATAGGAGAAAGAAGGCACAGAAAGACATGGCTGAAGCTAAAGTGCAGTTAGACCGGTGGAGCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008296 GO:0008408 GO:0009058 GO:0009059 GO:0009987 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0030894 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0033554 GO:0034641 GO:0034645 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0043625 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0140097 GO:1901360 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko03430 ko03440 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map03430 map03440 map05166

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 zf-C4pol

Protein Analysis

1087

Amino Acids

122.89

Weight (kDa)

8.33

Isoelectric Point (pI)

41.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
POL3_N	PF24055	132 - 207	2.1e-06	DNA polymerase delta catalytic subunit-like, N-terminal domain
DNA_pol_B_exo1	PF03104	230 - 462	3e-69	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	526 - 959	2e-161	DNA polymerase family B
zf-C4pol	PF14260	996 - 1067	6.3e-19	C4-type zinc-finger of DNA polymerase delta

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0007117)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63960 AT5G63960
fragaria_vesca	FvH4_1g24660 FvH4_1g24660 FvH4_1g24660 FvH4_1g24660 FvH4_1g24660
malus_domestica	MD00G1053300.v1.1 MD05G1053800.v1.1 MD07G1029900.v1.1
prunus_persica	Prupe.4G273600_v2.0.a1 Prupe.4G273600_v2.0.a1 Prupe.4G273600_v2.0.a1
pyrus_communis	pycom01g00060 pycom07g02190
rosa_chinensis	RchiOBHm_Chr2g0122111
rosa_laevigata	RLG00000018650
rosa_multiflora	Rmu_co8081762.1_g000001
rosa_roxburghii	Rroxscaffold_2G00122010
rosa_rugosa	Rorug02G0236500
rosa_samantha	Rh2AG293000 Rh2BG303400 Rh2CG281900 Rh2DG317000
rosa_wichuraiana	Rw2G023500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	110, 1027
AatII	GACGTC	1 cut(s)	114
Acc16I	TGCGCA	1 cut(s)	2681
Acc36I	ACCTGC	1 cut(s)	1665
Acc65I	GGTACC	1 cut(s)	508
AccB1I	GGYRCC	4 cut(s)	508, 1709, 2722, 2753
AccB7I	CCANNNNNTGG	2 cut(s)	1046, 2711
AccI	GTMKAC	2 cut(s)	2026, 2134
AccII	CGCG	2 cut(s)	162, 1113
AclWI	GGATC	9 cut(s)	944, 1115, 1128, 1693, 1991, 2724, 2774, 2787, 2810
AcoI	YGGCCR	5 cut(s)	26, 621, 966, 1219, 2086
AcuI	CTGAAG	4 cut(s)	162, 1430, 1854, 3246
AcyI	GRCGYC	1 cut(s)	111
AfaI	GTAC	2 cut(s)	510, 803
AflII	CTTAAG	2 cut(s)	1025, 2857
AgeI	ACCGGT	1 cut(s)	3246
AjiI	CACGTC	1 cut(s)	75
AjnI	CCWGG	7 cut(s)	452, 960, 1283, 1335, 2410, 2484, 2530
AleI	CACNNNNGTG	1 cut(s)	1823
Alw21I	GWGCWC	3 cut(s)	1039, 2151, 3165
Alw26I	GTCTC	6 cut(s)	131, 1181, 1390, 2454, 2598, 3092
AlwI	GGATC	9 cut(s)	944, 1115, 1128, 1693, 1991, 2724, 2774, 2787, 2810
ApaI	GGGCCC	2 cut(s)	211, 440
ArsI	GACNNNNNNTTYG	2 cut(s)	360, 392
AsiGI	ACCGGT	1 cut(s)	3246
Asp700I	GAANNNNTTC	3 cut(s)	585, 680, 1839
Asp718I	GGTACC	1 cut(s)	508
AspLEI	GCGC	2 cut(s)	177, 2682
AspS9I	GGNCC	8 cut(s)	207, 208, 321, 436, 437, 763, 1618, 3019
AsuC2I	CCSGG	2 cut(s)	325, 632
AsuHPI	GGTGA	2 cut(s)	1837, 2113
AsuII	TTCGAA	1 cut(s)	869
AsuNHI	GCTAGC	1 cut(s)	2888
AvaII	GGWCC	4 cut(s)	321, 763, 1618, 3019
BaeGI	GKGCMC	2 cut(s)	211, 440
BalI	TGGCCA	3 cut(s)	623, 968, 2088
BamHI	GGATCC	2 cut(s)	1120, 2779
BanI	GGYRCC	4 cut(s)	508, 1709, 2722, 2753
BanII	GRGCYC	3 cut(s)	211, 440, 2874
BbsI	GAAGAC	3 cut(s)	90, 106, 150
Bbv12I	GWGCWC	3 cut(s)	1039, 2151, 3165
BbvCI	CCTCAGC	1 cut(s)	2208
BceAI	ACGGC	2 cut(s)	41, 510
BciT130I	CCWGG	7 cut(s)	454, 962, 1285, 1337, 2412, 2486, 2532
BclI	TGATCA	1 cut(s)	840
BcnI	CCSGG	2 cut(s)	325, 632
BcoDI	GTCTC	6 cut(s)	131, 1181, 1390, 2454, 2598, 3092
BfaI	CTAG	5 cut(s)	185, 1467, 1563, 2889, 3095
BfmI	CTRYAG	1 cut(s)	3193
BfoI	RGCGCY	1 cut(s)	178
BfrI	CTTAAG	2 cut(s)	1025, 2857
BfuAI	ACCTGC	1 cut(s)	1665
BglII	AGATCT	2 cut(s)	2566, 2921
Bme1390I	CCNGG	9 cut(s)	325, 454, 632, 962, 1285, 1337, 2412, 2486, 2532
Bme18I	GGWCC	4 cut(s)	321, 763, 1618, 3019
BmgBI	CACGTC	1 cut(s)	75
BmgT120I	GGNCC	8 cut(s)	207, 208, 321, 436, 437, 763, 1618, 3019
BmrFI	CCNGG	9 cut(s)	325, 454, 632, 962, 1285, 1337, 2412, 2486, 2532
BmsI	GCATC	5 cut(s)	1489, 1620, 1781, 2680, 3160
BmtI	GCTAGC	1 cut(s)	2892
BpiI	GAAGAC	3 cut(s)	90, 106, 150
BpmI	CTGGAG	5 cut(s)	837, 1319, 1845, 1908, 2094
Bpu10I	CCTNAGC	2 cut(s)	936, 2208
Bpu14I	TTCGAA	1 cut(s)	869
BpuEI	CTTGAG	2 cut(s)	491, 2024
BpuMI	CCSGG	2 cut(s)	325, 632
BsaHI	GRCGYC	1 cut(s)	111
BsaJI	CCNNGG	7 cut(s)	631, 667, 1284, 2531, 2757, 2905, 3139
BsaWI	WCCGGW	1 cut(s)	3246
BsaXI	ACNNNNNCTCC	2 cut(s)	2067, 2097
Bse118I	RCCGGY	1 cut(s)	3246
Bse1I	ACTGG	5 cut(s)	178, 808, 2077, 2089, 2542
Bse3DI	GCAATG	3 cut(s)	706, 820, 2280
BseBI	CCWGG	7 cut(s)	454, 962, 1285, 1337, 2412, 2486, 2532
BseDI	CCNNGG	7 cut(s)	631, 667, 1284, 2531, 2757, 2905, 3139
BseGI	GGATG	5 cut(s)	56, 486, 1423, 2356, 2638
BseMI	GCAATG	3 cut(s)	706, 820, 2280
BseMII	CTCAG	8 cut(s)	927, 1176, 1395, 1401, 2199, 2247, 2577, 3081
BseNI	ACTGG	5 cut(s)	178, 808, 2077, 2089, 2542
BseRI	GAGGAG	1 cut(s)	562
BseSI	GKGCMC	2 cut(s)	211, 440
BsgI	GTGCAG	4 cut(s)	560, 2486, 3151, 3257
Bsh1236I	CGCG	2 cut(s)	162, 1113
BshNI	GGYRCC	4 cut(s)	508, 1709, 2722, 2753
BshTI	ACCGGT	1 cut(s)	3246
BsiHKAI	GWGCWC	3 cut(s)	1039, 2151, 3165
BsiSI	CCGG	3 cut(s)	324, 631, 3247
BslFI	GGGAC	5 cut(s)	61, 166, 749, 2237, 3191
BsmAI	GTCTC	6 cut(s)	131, 1181, 1390, 2454, 2598, 3092
BsmBI	CGTCTC	2 cut(s)	1181, 3092
BsmFI	GGGAC	5 cut(s)	61, 166, 749, 2237, 3191
BsmI	GAATGC	4 cut(s)	636, 1462, 2501, 2890
Bsp119I	TTCGAA	1 cut(s)	869
Bsp120I	GGGCCC	2 cut(s)	207, 436
Bsp1286I	GDGCHC	6 cut(s)	211, 440, 1039, 2151, 2874, 3165
Bsp19I	CCATGG	1 cut(s)	2905
BspCNI	CTCAG	8 cut(s)	928, 1177, 1396, 1400, 2200, 2246, 2576, 3082
BspFNI	CGCG	2 cut(s)	162, 1113
BspHI	TCATGA	5 cut(s)	684, 723, 1017, 1791, 2574
BspMI	ACCTGC	1 cut(s)	1665
BspOI	GCTAGC	1 cut(s)	2892
BspPI	GGATC	9 cut(s)	944, 1115, 1128, 1693, 1991, 2724, 2774, 2787, 2810
BspQI	GCTCTTC	1 cut(s)	720
BspT104I	TTCGAA	1 cut(s)	869
BspT107I	GGYRCC	4 cut(s)	508, 1709, 2722, 2753
BspTI	CTTAAG	2 cut(s)	1025, 2857
BsrDI	GCAATG	3 cut(s)	706, 820, 2280
BsrFI	RCCGGY	1 cut(s)	3246
BsrI	ACTGG	5 cut(s)	178, 808, 2077, 2089, 2542
BssAI	RCCGGY	1 cut(s)	3246
BssECI	CCNNGG	7 cut(s)	631, 667, 1284, 2531, 2757, 2905, 3139
BssNI	GRCGYC	1 cut(s)	111
BssT1I	CCWWGG	4 cut(s)	667, 2757, 2905, 3139
Bst2UI	CCWGG	7 cut(s)	454, 962, 1285, 1337, 2412, 2486, 2532
Bst6I	CTCTTC	6 cut(s)	539, 720, 1958, 2375, 2452, 3043
BstACI	GRCGYC	1 cut(s)	111
BstAFI	CTTAAG	2 cut(s)	1025, 2857
BstAPI	GCANNNNNTGC	1 cut(s)	2753
BstBI	TTCGAA	1 cut(s)	869
BstC8I	GCNNGC	9 cut(s)	69, 211, 215, 625, 1092, 2501, 2684, 2849, 2890
BstDSI	CCRYGG	1 cut(s)	2905
BstF5I	GGATG	5 cut(s)	56, 486, 1423, 2356, 2638
BstFNI	CGCG	2 cut(s)	162, 1113
BstH2I	RGCGCY	1 cut(s)	178
BstHHI	GCGC	2 cut(s)	177, 2682
BstMAI	GTCTC	6 cut(s)	131, 1181, 1390, 2454, 2598, 3092
BstMWI	GCNNNNNNNGC	9 cut(s)	34, 633, 894, 2699, 2753, 2994, 3100, 3109, 3135
BstNI	CCWGG	7 cut(s)	454, 962, 1285, 1337, 2412, 2486, 2532
BstNSI	RCATGY	1 cut(s)	2988
BstSCI	CCNGG	9 cut(s)	323, 452, 630, 960, 1283, 1335, 2410, 2484, 2530
BstSFI	CTRYAG	1 cut(s)	3193
BstSLI	GKGCMC	2 cut(s)	211, 440
BstUI	CGCG	2 cut(s)	162, 1113
BstV2I	GAAGAC	3 cut(s)	90, 106, 150
BstX2I	RGATCY	6 cut(s)	1120, 1996, 2566, 2779, 2815, 2921
BstXI	CCANNNNNNTGG	2 cut(s)	392, 1720
BstYI	RGATCY	6 cut(s)	1120, 1996, 2566, 2779, 2815, 2921
BtgI	CCRYGG	1 cut(s)	2905
BtrI	CACGTC	1 cut(s)	75
BtsCI	GGATG	5 cut(s)	56, 486, 1423, 2356, 2638
BtsI	GCAGTG	2 cut(s)	2019, 2697
BtsIMutI	CAGTG	7 cut(s)	1713, 2019, 2697, 2956, 3037, 3081, 3131
BveI	ACCTGC	1 cut(s)	1665
Cac8I	GCNNGC	9 cut(s)	69, 211, 215, 625, 1092, 2501, 2684, 2849, 2890
CciI	TCATGA	5 cut(s)	684, 723, 1017, 1791, 2574
CfoI	GCGC	2 cut(s)	177, 2682
Cfr10I	RCCGGY	1 cut(s)	3246
Cfr13I	GGNCC	8 cut(s)	207, 208, 321, 436, 437, 763, 1618, 3019
CseI	GACGC	2 cut(s)	241, 3109
CsiI	ACCWGGT	1 cut(s)	2484
Csp6I	GTAC	2 cut(s)	509, 802
CspAI	ACCGGT	1 cut(s)	3246
CspCI	CAANNNNNGTGG	2 cut(s)	600, 635
CviQI	GTAC	2 cut(s)	509, 802
DrdI	GACNNNNNNGTC	2 cut(s)	110, 1027
DseDI	GACNNNNNNGTC	2 cut(s)	110, 1027
EaeI	YGGCCR	5 cut(s)	26, 621, 966, 1219, 2086
Eam1104I	CTCTTC	6 cut(s)	539, 720, 1958, 2375, 2452, 3043
EarI	CTCTTC	6 cut(s)	539, 720, 1958, 2375, 2452, 3043
Eco130I	CCWWGG	4 cut(s)	667, 2757, 2905, 3139
Eco24I	GRGCYC	3 cut(s)	211, 440, 2874
Eco32I	GATATC	1 cut(s)	2109
Eco47I	GGWCC	4 cut(s)	321, 763, 1618, 3019
Eco57I	CTGAAG	4 cut(s)	162, 1430, 1854, 3246
EcoO109I	RGGNCCY	3 cut(s)	437, 763, 1618
EcoRI	GAATTC	2 cut(s)	89, 1378
EcoRII	CCWGG	7 cut(s)	452, 960, 1283, 1335, 2410, 2484, 2530
EcoRV	GATATC	1 cut(s)	2109
EcoT14I	CCWWGG	4 cut(s)	667, 2757, 2905, 3139
EcoT38I	GRGCYC	3 cut(s)	211, 440, 2874
ErhI	CCWWGG	4 cut(s)	667, 2757, 2905, 3139
Esp3I	CGTCTC	2 cut(s)	1181, 3092
FaqI	GGGAC	5 cut(s)	61, 166, 749, 2237, 3191
FauI	CCCGC	3 cut(s)	200, 218, 222
FauNDI	CATATG	2 cut(s)	691, 1669
FbaI	TGATCA	1 cut(s)	840
FblI	GTMKAC	2 cut(s)	2026, 2134
FokI	GGATG	5 cut(s)	43, 493, 1430, 2343, 2645
FriOI	GRGCYC	3 cut(s)	211, 440, 2874
FspBI	CTAG	5 cut(s)	185, 1467, 1563, 2889, 3095
FspI	TGCGCA	1 cut(s)	2681
GlaI	GCGC	2 cut(s)	176, 2681
GsuI	CTGGAG	5 cut(s)	837, 1319, 1845, 1908, 2094
HaeII	RGCGCY	1 cut(s)	178
HapII	CCGG	3 cut(s)	324, 631, 3247
HgaI	GACGC	2 cut(s)	241, 3109
HhaI	GCGC	2 cut(s)	177, 2682
Hin1I	GRCGYC	1 cut(s)	111
Hin6I	GCGC	2 cut(s)	175, 2680
HinP1I	GCGC	2 cut(s)	175, 2680
HincII	GTYRAC	4 cut(s)	493, 1873, 2027, 2135
HindII	GTYRAC	4 cut(s)	493, 1873, 2027, 2135
HinfI	GANTC	7 cut(s)	575, 994, 1021, 1737, 2231, 2442, 2578
HpaII	CCGG	3 cut(s)	324, 631, 3247
HphI	GGTGA	2 cut(s)	1837, 2113
Hpy166II	GTNNAC	8 cut(s)	97, 493, 1423, 1873, 2027, 2070, 2135, 2403
Hpy8I	GTNNAC	8 cut(s)	97, 493, 1423, 1873, 2027, 2070, 2135, 2403
Hpy99I	CGWCG	2 cut(s)	125, 257
HpyCH4IV	ACGT	5 cut(s)	74, 111, 1190, 1700, 2674
HpyF10VI	GCNNNNNNNGC	9 cut(s)	34, 633, 894, 2699, 2753, 2994, 3100, 3109, 3135
HpySE526I	ACGT	5 cut(s)	74, 111, 1190, 1700, 2674
Hsp92I	GRCGYC	1 cut(s)	111
HspAI	GCGC	2 cut(s)	175, 2680
KpnI	GGTACC	1 cut(s)	512
Ksp22I	TGATCA	1 cut(s)	840
LguI	GCTCTTC	1 cut(s)	720
LmnI	GCTCC	5 cut(s)	883, 1042, 2075, 2707, 3252
LweI	GCATC	5 cut(s)	1489, 1620, 1781, 2680, 3160
MabI	ACCWGGT	1 cut(s)	2484
MaeI	CTAG	5 cut(s)	185, 1467, 1563, 2889, 3095
MaeII	ACGT	5 cut(s)	74, 111, 1190, 1700, 2674
MaeIII	GTNAC	7 cut(s)	355, 976, 1300, 1567, 1825, 2091, 2119
MfeI	CAATTG	4 cut(s)	266, 1752, 2030, 2464
MflI	RGATCY	6 cut(s)	1120, 1996, 2566, 2779, 2815, 2921
MhlI	GDGCHC	6 cut(s)	211, 440, 1039, 2151, 2874, 3165
MlsI	TGGCCA	3 cut(s)	623, 968, 2088
MluNI	TGGCCA	3 cut(s)	623, 968, 2088
MlyI	GAGTC	2 cut(s)	1015, 2225
MmeI	TCCRAC	1 cut(s)	2997
Mox20I	TGGCCA	3 cut(s)	623, 968, 2088
MroXI	GAANNNNTTC	3 cut(s)	585, 680, 1839
MscI	TGGCCA	3 cut(s)	623, 968, 2088
MseI	TTAA	8 cut(s)	1026, 1361, 1674, 1902, 2039, 2187, 2739, 2858
MslI	CAYNNNNRTG	4 cut(s)	390, 1823, 2789, 3156
Msp20I	TGGCCA	3 cut(s)	623, 968, 2088
MspA1I	CMGCKG	1 cut(s)	627
MspCI	CTTAAG	2 cut(s)	1025, 2857
MspI	CCGG	3 cut(s)	324, 631, 3247
MspR9I	CCNGG	9 cut(s)	325, 454, 632, 962, 1285, 1337, 2412, 2486, 2532
MunI	CAATTG	4 cut(s)	266, 1752, 2030, 2464
Mva1269I	GAATGC	4 cut(s)	636, 1462, 2501, 2890
MvaI	CCWGG	7 cut(s)	454, 962, 1285, 1337, 2412, 2486, 2532
MvnI	CGCG	2 cut(s)	162, 1113
MwoI	GCNNNNNNNGC	9 cut(s)	34, 633, 894, 2699, 2753, 2994, 3100, 3109, 3135
NciI	CCSGG	2 cut(s)	325, 632
NcoI	CCATGG	1 cut(s)	2905
NdeI	CATATG	2 cut(s)	691, 1669
NheI	GCTAGC	1 cut(s)	2888
NmuCI	GTSAC	3 cut(s)	1567, 1825, 2119
NsbI	TGCGCA	1 cut(s)	2681
NspI	RCATGY	1 cut(s)	2988
NspV	TTCGAA	1 cut(s)	869
OliI	CACNNNNGTG	1 cut(s)	1823
PagI	TCATGA	5 cut(s)	684, 723, 1017, 1791, 2574
PciSI	GCTCTTC	1 cut(s)	720
PcsI	WCGNNNNNNNCGW	1 cut(s)	117
PctI	GAATGC	4 cut(s)	636, 1462, 2501, 2890
PdmI	GAANNNNTTC	3 cut(s)	585, 680, 1839
PfeI	GAWTC	5 cut(s)	575, 994, 1737, 2442, 2578
PflMI	CCANNNNNTGG	2 cut(s)	1046, 2711
PinAI	ACCGGT	1 cut(s)	3246
PleI	GAGTC	2 cut(s)	1015, 2225
PpsI	GAGTC	2 cut(s)	1015, 2225
PpuMI	RGGWCCY	2 cut(s)	763, 1618
Psp5II	RGGWCCY	2 cut(s)	763, 1618
Psp6I	CCWGG	7 cut(s)	452, 960, 1283, 1335, 2410, 2484, 2530
PspGI	CCWGG	7 cut(s)	452, 960, 1283, 1335, 2410, 2484, 2530
PspOMI	GGGCCC	2 cut(s)	207, 436
PspPI	GGNCC	8 cut(s)	207, 208, 321, 436, 437, 763, 1618, 3019
PspPPI	RGGWCCY	2 cut(s)	763, 1618
PsuI	RGATCY	6 cut(s)	1120, 1996, 2566, 2779, 2815, 2921
PvuII	CAGCTG	1 cut(s)	627
RsaI	GTAC	2 cut(s)	510, 803
RsaNI	GTAC	2 cut(s)	509, 802
RseI	CAYNNNNRTG	4 cut(s)	390, 1823, 2789, 3156
SalI	GTCGAC	2 cut(s)	2025, 2133
SapI	GCTCTTC	1 cut(s)	720
SaqAI	TTAA	8 cut(s)	1026, 1361, 1674, 1902, 2039, 2187, 2739, 2858
Sau96I	GGNCC	8 cut(s)	207, 208, 321, 436, 437, 763, 1618, 3019
SchI	GAGTC	2 cut(s)	1015, 2225
ScrFI	CCNGG	9 cut(s)	325, 454, 632, 962, 1285, 1337, 2412, 2486, 2532
SduI	GDGCHC	6 cut(s)	211, 440, 1039, 2151, 2874, 3165
SexAI	ACCWGGT	1 cut(s)	2484
SfaNI	GCATC	5 cut(s)	1489, 1620, 1781, 2680, 3160
SfcI	CTRYAG	1 cut(s)	3193
SfuI	TTCGAA	1 cut(s)	869
SinI	GGWCC	4 cut(s)	321, 763, 1618, 3019
SmiMI	CAYNNNNRTG	4 cut(s)	390, 1823, 2789, 3156
SmlI	CTYRAG	4 cut(s)	470, 1025, 2003, 2857
SmoI	CTYRAG	4 cut(s)	470, 1025, 2003, 2857
SspMI	CTAG	5 cut(s)	185, 1467, 1563, 2889, 3095
StyD4I	CCNGG	9 cut(s)	323, 452, 630, 960, 1283, 1335, 2410, 2484, 2530
StyI	CCWWGG	4 cut(s)	667, 2757, 2905, 3139
TaiI	ACGT	5 cut(s)	77, 114, 1193, 1703, 2677
TaqI	TCGA	8 cut(s)	120, 135, 168, 869, 1068, 2026, 2134, 2445
TauI	GCSGC	3 cut(s)	37, 50, 1224
TfiI	GAWTC	5 cut(s)	575, 994, 1737, 2442, 2578
Tru1I	TTAA	8 cut(s)	1026, 1361, 1674, 1902, 2039, 2187, 2739, 2858
Tru9I	TTAA	8 cut(s)	1026, 1361, 1674, 1902, 2039, 2187, 2739, 2858
TscAI	CASTG	7 cut(s)	1720, 2019, 2704, 2956, 3044, 3081, 3131
TseFI	GTSAC	3 cut(s)	1567, 1825, 2119
Tsp45I	GTSAC	3 cut(s)	1567, 1825, 2119
TspGWI	ACGGA	1 cut(s)	2047
TspRI	CASTG	7 cut(s)	1720, 2019, 2704, 2956, 3044, 3081, 3131
Van91I	CCANNNNNTGG	2 cut(s)	1046, 2711
Vha464I	CTTAAG	2 cut(s)	1025, 2857
VpaK11BI	GGWCC	4 cut(s)	321, 763, 1618, 3019
XceI	RCATGY	1 cut(s)	2988
XmiI	GTMKAC	2 cut(s)	2026, 2134
XmnI	GAANNNNTTC	3 cut(s)	585, 680, 1839
XspI	CTAG	5 cut(s)	185, 1467, 1563, 2889, 3095
ZraI	GACGTC	1 cut(s)	112

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.