pycom09g17610

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012264
Physical Location & Seq
Reverse (-)
18143882 .. 18157449
13568 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g17610.3

Sequence Viewer

Length: 3273 bp
ATGGATGGCACCATTTTAAAACTCCGCCTCGGGTCCTCAAAAGTCCAGGGAGAGTCGCCCCGTGAGATCACCTCTCGGACGAACCTGTCGAGTGACACCGGTGAGAATGGGTGGCTCATACGGTTCTTTGACTCAGCATTTTTCTGCGAATGGATCGCTGTTAGCTACCTGTACAAGCATGATCACGCGGGGGTGCGTGACTACCTCTGTAATCGAATGTACACTCTTCCCTTATCAGGTATCGAGAGCTACTTGTTCCAGATATGTTACATGTCAGTGCACAAGCCTAGCCCATCTTTGGATAAGTTTGTTATTGATATGTGCTCTAAGTCGCTTAAGATAGCACTGAAGGTGCATTGGTTTTTATTGGCGGAGCTTGAAGACTCAGATGATAATGAAGGAATTAATAGGATTCAGGAGAAGTGTCAGATTGCAGCCACTTTGATGGGTGAGTGGCCACCTTTGGTACCTCCTCAAAATGAGTCAGCTAGCCCTGGGAGCAAGAACCAGGTATTGAATAGAATATTGTCATCAAAACAGAAATTGTTGTCACTAACGTCTTCACCACCTGCCCAGAGGTCTTTTTCTTTCTCGCCTTCATCGGGAAACAATCTGCAGGAGGATGGTGCTCCCTTCTCTCCCGATGAGAACAAAATTTTTAAAAAATTCATTCCAGGGCCAAAGGTTCGGGATGCATTGCTCTTTAGAAAGTCAGTGGAGAAAGATGATGATGACTCTGAAAAAGATGGGTTCTTTAAGAGGCTTTTAAGAGACAGTAGAGGTGATGATGAGACAGGTTCTAAGCTTAGAGATTCTTTGCTCTTTAGGAAGTCCTCAGAGAAGGATGATGATGCAGGGAAGGATGGCTTCTTTAAGAGGCTATTAAGGGATAGTAAAGGCGACGATGAGGAATTGACATCAAGCTCAGAGGGGTTTTTTAAGAGATTGTTTCGTGATAGTAAGAGTGATTCTGATGATAAATCAATCTCTAAATCAGTGGACGATGAAGAAAAAGAAGGTTTCTTCCGGAAGCTTTTCAAAGATAAGTCTGAGGACAAGAAGGACCGGATTGATAGGAATGAGGATGAAGATGCCCCATACTCTGAAGAAAAAGGTTCGAAATCTGCTGAAGATGATGAAAAAGAGGGGTTCTTCCGAAAATTCTTCAGGGATAAGTTTGACGACAAGAAAGATGGGAATGATAAAACTGAGGAAGGGAGTGCCAATGGAGATGAAGAAGAGCCGTCTGACTTTTCATTGTTTCGAAGATTGTTCCGTGTGCATCCTGAAGATGCGAAAAGTATTGCTGCAAATGAAAACAGCAGTACTGGTGGCTTGCCAGAAAGTAGTCCAGGGACTGAGAATTTTTTTCGCAAATTGTTTAGAGATCGTGACCGTTCAGTTGAAGACTCGGAGCTATTTGGTTCAAGAAGACACAAAGAGAAACGCCCTGGTTCACCAAAGCAACAAAATGAAAAGTCAAGTGTGAAACCCCCACTTCCAAATGGTACAACATCCCAATACCGTAAAGGGGCTTATCATGAGTCGCTGGACTTTGTGCAGTCACTATGTGACACGTCTTATGGCTTAGTGGATATATTTCCAATTGAGGATCGCAAGAGTGCTCTTCGTGAGTCACTTGCGGAGATCAATTTGCACATTGCTGAAGCTCAAAATAGTGGAGGAGTTTGCTTTCCAATGGGGAAGGGGATGTATCGCGTGGTTTATATACCAGAAGATGAAGCTGTTCTTTTGAATTCTAGGGAAAAGGCACCGTATCTCATATGTGTTGAAGTCCTGAAAAGTGAAATTACAGGCAATGCAAAGGACATATCTGGTTCTCAAAAGCTTTCTCGAGGGGGAATTCCTTTAGCAAATGGGGATGCACTTCTGCAAAGACCGCCTCCGTGGGCATATCCTTTGTGGACTGTCCAGGAGGTCTATCGCAATAGTAATGATAGGATGTCGAGTTCAACCGCTCAAGCAATTGACCAGGCAATGTCACATACGTCCGAGGCAAAAGCGACATTTGTTACTGTAAAAATATCTGTGGAGAATAAATTGCATAGTCATACAGTAAAAGCAGAGAATATATCAAGGAATTATCAACGTAGAGAGGCTGCGACTTGTGCATCAAAAGAAGCACAAGAGAGTGATTTGCAGTGGGTGAGGGTGGTGCTTACAGCAGATCCTGGTGTTAGGATGGAGGACATTGAGGATCAAGGCCCACCTCGGCGGAAGGAACACCGTCGTGTTCCAAGTACAGTGGCTATAGAGGAAGCAAAGGCTGCTGCAGCAAAAGGAGAAGCACCTCCTGGACTTCCTCTGAAAGGGGCTGGTCAGGATTCATCAGATGCACAGCCAATGGCTAATGGTGGTACTCCCAAAGCCAGCAATGCCTTGTCTGGCGAACTTTGGGAGGTAAAGAAGGAGAGATTAAGAAAAGCTTCAGTTCATGGAAAGTTACCTGGTTGGGACTTGCAGTCGGTTATCGTGAAGAGTGGTGATGATTGTAGGCAGGAGCATCTTGCTGTGCAACTTATTTCTCACTTTTATGATATATTCCAAGAAGCTGGTCTTCCTCTCTGGTTGCGCCCTTATGAAATCTTAGTTACTTCTTCTTACACAGCTCTCATTGAAACAATTCCAGATACGGCTTCAATTCATTCTATCAAAAGTAGATATCCGAACATCACGAGCTTGCGAGACTTCTTTGCTGCTAAGTACCAAGAAAATTCTCCAAGTTTTAAACTTGCTCAGAGGAATTTTGTTGAAAGTATGGCTGGGTATTCTCTAGTTTGTTACCTTCTGCAGATAAAGGATAGGCACAATGGGAACCTCTTAATGGATGAAGAAGGTCATATTGTACATATTGATTTCGGTTTCATGCTCTCCAATTCGCCTGGGGGTGTAAATTTTGAGAGTGCTCCATTCAAATTAACACGTGAACTTCTTGAGGTCATGGATTCTGATGCTGAGGGAGTTCCTAGCGAGTTCTTTGATTATTTTAAGGTTTTGTGCATTCAAGGGTTCCTTACATGTCGGAAGCATGCAGAACGCATTATTCTTCTTGTTGAGATGTTGCAGGACTCTGGTTTTCCATGCTTTAAAGGTGGTGCACGAACAATACAGAATCTGCGGAAACGGTTTCACTTGAGTTTAACAGAAGAGCAATGCGTATCCTTGGTGCTTTCTTTGATCAGCAGCAGCTTGGATGCATGGCGAACAAGGCAGTATGATTATTATCAGAGGGTTTTGAACGGAATATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012506 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017137 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0030258 GO:0030659 GO:0031090 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0035618 GO:0035619 GO:0040007 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044706 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048015 GO:0048017 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051704 GO:0051716 GO:0052742 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0097708 GO:0099402 GO:0120025 GO:0120038 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

1091

Amino Acids

122.78

Weight (kDa)

5.75

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09350 AT5G64070 AT5G64070
fragaria_vesca FvH4_1g24240
malus_domestica MD09G1261800.v1.1 MD17G1256600.v1.1
prunus_persica Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1
pyrus_communis pycom09g17610 pycom17g25880
rosa_chinensis RchiOBHm_Chr2g0120731
rosa_laevigata RLG00000018575
rosa_multiflora Rmu_sc0000319.1_g000011
rosa_roxburghii Rroxscaffold_2G00123200
rosa_rugosa Rorug02G0230500
rosa_samantha Rh2BG298500 Rh2CG276200
rosa_wichuraiana Rw2G023030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 577
AasI GACNNNNNNGTC 1 cut(s) 85
Acc36I ACCTGC 1 cut(s) 577
Acc65I GGTACC 1 cut(s) 466
AccB1I GGYRCC 3 cut(s) 8, 466, 1771
AccBSI CCGCTC 1 cut(s) 1979
AccII CGCG 2 cut(s) 188, 1719
AccIII TCCGGA 1 cut(s) 1026
AciI CCGC 8 cut(s) 25, 188, 371, 1643, 1901, 1977, 2236, 3139
AclWI GGATC 4 cut(s) 161, 1620, 2183, 2226
AcoI YGGCCR 1 cut(s) 455
AcsI RAATTY 9 cut(s) 654, 665, 1160, 1363, 1756, 1863, 2734, 2764, 2914
AcuI CTGAAG 7 cut(s) 368, 1125, 1149, 1150, 1308, 1686, 2433
AcvI CACGTG 1 cut(s) 2945
AdeI CACNNNGTG 1 cut(s) 1571
AfaI GTAC 9 cut(s) 173, 221, 468, 1327, 1510, 2263, 2380, 2726, 2868
AfiI CCNNNNNNNGG 6 cut(s) 236, 298, 602, 1531, 2330, 2845
AflII CTTAAG 1 cut(s) 335
AflIII ACRYGT 4 cut(s) 270, 1575, 2942, 3038
AgeI ACCGGT 1 cut(s) 98
AhdI GACNNNNNGTC 1 cut(s) 2482
AjiI CACGTC 1 cut(s) 1578
AleI CACNNNNGTG 1 cut(s) 2250
AloI GAACNNNNNNTCC 2 cut(s) 1954, 1986
Alw21I GWGCWC 6 cut(s) 282, 326, 631, 1627, 2929, 3121
Alw26I GTCTC 3 cut(s) 765, 785, 2700
Alw44I GTGCAC 2 cut(s) 278, 3117
AlwI GGATC 4 cut(s) 161, 1620, 2183, 2226
AlwNI CAGNNNCTG 3 cut(s) 1358, 2192, 3136
Ama87I CYCGRG 2 cut(s) 29, 1854
Aor13HI TCCGGA 1 cut(s) 1026
AoxI GGCC 3 cut(s) 455, 677, 2224
ApaLI GTGCAC 2 cut(s) 278, 3117
ApeKI GCWGC 9 cut(s) 434, 1307, 2120, 2288, 2291, 2294, 2717, 3204, 3207
ApoI RAATTY 9 cut(s) 654, 665, 1160, 1363, 1756, 1863, 2734, 2764, 2914
AseI ATTAAT 1 cut(s) 405
AsiGI ACCGGT 1 cut(s) 98
Asp700I GAANNNNTTC 4 cut(s) 1034, 1746, 2446, 2643
Asp718I GGTACC 1 cut(s) 466
AspLEI GCGC 1 cut(s) 2595
AspS9I GGNCC 4 cut(s) 33, 677, 1063, 2225
AsuHPI GGTGA 8 cut(s) 61, 113, 461, 555, 794, 1449, 2179, 2516
AsuII TTCGAA 2 cut(s) 1118, 1264
AsuNHI GCTAGC 1 cut(s) 488
AvaI CYCGRG 2 cut(s) 29, 1854
AvaII GGWCC 2 cut(s) 33, 1063
BaeGI GKGCMC 2 cut(s) 282, 3121
BalI TGGCCA 1 cut(s) 457
BanI GGYRCC 3 cut(s) 8, 466, 1771
BauI CACGAG 1 cut(s) 2695
BbrPI CACGTG 1 cut(s) 2945
BbsI GAAGAC 5 cut(s) 387, 552, 1413, 1438, 2570
Bbv12I GWGCWC 6 cut(s) 282, 326, 631, 1627, 2929, 3121
BbvCI CCTCAGC 1 cut(s) 2976
BbvI GCAGC 9 cut(s) 446, 1294, 2107, 2275, 2278, 2306, 2704, 3216, 3219
BccI CCATC 7 cut(s) 301, 439, 617, 740, 857, 1187, 2197
BceAI ACGGC 2 cut(s) 1228, 2670
BciVI GTATCC 1 cut(s) 3190
BclI TGATCA 2 cut(s) 181, 3198
BcoDI GTCTC 3 cut(s) 765, 785, 2700
BfaI CTAG 5 cut(s) 288, 489, 1761, 2795, 2988
BfmI CTRYAG 4 cut(s) 614, 2271, 2292, 2810
BfrI CTTAAG 1 cut(s) 335
BfuAI ACCTGC 1 cut(s) 577
BfuI GTATCC 1 cut(s) 3190
BisI GCNGC 9 cut(s) 435, 1308, 2121, 2289, 2292, 2295, 2718, 3205, 3208
BlsI GCNGC 9 cut(s) 436, 1309, 2122, 2290, 2293, 2296, 2719, 3206, 3209
BmcAI AGTACT 1 cut(s) 1327
Bme18I GGWCC 2 cut(s) 33, 1063
BmeRI GACNNNNNGTC 1 cut(s) 2482
BmeT110I CYCGRG 2 cut(s) 29, 1854
BmgBI CACGTC 1 cut(s) 1578
BmgT120I GGNCC 4 cut(s) 33, 677, 1063, 2225
BmiI GGNNCC 6 cut(s) 10, 34, 468, 1773, 2837, 3032
BmtI GCTAGC 1 cut(s) 492
BpiI GAAGAC 5 cut(s) 387, 552, 1413, 1438, 2570
BplI GAGNNNNNCTC 2 cut(s) 56, 88
Bpu10I CCTNAGC 1 cut(s) 2976
Bpu14I TTCGAA 2 cut(s) 1118, 1264
BpuEI CTTGAG 3 cut(s) 1965, 2975, 3175
BsaAI YACGTR 1 cut(s) 2945
BsaBI GATNNNNATC 1 cut(s) 3243
BsaWI WCCGGW 3 cut(s) 98, 1026, 1065
Bsc4I CCNNNNNNNGG 6 cut(s) 236, 298, 602, 1531, 2330, 2845
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 1 cut(s) 1333
Bse3DI GCAATG 6 cut(s) 695, 1659, 1825, 2004, 2401, 3179
Bse8I GATNNNNATC 1 cut(s) 3243
BseAI TCCGGA 1 cut(s) 1026
BseJI GATNNNNATC 1 cut(s) 3243
BseLI CCNNNNNNNGG 6 cut(s) 236, 298, 602, 1531, 2330, 2845
BseMI GCAATG 6 cut(s) 695, 1659, 1825, 2004, 2401, 3179
BseMII CTCAG 9 cut(s) 147, 399, 849, 939, 1041, 1200, 1350, 2771, 2967
BseNI ACTGG 1 cut(s) 1333
BseRI GAGGAG 2 cut(s) 462, 1698
BseSI GKGCMC 2 cut(s) 282, 3121
BseXI GCAGC 9 cut(s) 446, 1294, 2107, 2275, 2278, 2306, 2704, 3216, 3219
BseYI CCCAGC 1 cut(s) 2783
BsgI GTGCAG 1 cut(s) 1580
Bsh1236I CGCG 2 cut(s) 188, 1719
BshFI GGCC 3 cut(s) 457, 679, 2226
BshNI GGYRCC 3 cut(s) 8, 466, 1771
BshTI ACCGGT 1 cut(s) 98
BsiHKAI GWGCWC 6 cut(s) 282, 326, 631, 1627, 2929, 3121
BsiHKCI CYCGRG 2 cut(s) 29, 1854
BsiSI CCGG 3 cut(s) 99, 1027, 1066
BslFI GGGAC 2 cut(s) 1369, 2489
BslI CCNNNNNNNGG 6 cut(s) 236, 298, 602, 1531, 2330, 2845
BsmAI GTCTC 3 cut(s) 765, 785, 2700
BsmFI GGGAC 2 cut(s) 1369, 2489
BsmI GAATGC 1 cut(s) 3021
BsnI GGCC 3 cut(s) 457, 679, 2226
BsoBI CYCGRG 2 cut(s) 29, 1854
Bsp119I TTCGAA 2 cut(s) 1118, 1264
Bsp1286I GDGCHC 6 cut(s) 282, 326, 631, 1627, 2929, 3121
Bsp13I TCCGGA 1 cut(s) 1026
Bsp1407I TGTACA 3 cut(s) 171, 219, 2866
Bsp143I GATC 9 cut(s) 66, 153, 181, 1387, 1612, 1647, 2188, 2218, 3198
BspACI CCGC 8 cut(s) 25, 188, 371, 1643, 1901, 1977, 2236, 3139
BspANI GGCC 3 cut(s) 457, 679, 2226
BspCNI CTCAG 9 cut(s) 146, 398, 848, 938, 1042, 1201, 1351, 2770, 2968
BspEI TCCGGA 1 cut(s) 1026
BspFNI CGCG 2 cut(s) 188, 1719
BspHI TCATGA 1 cut(s) 1540
BspLI GGNNCC 6 cut(s) 10, 34, 468, 1773, 2837, 3032
BspMAI CTGCAG 3 cut(s) 618, 2296, 2814
BspMI ACCTGC 1 cut(s) 577
BspOI GCTAGC 1 cut(s) 492
BspPI GGATC 4 cut(s) 161, 1620, 2183, 2226
BspQI GCTCTTC 3 cut(s) 1233, 1632, 3162
BspT104I TTCGAA 2 cut(s) 1118, 1264
BspT107I GGYRCC 3 cut(s) 8, 466, 1771
BspTI CTTAAG 1 cut(s) 335
BsrBI CCGCTC 1 cut(s) 1979
BsrDI GCAATG 6 cut(s) 695, 1659, 1825, 2004, 2401, 3179
BsrFI RCCGGY 1 cut(s) 98
BsrGI TGTACA 3 cut(s) 171, 219, 2866
BsrI ACTGG 1 cut(s) 1333
BssAI RCCGGY 1 cut(s) 98
BssMI GATC 9 cut(s) 66, 153, 181, 1387, 1612, 1647, 2188, 2218, 3198
BssSI CACGAG 1 cut(s) 2695
BssT1I CCWWGG 1 cut(s) 3183
Bst2BI CACGAG 1 cut(s) 2695
Bst6I CTCTTC 5 cut(s) 231, 1233, 1632, 2492, 3162
BstAFI CTTAAG 1 cut(s) 335
BstAPI GCANNNNNTGC 1 cut(s) 2288
BstAUI TGTACA 3 cut(s) 171, 219, 2866
BstBAI YACGTR 1 cut(s) 2945
BstBI TTCGAA 2 cut(s) 1118, 1264
BstC8I GCNNGC 5 cut(s) 490, 1337, 2392, 2702, 3051
BstDSI CCRYGG 1 cut(s) 1907
BstENI CCTNNNNNAGG 1 cut(s) 2328
BstFNI CGCG 2 cut(s) 188, 1719
BstHHI GCGC 1 cut(s) 2595
BstKTI GATC 9 cut(s) 69, 156, 184, 1390, 1615, 1650, 2191, 2221, 3201
BstMAI GTCTC 3 cut(s) 765, 785, 2700
BstMBI GATC 9 cut(s) 66, 153, 181, 1387, 1612, 1647, 2188, 2218, 3198
BstMWI GCNNNNNNNGC 7 cut(s) 498, 1900, 2129, 2288, 2294, 2396, 3229
BstNSI RCATGY 3 cut(s) 274, 3042, 3053
BstSFI CTRYAG 4 cut(s) 614, 2271, 2292, 2810
BstSLI GKGCMC 2 cut(s) 282, 3121
BstUI CGCG 2 cut(s) 188, 1719
BstV1I GCAGC 9 cut(s) 446, 1294, 2107, 2275, 2278, 2306, 2704, 3216, 3219
BstV2I GAAGAC 5 cut(s) 387, 552, 1413, 1438, 2570
BstX2I RGATCY 1 cut(s) 2188
BstXI CCANNNNNNTGG 2 cut(s) 445, 2573
BstYI RGATCY 1 cut(s) 2188
BsuI GTATCC 1 cut(s) 3190
BsuRI GGCC 3 cut(s) 457, 679, 2226
BtgI CCRYGG 1 cut(s) 1907
BtrI CACGTC 1 cut(s) 1578
BtsI GCAGTG 1 cut(s) 2168
BtsIMutI CAGTG 6 cut(s) 282, 344, 720, 1002, 2168, 2271
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 5 cut(s) 490, 1337, 2392, 2702, 3051
CaiI CAGNNNCTG 3 cut(s) 1358, 2192, 3136
CciI TCATGA 1 cut(s) 1540
CfoI GCGC 1 cut(s) 2595
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 4 cut(s) 33, 677, 1063, 2225
CsiI ACCWGGT 2 cut(s) 507, 2467
Csp6I GTAC 9 cut(s) 172, 220, 467, 1326, 1509, 2262, 2379, 2725, 2867
CspAI ACCGGT 1 cut(s) 98
CspCI CAANNNNNGTGG 2 cut(s) 2247, 2282
CviQI GTAC 9 cut(s) 172, 220, 467, 1326, 1509, 2262, 2379, 2725, 2867
DpnI GATC 9 cut(s) 68, 155, 183, 1389, 1614, 1649, 2190, 2220, 3200
DpnII GATC 9 cut(s) 66, 153, 181, 1387, 1612, 1647, 2188, 2218, 3198
DraI TTTAAA 4 cut(s) 18, 661, 2749, 3109
DraIII CACNNNGTG 1 cut(s) 1571
DrdI GACNNNNNNGTC 1 cut(s) 85
DriI GACNNNNNGTC 1 cut(s) 2482
DseDI GACNNNNNNGTC 1 cut(s) 85
EaeI YGGCCR 1 cut(s) 455
Eam1104I CTCTTC 5 cut(s) 231, 1233, 1632, 2492, 3162
Eam1105I GACNNNNNGTC 1 cut(s) 2482
EarI CTCTTC 5 cut(s) 231, 1233, 1632, 2492, 3162
EciI GGCGGA 3 cut(s) 14, 386, 2251
Eco130I CCWWGG 1 cut(s) 3183
Eco32I GATATC 1 cut(s) 2684
Eco47I GGWCC 2 cut(s) 33, 1063
Eco57I CTGAAG 7 cut(s) 368, 1125, 1149, 1150, 1308, 1686, 2433
Eco72I CACGTG 1 cut(s) 2945
Eco88I CYCGRG 2 cut(s) 29, 1854
EcoNI CCTNNNNNAGG 1 cut(s) 2328
EcoO109I RGGNCCY 1 cut(s) 33
EcoRI GAATTC 2 cut(s) 1756, 1863
EcoRV GATATC 1 cut(s) 2684
EcoT14I CCWWGG 1 cut(s) 3183
EcoT22I ATGCAT 2 cut(s) 697, 3220
ErhI CCWWGG 1 cut(s) 3183
FaqI GGGAC 2 cut(s) 1369, 2489
FauI CCCGC 1 cut(s) 181
FauNDI CATATG 1 cut(s) 1784
FbaI TGATCA 2 cut(s) 181, 3198
Fnu4HI GCNGC 9 cut(s) 435, 1308, 2121, 2289, 2292, 2295, 2718, 3205, 3208
Fsp4HI GCNGC 9 cut(s) 435, 1308, 2121, 2289, 2292, 2295, 2718, 3205, 3208
FspBI CTAG 5 cut(s) 288, 489, 1761, 2795, 2988
GlaI GCGC 1 cut(s) 2594
GluI GCNGC 9 cut(s) 435, 1308, 2121, 2289, 2292, 2295, 2718, 3205, 3208
GsaI CCCAGC 1 cut(s) 2787
HaeIII GGCC 3 cut(s) 457, 679, 2226
HapII CCGG 3 cut(s) 99, 1027, 1066
HhaI GCGC 1 cut(s) 2595
Hin6I GCGC 1 cut(s) 2593
HinP1I GCGC 1 cut(s) 2593
HindIII AAGCTT 4 cut(s) 803, 1031, 1847, 2445
HpaII CCGG 3 cut(s) 99, 1027, 1066
HphI GGTGA 8 cut(s) 61, 113, 461, 555, 794, 1449, 2179, 2516
Hpy166II GTNNAC 7 cut(s) 222, 280, 1000, 1457, 1926, 2948, 3119
Hpy8I GTNNAC 7 cut(s) 222, 280, 1000, 1457, 1926, 2948, 3119
Hpy99I CGWCG 2 cut(s) 905, 2253
HpyCH4IV ACGT 5 cut(s) 557, 1577, 2009, 2110, 2944
HpyF10VI GCNNNNNNNGC 7 cut(s) 498, 1900, 2129, 2288, 2294, 2396, 3229
HpySE526I ACGT 5 cut(s) 557, 1577, 2009, 2110, 2944
HspAI GCGC 1 cut(s) 2593
Kpn2I TCCGGA 1 cut(s) 1026
KpnI GGTACC 1 cut(s) 470
Ksp22I TGATCA 2 cut(s) 181, 3198
Kzo9I GATC 9 cut(s) 66, 153, 181, 1387, 1612, 1647, 2188, 2218, 3198
LguI GCTCTTC 3 cut(s) 1233, 1632, 3162
LmnI GCTCC 6 cut(s) 373, 498, 634, 1414, 2521, 2932
Lsp1109I GCAGC 9 cut(s) 446, 1294, 2107, 2275, 2278, 2306, 2704, 3216, 3219
MabI ACCWGGT 2 cut(s) 507, 2467
MaeI CTAG 5 cut(s) 288, 489, 1761, 2795, 2988
MaeII ACGT 5 cut(s) 557, 1577, 2009, 2110, 2944
MalI GATC 9 cut(s) 68, 155, 183, 1389, 1614, 1649, 2190, 2220, 3200
MbiI CCGCTC 1 cut(s) 1979
MboI GATC 9 cut(s) 66, 153, 181, 1387, 1612, 1647, 2188, 2218, 3198
MfeI CAATTG 2 cut(s) 1605, 1986
MflI RGATCY 1 cut(s) 2188
MhlI GDGCHC 6 cut(s) 282, 326, 631, 1627, 2929, 3121
MlsI TGGCCA 1 cut(s) 457
MluNI TGGCCA 1 cut(s) 457
MlyI GAGTC 9 cut(s) 62, 125, 377, 491, 728, 1403, 1553, 1643, 3083
MmeI TCCRAC 1 cut(s) 3023
Mox20I TGGCCA 1 cut(s) 457
Mph1103I ATGCAT 2 cut(s) 697, 3220
MroI TCCGGA 1 cut(s) 1026
MroXI GAANNNNTTC 4 cut(s) 1034, 1746, 2446, 2643
MscI TGGCCA 1 cut(s) 457
MslI CAYNNNNRTG 4 cut(s) 275, 443, 2250, 2553
Msp20I TGGCCA 1 cut(s) 457
MspCI CTTAAG 1 cut(s) 335
MspI CCGG 3 cut(s) 99, 1027, 1066
MunI CAATTG 2 cut(s) 1605, 1986
Mva1269I GAATGC 1 cut(s) 3021
MvnI CGCG 2 cut(s) 188, 1719
MwoI GCNNNNNNNGC 7 cut(s) 498, 1900, 2129, 2288, 2294, 2396, 3229
NdeI CATATG 1 cut(s) 1784
NdeII GATC 9 cut(s) 66, 153, 181, 1387, 1612, 1647, 2188, 2218, 3198
NheI GCTAGC 1 cut(s) 488
NlaIV GGNNCC 6 cut(s) 10, 34, 468, 1773, 2837, 3032
NmeAIII GCCGAG 1 cut(s) 2212
NmuCI GTSAC 8 cut(s) 92, 197, 549, 1391, 1563, 1571, 1635, 2001
NsiI ATGCAT 2 cut(s) 697, 3220
NspI RCATGY 3 cut(s) 274, 3042, 3053
NspV TTCGAA 2 cut(s) 1118, 1264
OliI CACNNNNGTG 1 cut(s) 2250
PaeI GCATGC 1 cut(s) 3053
PaeR7I CTCGAG 1 cut(s) 1854
PagI TCATGA 1 cut(s) 1540
PaqCI CACCTGC 1 cut(s) 577
PasI CCCWGGG 1 cut(s) 494
PciI ACATGT 2 cut(s) 270, 3038
PciSI GCTCTTC 3 cut(s) 1233, 1632, 3162
PcsI WCGNNNNNNNCGW 1 cut(s) 86
PctI GAATGC 1 cut(s) 3021
PdmI GAANNNNTTC 4 cut(s) 1034, 1746, 2446, 2643
PfeI GAWTC 6 cut(s) 412, 812, 968, 2345, 2966, 3133
PfoI TCCNGGA 2 cut(s) 1932, 2314
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 9 cut(s) 436, 1309, 2122, 2290, 2293, 2296, 2719, 3206, 3209
PleI GAGTC 9 cut(s) 61, 125, 377, 490, 728, 1403, 1552, 1642, 3083
PmaCI CACGTG 1 cut(s) 2945
PmlI CACGTG 1 cut(s) 2945
PpsI GAGTC 9 cut(s) 61, 125, 377, 490, 728, 1403, 1552, 1642, 3083
Ppu21I YACGTR 1 cut(s) 2945
PpuMI RGGWCCY 1 cut(s) 33
PscI ACATGT 2 cut(s) 270, 3038
PshBI ATTAAT 1 cut(s) 405
Psp5II RGGWCCY 1 cut(s) 33
PspCI CACGTG 1 cut(s) 2945
PspFI CCCAGC 1 cut(s) 2783
PspN4I GGNNCC 6 cut(s) 10, 34, 468, 1773, 2837, 3032
PspPI GGNCC 4 cut(s) 33, 677, 1063, 2225
PspPPI RGGWCCY 1 cut(s) 33
PstI CTGCAG 3 cut(s) 618, 2296, 2814
PstNI CAGNNNCTG 3 cut(s) 1358, 2192, 3136
PsuI RGATCY 1 cut(s) 2188
RsaI GTAC 9 cut(s) 173, 221, 468, 1327, 1510, 2263, 2380, 2726, 2868
RsaNI GTAC 9 cut(s) 172, 220, 467, 1326, 1509, 2262, 2379, 2725, 2867
RseI CAYNNNNRTG 4 cut(s) 275, 443, 2250, 2553
SapI GCTCTTC 3 cut(s) 1233, 1632, 3162
SatI GCNGC 9 cut(s) 435, 1308, 2121, 2289, 2292, 2295, 2718, 3205, 3208
Sau3AI GATC 9 cut(s) 66, 153, 181, 1387, 1612, 1647, 2188, 2218, 3198
Sau96I GGNCC 4 cut(s) 33, 677, 1063, 2225
ScaI AGTACT 1 cut(s) 1327
SchI GAGTC 9 cut(s) 62, 125, 377, 491, 728, 1403, 1553, 1643, 3083
SduI GDGCHC 6 cut(s) 282, 326, 631, 1627, 2929, 3121
SexAI ACCWGGT 2 cut(s) 507, 2467
SfcI CTRYAG 4 cut(s) 614, 2271, 2292, 2810
Sfr274I CTCGAG 1 cut(s) 1854
SfuI TTCGAA 2 cut(s) 1118, 1264
SgrAI CRCCGGYG 1 cut(s) 98
SinI GGWCC 2 cut(s) 33, 1063
SlaI CTCGAG 1 cut(s) 1854
SmiMI CAYNNNNRTG 4 cut(s) 275, 443, 2250, 2553
SmlI CTYRAG 5 cut(s) 335, 1854, 1980, 2954, 3154
SmoI CTYRAG 5 cut(s) 335, 1854, 1980, 2954, 3154
SphI GCATGC 1 cut(s) 3053
SsiI CCGC 8 cut(s) 25, 188, 371, 1643, 1901, 1977, 2236, 3139
SspI AATATT 2 cut(s) 525, 3267
SspMI CTAG 5 cut(s) 288, 489, 1761, 2795, 2988
StyI CCWWGG 1 cut(s) 3183
TaiI ACGT 5 cut(s) 560, 1580, 2012, 2113, 2947
TaqI TCGA 7 cut(s) 89, 214, 243, 1118, 1264, 1855, 1967
TatI WGTACW 5 cut(s) 171, 219, 1325, 2261, 2866
TfiI GAWTC 6 cut(s) 412, 812, 968, 2345, 2966, 3133
TscAI CASTG 6 cut(s) 282, 351, 720, 1002, 2168, 2271
TseFI GTSAC 8 cut(s) 92, 197, 549, 1391, 1563, 1571, 1635, 2001
TseI GCWGC 9 cut(s) 434, 1307, 2120, 2288, 2291, 2294, 2717, 3204, 3207
Tsp45I GTSAC 8 cut(s) 92, 197, 549, 1391, 1563, 1571, 1635, 2001
TspGWI ACGGA 2 cut(s) 1265, 1896
TspRI CASTG 6 cut(s) 282, 351, 720, 1002, 2168, 2271
Vha464I CTTAAG 1 cut(s) 335
VneI GTGCAC 2 cut(s) 278, 3117
VpaK11BI GGWCC 2 cut(s) 33, 1063
VspI ATTAAT 1 cut(s) 405
XagI CCTNNNNNAGG 1 cut(s) 2328
XapI RAATTY 9 cut(s) 654, 665, 1160, 1363, 1756, 1863, 2734, 2764, 2914
XceI RCATGY 3 cut(s) 274, 3042, 3053
XhoI CTCGAG 1 cut(s) 1854
XmnI GAANNNNTTC 4 cut(s) 1034, 1746, 2446, 2643
XspI CTAG 5 cut(s) 288, 489, 1761, 2795, 2988
ZrmI AGTACT 1 cut(s) 1327
Zsp2I ATGCAT 2 cut(s) 697, 3220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.