FvH4_1g24240

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012264
Physical Location & Seq
Forward (+)
16112824 .. 16128074
15251 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24240.t1

Sequence Viewer

Length: 3270 bp
ATGGTGAGGATACTCGGACTGAGTCGCGGCGAATCGGAAGAGTCGCCGCGTGAGATCACCTCCCGGACGCCGACGACTCTTTCAAGTGACTCCGGCGAGAACGGGTGGCTGATTCGCTTCTTCGACTCGGCTTTCTTCTGTGAGTGGATCGCAGTGAGCTACCTGTACAAACATGAACACGCCGGGGTTCGAGACTATCTCTGTAACCGAATGTACACGCTGCCATTGCCAGGTATTGAGAGCTATTTGTTTCAGATATGTTATATGTCTGTACATAAACCCAGCCCTTCTTTGGATAAGTTTGTTATTGATATGTGTTCAAAGTCGCTTAAGATGGCTCTCAAGGTGCATTGGTTTCTATTGGCTGAGCTCGAAGATTCCGATGACAATGAGGGGATTAGTAGGATACAGGAGAAGTGCCAGATTGCTGCTACTTTGATGGGGGAGTGGGCCCCGCTGGTTCGGCCCCAGAGTGAGTCTGGGAGCAGCCCCGGGAGTAAGAACCAGGTGTTGAATAGACTGTTTTCGTCTAAGCAGAAGCTCTTGTCATTGACTTCTTCACCACCGGCTCAGAGGTCCTTTTCGTTCTCCCCGGGGAACAGTGTAGGGCAGGAGGATGGTGGTGGGCAGTTGTCTCCTGATGAGAATAAGATTTTTAAGAAGTTTATTCCGGGGCCCAAGGTTAGGGATGCTTTGCTGTTTAGGAAGTCGGCGGAGAAAGAGAAGGATGAGGATGAGTCTGAGAAGGATGGGTTCTTTAAGAGGCTACTGAGGGACAGTAGAGGTGATGATGAGACTCCCTCGAAGATTAGAGATTCTTTGCTGTTTAGGAAGTCATCGGAGAAGGATGATGATGATACAGAAAAGGATGGGTTCTTTAAGAGATTATTAAGGGATAGTAAAGGGGACGATGAAGAATTAACTTCTAGTTCAGAGGGATTTTTTAAGAGATTGTTTCGTGAAAGTAAGAGTGAATCCGAGGATAAATCTGTTTCTAAATCTGTGGAGGATGACGAAAAAGATGGATTCTTTAAGAAGTTTTTCAAAGAGAAATTTGAGGACAAAAAAGATAGAATTGATAGGAATGAAGATGAGGATACAGCACACTCAGAAGGAAGATGCTCGAAATCAGCTGAAGATGATGAGAAAGATGGGTTCTTCCGGAAATTGTTTAGTAATAAGTTTGAGGACAGGAAAGATGGGAATGATAAAACTGAGGAAGGGAGTGTTAATGGGGAGGATGAAGAGCCTTCTGACTTTTCATTATTCCGAAGATTGTTCCGCGTGCACCCTGAAGATGGCAAAAGTACTGCTATTGAAAGCATCAATGGTGGCAGCTTGCTAGAAAGTAGTCCAGGGACTGAGAGTTTTTTCCGTAAATTGTTTAGAGATCGAGACCGTTCGGTTGAAGATTCAGAGCTATTTGGTTCAAAAAAACATAAAGATAAACGTCCTGGTTCGCCAAAGCAACAAAACGAAAAGTCAAGTGTAAAACCCCCACTTCCAAGTTCAGCATCCCATTACCGTAAAGGCGCATACCATGAGTCATTGGAGTTTGTGCAGTCACTATGCGAGACGTCATATGGTTTAGTGGATATATTTCCAATTGAAGATCGCAAAAGTGCTCTTCGTGAGTCACTCGCAGAGATCAATTTGCACATTGCTGAGTCGCAAAATAATGGAGGAGTTGGCTTTCCAATGGGGAAGGGGATGTATCGCGTGGTGTATATACCAGAAGATGAAGCTGTTCTTCTGAATTCTAGGGAGAAGGCACCCTACCTCATCTGTGTTGAAGTCTTGAAAAGTGAAATTTCTAGCAATCCAAAGGATACATCAGGCTCTCAAAAGCTCTCTCGAGGAGGAATTCCTTTAGCAACTGGAGATGCACTTCTGCCAAGACCACCTCCCTGGGCATATCCTTTGTGGACTGTCCAAGAGGTCTATCGTAATAGTAATGATAGGATGTCAAGTTCTACTGCTCATGCAATTGACCAGGCAATGTCACATACTGAGCAAAAAGTGAAATTTGTTACTTTGAAAATATCTGTTGGGAAGAAGTTGCCTAGCCAGACTCTGAAAACAGAGAATAGATCACACAGTTGTAAAGGAGGAGAGGTTCTTGGATGTGCATCAAAAGAAGCACAAATGAGTGATTTGGAGTGGGTAAGGGTGGTGCTCACAGCAGATCCTGGTGTTAGAATGGAGGACATTGAGGATCAAGGACCGGTTCGACGGAAGGAACACCGTCGTGTTCCAAGTACAGTGGCTATAGAAGAAGTAAAGGCTGCTGCAGCGAAAGGAGAAGCACCTCCTGGACTCCCTTTGAAAGGGGCTGGTCAGGATTCATCAGATGCACAGCCAACGGCTAATGGTTGTACTCCTAAGGCCAGCGATGCGTTGTCTGGTGAACTTTGGGAGATGAAGAGGGAGAGAATACGCAAGTCATCAGTTTATGGAAAGTTACCTGGTTGGGACTTGCGCTCTATCATTGTGAAGAGTGGTGATGATTGTAGACAGGAGCATCTTGCTGTGCAGCTTATTTCTCACTTCTATGATATATTCCAAGAAGCTGGGCTTCCCCTCTGGTTGCGACCTTATGAAGTCTTAGTTACTTCTTCTTACACAGCTCTCATTGAAACAATTCCAGATACGGCTTCACTTCATTCTATCAAAAGTAGATATCCTAACATCACTAGCTTGCGACACTTCTTTGCTGCTAAGTATCAAGAAAATTCTCCAACTTTTAAACTTGCCCAGAGAAACTTTGTTGAAAGTATGGCTGGATATTCCTTAGTGTGCTACCTTCTGCAGGTGAAGGATAGGCACAATGGAAACCTCTTAATGGACGAAGAAGGTCATATTATACATATTGATTTTGGCTTCATGCTCTCCAATTCACCTGGGGGTGTAAATTTTGAGAGTGCTCCATTCAAGTTGACACGTGAACTTCTTGAGGTTATGGATTCTGATGCCGAAGGAGTTCCGAGCGAGTTCTTTGATTACTTTAAAGTTTTGTGCATTCAAGGGTTCCTTACATGTCGGAAGCATGCAGAACGAATTATTCTTCTTGTAGAGATGTTGCAGGACTCTGGTTTCCCATGCTTTAAAGGTGGTCAACGGACAATACAGAATCTAAGGAAACGATTTCACCTGAGTTTGACAGAAGAGCAATGTGTGTCCTTGGTGCTTTCCTTAATCAGCAACAGCTTGGATGCTTGGCGGACACGGCAGTATGATTATTATCAGAGAGTACTGAATGGAATATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012506 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017137 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0030258 GO:0030659 GO:0031090 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0035618 GO:0035619 GO:0040007 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044706 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048015 GO:0048017 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051704 GO:0051716 GO:0052742 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0097708 GO:0099402 GO:0120025 GO:0120038 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

1090

Amino Acids

122.92

Weight (kDa)

5.85

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI4KB-PIK1_PIK PF21245 50 - 120 9.4e-12 PI4KB/PIK1, accessory (PIK) domain
PI3_PI4_kinase PF00454 829 - 1036 8.5e-39 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09350 AT5G64070 AT5G64070
fragaria_vesca FvH4_1g24240
malus_domestica MD09G1261800.v1.1 MD17G1256600.v1.1
prunus_persica Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1
pyrus_communis pycom09g17610 pycom17g25880
rosa_chinensis RchiOBHm_Chr2g0120731
rosa_laevigata RLG00000018575
rosa_multiflora Rmu_sc0000319.1_g000011
rosa_roxburghii Rroxscaffold_2G00123200
rosa_rugosa Rorug02G0230500
rosa_samantha Rh2BG298500 Rh2CG276200
rosa_wichuraiana Rw2G023030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2800
AatII GACGTC 1 cut(s) 1580
Acc36I ACCTGC 1 cut(s) 2800
AccB1I GGYRCC 1 cut(s) 1771
AccI GTMKAC 1 cut(s) 2512
AccII CGCG 4 cut(s) 27, 49, 1284, 1719
AccIII TCCGGA 1 cut(s) 1161
AciI CCGC 6 cut(s) 27, 47, 455, 713, 1282, 3220
AclWI GGATC 3 cut(s) 155, 2180, 2223
AcsI RAATTY 7 cut(s) 1052, 1756, 1809, 1863, 2024, 2731, 2911
AcuI CTGAAG 2 cut(s) 1155, 1314
AcvI CACGTG 1 cut(s) 2942
AcyI GRCGYC 2 cut(s) 68, 1577
AfaI GTAC 7 cut(s) 167, 215, 273, 1309, 2260, 2377, 3252
AfiI CCNNNNNNNGG 7 cut(s) 230, 292, 684, 1298, 2327, 2809, 2842
AflII CTTAAG 1 cut(s) 329
AflIII ACRYGT 2 cut(s) 2939, 3035
AgeI ACCGGT 1 cut(s) 2224
AleI CACNNNNGTG 1 cut(s) 2247
AloI GAACNNNNNNTCC 4 cut(s) 1954, 1986, 2100, 2132
Alw21I GWGCWC 5 cut(s) 372, 1290, 1627, 2178, 2926
Alw26I GTCTC 5 cut(s) 186, 639, 788, 1389, 1568
Alw44I GTGCAC 1 cut(s) 1286
AlwI GGATC 3 cut(s) 155, 2180, 2223
AlwNI CAGNNNCTG 3 cut(s) 1361, 2074, 2189
Ama87I CYCGRG 3 cut(s) 491, 592, 1854
Aor13HI TCCGGA 1 cut(s) 1161
AoxI GGCC 4 cut(s) 450, 464, 674, 2385
ApaI GGGCCC 2 cut(s) 454, 678
ApaLI GTGCAC 1 cut(s) 1286
ApeKI GCWGC 9 cut(s) 220, 428, 486, 1335, 2285, 2288, 2291, 2533, 2714
ApoI RAATTY 7 cut(s) 1052, 1756, 1809, 1863, 2024, 2731, 2911
AsiGI ACCGGT 1 cut(s) 2224
Asp700I GAANNNNTTC 4 cut(s) 1040, 1746, 2640, 2979
AspLEI GCGC 2 cut(s) 1535, 2481
AspS9I GGNCC 7 cut(s) 450, 451, 465, 576, 674, 675, 2222
AsuC2I CCSGG 7 cut(s) 64, 184, 492, 493, 593, 594, 672
AsuHPI GGTGA 9 cut(s) 16, 49, 552, 797, 2417, 2513, 2824, 2889, 3140
AvaI CYCGRG 3 cut(s) 491, 592, 1854
AvaII GGWCC 2 cut(s) 576, 2222
AxyI CCTNAGG 1 cut(s) 2382
BaeGI GKGCMC 3 cut(s) 454, 678, 1290
BanI GGYRCC 1 cut(s) 1771
BanII GRGCYC 3 cut(s) 372, 454, 678
BarI GAAGNNNNNNTAC 2 cut(s) 1760, 1792
BbrPI CACGTG 1 cut(s) 2942
Bbv12I GWGCWC 5 cut(s) 372, 1290, 1627, 2178, 2926
BbvI GCAGC 9 cut(s) 207, 415, 498, 1347, 2272, 2275, 2303, 2545, 2701
BccI CCATC 9 cut(s) 328, 433, 611, 743, 863, 1016, 1145, 1193, 1292
BceAI ACGGC 3 cut(s) 2379, 2667, 3242
BciVI GTATCC 4 cut(s) 3, 399, 1090, 1822
BcnI CCSGG 7 cut(s) 64, 184, 492, 493, 593, 594, 672
BcoDI GTCTC 5 cut(s) 186, 639, 788, 1389, 1568
BfaI CTAG 6 cut(s) 927, 1343, 1761, 1815, 2064, 2694
BfmI CTRYAG 3 cut(s) 2268, 2289, 2807
BfrI CTTAAG 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 2800
BfuI GTATCC 4 cut(s) 3, 399, 1090, 1822
BlpI GCTNAGC 1 cut(s) 366
BmcAI AGTACT 2 cut(s) 1309, 3252
Bme18I GGWCC 2 cut(s) 576, 2222
BmeT110I CYCGRG 3 cut(s) 491, 592, 1854
BmgT120I GGNCC 7 cut(s) 450, 451, 465, 576, 674, 675, 2222
BmiI GGNNCC 7 cut(s) 452, 453, 467, 675, 676, 1773, 3029
BplI GAGNNNNNCTC 6 cut(s) 44, 76, 183, 215, 785, 817
BpmI CTGGAG 1 cut(s) 1899
Bpu1102I GCTNAGC 1 cut(s) 366
BpuEI CTTGAG 2 cut(s) 326, 2972
BpuMI CCSGG 7 cut(s) 64, 184, 492, 493, 593, 594, 672
BsaAI YACGTR 1 cut(s) 2942
BsaBI GATNNNNATC 2 cut(s) 2128, 3240
BsaHI GRCGYC 2 cut(s) 68, 1577
BsaI GGTCTC 1 cut(s) 1389
BsaWI WCCGGW 2 cut(s) 1161, 2224
BsaXI ACNNNNNCTCC 2 cut(s) 2100, 2130
Bsc4I CCNNNNNNNGG 7 cut(s) 230, 292, 684, 1298, 2327, 2809, 2842
Bse118I RCCGGY 2 cut(s) 565, 2224
Bse1I ACTGG 1 cut(s) 1882
Bse21I CCTNAGG 1 cut(s) 2382
Bse3DI GCAATG 4 cut(s) 224, 1659, 2004, 3176
Bse8I GATNNNNATC 2 cut(s) 2128, 3240
BseAI TCCGGA 1 cut(s) 1161
BseJI GATNNNNATC 2 cut(s) 2128, 3240
BseLI CCNNNNNNNGG 7 cut(s) 230, 292, 684, 1298, 2327, 2809, 2842
BseMI GCAATG 4 cut(s) 224, 1659, 2004, 3176
BseNI ACTGG 1 cut(s) 1882
BseRI GAGGAG 3 cut(s) 1698, 1872, 2124
BseSI GKGCMC 3 cut(s) 454, 678, 1290
BseXI GCAGC 9 cut(s) 207, 415, 498, 1347, 2272, 2275, 2303, 2545, 2701
BseYI CCCAGC 2 cut(s) 281, 2570
BsgI GTGCAG 2 cut(s) 1580, 2552
Bsh1236I CGCG 4 cut(s) 27, 49, 1284, 1719
BshFI GGCC 4 cut(s) 452, 466, 676, 2387
BshNI GGYRCC 1 cut(s) 1771
BshTI ACCGGT 1 cut(s) 2224
BsiHKAI GWGCWC 5 cut(s) 372, 1290, 1627, 2178, 2926
BsiHKCI CYCGRG 3 cut(s) 491, 592, 1854
BsiSI CCGG 9 cut(s) 64, 93, 183, 492, 566, 593, 671, 1162, 2225
BslFI GGGAC 4 cut(s) 788, 920, 1372, 2486
BslI CCNNNNNNNGG 7 cut(s) 230, 292, 684, 1298, 2327, 2809, 2842
BsmAI GTCTC 5 cut(s) 186, 639, 788, 1389, 1568
BsmBI CGTCTC 1 cut(s) 1568
BsmFI GGGAC 4 cut(s) 788, 920, 1372, 2486
BsmI GAATGC 1 cut(s) 3018
BsnI GGCC 4 cut(s) 452, 466, 676, 2387
Bso31I GGTCTC 1 cut(s) 1389
BsoBI CYCGRG 3 cut(s) 491, 592, 1854
Bsp120I GGGCCC 2 cut(s) 450, 674
Bsp1286I GDGCHC 7 cut(s) 372, 454, 678, 1290, 1627, 2178, 2926
Bsp13I TCCGGA 1 cut(s) 1161
Bsp1407I TGTACA 3 cut(s) 165, 213, 271
Bsp143I GATC 8 cut(s) 54, 147, 1390, 1612, 1647, 2090, 2185, 2215
Bsp1720I GCTNAGC 1 cut(s) 366
BspACI CCGC 6 cut(s) 27, 47, 455, 713, 1282, 3220
BspANI GGCC 4 cut(s) 452, 466, 676, 2387
BspEI TCCGGA 1 cut(s) 1161
BspFNI CGCG 4 cut(s) 27, 49, 1284, 1719
BspLI GGNNCC 7 cut(s) 452, 453, 467, 675, 676, 1773, 3029
BspMAI CTGCAG 2 cut(s) 2293, 2811
BspMI ACCTGC 1 cut(s) 2800
BspPI GGATC 3 cut(s) 155, 2180, 2223
BspQI GCTCTTC 3 cut(s) 1239, 1632, 3159
BspT107I GGYRCC 1 cut(s) 1771
BspTI CTTAAG 1 cut(s) 329
BspTNI GGTCTC 1 cut(s) 1389
BsrDI GCAATG 4 cut(s) 224, 1659, 2004, 3176
BsrFI RCCGGY 2 cut(s) 565, 2224
BsrGI TGTACA 3 cut(s) 165, 213, 271
BsrI ACTGG 1 cut(s) 1882
BssAI RCCGGY 2 cut(s) 565, 2224
BssMI GATC 8 cut(s) 54, 147, 1390, 1612, 1647, 2090, 2185, 2215
BssNI GRCGYC 2 cut(s) 68, 1577
BssT1I CCWWGG 2 cut(s) 678, 3180
Bst4CI ACNGT 9 cut(s) 522, 602, 779, 1400, 1526, 1930, 2099, 2246, 2263
Bst6I CTCTTC 6 cut(s) 33, 1239, 1632, 2417, 2489, 3159
BstACI GRCGYC 2 cut(s) 68, 1577
BstAFI CTTAAG 1 cut(s) 329
BstAUI TGTACA 3 cut(s) 165, 213, 271
BstBAI YACGTR 1 cut(s) 2942
BstC8I GCNNGC 5 cut(s) 1286, 1340, 2389, 2699, 3048
BstENI CCTNNNNNAGG 2 cut(s) 2325, 2807
BstFNI CGCG 4 cut(s) 27, 49, 1284, 1719
BstHHI GCGC 2 cut(s) 1535, 2481
BstKTI GATC 8 cut(s) 57, 150, 1393, 1615, 1650, 2093, 2188, 2218
BstMAI GTCTC 5 cut(s) 186, 639, 788, 1389, 1568
BstMBI GATC 8 cut(s) 54, 147, 1390, 1612, 1647, 2090, 2185, 2215
BstMWI GCNNNNNNNGC 5 cut(s) 226, 463, 2291, 2393, 3226
BstNSI RCATGY 2 cut(s) 3039, 3050
BstSFI CTRYAG 3 cut(s) 2268, 2289, 2807
BstSLI GKGCMC 3 cut(s) 454, 678, 1290
BstUI CGCG 4 cut(s) 27, 49, 1284, 1719
BstV1I GCAGC 9 cut(s) 207, 415, 498, 1347, 2272, 2275, 2303, 2545, 2701
BstX2I RGATCY 1 cut(s) 2185
BstXI CCANNNNNNTGG 2 cut(s) 1908, 2570
BstYI RGATCY 1 cut(s) 2185
Bsu36I CCTNAGG 1 cut(s) 2382
BsuI GTATCC 4 cut(s) 3, 399, 1090, 1822
BsuRI GGCC 4 cut(s) 452, 466, 676, 2387
BtgZI GCGATG 1 cut(s) 2406
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 3 cut(s) 159, 607, 2268
BveI ACCTGC 1 cut(s) 2800
Cac8I GCNNGC 5 cut(s) 1286, 1340, 2389, 2699, 3048
CaiI CAGNNNCTG 3 cut(s) 1361, 2074, 2189
CfoI GCGC 2 cut(s) 1535, 2481
Cfr10I RCCGGY 2 cut(s) 565, 2224
Cfr13I GGNCC 7 cut(s) 450, 451, 465, 576, 674, 675, 2222
Cfr9I CCCGGG 2 cut(s) 491, 592
CseI GACGC 1 cut(s) 76
CsiI ACCWGGT 2 cut(s) 504, 2464
Csp6I GTAC 7 cut(s) 166, 214, 272, 1308, 2259, 2376, 3251
CspAI ACCGGT 1 cut(s) 2224
CspCI CAANNNNNGTGG 2 cut(s) 2244, 2279
CviAII CATG 7 cut(s) 173, 1541, 1982, 2884, 3036, 3047, 3099
CviQI GTAC 7 cut(s) 166, 214, 272, 1308, 2259, 2376, 3251
DpnI GATC 8 cut(s) 56, 149, 1392, 1614, 1649, 2092, 2187, 2217
DpnII GATC 8 cut(s) 54, 147, 1390, 1612, 1647, 2090, 2185, 2215
DraI TTTAAA 3 cut(s) 2746, 3007, 3106
Eam1104I CTCTTC 6 cut(s) 33, 1239, 1632, 2417, 2489, 3159
EarI CTCTTC 6 cut(s) 33, 1239, 1632, 2417, 2489, 3159
EciI GGCGGA 2 cut(s) 728, 3235
Ecl136II GAGCTC 1 cut(s) 370
Eco130I CCWWGG 2 cut(s) 678, 3180
Eco24I GRGCYC 3 cut(s) 372, 454, 678
Eco31I GGTCTC 1 cut(s) 1389
Eco32I GATATC 1 cut(s) 2681
Eco47I GGWCC 2 cut(s) 576, 2222
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 2 cut(s) 1155, 1314
Eco72I CACGTG 1 cut(s) 2942
Eco81I CCTNAGG 1 cut(s) 2382
Eco88I CYCGRG 3 cut(s) 491, 592, 1854
EcoICRI GAGCTC 1 cut(s) 370
EcoNI CCTNNNNNAGG 2 cut(s) 2325, 2807
EcoO109I RGGNCCY 3 cut(s) 451, 576, 674
EcoRI GAATTC 2 cut(s) 1756, 1863
EcoRV GATATC 1 cut(s) 2681
EcoT14I CCWWGG 2 cut(s) 678, 3180
EcoT38I GRGCYC 3 cut(s) 372, 454, 678
ErhI CCWWGG 2 cut(s) 678, 3180
Esp3I CGTCTC 1 cut(s) 1568
FaeI CATG 7 cut(s) 176, 1544, 1985, 2887, 3039, 3050, 3102
FalI AAGNNNNNCTT 6 cut(s) 1734, 1766, 2559, 2591, 3015, 3047
FaqI GGGAC 4 cut(s) 788, 920, 1372, 2486
FatI CATG 7 cut(s) 172, 1540, 1981, 2883, 3035, 3046, 3098
FauI CCCGC 1 cut(s) 462
FauNDI CATATG 1 cut(s) 1582
FblI GTMKAC 1 cut(s) 2512
FriOI GRGCYC 3 cut(s) 372, 454, 678
FspBI CTAG 6 cut(s) 927, 1343, 1761, 1815, 2064, 2694
GlaI GCGC 2 cut(s) 1534, 2480
GsaI CCCAGC 2 cut(s) 285, 2574
GsuI CTGGAG 1 cut(s) 1899
HaeIII GGCC 4 cut(s) 452, 466, 676, 2387
HapII CCGG 9 cut(s) 64, 93, 183, 492, 566, 593, 671, 1162, 2225
HgaI GACGC 1 cut(s) 76
HhaI GCGC 2 cut(s) 1535, 2481
Hin1I GRCGYC 2 cut(s) 68, 1577
Hin1II CATG 7 cut(s) 176, 1544, 1985, 2887, 3039, 3050, 3102
Hin6I GCGC 2 cut(s) 1533, 2479
HinP1I GCGC 2 cut(s) 1533, 2479
HincII GTYRAC 2 cut(s) 2937, 3116
HindII GTYRAC 2 cut(s) 2937, 3116
HpaII CCGG 9 cut(s) 64, 93, 183, 492, 566, 593, 671, 1162, 2225
HphI GGTGA 9 cut(s) 16, 49, 552, 797, 2417, 2513, 2824, 2889, 3140
Hpy166II GTNNAC 8 cut(s) 216, 1288, 1926, 2408, 2513, 2937, 2945, 3116
Hpy8I GTNNAC 8 cut(s) 216, 1288, 1926, 2408, 2513, 2937, 2945, 3116
Hpy99I CGWCG 3 cut(s) 76, 2235, 2250
HpyCH4III ACNGT 9 cut(s) 522, 602, 779, 1400, 1526, 1930, 2099, 2246, 2263
HpyCH4IV ACGT 3 cut(s) 1450, 1577, 2941
HpyF10VI GCNNNNNNNGC 5 cut(s) 226, 463, 2291, 2393, 3226
HpySE526I ACGT 3 cut(s) 1450, 1577, 2941
Hsp92I GRCGYC 2 cut(s) 68, 1577
Hsp92II CATG 7 cut(s) 176, 1544, 1985, 2887, 3039, 3050, 3102
HspAI GCGC 2 cut(s) 1533, 2479
Kpn2I TCCGGA 1 cut(s) 1161
Kzo9I GATC 8 cut(s) 54, 147, 1390, 1612, 1647, 2090, 2185, 2215
LguI GCTCTTC 3 cut(s) 1239, 1632, 3159
LmnI GCTCC 3 cut(s) 483, 2518, 2929
Lsp1109I GCAGC 9 cut(s) 207, 415, 498, 1347, 2272, 2275, 2303, 2545, 2701
MabI ACCWGGT 2 cut(s) 504, 2464
MaeI CTAG 6 cut(s) 927, 1343, 1761, 1815, 2064, 2694
MaeII ACGT 3 cut(s) 1450, 1577, 2941
MaeIII GTNAC 8 cut(s) 86, 203, 1563, 1635, 2001, 2029, 2460, 2608
MalI GATC 8 cut(s) 56, 149, 1392, 1614, 1649, 2092, 2187, 2217
MboI GATC 8 cut(s) 54, 147, 1390, 1612, 1647, 2090, 2185, 2215
MfeI CAATTG 2 cut(s) 1605, 1986
MflI RGATCY 1 cut(s) 2185
MhlI GDGCHC 7 cut(s) 372, 454, 678, 1290, 1627, 2178, 2926
MmeI TCCRAC 2 cut(s) 2762, 3020
MroI TCCGGA 1 cut(s) 1161
MroXI GAANNNNTTC 4 cut(s) 1040, 1746, 2640, 2979
MslI CAYNNNNRTG 2 cut(s) 2247, 2550
MspA1I CMGCKG 2 cut(s) 457, 1133
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 9 cut(s) 64, 93, 183, 492, 566, 593, 671, 1162, 2225
MunI CAATTG 2 cut(s) 1605, 1986
Mva1269I GAATGC 1 cut(s) 3018
MvnI CGCG 4 cut(s) 27, 49, 1284, 1719
MwoI GCNNNNNNNGC 5 cut(s) 226, 463, 2291, 2393, 3226
NciI CCSGG 7 cut(s) 64, 184, 492, 493, 593, 594, 672
NdeI CATATG 1 cut(s) 1582
NdeII GATC 8 cut(s) 54, 147, 1390, 1612, 1647, 2090, 2185, 2215
NlaIII CATG 7 cut(s) 176, 1544, 1985, 2887, 3039, 3050, 3102
NlaIV GGNNCC 7 cut(s) 452, 453, 467, 675, 676, 1773, 3029
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 4 cut(s) 86, 1563, 1635, 2001
NspI RCATGY 2 cut(s) 3039, 3050
OliI CACNNNNGTG 1 cut(s) 2247
PaeI GCATGC 1 cut(s) 3050
PaeR7I CTCGAG 1 cut(s) 1854
PaqCI CACCTGC 1 cut(s) 2800
PasI CCCWGGG 1 cut(s) 1908
PciI ACATGT 1 cut(s) 3035
PciSI GCTCTTC 3 cut(s) 1239, 1632, 3159
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PctI GAATGC 1 cut(s) 3018
PdmI GAANNNNTTC 4 cut(s) 1040, 1746, 2640, 2979
PflFI GACNNNGTC 1 cut(s) 21
PfoI TCCNGGA 2 cut(s) 62, 2311
PinAI ACCGGT 1 cut(s) 2224
PmaCI CACGTG 1 cut(s) 2942
PmlI CACGTG 1 cut(s) 2942
Ppu21I YACGTR 1 cut(s) 2942
PpuMI RGGWCCY 1 cut(s) 576
PscI ACATGT 1 cut(s) 3035
Psp124BI GAGCTC 1 cut(s) 372
Psp5II RGGWCCY 1 cut(s) 576
PspCI CACGTG 1 cut(s) 2942
PspFI CCCAGC 2 cut(s) 281, 2570
PspN4I GGNNCC 7 cut(s) 452, 453, 467, 675, 676, 1773, 3029
PspOMI GGGCCC 2 cut(s) 450, 674
PspPI GGNCC 7 cut(s) 450, 451, 465, 576, 674, 675, 2222
PspPPI RGGWCCY 1 cut(s) 576
PstI CTGCAG 2 cut(s) 2293, 2811
PstNI CAGNNNCTG 3 cut(s) 1361, 2074, 2189
PsuI RGATCY 1 cut(s) 2185
PsyI GACNNNGTC 1 cut(s) 21
PvuII CAGCTG 1 cut(s) 1133
RsaI GTAC 7 cut(s) 167, 215, 273, 1309, 2260, 2377, 3252
RsaNI GTAC 7 cut(s) 166, 214, 272, 1308, 2259, 2376, 3251
RseI CAYNNNNRTG 2 cut(s) 2247, 2550
SacI GAGCTC 1 cut(s) 372
SapI GCTCTTC 3 cut(s) 1239, 1632, 3159
Sau3AI GATC 8 cut(s) 54, 147, 1390, 1612, 1647, 2090, 2185, 2215
Sau96I GGNCC 7 cut(s) 450, 451, 465, 576, 674, 675, 2222
ScaI AGTACT 2 cut(s) 1309, 3252
SduI GDGCHC 7 cut(s) 372, 454, 678, 1290, 1627, 2178, 2926
SexAI ACCWGGT 2 cut(s) 504, 2464
SfcI CTRYAG 3 cut(s) 2268, 2289, 2807
Sfr274I CTCGAG 1 cut(s) 1854
SinI GGWCC 2 cut(s) 576, 2222
SlaI CTCGAG 1 cut(s) 1854
SmaI CCCGGG 2 cut(s) 493, 594
SmiMI CAYNNNNRTG 2 cut(s) 2247, 2550
SmlI CTYRAG 4 cut(s) 329, 341, 1854, 2951
SmoI CTYRAG 4 cut(s) 329, 341, 1854, 2951
SphI GCATGC 1 cut(s) 3050
SsiI CCGC 6 cut(s) 27, 47, 455, 713, 1282, 3220
SspI AATATT 1 cut(s) 3264
SspMI CTAG 6 cut(s) 927, 1343, 1761, 1815, 2064, 2694
SstI GAGCTC 1 cut(s) 372
StyI CCWWGG 2 cut(s) 678, 3180
TaaI ACNGT 9 cut(s) 522, 602, 779, 1400, 1526, 1930, 2099, 2246, 2263
TaiI ACGT 3 cut(s) 1453, 1580, 2944
TaqI TCGA 8 cut(s) 123, 190, 372, 803, 1124, 1393, 1855, 2230
TatI WGTACW 7 cut(s) 165, 213, 271, 1307, 2258, 2375, 3250
TauI GCSGC 2 cut(s) 30, 49
TscAI CASTG 3 cut(s) 159, 607, 2268
TseFI GTSAC 4 cut(s) 86, 1563, 1635, 2001
TseI GCWGC 9 cut(s) 220, 428, 486, 1335, 2285, 2288, 2291, 2533, 2714
Tsp45I GTSAC 4 cut(s) 86, 1563, 1635, 2001
TspGWI ACGGA 3 cut(s) 1364, 2248, 3133
TspMI CCCGGG 2 cut(s) 491, 592
TspRI CASTG 3 cut(s) 159, 607, 2268
Tth111I GACNNNGTC 1 cut(s) 21
Vha464I CTTAAG 1 cut(s) 329
VneI GTGCAC 1 cut(s) 1286
VpaK11BI GGWCC 2 cut(s) 576, 2222
XagI CCTNNNNNAGG 2 cut(s) 2325, 2807
XapI RAATTY 7 cut(s) 1052, 1756, 1809, 1863, 2024, 2731, 2911
XceI RCATGY 2 cut(s) 3039, 3050
XcmI CCANNNNNNNNNTGG 2 cut(s) 289, 476
XhoI CTCGAG 1 cut(s) 1854
XmaI CCCGGG 2 cut(s) 491, 592
XmiI GTMKAC 1 cut(s) 2512
XmnI GAANNNNTTC 4 cut(s) 1040, 1746, 2640, 2979
XspI CTAG 6 cut(s) 927, 1343, 1761, 1815, 2064, 2694
ZraI GACGTC 1 cut(s) 1578
ZrmI AGTACT 2 cut(s) 1309, 3252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.