MD09G1261800.v1.1

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012264
Physical Location & Seq
Reverse (-)
33501464 .. 33515699
14236 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1261800.v1.1.491

Sequence Viewer

Length: 3279 bp
ATGTTAATGGATGGCACCATTTTAAAACTCCGCCTTGGGTCCTCAAAAGTCCAGGGAGAGTTGCCCCGAGAGATCACCTCTCGGACGAACCTGTCGAGTGACACCGGTGAGAATGGGTGGCTCATACGGTTCTTTGACTCAGCATTTTTCTGCGAATGGATTGCTGTTAGCTACCTGTACAAGCATGATCACGCGGGGGTGCGTGACTACCTCTGTAATCGAATGTACACTCTTCCCTTATCGGGTATCGAGAGCTACTTGTTTCAGATATGTTACATGTCAGTGCACAAGCCTAGCCCATCTTTGGATAAGTTTGTTATTGATATGTGCTCTAAGTCACTTAAGATAGCACTGAAGGTGCATTGGTTTTTATTGGCGGAGCTTGAAGACTCAGATGATAATGAAGGAATTAATAGGATTCAGGAGAAGTGTCAGATTGCAGCCACTTTGATGGGTGAGTGGCCACCTTTGGTACGTCCTCAAAATGAGTCAGCTAGTCCTGGGAGCAAGAACCAGGTATTGAATAGAATATTGTCATCAAAACAGAAATTGTTGTCACTAACGTCTTCACCACCTGCCCAGAGGTCTTTTTCTTTCTCACCTTCATCTGGAAACAATCTGCAAGAGGATGGTGCTCCGTTCTCTCCTGATGAGAACAAAATTTTTAAAAAATTCATTCCAGGGCCGAAGGTTCGGGATGCATTGCTCTTTAGAAAGTCAGTGGAGAAAGATGACGATGACACTGAAAAAGATGGGTTCTTTAAGAGGCTTTTAAGAGACAGTAGAGGTGATGATGAGACAGGTTCTAAGCTTAGAGATTCTTTGCTCTTTAGGAAGTCCTCAGAGAAGGATGATGATGCAGAGAAGGATGGCTTCTTTAAGAGGCTATTAAGGGATAGTAAAGGCGATGATGAGGAATTGACATCAAGCTCCGAGGGGTTTTTTAAGAGATTGTTTCGTGATAGTAAGAGTGATTCTGATGATAAATCAATCTCTAAATCAGTGGAAGATGGAGAAAAAGAAGGTTTCTTTCGGAAGTTTTTCAAAGATAAGTCTGAGGACAAGAAGGACCGGATTGATAGGAATGAGGATGAAGACGCACCATACTCTGAAGAAAAAGGTTCGAAATCTGCTGAAGATGATGAAAAAGAGGGGTTCTTCAGAAAATTCTTCAGGGATAAGTTTGACGACAAGAAAGATGGGAATGATAAAACTGAGGAAGGGAGTGCCAATGGAGATGAAGAAGAGCCTTCTGACTTTTCATTGTTTCGAAGATTGTTCCGTGTGCATCCTGAAGATGCGAAAAGTATTGCTGCAAATGAAAACAGCAGTACTGGTGGCTTGCCAGAAAGTAGTCCAGGGACTGAGAATTTTTTTCGCAAGTTGTTTAGGGATCGTGACCGTTCAGTTGAAGACTCAGAGCTATTTGGTTCAAGAAAACACAAAGAGAAACGCCCTGGTTCACCAAAGCAGCAAAATGAAAAGTCAAGTGTGAAACCCCCACTTCCAAATGGTACAACATCCCAATACCGTAAAGGGGCTTATCATGAGTCGCTGGACTTTGTGCAGTCACTATGTGAGACGTCTTATGGCTTAGTGGATATATTTCCAATTGAGGATCGCAAGAGTGCTCTTCGTGAGTCACTTGCGGAGATCAATTTGCACATTGCTGAAGCTCAAAATAGTGGAGGAGTTTGCTTTCCAATGGGGAAGGGGATGTATCGCGTGGTTTATATTCCAGAAGATGAAGCTGTTCTTTTGAATTCTAGGGAAAAGGCACCGTATCTCATATGTGTTGAAGTCCTGAAAAGTGAAATTACAGGCAATGCAAAGGACAAATCTGGTTCTCAACAGCTTTCTCGAGGGGGAATTCCTTTAGCAAATGGGGATGCACTTCTGCAAAGACCGCCTCCGTGGGCATATCCTTTGTGGACTGTCCAGGAGGTCTATCGCAATAGTAATGATAGGATGTCGAGTTCAACTGCTCAAGCAATTGACCAGGCAATGTCACATACGTCTGATGCGAAAGCGACATTTGTTACAGTAAAAATATCTGTGGAGAATAAATTGCATAGTCATACAGTAAAAGCAGAGAATATATCAAGGAATTATCAACGTAGAGAGGCTGCGACTTGTGCATCAAAAGAAGCACAAGAGAGTGATTTGGAGTGGGTGAGGGTGGTGCTTACAGCAGATCCTGGTGTTAGGATGGAGGACATTGAGGATCAAGGCCCCCCTCGGCGGAAGGAACACCGTCGTGTTCCAAGTACAGTGGCTATAGAGGAAGTAAAGGCTGCTGCAGCAAAAGGAGAAGCACCTCCTGGACTTCCTCTGAAAGGGGCTGGTCAGGAATCATCAGATGCACAGCCAATGGCTAATGGTGGTACTCCCAAAGCCAGCAATGCCTTGTCTGGCGAACTTTGGGAGGTAAAGAAGGAGAGATTAAGAAAAGCTTCAGTTCATGGAAAGTTACCTGGTTGGGACTTGCACTCAGTTATCGTGAAGAGTGGTGATGATTGTAGGCAGGAGCATCTTGCTGTGCAACTTATTTCTCACTTTTATGATATATTCCAAGAAGCTGGTCTTCCTCTCTGGTTGCGCCCTTATGAAATCTTAGTTACTTCTTCTTACACAGCTCTCATTGAAACAATTCCAGATACGGCTTCAATTCATTCTATCAAAAGTAGATATCCGAACATCATGAGCTTGCGAGACTTCTTTGCTGCTAAGTACCAAGAAAATTCTCCAAGTTTTAAACTTGCTCAGAGGAATTTTGTTGAAAGTATGGCTGGGTATTCTCTAGTGTGTTACCTTCTGCAGATAAAGGATAGGCACAATGGGAACCTCTTAATGGATGAAGAAGGTCATATTATACATATTGATTTTGGTTTCATGCTCTCCAATTCGCCTGGGGGTGTAAATTTTGAGAGTGCTCCATTCAAATTAACACGTGAACTTCTTGAGGTCATGGATTCTGATGCTGAGGGAGTTCCTAGCGAGTTCTTTGATTATTTTAAGGTTTTGTGCATTCAAGGGTTCCTTACATGTCGGAAGCATGCAGAACGCATTATTCTTCTTGTTGAGATGTTGCAGGACTCTGGTTTTCCATGCTTTAAAGGTGGTGCACGAACAATACAGAATCTGCGGAAACGGTTTCACTTGAGTTTGACAGAAGAGCAATGCGTATCCTTGGTGCTTTCTTTGATCAGCAGCAGCTTGGACGCATGGCGAACAAGGCAGTATGATTATTATCAGAGGGTTTTGAACGGAATATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004430 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012506 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017016 GO:0017137 GO:0019637 GO:0019899 GO:0019900 GO:0019901 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0030258 GO:0030659 GO:0031090 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0035618 GO:0035619 GO:0040007 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043424 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044706 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0048015 GO:0048017 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051704 GO:0051716 GO:0052742 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0097708 GO:0099402 GO:0120025 GO:0120038 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
PI3_PI4_kinase

Protein Analysis

1093

Amino Acids

123.26

Weight (kDa)

5.78

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI4KB-PIK1_PIK PF21245 54 - 124 3.9e-12 PI4KB/PIK1, accessory (PIK) domain
PI3_PI4_kinase PF00454 831 - 1039 2.9e-38 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09350 AT5G64070 AT5G64070
fragaria_vesca FvH4_1g24240
malus_domestica MD09G1261800.v1.1 MD17G1256600.v1.1
prunus_persica Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1 Prupe.3G159200_v2.0.a1
pyrus_communis pycom09g17610 pycom17g25880
rosa_chinensis RchiOBHm_Chr2g0120731
rosa_laevigata RLG00000018575
rosa_multiflora Rmu_sc0000319.1_g000011
rosa_roxburghii Rroxscaffold_2G00123200
rosa_rugosa Rorug02G0230500
rosa_samantha Rh2BG298500 Rh2CG276200
rosa_wichuraiana Rw2G023030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 583
AasI GACNNNNNNGTC 1 cut(s) 91
AatII GACGTC 1 cut(s) 1586
Acc36I ACCTGC 1 cut(s) 583
AccB1I GGYRCC 2 cut(s) 14, 1777
AccII CGCG 2 cut(s) 194, 1725
AciI CCGC 7 cut(s) 31, 194, 377, 1649, 1907, 2242, 3145
AclWI GGATC 4 cut(s) 1401, 1626, 2189, 2232
AcoI YGGCCR 1 cut(s) 461
AcsI RAATTY 9 cut(s) 660, 671, 1166, 1369, 1762, 1869, 2740, 2770, 2920
AcuI CTGAAG 8 cut(s) 374, 1131, 1144, 1155, 1156, 1314, 1692, 2439
AcvI CACGTG 1 cut(s) 2951
AcyI GRCGYC 1 cut(s) 1583
AdeI CACNNNGTG 1 cut(s) 1577
AfaI GTAC 8 cut(s) 179, 227, 474, 1333, 1516, 2269, 2386, 2732
AfiI CCNNNNNNNGG 7 cut(s) 242, 304, 608, 1537, 2241, 2336, 2851
AflII CTTAAG 1 cut(s) 341
AflIII ACRYGT 3 cut(s) 276, 2948, 3044
AgeI ACCGGT 1 cut(s) 104
AleI CACNNNNGTG 1 cut(s) 2256
AloI GAACNNNNNNTCC 2 cut(s) 1960, 1992
Alw21I GWGCWC 6 cut(s) 288, 332, 637, 1633, 2935, 3127
Alw26I GTCTC 4 cut(s) 771, 791, 1574, 2706
Alw44I GTGCAC 2 cut(s) 284, 3123
AlwI GGATC 4 cut(s) 1401, 1626, 2189, 2232
AlwNI CAGNNNCTG 3 cut(s) 1364, 2198, 3142
Ama87I CYCGRG 2 cut(s) 66, 1860
AoxI GGCC 3 cut(s) 461, 683, 2230
ApaLI GTGCAC 2 cut(s) 284, 3123
ApoI RAATTY 9 cut(s) 660, 671, 1166, 1369, 1762, 1869, 2740, 2770, 2920
AseI ATTAAT 1 cut(s) 411
AsiGI ACCGGT 1 cut(s) 104
Asp700I GAANNNNTTC 4 cut(s) 1040, 1752, 2452, 2649
AspLEI GCGC 1 cut(s) 2601
AspS9I GGNCC 4 cut(s) 39, 683, 1069, 2231
AsuHPI GGTGA 9 cut(s) 67, 119, 467, 561, 591, 800, 1455, 2185, 2522
AsuII TTCGAA 2 cut(s) 1124, 1270
AvaI CYCGRG 2 cut(s) 66, 1860
AvaII GGWCC 2 cut(s) 39, 1069
BaeGI GKGCMC 2 cut(s) 288, 3127
BalI TGGCCA 1 cut(s) 463
BanI GGYRCC 2 cut(s) 14, 1777
BbrPI CACGTG 1 cut(s) 2951
BbsI GAAGAC 5 cut(s) 393, 558, 1101, 1419, 2576
Bbv12I GWGCWC 6 cut(s) 288, 332, 637, 1633, 2935, 3127
BbvCI CCTCAGC 1 cut(s) 2982
BccI CCATC 9 cut(s) 5, 307, 445, 623, 746, 863, 1004, 1193, 2203
BceAI ACGGC 1 cut(s) 2676
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciVI GTATCC 1 cut(s) 3196
BclI TGATCA 2 cut(s) 187, 3204
BcoDI GTCTC 4 cut(s) 771, 791, 1574, 2706
BfaI CTAG 5 cut(s) 294, 495, 1767, 2801, 2994
BfmI CTRYAG 3 cut(s) 2277, 2298, 2816
BfrI CTTAAG 1 cut(s) 341
BfuAI ACCTGC 1 cut(s) 583
BfuI GTATCC 1 cut(s) 3196
BmcAI AGTACT 1 cut(s) 1333
Bme18I GGWCC 2 cut(s) 39, 1069
BmeT110I CYCGRG 2 cut(s) 66, 1860
BmgT120I GGNCC 4 cut(s) 39, 683, 1069, 2231
BmiI GGNNCC 6 cut(s) 16, 40, 1779, 2233, 2843, 3038
BpiI GAAGAC 5 cut(s) 393, 558, 1101, 1419, 2576
BplI GAGNNNNNCTC 2 cut(s) 62, 94
Bpu10I CCTNAGC 1 cut(s) 2982
Bpu14I TTCGAA 2 cut(s) 1124, 1270
BpuEI CTTGAG 3 cut(s) 1971, 2981, 3181
BsaAI YACGTR 1 cut(s) 2951
BsaBI GATNNNNATC 1 cut(s) 3249
BsaHI GRCGYC 1 cut(s) 1583
BsaWI WCCGGW 2 cut(s) 104, 1071
Bsc4I CCNNNNNNNGG 7 cut(s) 242, 304, 608, 1537, 2241, 2336, 2851
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 1 cut(s) 1339
Bse3DI GCAATG 6 cut(s) 701, 1665, 1831, 2010, 2407, 3185
Bse8I GATNNNNATC 1 cut(s) 3249
BseJI GATNNNNATC 1 cut(s) 3249
BseLI CCNNNNNNNGG 7 cut(s) 242, 304, 608, 1537, 2241, 2336, 2851
BseMI GCAATG 6 cut(s) 701, 1665, 1831, 2010, 2407, 3185
BseNI ACTGG 1 cut(s) 1339
BseRI GAGGAG 1 cut(s) 1704
BseSI GKGCMC 2 cut(s) 288, 3127
BseYI CCCAGC 1 cut(s) 2789
BsgI GTGCAG 1 cut(s) 1586
Bsh1236I CGCG 2 cut(s) 194, 1725
BshFI GGCC 3 cut(s) 463, 685, 2232
BshNI GGYRCC 2 cut(s) 14, 1777
BshTI ACCGGT 1 cut(s) 104
BsiHKAI GWGCWC 6 cut(s) 288, 332, 637, 1633, 2935, 3127
BsiHKCI CYCGRG 2 cut(s) 66, 1860
BsiSI CCGG 2 cut(s) 105, 1072
BslFI GGGAC 2 cut(s) 1375, 2495
BslI CCNNNNNNNGG 7 cut(s) 242, 304, 608, 1537, 2241, 2336, 2851
BsmAI GTCTC 4 cut(s) 771, 791, 1574, 2706
BsmBI CGTCTC 1 cut(s) 1574
BsmFI GGGAC 2 cut(s) 1375, 2495
BsmI GAATGC 1 cut(s) 3027
BsnI GGCC 3 cut(s) 463, 685, 2232
BsoBI CYCGRG 2 cut(s) 66, 1860
Bsp119I TTCGAA 2 cut(s) 1124, 1270
Bsp1286I GDGCHC 6 cut(s) 288, 332, 637, 1633, 2935, 3127
Bsp1407I TGTACA 2 cut(s) 177, 225
Bsp143I GATC 8 cut(s) 72, 187, 1393, 1618, 1653, 2194, 2224, 3204
BspACI CCGC 7 cut(s) 31, 194, 377, 1649, 1907, 2242, 3145
BspANI GGCC 3 cut(s) 463, 685, 2232
BspFNI CGCG 2 cut(s) 194, 1725
BspHI TCATGA 2 cut(s) 1546, 2700
BspLI GGNNCC 6 cut(s) 16, 40, 1779, 2233, 2843, 3038
BspMAI CTGCAG 2 cut(s) 2302, 2820
BspMI ACCTGC 1 cut(s) 583
BspPI GGATC 4 cut(s) 1401, 1626, 2189, 2232
BspQI GCTCTTC 3 cut(s) 1239, 1638, 3168
BspT104I TTCGAA 2 cut(s) 1124, 1270
BspT107I GGYRCC 2 cut(s) 14, 1777
BspTI CTTAAG 1 cut(s) 341
BsrDI GCAATG 6 cut(s) 701, 1665, 1831, 2010, 2407, 3185
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 2 cut(s) 177, 225
BsrI ACTGG 1 cut(s) 1339
BssAI RCCGGY 1 cut(s) 104
BssMI GATC 8 cut(s) 72, 187, 1393, 1618, 1653, 2194, 2224, 3204
BssNI GRCGYC 1 cut(s) 1583
BssT1I CCWWGG 2 cut(s) 34, 3189
Bst6I CTCTTC 5 cut(s) 237, 1239, 1638, 2498, 3168
BstACI GRCGYC 1 cut(s) 1583
BstAFI CTTAAG 1 cut(s) 341
BstAUI TGTACA 2 cut(s) 177, 225
BstBAI YACGTR 1 cut(s) 2951
BstBI TTCGAA 2 cut(s) 1124, 1270
BstC8I GCNNGC 4 cut(s) 1343, 2398, 2708, 3057
BstDSI CCRYGG 1 cut(s) 1913
BstENI CCTNNNNNAGG 1 cut(s) 2334
BstFNI CGCG 2 cut(s) 194, 1725
BstHHI GCGC 1 cut(s) 2601
BstKTI GATC 8 cut(s) 75, 190, 1396, 1621, 1656, 2197, 2227, 3207
BstMAI GTCTC 4 cut(s) 771, 791, 1574, 2706
BstMBI GATC 8 cut(s) 72, 187, 1393, 1618, 1653, 2194, 2224, 3204
BstMWI GCNNNNNNNGC 5 cut(s) 1906, 2135, 2300, 2402, 3235
BstNSI RCATGY 3 cut(s) 280, 3048, 3059
BstSFI CTRYAG 3 cut(s) 2277, 2298, 2816
BstSLI GKGCMC 2 cut(s) 288, 3127
BstUI CGCG 2 cut(s) 194, 1725
BstV2I GAAGAC 5 cut(s) 393, 558, 1101, 1419, 2576
BstX2I RGATCY 1 cut(s) 2194
BstXI CCANNNNNNTGG 2 cut(s) 451, 2579
BstYI RGATCY 1 cut(s) 2194
BsuI GTATCC 1 cut(s) 3196
BsuRI GGCC 3 cut(s) 463, 685, 2232
BtgI CCRYGG 1 cut(s) 1913
BtgZI GCGATG 1 cut(s) 921
BtsIMutI CAGTG 6 cut(s) 288, 350, 726, 741, 1008, 2277
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 4 cut(s) 1343, 2398, 2708, 3057
CaiI CAGNNNCTG 3 cut(s) 1364, 2198, 3142
CciI TCATGA 2 cut(s) 1546, 2700
CfoI GCGC 1 cut(s) 2601
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 4 cut(s) 39, 683, 1069, 2231
CseI GACGC 2 cut(s) 1106, 3230
CsiI ACCWGGT 2 cut(s) 513, 2473
Csp6I GTAC 8 cut(s) 178, 226, 473, 1332, 1515, 2268, 2385, 2731
CspAI ACCGGT 1 cut(s) 104
CspCI CAANNNNNGTGG 2 cut(s) 2253, 2288
CviQI GTAC 8 cut(s) 178, 226, 473, 1332, 1515, 2268, 2385, 2731
DpnI GATC 8 cut(s) 74, 189, 1395, 1620, 1655, 2196, 2226, 3206
DpnII GATC 8 cut(s) 72, 187, 1393, 1618, 1653, 2194, 2224, 3204
DraI TTTAAA 4 cut(s) 24, 667, 2755, 3115
DraIII CACNNNGTG 1 cut(s) 1577
DrdI GACNNNNNNGTC 1 cut(s) 91
DseDI GACNNNNNNGTC 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 461
Eam1104I CTCTTC 5 cut(s) 237, 1239, 1638, 2498, 3168
EarI CTCTTC 5 cut(s) 237, 1239, 1638, 2498, 3168
EciI GGCGGA 3 cut(s) 20, 392, 2257
Eco130I CCWWGG 2 cut(s) 34, 3189
Eco32I GATATC 1 cut(s) 2690
Eco47I GGWCC 2 cut(s) 39, 1069
Eco57I CTGAAG 8 cut(s) 374, 1131, 1144, 1155, 1156, 1314, 1692, 2439
Eco72I CACGTG 1 cut(s) 2951
Eco88I CYCGRG 2 cut(s) 66, 1860
EcoNI CCTNNNNNAGG 1 cut(s) 2334
EcoO109I RGGNCCY 2 cut(s) 39, 2231
EcoRI GAATTC 2 cut(s) 1762, 1869
EcoRV GATATC 1 cut(s) 2690
EcoT14I CCWWGG 2 cut(s) 34, 3189
EcoT22I ATGCAT 1 cut(s) 703
ErhI CCWWGG 2 cut(s) 34, 3189
Esp3I CGTCTC 1 cut(s) 1574
FaqI GGGAC 2 cut(s) 1375, 2495
FauI CCCGC 1 cut(s) 187
FauNDI CATATG 1 cut(s) 1790
FbaI TGATCA 2 cut(s) 187, 3204
FspBI CTAG 5 cut(s) 294, 495, 1767, 2801, 2994
GlaI GCGC 1 cut(s) 2600
GsaI CCCAGC 1 cut(s) 2793
HaeIII GGCC 3 cut(s) 463, 685, 2232
HapII CCGG 2 cut(s) 105, 1072
HgaI GACGC 2 cut(s) 1106, 3230
HhaI GCGC 1 cut(s) 2601
Hin1I GRCGYC 1 cut(s) 1583
Hin6I GCGC 1 cut(s) 2599
HinP1I GCGC 1 cut(s) 2599
HindIII AAGCTT 2 cut(s) 809, 2451
HpaII CCGG 2 cut(s) 105, 1072
HphI GGTGA 9 cut(s) 67, 119, 467, 561, 591, 800, 1455, 2185, 2522
Hpy166II GTNNAC 6 cut(s) 228, 286, 1463, 1932, 2954, 3125
Hpy8I GTNNAC 6 cut(s) 228, 286, 1463, 1932, 2954, 3125
Hpy99I CGWCG 1 cut(s) 2259
HpyCH4IV ACGT 6 cut(s) 475, 563, 1583, 2015, 2116, 2950
HpyF10VI GCNNNNNNNGC 5 cut(s) 1906, 2135, 2300, 2402, 3235
HpySE526I ACGT 6 cut(s) 475, 563, 1583, 2015, 2116, 2950
Hsp92I GRCGYC 1 cut(s) 1583
HspAI GCGC 1 cut(s) 2599
Ksp22I TGATCA 2 cut(s) 187, 3204
Kzo9I GATC 8 cut(s) 72, 187, 1393, 1618, 1653, 2194, 2224, 3204
LguI GCTCTTC 3 cut(s) 1239, 1638, 3168
LmnI GCTCC 6 cut(s) 379, 504, 640, 935, 2527, 2938
MabI ACCWGGT 2 cut(s) 513, 2473
MaeI CTAG 5 cut(s) 294, 495, 1767, 2801, 2994
MaeII ACGT 6 cut(s) 475, 563, 1583, 2015, 2116, 2950
MalI GATC 8 cut(s) 74, 189, 1395, 1620, 1655, 2196, 2226, 3206
MboI GATC 8 cut(s) 72, 187, 1393, 1618, 1653, 2194, 2224, 3204
MfeI CAATTG 2 cut(s) 1611, 1992
MflI RGATCY 1 cut(s) 2194
MhlI GDGCHC 6 cut(s) 288, 332, 637, 1633, 2935, 3127
MlsI TGGCCA 1 cut(s) 463
MluNI TGGCCA 1 cut(s) 463
MlyI GAGTC 7 cut(s) 131, 383, 497, 1409, 1559, 1649, 3089
MmeI TCCRAC 1 cut(s) 3029
Mox20I TGGCCA 1 cut(s) 463
Mph1103I ATGCAT 1 cut(s) 703
MroXI GAANNNNTTC 4 cut(s) 1040, 1752, 2452, 2649
MscI TGGCCA 1 cut(s) 463
MslI CAYNNNNRTG 4 cut(s) 281, 449, 2256, 2559
Msp20I TGGCCA 1 cut(s) 463
MspCI CTTAAG 1 cut(s) 341
MspI CCGG 2 cut(s) 105, 1072
MunI CAATTG 2 cut(s) 1611, 1992
Mva1269I GAATGC 1 cut(s) 3027
MvnI CGCG 2 cut(s) 194, 1725
MwoI GCNNNNNNNGC 5 cut(s) 1906, 2135, 2300, 2402, 3235
NdeI CATATG 1 cut(s) 1790
NdeII GATC 8 cut(s) 72, 187, 1393, 1618, 1653, 2194, 2224, 3204
NlaIV GGNNCC 6 cut(s) 16, 40, 1779, 2233, 2843, 3038
NmeAIII GCCGAG 1 cut(s) 2218
NmuCI GTSAC 8 cut(s) 98, 203, 336, 555, 1397, 1569, 1641, 2007
NsiI ATGCAT 1 cut(s) 703
NspI RCATGY 3 cut(s) 280, 3048, 3059
NspV TTCGAA 2 cut(s) 1124, 1270
OliI CACNNNNGTG 1 cut(s) 2256
PaeI GCATGC 1 cut(s) 3059
PaeR7I CTCGAG 1 cut(s) 1860
PagI TCATGA 2 cut(s) 1546, 2700
PaqCI CACCTGC 1 cut(s) 583
PciI ACATGT 2 cut(s) 276, 3044
PciSI GCTCTTC 3 cut(s) 1239, 1638, 3168
PcsI WCGNNNNNNNCGW 2 cut(s) 92, 2021
PctI GAATGC 1 cut(s) 3027
PdmI GAANNNNTTC 4 cut(s) 1040, 1752, 2452, 2649
PfeI GAWTC 6 cut(s) 418, 818, 974, 2351, 2972, 3139
PfoI TCCNGGA 2 cut(s) 1938, 2320
PinAI ACCGGT 1 cut(s) 104
PleI GAGTC 7 cut(s) 131, 383, 496, 1409, 1558, 1648, 3089
PmaCI CACGTG 1 cut(s) 2951
PmlI CACGTG 1 cut(s) 2951
PpsI GAGTC 7 cut(s) 131, 383, 496, 1409, 1558, 1648, 3089
Ppu21I YACGTR 1 cut(s) 2951
PpuMI RGGWCCY 1 cut(s) 39
PscI ACATGT 2 cut(s) 276, 3044
PshBI ATTAAT 1 cut(s) 411
Psp5II RGGWCCY 1 cut(s) 39
PspCI CACGTG 1 cut(s) 2951
PspFI CCCAGC 1 cut(s) 2789
PspN4I GGNNCC 6 cut(s) 16, 40, 1779, 2233, 2843, 3038
PspPI GGNCC 4 cut(s) 39, 683, 1069, 2231
PspPPI RGGWCCY 1 cut(s) 39
PstI CTGCAG 2 cut(s) 2302, 2820
PstNI CAGNNNCTG 3 cut(s) 1364, 2198, 3142
PsuI RGATCY 1 cut(s) 2194
RsaI GTAC 8 cut(s) 179, 227, 474, 1333, 1516, 2269, 2386, 2732
RsaNI GTAC 8 cut(s) 178, 226, 473, 1332, 1515, 2268, 2385, 2731
RseI CAYNNNNRTG 4 cut(s) 281, 449, 2256, 2559
SapI GCTCTTC 3 cut(s) 1239, 1638, 3168
Sau3AI GATC 8 cut(s) 72, 187, 1393, 1618, 1653, 2194, 2224, 3204
Sau96I GGNCC 4 cut(s) 39, 683, 1069, 2231
ScaI AGTACT 1 cut(s) 1333
SchI GAGTC 7 cut(s) 131, 383, 497, 1409, 1559, 1649, 3089
SduI GDGCHC 6 cut(s) 288, 332, 637, 1633, 2935, 3127
SexAI ACCWGGT 2 cut(s) 513, 2473
SfcI CTRYAG 3 cut(s) 2277, 2298, 2816
Sfr274I CTCGAG 1 cut(s) 1860
SfuI TTCGAA 2 cut(s) 1124, 1270
SgrAI CRCCGGYG 1 cut(s) 104
SinI GGWCC 2 cut(s) 39, 1069
SlaI CTCGAG 1 cut(s) 1860
SmiMI CAYNNNNRTG 4 cut(s) 281, 449, 2256, 2559
SmlI CTYRAG 5 cut(s) 341, 1860, 1986, 2960, 3160
SmoI CTYRAG 5 cut(s) 341, 1860, 1986, 2960, 3160
SphI GCATGC 1 cut(s) 3059
SsiI CCGC 7 cut(s) 31, 194, 377, 1649, 1907, 2242, 3145
SspI AATATT 2 cut(s) 531, 3273
SspMI CTAG 5 cut(s) 294, 495, 1767, 2801, 2994
StyI CCWWGG 2 cut(s) 34, 3189
TaiI ACGT 6 cut(s) 478, 566, 1586, 2018, 2119, 2953
TaqI TCGA 7 cut(s) 95, 220, 249, 1124, 1270, 1861, 1973
TatI WGTACW 4 cut(s) 177, 225, 1331, 2267
TfiI GAWTC 6 cut(s) 418, 818, 974, 2351, 2972, 3139
TscAI CASTG 6 cut(s) 288, 357, 726, 748, 1008, 2277
TseFI GTSAC 8 cut(s) 98, 203, 336, 555, 1397, 1569, 1641, 2007
Tsp45I GTSAC 8 cut(s) 98, 203, 336, 555, 1397, 1569, 1641, 2007
TspGWI ACGGA 3 cut(s) 627, 1271, 1902
TspRI CASTG 6 cut(s) 288, 357, 726, 748, 1008, 2277
Vha464I CTTAAG 1 cut(s) 341
VneI GTGCAC 2 cut(s) 284, 3123
VpaK11BI GGWCC 2 cut(s) 39, 1069
VspI ATTAAT 1 cut(s) 411
XagI CCTNNNNNAGG 1 cut(s) 2334
XapI RAATTY 9 cut(s) 660, 671, 1166, 1369, 1762, 1869, 2740, 2770, 2920
XceI RCATGY 3 cut(s) 280, 3048, 3059
XhoI CTCGAG 1 cut(s) 1860
XmnI GAANNNNTTC 4 cut(s) 1040, 1752, 2452, 2649
XspI CTAG 5 cut(s) 294, 495, 1767, 2801, 2994
ZraI GACGTC 1 cut(s) 1584
ZrmI AGTACT 1 cut(s) 1333
Zsp2I ATGCAT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.