RchiOBHm_Chr6g0271711

nitronate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013392
Physical Location & Seq
Forward (+)
31594815 .. 31599441
4627 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24371

Sequence Viewer

Length: 996 bp
ATGGGTTGGCATGGGATTCTGGGTTTTGAGTATGGAATAGTTCAGGCACCATTGGGACCTGATATTTCAGGACCGGAGCTTGTTGCTGCTGTTGCTAATGCTGGTGGACTAGCTTTCCTCAGGGCTCCTGATTGGGAATCACCTGACTACCTCCGAGAGCTAATCAGTAAGACTCGAAGCTTAACTGACAAACCATTTGGGGTCGGTGTTGTCCTGGCATTTCCGCATGAGAAAAATATAAACGTCATCTTGGAGGAAAAGGTAGCAGTGCTACAGGTATACTGGGGTGATTGTTCTGTGGAGCTTGTGACGAAAGCTCATCGTTCTGGGGTTAAGATTGTTCCTCAAGTGAGTAGGGAGTTTGGAGGAGGCAAAAAAGCAATCGATGCTGGTGTAGATGCGATTATTGTCCAAGGACGTGAAGCAGGAGGGCATGTGATCAGTCAGGATGCTTTGATTTCATTGTTGCCAAAGGTAGTCGATCTTGTTGGAGATCGAAATATAATTGTCATTGCGGCAGGTGGTATTGTGGATGCTCGTGGATATGTTGCCGCCCTTGCCCTTGGTGCACAAGGAGTCTGCCTAGGCACTAGGTTTCTTGCAACTGAGGAAAGTCATGCTCATCCCACATACAAGAGGAAGTTGGTTGAATATGACAGAACTGAGTACACTGATATATTTGGTCGTGCAAGATGGCCCGGAGCACCCCATCGTGTTTTAAAGACGCCTTTCTTCAGTGATTGGAAGTCTCTTTCTGTTCATGAAACCGAAGCTAATCAGCCAGTCATTGGTCGAACAACAATACATGACAGGGAAATAGAAATCCGTCGTCTTGGCGGTACAGTGCCAAATGTGACGACAACTGGGGACATTGAAAGCATGGTTTTGTATGCTGGCCAAAGTGTAGGCCTCATCAAGGAAATTTTACCTGCAGCAGTGGTGGTAAAGAGGCTAGTTGAGGGTGCTCAACTTCTAATCAAGCAGAAGTTCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NMO

Protein Analysis

331

Amino Acids

35.75

Weight (kDa)

6.77

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NMO PF03060 6 - 73 3.3e-12 Nitronate monooxygenase
NMO PF03060 75 - 320 1.1e-37 Nitronate monooxygenase
FMN_dh PF01070 125 - 200 4.1e-06 FMN-dependent dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64250 AT5G64250
fragaria_vesca FvH4_5g07040 FvH4_5g07040
malus_domestica MD05G1043200.v1.1
prunus_persica Prupe.8G064000_v2.0.a1
pyrus_communis pycom05g03570
rosa_chinensis RchiOBHm_Chr6g0271551 RchiOBHm_Chr6g0271711
rosa_laevigata RLG00000013721 RLG00000013734
rosa_multiflora Rmu_sc0002396.1_g000003
rosa_roxburghii Rroxscaffold_7G00196850
rosa_rugosa Rorug06G0061800
rosa_samantha Rh6AG180600 Rh6BG186800 Rh6DG172300
rosa_wichuraiana Rw6G015510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 509
Acc36I ACCTGC 2 cut(s) 509, 937
AccB1I GGYRCC 1 cut(s) 46
AccB7I CCANNNNNTGG 1 cut(s) 788
AccI GTMKAC 1 cut(s) 279
AciI CCGC 4 cut(s) 224, 515, 552, 837
AcoI YGGCCR 1 cut(s) 895
AcsI RAATTY 1 cut(s) 921
AcuI CTGAAG 1 cut(s) 718
AcyI GRCGYC 1 cut(s) 725
AfaI GTAC 2 cut(s) 668, 841
AfiI CCNNNNNNNGG 2 cut(s) 788, 916
AgsI TTSAA 2 cut(s) 650, 875
AjiI CACGTC 1 cut(s) 419
AjnI CCWGG 1 cut(s) 213
AluBI AGCT 7 cut(s) 79, 113, 160, 180, 304, 317, 773
AluI AGCT 7 cut(s) 79, 113, 160, 180, 304, 317, 773
Alw21I GWGCWC 3 cut(s) 571, 706, 967
Alw26I GTCTC 1 cut(s) 753
Alw44I GTGCAC 1 cut(s) 567
AoxI GGCC 3 cut(s) 695, 895, 907
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 2 cut(s) 86, 932
ApoI RAATTY 1 cut(s) 921
ArsI GACNNNNNNTTYG 2 cut(s) 179, 211
AspA2I CCTAGG 1 cut(s) 583
AspS9I GGNCC 3 cut(s) 56, 71, 696
AsuC2I CCSGG 1 cut(s) 699
AsuHPI GGTGA 2 cut(s) 132, 299
AvaII GGWCC 2 cut(s) 56, 71
AvrII CCTAGG 1 cut(s) 583
AxyI CCTNAGG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 571
BalI TGGCCA 1 cut(s) 897
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 127
BauI CACGAG 1 cut(s) 537
Bbv12I GWGCWC 3 cut(s) 571, 706, 967
BbvI GCAGC 2 cut(s) 73, 944
BccI CCATC 2 cut(s) 687, 717
BciT130I CCWGG 1 cut(s) 215
BclI TGATCA 1 cut(s) 438
BcnI CCSGG 1 cut(s) 699
BcoDI GTCTC 1 cut(s) 753
BfaI CTAG 4 cut(s) 110, 584, 591, 953
BfmI CTRYAG 2 cut(s) 272, 930
BfuAI ACCTGC 2 cut(s) 509, 937
BisI GCNGC 4 cut(s) 87, 516, 552, 933
BlnI CCTAGG 1 cut(s) 583
BlsI GCNGC 4 cut(s) 88, 517, 553, 934
Bme1390I CCNGG 2 cut(s) 215, 699
Bme18I GGWCC 2 cut(s) 56, 71
BmgBI CACGTC 1 cut(s) 419
BmgT120I GGNCC 3 cut(s) 56, 71, 696
BmiI GGNNCC 3 cut(s) 48, 57, 126
BmrFI CCNGG 2 cut(s) 215, 699
BmrI ACTGGG 2 cut(s) 292, 873
BmsI GCATC 4 cut(s) 376, 388, 439, 523
BmuI ACTGGG 2 cut(s) 292, 873
BpuEI CTTGAG 1 cut(s) 330
BpuMI CCSGG 1 cut(s) 699
Bsa29I ATCGAT 1 cut(s) 384
BsaHI GRCGYC 1 cut(s) 725
BsaJI CCNNGG 3 cut(s) 412, 562, 583
BsaWI WCCGGW 1 cut(s) 73
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 2 cut(s) 788, 916
Bse1I ACTGG 3 cut(s) 287, 782, 868
Bse21I CCTNAGG 1 cut(s) 119
Bse3DI GCAATG 1 cut(s) 510
BseBI CCWGG 1 cut(s) 215
BseCI ATCGAT 1 cut(s) 384
BseDI CCNNGG 3 cut(s) 412, 562, 583
BseGI GGATG 3 cut(s) 454, 538, 622
BseLI CCNNNNNNNGG 2 cut(s) 788, 916
BseMI GCAATG 1 cut(s) 510
BseMII CTCAG 3 cut(s) 133, 597, 654
BseNI ACTGG 3 cut(s) 287, 782, 868
BseRI GAGGAG 1 cut(s) 381
BseSI GKGCMC 1 cut(s) 571
BseXI GCAGC 2 cut(s) 73, 944
BshFI GGCC 3 cut(s) 697, 897, 909
BshNI GGYRCC 1 cut(s) 46
BshVI ATCGAT 1 cut(s) 384
BsiHKAI GWGCWC 3 cut(s) 571, 706, 967
BsiSI CCGG 2 cut(s) 74, 699
BslFI GGGAC 2 cut(s) 69, 881
BslI CCNNNNNNNGG 2 cut(s) 788, 916
BsmAI GTCTC 1 cut(s) 753
BsmFI GGGAC 2 cut(s) 69, 881
BsnI GGCC 3 cut(s) 697, 897, 909
Bsp1286I GDGCHC 4 cut(s) 127, 571, 706, 967
Bsp143I GATC 3 cut(s) 438, 481, 493
BspACI CCGC 4 cut(s) 224, 515, 552, 837
BspANI GGCC 3 cut(s) 697, 897, 909
BspCNI CTCAG 3 cut(s) 132, 598, 655
BspDI ATCGAT 1 cut(s) 384
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 3 cut(s) 48, 57, 126
BspMAI CTGCAG 1 cut(s) 934
BspMI ACCTGC 2 cut(s) 509, 937
BspT107I GGYRCC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 510
BsrI ACTGG 3 cut(s) 287, 782, 868
BssECI CCNNGG 3 cut(s) 412, 562, 583
BssMI GATC 3 cut(s) 438, 481, 493
BssNAI GTATAC 1 cut(s) 280
BssNI GRCGYC 1 cut(s) 725
BssSI CACGAG 1 cut(s) 537
BssT1I CCWWGG 3 cut(s) 412, 562, 583
Bst1107I GTATAC 1 cut(s) 280
Bst2BI CACGAG 1 cut(s) 537
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 844
BstACI GRCGYC 1 cut(s) 725
BstAPI GCANNNNNTGC 1 cut(s) 386
BstC8I GCNNGC 1 cut(s) 895
BstDEI CTNAG 3 cut(s) 119, 606, 663
BstENI CCTNNNNNAGG 1 cut(s) 914
BstF5I GGATG 3 cut(s) 454, 538, 622
BstKTI GATC 3 cut(s) 441, 484, 496
BstMAI GTCTC 1 cut(s) 753
BstMBI GATC 3 cut(s) 438, 481, 493
BstMWI GCNNNNNNNGC 4 cut(s) 92, 386, 557, 566
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 1 cut(s) 437
BstSCI CCNGG 2 cut(s) 213, 697
BstSFI CTRYAG 2 cut(s) 272, 930
BstSLI GKGCMC 1 cut(s) 571
BstV1I GCAGC 2 cut(s) 73, 944
BstZ17I GTATAC 1 cut(s) 280
Bsu15I ATCGAT 1 cut(s) 384
Bsu36I CCTNAGG 1 cut(s) 119
BsuRI GGCC 3 cut(s) 697, 897, 909
BsuTUI ATCGAT 1 cut(s) 384
BtrI CACGTC 1 cut(s) 419
BtsCI GGATG 3 cut(s) 454, 538, 622
BtsI GCAGTG 2 cut(s) 273, 942
BtsIMutI CAGTG 5 cut(s) 273, 669, 742, 849, 942
BveI ACCTGC 2 cut(s) 509, 937
Cac8I GCNNGC 1 cut(s) 895
CciI TCATGA 1 cut(s) 760
Cfr13I GGNCC 3 cut(s) 56, 71, 696
ClaI ATCGAT 1 cut(s) 384
CseI GACGC 1 cut(s) 733
Csp6I GTAC 2 cut(s) 667, 840
CviAII CATG 7 cut(s) 11, 227, 434, 617, 761, 806, 880
CviQI GTAC 2 cut(s) 667, 840
DdeI CTNAG 3 cut(s) 119, 606, 663
DpnI GATC 3 cut(s) 440, 483, 495
DpnII GATC 3 cut(s) 438, 481, 493
DraI TTTAAA 1 cut(s) 720
EaeI YGGCCR 1 cut(s) 895
Eco130I CCWWGG 3 cut(s) 412, 562, 583
Eco147I AGGCCT 1 cut(s) 909
Eco24I GRGCYC 1 cut(s) 127
Eco47I GGWCC 2 cut(s) 56, 71
Eco57I CTGAAG 1 cut(s) 718
Eco81I CCTNAGG 1 cut(s) 119
EcoNI CCTNNNNNAGG 1 cut(s) 914
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 213
EcoT14I CCWWGG 3 cut(s) 412, 562, 583
EcoT38I GRGCYC 1 cut(s) 127
ErhI CCWWGG 3 cut(s) 412, 562, 583
FaeI CATG 7 cut(s) 14, 230, 437, 620, 764, 809, 883
FaqI GGGAC 2 cut(s) 69, 881
FatI CATG 7 cut(s) 10, 226, 433, 616, 760, 805, 879
FbaI TGATCA 1 cut(s) 438
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 4 cut(s) 87, 516, 552, 933
FokI GGATG 3 cut(s) 461, 545, 609
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 4 cut(s) 87, 516, 552, 933
FspBI CTAG 4 cut(s) 110, 584, 591, 953
GluI GCNGC 4 cut(s) 87, 516, 552, 933
HaeIII GGCC 3 cut(s) 697, 897, 909
HapII CCGG 2 cut(s) 74, 699
HgaI GACGC 1 cut(s) 733
Hin1I GRCGYC 1 cut(s) 725
Hin1II CATG 7 cut(s) 14, 230, 437, 620, 764, 809, 883
HindIII AAGCTT 1 cut(s) 178
HinfI GANTC 4 cut(s) 16, 137, 172, 576
HpaII CCGG 2 cut(s) 74, 699
HphI GGTGA 2 cut(s) 132, 299
Hpy166II GTNNAC 4 cut(s) 107, 280, 569, 669
Hpy188I TCNGA 1 cut(s) 155
Hpy188III TCNNGA 4 cut(s) 69, 128, 446, 761
Hpy8I GTNNAC 4 cut(s) 107, 280, 569, 669
Hpy99I CGWCG 1 cut(s) 831
HpyCH4III ACNGT 1 cut(s) 844
HpyCH4IV ACGT 2 cut(s) 243, 418
HpyCH4V TGCA 4 cut(s) 569, 602, 689, 932
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 386, 557, 566
HpyF3I CTNAG 3 cut(s) 119, 606, 663
HpySE526I ACGT 2 cut(s) 243, 418
Hsp92I GRCGYC 1 cut(s) 725
Hsp92II CATG 7 cut(s) 14, 230, 437, 620, 764, 809, 883
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 3 cut(s) 438, 481, 493
LmnI GCTCC 4 cut(s) 76, 130, 301, 701
Lsp1109I GCAGC 2 cut(s) 73, 944
LweI GCATC 4 cut(s) 376, 388, 439, 523
MaeI CTAG 4 cut(s) 110, 584, 591, 953
MaeII ACGT 2 cut(s) 243, 418
MaeIII GTNAC 2 cut(s) 307, 853
MalI GATC 3 cut(s) 440, 483, 495
MboI GATC 3 cut(s) 438, 481, 493
MboII GAAGA 1 cut(s) 724
MhlI GDGCHC 4 cut(s) 127, 571, 706, 967
MlsI TGGCCA 1 cut(s) 897
MluCI AATT 2 cut(s) 504, 921
MluNI TGGCCA 1 cut(s) 897
MlyI GAGTC 2 cut(s) 166, 585
MmeI TCCRAC 1 cut(s) 469
Mox20I TGGCCA 1 cut(s) 897
MscI TGGCCA 1 cut(s) 897
MseI TTAA 4 cut(s) 182, 333, 719, 994
Msp20I TGGCCA 1 cut(s) 897
MspI CCGG 2 cut(s) 74, 699
MspR9I CCNGG 2 cut(s) 215, 699
MvaI CCWGG 1 cut(s) 215
MwoI GCNNNNNNNGC 4 cut(s) 92, 386, 557, 566
NciI CCSGG 1 cut(s) 699
NdeII GATC 3 cut(s) 438, 481, 493
NlaIII CATG 7 cut(s) 14, 230, 437, 620, 764, 809, 883
NlaIV GGNNCC 3 cut(s) 48, 57, 126
NmuCI GTSAC 2 cut(s) 307, 853
NspI RCATGY 1 cut(s) 437
PagI TCATGA 1 cut(s) 760
PaqCI CACCTGC 1 cut(s) 509
PceI AGGCCT 1 cut(s) 909
PfeI GAWTC 2 cut(s) 16, 137
PflMI CCANNNNNTGG 1 cut(s) 788
PkrI GCNGC 4 cut(s) 88, 517, 553, 934
PleI GAGTC 2 cut(s) 166, 584
PpsI GAGTC 2 cut(s) 166, 584
PpuMI RGGWCCY 1 cut(s) 56
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 3 cut(s) 48, 57, 126
PspPI GGNCC 3 cut(s) 56, 71, 696
PspPPI RGGWCCY 1 cut(s) 56
PstI CTGCAG 1 cut(s) 934
RsaI GTAC 2 cut(s) 668, 841
RsaNI GTAC 2 cut(s) 667, 840
SaqAI TTAA 4 cut(s) 182, 333, 719, 994
SatI GCNGC 4 cut(s) 87, 516, 552, 933
Sau3AI GATC 3 cut(s) 438, 481, 493
Sau96I GGNCC 3 cut(s) 56, 71, 696
SchI GAGTC 2 cut(s) 166, 585
ScrFI CCNGG 2 cut(s) 215, 699
SduI GDGCHC 4 cut(s) 127, 571, 706, 967
SfaNI GCATC 4 cut(s) 376, 388, 439, 523
SfcI CTRYAG 2 cut(s) 272, 930
SinI GGWCC 2 cut(s) 56, 71
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
Sse9I AATT 2 cut(s) 504, 921
SseBI AGGCCT 1 cut(s) 909
SsiI CCGC 4 cut(s) 224, 515, 552, 837
SspMI CTAG 4 cut(s) 110, 584, 591, 953
StuI AGGCCT 1 cut(s) 909
StyD4I CCNGG 2 cut(s) 213, 697
StyI CCWWGG 3 cut(s) 412, 562, 583
TaaI ACNGT 1 cut(s) 844
TaiI ACGT 2 cut(s) 246, 421
TaqI TCGA 5 cut(s) 175, 384, 480, 496, 793
TasI AATT 2 cut(s) 504, 921
TatI WGTACW 1 cut(s) 666
TauI GCSGC 2 cut(s) 518, 554
TfiI GAWTC 2 cut(s) 16, 137
Tru1I TTAA 4 cut(s) 182, 333, 719, 994
Tru9I TTAA 4 cut(s) 182, 333, 719, 994
TscAI CASTG 5 cut(s) 273, 676, 742, 849, 942
TseFI GTSAC 2 cut(s) 307, 853
TseI GCWGC 2 cut(s) 86, 932
Tsp45I GTSAC 2 cut(s) 307, 853
TspDTI ATGAA 3 cut(s) 450, 749, 777
TspGWI ACGGA 1 cut(s) 815
TspRI CASTG 5 cut(s) 273, 676, 742, 849, 942
Van91I CCANNNNNTGG 1 cut(s) 788
VneI GTGCAC 1 cut(s) 567
VpaK11BI GGWCC 2 cut(s) 56, 71
XagI CCTNNNNNAGG 1 cut(s) 914
XapI RAATTY 1 cut(s) 921
XceI RCATGY 1 cut(s) 437
XmaJI CCTAGG 1 cut(s) 583
XmiI GTMKAC 1 cut(s) 279
XspI CTAG 4 cut(s) 110, 584, 591, 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.