RLG00000013721

nitronate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013392
Physical Location & Seq
Reverse (-)
36648349 .. 36658200
9852 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013721

Sequence Viewer

Length: 1002 bp
ATGGGTTGGCATGGGATTCTGGGTTTTGAGTATGGAATAGTTCAGGCACCATTGGGACCTGATATTTCTGGACCGGAGCTTGTTGCTGCCGTTGCTAATGCTGGTGGACTAGCTTTCCTCAGGGCTCCTGATTGGGAATCACCGGACTACCTCCGAGAGCTAATCAGTAAGACTCGAAGCTTAACTGACAAACCATTTGGGGTCGGTGTAGTCCTGGCATTTCCGCATGAGAAAAATATAAACGTCATTTTGGAGGAAAAGGTAGCAGTGCTACAGGTATACTGGGGTGATTGTTCTGTGGAGCTTGTGACGAAAGCTCATCGTTCTGGGGTTAAGATTGTTCCTCAAGTAGGGAGTTTGGAGGAGGCAAAAAAAGCAATCGATGCTGGTGTAGATGCGATTATCGTCCAAGGACGTGAAGCAGGAGGGCATGTGATCAGTCAGGATGCTTTGATTTCATTGTTGCCAAAGGTAGTCGATCTTGTTGGAGATCGAAATATAACTGTCATTGCAGCAGGTGGTATTGTGGATGCTCGTGGATATGTTGCCGCCCTTGCCCTTGGTGCACAAGGAGTCTGCCTAGGCACTAGGTTTCTTGCAACTGAGGAAAGTTTTGCTCATCCCACATACAAGAGGAAGTTGGTTGAATATGACAGAACTGAGTACACTGATATATTTGGTCGTGCAAGATGGCCCGGAGCACCACATCGTGTTTTAAAGACGCCTTTCTTCAGTGATTGGGAGTCTCTTCCTGTTCATGAAACCGAAGCTAATCAGCCAGTCATTGGTCGAACAACAATACATGACAGGGAAATAGAAATCCGTCGTCTTGGCGGTACAGTGCCAAATGTGACGACAACTGGTGACATTGAAAGCATGGTTTTGTATGCTGGCCAAAGTGTAGGCCTCATCAAGGAAATTTTACCTGCAGCAGAGGTGGTAAAGAGGCTAGTTGAAGGCGCTCAACTTCTAATCAAGCAGAAGTTCAGTTACATGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NMO

Protein Analysis

334

Amino Acids

36.07

Weight (kDa)

5.81

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NMO PF03060 6 - 75 2.7e-12 Nitronate monooxygenase
NMO PF03060 75 - 319 2.4e-44 Nitronate monooxygenase
FMN_dh PF01070 117 - 199 6.7e-10 FMN-dependent dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64250 AT5G64250
fragaria_vesca FvH4_5g07040 FvH4_5g07040
malus_domestica MD05G1043200.v1.1
prunus_persica Prupe.8G064000_v2.0.a1
pyrus_communis pycom05g03570
rosa_chinensis RchiOBHm_Chr6g0271551 RchiOBHm_Chr6g0271711
rosa_laevigata RLG00000013721 RLG00000013734
rosa_multiflora Rmu_sc0002396.1_g000003
rosa_roxburghii Rroxscaffold_7G00196850
rosa_rugosa Rorug06G0061800
rosa_samantha Rh6AG180600 Rh6BG186800 Rh6DG172300
rosa_wichuraiana Rw6G015510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 506
Acc36I ACCTGC 2 cut(s) 506, 934
AccB1I GGYRCC 1 cut(s) 46
AccB7I CCANNNNNTGG 1 cut(s) 785
AccI GTMKAC 1 cut(s) 279
AciI CCGC 3 cut(s) 224, 549, 834
AcoI YGGCCR 1 cut(s) 892
AcsI RAATTY 1 cut(s) 918
AcuI CTGAAG 1 cut(s) 715
AcyI GRCGYC 1 cut(s) 722
AdeI CACNNNGTG 1 cut(s) 710
AfaI GTAC 2 cut(s) 665, 838
AfiI CCNNNNNNNGG 3 cut(s) 350, 785, 913
AflIII ACRYGT 1 cut(s) 993
AgsI TTSAA 3 cut(s) 647, 872, 956
AjiI CACGTC 1 cut(s) 416
AjnI CCWGG 1 cut(s) 213
AluBI AGCT 7 cut(s) 79, 113, 160, 180, 304, 317, 770
AluI AGCT 7 cut(s) 79, 113, 160, 180, 304, 317, 770
Alw21I GWGCWC 2 cut(s) 568, 703
Alw26I GTCTC 1 cut(s) 750
Alw44I GTGCAC 1 cut(s) 564
AoxI GGCC 3 cut(s) 692, 892, 904
ApaLI GTGCAC 1 cut(s) 564
ApeKI GCWGC 3 cut(s) 86, 512, 929
ApoI RAATTY 1 cut(s) 918
ArsI GACNNNNNNTTYG 2 cut(s) 179, 211
AspA2I CCTAGG 1 cut(s) 580
AspLEI GCGC 1 cut(s) 962
AspS9I GGNCC 3 cut(s) 56, 71, 693
AsuC2I CCSGG 1 cut(s) 696
AsuHPI GGTGA 3 cut(s) 132, 299, 875
AvaII GGWCC 2 cut(s) 56, 71
AvrII CCTAGG 1 cut(s) 580
AxyI CCTNAGG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 568
BalI TGGCCA 1 cut(s) 894
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 127
BarI GAAGNNNNNNTAC 1 cut(s) 974
BauI CACGAG 1 cut(s) 534
Bbv12I GWGCWC 2 cut(s) 568, 703
BbvI GCAGC 3 cut(s) 73, 524, 941
BccI CCATC 1 cut(s) 684
BceAI ACGGC 1 cut(s) 74
BciT130I CCWGG 1 cut(s) 215
BclI TGATCA 1 cut(s) 435
BcnI CCSGG 1 cut(s) 696
BcoDI GTCTC 1 cut(s) 750
BfaI CTAG 4 cut(s) 110, 581, 588, 950
BfmI CTRYAG 2 cut(s) 272, 927
BfoI RGCGCY 1 cut(s) 963
BfuAI ACCTGC 2 cut(s) 506, 934
BisI GCNGC 4 cut(s) 87, 513, 549, 930
BlnI CCTAGG 1 cut(s) 580
BlsI GCNGC 4 cut(s) 88, 514, 550, 931
Bme1390I CCNGG 2 cut(s) 215, 696
Bme18I GGWCC 2 cut(s) 56, 71
BmgBI CACGTC 1 cut(s) 416
BmgT120I GGNCC 3 cut(s) 56, 71, 693
BmiI GGNNCC 3 cut(s) 48, 57, 126
BmrFI CCNGG 2 cut(s) 215, 696
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 4 cut(s) 373, 385, 436, 520
BmuI ACTGGG 1 cut(s) 292
BpuEI CTTGAG 1 cut(s) 330
BpuMI CCSGG 1 cut(s) 696
Bsa29I ATCGAT 1 cut(s) 381
BsaHI GRCGYC 1 cut(s) 722
BsaJI CCNNGG 3 cut(s) 409, 559, 580
BsaWI WCCGGW 2 cut(s) 73, 142
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 785, 913
Bse1I ACTGG 3 cut(s) 287, 779, 865
Bse21I CCTNAGG 1 cut(s) 119
Bse3DI GCAATG 1 cut(s) 507
BseBI CCWGG 1 cut(s) 215
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 3 cut(s) 409, 559, 580
BseGI GGATG 3 cut(s) 451, 535, 619
BseLI CCNNNNNNNGG 3 cut(s) 350, 785, 913
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 3 cut(s) 133, 594, 651
BseNI ACTGG 3 cut(s) 287, 779, 865
BseRI GAGGAG 1 cut(s) 377
BseSI GKGCMC 1 cut(s) 568
BseXI GCAGC 3 cut(s) 73, 524, 941
BshFI GGCC 3 cut(s) 694, 894, 906
BshNI GGYRCC 1 cut(s) 46
BshVI ATCGAT 1 cut(s) 381
BsiHKAI GWGCWC 2 cut(s) 568, 703
BsiSI CCGG 3 cut(s) 74, 143, 696
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 3 cut(s) 350, 785, 913
BsmAI GTCTC 1 cut(s) 750
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 3 cut(s) 694, 894, 906
Bsp1286I GDGCHC 3 cut(s) 127, 568, 703
Bsp143I GATC 3 cut(s) 435, 478, 490
BspACI CCGC 3 cut(s) 224, 549, 834
BspANI GGCC 3 cut(s) 694, 894, 906
BspCNI CTCAG 3 cut(s) 132, 595, 652
BspDI ATCGAT 1 cut(s) 381
BspHI TCATGA 1 cut(s) 757
BspLI GGNNCC 3 cut(s) 48, 57, 126
BspMAI CTGCAG 1 cut(s) 931
BspMI ACCTGC 2 cut(s) 506, 934
BspT107I GGYRCC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 507
BsrI ACTGG 3 cut(s) 287, 779, 865
BssECI CCNNGG 3 cut(s) 409, 559, 580
BssMI GATC 3 cut(s) 435, 478, 490
BssNAI GTATAC 1 cut(s) 280
BssNI GRCGYC 1 cut(s) 722
BssSI CACGAG 1 cut(s) 534
BssT1I CCWWGG 3 cut(s) 409, 559, 580
Bst1107I GTATAC 1 cut(s) 280
Bst2BI CACGAG 1 cut(s) 534
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 2 cut(s) 505, 841
Bst6I CTCTTC 1 cut(s) 753
BstACI GRCGYC 1 cut(s) 722
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 892
BstDEI CTNAG 3 cut(s) 119, 603, 660
BstENI CCTNNNNNAGG 2 cut(s) 348, 911
BstF5I GGATG 3 cut(s) 451, 535, 619
BstH2I RGCGCY 1 cut(s) 963
BstHHI GCGC 1 cut(s) 962
BstKTI GATC 3 cut(s) 438, 481, 493
BstMAI GTCTC 1 cut(s) 750
BstMBI GATC 3 cut(s) 435, 478, 490
BstMWI GCNNNNNNNGC 5 cut(s) 92, 374, 383, 554, 563
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 2 cut(s) 434, 997
BstSCI CCNGG 2 cut(s) 213, 694
BstSFI CTRYAG 2 cut(s) 272, 927
BstSLI GKGCMC 1 cut(s) 568
BstV1I GCAGC 3 cut(s) 73, 524, 941
BstZ17I GTATAC 1 cut(s) 280
Bsu15I ATCGAT 1 cut(s) 381
Bsu36I CCTNAGG 1 cut(s) 119
BsuRI GGCC 3 cut(s) 694, 894, 906
BsuTUI ATCGAT 1 cut(s) 381
BtrI CACGTC 1 cut(s) 416
BtsCI GGATG 3 cut(s) 451, 535, 619
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 4 cut(s) 273, 666, 739, 846
BveI ACCTGC 2 cut(s) 506, 934
Cac8I GCNNGC 1 cut(s) 892
CciI TCATGA 1 cut(s) 757
CfoI GCGC 1 cut(s) 962
Cfr13I GGNCC 3 cut(s) 56, 71, 693
ClaI ATCGAT 1 cut(s) 381
CseI GACGC 1 cut(s) 730
Csp6I GTAC 2 cut(s) 664, 837
CviAII CATG 7 cut(s) 11, 227, 431, 758, 803, 877, 994
CviQI GTAC 2 cut(s) 664, 837
DdeI CTNAG 3 cut(s) 119, 603, 660
DpnI GATC 3 cut(s) 437, 480, 492
DpnII GATC 3 cut(s) 435, 478, 490
DraI TTTAAA 1 cut(s) 717
DraIII CACNNNGTG 1 cut(s) 710
EaeI YGGCCR 1 cut(s) 892
Eam1104I CTCTTC 1 cut(s) 753
EarI CTCTTC 1 cut(s) 753
Eco130I CCWWGG 3 cut(s) 409, 559, 580
Eco147I AGGCCT 1 cut(s) 906
Eco24I GRGCYC 1 cut(s) 127
Eco47I GGWCC 2 cut(s) 56, 71
Eco57I CTGAAG 1 cut(s) 715
Eco81I CCTNAGG 1 cut(s) 119
EcoNI CCTNNNNNAGG 2 cut(s) 348, 911
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 213
EcoT14I CCWWGG 3 cut(s) 409, 559, 580
EcoT38I GRGCYC 1 cut(s) 127
ErhI CCWWGG 3 cut(s) 409, 559, 580
FaeI CATG 7 cut(s) 14, 230, 434, 761, 806, 880, 997
FaqI GGGAC 1 cut(s) 69
FatI CATG 7 cut(s) 10, 226, 430, 757, 802, 876, 993
FbaI TGATCA 1 cut(s) 435
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 4 cut(s) 87, 513, 549, 930
FokI GGATG 3 cut(s) 458, 542, 606
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 4 cut(s) 87, 513, 549, 930
FspBI CTAG 4 cut(s) 110, 581, 588, 950
GlaI GCGC 1 cut(s) 961
GluI GCNGC 4 cut(s) 87, 513, 549, 930
HaeII RGCGCY 1 cut(s) 963
HaeIII GGCC 3 cut(s) 694, 894, 906
HapII CCGG 3 cut(s) 74, 143, 696
HgaI GACGC 1 cut(s) 730
HhaI GCGC 1 cut(s) 962
Hin1I GRCGYC 1 cut(s) 722
Hin1II CATG 7 cut(s) 14, 230, 434, 761, 806, 880, 997
Hin6I GCGC 1 cut(s) 960
HinP1I GCGC 1 cut(s) 960
HindIII AAGCTT 1 cut(s) 178
HinfI GANTC 5 cut(s) 16, 137, 172, 573, 743
HpaII CCGG 3 cut(s) 74, 143, 696
HphI GGTGA 3 cut(s) 132, 299, 875
Hpy166II GTNNAC 4 cut(s) 107, 280, 566, 666
Hpy188I TCNGA 1 cut(s) 155
Hpy188III TCNNGA 4 cut(s) 69, 128, 443, 758
Hpy8I GTNNAC 4 cut(s) 107, 280, 566, 666
Hpy99I CGWCG 1 cut(s) 828
HpyAV CCTTC 1 cut(s) 950
HpyCH4III ACNGT 2 cut(s) 505, 841
HpyCH4IV ACGT 2 cut(s) 243, 415
HpyCH4V TGCA 5 cut(s) 512, 566, 599, 686, 929
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 374, 383, 554, 563
HpyF3I CTNAG 3 cut(s) 119, 603, 660
HpySE526I ACGT 2 cut(s) 243, 415
Hsp92I GRCGYC 1 cut(s) 722
Hsp92II CATG 7 cut(s) 14, 230, 434, 761, 806, 880, 997
HspAI GCGC 1 cut(s) 960
Ksp22I TGATCA 1 cut(s) 435
Kzo9I GATC 3 cut(s) 435, 478, 490
LmnI GCTCC 4 cut(s) 76, 130, 301, 698
Lsp1109I GCAGC 3 cut(s) 73, 524, 941
LweI GCATC 4 cut(s) 373, 385, 436, 520
MaeI CTAG 4 cut(s) 110, 581, 588, 950
MaeII ACGT 2 cut(s) 243, 415
MaeIII GTNAC 4 cut(s) 307, 850, 863, 989
MalI GATC 3 cut(s) 437, 480, 492
MboI GATC 3 cut(s) 435, 478, 490
MboII GAAGA 2 cut(s) 721, 740
MhlI GDGCHC 3 cut(s) 127, 568, 703
MlsI TGGCCA 1 cut(s) 894
MluCI AATT 1 cut(s) 918
MluNI TGGCCA 1 cut(s) 894
MlyI GAGTC 3 cut(s) 166, 582, 752
MmeI TCCRAC 1 cut(s) 466
Mox20I TGGCCA 1 cut(s) 894
MscI TGGCCA 1 cut(s) 894
MseI TTAA 3 cut(s) 182, 333, 716
Msp20I TGGCCA 1 cut(s) 894
MspI CCGG 3 cut(s) 74, 143, 696
MspR9I CCNGG 2 cut(s) 215, 696
MvaI CCWGG 1 cut(s) 215
MwoI GCNNNNNNNGC 5 cut(s) 92, 374, 383, 554, 563
NciI CCSGG 1 cut(s) 696
NdeII GATC 3 cut(s) 435, 478, 490
NlaIII CATG 7 cut(s) 14, 230, 434, 761, 806, 880, 997
NlaIV GGNNCC 3 cut(s) 48, 57, 126
NmuCI GTSAC 3 cut(s) 307, 850, 863
NspI RCATGY 2 cut(s) 434, 997
PagI TCATGA 1 cut(s) 757
PaqCI CACCTGC 1 cut(s) 506
PceI AGGCCT 1 cut(s) 906
PciI ACATGT 1 cut(s) 993
PfeI GAWTC 2 cut(s) 16, 137
PflMI CCANNNNNTGG 1 cut(s) 785
PkrI GCNGC 4 cut(s) 88, 514, 550, 931
PleI GAGTC 3 cut(s) 166, 581, 751
PpsI GAGTC 3 cut(s) 166, 581, 751
PpuMI RGGWCCY 1 cut(s) 56
PscI ACATGT 1 cut(s) 993
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 3 cut(s) 48, 57, 126
PspPI GGNCC 3 cut(s) 56, 71, 693
PspPPI RGGWCCY 1 cut(s) 56
PstI CTGCAG 1 cut(s) 931
RsaI GTAC 2 cut(s) 665, 838
RsaNI GTAC 2 cut(s) 664, 837
SaqAI TTAA 3 cut(s) 182, 333, 716
SatI GCNGC 4 cut(s) 87, 513, 549, 930
Sau3AI GATC 3 cut(s) 435, 478, 490
Sau96I GGNCC 3 cut(s) 56, 71, 693
SchI GAGTC 3 cut(s) 166, 582, 752
ScrFI CCNGG 2 cut(s) 215, 696
SduI GDGCHC 3 cut(s) 127, 568, 703
SfaNI GCATC 4 cut(s) 373, 385, 436, 520
SfcI CTRYAG 2 cut(s) 272, 927
SinI GGWCC 2 cut(s) 56, 71
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
Sse9I AATT 1 cut(s) 918
SseBI AGGCCT 1 cut(s) 906
SsiI CCGC 3 cut(s) 224, 549, 834
SspMI CTAG 4 cut(s) 110, 581, 588, 950
StuI AGGCCT 1 cut(s) 906
StyD4I CCNGG 2 cut(s) 213, 694
StyI CCWWGG 3 cut(s) 409, 559, 580
TaaI ACNGT 2 cut(s) 505, 841
TaiI ACGT 2 cut(s) 246, 418
TaqI TCGA 5 cut(s) 175, 381, 477, 493, 790
TasI AATT 1 cut(s) 918
TatI WGTACW 1 cut(s) 663
TauI GCSGC 1 cut(s) 551
TfiI GAWTC 2 cut(s) 16, 137
Tru1I TTAA 3 cut(s) 182, 333, 716
Tru9I TTAA 3 cut(s) 182, 333, 716
TscAI CASTG 4 cut(s) 273, 673, 739, 846
TseFI GTSAC 3 cut(s) 307, 850, 863
TseI GCWGC 3 cut(s) 86, 512, 929
Tsp45I GTSAC 3 cut(s) 307, 850, 863
TspDTI ATGAA 3 cut(s) 447, 746, 774
TspGWI ACGGA 1 cut(s) 812
TspRI CASTG 4 cut(s) 273, 673, 739, 846
Van91I CCANNNNNTGG 1 cut(s) 785
VneI GTGCAC 1 cut(s) 564
VpaK11BI GGWCC 2 cut(s) 56, 71
XagI CCTNNNNNAGG 2 cut(s) 348, 911
XapI RAATTY 1 cut(s) 918
XceI RCATGY 2 cut(s) 434, 997
XmaJI CCTAGG 1 cut(s) 580
XmiI GTMKAC 1 cut(s) 279
XspI CTAG 4 cut(s) 110, 581, 588, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.