AT5G64250

Nitronate monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013392
Physical Location & Seq
Reverse (-)
25697354 .. 25699255
1902 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64250.2

Sequence Viewer

Length: 882 bp
ATGAATCAGGAGTGTCCTGATTACTTGAGAGAGCTTATAAGAAAGACAAAGACATTGACAGAGAAACCATTTGGGATCGGTGTTGTTCTTGCGTTTCCTCATGATTTGAACATTAAGACAATCTTAGAAGAGAAAGTGGCTGTCTTGCAGCTTTATTGGGGTGATTGTTCACAGGAGCTTGTGGATGATGCTCACTTTGCCGGTGTCAAAGTTATTCCTCAGGTGGGGAATGTCGAAGAAGCTAGAAAGGCTGTTGCTGTAGGAGTAGACGCCATTATTGTCCAAGGGCGTGAAGCAGGTGGGCATGTTATTGGCAAGGATGGTCTCTTTTCATTGTTGCCAAGAGTGGTGGATTTAGTTGGGGAACGCGATATTCCCGTTATTGCTGCTGGAGGAATTGTGGATGGACGCGGTTATGTTGCAGCTTTGTCACTTGGGGCTCAAGGGGTCTGTCTAGGCACAAGATTTGTGGCGACTCATGAAAGCTATGCACACCCAATATACAAAAGGAAGTTGATTGAATATGAAAAAACCGAATACACGGATGTATTTGGAAGAGCAAGGTGGCCTGGTGCGCCTCAACGTGTTCTGGAGACGCCTTTCTTTGATGACTGGAGGTCTCTTCCGGCTCATGAGAATGAAGTTAACCAACCTATTATTGGACGTTCCACCATACATGGCGTTGAAAAGGAGATAAGAAGATTTTCAGGAACAGTACCAAACATGACGACAACAGGCGACTTAGAGAGTATGGCTATGTATGCAGGCCAAAGCGTAGGCCTTATCAAGGAGATTTTACCGGCTGGAGAGGTAGTGAAAAGTCTTGTGGAAGAAGCTCAGGCTCTGATCCTCCAAAAGTTCAACAATGCTACTACTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NMO

Protein Analysis

293

Amino Acids

32.09

Weight (kDa)

5.58

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NMO PF03060 2 - 71 9e-12 Nitronate monooxygenase
NMO PF03060 74 - 318 2.1e-46 Nitronate monooxygenase
FMN_dh PF01070 115 - 197 3.6e-09 FMN-dependent dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64250 AT5G64250
fragaria_vesca FvH4_5g07040 FvH4_5g07040
malus_domestica MD05G1043200.v1.1
prunus_persica Prupe.8G064000_v2.0.a1
pyrus_communis pycom05g03570
rosa_chinensis RchiOBHm_Chr6g0271551 RchiOBHm_Chr6g0271711
rosa_laevigata RLG00000013721 RLG00000013734
rosa_multiflora Rmu_sc0002396.1_g000003
rosa_roxburghii Rroxscaffold_7G00196850
rosa_rugosa Rorug06G0061800
rosa_samantha Rh6AG180600 Rh6BG186800 Rh6DG172300
rosa_wichuraiana Rw6G015510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 38
AarI CACCTGC 1 cut(s) 287
Acc36I ACCTGC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 267
AccII CGCG 2 cut(s) 369, 411
AciI CCGC 1 cut(s) 411
AclWI GGATC 2 cut(s) 83, 841
AcyI GRCGYC 2 cut(s) 270, 596
AfaI GTAC 1 cut(s) 717
AfiI CCNNNNNNNGG 3 cut(s) 224, 659, 787
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 4 cut(s) 109, 521, 686, 862
AhdI GACNNNNNGTC 1 cut(s) 616
AjnI CCWGG 1 cut(s) 568
AluBI AGCT 7 cut(s) 34, 151, 178, 242, 425, 486, 836
AluI AGCT 7 cut(s) 34, 151, 178, 242, 425, 486, 836
Alw26I GTCTC 3 cut(s) 329, 587, 624
AlwI GGATC 2 cut(s) 83, 841
AlwNI CAGNNNCTG 1 cut(s) 844
AoxI GGCC 3 cut(s) 566, 766, 778
ApeKI GCWGC 3 cut(s) 148, 386, 422
Asp700I GAANNNNTTC 1 cut(s) 703
AspLEI GCGC 1 cut(s) 577
AsuHPI GGTGA 1 cut(s) 173
AxyI CCTNAGG 1 cut(s) 219
BanII GRGCYC 1 cut(s) 442
BbvI GCAGC 3 cut(s) 160, 373, 434
BccI CCATC 2 cut(s) 314, 398
BciT130I CCWGG 1 cut(s) 570
BcoDI GTCTC 3 cut(s) 329, 587, 624
BfaI CTAG 2 cut(s) 243, 455
BfmI CTRYAG 1 cut(s) 258
BfuAI ACCTGC 1 cut(s) 287
BisI GCNGC 3 cut(s) 149, 387, 423
BlsI GCNGC 3 cut(s) 150, 388, 424
Bme1390I CCNGG 1 cut(s) 570
BmeRI GACNNNNNGTC 1 cut(s) 616
BmrFI CCNGG 1 cut(s) 570
BmsI GCATC 1 cut(s) 178
BpmI CTGGAG 4 cut(s) 411, 611, 634, 825
Bpu10I CCTNAGC 1 cut(s) 837
BpuEI CTTGAG 2 cut(s) 46, 426
BsaHI GRCGYC 2 cut(s) 270, 596
BsaI GGTCTC 2 cut(s) 329, 624
BsaJI CCNNGG 1 cut(s) 283
BsaXI ACNNNNNCTCC 4 cut(s) 384, 414, 798, 828
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 659, 787
Bse118I RCCGGY 2 cut(s) 200, 799
Bse1I ACTGG 1 cut(s) 617
Bse21I CCTNAGG 1 cut(s) 219
BseBI CCWGG 1 cut(s) 570
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 4 cut(s) 190, 325, 409, 550
BseLI CCNNNNNNNGG 3 cut(s) 224, 659, 787
BseMII CTCAG 2 cut(s) 233, 851
BseNI ACTGG 1 cut(s) 617
BseXI GCAGC 3 cut(s) 160, 373, 434
Bsh1236I CGCG 2 cut(s) 369, 411
BshFI GGCC 3 cut(s) 568, 768, 780
BsiSI CCGG 3 cut(s) 201, 626, 800
BslI CCNNNNNNNGG 3 cut(s) 224, 659, 787
BsmAI GTCTC 3 cut(s) 329, 587, 624
BsmBI CGTCTC 1 cut(s) 587
BsnI GGCC 3 cut(s) 568, 768, 780
Bso31I GGTCTC 2 cut(s) 329, 624
Bsp1286I GDGCHC 1 cut(s) 442
Bsp143I GATC 2 cut(s) 75, 846
BspACI CCGC 1 cut(s) 411
BspANI GGCC 3 cut(s) 568, 768, 780
BspCNI CTCAG 2 cut(s) 232, 850
BspFNI CGCG 2 cut(s) 369, 411
BspHI TCATGA 3 cut(s) 100, 478, 631
BspMI ACCTGC 1 cut(s) 287
BspPI GGATC 2 cut(s) 83, 841
BspQI GCTCTTC 1 cut(s) 550
BspTNI GGTCTC 2 cut(s) 329, 624
BsrFI RCCGGY 2 cut(s) 200, 799
BsrI ACTGG 1 cut(s) 617
BssAI RCCGGY 2 cut(s) 200, 799
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 2 cut(s) 75, 846
BssNI GRCGYC 2 cut(s) 270, 596
BssT1I CCWWGG 1 cut(s) 283
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 1 cut(s) 715
Bst6I CTCTTC 3 cut(s) 123, 550, 627
BstACI GRCGYC 2 cut(s) 270, 596
BstC8I GCNNGC 1 cut(s) 766
BstDEI CTNAG 4 cut(s) 124, 219, 742, 837
BstENI CCTNNNNNAGG 1 cut(s) 785
BstF5I GGATG 4 cut(s) 190, 325, 409, 550
BstFNI CGCG 2 cut(s) 369, 411
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 2 cut(s) 78, 849
BstMAI GTCTC 3 cut(s) 329, 587, 624
BstMBI GATC 2 cut(s) 75, 846
BstMWI GCNNNNNNNGC 4 cut(s) 197, 248, 574, 761
BstNI CCWGG 1 cut(s) 570
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 1 cut(s) 568
BstSFI CTRYAG 1 cut(s) 258
BstUI CGCG 2 cut(s) 369, 411
BstV1I GCAGC 3 cut(s) 160, 373, 434
Bsu36I CCTNAGG 1 cut(s) 219
BsuRI GGCC 3 cut(s) 568, 768, 780
BtsCI GGATG 4 cut(s) 190, 325, 409, 550
BveI ACCTGC 1 cut(s) 287
Cac8I GCNNGC 1 cut(s) 766
CaiI CAGNNNCTG 1 cut(s) 844
CciI TCATGA 3 cut(s) 100, 478, 631
CfoI GCGC 1 cut(s) 577
Cfr10I RCCGGY 2 cut(s) 200, 799
CseI GACGC 3 cut(s) 278, 417, 604
Csp6I GTAC 1 cut(s) 716
CspCI CAANNNNNGTGG 4 cut(s) 330, 365, 450, 485
CviAII CATG 7 cut(s) 101, 305, 479, 632, 677, 724, 879
CviQI GTAC 1 cut(s) 716
DdeI CTNAG 4 cut(s) 124, 219, 742, 837
DpnI GATC 2 cut(s) 77, 848
DpnII GATC 2 cut(s) 75, 846
DriI GACNNNNNGTC 1 cut(s) 616
Eam1104I CTCTTC 3 cut(s) 123, 550, 627
Eam1105I GACNNNNNGTC 1 cut(s) 616
EarI CTCTTC 3 cut(s) 123, 550, 627
Eco130I CCWWGG 1 cut(s) 283
Eco147I AGGCCT 1 cut(s) 780
Eco24I GRGCYC 1 cut(s) 442
Eco31I GGTCTC 2 cut(s) 329, 624
Eco81I CCTNAGG 1 cut(s) 219
EcoNI CCTNNNNNAGG 1 cut(s) 785
EcoRII CCWGG 1 cut(s) 568
EcoT14I CCWWGG 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 442
ErhI CCWWGG 1 cut(s) 283
Esp3I CGTCTC 1 cut(s) 587
FaeI CATG 7 cut(s) 104, 308, 482, 635, 680, 727, 882
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FatI CATG 7 cut(s) 100, 304, 478, 631, 676, 723, 878
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 3 cut(s) 149, 387, 423
FokI GGATG 4 cut(s) 197, 332, 416, 557
FriOI GRGCYC 1 cut(s) 442
Fsp4HI GCNGC 3 cut(s) 149, 387, 423
FspBI CTAG 2 cut(s) 243, 455
GlaI GCGC 1 cut(s) 576
GluI GCNGC 3 cut(s) 149, 387, 423
GsuI CTGGAG 4 cut(s) 411, 611, 634, 825
HaeIII GGCC 3 cut(s) 568, 768, 780
HapII CCGG 3 cut(s) 201, 626, 800
HgaI GACGC 3 cut(s) 278, 417, 604
HhaI GCGC 1 cut(s) 577
Hin1I GRCGYC 2 cut(s) 270, 596
Hin1II CATG 7 cut(s) 104, 308, 482, 635, 680, 727, 882
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 2 cut(s) 4, 475
HpaI GTTAAC 1 cut(s) 646
HpaII CCGG 3 cut(s) 201, 626, 800
HphI GGTGA 1 cut(s) 173
Hpy166II GTNNAC 3 cut(s) 170, 268, 646
Hpy188I TCNGA 1 cut(s) 846
Hpy188III TCNNGA 7 cut(s) 8, 17, 101, 479, 590, 632, 708
Hpy8I GTNNAC 3 cut(s) 170, 268, 646
HpyCH4III ACNGT 1 cut(s) 715
HpyCH4IV ACGT 2 cut(s) 583, 664
HpyCH4V TGCA 4 cut(s) 148, 422, 491, 764
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 248, 574, 761
HpyF3I CTNAG 4 cut(s) 124, 219, 742, 837
HpySE526I ACGT 2 cut(s) 583, 664
Hsp92I GRCGYC 2 cut(s) 270, 596
Hsp92II CATG 7 cut(s) 104, 308, 482, 635, 680, 727, 882
HspAI GCGC 1 cut(s) 575
KspAI GTTAAC 1 cut(s) 646
Kzo9I GATC 2 cut(s) 75, 846
LguI GCTCTTC 1 cut(s) 550
LmnI GCTCC 1 cut(s) 175
Lsp1109I GCAGC 3 cut(s) 160, 373, 434
LweI GCATC 1 cut(s) 178
MaeI CTAG 2 cut(s) 243, 455
MaeII ACGT 2 cut(s) 583, 664
MaeIII GTNAC 1 cut(s) 429
MalI GATC 2 cut(s) 77, 848
MboI GATC 2 cut(s) 75, 846
MboII GAAGA 6 cut(s) 140, 248, 567, 614, 711, 842
MhlI GDGCHC 1 cut(s) 442
MluCI AATT 1 cut(s) 396
MlyI GAGTC 1 cut(s) 469
MnlI CCTC 7 cut(s) 108, 228, 386, 588, 609, 802, 860
MroXI GAANNNNTTC 1 cut(s) 703
MseI TTAA 2 cut(s) 114, 645
MslI CAYNNNNRTG 1 cut(s) 636
MspI CCGG 3 cut(s) 201, 626, 800
MspR9I CCNGG 1 cut(s) 570
MvaI CCWGG 1 cut(s) 570
MvnI CGCG 2 cut(s) 369, 411
MwoI GCNNNNNNNGC 4 cut(s) 197, 248, 574, 761
NdeII GATC 2 cut(s) 75, 846
NlaIII CATG 7 cut(s) 104, 308, 482, 635, 680, 727, 882
NmuCI GTSAC 1 cut(s) 429
NspI RCATGY 1 cut(s) 308
PagI TCATGA 3 cut(s) 100, 478, 631
PaqCI CACCTGC 1 cut(s) 287
PceI AGGCCT 1 cut(s) 780
PciSI GCTCTTC 1 cut(s) 550
PdmI GAANNNNTTC 1 cut(s) 703
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 3 cut(s) 150, 388, 424
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
PsiI TTATAA 1 cut(s) 38
Psp6I CCWGG 1 cut(s) 568
PspGI CCWGG 1 cut(s) 568
PstNI CAGNNNCTG 1 cut(s) 844
RsaI GTAC 1 cut(s) 717
RsaNI GTAC 1 cut(s) 716
RseI CAYNNNNRTG 1 cut(s) 636
SapI GCTCTTC 1 cut(s) 550
SaqAI TTAA 2 cut(s) 114, 645
SatI GCNGC 3 cut(s) 149, 387, 423
Sau3AI GATC 2 cut(s) 75, 846
SchI GAGTC 1 cut(s) 469
ScrFI CCNGG 1 cut(s) 570
SduI GDGCHC 1 cut(s) 442
SfaNI GCATC 1 cut(s) 178
SfcI CTRYAG 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 636
SmlI CTYRAG 2 cut(s) 25, 441
SmoI CTYRAG 2 cut(s) 25, 441
Sse9I AATT 1 cut(s) 396
SseBI AGGCCT 1 cut(s) 780
SsiI CCGC 1 cut(s) 411
SspMI CTAG 2 cut(s) 243, 455
StuI AGGCCT 1 cut(s) 780
StyD4I CCNGG 1 cut(s) 568
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 1 cut(s) 715
TaiI ACGT 2 cut(s) 586, 667
TaqI TCGA 1 cut(s) 234
TasI AATT 1 cut(s) 396
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 114, 645
Tru9I TTAA 2 cut(s) 114, 645
TseFI GTSAC 1 cut(s) 429
TseI GCWGC 3 cut(s) 148, 386, 422
Tsp45I GTSAC 1 cut(s) 429
TspDTI ATGAA 5 cut(s) 17, 321, 495, 540, 654
TspGWI ACGGA 1 cut(s) 557
XagI CCTNNNNNAGG 1 cut(s) 785
XceI RCATGY 1 cut(s) 308
XcmI CCANNNNNNNNNTGG 1 cut(s) 656
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 703
XspI CTAG 2 cut(s) 243, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.