Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007117

Physical Location & Seq

Genomic Coordinates Forward (+)

2489316 .. 2505402

Gene Structure 16087 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1029900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2925 bp

ATGAACAAAGGCATAAGCAGAAAACGTCCAGCGCCGCCACAACCGGCGGCGAACCCAGCGGAGCAGCAGGCCACGTCCCCAGAAGAAGAAGAAGAATTCGTAGACGAGGACGTCTTCCTCGACGAAACCCTTTTCGAGACTGAAGACAACCTTGTTCTCCGCGACATCGAGCAGCGCCAGTCCCTCGCTTCCCGCCTCACCAATTGGGCCCGCCCGCCTCTCTCCCATGCCTACACTTCCGCCTCCCGAAGCATCGCTTTTCAGCAATTGGAGATTGATTATGTAATTCGAGAGATCCATAGAGAATTGCTGCCTAATTGGTCCGGGTCCGCTGCCATTATCCGAATTTTTCGGGTTACAAAGGAAGGACATAGTGTTTGCTGCCATGTTCATGGATTTGAGCCATATTTCTATATCAACTGTTTCCCCGGAATGGGCCCTGACGATATTTCCAGGTTTCATCAAATTCTTGAGGTCAACAGGAACAGCAAGGTTCCAAAATTCATTCGCCGTATTGAAATGGTGCAGAAGAGGAGTATCATGTATTACCAGCAGCAGGATTCTGAACCCTTTCTCAAAATTGTTGTTGCTTTGCCAACAATGGTGGCCAGCTGCCGGGGCATTCTTGAAAAGGGCATACAAATTGATGGCCTTGGTATGAAGAAATTCATGACATATGACAGCAATGTTCTTTTTGCTCTTCGTTTCATGATTGATTGCAACATAGTTGGTGGAAATTGGATTGAGGTCCCTGCTGGTAAATATAAGACAACTAAGCCTTTGTCGTACTGCCAGTTAGAGTTCCATTGCCTATATTTAGACTTGATCAGCCATACTCCAGAAGGGGAATTTTCGAAGATGGCTCCATTTCGCATATTGAGCGTTGATATTGAGTGTGCTGGCCGTAAAGGTCATTTTCCTGAGCCTACCCATGATCCTGTTATCCAGGTGGCCAACCTAGTTACCATGCAGGGAGAGAACCAACCATTTATACGGAACGTCATGACTCTTAAGTCATGTGCTCCAATAGTTGGTGTTGATGTGATGCCTTTTGATACAGAAAGGGAAGTCTTGCTGGCTTGGAGGTATGGAACCAGGGAAAGTAAAGAAGTAACAGTTGAAGGGAGGGTTCAATTTGATTTACTCCAGGCTATGCAACGAGATTACAAATTAAGTTCTTATTCATTGAATTCTGTCTCAGCGCACTTTCTCTCTGAGCAGAAAGAGGATGTCCACCATTCAATTATATCTGATCTGCAGAATGGGAATGCGGAAACTAGGAGGCGATTTGCTGTATATTGTCTGAAAGATGCTTACCTCCCACAAAGGCTTTTGGACAAACTGATGTATATTTACAATTATGTAGAGATGGCTAGAGTGACAGGTGTTCCAATGTCATTTCTTCTTTCCAGAGGACAGAGCATCAAGGTACTTTTGCAGCTTCTTAGGAAGGCAAAGCAAAAGAACCTGCTTATTCCATATGTCAAAAAGGCTGGATCTGAGCAAGGAACGTATGAAGGTGCCACTGTTTTGGAAGCAAAGGCAGGATTCTATGAAAAGCCAATTGCCACTTTGGATTTTGCATCTTTATATCCATCAATCATGATGGCCTATGCTATTGCACTTTGTGAGAAACTTTGGCCAAAATGGTATCCCTTTTTCTATGTGAAGGTGAAACCTGAAGTTGTCCACAAACTCAATATCCCTCCAGAATGTGTCAACAAAACTCCATCTGGAGAAACATTTGTTAAATCCAATTTGCAGAAGGGAACACATCCTGAAATTCTTGAAGAATTATTAGCTGCTCGGAAGAGAGCAAAAGCGGATTTGAAGAAGGCAGTGCTTGATGGGCGACAATTGACCTTAAAGATAAGTGCCAATTCTGTATATGGGTTTACTGGAGCCACTGTGCCAATTACCATGTTTAGAGATATCATCAAGTGTCGACAGATGATTGAGCACACAAAGAACATTGTGAAAGACAAATTTACCACCCTTAAAGGCTATGAACACAATGCTGAGGTTATATATGGGGACACAGACTCAGTAATGGTGCAATTCGGGGTTCCCACTGTTGAAGCAGCAATGAACTTGGGGAGAGAAGCTGCTGAATACATCAGTGGAACTTTCATAACACCTATCAAACTCGAATTTGAGAAGGTTTATTATCCATATCTTCTGATAAGCAAGAAGAGATATGCTGGTTTATTGTGGACAAATCCTGGCAAGTTCGACAAAATGGACACTAAAGGAATCGAAACCGTACGAAGAGACAATTGCTTATTGGTCAAAAACCTGGTAAATGAATGCCTGCACAAAATACTGATTGACAGAGATATTCCTGGGGCAATCCAGTATGTCAAGAATACCATCTCAGATCTTCTCATGAATCGCATGGATTTGTCGCTTTTGGTTATCACTAAGGCAGCTCATGTTGAACTTGCTGAACGTATGCGCAAGCGAGATGCTGCTACTGCTCCAAATGTTGGGGATCGGGTACCTTATGTCATTATTAAAGCTGCAAAAGGTGCCAAGCCCATCTTGAAGAATGCTAGCAAGGAACTTCTCCATGGAAGTAACACAAGATCTATTTCAATCTCCACCCCAGCAAACAGTGGAATAATGAGATTTGCAAAGAAACAACTCACATGCGTTGGCTGCAAAGCTCTACTTAGCAAGGCGGACCGTACTCTTTGCTCACACTGCAAGGGAAGAGAAGCTGAACTCTATTGCAAAACAGTGGCTCATGTCTCTGAGTTAGAGATGCTTTTTGGGCGGTTATGGACGCAGTGCCAAGAGTGCCAAGGCTCTCTGCATCAAGATGTGCTCTGCACAAGTCGGAACTGCCCTATATTCTATCGGAGAAGGAAGGCACAGAAAGACATGGCTGAAGCTAAAGTGCAGTTAGACCGGTGGAGCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004529 GO:0004536 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006287 GO:0006289 GO:0006297 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008296 GO:0008408 GO:0009058 GO:0009059 GO:0009987 GO:0016787 GO:0016788 GO:0016796 GO:0016895 GO:0030894 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0033554 GO:0034641 GO:0034645 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0043625 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045004 GO:0045005 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0140097 GO:1901360 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko03430 ko03440 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map03430 map03440 map05166

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 zf-C4pol

Protein Analysis

975

Amino Acids

110.78

Weight (kDa)

8.97

Isoelectric Point (pI)

43.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_B_exo1	PF03104	225 - 361	1.1e-32	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	463 - 856	3.5e-107	DNA polymerase family B
zf-C4pol	PF14260	884 - 955	5.6e-18	C4-type zinc-finger of DNA polymerase delta

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007117)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63960 AT5G63960
fragaria_vesca	FvH4_1g24660 FvH4_1g24660 FvH4_1g24660 FvH4_1g24660 FvH4_1g24660
malus_domestica	MD00G1053300.v1.1 MD05G1053800.v1.1 MD07G1029900.v1.1
prunus_persica	Prupe.4G273600_v2.0.a1 Prupe.4G273600_v2.0.a1 Prupe.4G273600_v2.0.a1
pyrus_communis	pycom01g00060 pycom07g02190
rosa_chinensis	RchiOBHm_Chr2g0122111
rosa_laevigata	RLG00000018650
rosa_multiflora	Rmu_co8081762.1_g000001
rosa_roxburghii	Rroxscaffold_2G00122010
rosa_rugosa	Rorug02G0236500
rosa_samantha	Rh2AG293000 Rh2BG303400 Rh2CG281900 Rh2DG317000
rosa_wichuraiana	Rw2G023500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	110, 1012
AatII	GACGTC	1 cut(s)	114
Acc16I	TGCGCA	1 cut(s)	2456
Acc36I	ACCTGC	1 cut(s)	1476
Acc65I	GGTACC	1 cut(s)	2497
AccB1I	GGYRCC	3 cut(s)	1520, 2497, 2528
AccB7I	CCANNNNNTGG	2 cut(s)	1031, 2486
AccI	GTMKAC	2 cut(s)	102, 1945
AccII	CGCG	1 cut(s)	162
AclWI	GGATC	4 cut(s)	289, 929, 1504, 2499
AcoI	YGGCCR	4 cut(s)	606, 901, 951, 1640
AcuI	CTGAAG	3 cut(s)	162, 1701, 2910
AcyI	GRCGYC	1 cut(s)	111
AdeI	CACNNNGTG	1 cut(s)	1628
AfaI	GTAC	5 cut(s)	788, 1431, 2265, 2499, 2689
AfiI	CCNNNNNNNGG	6 cut(s)	433, 434, 556, 615, 1031, 2486
AflII	CTTAAG	1 cut(s)	1010
AgeI	ACCGGT	1 cut(s)	2910
AjiI	CACGTC	1 cut(s)	75
AjnI	CCWGG	7 cut(s)	452, 945, 1094, 1146, 2221, 2295, 2341
Alw21I	GWGCWC	3 cut(s)	1024, 1962, 2829
Alw26I	GTCTC	4 cut(s)	131, 1201, 2265, 2755
AlwI	GGATC	4 cut(s)	289, 929, 1504, 2499
AoxI	GGCC	9 cut(s)	69, 207, 436, 606, 649, 901, 951, 1608, 1640
ApaI	GGGCCC	2 cut(s)	211, 440
ArsI	GACNNNNNNTTYG	2 cut(s)	360, 392
AsiGI	ACCGGT	1 cut(s)	2910
Asp700I	GAANNNNTTC	2 cut(s)	570, 665
Asp718I	GGTACC	1 cut(s)	2497
AspLEI	GCGC	4 cut(s)	34, 177, 1204, 2457
AspS9I	GGNCC	8 cut(s)	207, 208, 321, 327, 436, 437, 748, 2683
AsuC2I	CCSGG	3 cut(s)	325, 429, 617
AsuHPI	GGTGA	2 cut(s)	190, 1684
AsuII	TTCGAA	1 cut(s)	854
AsuNHI	GCTAGC	1 cut(s)	2552
AvaII	GGWCC	4 cut(s)	321, 327, 748, 2683
BaeGI	GKGCMC	2 cut(s)	211, 440
BalI	TGGCCA	3 cut(s)	608, 953, 1642
BanI	GGYRCC	3 cut(s)	1520, 2497, 2528
BanII	GRGCYC	2 cut(s)	211, 440
BbsI	GAAGAC	2 cut(s)	106, 150
Bbv12I	GWGCWC	3 cut(s)	1024, 1962, 2829
BbvCI	CCTCAGC	1 cut(s)	2019
BccI	CCATC	9 cut(s)	641, 853, 1363, 1600, 1603, 1738, 1841, 2378, 2546
BceAI	ACGGC	2 cut(s)	495, 888
BciT130I	CCWGG	7 cut(s)	454, 947, 1096, 1148, 2223, 2297, 2343
BciVI	GTATCC	1 cut(s)	1662
BclI	TGATCA	1 cut(s)	825
BcnI	CCSGG	3 cut(s)	325, 429, 617
BcoDI	GTCTC	4 cut(s)	131, 1201, 2265, 2755
BfaI	CTAG	4 cut(s)	959, 1278, 1374, 2553
BfmI	CTRYAG	1 cut(s)	1256
BfoI	RGCGCY	2 cut(s)	35, 178
BfrI	CTTAAG	1 cut(s)	1010
BfuAI	ACCTGC	1 cut(s)	1476
BfuI	GTATCC	1 cut(s)	1662
BglII	AGATCT	2 cut(s)	2377, 2585
Bme18I	GGWCC	4 cut(s)	321, 327, 748, 2683
BmgBI	CACGTC	1 cut(s)	75
BmgT120I	GGNCC	8 cut(s)	207, 208, 321, 327, 436, 437, 748, 2683
BmsI	GCATC	8 cut(s)	261, 1035, 1300, 1431, 1592, 2455, 2754, 2824
BmtI	GCTAGC	1 cut(s)	2556
BpiI	GAAGAC	2 cut(s)	106, 150
BpmI	CTGGAG	5 cut(s)	822, 1130, 1692, 1755, 1920
Bpu10I	CCTNAGC	2 cut(s)	921, 2019
Bpu14I	TTCGAA	1 cut(s)	854
BpuEI	CTTGAG	1 cut(s)	491
BpuMI	CCSGG	3 cut(s)	325, 429, 617
BsaHI	GRCGYC	1 cut(s)	111
BsaJI	CCNNGG	7 cut(s)	427, 616, 652, 1095, 2342, 2569, 2803
BsaWI	WCCGGW	1 cut(s)	2910
BsaXI	ACNNNNNCTCC	2 cut(s)	1893, 1923
Bsc4I	CCNNNNNNNGG	6 cut(s)	433, 434, 556, 615, 1031, 2486
Bse118I	RCCGGY	2 cut(s)	43, 2910
Bse1I	ACTGG	4 cut(s)	178, 793, 1903, 2353
Bse3DI	GCAATG	3 cut(s)	691, 805, 2091
BseBI	CCWGG	7 cut(s)	454, 947, 1096, 1148, 2223, 2297, 2343
BseDI	CCNNGG	7 cut(s)	427, 616, 652, 1095, 2342, 2569, 2803
BseGI	GGATG	2 cut(s)	1234, 1774
BseLI	CCNNNNNNNGG	6 cut(s)	433, 434, 556, 615, 1031, 2486
BseMI	GCAATG	3 cut(s)	691, 805, 2091
BseMII	CTCAG	8 cut(s)	912, 1206, 1212, 1491, 2010, 2058, 2388, 2745
BseNI	ACTGG	4 cut(s)	178, 793, 1903, 2353
BseRI	GAGGAG	1 cut(s)	547
BseSI	GKGCMC	2 cut(s)	211, 440
BseYI	CCCAGC	2 cut(s)	55, 2605
BsgI	GTGCAG	4 cut(s)	545, 2297, 2815, 2921
Bsh1236I	CGCG	1 cut(s)	162
BshFI	GGCC	9 cut(s)	71, 209, 438, 608, 651, 903, 953, 1610, 1642
BshNI	GGYRCC	3 cut(s)	1520, 2497, 2528
BshTI	ACCGGT	1 cut(s)	2910
BsiHKAI	GWGCWC	3 cut(s)	1024, 1962, 2829
BsiSI	CCGG	5 cut(s)	44, 324, 429, 616, 2911
BsiWI	CGTACG	1 cut(s)	2263
BslFI	GGGAC	4 cut(s)	61, 166, 734, 2048
BslI	CCNNNNNNNGG	6 cut(s)	433, 434, 556, 615, 1031, 2486
BsmAI	GTCTC	4 cut(s)	131, 1201, 2265, 2755
BsmFI	GGGAC	4 cut(s)	61, 166, 734, 2048
BsmI	GAATGC	4 cut(s)	621, 1273, 2312, 2554
BsnI	GGCC	9 cut(s)	71, 209, 438, 608, 651, 903, 953, 1610, 1642
Bsp119I	TTCGAA	1 cut(s)	854
Bsp120I	GGGCCC	2 cut(s)	207, 436
Bsp1286I	GDGCHC	5 cut(s)	211, 440, 1024, 1962, 2829
Bsp143I	GATC	8 cut(s)	294, 825, 934, 1252, 1496, 2377, 2491, 2585
Bsp19I	CCATGG	1 cut(s)	2569
BspANI	GGCC	9 cut(s)	71, 209, 438, 608, 651, 903, 953, 1610, 1642
BspCNI	CTCAG	8 cut(s)	913, 1207, 1211, 1492, 2011, 2057, 2387, 2746
BspFNI	CGCG	1 cut(s)	162
BspHI	TCATGA	5 cut(s)	669, 708, 1002, 1602, 2385
BspMAI	CTGCAG	1 cut(s)	1260
BspMI	ACCTGC	1 cut(s)	1476
BspOI	GCTAGC	1 cut(s)	2556
BspPI	GGATC	4 cut(s)	289, 929, 1504, 2499
BspQI	GCTCTTC	1 cut(s)	705
BspT104I	TTCGAA	1 cut(s)	854
BspT107I	GGYRCC	3 cut(s)	1520, 2497, 2528
BspTI	CTTAAG	1 cut(s)	1010
BsrDI	GCAATG	3 cut(s)	691, 805, 2091
BsrFI	RCCGGY	2 cut(s)	43, 2910
BsrI	ACTGG	4 cut(s)	178, 793, 1903, 2353
BssAI	RCCGGY	2 cut(s)	43, 2910
BssECI	CCNNGG	7 cut(s)	427, 616, 652, 1095, 2342, 2569, 2803
BssMI	GATC	8 cut(s)	294, 825, 934, 1252, 1496, 2377, 2491, 2585
BssNI	GRCGYC	1 cut(s)	111
BssT1I	CCWWGG	3 cut(s)	652, 2569, 2803
Bst2UI	CCWGG	7 cut(s)	454, 947, 1096, 1148, 2223, 2297, 2343
Bst4CI	ACNGT	9 cut(s)	422, 1117, 1528, 1909, 2074, 2263, 2615, 2687, 2740
Bst6I	CTCTTC	6 cut(s)	524, 705, 1805, 2186, 2263, 2707
BstACI	GRCGYC	1 cut(s)	111
BstAFI	CTTAAG	1 cut(s)	1010
BstAPI	GCANNNNNTGC	1 cut(s)	2528
BstBI	TTCGAA	1 cut(s)	854
BstC8I	GCNNGC	9 cut(s)	69, 211, 215, 610, 901, 1077, 2312, 2459, 2554
BstDSI	CCRYGG	1 cut(s)	2569
BstF5I	GGATG	2 cut(s)	1234, 1774
BstFNI	CGCG	1 cut(s)	162
BstH2I	RGCGCY	2 cut(s)	35, 178
BstHHI	GCGC	4 cut(s)	34, 177, 1204, 2457
BstKTI	GATC	8 cut(s)	297, 828, 937, 1255, 1499, 2380, 2494, 2588
BstMAI	GTCTC	4 cut(s)	131, 1201, 2265, 2755
BstMBI	GATC	8 cut(s)	294, 825, 934, 1252, 1496, 2377, 2491, 2585
BstNI	CCWGG	7 cut(s)	454, 947, 1096, 1148, 2223, 2297, 2343
BstNSI	RCATGY	1 cut(s)	2652
BstSFI	CTRYAG	1 cut(s)	1256
BstSLI	GKGCMC	2 cut(s)	211, 440
BstUI	CGCG	1 cut(s)	162
BstV2I	GAAGAC	2 cut(s)	106, 150
BstX2I	RGATCY	4 cut(s)	294, 1496, 2377, 2585
BstXI	CCANNNNNNTGG	2 cut(s)	392, 1531
BstYI	RGATCY	4 cut(s)	294, 1496, 2377, 2585
BsuI	GTATCC	1 cut(s)	1662
BsuRI	GGCC	9 cut(s)	71, 209, 438, 608, 651, 903, 953, 1610, 1642
BtgI	CCRYGG	1 cut(s)	2569
BtgZI	GCGATG	1 cut(s)	238
BtrI	CACGTC	1 cut(s)	75
BtsCI	GGATG	2 cut(s)	1234, 1774
BtsI	GCAGTG	3 cut(s)	1845, 2701, 2795
BtsIMutI	CAGTG	9 cut(s)	1524, 1845, 1905, 2070, 2125, 2620, 2701, 2745, 2795
BveI	ACCTGC	1 cut(s)	1476
Cac8I	GCNNGC	9 cut(s)	69, 211, 215, 610, 901, 1077, 2312, 2459, 2554
CciI	TCATGA	5 cut(s)	669, 708, 1002, 1602, 2385
CfoI	GCGC	4 cut(s)	34, 177, 1204, 2457
Cfr10I	RCCGGY	2 cut(s)	43, 2910
Cfr13I	GGNCC	8 cut(s)	207, 208, 321, 327, 436, 437, 748, 2683
CpoI	CGGWCCG	1 cut(s)	2683
CseI	GACGC	1 cut(s)	2794
CsiI	ACCWGGT	1 cut(s)	2295
Csp6I	GTAC	5 cut(s)	787, 1430, 2264, 2498, 2688
CspAI	ACCGGT	1 cut(s)	2910
CspCI	CAANNNNNGTGG	2 cut(s)	585, 620
CspI	CGGWCCG	1 cut(s)	2683
CviQI	GTAC	5 cut(s)	787, 1430, 2264, 2498, 2688
DpnI	GATC	8 cut(s)	296, 827, 936, 1254, 1498, 2379, 2493, 2587
DpnII	GATC	8 cut(s)	294, 825, 934, 1252, 1496, 2377, 2491, 2585
DraIII	CACNNNGTG	1 cut(s)	1628
DrdI	GACNNNNNNGTC	2 cut(s)	110, 1012
DseDI	GACNNNNNNGTC	2 cut(s)	110, 1012
EaeI	YGGCCR	4 cut(s)	606, 901, 951, 1640
Eam1104I	CTCTTC	6 cut(s)	524, 705, 1805, 2186, 2263, 2707
EarI	CTCTTC	6 cut(s)	524, 705, 1805, 2186, 2263, 2707
EciI	GGCGGA	2 cut(s)	229, 2696
Eco130I	CCWWGG	3 cut(s)	652, 2569, 2803
Eco24I	GRGCYC	2 cut(s)	211, 440
Eco32I	GATATC	1 cut(s)	1933
Eco47I	GGWCC	4 cut(s)	321, 327, 748, 2683
Eco57I	CTGAAG	3 cut(s)	162, 1701, 2910
EcoO109I	RGGNCCY	2 cut(s)	437, 748
EcoRI	GAATTC	2 cut(s)	95, 1189
EcoRII	CCWGG	7 cut(s)	452, 945, 1094, 1146, 2221, 2295, 2341
EcoRV	GATATC	1 cut(s)	1933
EcoT14I	CCWWGG	3 cut(s)	652, 2569, 2803
EcoT38I	GRGCYC	2 cut(s)	211, 440
ErhI	CCWWGG	3 cut(s)	652, 2569, 2803
FalI	AAGNNNNNCTT	8 cut(s)	135, 167, 241, 273, 2525, 2557, 2655, 2687
FaqI	GGGAC	4 cut(s)	61, 166, 734, 2048
FauI	CCCGC	3 cut(s)	200, 218, 222
FauNDI	CATATG	2 cut(s)	676, 1480
FbaI	TGATCA	1 cut(s)	825
FblI	GTMKAC	2 cut(s)	102, 1945
FokI	GGATG	2 cut(s)	1241, 1761
FriOI	GRGCYC	2 cut(s)	211, 440
FspBI	CTAG	4 cut(s)	959, 1278, 1374, 2553
FspI	TGCGCA	1 cut(s)	2456
GlaI	GCGC	4 cut(s)	33, 176, 1203, 2456
GsaI	CCCAGC	2 cut(s)	59, 2609
GsuI	CTGGAG	5 cut(s)	822, 1130, 1692, 1755, 1920
HaeII	RGCGCY	2 cut(s)	35, 178
HaeIII	GGCC	9 cut(s)	71, 209, 438, 608, 651, 903, 953, 1610, 1642
HapII	CCGG	5 cut(s)	44, 324, 429, 616, 2911
HgaI	GACGC	1 cut(s)	2794
HhaI	GCGC	4 cut(s)	34, 177, 1204, 2457
Hin1I	GRCGYC	1 cut(s)	111
Hin6I	GCGC	4 cut(s)	32, 175, 1202, 2455
HinP1I	GCGC	4 cut(s)	32, 175, 1202, 2455
HincII	GTYRAC	3 cut(s)	478, 1720, 1946
HindII	GTYRAC	3 cut(s)	478, 1720, 1946
HinfI	GANTC	6 cut(s)	560, 1006, 1548, 2042, 2253, 2389
HpaII	CCGG	5 cut(s)	44, 324, 429, 616, 2911
HphI	GGTGA	2 cut(s)	190, 1684
Hpy166II	GTNNAC	8 cut(s)	103, 478, 1234, 1690, 1720, 1896, 1946, 2214
Hpy8I	GTNNAC	8 cut(s)	103, 478, 1234, 1690, 1720, 1896, 1946, 2214
Hpy99I	CGWCG	1 cut(s)	125
HpyCH4III	ACNGT	9 cut(s)	422, 1117, 1528, 1909, 2074, 2263, 2615, 2687, 2740
HpyCH4IV	ACGT	6 cut(s)	25, 74, 111, 999, 1511, 2449
HpySE526I	ACGT	6 cut(s)	25, 74, 111, 999, 1511, 2449
Hsp92I	GRCGYC	1 cut(s)	111
HspAI	GCGC	4 cut(s)	32, 175, 1202, 2455
KpnI	GGTACC	1 cut(s)	2501
Ksp22I	TGATCA	1 cut(s)	825
Kzo9I	GATC	8 cut(s)	294, 825, 934, 1252, 1496, 2377, 2491, 2585
LguI	GCTCTTC	1 cut(s)	705
LmnI	GCTCC	6 cut(s)	61, 868, 1027, 1901, 2482, 2916
LweI	GCATC	8 cut(s)	261, 1035, 1300, 1431, 1592, 2455, 2754, 2824
MabI	ACCWGGT	1 cut(s)	2295
MaeI	CTAG	4 cut(s)	959, 1278, 1374, 2553
MaeII	ACGT	6 cut(s)	25, 74, 111, 999, 1511, 2449
MaeIII	GTNAC	5 cut(s)	355, 961, 1111, 1378, 2576
MalI	GATC	8 cut(s)	296, 827, 936, 1254, 1498, 2379, 2493, 2587
MboI	GATC	8 cut(s)	294, 825, 934, 1252, 1496, 2377, 2491, 2585
MfeI	CAATTG	5 cut(s)	202, 266, 1563, 1856, 2275
MflI	RGATCY	4 cut(s)	294, 1496, 2377, 2585
MhlI	GDGCHC	5 cut(s)	211, 440, 1024, 1962, 2829
MlsI	TGGCCA	3 cut(s)	608, 953, 1642
MluNI	TGGCCA	3 cut(s)	608, 953, 1642
MlyI	GAGTC	2 cut(s)	1000, 2036
MmeI	TCCRAC	1 cut(s)	2819
Mox20I	TGGCCA	3 cut(s)	608, 953, 1642
MroXI	GAANNNNTTC	2 cut(s)	570, 665
MscI	TGGCCA	3 cut(s)	608, 953, 1642
MseI	TTAA	6 cut(s)	1011, 1172, 1749, 1865, 1998, 2514
MslI	CAYNNNNRTG	2 cut(s)	390, 2820
Msp20I	TGGCCA	3 cut(s)	608, 953, 1642
MspA1I	CMGCKG	3 cut(s)	59, 332, 612
MspCI	CTTAAG	1 cut(s)	1010
MspI	CCGG	5 cut(s)	44, 324, 429, 616, 2911
MunI	CAATTG	5 cut(s)	202, 266, 1563, 1856, 2275
Mva1269I	GAATGC	4 cut(s)	621, 1273, 2312, 2554
MvaI	CCWGG	7 cut(s)	454, 947, 1096, 1148, 2223, 2297, 2343
MvnI	CGCG	1 cut(s)	162
NciI	CCSGG	3 cut(s)	325, 429, 617
NcoI	CCATGG	1 cut(s)	2569
NdeI	CATATG	2 cut(s)	676, 1480
NdeII	GATC	8 cut(s)	294, 825, 934, 1252, 1496, 2377, 2491, 2585
NheI	GCTAGC	1 cut(s)	2552
NmuCI	GTSAC	1 cut(s)	1378
NsbI	TGCGCA	1 cut(s)	2456
NspI	RCATGY	1 cut(s)	2652
NspV	TTCGAA	1 cut(s)	854
PagI	TCATGA	5 cut(s)	669, 708, 1002, 1602, 2385
PciSI	GCTCTTC	1 cut(s)	705
PcsI	WCGNNNNNNNCGW	1 cut(s)	117
PctI	GAATGC	4 cut(s)	621, 1273, 2312, 2554
PdmI	GAANNNNTTC	2 cut(s)	570, 665
PfeI	GAWTC	4 cut(s)	560, 1548, 2253, 2389
Pfl23II	CGTACG	1 cut(s)	2263
PflMI	CCANNNNNTGG	2 cut(s)	1031, 2486
PinAI	ACCGGT	1 cut(s)	2910
PleI	GAGTC	2 cut(s)	1000, 2036
PpsI	GAGTC	2 cut(s)	1000, 2036
PpuMI	RGGWCCY	1 cut(s)	748
Psp5II	RGGWCCY	1 cut(s)	748
Psp6I	CCWGG	7 cut(s)	452, 945, 1094, 1146, 2221, 2295, 2341
PspFI	CCCAGC	2 cut(s)	55, 2605
PspGI	CCWGG	7 cut(s)	452, 945, 1094, 1146, 2221, 2295, 2341
PspLI	CGTACG	1 cut(s)	2263
PspOMI	GGGCCC	2 cut(s)	207, 436
PspPI	GGNCC	8 cut(s)	207, 208, 321, 327, 436, 437, 748, 2683
PspPPI	RGGWCCY	1 cut(s)	748
PstI	CTGCAG	1 cut(s)	1260
PsuI	RGATCY	4 cut(s)	294, 1496, 2377, 2585
PvuII	CAGCTG	1 cut(s)	612
RsaI	GTAC	5 cut(s)	788, 1431, 2265, 2499, 2689
RsaNI	GTAC	5 cut(s)	787, 1430, 2264, 2498, 2688
RseI	CAYNNNNRTG	2 cut(s)	390, 2820
Rsr2I	CGGWCCG	1 cut(s)	2683
RsrII	CGGWCCG	1 cut(s)	2683
SalI	GTCGAC	1 cut(s)	1944
SapI	GCTCTTC	1 cut(s)	705
SaqAI	TTAA	6 cut(s)	1011, 1172, 1749, 1865, 1998, 2514
Sau3AI	GATC	8 cut(s)	294, 825, 934, 1252, 1496, 2377, 2491, 2585
Sau96I	GGNCC	8 cut(s)	207, 208, 321, 327, 436, 437, 748, 2683
SchI	GAGTC	2 cut(s)	1000, 2036
SduI	GDGCHC	5 cut(s)	211, 440, 1024, 1962, 2829
SexAI	ACCWGGT	1 cut(s)	2295
SfaNI	GCATC	8 cut(s)	261, 1035, 1300, 1431, 1592, 2455, 2754, 2824
SfcI	CTRYAG	1 cut(s)	1256
SfuI	TTCGAA	1 cut(s)	854
SinI	GGWCC	4 cut(s)	321, 327, 748, 2683
SmiMI	CAYNNNNRTG	2 cut(s)	390, 2820
SmlI	CTYRAG	2 cut(s)	470, 1010
SmoI	CTYRAG	2 cut(s)	470, 1010
SspMI	CTAG	4 cut(s)	959, 1278, 1374, 2553
StyI	CCWWGG	3 cut(s)	652, 2569, 2803
TaaI	ACNGT	9 cut(s)	422, 1117, 1528, 1909, 2074, 2263, 2615, 2687, 2740
TaiI	ACGT	6 cut(s)	28, 77, 114, 1002, 1514, 2452
TaqI	TCGA	9 cut(s)	120, 135, 168, 289, 854, 1945, 2148, 2232, 2256
TauI	GCSGC	2 cut(s)	37, 50
TfiI	GAWTC	4 cut(s)	560, 1548, 2253, 2389
Tru1I	TTAA	6 cut(s)	1011, 1172, 1749, 1865, 1998, 2514
Tru9I	TTAA	6 cut(s)	1011, 1172, 1749, 1865, 1998, 2514
TscAI	CASTG	9 cut(s)	1531, 1845, 1912, 2077, 2125, 2620, 2708, 2745, 2795
TseFI	GTSAC	1 cut(s)	1378
Tsp45I	GTSAC	1 cut(s)	1378
TspGWI	ACGGA	1 cut(s)	1009
TspRI	CASTG	9 cut(s)	1531, 1845, 1912, 2077, 2125, 2620, 2708, 2745, 2795
Van91I	CCANNNNNTGG	2 cut(s)	1031, 2486
Vha464I	CTTAAG	1 cut(s)	1010
VpaK11BI	GGWCC	4 cut(s)	321, 327, 748, 2683
XceI	RCATGY	1 cut(s)	2652
XmiI	GTMKAC	2 cut(s)	102, 1945
XmnI	GAANNNNTTC	2 cut(s)	570, 665
XspI	CTAG	4 cut(s)	959, 1278, 1374, 2553
ZraI	GACGTC	1 cut(s)	112

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.