RLG00000018575

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012264
Physical Location & Seq
Forward (+)
32103697 .. 32116910
13214 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018575

Sequence Viewer

Length: 2853 bp
ATGGGTGAGTGGGCGCCGCTGGTTCGGCCTCAGAGTGAATCTAGTAGCCCCGGGAGTAAGAACCAGGTGTTGAATAGACTGTTTTCATCTAAGCAGAAACTGTTGTCATTGACGTCTTCGCCTCCTTCTCAGAGGTCTTTTTCATTCTCGCCTTCATCGGGAAACAGTGTGCAAGAGGATGGTGGAGGGCAGTTGTCTCCTGATGAGAACAAGATTTTTAAGAAGTTCATTCCAGGTCCAAAGGTTAGGGATGCATTGCTGTTTAGGAAGTCGGTGGAGAAAGATGAGGATGACTCTGAGAAGGATGGGTTCTTTAAGAGGCTGCTAAGGGACAGTAGAGGTGATGATGAGACGGGGTCGAAGCGTAGAGATTCTTTGCTATTTAGGAAGTCATCGGAGAAGGATGAGGATGATACAGAAAAGGATGGGTTCTTTAAGAGATTATTAAGGGATAGTAAAGGGGACGATGAAGAATTAACTTCTAGTTCAGAGGGGTTTTTTAAGAAATTGTTTCGTGAAAGTAAGAGCGATTCCGAGGATAAATCAGTTTCTAAATCAGTGGACGATGAAGAAAAAGATGGGTTCTTTAAGAAGTTTTTCAAAGAGAAATTTGAGGACAAAAAGGATAGAAATGAAGATGATGATACAGCACACTCAGAAGGAAAATGCTCGAAATCAGCTGAAGATGATGAGAAAGATGGGTTTTTCCGAAAGTTATTTAGTAATAAGTTTGAGGACAGGAAAGATGGGAATGATAAAACTGAGGAAGGGAGTGCCAATGGGGAGGACGAAGAGCATTCTGACTTTTCATTATTCCGAAGATTGTTCCGCGTGCATCCTGAAGATGGCAAAAGTATGAATAATGAAAGCACCAATGGTAGCAGCTTGTTAGAAAGTAGTCCAGGCACTGAGAATTTTTTCCGTAAATTGTTTAGAGATCGAGACCGTTCAGTTGAAGATTCAGAGCTATTTGGTTCAAAAAAACATAAAGAGAAACATCCTGGTTCACCAAGGCAACAAAACGAAAAGTCAAGTGCAAAACCCCCACTTCCAATTAATTCAGCATCCCATTACCGTAAAGGGGCATACCATGAGTCATTGGAGTTTGTGCAGTCACTATGCGAGACATCATATGGTTTAGTGGATATATTTCCAATTGAAGATCGCAAAAGTGCTCTTCGTGAGTCACTCGCAGAGATCAATTTGCACATTGCCAAGGCGCAAAATAATGGAGGAGTTGGCTTTCCAATGGGGAAGGGGATGTATCGCGTGGTGTATATACCAGAAGATGAAGCTGTTCTTTTGAATTCTAGGGAGAAGGCACCCTACCTCATCTGTGTTGAAGTCTTAAAAAGTGAAATTCCAAGCAATCCAAAGGATACATCTGGCTCTCAAAAGCTCTCTCGAGGAGGAATTCCTTTAGCAAATGGAGATGCTCTTCTGCCAAGACCACCTCCCTGGGCATATCCTTTGTGGACTGTCCAAGAGGTCTATCGTAATAGTAATGATAGGATGTCAAGTTCTACTGCTCATGCAATTGACCAGGCAATGACACATACGCCAGAGGCAAAAAAGAAATTTGTTACTCTGAAAATATCTGTGGAGAAGAAGTTGGCAAGCCAGACTCTGAAAATAGAGAATATATCAAACAGTAGTAGAGGAGAGGCTCTTAATTGTGCATCAAAAGAAGCACGAGGGAGTGATTTGGAGTGGGTAAGGGTGGTGCTTACAGCAGATCCTGGTGTTAGAATGGAGGACATTGAGGATCAAGGACCATCTCGACGGAAGGAACACCGCCGTGTTCCAAGTACAGTGGCTATAGAAGAAGTAAAGGCTGCTGCAGCAAAAGGAGAAGCACCTCCTGGACTCCCTTTGAAAGGGGCTGGTCAGGATTCATCAGATGCACAGCCAACAGCTAATGGTGGTACTCCTAAGGCCAGCGATGCGTTGTCTGGTGAACTTTGGGAGATAAAGAAGGAGAGAATACGGAAGTCTTCAGTTTATGGAAAGTTACCTGGTTGGGACTTGCGCTCTGTCATTGTGAAGAGTGGTGATGATTGTAGACAGGAGCACCTTGCTGTGCAGCTTATTTCTCACTTCTATGATATATTCCAAGAAGCAGGGCTTCCCCTCTGGTTGCGACCTTATGAAGTCTTAGTTACTTCTTCTTACACAGCTCTCATTGAAACAATTCCAGATACGGCTTCACTTCATTCTATCAAAAGTAGACATCCCAACATCACTAGCTTGCGACACTTCTTTGCTGCTAAGTATCAAGAAAATTCTCCAACTTTTAAACTTGCTCAGAGAAATTTTGTTGAAAGTATGGCTGGATATTCCTTAGTGTGCTACCTTCTGCAGGTGAAAGATAGGCACAATGGAAACCTCTTAATGGATGAAGAAGGTCATATTATACATATTGATTTTGGCTTCATGCTCTCCAATTCACCTGGGGGAGTAAATTTTGAGAGTGCTCCATTCAAGTTGACACGTGAACTTCTTGAGGTTATGGACTCTGATGCTGAAGGAGTTCCAAGCGAGTTCTTCGATTACTTTAAAGTTTTGTGCATTCAAGGGTTCCTTACATGTCGGAAGCATGCAGAACGTATTATTCTTCTTGTAGAGATGTTGCAGGACTCTGGTTTCCCATGCTTTAAAGGTGGTCCACGGACAATACAGAATCTAAGGAAACGATTTCACCTGAGTTTGACAGAAGAGCAATGTGTGTCCTTGGTGCTTTCCCTAATCAGCAGCAGCTTGGATGCTTGGCGGACACGTCAAGCCACCTCGGTGAGTGTTGATCACAAGTGCATATGTGAGTGGGCTTTGGCTTCTGATTTTCCTCGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012506 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017137 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0030258 GO:0030659 GO:0031090 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0035618 GO:0035619 GO:0040007 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044706 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048015 GO:0048017 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051704 GO:0051716 GO:0052742 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0097708 GO:0099402 GO:0120025 GO:0120038 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

951

Amino Acids

106.73

Weight (kDa)

6.14

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3_PI4_kinase PF00454 679 - 886 1e-38 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09350 AT5G64070 AT5G64070
fragaria_vesca FvH4_1g24240
malus_domestica MD09G1261800.v1.1 MD17G1256600.v1.1
prunus_persica Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1
pyrus_communis pycom09g17610 pycom17g25880
rosa_chinensis RchiOBHm_Chr2g0120731
rosa_laevigata RLG00000018575
rosa_multiflora Rmu_sc0000319.1_g000011
rosa_roxburghii Rroxscaffold_2G00123200
rosa_rugosa Rorug02G0230500
rosa_samantha Rh2BG298500 Rh2CG276200
rosa_wichuraiana Rw2G023030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2350
AatII GACGTC 1 cut(s) 116
Acc36I ACCTGC 1 cut(s) 2350
AccB1I GGYRCC 2 cut(s) 13, 1321
AccI GTMKAC 2 cut(s) 2062, 2227
AccII CGCG 2 cut(s) 831, 1269
AciI CCGC 4 cut(s) 17, 829, 1795, 2770
AclWI GGATC 2 cut(s) 1730, 1773
AcsI RAATTY 9 cut(s) 608, 913, 1306, 1359, 1413, 1577, 2281, 2311, 2461
AcuI CTGAAG 4 cut(s) 702, 861, 1980, 2544
AcvI CACGTG 1 cut(s) 2492
AcyI GRCGYC 2 cut(s) 14, 113
AfaI GTAC 2 cut(s) 1810, 1927
AfiI CCNNNNNNNGG 6 cut(s) 158, 845, 1081, 1877, 2359, 2392
AflIII ACRYGT 3 cut(s) 2489, 2585, 2774
AjiI CACGTC 1 cut(s) 2777
AleI CACNNNNGTG 2 cut(s) 1797, 2789
AloI GAACNNNNNNTCC 2 cut(s) 1504, 1536
Alw21I GWGCWC 3 cut(s) 1177, 2073, 2476
Alw26I GTCTC 4 cut(s) 201, 344, 936, 1118
AlwI GGATC 2 cut(s) 1730, 1773
AlwNI CAGNNNCTG 4 cut(s) 100, 908, 1627, 1739
Ama87I CYCGRG 2 cut(s) 50, 1404
AoxI GGCC 2 cut(s) 26, 1935
ApeKI GCWGC 9 cut(s) 322, 882, 1835, 1838, 1841, 2083, 2264, 2751, 2754
ApoI RAATTY 9 cut(s) 608, 913, 1306, 1359, 1413, 1577, 2281, 2311, 2461
AseI ATTAAT 1 cut(s) 1056
Asp700I GAANNNNTTC 5 cut(s) 596, 917, 1296, 2190, 2529
AspLEI GCGC 3 cut(s) 16, 1222, 2031
AspS9I GGNCC 3 cut(s) 236, 1772, 2663
AsuC2I CCSGG 2 cut(s) 51, 52
AsuHPI GGTGA 9 cut(s) 17, 353, 999, 1967, 2063, 2374, 2439, 2690, 2803
AvaI CYCGRG 2 cut(s) 50, 1404
AvaII GGWCC 3 cut(s) 236, 1772, 2663
AxyI CCTNAGG 1 cut(s) 1932
BanI GGYRCC 2 cut(s) 13, 1321
BarI GAAGNNNNNNTAC 2 cut(s) 1310, 1342
BauI CACGAG 1 cut(s) 1692
BbrPI CACGTG 1 cut(s) 2492
BbsI GAAGAC 2 cut(s) 108, 1986
Bbv12I GWGCWC 3 cut(s) 1177, 2073, 2476
BbvI GCAGC 9 cut(s) 309, 894, 1822, 1825, 1853, 2095, 2251, 2763, 2766
BccI CCATC 8 cut(s) 173, 299, 419, 572, 692, 740, 839, 1783
BceAI ACGGC 2 cut(s) 1782, 2217
BciVI GTATCC 1 cut(s) 1372
BclI TGATCA 1 cut(s) 2800
BcnI CCSGG 2 cut(s) 51, 52
BcoDI GTCTC 4 cut(s) 201, 344, 936, 1118
BfaI CTAG 4 cut(s) 42, 483, 1311, 2244
BfmI CTRYAG 3 cut(s) 1818, 1839, 2357
BfoI RGCGCY 1 cut(s) 17
BfuAI ACCTGC 1 cut(s) 2350
BfuI GTATCC 1 cut(s) 1372
Bme18I GGWCC 3 cut(s) 236, 1772, 2663
BmeT110I CYCGRG 2 cut(s) 50, 1404
BmgBI CACGTC 1 cut(s) 2777
BmgT120I GGNCC 3 cut(s) 236, 1772, 2663
BmiI GGNNCC 3 cut(s) 15, 1323, 2579
BmsI GCATC 9 cut(s) 241, 844, 1073, 1423, 1688, 1891, 1933, 2509, 2752
BpiI GAAGAC 2 cut(s) 108, 1986
BplI GAGNNNNNCTC 2 cut(s) 278, 310
Bpu10I CCTNAGC 1 cut(s) 326
BpuEI CTTGAG 1 cut(s) 2522
BpuMI CCSGG 2 cut(s) 51, 52
BsaAI YACGTR 1 cut(s) 2492
BsaHI GRCGYC 2 cut(s) 14, 113
BsaI GGTCTC 1 cut(s) 936
BsaXI ACNNNNNCTCC 2 cut(s) 1595, 1625
Bsc4I CCNNNNNNNGG 6 cut(s) 158, 845, 1081, 1877, 2359, 2392
Bse21I CCTNAGG 1 cut(s) 1932
Bse3DI GCAATG 4 cut(s) 254, 1209, 1554, 2726
BseLI CCNNNNNNNGG 6 cut(s) 158, 845, 1081, 1877, 2359, 2392
BseMI GCAATG 4 cut(s) 254, 1209, 1554, 2726
BseMII CTCAG 8 cut(s) 44, 143, 288, 669, 753, 900, 2318, 2693
BseRI GAGGAG 3 cut(s) 1248, 1422, 1674
BseXI GCAGC 9 cut(s) 309, 894, 1822, 1825, 1853, 2095, 2251, 2763, 2766
BsgI GTGCAG 2 cut(s) 1130, 2102
Bsh1236I CGCG 2 cut(s) 831, 1269
BshFI GGCC 2 cut(s) 28, 1937
BshNI GGYRCC 2 cut(s) 13, 1321
BsiHKAI GWGCWC 3 cut(s) 1177, 2073, 2476
BsiHKCI CYCGRG 2 cut(s) 50, 1404
BsiSI CCGG 1 cut(s) 51
BslFI GGGAC 3 cut(s) 344, 476, 2036
BslI CCNNNNNNNGG 6 cut(s) 158, 845, 1081, 1877, 2359, 2392
BsmAI GTCTC 4 cut(s) 201, 344, 936, 1118
BsmBI CGTCTC 1 cut(s) 344
BsmFI GGGAC 3 cut(s) 344, 476, 2036
BsmI GAATGC 2 cut(s) 796, 2568
BsnI GGCC 2 cut(s) 28, 1937
Bso31I GGTCTC 1 cut(s) 936
BsoBI CYCGRG 2 cut(s) 50, 1404
Bsp1286I GDGCHC 3 cut(s) 1177, 2073, 2476
Bsp143I GATC 6 cut(s) 937, 1162, 1197, 1735, 1765, 2800
BspACI CCGC 4 cut(s) 17, 829, 1795, 2770
BspANI GGCC 2 cut(s) 28, 1937
BspCNI CTCAG 8 cut(s) 43, 142, 289, 668, 754, 901, 2317, 2694
BspFNI CGCG 2 cut(s) 831, 1269
BspLI GGNNCC 3 cut(s) 15, 1323, 2579
BspMAI CTGCAG 2 cut(s) 1843, 2361
BspMI ACCTGC 1 cut(s) 2350
BspPI GGATC 2 cut(s) 1730, 1773
BspQI GCTCTTC 4 cut(s) 786, 1182, 1443, 2709
BspT107I GGYRCC 2 cut(s) 13, 1321
BspTNI GGTCTC 1 cut(s) 936
BsrDI GCAATG 4 cut(s) 254, 1209, 1554, 2726
BssMI GATC 6 cut(s) 937, 1162, 1197, 1735, 1765, 2800
BssNI GRCGYC 2 cut(s) 14, 113
BssSI CACGAG 1 cut(s) 1692
BssT1I CCWWGG 3 cut(s) 1010, 1215, 2730
Bst2BI CACGAG 1 cut(s) 1692
Bst4CI ACNGT 9 cut(s) 81, 102, 167, 335, 947, 1076, 1480, 1652, 1813
Bst6I CTCTTC 5 cut(s) 786, 1182, 1443, 2039, 2709
BstACI GRCGYC 2 cut(s) 14, 113
BstBAI YACGTR 1 cut(s) 2492
BstC8I GCNNGC 5 cut(s) 833, 1618, 1939, 2249, 2598
BstDSI CCRYGG 1 cut(s) 2666
BstENI CCTNNNNNAGG 2 cut(s) 1875, 2357
BstFNI CGCG 2 cut(s) 831, 1269
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 3 cut(s) 16, 1222, 2031
BstKTI GATC 6 cut(s) 940, 1165, 1200, 1738, 1768, 2803
BstMAI GTCTC 4 cut(s) 201, 344, 936, 1118
BstMBI GATC 6 cut(s) 937, 1162, 1197, 1735, 1765, 2800
BstMWI GCNNNNNNNGC 3 cut(s) 25, 1841, 1943
BstNSI RCATGY 2 cut(s) 2589, 2600
BstSFI CTRYAG 3 cut(s) 1818, 1839, 2357
BstUI CGCG 2 cut(s) 831, 1269
BstV1I GCAGC 9 cut(s) 309, 894, 1822, 1825, 1853, 2095, 2251, 2763, 2766
BstV2I GAAGAC 2 cut(s) 108, 1986
BstX2I RGATCY 1 cut(s) 1735
BstXI CCANNNNNNTGG 1 cut(s) 1458
BstYI RGATCY 1 cut(s) 1735
Bsu36I CCTNAGG 1 cut(s) 1932
BsuI GTATCC 1 cut(s) 1372
BsuRI GGCC 2 cut(s) 28, 1937
BtgI CCRYGG 1 cut(s) 2666
BtgZI GCGATG 1 cut(s) 1956
BtrI CACGTC 1 cut(s) 2777
BtsIMutI CAGTG 4 cut(s) 172, 564, 906, 1818
BveI ACCTGC 1 cut(s) 2350
Cac8I GCNNGC 5 cut(s) 833, 1618, 1939, 2249, 2598
CaiI CAGNNNCTG 4 cut(s) 100, 908, 1627, 1739
CfoI GCGC 3 cut(s) 16, 1222, 2031
Cfr13I GGNCC 3 cut(s) 236, 1772, 2663
Cfr9I CCCGGG 1 cut(s) 50
CsiI ACCWGGT 2 cut(s) 63, 2014
Csp6I GTAC 2 cut(s) 1809, 1926
CspCI CAANNNNNGTGG 2 cut(s) 1794, 1829
CviAII CATG 6 cut(s) 1091, 1532, 2434, 2586, 2597, 2649
CviQI GTAC 2 cut(s) 1809, 1926
DinI GGCGCC 1 cut(s) 15
DpnI GATC 6 cut(s) 939, 1164, 1199, 1737, 1767, 2802
DpnII GATC 6 cut(s) 937, 1162, 1197, 1735, 1765, 2800
DraI TTTAAA 3 cut(s) 2296, 2557, 2656
Eam1104I CTCTTC 5 cut(s) 786, 1182, 1443, 2039, 2709
EarI CTCTTC 5 cut(s) 786, 1182, 1443, 2039, 2709
EciI GGCGGA 1 cut(s) 2785
Eco130I CCWWGG 3 cut(s) 1010, 1215, 2730
Eco31I GGTCTC 1 cut(s) 936
Eco47I GGWCC 3 cut(s) 236, 1772, 2663
Eco57I CTGAAG 4 cut(s) 702, 861, 1980, 2544
Eco72I CACGTG 1 cut(s) 2492
Eco81I CCTNAGG 1 cut(s) 1932
Eco88I CYCGRG 2 cut(s) 50, 1404
EcoNI CCTNNNNNAGG 2 cut(s) 1875, 2357
EcoRI GAATTC 2 cut(s) 1306, 1413
EcoT14I CCWWGG 3 cut(s) 1010, 1215, 2730
EcoT22I ATGCAT 1 cut(s) 256
EgeI GGCGCC 1 cut(s) 15
EheI GGCGCC 1 cut(s) 15
ErhI CCWWGG 3 cut(s) 1010, 1215, 2730
Esp3I CGTCTC 1 cut(s) 344
FaeI CATG 6 cut(s) 1094, 1535, 2437, 2589, 2600, 2652
FalI AAGNNNNNCTT 6 cut(s) 1284, 1316, 2109, 2141, 2565, 2597
FaqI GGGAC 3 cut(s) 344, 476, 2036
FatI CATG 6 cut(s) 1090, 1531, 2433, 2585, 2596, 2648
FauNDI CATATG 2 cut(s) 1132, 2813
FbaI TGATCA 1 cut(s) 2800
FblI GTMKAC 2 cut(s) 2062, 2227
FspBI CTAG 4 cut(s) 42, 483, 1311, 2244
GlaI GCGC 3 cut(s) 15, 1221, 2030
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 2 cut(s) 28, 1937
HapII CCGG 1 cut(s) 51
HhaI GCGC 3 cut(s) 16, 1222, 2031
Hin1I GRCGYC 2 cut(s) 14, 113
Hin1II CATG 6 cut(s) 1094, 1535, 2437, 2589, 2600, 2652
Hin6I GCGC 3 cut(s) 14, 1220, 2029
HinP1I GCGC 3 cut(s) 14, 1220, 2029
HincII GTYRAC 1 cut(s) 2487
HindII GTYRAC 1 cut(s) 2487
HpaII CCGG 1 cut(s) 51
HphI GGTGA 9 cut(s) 17, 353, 999, 1967, 2063, 2374, 2439, 2690, 2803
Hpy166II GTNNAC 9 cut(s) 562, 1007, 1476, 1958, 2063, 2228, 2487, 2495, 2666
Hpy8I GTNNAC 9 cut(s) 562, 1007, 1476, 1958, 2063, 2228, 2487, 2495, 2666
Hpy99I CGWCG 1 cut(s) 1785
HpyCH4III ACNGT 9 cut(s) 81, 102, 167, 335, 947, 1076, 1480, 1652, 1813
HpyCH4IV ACGT 4 cut(s) 113, 2491, 2605, 2776
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 1841, 1943
HpySE526I ACGT 4 cut(s) 113, 2491, 2605, 2776
Hsp92I GRCGYC 2 cut(s) 14, 113
Hsp92II CATG 6 cut(s) 1094, 1535, 2437, 2589, 2600, 2652
HspAI GCGC 3 cut(s) 14, 1220, 2029
KasI GGCGCC 1 cut(s) 13
Ksp22I TGATCA 1 cut(s) 2800
Kzo9I GATC 6 cut(s) 937, 1162, 1197, 1735, 1765, 2800
LguI GCTCTTC 4 cut(s) 786, 1182, 1443, 2709
LmnI GCTCC 2 cut(s) 2068, 2479
Lsp1109I GCAGC 9 cut(s) 309, 894, 1822, 1825, 1853, 2095, 2251, 2763, 2766
LweI GCATC 9 cut(s) 241, 844, 1073, 1423, 1688, 1891, 1933, 2509, 2752
MabI ACCWGGT 2 cut(s) 63, 2014
MaeI CTAG 4 cut(s) 42, 483, 1311, 2244
MaeII ACGT 4 cut(s) 113, 2491, 2605, 2776
MaeIII GTNAC 5 cut(s) 1113, 1185, 1582, 2010, 2158
MalI GATC 6 cut(s) 939, 1164, 1199, 1737, 1767, 2802
MboI GATC 6 cut(s) 937, 1162, 1197, 1735, 1765, 2800
MfeI CAATTG 2 cut(s) 1155, 1536
MflI RGATCY 1 cut(s) 1735
MhlI GDGCHC 3 cut(s) 1177, 2073, 2476
Mly113I GGCGCC 1 cut(s) 14
MlyI GAGTC 7 cut(s) 287, 1103, 1193, 1618, 1860, 2507, 2630
MmeI TCCRAC 2 cut(s) 2312, 2570
Mph1103I ATGCAT 1 cut(s) 256
MroXI GAANNNNTTC 5 cut(s) 596, 917, 1296, 2190, 2529
MslI CAYNNNNRTG 3 cut(s) 1797, 2100, 2789
MspA1I CMGCKG 2 cut(s) 19, 680
MspI CCGG 1 cut(s) 51
MunI CAATTG 2 cut(s) 1155, 1536
Mva1269I GAATGC 2 cut(s) 796, 2568
MvnI CGCG 2 cut(s) 831, 1269
MwoI GCNNNNNNNGC 3 cut(s) 25, 1841, 1943
NarI GGCGCC 1 cut(s) 14
NciI CCSGG 2 cut(s) 51, 52
NdeI CATATG 2 cut(s) 1132, 2813
NdeII GATC 6 cut(s) 937, 1162, 1197, 1735, 1765, 2800
NlaIII CATG 6 cut(s) 1094, 1535, 2437, 2589, 2600, 2652
NlaIV GGNNCC 3 cut(s) 15, 1323, 2579
NmuCI GTSAC 2 cut(s) 1113, 1185
NsiI ATGCAT 1 cut(s) 256
NspI RCATGY 2 cut(s) 2589, 2600
OliI CACNNNNGTG 2 cut(s) 1797, 2789
PaeI GCATGC 1 cut(s) 2600
PaeR7I CTCGAG 1 cut(s) 1404
PaqCI CACCTGC 1 cut(s) 2350
PasI CCCWGGG 1 cut(s) 1458
PciI ACATGT 1 cut(s) 2585
PciSI GCTCTTC 4 cut(s) 786, 1182, 1443, 2709
PctI GAATGC 2 cut(s) 796, 2568
PdmI GAANNNNTTC 5 cut(s) 596, 917, 1296, 2190, 2529
PfeI GAWTC 6 cut(s) 38, 371, 530, 959, 1892, 2680
PflFI GACNNNGTC 1 cut(s) 355
PfoI TCCNGGA 1 cut(s) 1861
PleI GAGTC 7 cut(s) 287, 1102, 1192, 1618, 1860, 2507, 2630
PluTI GGCGCC 1 cut(s) 17
PmaCI CACGTG 1 cut(s) 2492
PmlI CACGTG 1 cut(s) 2492
PpsI GAGTC 7 cut(s) 287, 1102, 1192, 1618, 1860, 2507, 2630
Ppu21I YACGTR 1 cut(s) 2492
PscI ACATGT 1 cut(s) 2585
PshBI ATTAAT 1 cut(s) 1056
PspCI CACGTG 1 cut(s) 2492
PspN4I GGNNCC 3 cut(s) 15, 1323, 2579
PspPI GGNCC 3 cut(s) 236, 1772, 2663
PstI CTGCAG 2 cut(s) 1843, 2361
PstNI CAGNNNCTG 4 cut(s) 100, 908, 1627, 1739
PsuI RGATCY 1 cut(s) 1735
PsyI GACNNNGTC 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 680
RsaI GTAC 2 cut(s) 1810, 1927
RsaNI GTAC 2 cut(s) 1809, 1926
RseI CAYNNNNRTG 3 cut(s) 1797, 2100, 2789
SapI GCTCTTC 4 cut(s) 786, 1182, 1443, 2709
Sau3AI GATC 6 cut(s) 937, 1162, 1197, 1735, 1765, 2800
Sau96I GGNCC 3 cut(s) 236, 1772, 2663
SchI GAGTC 7 cut(s) 287, 1103, 1193, 1618, 1860, 2507, 2630
SduI GDGCHC 3 cut(s) 1177, 2073, 2476
SexAI ACCWGGT 2 cut(s) 63, 2014
SfaNI GCATC 9 cut(s) 241, 844, 1073, 1423, 1688, 1891, 1933, 2509, 2752
SfcI CTRYAG 3 cut(s) 1818, 1839, 2357
SfoI GGCGCC 1 cut(s) 15
Sfr274I CTCGAG 1 cut(s) 1404
SinI GGWCC 3 cut(s) 236, 1772, 2663
SlaI CTCGAG 1 cut(s) 1404
SmaI CCCGGG 1 cut(s) 52
SmiMI CAYNNNNRTG 3 cut(s) 1797, 2100, 2789
SmlI CTYRAG 2 cut(s) 1404, 2501
SmoI CTYRAG 2 cut(s) 1404, 2501
SphI GCATGC 1 cut(s) 2600
SsiI CCGC 4 cut(s) 17, 829, 1795, 2770
SspDI GGCGCC 1 cut(s) 13
SspMI CTAG 4 cut(s) 42, 483, 1311, 2244
StyI CCWWGG 3 cut(s) 1010, 1215, 2730
TaaI ACNGT 9 cut(s) 81, 102, 167, 335, 947, 1076, 1480, 1652, 1813
TaiI ACGT 4 cut(s) 116, 2494, 2608, 2779
TaqI TCGA 6 cut(s) 359, 671, 940, 1405, 1780, 2547
TatI WGTACW 1 cut(s) 1808
TauI GCSGC 1 cut(s) 19
TfiI GAWTC 6 cut(s) 38, 371, 530, 959, 1892, 2680
TscAI CASTG 4 cut(s) 172, 564, 913, 1818
TseFI GTSAC 2 cut(s) 1113, 1185
TseI GCWGC 9 cut(s) 322, 882, 1835, 1838, 1841, 2083, 2264, 2751, 2754
Tsp45I GTSAC 2 cut(s) 1113, 1185
TspGWI ACGGA 4 cut(s) 911, 1798, 2002, 2683
TspMI CCCGGG 1 cut(s) 50
TspRI CASTG 4 cut(s) 172, 564, 913, 1818
Tth111I GACNNNGTC 1 cut(s) 355
VpaK11BI GGWCC 3 cut(s) 236, 1772, 2663
VspI ATTAAT 1 cut(s) 1056
XagI CCTNNNNNAGG 2 cut(s) 1875, 2357
XapI RAATTY 9 cut(s) 608, 913, 1306, 1359, 1413, 1577, 2281, 2311, 2461
XceI RCATGY 2 cut(s) 2589, 2600
XhoI CTCGAG 1 cut(s) 1404
XmaI CCCGGG 1 cut(s) 50
XmiI GTMKAC 2 cut(s) 2062, 2227
XmnI GAANNNNTTC 5 cut(s) 596, 917, 1296, 2190, 2529
XspI CTAG 4 cut(s) 42, 483, 1311, 2244
ZraI GACGTC 1 cut(s) 114
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.