MD05G1043200.v1.1

nitronate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013392
Physical Location & Seq
Forward (+)
7354174 .. 7357392
3219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1043200.v1.1.491

Sequence Viewer

Length: 999 bp
ATGGGTTGGCGTGGAATATTGGGTTTTGAGTACGGAATAGTTCAAGCACCGCTGGGAGCTGATATTTCAGGACCGGAGCTTGTTGCTGCTGTTGCGAATGCTGGTGGACTCGGTTTCCTCAGAGCTCCTGATTGGTCACCAGATTACTTGAAAGAGCTGATAAGGAAGACTCGAAGCTTAACTAAAAAACCATTTGGGGTTGGTGTTGTTCTAGCATTTCCTCATGAGAAAAATATACAAGCCATATTGGATGAAAAGGTTGCAGTGCTGCAGGTTTACTGGGGTGAATGTTCGGAGGAGCTTGTGTTGAAAGCTCATCAATCTGGGGTTAAGATTGTGCCTCAAGTTGGGAGCTTGGAGGAAGCGAAGAAAGCAATACATGCTGGTGTAGATGCAATTATTGTTCAAGGGCATGAAGCAGGAGGGCATGTGATCGGTCAGGATGCTTTAATTTCATTGTTGCCGAAGGTAGTTGATCTTGTTGGAGATCGAGATATACCTCTCATTGCTGCTGGTGGTATTGTGGATGCCCGTGGATATGTTGCTGCTCTGGCCCTTGGTGCTCAGGGCATCTGCCTAGGAACTAGGTTTCTTGCAACTGAAGAAAGTCATGCTCATCCTGCATACAAGAGAAAGGTGGTTGAATATGACGCAACTGAGTACACTGATGTATTCGGCCGTGCAAGGTGGCCTGATGCACCACATCGTGTTCTGCATACACCTTTCTTCAATGATTGTAAATATATTCCTGCTGATCAAAATGAAGCGGGTCAACCTGTTATTGGCTGCGCAATAATACATGATAGGGAAATAGAAATTCACCGTCTTGCCGGTGTTGTTCCAAATGCGACAACAACGGGTGACATTGAAAGCATGGTGATGTATGCCGGCCAAAGTGTAGGACTCATCAAGGAAATTTTACCTGCAGGGGAGGTGGTAAAGAGGTTAGTTGAAGGGGCTCAGCTTCTGATAAAAAACAAGTTCAGTGATATACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NMO

Protein Analysis

333

Amino Acids

35.65

Weight (kDa)

5.78

Isoelectric Point (pI)

38.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NMO PF03060 6 - 72 9.5e-15 Nitronate monooxygenase
NMO PF03060 74 - 318 3.7e-45 Nitronate monooxygenase
IMPDH PF00478 75 - 207 3e-06 IMP dehydrogenase / GMP reductase domain
FMN_dh PF01070 111 - 198 1.5e-10 FMN-dependent dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64250 AT5G64250
fragaria_vesca FvH4_5g07040 FvH4_5g07040
malus_domestica MD05G1043200.v1.1
prunus_persica Prupe.8G064000_v2.0.a1
pyrus_communis pycom05g03570
rosa_chinensis RchiOBHm_Chr6g0271551 RchiOBHm_Chr6g0271711
rosa_laevigata RLG00000013721 RLG00000013734
rosa_multiflora Rmu_sc0002396.1_g000003
rosa_roxburghii Rroxscaffold_7G00196850
rosa_rugosa Rorug06G0061800
rosa_samantha Rh6AG180600 Rh6BG186800 Rh6DG172300
rosa_wichuraiana Rw6G015510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 790
Acc36I ACCTGC 2 cut(s) 262, 931
AciI CCGC 2 cut(s) 50, 767
AcoI YGGCCR 2 cut(s) 676, 889
AcsI RAATTY 2 cut(s) 816, 915
AcuI CTGAAG 1 cut(s) 621
AdeI CACNNNGTG 1 cut(s) 707
AfaI GTAC 2 cut(s) 32, 662
AfiI CCNNNNNNNGG 2 cut(s) 347, 782
AgsI TTSAA 8 cut(s) 44, 151, 310, 407, 644, 730, 869, 953
AluBI AGCT 9 cut(s) 59, 79, 125, 157, 177, 301, 314, 354, 964
AluI AGCT 9 cut(s) 59, 79, 125, 157, 177, 301, 314, 354, 964
Alw21I GWGCWC 2 cut(s) 127, 565
AlwNI CAGNNNCTG 1 cut(s) 967
AoxI GGCC 4 cut(s) 552, 676, 689, 889
ApeKI GCWGC 5 cut(s) 86, 268, 509, 545, 786
ApoI RAATTY 2 cut(s) 816, 915
AspA2I CCTAGG 1 cut(s) 577
AspLEI GCGC 1 cut(s) 791
AspS9I GGNCC 2 cut(s) 71, 553
AsuHPI GGTGA 5 cut(s) 129, 296, 812, 872, 889
AvaII GGWCC 1 cut(s) 71
AvrII CCTAGG 1 cut(s) 577
BanII GRGCYC 2 cut(s) 127, 961
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 2 cut(s) 127, 565
BbvI GCAGC 5 cut(s) 73, 255, 496, 532, 773
BceAI ACGGC 1 cut(s) 663
BclI TGATCA 1 cut(s) 754
BfaI CTAG 3 cut(s) 212, 578, 585
BfmI CTRYAG 2 cut(s) 269, 924
BfuAI ACCTGC 2 cut(s) 262, 931
BisI GCNGC 5 cut(s) 87, 269, 510, 546, 787
BlnI CCTAGG 1 cut(s) 577
BlpI GCTNAGC 1 cut(s) 960
BlsI GCNGC 5 cut(s) 88, 270, 511, 547, 788
Bme18I GGWCC 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 71, 553
BmrI ACTGGG 1 cut(s) 289
BmsI GCATC 5 cut(s) 382, 433, 517, 579, 685
BmuI ACTGGG 1 cut(s) 289
BpiI GAAGAC 1 cut(s) 173
Bpu10I CCTNAGC 1 cut(s) 564
Bpu1102I GCTNAGC 1 cut(s) 960
BpuEI CTTGAG 1 cut(s) 327
BsaJI CCNNGG 3 cut(s) 532, 556, 577
BsaWI WCCGGW 1 cut(s) 73
BsaXI ACNNNNNCTCC 4 cut(s) 290, 320, 414, 444
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 782
Bse118I RCCGGY 2 cut(s) 830, 887
Bse1I ACTGG 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 504
BseDI CCNNGG 3 cut(s) 532, 556, 577
BseGI GGATG 4 cut(s) 256, 448, 532, 616
BseLI CCNNNNNNNGG 2 cut(s) 347, 782
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 4 cut(s) 133, 578, 648, 974
BseNI ACTGG 1 cut(s) 284
BseRI GAGGAG 1 cut(s) 311
BseX3I CGGCCG 1 cut(s) 676
BseXI GCAGC 5 cut(s) 73, 255, 496, 532, 773
BseYI CCCAGC 1 cut(s) 52
Bsh1285I CGRYCG 1 cut(s) 679
BshFI GGCC 4 cut(s) 554, 678, 691, 891
BsiEI CGRYCG 1 cut(s) 679
BsiHKAI GWGCWC 2 cut(s) 127, 565
BsiSI CCGG 3 cut(s) 74, 831, 888
BslI CCNNNNNNNGG 2 cut(s) 347, 782
BsmI GAATGC 1 cut(s) 103
BsnI GGCC 4 cut(s) 554, 678, 691, 891
Bsp1286I GDGCHC 3 cut(s) 127, 565, 961
Bsp143I GATC 4 cut(s) 432, 475, 487, 754
Bsp1720I GCTNAGC 1 cut(s) 960
BspACI CCGC 2 cut(s) 50, 767
BspANI GGCC 4 cut(s) 554, 678, 691, 891
BspCNI CTCAG 4 cut(s) 132, 577, 649, 973
BspHI TCATGA 1 cut(s) 223
BspMAI CTGCAG 2 cut(s) 273, 928
BspMI ACCTGC 2 cut(s) 262, 931
BsrDI GCAATG 1 cut(s) 504
BsrFI RCCGGY 2 cut(s) 830, 887
BsrI ACTGG 1 cut(s) 284
BssAI RCCGGY 2 cut(s) 830, 887
BssECI CCNNGG 3 cut(s) 532, 556, 577
BssMI GATC 4 cut(s) 432, 475, 487, 754
BssT1I CCWWGG 2 cut(s) 556, 577
Bst4CI ACNGT 2 cut(s) 824, 996
BstAPI GCANNNNNTGC 1 cut(s) 380
BstC8I GCNNGC 1 cut(s) 889
BstDEI CTNAG 4 cut(s) 119, 564, 657, 960
BstDSI CCRYGG 1 cut(s) 532
BstEII GGTNACC 1 cut(s) 135
BstF5I GGATG 4 cut(s) 256, 448, 532, 616
BstHHI GCGC 1 cut(s) 791
BstKTI GATC 4 cut(s) 435, 478, 490, 757
BstMBI GATC 4 cut(s) 432, 475, 487, 754
BstMCI CGRYCG 1 cut(s) 679
BstMWI GCNNNNNNNGC 6 cut(s) 92, 371, 380, 551, 560, 620
BstNSI RCATGY 2 cut(s) 383, 431
BstPI GGTNACC 1 cut(s) 135
BstSFI CTRYAG 2 cut(s) 269, 924
BstV1I GCAGC 5 cut(s) 73, 255, 496, 532, 773
BstV2I GAAGAC 1 cut(s) 173
BstZI CGGCCG 1 cut(s) 676
BsuRI GGCC 4 cut(s) 554, 678, 691, 891
BtgI CCRYGG 1 cut(s) 532
BtsCI GGATG 4 cut(s) 256, 448, 532, 616
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 3 cut(s) 270, 663, 991
BveI ACCTGC 2 cut(s) 262, 931
Cac8I GCNNGC 1 cut(s) 889
CaiI CAGNNNCTG 1 cut(s) 967
CciI TCATGA 1 cut(s) 223
CfoI GCGC 1 cut(s) 791
Cfr10I RCCGGY 2 cut(s) 830, 887
Cfr13I GGNCC 2 cut(s) 71, 553
CseI GACGC 1 cut(s) 659
Csp6I GTAC 2 cut(s) 31, 661
CviAII CATG 7 cut(s) 224, 380, 413, 428, 611, 800, 874
CviQI GTAC 2 cut(s) 31, 661
DdeI CTNAG 4 cut(s) 119, 564, 657, 960
DpnI GATC 4 cut(s) 434, 477, 489, 756
DpnII GATC 4 cut(s) 432, 475, 487, 754
DraIII CACNNNGTG 1 cut(s) 707
EaeI YGGCCR 2 cut(s) 676, 889
EagI CGGCCG 1 cut(s) 676
Ecl136II GAGCTC 1 cut(s) 125
EclXI CGGCCG 1 cut(s) 676
Eco130I CCWWGG 2 cut(s) 556, 577
Eco24I GRGCYC 2 cut(s) 127, 961
Eco47I GGWCC 1 cut(s) 71
Eco52I CGGCCG 1 cut(s) 676
Eco53kI GAGCTC 1 cut(s) 125
Eco57I CTGAAG 1 cut(s) 621
Eco91I GGTNACC 1 cut(s) 135
EcoICRI GAGCTC 1 cut(s) 125
EcoO65I GGTNACC 1 cut(s) 135
EcoT14I CCWWGG 2 cut(s) 556, 577
EcoT38I GRGCYC 2 cut(s) 127, 961
ErhI CCWWGG 2 cut(s) 556, 577
FaeI CATG 7 cut(s) 227, 383, 416, 431, 614, 803, 877
FatI CATG 7 cut(s) 223, 379, 412, 427, 610, 799, 873
FauI CCCGC 1 cut(s) 760
FbaI TGATCA 1 cut(s) 754
Fnu4HI GCNGC 5 cut(s) 87, 269, 510, 546, 787
FokI GGATG 4 cut(s) 263, 455, 539, 603
FriOI GRGCYC 2 cut(s) 127, 961
Fsp4HI GCNGC 5 cut(s) 87, 269, 510, 546, 787
FspBI CTAG 3 cut(s) 212, 578, 585
FspI TGCGCA 1 cut(s) 790
GlaI GCGC 1 cut(s) 790
GluI GCNGC 5 cut(s) 87, 269, 510, 546, 787
GsaI CCCAGC 1 cut(s) 56
HaeIII GGCC 4 cut(s) 554, 678, 691, 891
HapII CCGG 3 cut(s) 74, 831, 888
HgaI GACGC 1 cut(s) 659
HhaI GCGC 1 cut(s) 791
Hin1II CATG 7 cut(s) 227, 383, 416, 431, 614, 803, 877
Hin6I GCGC 1 cut(s) 789
HinP1I GCGC 1 cut(s) 789
HincII GTYRAC 1 cut(s) 773
HindII GTYRAC 1 cut(s) 773
HindIII AAGCTT 1 cut(s) 175
HinfI GANTC 3 cut(s) 108, 169, 903
HpaII CCGG 3 cut(s) 74, 831, 888
HphI GGTGA 5 cut(s) 129, 296, 812, 872, 889
Hpy166II GTNNAC 4 cut(s) 107, 277, 663, 773
Hpy188I TCNGA 3 cut(s) 122, 295, 969
Hpy188III TCNNGA 5 cut(s) 69, 128, 224, 440, 491
Hpy8I GTNNAC 4 cut(s) 107, 277, 663, 773
HpyAV CCTTC 2 cut(s) 460, 947
HpyCH4III ACNGT 2 cut(s) 824, 996
HpyCH4V TGCA 9 cut(s) 263, 271, 395, 596, 623, 683, 698, 715, 926
HpyF10VI GCNNNNNNNGC 6 cut(s) 92, 371, 380, 551, 560, 620
HpyF3I CTNAG 4 cut(s) 119, 564, 657, 960
Hsp92II CATG 7 cut(s) 227, 383, 416, 431, 614, 803, 877
HspAI GCGC 1 cut(s) 789
KroI GCCGGC 1 cut(s) 887
KroNI GCCGGC 1 cut(s) 889
Ksp22I TGATCA 1 cut(s) 754
Kzo9I GATC 4 cut(s) 432, 475, 487, 754
LmnI GCTCC 5 cut(s) 56, 76, 130, 298, 351
Lsp1109I GCAGC 5 cut(s) 73, 255, 496, 532, 773
LweI GCATC 5 cut(s) 382, 433, 517, 579, 685
MaeI CTAG 3 cut(s) 212, 578, 585
MaeIII GTNAC 2 cut(s) 135, 860
MalI GATC 4 cut(s) 434, 477, 489, 756
MboI GATC 4 cut(s) 432, 475, 487, 754
MboII GAAGA 4 cut(s) 178, 379, 614, 718
MhlI GDGCHC 3 cut(s) 127, 565, 961
MluCI AATT 4 cut(s) 396, 450, 816, 915
MlyI GAGTC 3 cut(s) 102, 163, 897
MmeI TCCRAC 1 cut(s) 463
MnlI CCTC 9 cut(s) 128, 231, 289, 351, 352, 416, 510, 925, 936
MroNI GCCGGC 1 cut(s) 887
MseI TTAA 3 cut(s) 179, 330, 449
MslI CAYNNNNRTG 2 cut(s) 384, 878
MspA1I CMGCKG 1 cut(s) 52
MspI CCGG 3 cut(s) 74, 831, 888
Mva1269I GAATGC 1 cut(s) 103
MwoI GCNNNNNNNGC 6 cut(s) 92, 371, 380, 551, 560, 620
NaeI GCCGGC 1 cut(s) 889
NdeII GATC 4 cut(s) 432, 475, 487, 754
NgoMIV GCCGGC 1 cut(s) 887
NlaIII CATG 7 cut(s) 227, 383, 416, 431, 614, 803, 877
NmuCI GTSAC 2 cut(s) 135, 860
NsbI TGCGCA 1 cut(s) 790
NspI RCATGY 2 cut(s) 383, 431
PagI TCATGA 1 cut(s) 223
PctI GAATGC 1 cut(s) 103
PdiI GCCGGC 1 cut(s) 889
PkrI GCNGC 5 cut(s) 88, 270, 511, 547, 788
PleI GAGTC 3 cut(s) 102, 163, 897
PpsI GAGTC 3 cut(s) 102, 163, 897
Psp124BI GAGCTC 1 cut(s) 127
PspEI GGTNACC 1 cut(s) 135
PspFI CCCAGC 1 cut(s) 52
PspPI GGNCC 2 cut(s) 71, 553
PstI CTGCAG 2 cut(s) 273, 928
PstNI CAGNNNCTG 1 cut(s) 967
RsaI GTAC 2 cut(s) 32, 662
RsaNI GTAC 2 cut(s) 31, 661
RseI CAYNNNNRTG 2 cut(s) 384, 878
SacI GAGCTC 1 cut(s) 127
SaqAI TTAA 3 cut(s) 179, 330, 449
SatI GCNGC 5 cut(s) 87, 269, 510, 546, 787
Sau3AI GATC 4 cut(s) 432, 475, 487, 754
Sau96I GGNCC 2 cut(s) 71, 553
SbfI CCTGCAGG 1 cut(s) 928
SchI GAGTC 3 cut(s) 102, 163, 897
SdaI CCTGCAGG 1 cut(s) 928
SduI GDGCHC 3 cut(s) 127, 565, 961
SfaNI GCATC 5 cut(s) 382, 433, 517, 579, 685
SfcI CTRYAG 2 cut(s) 269, 924
SinI GGWCC 1 cut(s) 71
SmiMI CAYNNNNRTG 2 cut(s) 384, 878
SmlI CTYRAG 1 cut(s) 342
SmoI CTYRAG 1 cut(s) 342
Sse8387I CCTGCAGG 1 cut(s) 928
Sse9I AATT 4 cut(s) 396, 450, 816, 915
SsiI CCGC 2 cut(s) 50, 767
SspI AATATT 1 cut(s) 18
SspMI CTAG 3 cut(s) 212, 578, 585
SstI GAGCTC 1 cut(s) 127
StyI CCWWGG 2 cut(s) 556, 577
TaaI ACNGT 2 cut(s) 824, 996
TaqI TCGA 2 cut(s) 172, 490
TaqII GACCGA 1 cut(s) 425
TasI AATT 4 cut(s) 396, 450, 816, 915
TatI WGTACW 1 cut(s) 660
Tru1I TTAA 3 cut(s) 179, 330, 449
Tru9I TTAA 3 cut(s) 179, 330, 449
TscAI CASTG 3 cut(s) 270, 670, 991
TseFI GTSAC 2 cut(s) 135, 860
TseI GCWGC 5 cut(s) 86, 268, 509, 545, 786
Tsp45I GTSAC 2 cut(s) 135, 860
TspDTI ATGAA 4 cut(s) 267, 429, 444, 777
TspGWI ACGGA 1 cut(s) 48
TspRI CASTG 3 cut(s) 270, 670, 991
VpaK11BI GGWCC 1 cut(s) 71
XapI RAATTY 2 cut(s) 816, 915
XceI RCATGY 2 cut(s) 383, 431
XmaJI CCTAGG 1 cut(s) 577
XspI CTAG 3 cut(s) 212, 578, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.