Rorug02G0230500

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0012264
Physical Location & Seq
Forward (+)
22982623 .. 22984505
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0230500.1

Sequence Viewer

Length: 1674 bp
ATGGTGATGCAGTGTGTAACTTCTGTCAGGTATTCGTTTTTGATTAAGGGTAAGCCAAGAGGGTTGTTTATCCCGAGTCGGGGGTTGAGACAAGGGGACCCTTTGTCACCCTATTTATTTCTGATTGGAGCGGAGGGCTTTTCGGCTTTATTACAACAGAAGCAGGAGATGGGGCTCTTGCCTGGGATTGAGGTATGTTCAGAGGCTCCTGCTATGAATCACCTGTTATTCGCAGATGATAGTATGATATATGCACAGGCTTCCTTGGAGGTGTGCTATGAGTTACAGGAGGTAATTGAGACGTATGGTCGAGCCTCTGGACAGCTAGTTAATTTTGATAAAAGTTCGGTGGTTTTTAGTAAGAATGTACCGAGTGTGATGCAGGGAGAGATATCGATTTTGTTGGGGGTGAAGGTAGTTGATTCTCATGAAAGATATCTTGGCCTTCCAACGTATGTGGGGAGGAAGAAGACTGCTACGTTCCAGTACATTAAGGAGAATTTGGCTAAAAAGATTTCTCATTGGCAGGGTAAATTTCTCAGTGGAGCCGAGAAGGATATTCTGATTAGGGTGGTTGCTCAAGCTCTACCCACTTATGCAATGAGTGTTTTTCAGTTAATAAATGGCTTCTGTGAAGATCTGGAGCAAATGTGCGCAAAGTTTTGGTGGGGAAGTACAACTGATAAAAGGAAAATTCATTGGAAGACTTGGAATGCTTTATGTAATCCTAAGAACGAAGGAGGGTTAGGGTTTCATAGCTTATCCACCTTTAACTCAGCCATGCTAGCTAAACAAGCTTGGCAGGTTATCTCTAATCCATCTTCTCTTGTGGCAAGGATTTATAAAGCGAAATATTATCCGGAGGATACATTTTGGTCAGCACCAGCACATGCTTCACCATCATATTCATGGCGAAGTATAGTCTCGACTAGAGAAATCTTGAAGGCTAACTCTTACTGGCAGATTGGAGATGGTTCCAATGTGCGAGTATTTTCGGACTGTTTGGTGCCGGAGTTACCATCTGGAAGGCCTAATGTAAGTTCTAGCTCAGTGGAGACGATACATATGGTTAGTGAGCTAATCACGGATACAGGATATTGGAACATTCCTTTACTACAGGACTTATTTTCTCCTGAGGAAGTCGAAGTTATTTCAAGGATCCCCTTAAGTCAACGGGTAGTTGAGGACAGATTGGTATGGAGGTTGGCTAAGGATGGGCGGTTTAAAGTCAAAACTACATATAGCAGCTTGGTGCAAGCTTCCTCGAGAATTGCCTCATTTGACCTTCCAGTGAATGATAGGTTTTGGCAAAAACTTTGGAAGGTGAACATCCAGAACTCAGCTAATTACATATGTGGAAGATGGATGCAGTTCTGTGTCCAATCCCTGTCGATGCAAAACTTGGGAATGTTTATTTGCTTGATGTGGGAAATTTGGAAGGAAAGGAATTGTCGTGTTTGGGAACAAAAGAGGAGTGCTGCTTGTGATGTTGTGTTGAAGGCCATGACTAGATTTCACGAGTTCAGCTTCCACAATTCAAAAGTGGTTAGCTTGAGTTCTCGAGGCTCTCGAAGGGGGCAGTGGTCTGCTCCACCTATAGGGATCCTCAAGGTGAATGTGGATGGAGCATTCCATCATACTACCCGGAAGGGTGGGGTCGGATTTGTAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012506 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017137 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0030258 GO:0030659 GO:0031090 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0035618 GO:0035619 GO:0040007 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044706 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048015 GO:0048017 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051704 GO:0051716 GO:0052742 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0097708 GO:0099402 GO:0120025 GO:0120038 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

557

Amino Acids

63.04

Weight (kDa)

9.24

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 15 - 128 5.8e-09 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09350 AT5G64070 AT5G64070
fragaria_vesca FvH4_1g24240
malus_domestica MD09G1261800.v1.1 MD17G1256600.v1.1
prunus_persica Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1
pyrus_communis pycom09g17610 pycom17g25880
rosa_chinensis RchiOBHm_Chr2g0120731
rosa_laevigata RLG00000018575
rosa_multiflora Rmu_sc0000319.1_g000011
rosa_roxburghii Rroxscaffold_2G00123200
rosa_rugosa Rorug02G0230500
rosa_samantha Rh2BG298500 Rh2CG276200
rosa_wichuraiana Rw2G023030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 843
Acc16I TGCGCA 1 cut(s) 655
Acc36I ACCTGC 1 cut(s) 793
AccB1I GGYRCC 1 cut(s) 1006
AccBSI CCGCTC 1 cut(s) 131
AccIII TCCGGA 1 cut(s) 859
AciI CCGC 2 cut(s) 131, 1219
AclWI GGATC 4 cut(s) 1153, 1166, 1597, 1610
AcsI RAATTY 4 cut(s) 499, 533, 693, 1431
AfaI GTAC 3 cut(s) 369, 488, 676
AfiI CCNNNNNNNGG 3 cut(s) 79, 80, 1598
AflII CTTAAG 1 cut(s) 1165
AgsI TTSAA 4 cut(s) 943, 1155, 1498, 1539
AhdI GACNNNNNGTC 2 cut(s) 103, 306
AjnI CCWGG 1 cut(s) 181
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
Alw26I GTCTC 4 cut(s) 82, 293, 928, 1049
AlwI GGATC 4 cut(s) 1153, 1166, 1597, 1610
Ama87I CYCGRG 3 cut(s) 73, 1264, 1560
Aor13HI TCCGGA 1 cut(s) 859
AoxI GGCC 3 cut(s) 442, 1028, 1500
ApeKI GCWGC 2 cut(s) 1245, 1478
ApoI RAATTY 4 cut(s) 499, 533, 693, 1431
AspLEI GCGC 1 cut(s) 656
AspS9I GGNCC 1 cut(s) 97
AsuC2I CCSGG 1 cut(s) 1645
AsuHPI GGTGA 7 cut(s) 16, 99, 212, 421, 888, 1336, 1624
AsuNHI GCTAGC 1 cut(s) 784
AvaI CYCGRG 3 cut(s) 73, 1264, 1560
AvaII GGWCC 1 cut(s) 97
AxyI CCTNAGG 1 cut(s) 1134
BamHI GGATCC 2 cut(s) 1158, 1602
BanI GGYRCC 1 cut(s) 1006
BanII GRGCYC 1 cut(s) 177
BauI CACGAG 1 cut(s) 1517
BbsI GAAGAC 2 cut(s) 476, 710
BbvI GCAGC 2 cut(s) 1257, 1465
BccI CCATC 9 cut(s) 163, 826, 907, 965, 1027, 1208, 1356, 1616, 1641
BcgI CGANNNNNNTGC 2 cut(s) 361, 395
BciT130I CCWGG 1 cut(s) 183
BciVI GTATCC 2 cut(s) 859, 1081
BcnI CCSGG 1 cut(s) 1645
BcoDI GTCTC 4 cut(s) 82, 293, 928, 1049
BfaI CTAG 5 cut(s) 326, 785, 930, 1044, 1509
BfmI CTRYAG 2 cut(s) 1115, 1596
BfrI CTTAAG 1 cut(s) 1165
BfuAI ACCTGC 1 cut(s) 793
BfuI GTATCC 2 cut(s) 859, 1081
BglII AGATCT 1 cut(s) 637
BisI GCNGC 2 cut(s) 1246, 1479
BlsI GCNGC 2 cut(s) 1247, 1480
Bme1390I CCNGG 2 cut(s) 183, 1645
Bme18I GGWCC 1 cut(s) 97
BmeRI GACNNNNNGTC 2 cut(s) 103, 306
BmeT110I CYCGRG 3 cut(s) 73, 1264, 1560
BmgT120I GGNCC 1 cut(s) 97
BmiI GGNNCC 8 cut(s) 98, 99, 207, 547, 976, 1008, 1160, 1604
BmrFI CCNGG 2 cut(s) 183, 1645
BmsI GCATC 3 cut(s) 369, 1356, 1383
BmtI GCTAGC 1 cut(s) 788
BpiI GAAGAC 2 cut(s) 476, 710
BpmI CTGGAG 1 cut(s) 662
Bpu10I CCTNAGC 1 cut(s) 1209
BpuEI CTTGAG 3 cut(s) 564, 1573, 1592
BpuMI CCSGG 1 cut(s) 1645
Bsa29I ATCGAT 1 cut(s) 395
BsaJI CCNNGG 2 cut(s) 182, 264
BsaWI WCCGGW 1 cut(s) 859
BsaXI ACNNNNNCTCC 2 cut(s) 635, 665
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 80, 1598
Bse1I ACTGG 3 cut(s) 484, 962, 1289
Bse21I CCTNAGG 1 cut(s) 1134
Bse3DI GCAATG 1 cut(s) 606
BseAI TCCGGA 1 cut(s) 859
BseBI CCWGG 1 cut(s) 183
BseCI ATCGAT 1 cut(s) 395
BseDI CCNNGG 2 cut(s) 182, 264
BseGI GGATG 4 cut(s) 1219, 1329, 1371, 1627
BseLI CCNNNNNNNGG 3 cut(s) 79, 80, 1598
BseMI GCAATG 1 cut(s) 606
BseMII CTCAG 5 cut(s) 553, 789, 1062, 1125, 1353
BseNI ACTGG 3 cut(s) 484, 962, 1289
BseRI GAGGAG 1 cut(s) 1486
BseXI GCAGC 2 cut(s) 1257, 1465
BshFI GGCC 3 cut(s) 444, 1030, 1502
BshNI GGYRCC 1 cut(s) 1006
BshVI ATCGAT 1 cut(s) 395
BsiHKCI CYCGRG 3 cut(s) 73, 1264, 1560
BsiSI CCGG 3 cut(s) 860, 1010, 1645
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 3 cut(s) 79, 80, 1598
BsmAI GTCTC 4 cut(s) 82, 293, 928, 1049
BsmBI CGTCTC 2 cut(s) 293, 1049
BsmFI GGGAC 1 cut(s) 110
BsmI GAATGC 2 cut(s) 718, 1628
BsnI GGCC 3 cut(s) 444, 1030, 1502
BsoBI CYCGRG 3 cut(s) 73, 1264, 1560
Bsp1286I GDGCHC 1 cut(s) 177
Bsp13I TCCGGA 1 cut(s) 859
Bsp143I GATC 3 cut(s) 637, 1158, 1602
BspACI CCGC 2 cut(s) 131, 1219
BspANI GGCC 3 cut(s) 444, 1030, 1502
BspCNI CTCAG 5 cut(s) 552, 788, 1061, 1126, 1352
BspDI ATCGAT 1 cut(s) 395
BspEI TCCGGA 1 cut(s) 859
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 8 cut(s) 98, 99, 207, 547, 976, 1008, 1160, 1604
BspMI ACCTGC 1 cut(s) 793
BspOI GCTAGC 1 cut(s) 788
BspPI GGATC 4 cut(s) 1153, 1166, 1597, 1610
BspT107I GGYRCC 1 cut(s) 1006
BspTI CTTAAG 1 cut(s) 1165
BsrBI CCGCTC 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 606
BsrI ACTGG 3 cut(s) 484, 962, 1289
BssECI CCNNGG 2 cut(s) 182, 264
BssMI GATC 3 cut(s) 637, 1158, 1602
BssSI CACGAG 1 cut(s) 1517
BssT1I CCWWGG 1 cut(s) 264
Bst2BI CACGAG 1 cut(s) 1517
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 1001
BstAFI CTTAAG 1 cut(s) 1165
BstC8I GCNNGC 2 cut(s) 786, 1257
BstDEI CTNAG 7 cut(s) 539, 729, 775, 1048, 1134, 1209, 1339
BstF5I GGATG 4 cut(s) 1219, 1329, 1371, 1627
BstHHI GCGC 1 cut(s) 656
BstKTI GATC 3 cut(s) 640, 1161, 1605
BstMAI GTCTC 4 cut(s) 82, 293, 928, 1049
BstMBI GATC 3 cut(s) 637, 1158, 1602
BstMWI GCNNNNNNNGC 2 cut(s) 785, 794
BstNI CCWGG 1 cut(s) 183
BstNSI RCATGY 1 cut(s) 893
BstSCI CCNGG 2 cut(s) 181, 1643
BstSFI CTRYAG 2 cut(s) 1115, 1596
BstV1I GCAGC 2 cut(s) 1257, 1465
BstV2I GAAGAC 2 cut(s) 476, 710
BstX2I RGATCY 3 cut(s) 637, 1158, 1602
BstYI RGATCY 3 cut(s) 637, 1158, 1602
Bsu15I ATCGAT 1 cut(s) 395
Bsu36I CCTNAGG 1 cut(s) 1134
BsuI GTATCC 2 cut(s) 859, 1081
BsuRI GGCC 3 cut(s) 444, 1030, 1502
BsuTUI ATCGAT 1 cut(s) 395
BtsCI GGATG 4 cut(s) 1219, 1329, 1371, 1627
BtsI GCAGTG 2 cut(s) 17, 1586
BtsIMutI CAGTG 5 cut(s) 17, 547, 1056, 1296, 1586
BveI ACCTGC 1 cut(s) 793
Cac8I GCNNGC 2 cut(s) 786, 1257
CciI TCATGA 1 cut(s) 427
CfoI GCGC 1 cut(s) 656
Cfr13I GGNCC 1 cut(s) 97
ClaI ATCGAT 1 cut(s) 395
Csp6I GTAC 3 cut(s) 368, 487, 675
CspCI CAANNNNNGTGG 2 cut(s) 438, 473
CviAII CATG 5 cut(s) 428, 781, 890, 909, 1504
CviQI GTAC 3 cut(s) 368, 487, 675
DdeI CTNAG 7 cut(s) 539, 729, 775, 1048, 1134, 1209, 1339
DpnI GATC 3 cut(s) 639, 1160, 1604
DpnII GATC 3 cut(s) 637, 1158, 1602
DraI TTTAAA 1 cut(s) 1225
DriI GACNNNNNGTC 2 cut(s) 103, 306
Eam1105I GACNNNNNGTC 2 cut(s) 103, 306
Eco130I CCWWGG 1 cut(s) 264
Eco147I AGGCCT 1 cut(s) 1030
Eco24I GRGCYC 1 cut(s) 177
Eco32I GATATC 2 cut(s) 393, 437
Eco47I GGWCC 1 cut(s) 97
Eco81I CCTNAGG 1 cut(s) 1134
Eco88I CYCGRG 3 cut(s) 73, 1264, 1560
EcoO109I RGGNCCY 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 181
EcoRV GATATC 2 cut(s) 393, 437
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 264
Esp3I CGTCTC 2 cut(s) 293, 1049
FaeI CATG 5 cut(s) 431, 784, 893, 912, 1507
FaqI GGGAC 1 cut(s) 110
FatI CATG 5 cut(s) 427, 780, 889, 908, 1503
FauNDI CATATG 2 cut(s) 1065, 1352
Fnu4HI GCNGC 2 cut(s) 1246, 1479
FokI GGATG 4 cut(s) 1226, 1316, 1378, 1634
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 2 cut(s) 1246, 1479
FspBI CTAG 5 cut(s) 326, 785, 930, 1044, 1509
FspI TGCGCA 1 cut(s) 655
GlaI GCGC 1 cut(s) 655
GluI GCNGC 2 cut(s) 1246, 1479
GsuI CTGGAG 1 cut(s) 662
HaeIII GGCC 3 cut(s) 444, 1030, 1502
HapII CCGG 3 cut(s) 860, 1010, 1645
HhaI GCGC 1 cut(s) 656
Hin1II CATG 5 cut(s) 431, 784, 893, 912, 1507
Hin6I GCGC 1 cut(s) 654
HinP1I GCGC 1 cut(s) 654
HincII GTYRAC 1 cut(s) 1172
HindII GTYRAC 1 cut(s) 1172
HindIII AAGCTT 2 cut(s) 795, 1257
HinfI GANTC 3 cut(s) 76, 217, 422
HpaII CCGG 3 cut(s) 860, 1010, 1645
HphI GGTGA 7 cut(s) 16, 99, 212, 421, 888, 1336, 1624
Hpy166II GTNNAC 2 cut(s) 1172, 1327
Hpy188I TCNGA 6 cut(s) 123, 202, 564, 997, 1661, 1673
Hpy8I GTNNAC 2 cut(s) 1172, 1327
HpyCH4III ACNGT 1 cut(s) 1001
HpyCH4IV ACGT 3 cut(s) 302, 452, 479
HpyCH4V TGCA 7 cut(s) 10, 254, 382, 599, 1255, 1369, 1396
HpyF10VI GCNNNNNNNGC 2 cut(s) 785, 794
HpyF3I CTNAG 7 cut(s) 539, 729, 775, 1048, 1134, 1209, 1339
HpySE526I ACGT 3 cut(s) 302, 452, 479
Hsp92II CATG 5 cut(s) 431, 784, 893, 912, 1507
HspAI GCGC 1 cut(s) 654
KflI GGGWCCC 1 cut(s) 97
Kpn2I TCCGGA 1 cut(s) 859
Kzo9I GATC 3 cut(s) 637, 1158, 1602
LmnI GCTCC 6 cut(s) 128, 211, 545, 643, 1594, 1625
Lsp1109I GCAGC 2 cut(s) 1257, 1465
LweI GCATC 3 cut(s) 369, 1356, 1383
MaeI CTAG 5 cut(s) 326, 785, 930, 1044, 1509
MaeII ACGT 3 cut(s) 302, 452, 479
MaeIII GTNAC 4 cut(s) 16, 105, 282, 1014
MalI GATC 3 cut(s) 639, 1160, 1604
MbiI CCGCTC 1 cut(s) 131
MboI GATC 3 cut(s) 637, 1158, 1602
MboII GAAGA 6 cut(s) 478, 481, 647, 715, 813, 1371
MflI RGATCY 3 cut(s) 637, 1158, 1602
MhlI GDGCHC 1 cut(s) 177
MlyI GAGTC 1 cut(s) 85
MmeI TCCRAC 2 cut(s) 473, 1639
MroI TCCGGA 1 cut(s) 859
MseI TTAA 7 cut(s) 45, 330, 492, 617, 771, 1166, 1224
MslI CAYNNNNRTG 1 cut(s) 907
MspCI CTTAAG 1 cut(s) 1165
MspI CCGG 3 cut(s) 860, 1010, 1645
MspR9I CCNGG 2 cut(s) 183, 1645
Mva1269I GAATGC 2 cut(s) 718, 1628
MvaI CCWGG 1 cut(s) 183
MwoI GCNNNNNNNGC 2 cut(s) 785, 794
NciI CCSGG 1 cut(s) 1645
NdeI CATATG 2 cut(s) 1065, 1352
NdeII GATC 3 cut(s) 637, 1158, 1602
NheI GCTAGC 1 cut(s) 784
NlaIII CATG 5 cut(s) 431, 784, 893, 912, 1507
NlaIV GGNNCC 8 cut(s) 98, 99, 207, 547, 976, 1008, 1160, 1604
NmeAIII GCCGAG 1 cut(s) 574
NmuCI GTSAC 1 cut(s) 105
NsbI TGCGCA 1 cut(s) 655
NspI RCATGY 1 cut(s) 893
PaeR7I CTCGAG 2 cut(s) 1264, 1560
PagI TCATGA 1 cut(s) 427
PceI AGGCCT 1 cut(s) 1030
PcsI WCGNNNNNNNCGW 1 cut(s) 1567
PctI GAATGC 2 cut(s) 718, 1628
PfeI GAWTC 2 cut(s) 217, 422
PkrI GCNGC 2 cut(s) 1247, 1480
PleI GAGTC 1 cut(s) 84
PpsI GAGTC 1 cut(s) 84
PpuMI RGGWCCY 1 cut(s) 97
PsiI TTATAA 1 cut(s) 843
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 8 cut(s) 98, 99, 207, 547, 976, 1008, 1160, 1604
PspPI GGNCC 1 cut(s) 97
PspPPI RGGWCCY 1 cut(s) 97
PsuI RGATCY 3 cut(s) 637, 1158, 1602
RsaI GTAC 3 cut(s) 369, 488, 676
RsaNI GTAC 3 cut(s) 368, 487, 675
RseI CAYNNNNRTG 1 cut(s) 907
SaqAI TTAA 7 cut(s) 45, 330, 492, 617, 771, 1166, 1224
SatI GCNGC 2 cut(s) 1246, 1479
Sau3AI GATC 3 cut(s) 637, 1158, 1602
Sau96I GGNCC 1 cut(s) 97
SchI GAGTC 1 cut(s) 85
ScrFI CCNGG 2 cut(s) 183, 1645
SduI GDGCHC 1 cut(s) 177
SfaNI GCATC 3 cut(s) 369, 1356, 1383
SfcI CTRYAG 2 cut(s) 1115, 1596
Sfr274I CTCGAG 2 cut(s) 1264, 1560
SinI GGWCC 1 cut(s) 97
SlaI CTCGAG 2 cut(s) 1264, 1560
SmiMI CAYNNNNRTG 1 cut(s) 907
SmlI CTYRAG 6 cut(s) 579, 1165, 1264, 1552, 1560, 1607
SmoI CTYRAG 6 cut(s) 579, 1165, 1264, 1552, 1560, 1607
SseBI AGGCCT 1 cut(s) 1030
SsiI CCGC 2 cut(s) 131, 1219
SspI AATATT 1 cut(s) 854
SspMI CTAG 5 cut(s) 326, 785, 930, 1044, 1509
StuI AGGCCT 1 cut(s) 1030
StyD4I CCNGG 2 cut(s) 181, 1643
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 1 cut(s) 1001
TaiI ACGT 3 cut(s) 305, 455, 482
TaqI TCGA 8 cut(s) 310, 395, 926, 1143, 1265, 1391, 1561, 1570
TatI WGTACW 2 cut(s) 486, 674
TfiI GAWTC 2 cut(s) 217, 422
Tru1I TTAA 7 cut(s) 45, 330, 492, 617, 771, 1166, 1224
Tru9I TTAA 7 cut(s) 45, 330, 492, 617, 771, 1166, 1224
TscAI CASTG 5 cut(s) 17, 547, 1056, 1296, 1586
TseFI GTSAC 1 cut(s) 105
TseI GCWGC 2 cut(s) 1245, 1478
Tsp45I GTSAC 1 cut(s) 105
TspDTI ATGAA 5 cut(s) 230, 444, 686, 743, 897
TspGWI ACGGA 1 cut(s) 1100
TspRI CASTG 5 cut(s) 17, 547, 1056, 1296, 1586
Vha464I CTTAAG 1 cut(s) 1165
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 4 cut(s) 499, 533, 693, 1431
XceI RCATGY 1 cut(s) 893
XcmI CCANNNNNNNNNTGG 1 cut(s) 906
XhoI CTCGAG 2 cut(s) 1264, 1560
XspI CTAG 5 cut(s) 326, 785, 930, 1044, 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.