AT5G64070

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012264
Physical Location & Seq
Reverse (-)
25636882 .. 25644478
7597 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64070.2

Sequence Viewer

Length: 3366 bp
ATGCCGATGGGACGCTTTCTATCTTTGGTCCGAGGAGACTCGGCTGAGTCACCTCGTGAGATTACCTCACAGAGTAATATCATCGGCGATACTGGTTCTAATGGTTGGCTTATTAGGTTTTTCGATTCTGCTTTCTTCTGTGAGTGGATTGCTGTTAGTTATCTGTACAAGCACCCTCATGCTGGTGTTAGGGATTACTTATGCAATAGGATGTATACGCTTCCCTTATCAGGTATTGAGAGTTATCTCTTCCAGATTTGCTACATGATGGTGCACAAACCAAGCCCATCGCTTGATAAGTTTGTTATAGACATCTGTGGAAAGTCACTGAAAATCGCACTGAAAGTGCATTGGTTTTTGTTGGCTGAGCTGGAGGATGCTGACGATAATGAAGGCATCAGCAGGATTCAAGAGAAGTGTCAAATTGCAGCTACTTTGATGGGTGAGTGGTCTCCTCTCATGCGTCCACAGAACGAGGTCTCAACCCCAGGGAGCAAGAATCAGGTCCTAAATAGACTTTTGTCATCAAAACAGAAGCTCTTCTCCTTAAAGCTATCTCCTCCCACCCAGAAGTCTTTGTCATTCTCACCTTCGCCAGGGACCAACGTGCAGGATGATGGTAGTCAGTTACCTGCTGAGGACAATAAGATTTTTAAGAAACTTATCCCAAGTCCTAAAGTCAGAGATGCTTTAATGTTTAGAAAGTCAGCTGACAAAGATGATGAAGAGAGTGAAAAGGAAGGATTCTTCAAGAGGCTCTTGAGGGACAGCAAAGGTGAGGGTGATGAACCAATACCTAACTCAGAGGGTTTCTTTAAGCGCCTTTTGAAAGACAATAAATCAGAAGATGAGGACATAACAAACAGTTCCGAGGGGTTCTTCAAGAGGCTCTTGAGTAGTAAAGGAGAAAGCGAGGAATTGACATCGAGTTCAGATGGGCTGTTTAAAAGGTTGTTACGAGATAATAAGGGAGATGAAGAGGAATTGGGTGCAAATTCAGACAGTTTCTTCAAGAGATTGTTACGGGAGAGTAAAAACGAGGACGAGGAGTCAAACCCAAACTCAGAAGGTTTTTTTAAGAAATTATTCCGTGACAGCAAACCTGAAGATGATAAAGTTCCTAAAGAAGTGGATGATGAGGACAAAGATGGGTTCCTTAAGAAACTTTTCAGGGAAAAAAATGATGACAAAAGACATGGTAGTGAAAAGAATGAAGCCAATGGAACAGTGTATGCCGATAAGAAATCTGGTGAAGAGGATGAGAGGGAGGGGTTTTTCAAAAAATTCTTTAAGGAAAAATCTGATGACAAGAAAGACATTGTCAAGGTTGATGACGGGAATGAAAGTGAGGGTGATGAATCTCCAGAATTTTCTCTTTTCAAAAGATTATTCCGTATACATCCCGAAGATGCAAAACCTACTTCAGAAAATGAAAATAGCAGCAATGGCTTAGTTGAAAGCAGTCCTGGGACAGAAAACTTTTTCCGCAAGTTGTTCAGAGATCGTGACCAATCTGTTGAAGATTCTGAACTGTTTGGATCAAAGAAACACAAGGAGAAACGGCCAGGCTCACCTAAACAGCGGGATGATACTCCATCTGGTAAGCCCCCGCTACCAAACAATACTGCATCACAATTCAGGAAAGGGGCTTACCACGAGTCGTTGGAGTTTGTACAAGCGTTATGTGAAACGTCATATGGTTTGGTAGATATCTTTCCCATCGAAGATCGGAAGATTGGTCTTCGGGAGTCTCTTGCAGAGATCAATTTTCATTTATCGGAAGCTGAAATCACTGGAGGAATTTGTTTTCCAATGGGGAGAGGAGTTTTTCGTGTGGTCCATATTCCTGAAGACGAATGCATTCTTTTGAATTCTAGGGAAAAGGCGCCATACATGATCTCCGTAGAAGTTTTGAAAGCAGAAACACCCAGTGCTAAAGAATCATCGAACTCCCAGAAGCTTTCTAAAGGGGGGATTCCATTGGCAAACGGGGACGCATTCTTGCAAAAGCCACCTCCATGGGCTTATCCACTATGGACTACTCAGGAGGTTTATCGCAACAGTGCTGACCGAATGTCGTTATCCACTGCTCAAGCAATTGATCAAGCGATGACTCCTAAGTCAGAGGTGAAGGTGAAACTTGTCAATGTAAGTCTCTCAGTGGAGGATCGTACTTCAGCTCTTGAATCATTTGGCGATCCAATTGATGATGTTCTGGGGGAAGCCCCAAGGACTGGGCTGAACAATGATCTTGAATGGGTAAGGGTGGTCGTAACGGCTGACCCAGGACTTAGAATGGAAAGCATTCCTGATCCATCAGTCCCACGTAAAAAGGAACATCGTCGTGTTCCAAGTACTGTTGCCATGGAGGAAGTTAGGGCTGCTGCAGCGAAGGGAGAGGCACCTCCAGGCCTTCCTCTCAAAGGGGCGGGTCAGGATTCGTCGGATGCACAACCAAGGGCCAATGGTGGAATGCTGAAGGAAGGTGATGCCTTATCAGGTGAACTTTGGGAGGGAAAGCGAGACAGAATACGTAAAGCTTCAATATATGGAAAGTTACCTGGTTGGGACCTGCGTTCTATCATTGTGAAGAGCGGTGACGACTGTAGGCAGGAGCATCTTGCTGTGCAACTCATTTCTCACTTTTATGATATATTCCAGGAAGCAGGTCTACCCCTCTGGTTACGTCCTTATGAAGTCTTGGTTACATCTTCTTATACTGCCCTTATAGAAACAATTCCAGATACGGCTTCTATTCATTCTATCAAAAGTAGATATCCCAACATAACGAGCCTACGTGATTTCTTTGTTGCAAAGTATAAAGAAAACTCTCCAAGTTTTAAGCTCGCCCAGAGGAATTTCGTGGAAAGTATGGCCGGATATTCTTTGGTCTGTTACCTTCTGCAGGTAAAAGATCGTCATAATGGAAATCTTCTCTTGGATGAAGAGGGTCACATTATACACATTGATTTTGGTTTTATGCTTTCGAATTCTCCTGGTGGCGTAAACTTTGAGAGTGCCCCGTTTAAGTTAACCCGTGAACTTCTTGAGGTCATGGACTCTGATGCTGATGGGGTTCCAAGTGAGTTCTTTGACTATTTCAAGGTGCTATGCATTCAAGGATTCCTTACATGTAGAAAGCACGCGGAACGGATTATTCTCTTAGTTGAAATGTTACAGGATTCGGGTTTCCCTTGCTTCAAAGGTGGTCCACGAACCATTCAAAACCTAAGAAAAAGATTCCATCTAAGTTTAACTGAAGAGCAATGTGTCTCGTTGGTCCTCTCTCTCATCAGCAGTAGCTTAGATGCTTGGCGGACACGACAATATGATTACTACCAACGGGTCTTAAATGGAATATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012506 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017137 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0030258 GO:0030659 GO:0031090 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0035618 GO:0035619 GO:0040007 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044706 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048015 GO:0048017 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051704 GO:0051716 GO:0052742 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0097708 GO:0099402 GO:0120025 GO:0120038 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

1121

Amino Acids

126.37

Weight (kDa)

5.47

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI4KB-PIK1_PIK PF21245 50 - 120 5.4e-11 PI4KB/PIK1, accessory (PIK) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09350 AT5G64070 AT5G64070
fragaria_vesca FvH4_1g24240
malus_domestica MD09G1261800.v1.1 MD17G1256600.v1.1
prunus_persica Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1
pyrus_communis pycom09g17610 pycom17g25880
rosa_chinensis RchiOBHm_Chr2g0120731
rosa_laevigata RLG00000018575
rosa_multiflora Rmu_sc0000319.1_g000011
rosa_roxburghii Rroxscaffold_2G00123200
rosa_rugosa Rorug02G0230500
rosa_samantha Rh2BG298500 Rh2CG276200
rosa_wichuraiana Rw2G023030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2119
Acc36I ACCTGC 4 cut(s) 640, 2580, 2657, 2896
AccB1I GGYRCC 2 cut(s) 1885, 2401
AccB7I CCANNNNNTGG 1 cut(s) 2234
AccBSI CCGCTC 1 cut(s) 2595
AccI GTMKAC 3 cut(s) 215, 1396, 2671
AccII CGCG 1 cut(s) 3146
AciI CCGC 7 cut(s) 1486, 1582, 1610, 2429, 2595, 3146, 3316
AclWI GGATC 4 cut(s) 1546, 2175, 2192, 2306
AcoI YGGCCR 2 cut(s) 1562, 2874
AcsI RAATTY 7 cut(s) 994, 1283, 1367, 1800, 1870, 2857, 2989
AcuI CTGAAG 6 cut(s) 1125, 1407, 1869, 2160, 2498, 3279
AcyI GRCGYC 1 cut(s) 1886
AdeI CACNNNGTG 2 cut(s) 56, 1931
AfaI GTAC 4 cut(s) 167, 1674, 2173, 2356
AfiI CCNNNNNNNGG 6 cut(s) 182, 230, 596, 2234, 2423, 2905
AflII CTTAAG 1 cut(s) 1157
AflIII ACRYGT 1 cut(s) 3131
AhdI GACNNNNNGTC 2 cut(s) 1048, 2074
AjnI CCWGG 9 cut(s) 487, 595, 1465, 1564, 2284, 2407, 2560, 2658, 2995
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 7 cut(s) 30, 456, 484, 1755, 2159, 2517, 3277
Alw44I GTGCAC 1 cut(s) 272
AlwI GGATC 4 cut(s) 1546, 2175, 2192, 2306
AoxI GGCC 4 cut(s) 1562, 2410, 2460, 2874
ApaLI GTGCAC 1 cut(s) 272
ApeKI GCWGC 5 cut(s) 428, 1440, 2381, 2384, 2387
ApoI RAATTY 7 cut(s) 994, 1283, 1367, 1800, 1870, 2857, 2989
Asp700I GAANNNNTTC 6 cut(s) 539, 1085, 1166, 1860, 2304, 2736
AspLEI GCGC 2 cut(s) 822, 1888
AspS9I GGNCC 8 cut(s) 28, 505, 600, 1837, 2460, 2569, 3209, 3280
AsuII TTCGAA 1 cut(s) 2987
AvaII GGWCC 7 cut(s) 28, 505, 600, 1837, 2569, 3209, 3280
BaeGI GKGCMC 2 cut(s) 276, 3022
BanI GGYRCC 2 cut(s) 1885, 2401
BarI GAAGNNNNNNTAC 2 cut(s) 2655, 2687
BauI CACGAG 2 cut(s) 54, 1655
BbsI GAAGAC 2 cut(s) 1733, 1857
Bbv12I GWGCWC 1 cut(s) 276
BbvCI CCTCAGC 1 cut(s) 636
BbvI GCAGC 5 cut(s) 440, 1452, 2368, 2371, 2399
BceAI ACGGC 3 cut(s) 1577, 2292, 2763
BciT130I CCWGG 9 cut(s) 489, 597, 1467, 1566, 2286, 2409, 2562, 2660, 2997
BclI TGATCA 1 cut(s) 2101
BcoDI GTCTC 7 cut(s) 30, 456, 484, 1755, 2159, 2517, 3277
BfaI CTAG 1 cut(s) 1875
BfmI CTRYAG 3 cut(s) 2385, 2605, 2903
BfoI RGCGCY 2 cut(s) 823, 1889
BfrI CTTAAG 1 cut(s) 1157
BfuAI ACCTGC 4 cut(s) 640, 2580, 2657, 2896
BisI GCNGC 5 cut(s) 429, 1441, 2382, 2385, 2388
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 5 cut(s) 430, 1442, 2383, 2386, 2389
BmcAI AGTACT 1 cut(s) 2356
Bme1390I CCNGG 9 cut(s) 489, 597, 1467, 1566, 2286, 2409, 2562, 2660, 2997
Bme18I GGWCC 7 cut(s) 28, 505, 600, 1837, 2569, 3209, 3280
BmeRI GACNNNNNGTC 2 cut(s) 1048, 2074
BmgT120I GGNCC 8 cut(s) 28, 505, 600, 1837, 2460, 2569, 3209, 3280
BmiI GGNNCC 6 cut(s) 601, 1154, 1887, 2403, 2570, 3078
BmrFI CCNGG 9 cut(s) 489, 597, 1467, 1566, 2286, 2409, 2562, 2660, 2997
BmrI ACTGGG 2 cut(s) 1923, 2244
BmuI ACTGGG 2 cut(s) 1923, 2244
BoxI GACNNNNGTC 1 cut(s) 520
BpiI GAAGAC 2 cut(s) 1733, 1857
BplI GAGNNNNNCTC 2 cut(s) 50, 82
BpmI CTGGAG 4 cut(s) 392, 1347, 1815, 2391
Bpu10I CCTNAGC 1 cut(s) 636
Bpu1102I GCTNAGC 1 cut(s) 366
Bpu14I TTCGAA 1 cut(s) 2987
BpuEI CTTGAG 4 cut(s) 781, 913, 2076, 3068
BsaAI YACGTR 3 cut(s) 2327, 2534, 2798
BsaHI GRCGYC 1 cut(s) 1886
BsaI GGTCTC 2 cut(s) 456, 484
BsaXI ACNNNNNCTCC 2 cut(s) 1883, 1913
Bsc4I CCNNNNNNNGG 6 cut(s) 182, 230, 596, 2234, 2423, 2905
Bse1I ACTGG 4 cut(s) 97, 1798, 1929, 2239
Bse3DI GCAATG 2 cut(s) 1450, 3272
BseBI CCWGG 9 cut(s) 489, 597, 1467, 1566, 2286, 2409, 2562, 2660, 2997
BseGI GGATG 9 cut(s) 216, 382, 619, 1138, 1264, 1399, 1591, 2452, 2947
BseLI CCNNNNNNNGG 6 cut(s) 182, 230, 596, 2234, 2423, 2905
BseMI GCAATG 2 cut(s) 1450, 3272
BseMII CTCAG 7 cut(s) 36, 357, 627, 816, 1077, 2057, 2172
BseNI ACTGG 4 cut(s) 97, 1798, 1929, 2239
BseRI GAGGAG 5 cut(s) 48, 444, 549, 1061, 1836
BseSI GKGCMC 2 cut(s) 276, 3022
BseXI GCAGC 5 cut(s) 440, 1452, 2368, 2371, 2399
BsgI GTGCAG 1 cut(s) 629
Bsh1236I CGCG 1 cut(s) 3146
BshFI GGCC 4 cut(s) 1564, 2412, 2462, 2876
BshNI GGYRCC 2 cut(s) 1885, 2401
BsiHKAI GWGCWC 1 cut(s) 276
BsiSI CCGG 1 cut(s) 2877
BslFI GGGAC 7 cut(s) 24, 613, 779, 1483, 2006, 2306, 2582
BslI CCNNNNNNNGG 6 cut(s) 182, 230, 596, 2234, 2423, 2905
BsmAI GTCTC 7 cut(s) 30, 456, 484, 1755, 2159, 2517, 3277
BsmFI GGGAC 7 cut(s) 24, 613, 779, 1483, 2006, 2306, 2582
BsmI GAATGC 6 cut(s) 1860, 1862, 1997, 2304, 2478, 3114
BsnI GGCC 4 cut(s) 1564, 2412, 2462, 2876
Bso31I GGTCTC 2 cut(s) 456, 484
Bsp119I TTCGAA 1 cut(s) 2987
Bsp1286I GDGCHC 2 cut(s) 276, 3022
Bsp1407I TGTACA 2 cut(s) 165, 1672
Bsp1720I GCTNAGC 1 cut(s) 366
Bsp19I CCATGG 2 cut(s) 2018, 2364
BspACI CCGC 7 cut(s) 1486, 1582, 1610, 2429, 2595, 3146, 3316
BspANI GGCC 4 cut(s) 1564, 2412, 2462, 2876
BspCNI CTCAG 7 cut(s) 37, 358, 628, 815, 1076, 2056, 2171
BspFNI CGCG 1 cut(s) 3146
BspLI GGNNCC 6 cut(s) 601, 1154, 1887, 2403, 2570, 3078
BspMAI CTGCAG 2 cut(s) 2389, 2907
BspMI ACCTGC 4 cut(s) 640, 2580, 2657, 2896
BspPI GGATC 4 cut(s) 1546, 2175, 2192, 2306
BspQI GCTCTTC 3 cut(s) 545, 2585, 3255
BspT104I TTCGAA 1 cut(s) 2987
BspT107I GGYRCC 2 cut(s) 1885, 2401
BspTI CTTAAG 1 cut(s) 1157
BspTNI GGTCTC 2 cut(s) 456, 484
BsrBI CCGCTC 1 cut(s) 2595
BsrDI GCAATG 2 cut(s) 1450, 3272
BsrGI TGTACA 2 cut(s) 165, 1672
BsrI ACTGG 4 cut(s) 97, 1798, 1929, 2239
BssNAI GTATAC 2 cut(s) 216, 1397
BssNI GRCGYC 1 cut(s) 1886
BssSI CACGAG 2 cut(s) 54, 1655
BssT1I CCWWGG 4 cut(s) 2018, 2228, 2364, 2456
Bst1107I GTATAC 2 cut(s) 216, 1397
Bst2BI CACGAG 2 cut(s) 54, 1655
Bst2UI CCWGG 9 cut(s) 489, 597, 1467, 1566, 2286, 2409, 2562, 2660, 2997
Bst4CI ACNGT 7 cut(s) 866, 1004, 1228, 1533, 2063, 2359, 2606
Bst6I CTCTTC 8 cut(s) 254, 545, 720, 972, 1248, 2585, 2940, 3255
BstACI GRCGYC 1 cut(s) 1886
BstAFI CTTAAG 1 cut(s) 1157
BstAUI TGTACA 2 cut(s) 165, 1672
BstBAI YACGTR 3 cut(s) 2327, 2534, 2798
BstBI TTCGAA 1 cut(s) 2987
BstC8I GCNNGC 2 cut(s) 2847, 3144
BstDSI CCRYGG 2 cut(s) 2018, 2364
BstENI CCTNNNNNAGG 3 cut(s) 594, 2421, 2903
BstF5I GGATG 9 cut(s) 216, 382, 619, 1138, 1264, 1399, 1591, 2452, 2947
BstFNI CGCG 1 cut(s) 3146
BstH2I RGCGCY 2 cut(s) 823, 1889
BstHHI GCGC 2 cut(s) 822, 1888
BstMAI GTCTC 7 cut(s) 30, 456, 484, 1755, 2159, 2517, 3277
BstMWI GCNNNNNNNGC 2 cut(s) 1446, 2387
BstNI CCWGG 9 cut(s) 489, 597, 1467, 1566, 2286, 2409, 2562, 2660, 2997
BstNSI RCATGY 1 cut(s) 3135
BstPAI GACNNNNGTC 1 cut(s) 520
BstSCI CCNGG 9 cut(s) 487, 595, 1465, 1564, 2284, 2407, 2560, 2658, 2995
BstSFI CTRYAG 3 cut(s) 2385, 2605, 2903
BstSLI GKGCMC 2 cut(s) 276, 3022
BstSNI TACGTA 1 cut(s) 2534
BstUI CGCG 1 cut(s) 3146
BstV1I GCAGC 5 cut(s) 440, 1452, 2368, 2371, 2399
BstV2I GAAGAC 2 cut(s) 1733, 1857
BstXI CCANNNNNNTGG 1 cut(s) 2019
BstZ17I GTATAC 2 cut(s) 216, 1397
BsuRI GGCC 4 cut(s) 1564, 2412, 2462, 2876
BtgI CCRYGG 2 cut(s) 2018, 2364
BtgZI GCGATG 2 cut(s) 273, 2123
BtsCI GGATG 9 cut(s) 216, 382, 619, 1138, 1264, 1399, 1591, 2452, 2947
BtsI GCAGTG 1 cut(s) 2085
BtsIMutI CAGTG 8 cut(s) 326, 338, 1233, 1791, 1936, 2068, 2085, 2166
BveI ACCTGC 4 cut(s) 640, 2580, 2657, 2896
Cac8I GCNNGC 2 cut(s) 2847, 3144
CfoI GCGC 2 cut(s) 822, 1888
Cfr13I GGNCC 8 cut(s) 28, 505, 600, 1837, 2460, 2569, 3209, 3280
CseI GACGC 3 cut(s) 21, 452, 2003
CsiI ACCWGGT 1 cut(s) 2560
Csp6I GTAC 4 cut(s) 166, 1673, 2172, 2355
CviAII CATG 9 cut(s) 179, 265, 460, 1196, 1894, 2019, 2365, 3055, 3132
CviQI GTAC 4 cut(s) 166, 1673, 2172, 2355
DinI GGCGCC 1 cut(s) 1887
DraI TTTAAA 1 cut(s) 946
DraIII CACNNNGTG 2 cut(s) 56, 1931
DrdI GACNNNNNNGTC 1 cut(s) 2119
DriI GACNNNNNGTC 2 cut(s) 1048, 2074
DseDI GACNNNNNNGTC 1 cut(s) 2119
EaeI YGGCCR 2 cut(s) 1562, 2874
Eam1104I CTCTTC 8 cut(s) 254, 545, 720, 972, 1248, 2585, 2940, 3255
Eam1105I GACNNNNNGTC 2 cut(s) 1048, 2074
EarI CTCTTC 8 cut(s) 254, 545, 720, 972, 1248, 2585, 2940, 3255
EciI GGCGGA 1 cut(s) 3331
Eco105I TACGTA 1 cut(s) 2534
Eco130I CCWWGG 4 cut(s) 2018, 2228, 2364, 2456
Eco147I AGGCCT 1 cut(s) 2412
Eco31I GGTCTC 2 cut(s) 456, 484
Eco32I GATATC 2 cut(s) 1711, 2777
Eco47I GGWCC 7 cut(s) 28, 505, 600, 1837, 2569, 3209, 3280
Eco57I CTGAAG 6 cut(s) 1125, 1407, 1869, 2160, 2498, 3279
EcoNI CCTNNNNNAGG 3 cut(s) 594, 2421, 2903
EcoO109I RGGNCCY 2 cut(s) 505, 2569
EcoRI GAATTC 2 cut(s) 1870, 2989
EcoRII CCWGG 9 cut(s) 487, 595, 1465, 1564, 2284, 2407, 2560, 2658, 2995
EcoRV GATATC 2 cut(s) 1711, 2777
EcoT14I CCWWGG 4 cut(s) 2018, 2228, 2364, 2456
EcoT22I ATGCAT 2 cut(s) 1862, 3116
EgeI GGCGCC 1 cut(s) 1887
EheI GGCGCC 1 cut(s) 1887
ErhI CCWWGG 4 cut(s) 2018, 2228, 2364, 2456
FaeI CATG 9 cut(s) 182, 268, 463, 1199, 1897, 2022, 2368, 3058, 3135
FalI AAGNNNNNCTT 6 cut(s) 743, 775, 875, 907, 3111, 3143
FaqI GGGAC 7 cut(s) 24, 613, 779, 1483, 2006, 2306, 2582
FatI CATG 9 cut(s) 178, 264, 459, 1195, 1893, 2018, 2364, 3054, 3131
FauI CCCGC 3 cut(s) 1575, 1617, 2422
FauNDI CATATG 1 cut(s) 1696
FbaI TGATCA 1 cut(s) 2101
FblI GTMKAC 3 cut(s) 215, 1396, 2671
Fnu4HI GCNGC 5 cut(s) 429, 1441, 2382, 2385, 2388
FokI GGATG 9 cut(s) 223, 389, 626, 1145, 1271, 1386, 1598, 2459, 2954
Fsp4HI GCNGC 5 cut(s) 429, 1441, 2382, 2385, 2388
FspBI CTAG 1 cut(s) 1875
GlaI GCGC 2 cut(s) 821, 1887
GluI GCNGC 5 cut(s) 429, 1441, 2382, 2385, 2388
GsuI CTGGAG 4 cut(s) 392, 1347, 1815, 2391
HaeII RGCGCY 2 cut(s) 823, 1889
HaeIII GGCC 4 cut(s) 1564, 2412, 2462, 2876
HapII CCGG 1 cut(s) 2877
HgaI GACGC 3 cut(s) 21, 452, 2003
HhaI GCGC 2 cut(s) 822, 1888
Hin1I GRCGYC 1 cut(s) 1886
Hin1II CATG 9 cut(s) 182, 268, 463, 1199, 1897, 2022, 2368, 3058, 3135
Hin6I GCGC 2 cut(s) 820, 1886
HinP1I GCGC 2 cut(s) 820, 1886
HincII GTYRAC 1 cut(s) 3033
HindII GTYRAC 1 cut(s) 3033
HindIII AAGCTT 2 cut(s) 1958, 2538
HpaI GTTAAC 1 cut(s) 3033
HpaII CCGG 1 cut(s) 2877
Hpy99I CGWCG 2 cut(s) 2346, 2446
HpyCH4III ACNGT 7 cut(s) 866, 1004, 1228, 1533, 2063, 2359, 2606
HpyCH4IV ACGT 6 cut(s) 606, 1691, 2326, 2533, 2686, 2797
HpyF10VI GCNNNNNNNGC 2 cut(s) 1446, 2387
HpySE526I ACGT 6 cut(s) 606, 1691, 2326, 2533, 2686, 2797
Hsp92I GRCGYC 1 cut(s) 1886
Hsp92II CATG 9 cut(s) 182, 268, 463, 1199, 1897, 2022, 2368, 3058, 3135
HspAI GCGC 2 cut(s) 820, 1886
KasI GGCGCC 1 cut(s) 1885
Ksp22I TGATCA 1 cut(s) 2101
KspAI GTTAAC 1 cut(s) 3033
LguI GCTCTTC 3 cut(s) 545, 2585, 3255
LmnI GCTCC 2 cut(s) 492, 2614
Lsp1109I GCAGC 5 cut(s) 440, 1452, 2368, 2371, 2399
MabI ACCWGGT 1 cut(s) 2560
MaeI CTAG 1 cut(s) 1875
MaeII ACGT 6 cut(s) 606, 1691, 2326, 2533, 2686, 2797
MbiI CCGCTC 1 cut(s) 2595
MfeI CAATTG 2 cut(s) 2097, 2202
MhlI GDGCHC 2 cut(s) 276, 3022
Mly113I GGCGCC 1 cut(s) 1886
MlyI GAGTC 7 cut(s) 32, 56, 1058, 1667, 1757, 2107, 3053
MmeI TCCRAC 2 cut(s) 1644, 2424
Mph1103I ATGCAT 2 cut(s) 1862, 3116
MroXI GAANNNNTTC 6 cut(s) 539, 1085, 1166, 1860, 2304, 2736
MslI CAYNNNNRTG 7 cut(s) 177, 183, 269, 1200, 2017, 2343, 2646
MspA1I CMGCKG 2 cut(s) 710, 1582
MspCI CTTAAG 1 cut(s) 1157
MspI CCGG 1 cut(s) 2877
MspR9I CCNGG 9 cut(s) 489, 597, 1467, 1566, 2286, 2409, 2562, 2660, 2997
MunI CAATTG 2 cut(s) 2097, 2202
Mva1269I GAATGC 6 cut(s) 1860, 1862, 1997, 2304, 2478, 3114
MvaI CCWGG 9 cut(s) 489, 597, 1467, 1566, 2286, 2409, 2562, 2660, 2997
MvnI CGCG 1 cut(s) 3146
MwoI GCNNNNNNNGC 2 cut(s) 1446, 2387
NarI GGCGCC 1 cut(s) 1886
NcoI CCATGG 2 cut(s) 2018, 2364
NdeI CATATG 1 cut(s) 1696
NlaIII CATG 9 cut(s) 182, 268, 463, 1199, 1897, 2022, 2368, 3058, 3135
NlaIV GGNNCC 6 cut(s) 601, 1154, 1887, 2403, 2570, 3078
NmeAIII GCCGAG 1 cut(s) 20
NmuCI GTSAC 6 cut(s) 48, 324, 1091, 1505, 2597, 2951
NsiI ATGCAT 2 cut(s) 1862, 3116
NspI RCATGY 1 cut(s) 3135
NspV TTCGAA 1 cut(s) 2987
PasI CCCWGGG 1 cut(s) 488
PceI AGGCCT 1 cut(s) 2412
PciI ACATGT 1 cut(s) 3131
PciSI GCTCTTC 3 cut(s) 545, 2585, 3255
PctI GAATGC 6 cut(s) 1860, 1862, 1997, 2304, 2478, 3114
PdmI GAANNNNTTC 6 cut(s) 539, 1085, 1166, 1860, 2304, 2736
PflFI GACNNNGTC 1 cut(s) 1319
PflMI CCANNNNNTGG 1 cut(s) 2234
PfoI TCCNGGA 1 cut(s) 2658
PkrI GCNGC 5 cut(s) 430, 1442, 2383, 2386, 2389
PleI GAGTC 7 cut(s) 32, 55, 1057, 1666, 1756, 2107, 3053
PluTI GGCGCC 1 cut(s) 1889
PpsI GAGTC 7 cut(s) 32, 55, 1057, 1666, 1756, 2107, 3053
Ppu21I YACGTR 3 cut(s) 2327, 2534, 2798
PpuMI RGGWCCY 2 cut(s) 505, 2569
PscI ACATGT 1 cut(s) 3131
PshAI GACNNNNGTC 1 cut(s) 520
Psp5II RGGWCCY 2 cut(s) 505, 2569
Psp6I CCWGG 9 cut(s) 487, 595, 1465, 1564, 2284, 2407, 2560, 2658, 2995
PspGI CCWGG 9 cut(s) 487, 595, 1465, 1564, 2284, 2407, 2560, 2658, 2995
PspN4I GGNNCC 6 cut(s) 601, 1154, 1887, 2403, 2570, 3078
PspPI GGNCC 8 cut(s) 28, 505, 600, 1837, 2460, 2569, 3209, 3280
PspPPI RGGWCCY 2 cut(s) 505, 2569
PstI CTGCAG 2 cut(s) 2389, 2907
PsyI GACNNNGTC 1 cut(s) 1319
PvuII CAGCTG 1 cut(s) 710
RsaI GTAC 4 cut(s) 167, 1674, 2173, 2356
RsaNI GTAC 4 cut(s) 166, 1673, 2172, 2355
RseI CAYNNNNRTG 7 cut(s) 177, 183, 269, 1200, 2017, 2343, 2646
SapI GCTCTTC 3 cut(s) 545, 2585, 3255
SatI GCNGC 5 cut(s) 429, 1441, 2382, 2385, 2388
Sau96I GGNCC 8 cut(s) 28, 505, 600, 1837, 2460, 2569, 3209, 3280
ScaI AGTACT 1 cut(s) 2356
SchI GAGTC 7 cut(s) 32, 56, 1058, 1667, 1757, 2107, 3053
ScrFI CCNGG 9 cut(s) 489, 597, 1467, 1566, 2286, 2409, 2562, 2660, 2997
SduI GDGCHC 2 cut(s) 276, 3022
SexAI ACCWGGT 1 cut(s) 2560
SfcI CTRYAG 3 cut(s) 2385, 2605, 2903
SfoI GGCGCC 1 cut(s) 1887
SfuI TTCGAA 1 cut(s) 2987
SinI GGWCC 7 cut(s) 28, 505, 600, 1837, 2569, 3209, 3280
SmiMI CAYNNNNRTG 7 cut(s) 177, 183, 269, 1200, 2017, 2343, 2646
SmlI CTYRAG 5 cut(s) 760, 892, 1157, 2091, 3047
SmoI CTYRAG 5 cut(s) 760, 892, 1157, 2091, 3047
SnaBI TACGTA 1 cut(s) 2534
SseBI AGGCCT 1 cut(s) 2412
SsiI CCGC 7 cut(s) 1486, 1582, 1610, 2429, 2595, 3146, 3316
SspDI GGCGCC 1 cut(s) 1885
SspI AATATT 1 cut(s) 3360
SspMI CTAG 1 cut(s) 1875
StuI AGGCCT 1 cut(s) 2412
StyD4I CCNGG 9 cut(s) 487, 595, 1465, 1564, 2284, 2407, 2560, 2658, 2995
StyI CCWWGG 4 cut(s) 2018, 2228, 2364, 2456
TaaI ACNGT 7 cut(s) 866, 1004, 1228, 1533, 2063, 2359, 2606
TaiI ACGT 6 cut(s) 609, 1694, 2329, 2536, 2689, 2800
TaqI TCGA 5 cut(s) 123, 926, 1722, 1946, 2987
TaqII GACCGA 1 cut(s) 2085
TatI WGTACW 3 cut(s) 165, 1672, 2354
TscAI CASTG 8 cut(s) 333, 345, 1233, 1798, 1936, 2068, 2092, 2166
TseFI GTSAC 6 cut(s) 48, 324, 1091, 1505, 2597, 2951
TseI GCWGC 5 cut(s) 428, 1440, 2381, 2384, 2387
Tsp45I GTSAC 6 cut(s) 48, 324, 1091, 1505, 2597, 2951
TspGWI ACGGA 4 cut(s) 1079, 1382, 1891, 3166
TspRI CASTG 8 cut(s) 333, 345, 1233, 1798, 1936, 2068, 2092, 2166
Tth111I GACNNNGTC 1 cut(s) 1319
Van91I CCANNNNNTGG 1 cut(s) 2234
Vha464I CTTAAG 1 cut(s) 1157
VneI GTGCAC 1 cut(s) 272
VpaK11BI GGWCC 7 cut(s) 28, 505, 600, 1837, 2569, 3209, 3280
XagI CCTNNNNNAGG 3 cut(s) 594, 2421, 2903
XapI RAATTY 7 cut(s) 994, 1283, 1367, 1800, 1870, 2857, 2989
XceI RCATGY 1 cut(s) 3135
XmiI GTMKAC 3 cut(s) 215, 1396, 2671
XmnI GAANNNNTTC 6 cut(s) 539, 1085, 1166, 1860, 2304, 2736
XspI CTAG 1 cut(s) 1875
ZrmI AGTACT 1 cut(s) 2356
Zsp2I ATGCAT 2 cut(s) 1862, 3116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.