Rmu_sc0000319.1_g000011

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000319.1
OG0012264
Physical Location & Seq
Reverse (-)
56249 .. 73686
17438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000319.1_g000011.1.cds

Sequence Viewer

Length: 4221 bp
atgacggtgtcccaggatatggtggcggtgatgttgcagtttatagagtcatggcagggcggagacagttcagcaaggtgcagtgcaacgcttgttgtggtggctacggaggacgctgttcatgggaatataactaaggatcaaggcatctcttggacctcccaacctcgtctattctatgagatttcgccgacgtcaatgacaattgtcatctctgttgaaggcacagtgggtaccaatggtgcaactaggagtcagtttcttggaggcctgttgcttgaagttttggcaggacggttcggcagcagtttcggatgtgaccactctaaagcatggtggttgggttggtttgggtctgcaattgtctgtgatggattgggaggggggttggatctcggtccactttgcttccaagttgcgacgaggcttctatggttgcatcaatgtggtaatctgggtgatctcaagtcgatttgtgtttggtttgctttggttttcaagcggttgaggctcttcgtagtcggtgttgtttcgtcgatgacatccgggattggctactacttgcttatggcggtgctggatcggctgtgccactatgcacaggagatgaatcccaaactctcttacatttctccccaaattccccgatcgactctgcaattcacttcgcttctaaacgccagcttgatcgacgaccaagaacaaagtcgtctctccgggtccggattacgatttccgggttctgctctgaagaaatgtcttggcgcggttgatgatttgggttctgattggactgtgagttttggattgggggtggaaaacaaggtggctagggtttataatggtcgattgagaatggtgaggatactgggactgactcggggtgagacggacgagtcgccgcgggagatcacctcgcggaccaacatatcgagtgatgccggcgagaatgggtggctgatacgcttcttcgactcggcgttcttctgcgagtggatcgccgtgagctacctgtacaagcatgaacactccggggttcgagactatctctgtaatcgaatgtacactctgccattgccaggtattgagagctatttgtttcagatatgttatatgtccgtacacaaacccagcccctctttggataagtttgtcattgatatgtgctctaagtcccttaagatggctctcaaggtgcattggtttttgctggccgagctcgaagattccgatgacaatgaagggattagtaggatacaggagaagtgtcagattgctgctactttgatgggtgagtgggcgccgctggttcggcctcagagtgaagctagtagccccgggagtaagaaccaggtgttgaatagactgttttcatctaagcagaagctgttgtcattgacgtcttcgcctccttctcagaggtctttttcattctcgccttcatcgggaaacagtgtgcaagaggatggtggagggcagttgtctcctgatgagaacaagatttttaagaagttcattccgggtccgaaggttagggatgcattgctgtttaggaagtcggtggagaaagatgaggatgactctgagaaggatgggttctttaagaggctgctaagggacagtagaggtgatgatgagactgggtcgaagcgtagagattctttgctatttaggaagtcatcggagaaggatgatgatgatacagaaaaggatgggttctttaagagattattaagggatagtaaaggggacgatgaagaattaacttctagttcagaggggttttttaagaaattgtttcgtgaaagtaagagcgattccgaggataaatcagtttctaaatcagtggatgatgaagaaaaagatgggttctttaagaagtttttcaaagagaaatttgaggacaaaaaggatagaaatgaagatgatgatacagcacagtcagaaggaaaatgctcgaaatcagctgaagatgatgagaaagatgggtttttccgaaagttatttagtaacaagtttgaggacagaaaagatgggaatgataaaactgaggaagggagtgccaatggggaggacgaagagcattctgacttttcattattccgaagattattccgcgtgcatcctgaagatggcaaaagtattgctaatgaaagcaccaatggtagcagcttgttagaaagtagtccaggcactgagaattttttccgtaaattgtttagagatcgagaccggtcagttgaagattcagagctatttggttcaaaaaaacataaagagaaacatcctgattcaccaaagcaacaaaacgaaaagtcaagtgcaaaacccccacttccaagtaattcagcatcccattaccgtaaaggggcataccatgagtcattggagtttgtgcagtcactatgtgagacgtcatatggtttagtggatatatttccaattgaagatcgcaaaagtgcacttcgtgaggtttatacttatcttgcttatattttgtcactcgcagagatcaatttgcacattgccaaggctcaaaataatggaggagttggctttccaatggggaaggggatgtatcgcgtggtgtatataccagaagatgaagctgttcttttgaattctagggagaaggcaccctacctcatctgtgttgaagtcttaaaaagtgaaattccaagcaatccaaaggatacatctagctctcaaaagctctctcgaggaggaattcctttggcaaatggagatgcacttctgccaagaccacctccctgggcatatcctttgtggactgtccaagaggtctatcgcaatagtaatgataggatgtcaagttctactgctcatgcaattgaccaggcaatgtcacatacgccagaggcaaaaaagaaatttgttactctgaaaatatctgtggagaagaagttgcctagccagactctgaaaatagagagtatatcaaacagtagtagaggagaggctcttaattgtgaatcaaaagaagcacgagggagtgatttggagtgggtaagggtggtgcttacagcagatcctggttttagaatggaggacattgaggatcaaggaccatctcgacggaaggaacaccgtcgtgttccaagtacagtggctatagaagaagtaaaggctgctgcagcaaaaggagaagcacctcctggactccctttgaaaggggctggtcaggattcatcagatgcacagccaacagctaatggtggtactcctaaggccagcgatgcgttgtctggtgaactttgggagataaagaaggagagaatacggaagtcttcagtttatggaaagttacctggttgggacttgcgctctgtcattgtgaagagtggtgatgattgtagacaggagcaccttgctgtgcagcttatttctcacttctacgatatattccaagaagctgggcttcccctctggttgcgaccttatgaagtcttagttacttcttcttacacagctcttattgaaacaattccagatacggcttcacttcattctatcaaaagtagacatcccaacatcactagcttgcgacacttctttgatgctaagtatcaagaaaattctccaacttttaaacttgcccagagaaattttgttgaaagtatggctggatattccttagtgtgctaccttctgcaggtgaaagataggcacaatggaaacctcttaatggatgaagaaggtcatattatacatattgattttggcttcatgctctccaattcacctgggggtgtaaattttgagagcgctccattcaagttgacacgtgaacttcttgaggttatggactctgatgctgaaggagttccaagcgagttcttcgattactttaaagttttgtgcattcaagggttccttacatgtcggaagcatgcggagcgtattattcttcttgtagagatgttgcaggactctggtttcccatgctttaaaggtggtccacggacaatacagaatctaaggaaacgatttcacctgagtttgacagaagagcaatgtgtgtccttggtgctttccctaatcagcagcagcttggatgcttggcggacacgtcagtggtccacggacaatacagaatctaaggaaacgatttcacctgagtttgacagaagagcaatgtgtgtccttggtgctttccctaatcagcagcagcttggatgcttggtggacacgtcagtatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012506 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017137 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0030258 GO:0030659 GO:0031090 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0035618 GO:0035619 GO:0040007 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044706 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048015 GO:0048017 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051704 GO:0051716 GO:0052742 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0097708 GO:0099402 GO:0120025 GO:0120038 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

1406

Amino Acids

157.3

Weight (kDa)

5.83

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09350 AT5G64070 AT5G64070
fragaria_vesca FvH4_1g24240
malus_domestica MD09G1261800.v1.1 MD17G1256600.v1.1
prunus_persica Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1
pyrus_communis pycom09g17610 pycom17g25880
rosa_chinensis RchiOBHm_Chr2g0120731
rosa_laevigata RLG00000018575
rosa_multiflora Rmu_sc0000319.1_g000011
rosa_roxburghii Rroxscaffold_2G00123200
rosa_rugosa Rorug02G0230500
rosa_samantha Rh2BG298500 Rh2CG276200
rosa_wichuraiana Rw2G023030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
AarI CACCTGC 1 cut(s) 3664
AasI GACNNNNNNGTC 1 cut(s) 895
AatII GACGTC 3 cut(s) 197, 1403, 2417
Acc36I ACCTGC 1 cut(s) 3664
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 3 cut(s) 233, 1300, 2635
AccB7I CCANNNNNTGG 1 cut(s) 19
AccI GTMKAC 2 cut(s) 3376, 3541
AccII CGCG 5 cut(s) 767, 904, 919, 2118, 2583
AccIII TCCGGA 1 cut(s) 722
AclWI GGATC 6 cut(s) 147, 399, 588, 1004, 3044, 3087
AcoI YGGCCR 1 cut(s) 1212
AcuI CTGAAG 5 cut(s) 770, 1989, 2148, 3294, 3858
AcvI CACGTG 1 cut(s) 3806
AcyI GRCGYC 4 cut(s) 194, 1301, 1400, 2414
AdeI CACNNNGTG 2 cut(s) 2408, 2468
AfaI GTAC 6 cut(s) 235, 1016, 1064, 1122, 3124, 3241
AfeI AGCGCT 1 cut(s) 3787
AflII CTTAAG 1 cut(s) 1178
AflIII ACRYGT 4 cut(s) 3803, 3899, 4088, 4209
AgeI ACCGGT 1 cut(s) 2232
AjiI CACGTC 2 cut(s) 4091, 4212
AleI CACNNNNGTG 2 cut(s) 3111, 4093
AloI GAACNNNNNNTCC 2 cut(s) 2818, 2850
Alw21I GWGCWC 4 cut(s) 1169, 1221, 2464, 3387
Alw26I GTCTC 8 cut(s) 57, 716, 881, 1035, 1488, 1631, 2223, 2405
Alw44I GTGCAC 1 cut(s) 2460
AlwI GGATC 6 cut(s) 147, 399, 588, 1004, 3044, 3087
AlwNI CAGNNNCTG 4 cut(s) 1387, 2195, 2941, 3053
Ama87I CYCGRG 3 cut(s) 879, 1337, 2718
Aor13HI TCCGGA 1 cut(s) 722
Aor51HI AGCGCT 1 cut(s) 3787
AoxI GGCC 4 cut(s) 268, 1212, 1313, 3249
ApaLI GTGCAC 1 cut(s) 2460
AsiGI ACCGGT 1 cut(s) 2232
Asp700I GAANNNNTTC 5 cut(s) 1883, 2204, 2610, 3504, 3843
Asp718I GGTACC 1 cut(s) 233
AspLEI GCGC 4 cut(s) 767, 1303, 3345, 3788
AspS9I GGNCC 8 cut(s) 156, 398, 720, 921, 1523, 3086, 3977, 4098
AsuC2I CCSGG 7 cut(s) 547, 718, 738, 1033, 1338, 1339, 1521
AvaI CYCGRG 3 cut(s) 879, 1337, 2718
AvaII GGWCC 8 cut(s) 156, 398, 720, 921, 1523, 3086, 3977, 4098
AxyI CCTNAGG 1 cut(s) 3246
BaeGI GKGCMC 1 cut(s) 2464
BanI GGYRCC 3 cut(s) 233, 1300, 2635
BanII GRGCYC 1 cut(s) 1221
BarI GAAGNNNNNNTAC 2 cut(s) 2624, 2656
BauI CACGAG 1 cut(s) 3006
BbrPI CACGTG 1 cut(s) 3806
BbsI GAAGAC 2 cut(s) 1395, 3300
Bbv12I GWGCWC 4 cut(s) 1169, 1221, 2464, 3387
BceAI ACGGC 2 cut(s) 986, 3531
BcgI CGANNNNNNTGC 2 cut(s) 292, 326
BciVI GTATCC 3 cut(s) 858, 1248, 2686
BcnI CCSGG 7 cut(s) 547, 718, 738, 1033, 1338, 1339, 1521
BcoDI GTCTC 8 cut(s) 57, 716, 881, 1035, 1488, 1631, 2223, 2405
BfaI CTAG 8 cut(s) 249, 831, 1329, 1770, 2625, 2700, 2931, 3558
BfmI CTRYAG 3 cut(s) 3132, 3153, 3671
BfoI RGCGCY 2 cut(s) 1304, 3789
BfrI CTTAAG 1 cut(s) 1178
BfuAI ACCTGC 1 cut(s) 3664
BfuI GTATCC 3 cut(s) 858, 1248, 2686
Bme18I GGWCC 8 cut(s) 156, 398, 720, 921, 1523, 3086, 3977, 4098
BmeT110I CYCGRG 3 cut(s) 879, 1337, 2718
BmgBI CACGTC 2 cut(s) 4091, 4212
BmgT120I GGNCC 8 cut(s) 156, 398, 720, 921, 1523, 3086, 3977, 4098
BmiI GGNNCC 6 cut(s) 235, 721, 1302, 1524, 2637, 3893
BmrI ACTGGG 2 cut(s) 878, 1650
BmuI ACTGGG 2 cut(s) 878, 1650
BoxI GACNNNNGTC 1 cut(s) 206
BpiI GAAGAC 2 cut(s) 1395, 3300
BplI GAGNNNNNCTC 6 cut(s) 899, 931, 1032, 1064, 1565, 1597
Bpu10I CCTNAGC 1 cut(s) 1613
BpuEI CTTGAG 3 cut(s) 449, 1175, 3836
BpuMI CCSGG 7 cut(s) 547, 718, 738, 1033, 1338, 1339, 1521
BsaAI YACGTR 1 cut(s) 3806
BsaHI GRCGYC 4 cut(s) 194, 1301, 1400, 2414
BsaI GGTCTC 1 cut(s) 2223
BsaWI WCCGGW 2 cut(s) 722, 2232
BsaXI ACNNNNNCTCC 2 cut(s) 2909, 2939
Bse118I RCCGGY 2 cut(s) 941, 2232
Bse1I ACTGG 2 cut(s) 873, 1645
Bse21I CCTNAGG 1 cut(s) 3246
Bse3DI GCAATG 6 cut(s) 1073, 1541, 2523, 2868, 4040, 4161
BseAI TCCGGA 1 cut(s) 722
BseMI GCAATG 6 cut(s) 1073, 1541, 2523, 2868, 4040, 4161
BseMII CTCAG 7 cut(s) 1331, 1430, 1575, 2040, 2187, 4007, 4128
BseNI ACTGG 2 cut(s) 873, 1645
BseRI GAGGAG 3 cut(s) 2562, 2736, 2988
BseSI GKGCMC 1 cut(s) 2464
BseYI CCCAGC 2 cut(s) 1130, 3434
BsgI GTGCAG 3 cut(s) 100, 2417, 3416
Bsh1236I CGCG 5 cut(s) 767, 904, 919, 2118, 2583
Bsh1285I CGRYCG 1 cut(s) 650
BshFI GGCC 4 cut(s) 270, 1214, 1315, 3251
BshNI GGYRCC 3 cut(s) 233, 1300, 2635
BshTI ACCGGT 1 cut(s) 2232
BsiEI CGRYCG 1 cut(s) 650
BsiHKAI GWGCWC 4 cut(s) 1169, 1221, 2464, 3387
BsiHKCI CYCGRG 3 cut(s) 879, 1337, 2718
BsiSI CCGG 9 cut(s) 546, 717, 723, 737, 942, 1032, 1338, 1520, 2233
BslFI GGGAC 5 cut(s) 885, 1159, 1631, 1763, 3350
BsmAI GTCTC 8 cut(s) 57, 716, 881, 1035, 1488, 1631, 2223, 2405
BsmBI CGTCTC 3 cut(s) 716, 881, 2405
BsmFI GGGAC 5 cut(s) 885, 1159, 1631, 1763, 3350
BsmI GAATGC 2 cut(s) 2083, 3882
BsnI GGCC 4 cut(s) 270, 1214, 1315, 3251
Bso31I GGTCTC 1 cut(s) 2223
BsoBI CYCGRG 3 cut(s) 879, 1337, 2718
Bsp1286I GDGCHC 4 cut(s) 1169, 1221, 2464, 3387
Bsp13I TCCGGA 1 cut(s) 722
Bsp1407I TGTACA 2 cut(s) 1014, 1062
BspANI GGCC 4 cut(s) 270, 1214, 1315, 3251
BspCNI CTCAG 7 cut(s) 1330, 1429, 1576, 2041, 2188, 4008, 4129
BspEI TCCGGA 1 cut(s) 722
BspFNI CGCG 5 cut(s) 767, 904, 919, 2118, 2583
BspLI GGNNCC 6 cut(s) 235, 721, 1302, 1524, 2637, 3893
BspMAI CTGCAG 2 cut(s) 3157, 3675
BspMI ACCTGC 1 cut(s) 3664
BspPI GGATC 6 cut(s) 147, 399, 588, 1004, 3044, 3087
BspQI GCTCTTC 4 cut(s) 518, 2073, 4023, 4144
BspT107I GGYRCC 3 cut(s) 233, 1300, 2635
BspTI CTTAAG 1 cut(s) 1178
BspTNI GGTCTC 1 cut(s) 2223
BsrDI GCAATG 6 cut(s) 1073, 1541, 2523, 2868, 4040, 4161
BsrFI RCCGGY 2 cut(s) 941, 2232
BsrGI TGTACA 2 cut(s) 1014, 1062
BsrI ACTGG 2 cut(s) 873, 1645
BssAI RCCGGY 2 cut(s) 941, 2232
BssNI GRCGYC 4 cut(s) 194, 1301, 1400, 2414
BssSI CACGAG 1 cut(s) 3006
BssT1I CCWWGG 3 cut(s) 2529, 4044, 4165
Bst2BI CACGAG 1 cut(s) 3006
Bst6I CTCTTC 5 cut(s) 518, 2073, 3353, 4023, 4144
BstACI GRCGYC 4 cut(s) 194, 1301, 1400, 2414
BstAFI CTTAAG 1 cut(s) 1178
BstAUI TGTACA 2 cut(s) 1014, 1062
BstBAI YACGTR 1 cut(s) 3806
BstC8I GCNNGC 7 cut(s) 682, 943, 1212, 2120, 3253, 3563, 3912
BstDSI CCRYGG 3 cut(s) 902, 3980, 4101
BstENI CCTNNNNNAGG 2 cut(s) 3189, 3671
BstFNI CGCG 5 cut(s) 767, 904, 919, 2118, 2583
BstH2I RGCGCY 2 cut(s) 1304, 3789
BstHHI GCGC 4 cut(s) 767, 1303, 3345, 3788
BstMAI GTCTC 8 cut(s) 57, 716, 881, 1035, 1488, 1631, 2223, 2405
BstMCI CGRYCG 1 cut(s) 650
BstMWI GCNNNNNNNGC 7 cut(s) 508, 583, 1216, 1312, 3155, 3257, 3916
BstNSI RCATGY 2 cut(s) 3903, 3914
BstPAI GACNNNNGTC 1 cut(s) 206
BstSFI CTRYAG 3 cut(s) 3132, 3153, 3671
BstSLI GKGCMC 1 cut(s) 2464
BstUI CGCG 5 cut(s) 767, 904, 919, 2118, 2583
BstV2I GAAGAC 2 cut(s) 1395, 3300
BstX2I RGATCY 2 cut(s) 391, 3049
BstXI CCANNNNNNTGG 2 cut(s) 2772, 3434
BstYI RGATCY 2 cut(s) 391, 3049
Bsu36I CCTNAGG 1 cut(s) 3246
BsuI GTATCC 3 cut(s) 858, 1248, 2686
BsuRI GGCC 4 cut(s) 270, 1214, 1315, 3251
BtgI CCRYGG 3 cut(s) 902, 3980, 4101
BtgZI GCGATG 1 cut(s) 3270
BtrI CACGTC 2 cut(s) 4091, 4212
BtsI GCAGTG 1 cut(s) 88
BtsIMutI CAGTG 7 cut(s) 88, 234, 1459, 1851, 2193, 3132, 4100
BveI ACCTGC 1 cut(s) 3664
Cac8I GCNNGC 7 cut(s) 682, 943, 1212, 2120, 3253, 3563, 3912
CaiI CAGNNNCTG 4 cut(s) 1387, 2195, 2941, 3053
CfoI GCGC 4 cut(s) 767, 1303, 3345, 3788
Cfr10I RCCGGY 2 cut(s) 941, 2232
Cfr13I GGNCC 8 cut(s) 156, 398, 720, 921, 1523, 3086, 3977, 4098
Cfr42I CCGCGG 1 cut(s) 905
Cfr9I CCCGGG 1 cut(s) 1337
CseI GACGC 1 cut(s) 122
CsiI ACCWGGT 2 cut(s) 1350, 3328
Csp6I GTAC 6 cut(s) 234, 1015, 1063, 1121, 3123, 3240
CspAI ACCGGT 1 cut(s) 2232
CspCI CAANNNNNGTGG 2 cut(s) 3108, 3143
CviQI GTAC 6 cut(s) 234, 1015, 1063, 1121, 3123, 3240
DinI GGCGCC 1 cut(s) 1302
DraI TTTAAA 3 cut(s) 3610, 3871, 3970
DraIII CACNNNGTG 2 cut(s) 2408, 2468
DrdI GACNNNNNNGTC 1 cut(s) 895
DseDI GACNNNNNNGTC 1 cut(s) 895
EaeI YGGCCR 1 cut(s) 1212
Eam1104I CTCTTC 5 cut(s) 518, 2073, 3353, 4023, 4144
EarI CTCTTC 5 cut(s) 518, 2073, 3353, 4023, 4144
EciI GGCGGA 2 cut(s) 75, 4099
Ecl136II GAGCTC 1 cut(s) 1219
Eco130I CCWWGG 3 cut(s) 2529, 4044, 4165
Eco147I AGGCCT 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 1221
Eco31I GGTCTC 1 cut(s) 2223
Eco47I GGWCC 8 cut(s) 156, 398, 720, 921, 1523, 3086, 3977, 4098
Eco47III AGCGCT 1 cut(s) 3787
Eco53kI GAGCTC 1 cut(s) 1219
Eco57I CTGAAG 5 cut(s) 770, 1989, 2148, 3294, 3858
Eco72I CACGTG 1 cut(s) 3806
Eco81I CCTNAGG 1 cut(s) 3246
Eco88I CYCGRG 3 cut(s) 879, 1337, 2718
EcoICRI GAGCTC 1 cut(s) 1219
EcoNI CCTNNNNNAGG 2 cut(s) 3189, 3671
EcoRI GAATTC 2 cut(s) 2620, 2727
EcoT14I CCWWGG 3 cut(s) 2529, 4044, 4165
EcoT22I ATGCAT 1 cut(s) 1543
EcoT38I GRGCYC 1 cut(s) 1221
EgeI GGCGCC 1 cut(s) 1302
EheI GGCGCC 1 cut(s) 1302
ErhI CCWWGG 3 cut(s) 2529, 4044, 4165
Esp3I CGTCTC 3 cut(s) 716, 881, 2405
FalI AAGNNNNNCTT 6 cut(s) 2598, 2630, 3423, 3455, 3879, 3911
FaqI GGGAC 5 cut(s) 885, 1159, 1631, 1763, 3350
FauI CCCGC 1 cut(s) 897
FauNDI CATATG 1 cut(s) 2419
FblI GTMKAC 2 cut(s) 3376, 3541
FriOI GRGCYC 1 cut(s) 1221
FspBI CTAG 8 cut(s) 249, 831, 1329, 1770, 2625, 2700, 2931, 3558
GlaI GCGC 4 cut(s) 766, 1302, 3344, 3787
GsaI CCCAGC 2 cut(s) 1134, 3438
HaeII RGCGCY 2 cut(s) 1304, 3789
HaeIII GGCC 4 cut(s) 270, 1214, 1315, 3251
HapII CCGG 9 cut(s) 546, 717, 723, 737, 942, 1032, 1338, 1520, 2233
HgaI GACGC 1 cut(s) 122
HhaI GCGC 4 cut(s) 767, 1303, 3345, 3788
Hin1I GRCGYC 4 cut(s) 194, 1301, 1400, 2414
Hin6I GCGC 4 cut(s) 765, 1301, 3343, 3786
HinP1I GCGC 4 cut(s) 765, 1301, 3343, 3786
HincII GTYRAC 1 cut(s) 3801
HindII GTYRAC 1 cut(s) 3801
HpaII CCGG 9 cut(s) 546, 717, 723, 737, 942, 1032, 1338, 1520, 2233
Hpy99I CGWCG 6 cut(s) 196, 424, 538, 695, 3099, 3114
HpyCH4IV ACGT 6 cut(s) 194, 1400, 2414, 3805, 4090, 4211
HpyF10VI GCNNNNNNNGC 7 cut(s) 508, 583, 1216, 1312, 3155, 3257, 3916
HpySE526I ACGT 6 cut(s) 194, 1400, 2414, 3805, 4090, 4211
Hsp92I GRCGYC 4 cut(s) 194, 1301, 1400, 2414
HspAI GCGC 4 cut(s) 765, 1301, 3343, 3786
KasI GGCGCC 1 cut(s) 1300
Kpn2I TCCGGA 1 cut(s) 722
KpnI GGTACC 1 cut(s) 237
KroI GCCGGC 1 cut(s) 941
KroNI GCCGGC 1 cut(s) 943
KspI CCGCGG 1 cut(s) 905
LguI GCTCTTC 4 cut(s) 518, 2073, 4023, 4144
LmnI GCTCC 3 cut(s) 3382, 3793, 3916
MabI ACCWGGT 2 cut(s) 1350, 3328
MaeI CTAG 8 cut(s) 249, 831, 1329, 1770, 2625, 2700, 2931, 3558
MaeII ACGT 6 cut(s) 194, 1400, 2414, 3805, 4090, 4211
MaeIII GTNAC 8 cut(s) 317, 2009, 2400, 2499, 2865, 2896, 3324, 3472
MfeI CAATTG 4 cut(s) 204, 360, 2442, 2850
MflI RGATCY 2 cut(s) 391, 3049
MhlI GDGCHC 4 cut(s) 1169, 1221, 2464, 3387
Mly113I GGCGCC 1 cut(s) 1301
MmeI TCCRAC 3 cut(s) 369, 3626, 3884
Mph1103I ATGCAT 1 cut(s) 1543
MroI TCCGGA 1 cut(s) 722
MroNI GCCGGC 1 cut(s) 941
MroXI GAANNNNTTC 5 cut(s) 1883, 2204, 2610, 3504, 3843
MslI CAYNNNNRTG 4 cut(s) 444, 1160, 3111, 4093
MspA1I CMGCKG 3 cut(s) 904, 1306, 1967
MspCI CTTAAG 1 cut(s) 1178
MspI CCGG 9 cut(s) 546, 717, 723, 737, 942, 1032, 1338, 1520, 2233
MunI CAATTG 4 cut(s) 204, 360, 2442, 2850
Mva1269I GAATGC 2 cut(s) 2083, 3882
MvnI CGCG 5 cut(s) 767, 904, 919, 2118, 2583
MwoI GCNNNNNNNGC 7 cut(s) 508, 583, 1216, 1312, 3155, 3257, 3916
NaeI GCCGGC 1 cut(s) 943
NarI GGCGCC 1 cut(s) 1301
NciI CCSGG 7 cut(s) 547, 718, 738, 1033, 1338, 1339, 1521
NdeI CATATG 1 cut(s) 2419
NgoMIV GCCGGC 1 cut(s) 941
NlaIV GGNNCC 6 cut(s) 235, 721, 1302, 1524, 2637, 3893
NmeAIII GCCGAG 2 cut(s) 956, 1240
NmuCI GTSAC 4 cut(s) 317, 2400, 2499, 2865
NsiI ATGCAT 1 cut(s) 1543
NspI RCATGY 2 cut(s) 3903, 3914
OliI CACNNNNGTG 2 cut(s) 3111, 4093
PaeI GCATGC 1 cut(s) 3914
PaeR7I CTCGAG 1 cut(s) 2718
PaqCI CACCTGC 1 cut(s) 3664
PasI CCCWGGG 1 cut(s) 2772
PceI AGGCCT 1 cut(s) 270
PciI ACATGT 1 cut(s) 3899
PciSI GCTCTTC 4 cut(s) 518, 2073, 4023, 4144
PcsI WCGNNNNNNNCGW 1 cut(s) 1227
PctI GAATGC 2 cut(s) 2083, 3882
PdiI GCCGGC 1 cut(s) 943
PdmI GAANNNNTTC 5 cut(s) 1883, 2204, 2610, 3504, 3843
PflFI GACNNNGTC 2 cut(s) 7, 1642
PflMI CCANNNNNTGG 1 cut(s) 19
PfoI TCCNGGA 2 cut(s) 545, 3175
PinAI ACCGGT 1 cut(s) 2232
Ple19I CGATCG 1 cut(s) 650
PluTI GGCGCC 1 cut(s) 1304
PmaCI CACGTG 1 cut(s) 3806
PmlI CACGTG 1 cut(s) 3806
Ppu21I YACGTR 1 cut(s) 3806
PscI ACATGT 1 cut(s) 3899
PshAI GACNNNNGTC 1 cut(s) 206
PsiI TTATAA 1 cut(s) 840
Psp124BI GAGCTC 1 cut(s) 1221
PspCI CACGTG 1 cut(s) 3806
PspFI CCCAGC 2 cut(s) 1130, 3434
PspN4I GGNNCC 6 cut(s) 235, 721, 1302, 1524, 2637, 3893
PspPI GGNCC 8 cut(s) 156, 398, 720, 921, 1523, 3086, 3977, 4098
PstI CTGCAG 2 cut(s) 3157, 3675
PstNI CAGNNNCTG 4 cut(s) 1387, 2195, 2941, 3053
PsuI RGATCY 2 cut(s) 391, 3049
PsyI GACNNNGTC 2 cut(s) 7, 1642
PvuI CGATCG 1 cut(s) 650
PvuII CAGCTG 1 cut(s) 1967
RsaI GTAC 6 cut(s) 235, 1016, 1064, 1122, 3124, 3241
RsaNI GTAC 6 cut(s) 234, 1015, 1063, 1121, 3123, 3240
RseI CAYNNNNRTG 4 cut(s) 444, 1160, 3111, 4093
SacI GAGCTC 1 cut(s) 1221
SacII CCGCGG 1 cut(s) 905
SapI GCTCTTC 4 cut(s) 518, 2073, 4023, 4144
Sau96I GGNCC 8 cut(s) 156, 398, 720, 921, 1523, 3086, 3977, 4098
SduI GDGCHC 4 cut(s) 1169, 1221, 2464, 3387
SexAI ACCWGGT 2 cut(s) 1350, 3328
SfcI CTRYAG 3 cut(s) 3132, 3153, 3671
SfoI GGCGCC 1 cut(s) 1302
Sfr274I CTCGAG 1 cut(s) 2718
Sfr303I CCGCGG 1 cut(s) 905
SgrBI CCGCGG 1 cut(s) 905
SinI GGWCC 8 cut(s) 156, 398, 720, 921, 1523, 3086, 3977, 4098
SlaI CTCGAG 1 cut(s) 2718
SmaI CCCGGG 1 cut(s) 1339
SmiMI CAYNNNNRTG 4 cut(s) 444, 1160, 3111, 4093
SmlI CTYRAG 5 cut(s) 464, 1178, 1190, 2718, 3815
SmoI CTYRAG 5 cut(s) 464, 1178, 1190, 2718, 3815
SphI GCATGC 1 cut(s) 3914
SseBI AGGCCT 1 cut(s) 270
SspDI GGCGCC 1 cut(s) 1300
SspMI CTAG 8 cut(s) 249, 831, 1329, 1770, 2625, 2700, 2931, 3558
SstI GAGCTC 1 cut(s) 1221
StuI AGGCCT 1 cut(s) 270
StyI CCWWGG 3 cut(s) 2529, 4044, 4165
TaiI ACGT 6 cut(s) 197, 1403, 2417, 3808, 4093, 4214
TaqII GACCGA 1 cut(s) 386
TatI WGTACW 3 cut(s) 1014, 1062, 3122
TauI GCSGC 2 cut(s) 904, 1306
TscAI CASTG 7 cut(s) 88, 234, 1459, 1851, 2200, 3132, 4100
TseFI GTSAC 4 cut(s) 317, 2400, 2499, 2865
Tsp45I GTSAC 4 cut(s) 317, 2400, 2499, 2865
TspGWI ACGGA 8 cut(s) 122, 905, 1108, 2198, 3112, 3316, 3997, 4118
TspMI CCCGGG 1 cut(s) 1337
TspRI CASTG 7 cut(s) 88, 234, 1459, 1851, 2200, 3132, 4100
Tth111I GACNNNGTC 2 cut(s) 7, 1642
Van91I CCANNNNNTGG 1 cut(s) 19
Vha464I CTTAAG 1 cut(s) 1178
VneI GTGCAC 1 cut(s) 2460
VpaK11BI GGWCC 8 cut(s) 156, 398, 720, 921, 1523, 3086, 3977, 4098
XagI CCTNNNNNAGG 2 cut(s) 3189, 3671
XceI RCATGY 2 cut(s) 3903, 3914
XcmI CCANNNNNNNNNTGG 1 cut(s) 1138
XhoI CTCGAG 1 cut(s) 2718
XmaI CCCGGG 1 cut(s) 1337
XmiI GTMKAC 2 cut(s) 3376, 3541
XmnI GAANNNNTTC 5 cut(s) 1883, 2204, 2610, 3504, 3843
XspI CTAG 8 cut(s) 249, 831, 1329, 1770, 2625, 2700, 2931, 3558
ZraI GACGTC 3 cut(s) 195, 1401, 2415
Zsp2I ATGCAT 1 cut(s) 1543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.