pycom05g03570

nitronate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013392
Physical Location & Seq
Forward (+)
4132573 .. 4135603
3031 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g03570.4

Sequence Viewer

Length: 1002 bp
ATGGGTTGGCGTGGAATATTGGGTTTTGAGTACGGAATAGTTCAAGCACCGCTGGGAGCTGATATTTCAGGACCGGAGCTTGTTGCTGCTGTTGCGAATGCTGGTGGACTCGGTTTCCTCAGAGCTCCTGATTGGGAGTCACCAGATTACTTGAAAGAGCTGATAAGGAAGACTCGAAGCTTAACTAAAAAACCATTTGGGGTTGGTGTTGTTCTAGCATTTCCTCATGAGAAAAATCTACAAGCCATATTGGATGAAAAGGTTGCAGTGCTGCAGGTTTACTGGGGTGAATGTTCGGAGGAGCTTGTGTTGAAAGCTCATCAATCTGGGGTTAAGATTGTGCCTCAAGTTGGGAGCTTGGAGGAAGCGAAGCAAGCAATACATGCTGGTGTAGATGCAATTATTGTTCAAGGGCGTGAAGCAGGAGGGCATGTGATCGGTCAGGATGCTTTAATTTCATTGTTGCCGAAGGTAGTTGATCTTGTTGGAGATCGAGATATACCTCTCATTGCTGCTGGTGGCATTGTGGATGCCCGTGGATATGTTGCTGCTCTGGCCCTTGGTGCTCAGGGCATCTGCCTAGGAACTAGGTTTCTTGCAACTGAAGAAAGTCGTGCTCATCCTGCATACAAGAGAAAGGTGGTTGAATATGACGCAACTGAGTACACTGATGTATTCGGCCGTGCAAGGTGGCCCGATGCACCACATCGTGTTCTGCATACACCTTTCTTCAATGATTGTAAATATAGTCCTGCTGATCAAAATGAAGCGGGTCAACCTGTCATCGGCCGCGCAATAATACATGATAGGGAAATAGAAATTCGCCGTCTTGCCGGTACTGTTCCAAATGCGACAACAACGGGTGACATTGAAAGCATGGTGATGTATGCCGGCCAAAGTGTAGGACTCATCAAGGAAATTTTACCTGCAGGGGAGGTGGTAAAGAGGTTAGTTGAAGGGGCTCAGCTTCTGATAAAAAACAAGTTCAGTGATATACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NMO

Protein Analysis

334

Amino Acids

35.87

Weight (kDa)

5.81

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64250 AT5G64250
fragaria_vesca FvH4_5g07040 FvH4_5g07040
malus_domestica MD05G1043200.v1.1
prunus_persica Prupe.8G064000_v2.0.a1
pyrus_communis pycom05g03570
rosa_chinensis RchiOBHm_Chr6g0271551 RchiOBHm_Chr6g0271711
rosa_laevigata RLG00000013721 RLG00000013734
rosa_multiflora Rmu_sc0002396.1_g000003
rosa_roxburghii Rroxscaffold_7G00196850
rosa_rugosa Rorug06G0061800
rosa_samantha Rh6AG180600 Rh6BG186800 Rh6DG172300
rosa_wichuraiana Rw6G015510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 265, 934
AccII CGCG 1 cut(s) 792
AciI CCGC 3 cut(s) 50, 770, 790
AcoI YGGCCR 3 cut(s) 679, 787, 892
AcsI RAATTY 2 cut(s) 819, 918
AcuI CTGAAG 1 cut(s) 624
AdeI CACNNNGTG 1 cut(s) 710
AfaI GTAC 3 cut(s) 32, 665, 838
AfiI CCNNNNNNNGG 2 cut(s) 350, 785
AgsI TTSAA 8 cut(s) 44, 154, 313, 410, 647, 733, 872, 956
AluBI AGCT 9 cut(s) 59, 79, 125, 160, 180, 304, 317, 357, 967
AluI AGCT 9 cut(s) 59, 79, 125, 160, 180, 304, 317, 357, 967
Alw21I GWGCWC 3 cut(s) 127, 568, 619
AlwNI CAGNNNCTG 1 cut(s) 970
AoxI GGCC 5 cut(s) 555, 679, 692, 787, 892
ApeKI GCWGC 4 cut(s) 86, 271, 512, 548
ApoI RAATTY 2 cut(s) 819, 918
AspA2I CCTAGG 1 cut(s) 580
AspLEI GCGC 1 cut(s) 794
AspS9I GGNCC 3 cut(s) 71, 556, 693
AsuHPI GGTGA 4 cut(s) 132, 299, 875, 892
AvaII GGWCC 1 cut(s) 71
AvrII CCTAGG 1 cut(s) 580
BanII GRGCYC 2 cut(s) 127, 964
BbsI GAAGAC 1 cut(s) 176
Bbv12I GWGCWC 3 cut(s) 127, 568, 619
BbvI GCAGC 4 cut(s) 73, 258, 499, 535
BceAI ACGGC 2 cut(s) 666, 810
BclI TGATCA 1 cut(s) 757
BfaI CTAG 3 cut(s) 215, 581, 588
BfmI CTRYAG 2 cut(s) 272, 927
BfuAI ACCTGC 2 cut(s) 265, 934
BisI GCNGC 5 cut(s) 87, 272, 513, 549, 790
BlnI CCTAGG 1 cut(s) 580
BlpI GCTNAGC 1 cut(s) 963
BlsI GCNGC 5 cut(s) 88, 273, 514, 550, 791
Bme18I GGWCC 1 cut(s) 71
BmgT120I GGNCC 3 cut(s) 71, 556, 693
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 5 cut(s) 385, 436, 520, 582, 688
BmuI ACTGGG 1 cut(s) 292
BpiI GAAGAC 1 cut(s) 176
Bpu10I CCTNAGC 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 963
BpuEI CTTGAG 1 cut(s) 330
BsaJI CCNNGG 3 cut(s) 535, 559, 580
BsaWI WCCGGW 1 cut(s) 73
BsaXI ACNNNNNCTCC 4 cut(s) 293, 323, 417, 447
Bsc4I CCNNNNNNNGG 2 cut(s) 350, 785
Bse118I RCCGGY 2 cut(s) 833, 890
Bse1I ACTGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 507
BseDI CCNNGG 3 cut(s) 535, 559, 580
BseGI GGATG 4 cut(s) 259, 451, 535, 619
BseLI CCNNNNNNNGG 2 cut(s) 350, 785
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 4 cut(s) 133, 581, 651, 977
BseNI ACTGG 1 cut(s) 287
BseRI GAGGAG 1 cut(s) 314
BseX3I CGGCCG 2 cut(s) 679, 787
BseXI GCAGC 4 cut(s) 73, 258, 499, 535
BseYI CCCAGC 1 cut(s) 52
Bsh1236I CGCG 1 cut(s) 792
Bsh1285I CGRYCG 2 cut(s) 682, 790
BshFI GGCC 5 cut(s) 557, 681, 694, 789, 894
BsiEI CGRYCG 2 cut(s) 682, 790
BsiHKAI GWGCWC 3 cut(s) 127, 568, 619
BsiSI CCGG 3 cut(s) 74, 834, 891
BslI CCNNNNNNNGG 2 cut(s) 350, 785
BsmI GAATGC 1 cut(s) 103
BsnI GGCC 5 cut(s) 557, 681, 694, 789, 894
Bsp1286I GDGCHC 4 cut(s) 127, 568, 619, 964
Bsp143I GATC 4 cut(s) 435, 478, 490, 757
Bsp1720I GCTNAGC 1 cut(s) 963
BspACI CCGC 3 cut(s) 50, 770, 790
BspANI GGCC 5 cut(s) 557, 681, 694, 789, 894
BspCNI CTCAG 4 cut(s) 132, 580, 652, 976
BspFNI CGCG 1 cut(s) 792
BspHI TCATGA 1 cut(s) 226
BspMAI CTGCAG 2 cut(s) 276, 931
BspMI ACCTGC 2 cut(s) 265, 934
BsrDI GCAATG 1 cut(s) 507
BsrFI RCCGGY 2 cut(s) 833, 890
BsrI ACTGG 1 cut(s) 287
BssAI RCCGGY 2 cut(s) 833, 890
BssECI CCNNGG 3 cut(s) 535, 559, 580
BssMI GATC 4 cut(s) 435, 478, 490, 757
BssT1I CCWWGG 2 cut(s) 559, 580
Bst4CI ACNGT 2 cut(s) 841, 999
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 2 cut(s) 375, 892
BstDEI CTNAG 4 cut(s) 119, 567, 660, 963
BstDSI CCRYGG 1 cut(s) 535
BstF5I GGATG 4 cut(s) 259, 451, 535, 619
BstFNI CGCG 1 cut(s) 792
BstHHI GCGC 1 cut(s) 794
BstKTI GATC 4 cut(s) 438, 481, 493, 760
BstMBI GATC 4 cut(s) 435, 478, 490, 757
BstMCI CGRYCG 2 cut(s) 682, 790
BstMWI GCNNNNNNNGC 6 cut(s) 92, 374, 383, 554, 563, 623
BstNSI RCATGY 2 cut(s) 386, 434
BstSFI CTRYAG 2 cut(s) 272, 927
BstUI CGCG 1 cut(s) 792
BstV1I GCAGC 4 cut(s) 73, 258, 499, 535
BstV2I GAAGAC 1 cut(s) 176
BstZI CGGCCG 2 cut(s) 679, 787
BsuRI GGCC 5 cut(s) 557, 681, 694, 789, 894
BtgI CCRYGG 1 cut(s) 535
BtsCI GGATG 4 cut(s) 259, 451, 535, 619
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 3 cut(s) 273, 666, 994
BveI ACCTGC 2 cut(s) 265, 934
Cac8I GCNNGC 2 cut(s) 375, 892
CaiI CAGNNNCTG 1 cut(s) 970
CciI TCATGA 1 cut(s) 226
CfoI GCGC 1 cut(s) 794
Cfr10I RCCGGY 2 cut(s) 833, 890
Cfr13I GGNCC 3 cut(s) 71, 556, 693
CseI GACGC 1 cut(s) 662
Csp6I GTAC 3 cut(s) 31, 664, 837
CviAII CATG 5 cut(s) 227, 383, 431, 803, 877
CviQI GTAC 3 cut(s) 31, 664, 837
DdeI CTNAG 4 cut(s) 119, 567, 660, 963
DpnI GATC 4 cut(s) 437, 480, 492, 759
DpnII GATC 4 cut(s) 435, 478, 490, 757
DraIII CACNNNGTG 1 cut(s) 710
EaeI YGGCCR 3 cut(s) 679, 787, 892
EagI CGGCCG 2 cut(s) 679, 787
Ecl136II GAGCTC 1 cut(s) 125
EclXI CGGCCG 2 cut(s) 679, 787
Eco130I CCWWGG 2 cut(s) 559, 580
Eco24I GRGCYC 2 cut(s) 127, 964
Eco47I GGWCC 1 cut(s) 71
Eco52I CGGCCG 2 cut(s) 679, 787
Eco53kI GAGCTC 1 cut(s) 125
Eco57I CTGAAG 1 cut(s) 624
EcoICRI GAGCTC 1 cut(s) 125
EcoT14I CCWWGG 2 cut(s) 559, 580
EcoT38I GRGCYC 2 cut(s) 127, 964
ErhI CCWWGG 2 cut(s) 559, 580
FaeI CATG 5 cut(s) 230, 386, 434, 806, 880
FatI CATG 5 cut(s) 226, 382, 430, 802, 876
FauI CCCGC 1 cut(s) 763
FbaI TGATCA 1 cut(s) 757
Fnu4HI GCNGC 5 cut(s) 87, 272, 513, 549, 790
FokI GGATG 4 cut(s) 266, 458, 542, 606
FriOI GRGCYC 2 cut(s) 127, 964
Fsp4HI GCNGC 5 cut(s) 87, 272, 513, 549, 790
FspBI CTAG 3 cut(s) 215, 581, 588
GlaI GCGC 1 cut(s) 793
GluI GCNGC 5 cut(s) 87, 272, 513, 549, 790
GsaI CCCAGC 1 cut(s) 56
HaeIII GGCC 5 cut(s) 557, 681, 694, 789, 894
HapII CCGG 3 cut(s) 74, 834, 891
HgaI GACGC 1 cut(s) 662
HhaI GCGC 1 cut(s) 794
Hin1II CATG 5 cut(s) 230, 386, 434, 806, 880
Hin6I GCGC 1 cut(s) 792
HinP1I GCGC 1 cut(s) 792
HincII GTYRAC 1 cut(s) 776
HindII GTYRAC 1 cut(s) 776
HindIII AAGCTT 1 cut(s) 178
HinfI GANTC 4 cut(s) 108, 137, 172, 906
HpaII CCGG 3 cut(s) 74, 834, 891
HphI GGTGA 4 cut(s) 132, 299, 875, 892
Hpy166II GTNNAC 4 cut(s) 107, 280, 666, 776
Hpy188I TCNGA 3 cut(s) 122, 298, 972
Hpy188III TCNNGA 5 cut(s) 69, 128, 227, 443, 494
Hpy8I GTNNAC 4 cut(s) 107, 280, 666, 776
HpyAV CCTTC 2 cut(s) 463, 950
HpyCH4III ACNGT 2 cut(s) 841, 999
HpyCH4V TGCA 9 cut(s) 266, 274, 398, 599, 626, 686, 701, 718, 929
HpyF10VI GCNNNNNNNGC 6 cut(s) 92, 374, 383, 554, 563, 623
HpyF3I CTNAG 4 cut(s) 119, 567, 660, 963
Hsp92II CATG 5 cut(s) 230, 386, 434, 806, 880
HspAI GCGC 1 cut(s) 792
KroI GCCGGC 1 cut(s) 890
KroNI GCCGGC 1 cut(s) 892
Ksp22I TGATCA 1 cut(s) 757
Kzo9I GATC 4 cut(s) 435, 478, 490, 757
LmnI GCTCC 5 cut(s) 56, 76, 130, 301, 354
Lsp1109I GCAGC 4 cut(s) 73, 258, 499, 535
LweI GCATC 5 cut(s) 385, 436, 520, 582, 688
MaeI CTAG 3 cut(s) 215, 581, 588
MaeIII GTNAC 2 cut(s) 138, 863
MalI GATC 4 cut(s) 437, 480, 492, 759
MboI GATC 4 cut(s) 435, 478, 490, 757
MboII GAAGA 3 cut(s) 181, 617, 721
MhlI GDGCHC 4 cut(s) 127, 568, 619, 964
MluCI AATT 4 cut(s) 399, 453, 819, 918
MlyI GAGTC 4 cut(s) 102, 146, 166, 900
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 9 cut(s) 128, 234, 292, 354, 355, 419, 513, 928, 939
MroNI GCCGGC 1 cut(s) 890
MseI TTAA 3 cut(s) 182, 333, 452
MslI CAYNNNNRTG 2 cut(s) 387, 881
MspA1I CMGCKG 1 cut(s) 52
MspI CCGG 3 cut(s) 74, 834, 891
Mva1269I GAATGC 1 cut(s) 103
MvnI CGCG 1 cut(s) 792
MwoI GCNNNNNNNGC 6 cut(s) 92, 374, 383, 554, 563, 623
NaeI GCCGGC 1 cut(s) 892
NdeII GATC 4 cut(s) 435, 478, 490, 757
NgoMIV GCCGGC 1 cut(s) 890
NlaIII CATG 5 cut(s) 230, 386, 434, 806, 880
NmuCI GTSAC 2 cut(s) 138, 863
NspI RCATGY 2 cut(s) 386, 434
PagI TCATGA 1 cut(s) 226
PctI GAATGC 1 cut(s) 103
PdiI GCCGGC 1 cut(s) 892
PkrI GCNGC 5 cut(s) 88, 273, 514, 550, 791
PleI GAGTC 4 cut(s) 102, 145, 166, 900
PpsI GAGTC 4 cut(s) 102, 145, 166, 900
Psp124BI GAGCTC 1 cut(s) 127
PspFI CCCAGC 1 cut(s) 52
PspPI GGNCC 3 cut(s) 71, 556, 693
PstI CTGCAG 2 cut(s) 276, 931
PstNI CAGNNNCTG 1 cut(s) 970
RsaI GTAC 3 cut(s) 32, 665, 838
RsaNI GTAC 3 cut(s) 31, 664, 837
RseI CAYNNNNRTG 2 cut(s) 387, 881
SacI GAGCTC 1 cut(s) 127
SaqAI TTAA 3 cut(s) 182, 333, 452
SatI GCNGC 5 cut(s) 87, 272, 513, 549, 790
Sau3AI GATC 4 cut(s) 435, 478, 490, 757
Sau96I GGNCC 3 cut(s) 71, 556, 693
SbfI CCTGCAGG 1 cut(s) 931
SchI GAGTC 4 cut(s) 102, 146, 166, 900
SdaI CCTGCAGG 1 cut(s) 931
SduI GDGCHC 4 cut(s) 127, 568, 619, 964
SfaNI GCATC 5 cut(s) 385, 436, 520, 582, 688
SfcI CTRYAG 2 cut(s) 272, 927
SinI GGWCC 1 cut(s) 71
SmiMI CAYNNNNRTG 2 cut(s) 387, 881
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
Sse8387I CCTGCAGG 1 cut(s) 931
Sse9I AATT 4 cut(s) 399, 453, 819, 918
SsiI CCGC 3 cut(s) 50, 770, 790
SspI AATATT 1 cut(s) 18
SspMI CTAG 3 cut(s) 215, 581, 588
SstI GAGCTC 1 cut(s) 127
StyI CCWWGG 2 cut(s) 559, 580
TaaI ACNGT 2 cut(s) 841, 999
TaqI TCGA 2 cut(s) 175, 493
TaqII GACCGA 1 cut(s) 428
TasI AATT 4 cut(s) 399, 453, 819, 918
TatI WGTACW 1 cut(s) 663
TauI GCSGC 1 cut(s) 792
Tru1I TTAA 3 cut(s) 182, 333, 452
Tru9I TTAA 3 cut(s) 182, 333, 452
TscAI CASTG 3 cut(s) 273, 673, 994
TseFI GTSAC 2 cut(s) 138, 863
TseI GCWGC 4 cut(s) 86, 271, 512, 548
Tsp45I GTSAC 2 cut(s) 138, 863
TspDTI ATGAA 3 cut(s) 270, 447, 780
TspGWI ACGGA 1 cut(s) 48
TspRI CASTG 3 cut(s) 273, 673, 994
VpaK11BI GGWCC 1 cut(s) 71
XapI RAATTY 2 cut(s) 819, 918
XceI RCATGY 2 cut(s) 386, 434
XmaJI CCTAGG 1 cut(s) 580
XspI CTAG 3 cut(s) 215, 581, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.