RLG00000013734

nitronate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013392
Physical Location & Seq
Reverse (-)
37494283 .. 37498298
4016 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013734

Sequence Viewer

Length: 906 bp
ATGGGTTGGCATGGGATTCTGGGTTTTGAGTATGGAATAGTTCAGGCACCATTGGGACCTGATATTTCTGGACCGGAGCTTGTTGCTGCTGTTGCTAATGCTGGTGGACTAGCTTTCCTCAGGGCTCCTGATTGGAAAAATATAAACGTCATCTTGGAGGAAAAGGTAGCAGTGCTACAGGTATACTGGGGTGATTGTTCTGTGGAGCTTGTGATGAAAGCTCATCGTTCTGGGGTTAAGATTGTTCCTCAAGTAGGGAGTTTGGAGGAGGCAAAAAAAGCAATCGATGCTGGTGTAGATGCGATTATCGTCCAAGGACGTGAAGCAGGGGGTCATGTGATCAGTCAGGATGCTTTGATTTCATTGTTGCCAAAGGTAGTCGATCTTGTTGGAGATCGAAATATAACTGTCATTGCGGCAGGTGGTATTGTGGATGCTCGTGGATATGTTGCCGCCTTTGCCCTTGGTGCACAAGGAGTCTGCCTAGGCACTAGGTTTCTTGCAACCGAGGAAAGTTGTGCTCATCCCACATACAAGAGGAAGTTGGTTGAATATGACAGAACTGAGTACACTGATATATTTAGTCGTGCAAGATGGCCTGGAGCACCACATCGTGTTTTAAAGACGCCTTTCTTCAGTGATTGGAAGTCTCTTCCTGTTCATGAAACCGAAGCTAATCAGCCAGTCATTGGTCGAACAACAATACATGACAGGGAAATAGAAATCCGTCGTCTTGGCGGTACAGTGCCAAATGTGACGACAACTGGTGACATTGAAAGCATGGTTTTGTATGCTGGCCAAAGTGTAGGCCTCATCAAGGAAATTTTACCTGCAGCAGAGGTGGTAAAGAGGCTAGTTGAAGGCGCTCAACTTCTAATCAAGCAGAAGTTCAGTTATATGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NMO

Protein Analysis

302

Amino Acids

32.53

Weight (kDa)

6.19

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NMO PF03060 45 - 287 4.8e-44 Nitronate monooxygenase
FMN_dh PF01070 84 - 167 2.1e-09 FMN-dependent dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64250 AT5G64250
fragaria_vesca FvH4_5g07040 FvH4_5g07040
malus_domestica MD05G1043200.v1.1
prunus_persica Prupe.8G064000_v2.0.a1
pyrus_communis pycom05g03570
rosa_chinensis RchiOBHm_Chr6g0271551 RchiOBHm_Chr6g0271711
rosa_laevigata RLG00000013721 RLG00000013734
rosa_multiflora Rmu_sc0002396.1_g000003
rosa_roxburghii Rroxscaffold_7G00196850
rosa_rugosa Rorug06G0061800
rosa_samantha Rh6AG180600 Rh6BG186800 Rh6DG172300
rosa_wichuraiana Rw6G015510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 410
Acc36I ACCTGC 2 cut(s) 410, 838
AccB1I GGYRCC 1 cut(s) 46
AccB7I CCANNNNNTGG 1 cut(s) 689
AccI GTMKAC 1 cut(s) 183
AciI CCGC 3 cut(s) 416, 453, 738
AcoI YGGCCR 1 cut(s) 796
AcsI RAATTY 1 cut(s) 822
AcuI CTGAAG 1 cut(s) 619
AcyI GRCGYC 1 cut(s) 626
AdeI CACNNNGTG 1 cut(s) 614
AfaI GTAC 2 cut(s) 569, 742
AfiI CCNNNNNNNGG 3 cut(s) 254, 689, 817
AgsI TTSAA 3 cut(s) 551, 776, 860
AjiI CACGTC 1 cut(s) 320
AjnI CCWGG 1 cut(s) 598
AluBI AGCT 5 cut(s) 79, 113, 208, 221, 674
AluI AGCT 5 cut(s) 79, 113, 208, 221, 674
Alw21I GWGCWC 3 cut(s) 472, 523, 607
Alw26I GTCTC 1 cut(s) 654
Alw44I GTGCAC 1 cut(s) 468
AoxI GGCC 3 cut(s) 596, 796, 808
ApaLI GTGCAC 1 cut(s) 468
ApeKI GCWGC 2 cut(s) 86, 833
ApoI RAATTY 1 cut(s) 822
AspA2I CCTAGG 1 cut(s) 484
AspLEI GCGC 1 cut(s) 866
AspS9I GGNCC 2 cut(s) 56, 71
AsuHPI GGTGA 2 cut(s) 203, 779
AvaII GGWCC 2 cut(s) 56, 71
AvrII CCTAGG 1 cut(s) 484
AxyI CCTNAGG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 472
BalI TGGCCA 1 cut(s) 798
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 127
BauI CACGAG 1 cut(s) 438
Bbv12I GWGCWC 3 cut(s) 472, 523, 607
BbvI GCAGC 2 cut(s) 73, 845
BccI CCATC 1 cut(s) 588
BciT130I CCWGG 1 cut(s) 600
BclI TGATCA 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 654
BfaI CTAG 4 cut(s) 110, 485, 492, 854
BfmI CTRYAG 2 cut(s) 176, 831
BfoI RGCGCY 1 cut(s) 867
BfuAI ACCTGC 2 cut(s) 410, 838
BisI GCNGC 4 cut(s) 87, 417, 453, 834
BlnI CCTAGG 1 cut(s) 484
BlsI GCNGC 4 cut(s) 88, 418, 454, 835
Bme1390I CCNGG 1 cut(s) 600
Bme18I GGWCC 2 cut(s) 56, 71
BmgBI CACGTC 1 cut(s) 320
BmgT120I GGNCC 2 cut(s) 56, 71
BmiI GGNNCC 3 cut(s) 48, 57, 126
BmrFI CCNGG 1 cut(s) 600
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 4 cut(s) 277, 289, 340, 424
BmuI ACTGGG 1 cut(s) 196
BpmI CTGGAG 1 cut(s) 621
BpuEI CTTGAG 1 cut(s) 234
Bsa29I ATCGAT 1 cut(s) 285
BsaHI GRCGYC 1 cut(s) 626
BsaJI CCNNGG 4 cut(s) 313, 463, 484, 507
BsaWI WCCGGW 1 cut(s) 73
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 689, 817
Bse1I ACTGG 3 cut(s) 191, 683, 769
Bse21I CCTNAGG 1 cut(s) 119
Bse3DI GCAATG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 600
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 4 cut(s) 313, 463, 484, 507
BseGI GGATG 3 cut(s) 355, 439, 523
BseLI CCNNNNNNNGG 3 cut(s) 254, 689, 817
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 2 cut(s) 133, 555
BseNI ACTGG 3 cut(s) 191, 683, 769
BseRI GAGGAG 1 cut(s) 281
BseSI GKGCMC 1 cut(s) 472
BseXI GCAGC 2 cut(s) 73, 845
BshFI GGCC 3 cut(s) 598, 798, 810
BshNI GGYRCC 1 cut(s) 46
BshVI ATCGAT 1 cut(s) 285
BsiHKAI GWGCWC 3 cut(s) 472, 523, 607
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 3 cut(s) 254, 689, 817
BsmAI GTCTC 1 cut(s) 654
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 3 cut(s) 598, 798, 810
Bsp1286I GDGCHC 4 cut(s) 127, 472, 523, 607
Bsp143I GATC 3 cut(s) 339, 382, 394
BspACI CCGC 3 cut(s) 416, 453, 738
BspANI GGCC 3 cut(s) 598, 798, 810
BspCNI CTCAG 2 cut(s) 132, 556
BspDI ATCGAT 1 cut(s) 285
BspHI TCATGA 1 cut(s) 661
BspLI GGNNCC 3 cut(s) 48, 57, 126
BspMAI CTGCAG 1 cut(s) 835
BspMI ACCTGC 2 cut(s) 410, 838
BspT107I GGYRCC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 411
BsrI ACTGG 3 cut(s) 191, 683, 769
BssECI CCNNGG 4 cut(s) 313, 463, 484, 507
BssMI GATC 3 cut(s) 339, 382, 394
BssNAI GTATAC 1 cut(s) 184
BssNI GRCGYC 1 cut(s) 626
BssSI CACGAG 1 cut(s) 438
BssT1I CCWWGG 3 cut(s) 313, 463, 484
Bst1107I GTATAC 1 cut(s) 184
Bst2BI CACGAG 1 cut(s) 438
Bst2UI CCWGG 1 cut(s) 600
Bst4CI ACNGT 2 cut(s) 409, 745
Bst6I CTCTTC 1 cut(s) 657
BstACI GRCGYC 1 cut(s) 626
BstAPI GCANNNNNTGC 1 cut(s) 287
BstC8I GCNNGC 1 cut(s) 796
BstDEI CTNAG 2 cut(s) 119, 564
BstENI CCTNNNNNAGG 2 cut(s) 252, 815
BstF5I GGATG 3 cut(s) 355, 439, 523
BstH2I RGCGCY 1 cut(s) 867
BstHHI GCGC 1 cut(s) 866
BstKTI GATC 3 cut(s) 342, 385, 397
BstMAI GTCTC 1 cut(s) 654
BstMBI GATC 3 cut(s) 339, 382, 394
BstMWI GCNNNNNNNGC 5 cut(s) 92, 278, 287, 458, 467
BstNI CCWGG 1 cut(s) 600
BstSCI CCNGG 1 cut(s) 598
BstSFI CTRYAG 2 cut(s) 176, 831
BstSLI GKGCMC 1 cut(s) 472
BstV1I GCAGC 2 cut(s) 73, 845
BstZ17I GTATAC 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 285
Bsu36I CCTNAGG 1 cut(s) 119
BsuRI GGCC 3 cut(s) 598, 798, 810
BsuTUI ATCGAT 1 cut(s) 285
BtrI CACGTC 1 cut(s) 320
BtsCI GGATG 3 cut(s) 355, 439, 523
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 4 cut(s) 177, 570, 643, 750
BveI ACCTGC 2 cut(s) 410, 838
Cac8I GCNNGC 1 cut(s) 796
CciI TCATGA 1 cut(s) 661
CfoI GCGC 1 cut(s) 866
Cfr13I GGNCC 2 cut(s) 56, 71
ClaI ATCGAT 1 cut(s) 285
CseI GACGC 1 cut(s) 634
Csp6I GTAC 2 cut(s) 568, 741
CviAII CATG 5 cut(s) 11, 335, 662, 707, 781
CviQI GTAC 2 cut(s) 568, 741
DdeI CTNAG 2 cut(s) 119, 564
DpnI GATC 3 cut(s) 341, 384, 396
DpnII GATC 3 cut(s) 339, 382, 394
DraI TTTAAA 1 cut(s) 621
DraIII CACNNNGTG 1 cut(s) 614
EaeI YGGCCR 1 cut(s) 796
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
Eco130I CCWWGG 3 cut(s) 313, 463, 484
Eco147I AGGCCT 1 cut(s) 810
Eco24I GRGCYC 1 cut(s) 127
Eco47I GGWCC 2 cut(s) 56, 71
Eco57I CTGAAG 1 cut(s) 619
Eco81I CCTNAGG 1 cut(s) 119
EcoNI CCTNNNNNAGG 2 cut(s) 252, 815
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 598
EcoT14I CCWWGG 3 cut(s) 313, 463, 484
EcoT38I GRGCYC 1 cut(s) 127
ErhI CCWWGG 3 cut(s) 313, 463, 484
FaeI CATG 5 cut(s) 14, 338, 665, 710, 784
FaqI GGGAC 1 cut(s) 69
FatI CATG 5 cut(s) 10, 334, 661, 706, 780
FbaI TGATCA 1 cut(s) 339
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 4 cut(s) 87, 417, 453, 834
FokI GGATG 3 cut(s) 362, 446, 510
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 4 cut(s) 87, 417, 453, 834
FspBI CTAG 4 cut(s) 110, 485, 492, 854
GlaI GCGC 1 cut(s) 865
GluI GCNGC 4 cut(s) 87, 417, 453, 834
GsuI CTGGAG 1 cut(s) 621
HaeII RGCGCY 1 cut(s) 867
HaeIII GGCC 3 cut(s) 598, 798, 810
HapII CCGG 1 cut(s) 74
HgaI GACGC 1 cut(s) 634
HhaI GCGC 1 cut(s) 866
Hin1I GRCGYC 1 cut(s) 626
Hin1II CATG 5 cut(s) 14, 338, 665, 710, 784
Hin6I GCGC 1 cut(s) 864
HinP1I GCGC 1 cut(s) 864
HinfI GANTC 2 cut(s) 16, 477
HpaII CCGG 1 cut(s) 74
HphI GGTGA 2 cut(s) 203, 779
Hpy166II GTNNAC 4 cut(s) 107, 184, 470, 570
Hpy188III TCNNGA 4 cut(s) 69, 128, 347, 662
Hpy8I GTNNAC 4 cut(s) 107, 184, 470, 570
Hpy99I CGWCG 1 cut(s) 732
HpyAV CCTTC 1 cut(s) 854
HpyCH4III ACNGT 2 cut(s) 409, 745
HpyCH4IV ACGT 2 cut(s) 147, 319
HpyCH4V TGCA 4 cut(s) 470, 503, 590, 833
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 278, 287, 458, 467
HpyF3I CTNAG 2 cut(s) 119, 564
HpySE526I ACGT 2 cut(s) 147, 319
Hsp92I GRCGYC 1 cut(s) 626
Hsp92II CATG 5 cut(s) 14, 338, 665, 710, 784
HspAI GCGC 1 cut(s) 864
Ksp22I TGATCA 1 cut(s) 339
Kzo9I GATC 3 cut(s) 339, 382, 394
LmnI GCTCC 4 cut(s) 76, 130, 205, 602
Lsp1109I GCAGC 2 cut(s) 73, 845
LweI GCATC 4 cut(s) 277, 289, 340, 424
MaeI CTAG 4 cut(s) 110, 485, 492, 854
MaeII ACGT 2 cut(s) 147, 319
MaeIII GTNAC 2 cut(s) 754, 767
MalI GATC 3 cut(s) 341, 384, 396
MboI GATC 3 cut(s) 339, 382, 394
MboII GAAGA 2 cut(s) 625, 644
MhlI GDGCHC 4 cut(s) 127, 472, 523, 607
MlsI TGGCCA 1 cut(s) 798
MluCI AATT 1 cut(s) 822
MluNI TGGCCA 1 cut(s) 798
MlyI GAGTC 1 cut(s) 486
MmeI TCCRAC 1 cut(s) 370
Mox20I TGGCCA 1 cut(s) 798
MscI TGGCCA 1 cut(s) 798
MseI TTAA 2 cut(s) 237, 620
Msp20I TGGCCA 1 cut(s) 798
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 1 cut(s) 600
MvaI CCWGG 1 cut(s) 600
MwoI GCNNNNNNNGC 5 cut(s) 92, 278, 287, 458, 467
NdeII GATC 3 cut(s) 339, 382, 394
NlaIII CATG 5 cut(s) 14, 338, 665, 710, 784
NlaIV GGNNCC 3 cut(s) 48, 57, 126
NmuCI GTSAC 2 cut(s) 754, 767
PagI TCATGA 1 cut(s) 661
PaqCI CACCTGC 1 cut(s) 410
PceI AGGCCT 1 cut(s) 810
PfeI GAWTC 1 cut(s) 16
PflMI CCANNNNNTGG 1 cut(s) 689
PkrI GCNGC 4 cut(s) 88, 418, 454, 835
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
PpuMI RGGWCCY 1 cut(s) 56
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 598
PspGI CCWGG 1 cut(s) 598
PspN4I GGNNCC 3 cut(s) 48, 57, 126
PspPI GGNCC 2 cut(s) 56, 71
PspPPI RGGWCCY 1 cut(s) 56
PstI CTGCAG 1 cut(s) 835
RsaI GTAC 2 cut(s) 569, 742
RsaNI GTAC 2 cut(s) 568, 741
SaqAI TTAA 2 cut(s) 237, 620
SatI GCNGC 4 cut(s) 87, 417, 453, 834
Sau3AI GATC 3 cut(s) 339, 382, 394
Sau96I GGNCC 2 cut(s) 56, 71
SchI GAGTC 1 cut(s) 486
ScrFI CCNGG 1 cut(s) 600
SduI GDGCHC 4 cut(s) 127, 472, 523, 607
SfaNI GCATC 4 cut(s) 277, 289, 340, 424
SfcI CTRYAG 2 cut(s) 176, 831
SinI GGWCC 2 cut(s) 56, 71
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
Sse9I AATT 1 cut(s) 822
SseBI AGGCCT 1 cut(s) 810
SsiI CCGC 3 cut(s) 416, 453, 738
SspMI CTAG 4 cut(s) 110, 485, 492, 854
StuI AGGCCT 1 cut(s) 810
StyD4I CCNGG 1 cut(s) 598
StyI CCWWGG 3 cut(s) 313, 463, 484
TaaI ACNGT 2 cut(s) 409, 745
TaiI ACGT 2 cut(s) 150, 322
TaqI TCGA 4 cut(s) 285, 381, 397, 694
TasI AATT 1 cut(s) 822
TatI WGTACW 1 cut(s) 567
TauI GCSGC 2 cut(s) 419, 455
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 2 cut(s) 237, 620
Tru9I TTAA 2 cut(s) 237, 620
TscAI CASTG 4 cut(s) 177, 577, 643, 750
TseFI GTSAC 2 cut(s) 754, 767
TseI GCWGC 2 cut(s) 86, 833
Tsp45I GTSAC 2 cut(s) 754, 767
TspDTI ATGAA 4 cut(s) 230, 351, 650, 678
TspGWI ACGGA 1 cut(s) 716
TspRI CASTG 4 cut(s) 177, 577, 643, 750
Van91I CCANNNNNTGG 1 cut(s) 689
VneI GTGCAC 1 cut(s) 468
VpaK11BI GGWCC 2 cut(s) 56, 71
XagI CCTNNNNNAGG 2 cut(s) 252, 815
XapI RAATTY 1 cut(s) 822
XmaJI CCTAGG 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 4 cut(s) 110, 485, 492, 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.