Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0007117

Physical Location & Seq

Genomic Coordinates Forward (+)

39341822 .. 39357966

Gene Structure 16145 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG317000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3084 bp

ATGAGCAACAATGGCAAAACCCGAAAACGGCCGGCACCTCCTCAGCAGCCGTCGCCGGTGAACCCACCACCGCCCAAGCAGCGCGCCGCGTCTCAAGAAGAAGAGGAATTTCTCGACGAGGACGTCTTCCTCGACGAAACCCTACTCGAGACCGAAGACGACCTCATTCTCCGCGACAGTGAGCAGCGCCAATCCCTCGCCTCCCGCCTCACCAGGTGGGCCCGCCCACCTCTCTCCGATGCCTACAAGTCCGCCTCCAAAAGCATCCTGTTTCAGCAATTGGAGATTGACTATGTGATTGGAGAGAGCAATAAAGAATTGTTGCCTAATTGGTCCGGTCCTGCTGCCATTATCAGAATATTTGGGGTTACAAAGGAAGGACATAGTGTTTGCTGCCACGTTCATGGATTCGAGCCATATTTCTATATCAACTGCCCTCCAGGGATGGGACATGATGATATTCCTCGGTTTCGTCAGACTCTCGAGGCGAAGATGGGGGAGGCAAACAGGAACAATAAGGCATCATTGTTCATTAGACGTGTTGAAATGGTGTACAAGAGGAATATAATGTATTACCAGCAGCAGGATTCCAATCCCTTTCTCAAAATTGTTGTTTCTTTGCCAACAATGGTGGCCCGCTGCCGGGGTATTCTTGATAAAGGCATACAAATTGATGGTGTTGGTGAGAAGAGCTTCATGACATATGAAAGCAATGTTCTGTTTGCTCTTCGATTCATGATTGATTGCAACATAGTTGGTGGAAATTGGATTGAGGTACCTGCCGGAAAATATAAGACAGCAAAGAGTACATCGTACTGCCAATTAGAGTTCAATTGCCTATATTCAGACTTGATCAGCCATGCTCCTGAAGGTGAATTTTCGAAGATGGCTCCGTTTCGCATTTTAAGCTTTGACATCGAGTGTGCTGGACGCAAAGGTCATTTTCCTGATGCCAAGCATGATCCTGTCATCCAGGTGGCCAACCTAGTTACTTTGCAGGGAGAGAATCAGCCATTTATTCGTAATGTTATGACTCTTAAGTCATGCTCCCCTATAGCTGGTGCTGATGTGATTTCCTTTGATACAGAAAGAGAAGTCTTGCTGGCTTGGAGGGACTTAATCCGTGAGGCAGACCCAGATGTCATGATTGGATATAACATCTGTAAATTTGATTTGCCATATCTCATTGAGAGAGCTGAGACTTTGGGAATACCTGAATTTCCAATACTTGGGCGCATCAGAAACAGCAGGGTTCGGGTCAAAGACACTACTTTTTCTTCAAGGCAGATGGGAACCAGGGAAAGTAAAGAAGTAACAATTGAAGGGAGGGTTCAATTTGATATACTCCAGGTTATGCAACGGGATTATAAATTAAGCTCATATTCATTGAATTCTGTCTCAGCACACTTTCTTTCTGAGCAGAAAGAGGACGTCCACCATTCCATAATATCTGATCTTCAGAATGGCAATGCGGAAACTAGGAGGCGACTTGCTGAGTATTGTCTGAAGGATGCTTACCTCCCGCAGAGGCTTTTGGACAAACTTATGTTTATTTACAATTATGTAGAGATGGCTCGAGTCACTGGTGTTCCCATCTCATTTCTACTTTCCAGAGGGCAGAGCATCAAGGTGCTTTCTCAACTTCTTAGGAAGGCTAAACAAAAGAACCTTCTCCTTCCAAATGTCAAACAGGCTGGATCTGAACAAGGAACATATGAAGGTGCCACTGTTTTGGAAGCAAAGGCAGGATTTTATGAAAAGCCAATTGCAACTTTGGATTTTGCATCCCTGTACCCCTCAATCATGATGGCTTATAATCTATGCTATTGCACCCTGGTCCGATCTGAAGATGTACGCAAACTCAACCTTCCTCCAGAATGTGTGAACAAAACTCCATCTGGAGAAACATTTGTTAAACCGAATTTGCAAAAGGGAATTCTTCCTGAAATTCTTGAAGAACTAATAGCTGCTCGGAAGAGAGCAAAAGCGGATTTGAAGGAAGCAAAGGATCCGCTTGAGAAGGCAGTGCTAGATGGTCGACAATTGGCCTTAAAGATAAGTGCAAATTCCGTATATGGGTTTACCGGAGCTACAATTGGTCAATTACCATGTTTGGAGATATCATCAAGTGTCACGAGCTATGGTCGACAGATGATTGAGCACACAAAAAACCTTGTAAAAGAAAAATTTACTACCCTTGGAGGCTATGAACATAATGCCGAGGTAATATATGGGGACACAGATTCAGTTATGGTGCAATTCGGTGTAAGTACTGTTGAAGCAGCAATGAAATTGGGAAGAGAAGCTGCTGATGCTATCAGTGCCACGTTCACAAAGCCTATCAAACTCGAGTTTGAGAAAGTTTATTATCCATATCTTCTGATTAGCAAGAAGAGATATGCTGGTTTGTTTTGGACAAATCCAGAGAAGTTCGACAAAATGGATACTAAAGGCATTGAAACAGTACGAAGAGACAATTGCTTATTGGTAAAAAACCTGGTAAATGAGTGCTTGCACAAAATACTGATTGACAGAGATATTCCTGGGGCAGTCCAGTTTGTCAAGAACACTATATCTGATCTTCTTATGAACCGCATGGATTTGTCACTCTTGGTTATTACTAAGGGTCTGTCAAAAACTGGGGAGGATTATGGAGTAAAGGCAGCTCATGTTGAACTTGCTGAGCGTATGCGCAAGCGAGATGCTGCCACTGCTCCAACGGTTGGGGATCGAGTACCATATGTCATTATCAAAGCTGCAAAAGGTGCCAAGGCTTATGAGAGGTCAGAGGACCCCATCTATGTGCTCGAGAATAACATTCCTATAGACCCTCAATATTATCTTGAAAACCAAATCAGCAAGCCACTCTTAAGAATTTTTGAGCCTATTTTGAAGAATGCCAGCTCGGAACTTCTCCATGGAAGTCATACAAGATCTATTTCAATGCCTACTCCCTCCAACAGTGGCATAATGAGATTTGCAAAGAAACAACTCACTTGCCTTGGTTGTAAAACTCCAATTAGGTACCATTTGCAGATTTTTCGTATTCCTAAAAATGATTGCTTTCATCTGCAGGCCTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008296 GO:0008408 GO:0009058 GO:0009059 GO:0009987 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0030894 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0033554 GO:0034641 GO:0034645 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0043625 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0140097 GO:1901360 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko03430 ko03440 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map03430 map03440 map05166

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 zf-C4pol

Protein Analysis

1027

Amino Acids

116.01

Weight (kDa)

8.54

Isoelectric Point (pI)

45.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
POL3_N	PF24055	136 - 211	1.5e-06	DNA polymerase delta catalytic subunit-like, N-terminal domain
DNA_pol_B_exo1	PF03104	234 - 466	9e-71	DNA polymerase family B, exonuclease domain
DNA_pol_B_2	PF03175	472 - 622	5.1e-06	DNA polymerase type B, organellar and viral
DNA_pol_B	PF00136	530 - 963	2.2e-163	DNA polymerase family B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007117)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63960 AT5G63960
fragaria_vesca	FvH4_1g24660 FvH4_1g24660 FvH4_1g24660 FvH4_1g24660 FvH4_1g24660
malus_domestica	MD00G1053300.v1.1 MD05G1053800.v1.1 MD07G1029900.v1.1
prunus_persica	Prupe.4G273600_v2.0.a1 Prupe.4G273600_v2.0.a1 Prupe.4G273600_v2.0.a1
pyrus_communis	pycom01g00060 pycom07g02190
rosa_chinensis	RchiOBHm_Chr2g0122111
rosa_laevigata	RLG00000018650
rosa_multiflora	Rmu_co8081762.1_g000001
rosa_roxburghii	Rroxscaffold_2G00122010
rosa_rugosa	Rorug02G0236500
rosa_samantha	Rh2AG293000 Rh2BG303400 Rh2CG281900 Rh2DG317000
rosa_wichuraiana	Rw2G023500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1368, 1815
AasI	GACNNNNNNGTC	4 cut(s)	122, 936, 1039, 1139
AatII	GACGTC	2 cut(s)	126, 1434
Acc16I	TGCGCA	1 cut(s)	2693
Acc36I	ACCTGC	1 cut(s)	787
Acc65I	GGTACC	2 cut(s)	775, 3024
AccB1I	GGYRCC	5 cut(s)	34, 775, 1721, 2765, 3024
AccB7I	CCANNNNNTGG	2 cut(s)	1229, 2723
AccI	GTMKAC	2 cut(s)	2038, 2146
AccII	CGCG	3 cut(s)	84, 89, 174
AclWI	GGATC	5 cut(s)	956, 1705, 2003, 2016, 2736
AcoI	YGGCCR	2 cut(s)	29, 978
AcuI	CTGAAG	4 cut(s)	888, 1442, 1526, 1866
AcyI	GRCGYC	2 cut(s)	123, 1431
AdeI	CACNNNGTG	1 cut(s)	216
AfiI	CCNNNNNNNGG	9 cut(s)	27, 446, 583, 642, 643, 1058, 1229, 2076, 2723
AflII	CTTAAG	2 cut(s)	1037, 2869
AflIII	ACRYGT	1 cut(s)	538
AjiI	CACGTC	1 cut(s)	539
AjnI	CCWGG	8 cut(s)	212, 439, 972, 1295, 1347, 1833, 2496, 2542
Alw21I	GWGCWC	2 cut(s)	2163, 2808
Alw26I	GTCTC	5 cut(s)	96, 143, 1193, 1402, 2466
AlwI	GGATC	5 cut(s)	956, 1705, 2003, 2016, 2736
Ama87I	CYCGRG	5 cut(s)	146, 482, 1575, 2348, 2807
AoxI	GGCC	6 cut(s)	29, 219, 633, 978, 2046, 3075
ApaI	GGGCCC	1 cut(s)	223
ArsI	GACNNNNNNTTYG	2 cut(s)	372, 404
Asp700I	GAANNNNTTC	1 cut(s)	692
Asp718I	GGTACC	2 cut(s)	775, 3024
AspLEI	GCGC	5 cut(s)	84, 86, 189, 1236, 2694
AspS9I	GGNCC	7 cut(s)	219, 220, 333, 338, 634, 1837, 2791
AsuC2I	CCSGG	1 cut(s)	644
AsuHPI	GGTGA	4 cut(s)	70, 202, 695, 884
AsuII	TTCGAA	1 cut(s)	881
AvaI	CYCGRG	5 cut(s)	146, 482, 1575, 2348, 2807
AvaII	GGWCC	4 cut(s)	333, 338, 1837, 2791
BaeGI	GKGCMC	1 cut(s)	223
BalI	TGGCCA	1 cut(s)	980
BamHI	GGATCC	1 cut(s)	2008
BanI	GGYRCC	5 cut(s)	34, 775, 1721, 2765, 3024
BanII	GRGCYC	1 cut(s)	223
BarI	GAAGNNNNNNTAC	2 cut(s)	1499, 1531
BauI	CACGAG	1 cut(s)	2134
BbsI	GAAGAC	2 cut(s)	118, 162
Bbv12I	GWGCWC	2 cut(s)	2163, 2808
BbvCI	CCTCAGC	1 cut(s)	42
BceAI	ACGGC	2 cut(s)	34, 44
BcgI	CGANNNNNNTGC	2 cut(s)	3023, 3057
BciT130I	CCWGG	8 cut(s)	214, 441, 974, 1297, 1349, 1835, 2498, 2544
BciVI	GTATCC	1 cut(s)	2437
BclI	TGATCA	1 cut(s)	852
BcnI	CCSGG	1 cut(s)	644
BcoDI	GTCTC	5 cut(s)	96, 143, 1193, 1402, 2466
BfaI	CTAG	3 cut(s)	986, 1479, 2030
BfmI	CTRYAG	3 cut(s)	1053, 2823, 3071
BfoI	RGCGCY	1 cut(s)	190
BfrI	CTTAAG	2 cut(s)	1037, 2869
BfuAI	ACCTGC	1 cut(s)	787
BfuI	GTATCC	1 cut(s)	2437
BglII	AGATCT	1 cut(s)	2933
BlpI	GCTNAGC	1 cut(s)	2682
BmcAI	AGTACT	1 cut(s)	2272
Bme1390I	CCNGG	9 cut(s)	214, 441, 644, 974, 1297, 1349, 1835, 2498, 2544
Bme18I	GGWCC	4 cut(s)	333, 338, 1837, 2791
BmeT110I	CYCGRG	5 cut(s)	146, 482, 1575, 2348, 2807
BmgBI	CACGTC	1 cut(s)	539
BmgT120I	GGNCC	7 cut(s)	219, 220, 333, 338, 634, 1837, 2791
BmrFI	CCNGG	9 cut(s)	214, 441, 644, 974, 1297, 1349, 1835, 2498, 2544
BmrI	ACTGGG	1 cut(s)	2649
BmuI	ACTGGG	1 cut(s)	2649
BpiI	GAAGAC	2 cut(s)	118, 162
BpmI	CTGGAG	4 cut(s)	423, 1331, 1857, 1920
Bpu10I	CCTNAGC	1 cut(s)	42
Bpu1102I	GCTNAGC	1 cut(s)	2682
Bpu14I	TTCGAA	1 cut(s)	881
BpuEI	CTTGAG	2 cut(s)	78, 2036
BpuMI	CCSGG	1 cut(s)	644
BsaBI	GATNNNNATC	1 cut(s)	591
BsaHI	GRCGYC	2 cut(s)	123, 1431
BsaI	GGTCTC	1 cut(s)	143
BsaWI	WCCGGW	2 cut(s)	335, 2084
Bsc4I	CCNNNNNNNGG	9 cut(s)	27, 446, 583, 642, 643, 1058, 1229, 2076, 2723
Bse118I	RCCGGY	2 cut(s)	31, 55
Bse1I	ACTGG	3 cut(s)	1588, 2554, 2644
Bse3DI	GCAATG	3 cut(s)	718, 1474, 2292
Bse8I	GATNNNNATC	1 cut(s)	591
BseBI	CCWGG	8 cut(s)	214, 441, 974, 1297, 1349, 1835, 2498, 2544
BseGI	GGATG	5 cut(s)	264, 450, 969, 1516, 1784
BseJI	GATNNNNATC	1 cut(s)	591
BseLI	CCNNNNNNNGG	9 cut(s)	27, 446, 583, 642, 643, 1058, 1229, 2076, 2723
BseMI	GCAATG	3 cut(s)	718, 1474, 2292
BseMII	CTCAG	6 cut(s)	56, 1188, 1407, 1413, 1485, 2673
BseNI	ACTGG	3 cut(s)	1588, 2554, 2644
BsePI	GCGCGC	1 cut(s)	82
BseRI	GAGGAG	1 cut(s)	30
BseSI	GKGCMC	1 cut(s)	223
BseX3I	CGGCCG	1 cut(s)	29
Bsh1236I	CGCG	3 cut(s)	84, 89, 174
Bsh1285I	CGRYCG	1 cut(s)	32
BshFI	GGCC	6 cut(s)	31, 221, 635, 980, 2048, 3077
BshNI	GGYRCC	5 cut(s)	34, 775, 1721, 2765, 3024
BsiEI	CGRYCG	1 cut(s)	32
BsiHKAI	GWGCWC	2 cut(s)	2163, 2808
BsiHKCI	CYCGRG	5 cut(s)	146, 482, 1575, 2348, 2807
BsiSI	CCGG	6 cut(s)	32, 56, 336, 643, 783, 2085
BslFI	GGGAC	3 cut(s)	462, 1127, 2249
BslI	CCNNNNNNNGG	9 cut(s)	27, 446, 583, 642, 643, 1058, 1229, 2076, 2723
BsmAI	GTCTC	5 cut(s)	96, 143, 1193, 1402, 2466
BsmBI	CGTCTC	1 cut(s)	96
BsmFI	GGGAC	3 cut(s)	462, 1127, 2249
BsmI	GAATGC	1 cut(s)	2902
BsnI	GGCC	6 cut(s)	31, 221, 635, 980, 2048, 3077
Bso31I	GGTCTC	1 cut(s)	143
BsoBI	CYCGRG	5 cut(s)	146, 482, 1575, 2348, 2807
Bsp119I	TTCGAA	1 cut(s)	881
Bsp120I	GGGCCC	1 cut(s)	219
Bsp1286I	GDGCHC	3 cut(s)	223, 2163, 2808
Bsp1407I	TGTACA	1 cut(s)	552
Bsp143I	GATC	9 cut(s)	852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
Bsp1720I	GCTNAGC	1 cut(s)	2682
Bsp19I	CCATGG	1 cut(s)	2917
BspANI	GGCC	6 cut(s)	31, 221, 635, 980, 2048, 3077
BspCNI	CTCAG	6 cut(s)	55, 1189, 1408, 1412, 1486, 2674
BspFNI	CGCG	3 cut(s)	84, 89, 174
BspHI	TCATGA	4 cut(s)	696, 735, 1143, 1803
BspMAI	CTGCAG	1 cut(s)	3075
BspMI	ACCTGC	1 cut(s)	787
BspPI	GGATC	5 cut(s)	956, 1705, 2003, 2016, 2736
BspQI	GCTCTTC	2 cut(s)	683, 732
BspT104I	TTCGAA	1 cut(s)	881
BspT107I	GGYRCC	5 cut(s)	34, 775, 1721, 2765, 3024
BspTI	CTTAAG	2 cut(s)	1037, 2869
BspTNI	GGTCTC	1 cut(s)	143
BsrDI	GCAATG	3 cut(s)	718, 1474, 2292
BsrFI	RCCGGY	2 cut(s)	31, 55
BsrGI	TGTACA	1 cut(s)	552
BsrI	ACTGG	3 cut(s)	1588, 2554, 2644
BssAI	RCCGGY	2 cut(s)	31, 55
BssHII	GCGCGC	1 cut(s)	82
BssMI	GATC	9 cut(s)	852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
BssNI	GRCGYC	2 cut(s)	123, 1431
BssSI	CACGAG	1 cut(s)	2134
BssT1I	CCWWGG	4 cut(s)	2197, 2769, 2917, 3001
Bst2BI	CACGAG	1 cut(s)	2134
Bst2UI	CCWGG	8 cut(s)	214, 441, 974, 1297, 1349, 1835, 2498, 2544
Bst4CI	ACNGT	6 cut(s)	179, 1729, 2275, 2464, 2722, 2963
Bst6I	CTCTTC	7 cut(s)	96, 683, 732, 1970, 2293, 2387, 2464
BstACI	GRCGYC	2 cut(s)	123, 1431
BstAFI	CTTAAG	2 cut(s)	1037, 2869
BstAPI	GCANNNNNTGC	1 cut(s)	2765
BstAUI	TGTACA	1 cut(s)	552
BstBI	TTCGAA	1 cut(s)	881
BstDEI	CTNAG	8 cut(s)	42, 1197, 1399, 1416, 1494, 1646, 2622, 2682
BstDSI	CCRYGG	1 cut(s)	2917
BstF5I	GGATG	5 cut(s)	264, 450, 969, 1516, 1784
BstFNI	CGCG	3 cut(s)	84, 89, 174
BstH2I	RGCGCY	1 cut(s)	190
BstHHI	GCGC	5 cut(s)	84, 86, 189, 1236, 2694
BstKTI	GATC	9 cut(s)	855, 964, 1456, 1700, 1844, 2011, 2581, 2731, 2936
BstMAI	GTCTC	5 cut(s)	96, 143, 1193, 1402, 2466
BstMBI	GATC	9 cut(s)	852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
BstMCI	CGRYCG	1 cut(s)	32
BstMWI	GCNNNNNNNGC	8 cut(s)	12, 52, 79, 906, 2312, 2321, 2711, 2765
BstNI	CCWGG	8 cut(s)	214, 441, 974, 1297, 1349, 1835, 2498, 2544
BstSCI	CCNGG	9 cut(s)	212, 439, 642, 972, 1295, 1347, 1833, 2496, 2542
BstSFI	CTRYAG	3 cut(s)	1053, 2823, 3071
BstSLI	GKGCMC	1 cut(s)	223
BstUI	CGCG	3 cut(s)	84, 89, 174
BstV2I	GAAGAC	2 cut(s)	118, 162
BstX2I	RGATCY	3 cut(s)	1697, 2008, 2933
BstXI	CCANNNNNNTGG	2 cut(s)	404, 1732
BstYI	RGATCY	3 cut(s)	1697, 2008, 2933
BstZI	CGGCCG	1 cut(s)	29
BsuI	GTATCC	1 cut(s)	2437
BsuRI	GGCC	6 cut(s)	31, 221, 635, 980, 2048, 3077
BtgI	CCRYGG	1 cut(s)	2917
BtrI	CACGTC	1 cut(s)	539
BtsCI	GGATG	5 cut(s)	264, 450, 969, 1516, 1784
BtsI	GCAGTG	2 cut(s)	2031, 2709
BtsIMutI	CAGTG	7 cut(s)	184, 1581, 1725, 2031, 2326, 2709, 2968
BveI	ACCTGC	1 cut(s)	787
CciI	TCATGA	4 cut(s)	696, 735, 1143, 1803
CfoI	GCGC	5 cut(s)	84, 86, 189, 1236, 2694
Cfr10I	RCCGGY	2 cut(s)	31, 55
Cfr13I	GGNCC	7 cut(s)	219, 220, 333, 338, 634, 1837, 2791
CseI	GACGC	2 cut(s)	78, 939
CsiI	ACCWGGT	2 cut(s)	212, 2496
CspCI	CAANNNNNGTGG	2 cut(s)	612, 647
DdeI	CTNAG	8 cut(s)	42, 1197, 1399, 1416, 1494, 1646, 2622, 2682
DpnI	GATC	9 cut(s)	854, 963, 1455, 1699, 1843, 2010, 2580, 2730, 2935
DpnII	GATC	9 cut(s)	852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
DraIII	CACNNNGTG	1 cut(s)	216
DrdI	GACNNNNNNGTC	4 cut(s)	122, 936, 1039, 1139
DseDI	GACNNNNNNGTC	4 cut(s)	122, 936, 1039, 1139
EaeI	YGGCCR	2 cut(s)	29, 978
EagI	CGGCCG	1 cut(s)	29
Eam1104I	CTCTTC	7 cut(s)	96, 683, 732, 1970, 2293, 2387, 2464
EarI	CTCTTC	7 cut(s)	96, 683, 732, 1970, 2293, 2387, 2464
EciI	GGCGGA	1 cut(s)	241
EclXI	CGGCCG	1 cut(s)	29
Eco130I	CCWWGG	4 cut(s)	2197, 2769, 2917, 3001
Eco147I	AGGCCT	1 cut(s)	3077
Eco24I	GRGCYC	1 cut(s)	223
Eco31I	GGTCTC	1 cut(s)	143
Eco32I	GATATC	1 cut(s)	2121
Eco47I	GGWCC	4 cut(s)	333, 338, 1837, 2791
Eco52I	CGGCCG	1 cut(s)	29
Eco57I	CTGAAG	4 cut(s)	888, 1442, 1526, 1866
Eco88I	CYCGRG	5 cut(s)	146, 482, 1575, 2348, 2807
EcoO109I	RGGNCCY	1 cut(s)	2791
EcoRI	GAATTC	2 cut(s)	1390, 1935
EcoRII	CCWGG	8 cut(s)	212, 439, 972, 1295, 1347, 1833, 2496, 2542
EcoRV	GATATC	1 cut(s)	2121
EcoT14I	CCWWGG	4 cut(s)	2197, 2769, 2917, 3001
EcoT38I	GRGCYC	1 cut(s)	223
ErhI	CCWWGG	4 cut(s)	2197, 2769, 2917, 3001
Esp3I	CGTCTC	1 cut(s)	96
FalI	AAGNNNNNCTT	2 cut(s)	2852, 2884
FaqI	GGGAC	3 cut(s)	462, 1127, 2249
FauI	CCCGC	4 cut(s)	212, 230, 644, 1530
FauNDI	CATATG	3 cut(s)	703, 1714, 2740
FbaI	TGATCA	1 cut(s)	852
FblI	GTMKAC	2 cut(s)	2038, 2146
FokI	GGATG	5 cut(s)	251, 457, 956, 1523, 1771
FriOI	GRGCYC	1 cut(s)	223
FspBI	CTAG	3 cut(s)	986, 1479, 2030
FspI	TGCGCA	1 cut(s)	2693
GlaI	GCGC	5 cut(s)	83, 85, 188, 1235, 2693
GsuI	CTGGAG	4 cut(s)	423, 1331, 1857, 1920
HaeII	RGCGCY	1 cut(s)	190
HaeIII	GGCC	6 cut(s)	31, 221, 635, 980, 2048, 3077
HapII	CCGG	6 cut(s)	32, 56, 336, 643, 783, 2085
HgaI	GACGC	2 cut(s)	78, 939
HhaI	GCGC	5 cut(s)	84, 86, 189, 1236, 2694
Hin1I	GRCGYC	2 cut(s)	123, 1431
Hin6I	GCGC	5 cut(s)	82, 84, 187, 1234, 2692
HinP1I	GCGC	5 cut(s)	82, 84, 187, 1234, 2692
HincII	GTYRAC	2 cut(s)	2039, 2147
HindII	GTYRAC	2 cut(s)	2039, 2147
HindIII	AAGCTT	1 cut(s)	907
HinfI	GANTC	8 cut(s)	408, 478, 587, 732, 1006, 1033, 1578, 2243
HpaII	CCGG	6 cut(s)	32, 56, 336, 643, 783, 2085
HphI	GGTGA	4 cut(s)	70, 202, 695, 884
Hpy166II	GTNNAC	8 cut(s)	61, 553, 1435, 1885, 2039, 2082, 2147, 2331
Hpy8I	GTNNAC	8 cut(s)	61, 553, 1435, 1885, 2039, 2082, 2147, 2331
Hpy99I	CGWCG	3 cut(s)	55, 119, 137
HpyCH4III	ACNGT	6 cut(s)	179, 1729, 2275, 2464, 2722, 2963
HpyCH4IV	ACGT	5 cut(s)	123, 399, 538, 1431, 2327
HpyF10VI	GCNNNNNNNGC	8 cut(s)	12, 52, 79, 906, 2312, 2321, 2711, 2765
HpyF3I	CTNAG	8 cut(s)	42, 1197, 1399, 1416, 1494, 1646, 2622, 2682
HpySE526I	ACGT	5 cut(s)	123, 399, 538, 1431, 2327
Hsp92I	GRCGYC	2 cut(s)	123, 1431
HspAI	GCGC	5 cut(s)	82, 84, 187, 1234, 2692
KpnI	GGTACC	2 cut(s)	779, 3028
KroI	GCCGGC	1 cut(s)	31
KroNI	GCCGGC	1 cut(s)	33
Ksp22I	TGATCA	1 cut(s)	852
Kzo9I	GATC	9 cut(s)	852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
LguI	GCTCTTC	2 cut(s)	683, 732
LmnI	GCTCC	5 cut(s)	868, 895, 1052, 2087, 2719
MabI	ACCWGGT	2 cut(s)	212, 2496
MaeI	CTAG	3 cut(s)	986, 1479, 2030
MaeII	ACGT	5 cut(s)	123, 399, 538, 1431, 2327
MaeIII	GTNAC	6 cut(s)	367, 988, 1312, 1579, 2131, 2604
MalI	GATC	9 cut(s)	854, 963, 1455, 1699, 1843, 2010, 2580, 2730, 2935
MboI	GATC	9 cut(s)	852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
MfeI	CAATTG	7 cut(s)	278, 832, 1317, 1764, 2042, 2094, 2476
MflI	RGATCY	3 cut(s)	1697, 2008, 2933
MhlI	GDGCHC	3 cut(s)	223, 2163, 2808
MlsI	TGGCCA	1 cut(s)	980
MluNI	TGGCCA	1 cut(s)	980
MlyI	GAGTC	3 cut(s)	472, 1027, 1587
MmeI	TCCRAC	2 cut(s)	2741, 2982
Mox20I	TGGCCA	1 cut(s)	980
MroNI	GCCGGC	1 cut(s)	31
MroXI	GAANNNNTTC	1 cut(s)	692
MscI	TGGCCA	1 cut(s)	980
MseI	TTAA	7 cut(s)	905, 1038, 1118, 1373, 1914, 2051, 2870
MslI	CAYNNNNRTG	4 cut(s)	402, 974, 1628, 2801
Msp20I	TGGCCA	1 cut(s)	980
MspA1I	CMGCKG	1 cut(s)	639
MspCI	CTTAAG	2 cut(s)	1037, 2869
MspI	CCGG	6 cut(s)	32, 56, 336, 643, 783, 2085
MspR9I	CCNGG	9 cut(s)	214, 441, 644, 974, 1297, 1349, 1835, 2498, 2544
MunI	CAATTG	7 cut(s)	278, 832, 1317, 1764, 2042, 2094, 2476
Mva1269I	GAATGC	1 cut(s)	2902
MvaI	CCWGG	8 cut(s)	214, 441, 974, 1297, 1349, 1835, 2498, 2544
MvnI	CGCG	3 cut(s)	84, 89, 174
MwoI	GCNNNNNNNGC	8 cut(s)	12, 52, 79, 906, 2312, 2321, 2711, 2765
NaeI	GCCGGC	1 cut(s)	33
NciI	CCSGG	1 cut(s)	644
NcoI	CCATGG	1 cut(s)	2917
NdeI	CATATG	3 cut(s)	703, 1714, 2740
NdeII	GATC	9 cut(s)	852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
NgoMIV	GCCGGC	1 cut(s)	31
NmeAIII	GCCGAG	1 cut(s)	2245
NmuCI	GTSAC	3 cut(s)	1579, 2131, 2604
NsbI	TGCGCA	1 cut(s)	2693
NspV	TTCGAA	1 cut(s)	881
PaeR7I	CTCGAG	5 cut(s)	146, 482, 1575, 2348, 2807
PagI	TCATGA	4 cut(s)	696, 735, 1143, 1803
PauI	GCGCGC	1 cut(s)	82
PceI	AGGCCT	1 cut(s)	3077
PciSI	GCTCTTC	2 cut(s)	683, 732
PcsI	WCGNNNNNNNCGW	2 cut(s)	120, 129
PctI	GAATGC	1 cut(s)	2902
PdiI	GCCGGC	1 cut(s)	33
PdmI	GAANNNNTTC	1 cut(s)	692
PfeI	GAWTC	5 cut(s)	408, 587, 732, 1006, 2243
PflMI	CCANNNNNTGG	2 cut(s)	1229, 2723
PleI	GAGTC	3 cut(s)	472, 1027, 1586
PpsI	GAGTC	3 cut(s)	472, 1027, 1586
PpuMI	RGGWCCY	1 cut(s)	2791
PsiI	TTATAA	2 cut(s)	1368, 1815
Psp5II	RGGWCCY	1 cut(s)	2791
Psp6I	CCWGG	8 cut(s)	212, 439, 972, 1295, 1347, 1833, 2496, 2542
PspGI	CCWGG	8 cut(s)	212, 439, 972, 1295, 1347, 1833, 2496, 2542
PspOMI	GGGCCC	1 cut(s)	219
PspPI	GGNCC	7 cut(s)	219, 220, 333, 338, 634, 1837, 2791
PspPPI	RGGWCCY	1 cut(s)	2791
PspXI	VCTCGAGB	2 cut(s)	1575, 2348
PstI	CTGCAG	1 cut(s)	3075
PsuI	RGATCY	3 cut(s)	1697, 2008, 2933
PteI	GCGCGC	1 cut(s)	82
RseI	CAYNNNNRTG	4 cut(s)	402, 974, 1628, 2801
SalI	GTCGAC	2 cut(s)	2037, 2145
SapI	GCTCTTC	2 cut(s)	683, 732
SaqAI	TTAA	7 cut(s)	905, 1038, 1118, 1373, 1914, 2051, 2870
Sau3AI	GATC	9 cut(s)	852, 961, 1453, 1697, 1841, 2008, 2578, 2728, 2933
Sau96I	GGNCC	7 cut(s)	219, 220, 333, 338, 634, 1837, 2791
ScaI	AGTACT	1 cut(s)	2272
SchI	GAGTC	3 cut(s)	472, 1027, 1587
ScrFI	CCNGG	9 cut(s)	214, 441, 644, 974, 1297, 1349, 1835, 2498, 2544
SduI	GDGCHC	3 cut(s)	223, 2163, 2808
SexAI	ACCWGGT	2 cut(s)	212, 2496
SfcI	CTRYAG	3 cut(s)	1053, 2823, 3071
Sfr274I	CTCGAG	5 cut(s)	146, 482, 1575, 2348, 2807
SfuI	TTCGAA	1 cut(s)	881
SgrAI	CRCCGGYG	1 cut(s)	55
SinI	GGWCC	4 cut(s)	333, 338, 1837, 2791
SlaI	CTCGAG	5 cut(s)	146, 482, 1575, 2348, 2807
SmiMI	CAYNNNNRTG	4 cut(s)	402, 974, 1628, 2801
SmlI	CTYRAG	9 cut(s)	93, 146, 482, 1037, 1575, 2015, 2348, 2807, 2869
SmoI	CTYRAG	9 cut(s)	93, 146, 482, 1037, 1575, 2015, 2348, 2807, 2869
SseBI	AGGCCT	1 cut(s)	3077
SspI	AATATT	2 cut(s)	360, 2837
SspMI	CTAG	3 cut(s)	986, 1479, 2030
StuI	AGGCCT	1 cut(s)	3077
StyD4I	CCNGG	9 cut(s)	212, 439, 642, 972, 1295, 1347, 1833, 2496, 2542
StyI	CCWWGG	4 cut(s)	2197, 2769, 2917, 3001
TaaI	ACNGT	6 cut(s)	179, 1729, 2275, 2464, 2722, 2963
TaiI	ACGT	5 cut(s)	126, 402, 541, 1434, 2330
TaqII	GACCGA	1 cut(s)	167
TatI	WGTACW	3 cut(s)	552, 806, 2270
TauI	GCSGC	1 cut(s)	89
TfiI	GAWTC	5 cut(s)	408, 587, 732, 1006, 2243
Tru1I	TTAA	7 cut(s)	905, 1038, 1118, 1373, 1914, 2051, 2870
Tru9I	TTAA	7 cut(s)	905, 1038, 1118, 1373, 1914, 2051, 2870
TscAI	CASTG	7 cut(s)	184, 1588, 1732, 2031, 2326, 2716, 2968
TseFI	GTSAC	3 cut(s)	1579, 2131, 2604
Tsp45I	GTSAC	3 cut(s)	1579, 2131, 2604
TspGWI	ACGGA	3 cut(s)	882, 1112, 2059
TspRI	CASTG	7 cut(s)	184, 1588, 1732, 2031, 2326, 2716, 2968
Van91I	CCANNNNNTGG	2 cut(s)	1229, 2723
Vha464I	CTTAAG	2 cut(s)	1037, 2869
VpaK11BI	GGWCC	4 cut(s)	333, 338, 1837, 2791
XhoI	CTCGAG	5 cut(s)	146, 482, 1575, 2348, 2807
XmiI	GTMKAC	2 cut(s)	2038, 2146
XmnI	GAANNNNTTC	1 cut(s)	692
XspI	CTAG	3 cut(s)	986, 1479, 2030
ZraI	GACGTC	2 cut(s)	124, 1432
ZrmI	AGTACT	1 cut(s)	2272

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.