MD17G1256600.v1.1

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012264
Physical Location & Seq
Reverse (-)
31707406 .. 31732165
24760 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1256600.v1.1.491

Sequence Viewer

Length: 3261 bp
ATGGATGGCACCATTTTAAAATTCCGCCTTGGGTCCTCAAAAGTTCAGGGAGAGTCGCCCCGAGAGATCACCTCTCGGACGAACCTGTCGAGTGACACCAGTGAGAATGGGTGGCTCATACGGTTCTTTGACTCAGCATTTTTCTGTGAGTGGATAGCTGTTAGCTACCTGTACAAGCATGACCATGCAGGGGTGCGCGACTACCTCTGTAATCGAATGTACACTCTTCCCTTATCGGGAATTGAGAGCTACTTGTTTCAGATATGTTACATGTCAGTGCACAAGCCAAGCCCGTCTTTGGATAAGTTTGTTATTGATATGTGCTCTAAGTCGCTTAAGATAGCTCAGAAGGTGCATTGGTTTTTATTGGCGGAGCTTGAAGACTCAGATGATAATGAAGGAATTAGTAGAATTCAGGAGAAGTGTCAAATTGCGGCCACTTTGATGGGTGAGTGGCCGCCTTTGGTACGTCCTCAAAATGAGTCAGCTAGCCCTGGGAGCAAGAACCAGGTATTGAATAGAATATTGTCATCAAAACAGAAATTGTTGTCACTAACGTCTTCACCACCTGCCCAGAGGTCATTTTCTTTCTCGCCTTCATCGGTAAACAATTTGCAAGAGGATGGTGGTCAGTTCTCTCCCGATGAGAACAAAATTTTTAAAAAATTCATACCAGGGCCGAAGGTTCGGGATGCATTGCTCTTTAGGAAGTCAATGGAGAAAGGTGATGATGACTCTGAAAAAGATGGATTCTTTAAGAGGCTTTTAAGAGACAGTAGAGGTGATGATGAGACAGGTTCTAAAATTAGAGATTCATTGCTTTTTAGAAAGTCCTCAGAGAAGGATGATGATGATGCAGAGAAGGTTGGCTTCTTTAAGAGGCTATTAAGGGATAGTAAAGGCGACGATGAGGAATTGACATCGAGCTCAGAGGGTTTTTTTAAGAGATTGTTTCGTGATAGTAAAAGTGATTCTGACGATAAATCAATCTCTAAATCAGTGAAAGATGAAGAAAAAGAAGGGTTCTTTCGGAAGTTTTTCAAAGAGAAGTTTGAGGACAAAAAGGACCGGATTGATAAGAATGAAGATGATGATGCACCATACTCTGAAGAAAAAGATTTGAAATCTGCTGAAGATGATGAAAAAGAGGGGTTCTTCCGAAAAATTTTCAGAGATAAGTTTGATGACAAGAAAGATGGGAATGATAAAATCGAGGAGGGGAGTGCCAATGGAGACGAAGAAGAGCCTTCTGACTTCCCATTGTTTCGAAGATTGTTCCGTGTGCACCCTGAAGATGCAAAAAGTACCGTTGCAAATGAAAACAGCAGTACCGGGGGCTTGCTAGAAAGTAGTCCGGGGACTGAGAACTTTTTTCGCAAATTGTTTAGAGACCGTGACCGTTCAGTTGAAGACTCAGAGCTATTTGGTTCAAGAAAACACAAAGAGAAACGCCCTGGTTCTCCAAAGCAACAAAATGAAAAGTCAAGTGCTAAACCCCCACTTCCAAATGCTACAGCATCCCAATACCGTAAAGGGGCTTATCATGAGTCGCTGGACTTTGTGCAGTCACTATGTGAGACATCTTATGGCTTAGTGGATATATTTCCAATTGAGGATCGCAAGAGTGCTCTTCGTGAGTCACTTGCAGAGATCAATTTGCACATTGCTGAGGCTCAAAGTAGTGGAGGAGTTTGCTTTCCAATGGGTAAGGGGATGTATCGCGTGGTTTATATACCGGAAGATGAAGCTGTTCTTTTGAATTCTAGGGAAAAGGCACCGTATCTCATCTGTGTTGAAGTCCTGAAAAGTGAAATTCCTGGTGCAAAGGATGCATCTGGTTCTCAAAGGCTTTCTCGAGGAGGAATTCCTTTAGCAAATGGGGATGCACTTCTGCCAAGACCGCCTCCGTGGGCATATCCTTTGTGGACTGTTCAGGAGGTCTATCGCAATAGTAATGATAGGATGTCGAGTTCTACAGCTCAAGCAATTGACCAGGCGATGTCATATACGTCCGAGGCGAAAGTGAAATTTGTTACTGTAAAAATATCTGTGGAGAATAAATTGCATAGTCATACAGTAAAAGCAGAGAATATATCAAGGAGTTATCAACGTGGAGAGGCTGCGACTTGTGCACAAGGGAGTGATTTGGAGTGGGTAAGGGTGGTGCTTACAGCAGACCCTGGTGTTAGAATGGAGGACATTGAGGATCAAGGACCCCCTCGGCGGAAGGAACACCGTCGTGTACCAAGTACAGTGGCTATAGAGGAAGTAAAGGCTGCTGCAGCGAAAGGAGAAGCACCTCCTGGACTTCCTCTGAAAGGGGCTGGTCAGGATTCATCAGATGCACAGCCAATGGCTAATGGTGGTATTCCCAAGGCCAGCAATGCCTTGTCTGGCGAACTTTGGGAGGTAAAGAAGGAGAGAATAAGAAAAGCTTCAGTTCATGGAAAGTTACCTGGTTGGGACTTGTGCTCCGTTATTGTGAAGAGTGGTGATGATTGTAGGCAGGAGCATCTTGCTGTGCAACTTATTTCTCACTTTTATGATATATTTCAAGAAGCTGGTCTCCCTCTCTGGTTGCGCCCTTATGAAGTCTTAGTTACTTCTTCTTATACAGCTCTCATTGAAACAATTCCAGATACGGCTTCAATTCATTCTATCAAAAGTAGATATCCCAACATCACGAGCTTGCGAGACTTCTTTGCTGCTAAGTACCAAGAAAATTCTCCTAGTTTTAAACTTGCTCAGAGGAATTTTGTTGAAAGTATGGCTGGATATTCTCTAGTGTGTTACCTTCTGCAGATAAAGGATAGGCACAATGGGAACCTCTTAATGGATGAAGAAGGTCATATTATACATATTGATTTTGGTTTCATGCTCTCCAATTCGCCTGGGGGTGTAAATTTTGAGAGTGCTCCATTCAAATTAACACGTGAACTTCTTGAGGTCATGGACTCTGATGCTGAGGGAGTTCCTAGCGAGTTCTTTGATTATTTTAAGGTTTTGTGCATTCAAGGATTCCTTACATGTCGGAAGCATGCAGAACGCATTATTCTTCTTGTTGAGATGTTGCAGGACTCTGGTTTCCCATGCTTTAAAGGTGGTACACGAACAATACAGAACCTACGGAAAAGATTTCACTTGAATTTGACAGAAGAGCAATGCGTATCCTTGGTGCTTTCGTTGATCAGCAGCAGCTTGGACGCGTGGCGAACAAGGCAATATGATTATTATCAGAGGGTTTTGAATGGAATATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012506 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017137 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0030258 GO:0030659 GO:0031090 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0035618 GO:0035619 GO:0040007 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044706 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048015 GO:0048017 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051704 GO:0051716 GO:0052742 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0097708 GO:0099402 GO:0120025 GO:0120038 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

1087

Amino Acids

122.57

Weight (kDa)

5.81

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI4KB-PIK1_PIK PF21245 52 - 122 4.7e-11 PI4KB/PIK1, accessory (PIK) domain
PI3_PI4_kinase PF00454 825 - 1033 8e-39 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09350 AT5G64070 AT5G64070
fragaria_vesca FvH4_1g24240
malus_domestica MD09G1261800.v1.1 MD17G1256600.v1.1
prunus_persica Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1
pyrus_communis pycom09g17610 pycom17g25880
rosa_chinensis RchiOBHm_Chr2g0120731
rosa_laevigata RLG00000018575
rosa_multiflora Rmu_sc0000319.1_g000011
rosa_roxburghii Rroxscaffold_2G00123200
rosa_rugosa Rorug02G0230500
rosa_samantha Rh2BG298500 Rh2CG276200
rosa_wichuraiana Rw2G023030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 577
AasI GACNNNNNNGTC 1 cut(s) 85
Acc36I ACCTGC 1 cut(s) 577
AccB1I GGYRCC 2 cut(s) 8, 1774
AccII CGCG 3 cut(s) 198, 1722, 3206
AciI CCGC 6 cut(s) 25, 371, 434, 458, 1901, 2224
AclWI GGATC 2 cut(s) 1623, 2214
AcoI YGGCCR 2 cut(s) 435, 455
AcuI CTGAAG 4 cut(s) 1128, 1152, 1311, 2421
AcvI CACGTG 1 cut(s) 2933
AdeI CACNNNGTG 1 cut(s) 1574
AfaI GTAC 9 cut(s) 173, 221, 468, 1306, 1330, 2244, 2251, 2714, 3106
AfiI CCNNNNNNNGG 7 cut(s) 190, 236, 298, 1534, 2223, 2318, 2833
AflII CTTAAG 1 cut(s) 335
AflIII ACRYGT 4 cut(s) 270, 2930, 3026, 3204
AleI CACNNNNGTG 1 cut(s) 2238
AloI GAACNNNNNNTCC 2 cut(s) 1954, 1986
Alw21I GWGCWC 8 cut(s) 282, 326, 929, 1287, 1630, 2134, 2474, 2917
Alw26I GTCTC 7 cut(s) 765, 785, 1227, 1383, 1571, 2570, 2688
Alw44I GTGCAC 3 cut(s) 278, 1283, 2130
AlwI GGATC 2 cut(s) 1623, 2214
AlwNI CAGNNNCTG 1 cut(s) 2180
Ama87I CYCGRG 2 cut(s) 60, 1854
AoxI GGCC 4 cut(s) 435, 455, 677, 2376
ApaLI GTGCAC 3 cut(s) 278, 1283, 2130
ApeKI GCWGC 7 cut(s) 2120, 2276, 2279, 2282, 2705, 3192, 3195
Asp700I GAANNNNTTC 4 cut(s) 1037, 1749, 2434, 2631
AspLEI GCGC 2 cut(s) 198, 2583
AspS9I GGNCC 4 cut(s) 33, 677, 1066, 2213
AsuC2I CCSGG 2 cut(s) 1333, 1356
AsuHPI GGTGA 6 cut(s) 61, 461, 555, 737, 794, 2504
AsuII TTCGAA 1 cut(s) 1267
AsuNHI GCTAGC 1 cut(s) 488
AvaI CYCGRG 2 cut(s) 60, 1854
AvaII GGWCC 3 cut(s) 33, 1066, 2213
BaeGI GKGCMC 3 cut(s) 282, 1287, 2134
BaeI ACNNNNGTAYC 2 cut(s) 1312, 1345
BanI GGYRCC 2 cut(s) 8, 1774
BanII GRGCYC 1 cut(s) 929
BauI CACGAG 1 cut(s) 2683
BbrPI CACGTG 1 cut(s) 2933
BbsI GAAGAC 3 cut(s) 387, 552, 1416
Bbv12I GWGCWC 8 cut(s) 282, 326, 929, 1287, 1630, 2134, 2474, 2917
BbvCI CCTCAGC 2 cut(s) 1668, 2964
BbvI GCAGC 7 cut(s) 2107, 2263, 2266, 2294, 2692, 3204, 3207
BccI CCATC 4 cut(s) 439, 617, 740, 1190
BceAI ACGGC 1 cut(s) 2658
BciVI GTATCC 1 cut(s) 3178
BclI TGATCA 1 cut(s) 3186
BcnI CCSGG 2 cut(s) 1333, 1356
BcoDI GTCTC 7 cut(s) 765, 785, 1227, 1383, 1571, 2570, 2688
BfaI CTAG 6 cut(s) 489, 1343, 1764, 2730, 2783, 2976
BfmI CTRYAG 5 cut(s) 1512, 1974, 2259, 2280, 2798
BfrI CTTAAG 1 cut(s) 335
BfuAI ACCTGC 1 cut(s) 577
BfuI GTATCC 1 cut(s) 3178
BisI GCNGC 9 cut(s) 435, 458, 2121, 2277, 2280, 2283, 2706, 3193, 3196
BlsI GCNGC 9 cut(s) 436, 459, 2122, 2278, 2281, 2284, 2707, 3194, 3197
Bme18I GGWCC 3 cut(s) 33, 1066, 2213
BmeT110I CYCGRG 2 cut(s) 60, 1854
BmgT120I GGNCC 4 cut(s) 33, 677, 1066, 2213
BmiI GGNNCC 5 cut(s) 10, 34, 1776, 2215, 2825
BmtI GCTAGC 1 cut(s) 492
BpiI GAAGAC 3 cut(s) 387, 552, 1416
BplI GAGNNNNNCTC 2 cut(s) 56, 88
Bpu10I CCTNAGC 2 cut(s) 1668, 2964
Bpu14I TTCGAA 1 cut(s) 1267
BpuEI CTTGAG 2 cut(s) 1965, 2963
BpuMI CCSGG 2 cut(s) 1333, 1356
BsaAI YACGTR 1 cut(s) 2933
BsaBI GATNNNNATC 1 cut(s) 3231
BsaI GGTCTC 2 cut(s) 1383, 2570
BsaWI WCCGGW 2 cut(s) 1068, 1735
BsaXI ACNNNNNCTCC 2 cut(s) 2456, 2486
Bsc4I CCNNNNNNNGG 7 cut(s) 190, 236, 298, 1534, 2223, 2318, 2833
Bse1I ACTGG 1 cut(s) 99
Bse3DI GCAATG 5 cut(s) 695, 815, 1662, 2389, 3167
Bse8I GATNNNNATC 1 cut(s) 3231
BseJI GATNNNNATC 1 cut(s) 3231
BseLI CCNNNNNNNGG 7 cut(s) 190, 236, 298, 1534, 2223, 2318, 2833
BseMI GCAATG 5 cut(s) 695, 815, 1662, 2389, 3167
BseNI ACTGG 1 cut(s) 99
BseRI GAGGAG 3 cut(s) 1229, 1701, 1872
BseSI GKGCMC 3 cut(s) 282, 1287, 2134
BseXI GCAGC 7 cut(s) 2107, 2263, 2266, 2294, 2692, 3204, 3207
BsgI GTGCAG 1 cut(s) 1583
Bsh1236I CGCG 3 cut(s) 198, 1722, 3206
BshFI GGCC 4 cut(s) 437, 457, 679, 2378
BshNI GGYRCC 2 cut(s) 8, 1774
BsiHKAI GWGCWC 8 cut(s) 282, 326, 929, 1287, 1630, 2134, 2474, 2917
BsiHKCI CYCGRG 2 cut(s) 60, 1854
BsiSI CCGG 4 cut(s) 1069, 1332, 1355, 1736
BslFI GGGAC 2 cut(s) 1372, 2477
BslI CCNNNNNNNGG 7 cut(s) 190, 236, 298, 1534, 2223, 2318, 2833
BsmAI GTCTC 7 cut(s) 765, 785, 1227, 1383, 1571, 2570, 2688
BsmBI CGTCTC 1 cut(s) 1227
BsmFI GGGAC 2 cut(s) 1372, 2477
BsmI GAATGC 1 cut(s) 3009
BsnI GGCC 4 cut(s) 437, 457, 679, 2378
Bso31I GGTCTC 2 cut(s) 1383, 2570
BsoBI CYCGRG 2 cut(s) 60, 1854
Bsp119I TTCGAA 1 cut(s) 1267
Bsp1286I GDGCHC 8 cut(s) 282, 326, 929, 1287, 1630, 2134, 2474, 2917
Bsp1407I TGTACA 2 cut(s) 171, 219
Bsp143I GATC 5 cut(s) 66, 1615, 1650, 2206, 3186
BspACI CCGC 6 cut(s) 25, 371, 434, 458, 1901, 2224
BspANI GGCC 4 cut(s) 437, 457, 679, 2378
BspFNI CGCG 3 cut(s) 198, 1722, 3206
BspHI TCATGA 1 cut(s) 1543
BspLI GGNNCC 5 cut(s) 10, 34, 1776, 2215, 2825
BspMAI CTGCAG 2 cut(s) 2284, 2802
BspMI ACCTGC 1 cut(s) 577
BspOI GCTAGC 1 cut(s) 492
BspPI GGATC 2 cut(s) 1623, 2214
BspQI GCTCTTC 3 cut(s) 1236, 1635, 3150
BspT104I TTCGAA 1 cut(s) 1267
BspT107I GGYRCC 2 cut(s) 8, 1774
BspTI CTTAAG 1 cut(s) 335
BspTNI GGTCTC 2 cut(s) 1383, 2570
BsrDI GCAATG 5 cut(s) 695, 815, 1662, 2389, 3167
BsrGI TGTACA 2 cut(s) 171, 219
BsrI ACTGG 1 cut(s) 99
BssMI GATC 5 cut(s) 66, 1615, 1650, 2206, 3186
BssSI CACGAG 1 cut(s) 2683
BssT1I CCWWGG 3 cut(s) 28, 2373, 3171
Bst2BI CACGAG 1 cut(s) 2683
Bst6I CTCTTC 5 cut(s) 231, 1236, 1635, 2480, 3150
BstAFI CTTAAG 1 cut(s) 335
BstAPI GCANNNNNTGC 1 cut(s) 1829
BstAUI TGTACA 2 cut(s) 171, 219
BstBAI YACGTR 1 cut(s) 2933
BstBI TTCGAA 1 cut(s) 1267
BstC8I GCNNGC 5 cut(s) 490, 1340, 2380, 2690, 3039
BstDSI CCRYGG 1 cut(s) 1907
BstENI CCTNNNNNAGG 1 cut(s) 2316
BstFNI CGCG 3 cut(s) 198, 1722, 3206
BstHHI GCGC 2 cut(s) 198, 2583
BstKTI GATC 5 cut(s) 69, 1618, 1653, 2209, 3189
BstMAI GTCTC 7 cut(s) 765, 785, 1227, 1383, 1571, 2570, 2688
BstMBI GATC 5 cut(s) 66, 1615, 1650, 2206, 3186
BstMWI GCNNNNNNNGC 7 cut(s) 498, 1829, 1900, 2129, 2282, 2384, 3217
BstNSI RCATGY 3 cut(s) 274, 3030, 3041
BstSFI CTRYAG 5 cut(s) 1512, 1974, 2259, 2280, 2798
BstSLI GKGCMC 3 cut(s) 282, 1287, 2134
BstUI CGCG 3 cut(s) 198, 1722, 3206
BstV1I GCAGC 7 cut(s) 2107, 2263, 2266, 2294, 2692, 3204, 3207
BstV2I GAAGAC 3 cut(s) 387, 552, 1416
BstXI CCANNNNNNTGG 1 cut(s) 445
BsuI GTATCC 1 cut(s) 3178
BsuRI GGCC 4 cut(s) 437, 457, 679, 2378
BtgI CCRYGG 1 cut(s) 1907
BtgZI GCGATG 1 cut(s) 2012
BtsIMutI CAGTG 4 cut(s) 106, 282, 1005, 2259
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 5 cut(s) 490, 1340, 2380, 2690, 3039
CaiI CAGNNNCTG 1 cut(s) 2180
CciI TCATGA 1 cut(s) 1543
CfoI GCGC 2 cut(s) 198, 2583
Cfr13I GGNCC 4 cut(s) 33, 677, 1066, 2213
CseI GACGC 1 cut(s) 3212
CsiI ACCWGGT 2 cut(s) 507, 2455
Csp6I GTAC 9 cut(s) 172, 220, 467, 1305, 1329, 2243, 2250, 2713, 3105
CspCI CAANNNNNGTGG 2 cut(s) 2235, 2270
CviQI GTAC 9 cut(s) 172, 220, 467, 1305, 1329, 2243, 2250, 2713, 3105
DpnI GATC 5 cut(s) 68, 1617, 1652, 2208, 3188
DpnII GATC 5 cut(s) 66, 1615, 1650, 2206, 3186
DraI TTTAAA 4 cut(s) 18, 661, 2737, 3097
DraIII CACNNNGTG 1 cut(s) 1574
DrdI GACNNNNNNGTC 1 cut(s) 85
DseDI GACNNNNNNGTC 1 cut(s) 85
EaeI YGGCCR 2 cut(s) 435, 455
Eam1104I CTCTTC 5 cut(s) 231, 1236, 1635, 2480, 3150
EarI CTCTTC 5 cut(s) 231, 1236, 1635, 2480, 3150
EciI GGCGGA 3 cut(s) 14, 386, 2239
Ecl136II GAGCTC 1 cut(s) 927
Eco130I CCWWGG 3 cut(s) 28, 2373, 3171
Eco24I GRGCYC 1 cut(s) 929
Eco31I GGTCTC 2 cut(s) 1383, 2570
Eco32I GATATC 1 cut(s) 2672
Eco47I GGWCC 3 cut(s) 33, 1066, 2213
Eco53kI GAGCTC 1 cut(s) 927
Eco57I CTGAAG 4 cut(s) 1128, 1152, 1311, 2421
Eco72I CACGTG 1 cut(s) 2933
Eco88I CYCGRG 2 cut(s) 60, 1854
EcoICRI GAGCTC 1 cut(s) 927
EcoNI CCTNNNNNAGG 1 cut(s) 2316
EcoO109I RGGNCCY 2 cut(s) 33, 2213
EcoRI GAATTC 3 cut(s) 411, 1759, 1863
EcoRV GATATC 1 cut(s) 2672
EcoT14I CCWWGG 3 cut(s) 28, 2373, 3171
EcoT22I ATGCAT 2 cut(s) 697, 1834
EcoT38I GRGCYC 1 cut(s) 929
ErhI CCWWGG 3 cut(s) 28, 2373, 3171
Esp3I CGTCTC 1 cut(s) 1227
FaqI GGGAC 2 cut(s) 1372, 2477
FbaI TGATCA 1 cut(s) 3186
Fnu4HI GCNGC 9 cut(s) 435, 458, 2121, 2277, 2280, 2283, 2706, 3193, 3196
FriOI GRGCYC 1 cut(s) 929
Fsp4HI GCNGC 9 cut(s) 435, 458, 2121, 2277, 2280, 2283, 2706, 3193, 3196
FspBI CTAG 6 cut(s) 489, 1343, 1764, 2730, 2783, 2976
GlaI GCGC 2 cut(s) 197, 2582
GluI GCNGC 9 cut(s) 435, 458, 2121, 2277, 2280, 2283, 2706, 3193, 3196
HaeIII GGCC 4 cut(s) 437, 457, 679, 2378
HapII CCGG 4 cut(s) 1069, 1332, 1355, 1736
HgaI GACGC 1 cut(s) 3212
HhaI GCGC 2 cut(s) 198, 2583
Hin6I GCGC 2 cut(s) 196, 2581
HinP1I GCGC 2 cut(s) 196, 2581
HindIII AAGCTT 1 cut(s) 2433
HpaII CCGG 4 cut(s) 1069, 1332, 1355, 1736
HphI GGTGA 6 cut(s) 61, 461, 555, 737, 794, 2504
Hpy166II GTNNAC 9 cut(s) 222, 280, 607, 1285, 1926, 2132, 2243, 2936, 3107
Hpy8I GTNNAC 9 cut(s) 222, 280, 607, 1285, 1926, 2132, 2243, 2936, 3107
Hpy99I CGWCG 2 cut(s) 908, 2241
HpyCH4IV ACGT 5 cut(s) 469, 557, 2009, 2110, 2932
HpyF10VI GCNNNNNNNGC 7 cut(s) 498, 1829, 1900, 2129, 2282, 2384, 3217
HpySE526I ACGT 5 cut(s) 469, 557, 2009, 2110, 2932
HspAI GCGC 2 cut(s) 196, 2581
Ksp22I TGATCA 1 cut(s) 3186
Kzo9I GATC 5 cut(s) 66, 1615, 1650, 2206, 3186
LguI GCTCTTC 3 cut(s) 1236, 1635, 3150
LmnI GCTCC 5 cut(s) 373, 498, 2477, 2509, 2920
Lsp1109I GCAGC 7 cut(s) 2107, 2263, 2266, 2294, 2692, 3204, 3207
MabI ACCWGGT 2 cut(s) 507, 2455
MaeI CTAG 6 cut(s) 489, 1343, 1764, 2730, 2783, 2976
MaeII ACGT 5 cut(s) 469, 557, 2009, 2110, 2932
MalI GATC 5 cut(s) 68, 1617, 1652, 2208, 3188
MboI GATC 5 cut(s) 66, 1615, 1650, 2206, 3186
MfeI CAATTG 2 cut(s) 1608, 1986
MhlI GDGCHC 8 cut(s) 282, 326, 929, 1287, 1630, 2134, 2474, 2917
MluI ACGCGT 1 cut(s) 3204
MmeI TCCRAC 1 cut(s) 3011
Mph1103I ATGCAT 2 cut(s) 697, 1834
MroXI GAANNNNTTC 4 cut(s) 1037, 1749, 2434, 2631
MslI CAYNNNNRTG 5 cut(s) 183, 275, 443, 2238, 2541
MspCI CTTAAG 1 cut(s) 335
MspI CCGG 4 cut(s) 1069, 1332, 1355, 1736
MunI CAATTG 2 cut(s) 1608, 1986
Mva1269I GAATGC 1 cut(s) 3009
MvnI CGCG 3 cut(s) 198, 1722, 3206
MwoI GCNNNNNNNGC 7 cut(s) 498, 1829, 1900, 2129, 2282, 2384, 3217
NciI CCSGG 2 cut(s) 1333, 1356
NdeII GATC 5 cut(s) 66, 1615, 1650, 2206, 3186
NheI GCTAGC 1 cut(s) 488
NlaIV GGNNCC 5 cut(s) 10, 34, 1776, 2215, 2825
NmeAIII GCCGAG 1 cut(s) 2200
NmuCI GTSAC 5 cut(s) 92, 549, 1394, 1566, 1638
NsiI ATGCAT 2 cut(s) 697, 1834
NspI RCATGY 3 cut(s) 274, 3030, 3041
NspV TTCGAA 1 cut(s) 1267
OliI CACNNNNGTG 1 cut(s) 2238
PaeI GCATGC 1 cut(s) 3041
PaeR7I CTCGAG 1 cut(s) 1854
PagI TCATGA 1 cut(s) 1543
PaqCI CACCTGC 1 cut(s) 577
PasI CCCWGGG 1 cut(s) 494
PciI ACATGT 2 cut(s) 270, 3026
PciSI GCTCTTC 3 cut(s) 1236, 1635, 3150
PcsI WCGNNNNNNNCGW 2 cut(s) 86, 2015
PctI GAATGC 1 cut(s) 3009
PdmI GAANNNNTTC 4 cut(s) 1037, 1749, 2434, 2631
PfeI GAWTC 5 cut(s) 750, 812, 971, 2333, 3018
PfoI TCCNGGA 1 cut(s) 2302
PkrI GCNGC 9 cut(s) 436, 459, 2122, 2278, 2281, 2284, 2707, 3194, 3197
PmaCI CACGTG 1 cut(s) 2933
PmlI CACGTG 1 cut(s) 2933
Ppu21I YACGTR 1 cut(s) 2933
PpuMI RGGWCCY 2 cut(s) 33, 2213
PscI ACATGT 2 cut(s) 270, 3026
Psp124BI GAGCTC 1 cut(s) 929
Psp5II RGGWCCY 2 cut(s) 33, 2213
PspCI CACGTG 1 cut(s) 2933
PspN4I GGNNCC 5 cut(s) 10, 34, 1776, 2215, 2825
PspPI GGNCC 4 cut(s) 33, 677, 1066, 2213
PspPPI RGGWCCY 2 cut(s) 33, 2213
PstI CTGCAG 2 cut(s) 2284, 2802
PstNI CAGNNNCTG 1 cut(s) 2180
RsaI GTAC 9 cut(s) 173, 221, 468, 1306, 1330, 2244, 2251, 2714, 3106
RsaNI GTAC 9 cut(s) 172, 220, 467, 1305, 1329, 2243, 2250, 2713, 3105
RseI CAYNNNNRTG 5 cut(s) 183, 275, 443, 2238, 2541
SacI GAGCTC 1 cut(s) 929
SapI GCTCTTC 3 cut(s) 1236, 1635, 3150
SatI GCNGC 9 cut(s) 435, 458, 2121, 2277, 2280, 2283, 2706, 3193, 3196
Sau3AI GATC 5 cut(s) 66, 1615, 1650, 2206, 3186
Sau96I GGNCC 4 cut(s) 33, 677, 1066, 2213
SduI GDGCHC 8 cut(s) 282, 326, 929, 1287, 1630, 2134, 2474, 2917
SexAI ACCWGGT 2 cut(s) 507, 2455
SfcI CTRYAG 5 cut(s) 1512, 1974, 2259, 2280, 2798
Sfr274I CTCGAG 1 cut(s) 1854
SfuI TTCGAA 1 cut(s) 1267
SinI GGWCC 3 cut(s) 33, 1066, 2213
SlaI CTCGAG 1 cut(s) 1854
SmiMI CAYNNNNRTG 5 cut(s) 183, 275, 443, 2238, 2541
SmlI CTYRAG 4 cut(s) 335, 1854, 1980, 2942
SmoI CTYRAG 4 cut(s) 335, 1854, 1980, 2942
SphI GCATGC 1 cut(s) 3041
SsiI CCGC 6 cut(s) 25, 371, 434, 458, 1901, 2224
SspI AATATT 2 cut(s) 525, 3255
SspMI CTAG 6 cut(s) 489, 1343, 1764, 2730, 2783, 2976
SstI GAGCTC 1 cut(s) 929
StyI CCWWGG 3 cut(s) 28, 2373, 3171
TaiI ACGT 5 cut(s) 472, 560, 2012, 2113, 2935
TaqI TCGA 7 cut(s) 89, 214, 923, 1212, 1267, 1855, 1967
TatI WGTACW 3 cut(s) 171, 219, 2249
TauI GCSGC 2 cut(s) 437, 460
TfiI GAWTC 5 cut(s) 750, 812, 971, 2333, 3018
TscAI CASTG 4 cut(s) 106, 282, 1005, 2259
TseFI GTSAC 5 cut(s) 92, 549, 1394, 1566, 1638
TseI GCWGC 7 cut(s) 2120, 2276, 2279, 2282, 2705, 3192, 3195
Tsp45I GTSAC 5 cut(s) 92, 549, 1394, 1566, 1638
TspGWI ACGGA 4 cut(s) 1268, 1896, 2464, 3142
TspRI CASTG 4 cut(s) 106, 282, 1005, 2259
Vha464I CTTAAG 1 cut(s) 335
VneI GTGCAC 3 cut(s) 278, 1283, 2130
VpaK11BI GGWCC 3 cut(s) 33, 1066, 2213
XagI CCTNNNNNAGG 1 cut(s) 2316
XceI RCATGY 3 cut(s) 274, 3030, 3041
XhoI CTCGAG 1 cut(s) 1854
XmnI GAANNNNTTC 4 cut(s) 1037, 1749, 2434, 2631
XspI CTAG 6 cut(s) 489, 1343, 1764, 2730, 2783, 2976
Zsp2I ATGCAT 2 cut(s) 697, 1834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.