Prupe.8G064000_v2.0.a1

nitronate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0013392
Physical Location & Seq
Reverse (-)
9418757 .. 9423070
4314 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G064000.2

Sequence Viewer

Length: 1014 bp
ATGGGTTGGGGTGGGATATTGGGTTTTGAGTATGGAATTGTTCAAGCACCATTGGGACCTGATATTGCTGGACCCGAGCTTGTTGCTGCTGTTGCTAATGCTGGGGGACTTGGTTTCCTCAGAGCCCCTGATTGGGAGTCACCACATTACCTGCGGGAGCTCATAAGGAAGACTCGAAGCTTAACTGACAAACCGTTTGGGGTTGGTGTTGTTCTGGCGTTTCCGCATGAGAAAAATATACAAGTCATTTTGGATGAAAAGGTAGCAGTGCTGCAGGTTTACTGGGGTGAATGTTCAGAGGAGCTTGTATTGAAAGCTCATCAGTCTGGGGTTAAGGTTGTTCCTCAAGTTGGTAGCTTGGACGAAGCGAATAAAGCAATATATGCTGGTGTAGATGCAATTATTGTTCAAGGGCGTGAAGCAGGAGGGCATGTGATCAGTCAGGATGCTTTGATTTCATTGTTGCCCAAGGTAGCTGATCTTGTTGGAGATCGGGATATACCTGTTATCGCGGCCGGTGGTATAGTGGATGCCCGTGGATATGTTGCTGCTCTGGCCCTTGGTGCTCAAGGGGTCTGCCTAGGCACTAGGTTTCTTGCAACTCAGGAAAGCCGTGCTCATCCCACATACAAGAGGAAGCTGGTTGAATTTGACAGAACTGAGTACACTGATGTATTCGGGCGTGCAAGGTGGCCTGGAGCGCCGCATCGCGTTCTGTATACACCATTCTTCAATGATTGGAAATCTCTTCCTCCTCATCAGAATGAAGCAGATCAGCCCGTCATCGGTCATTCGACGATACATGATAGGGAAATAGAAATTCGTCGTCTTGCCGGTACTGTTCCAAATGTGACTGCAACTGGTGACATTGAAAGCATGGTGATGTATGCTGGCCAAAGTGTAGGCCTCATCAAGGAAATTTTACCTGCAGGGGAGGTGGTAAAGAGGCTAGTTGAAGAGGCTCAACTTCTGATAAAGCACAAGTTCAGTGATATATTGTTGACTCATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NMO

Protein Analysis

338

Amino Acids

36.54

Weight (kDa)

5.89

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64250 AT5G64250
fragaria_vesca FvH4_5g07040 FvH4_5g07040
malus_domestica MD05G1043200.v1.1
prunus_persica Prupe.8G064000_v2.0.a1
pyrus_communis pycom05g03570
rosa_chinensis RchiOBHm_Chr6g0271551 RchiOBHm_Chr6g0271711
rosa_laevigata RLG00000013721 RLG00000013734
rosa_multiflora Rmu_sc0002396.1_g000003
rosa_roxburghii Rroxscaffold_7G00196850
rosa_rugosa Rorug06G0061800
rosa_samantha Rh6AG180600 Rh6BG186800 Rh6DG172300
rosa_wichuraiana Rw6G015510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 159, 265, 934
AccI GTMKAC 1 cut(s) 719
AccII CGCG 2 cut(s) 512, 711
AciI CCGC 4 cut(s) 154, 224, 512, 704
AcoI YGGCCR 2 cut(s) 513, 892
AcsI RAATTY 3 cut(s) 647, 819, 918
AfaI GTAC 2 cut(s) 665, 838
AfiI CCNNNNNNNGG 5 cut(s) 132, 133, 350, 785, 913
AgsI TTSAA 7 cut(s) 44, 313, 410, 647, 733, 872, 956
AjnI CCWGG 1 cut(s) 694
AluBI AGCT 9 cut(s) 79, 160, 180, 304, 317, 357, 476, 640, 1011
AluI AGCT 9 cut(s) 79, 160, 180, 304, 317, 357, 476, 640, 1011
Alw21I GWGCWC 3 cut(s) 162, 568, 619
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 5 cut(s) 513, 555, 692, 892, 904
ApeKI GCWGC 3 cut(s) 86, 271, 548
ApoI RAATTY 3 cut(s) 647, 819, 918
ArsI GACNNNNNNTTYG 2 cut(s) 179, 211
AspA2I CCTAGG 1 cut(s) 580
AspLEI GCGC 1 cut(s) 703
AspS9I GGNCC 3 cut(s) 56, 71, 556
AsuHPI GGTGA 4 cut(s) 132, 299, 875, 892
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 2 cut(s) 56, 71
AvrII CCTAGG 1 cut(s) 580
BalI TGGCCA 1 cut(s) 894
BanII GRGCYC 2 cut(s) 127, 162
BbsI GAAGAC 1 cut(s) 176
Bbv12I GWGCWC 3 cut(s) 162, 568, 619
BbvI GCAGC 3 cut(s) 73, 258, 535
BceAI ACGGC 1 cut(s) 597
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 1 cut(s) 696
BclI TGATCA 1 cut(s) 435
BfaI CTAG 3 cut(s) 581, 588, 950
BfmI CTRYAG 2 cut(s) 272, 927
BfoI RGCGCY 1 cut(s) 704
BfuAI ACCTGC 3 cut(s) 159, 265, 934
BisI GCNGC 5 cut(s) 87, 272, 513, 549, 704
BlnI CCTAGG 1 cut(s) 580
BlsI GCNGC 5 cut(s) 88, 273, 514, 550, 705
Bme1390I CCNGG 1 cut(s) 696
Bme18I GGWCC 2 cut(s) 56, 71
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 3 cut(s) 56, 71, 556
BmiI GGNNCC 2 cut(s) 57, 73
BmrFI CCNGG 1 cut(s) 696
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 4 cut(s) 385, 436, 520, 715
BmuI ACTGGG 1 cut(s) 292
BpiI GAAGAC 1 cut(s) 176
BpmI CTGGAG 1 cut(s) 717
BpuEI CTTGAG 2 cut(s) 330, 552
BsaJI CCNNGG 4 cut(s) 468, 535, 559, 580
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 5 cut(s) 132, 133, 350, 785, 913
Bse118I RCCGGY 2 cut(s) 515, 833
Bse1I ACTGG 2 cut(s) 287, 865
BseBI CCWGG 1 cut(s) 696
BseDI CCNNGG 4 cut(s) 468, 535, 559, 580
BseGI GGATG 4 cut(s) 259, 451, 535, 619
BseLI CCNNNNNNNGG 5 cut(s) 132, 133, 350, 785, 913
BseMII CTCAG 3 cut(s) 133, 617, 651
BseNI ACTGG 2 cut(s) 287, 865
BseRI GAGGAG 2 cut(s) 314, 744
BseX3I CGGCCG 1 cut(s) 513
BseXI GCAGC 3 cut(s) 73, 258, 535
BseYI CCCAGC 1 cut(s) 101
Bsh1236I CGCG 2 cut(s) 512, 711
Bsh1285I CGRYCG 1 cut(s) 516
BshFI GGCC 5 cut(s) 515, 557, 694, 894, 906
BsiEI CGRYCG 1 cut(s) 516
BsiHKAI GWGCWC 3 cut(s) 162, 568, 619
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 2 cut(s) 516, 834
BslFI GGGAC 2 cut(s) 69, 120
BslI CCNNNNNNNGG 5 cut(s) 132, 133, 350, 785, 913
BsmFI GGGAC 2 cut(s) 69, 120
BsnI GGCC 5 cut(s) 515, 557, 694, 894, 906
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 4 cut(s) 127, 162, 568, 619
Bsp143I GATC 4 cut(s) 435, 478, 490, 772
BspACI CCGC 4 cut(s) 154, 224, 512, 704
BspANI GGCC 5 cut(s) 515, 557, 694, 894, 906
BspCNI CTCAG 3 cut(s) 132, 616, 652
BspFNI CGCG 2 cut(s) 512, 711
BspLI GGNNCC 2 cut(s) 57, 73
BspMAI CTGCAG 2 cut(s) 276, 931
BspMI ACCTGC 3 cut(s) 159, 265, 934
BsrFI RCCGGY 2 cut(s) 515, 833
BsrI ACTGG 2 cut(s) 287, 865
BssAI RCCGGY 2 cut(s) 515, 833
BssECI CCNNGG 4 cut(s) 468, 535, 559, 580
BssMI GATC 4 cut(s) 435, 478, 490, 772
BssNAI GTATAC 1 cut(s) 720
BssT1I CCWWGG 3 cut(s) 468, 559, 580
Bst1107I GTATAC 1 cut(s) 720
Bst2UI CCWGG 1 cut(s) 696
Bst4CI ACNGT 2 cut(s) 195, 841
Bst6I CTCTTC 2 cut(s) 753, 951
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 2 cut(s) 684, 892
BstDEI CTNAG 3 cut(s) 119, 603, 660
BstDSI CCRYGG 1 cut(s) 535
BstENI CCTNNNNNAGG 1 cut(s) 911
BstF5I GGATG 4 cut(s) 259, 451, 535, 619
BstFNI CGCG 2 cut(s) 512, 711
BstH2I RGCGCY 1 cut(s) 704
BstHHI GCGC 1 cut(s) 703
BstKTI GATC 4 cut(s) 438, 481, 493, 775
BstMBI GATC 4 cut(s) 435, 478, 490, 772
BstMCI CGRYCG 1 cut(s) 516
BstMWI GCNNNNNNNGC 6 cut(s) 92, 374, 383, 554, 563, 700
BstNI CCWGG 1 cut(s) 696
BstNSI RCATGY 1 cut(s) 434
BstSCI CCNGG 1 cut(s) 694
BstSFI CTRYAG 2 cut(s) 272, 927
BstUI CGCG 2 cut(s) 512, 711
BstV1I GCAGC 3 cut(s) 73, 258, 535
BstV2I GAAGAC 1 cut(s) 176
BstZ17I GTATAC 1 cut(s) 720
BstZI CGGCCG 1 cut(s) 513
BsuRI GGCC 5 cut(s) 515, 557, 694, 894, 906
BtgI CCRYGG 1 cut(s) 535
BtgZI GCGATG 1 cut(s) 692
BtsCI GGATG 4 cut(s) 259, 451, 535, 619
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 3 cut(s) 273, 666, 994
BveI ACCTGC 3 cut(s) 159, 265, 934
Cac8I GCNNGC 2 cut(s) 684, 892
CfoI GCGC 1 cut(s) 703
Cfr10I RCCGGY 2 cut(s) 515, 833
Cfr13I GGNCC 3 cut(s) 56, 71, 556
Csp6I GTAC 2 cut(s) 664, 837
CviAII CATG 4 cut(s) 227, 431, 803, 877
CviQI GTAC 2 cut(s) 664, 837
DdeI CTNAG 3 cut(s) 119, 603, 660
DpnI GATC 4 cut(s) 437, 480, 492, 774
DpnII GATC 4 cut(s) 435, 478, 490, 772
EaeI YGGCCR 2 cut(s) 513, 892
EagI CGGCCG 1 cut(s) 513
Eam1104I CTCTTC 2 cut(s) 753, 951
EarI CTCTTC 2 cut(s) 753, 951
Ecl136II GAGCTC 1 cut(s) 160
EclXI CGGCCG 1 cut(s) 513
Eco130I CCWWGG 3 cut(s) 468, 559, 580
Eco147I AGGCCT 1 cut(s) 906
Eco24I GRGCYC 2 cut(s) 127, 162
Eco47I GGWCC 2 cut(s) 56, 71
Eco52I CGGCCG 1 cut(s) 513
Eco53kI GAGCTC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 74
EcoICRI GAGCTC 1 cut(s) 160
EcoNI CCTNNNNNAGG 1 cut(s) 911
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 694
EcoT14I CCWWGG 3 cut(s) 468, 559, 580
EcoT38I GRGCYC 2 cut(s) 127, 162
ErhI CCWWGG 3 cut(s) 468, 559, 580
FaeI CATG 4 cut(s) 230, 434, 806, 880
FaqI GGGAC 2 cut(s) 69, 120
FatI CATG 4 cut(s) 226, 430, 802, 876
FauI CCCGC 1 cut(s) 147
FbaI TGATCA 1 cut(s) 435
FblI GTMKAC 1 cut(s) 719
Fnu4HI GCNGC 5 cut(s) 87, 272, 513, 549, 704
FokI GGATG 4 cut(s) 266, 458, 542, 606
FriOI GRGCYC 2 cut(s) 127, 162
Fsp4HI GCNGC 5 cut(s) 87, 272, 513, 549, 704
FspBI CTAG 3 cut(s) 581, 588, 950
GlaI GCGC 1 cut(s) 702
GluI GCNGC 5 cut(s) 87, 272, 513, 549, 704
GsaI CCCAGC 1 cut(s) 105
GsuI CTGGAG 1 cut(s) 717
HaeII RGCGCY 1 cut(s) 704
HaeIII GGCC 5 cut(s) 515, 557, 694, 894, 906
HapII CCGG 2 cut(s) 516, 834
HhaI GCGC 1 cut(s) 703
Hin1II CATG 4 cut(s) 230, 434, 806, 880
Hin6I GCGC 1 cut(s) 701
HinP1I GCGC 1 cut(s) 701
HincII GTYRAC 1 cut(s) 1002
HindII GTYRAC 1 cut(s) 1002
HindIII AAGCTT 1 cut(s) 178
HinfI GANTC 3 cut(s) 137, 172, 1003
HpaII CCGG 2 cut(s) 516, 834
HphI GGTGA 4 cut(s) 132, 299, 875, 892
Hpy166II GTNNAC 4 cut(s) 280, 666, 720, 1002
Hpy188I TCNGA 4 cut(s) 122, 298, 762, 972
Hpy188III TCNNGA 3 cut(s) 443, 494, 605
Hpy8I GTNNAC 4 cut(s) 280, 666, 720, 1002
Hpy99I CGWCG 2 cut(s) 799, 828
HpyCH4III ACNGT 2 cut(s) 195, 841
HpyCH4V TGCA 6 cut(s) 274, 398, 599, 686, 857, 929
HpyF10VI GCNNNNNNNGC 6 cut(s) 92, 374, 383, 554, 563, 700
HpyF3I CTNAG 3 cut(s) 119, 603, 660
Hsp92II CATG 4 cut(s) 230, 434, 806, 880
HspAI GCGC 1 cut(s) 701
Ksp22I TGATCA 1 cut(s) 435
Kzo9I GATC 4 cut(s) 435, 478, 490, 772
LmnI GCTCC 3 cut(s) 157, 301, 698
Lsp1109I GCAGC 3 cut(s) 73, 258, 535
LweI GCATC 4 cut(s) 385, 436, 520, 715
MaeI CTAG 3 cut(s) 581, 588, 950
MaeIII GTNAC 3 cut(s) 138, 850, 863
MalI GATC 4 cut(s) 437, 480, 492, 774
MboI GATC 4 cut(s) 435, 478, 490, 772
MboII GAAGA 4 cut(s) 181, 721, 740, 968
MhlI GDGCHC 4 cut(s) 127, 162, 568, 619
MlsI TGGCCA 1 cut(s) 894
MluCI AATT 5 cut(s) 36, 399, 647, 819, 918
MluNI TGGCCA 1 cut(s) 894
MlyI GAGTC 3 cut(s) 146, 166, 997
MmeI TCCRAC 1 cut(s) 466
Mox20I TGGCCA 1 cut(s) 894
MscI TGGCCA 1 cut(s) 894
MseI TTAA 2 cut(s) 182, 333
MslI CAYNNNNRTG 2 cut(s) 762, 881
Msp20I TGGCCA 1 cut(s) 894
MspI CCGG 2 cut(s) 516, 834
MspR9I CCNGG 1 cut(s) 696
MvaI CCWGG 1 cut(s) 696
MvnI CGCG 2 cut(s) 512, 711
MwoI GCNNNNNNNGC 6 cut(s) 92, 374, 383, 554, 563, 700
NdeII GATC 4 cut(s) 435, 478, 490, 772
NlaIII CATG 4 cut(s) 230, 434, 806, 880
NlaIV GGNNCC 2 cut(s) 57, 73
NmuCI GTSAC 3 cut(s) 138, 850, 863
NspI RCATGY 1 cut(s) 434
PceI AGGCCT 1 cut(s) 906
PkrI GCNGC 5 cut(s) 88, 273, 514, 550, 705
PleI GAGTC 3 cut(s) 145, 166, 997
PpsI GAGTC 3 cut(s) 145, 166, 997
PpuMI RGGWCCY 1 cut(s) 56
Psp124BI GAGCTC 1 cut(s) 162
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 694
PspFI CCCAGC 1 cut(s) 101
PspGI CCWGG 1 cut(s) 694
PspN4I GGNNCC 2 cut(s) 57, 73
PspPI GGNCC 3 cut(s) 56, 71, 556
PspPPI RGGWCCY 1 cut(s) 56
PstI CTGCAG 2 cut(s) 276, 931
RsaI GTAC 2 cut(s) 665, 838
RsaNI GTAC 2 cut(s) 664, 837
RseI CAYNNNNRTG 2 cut(s) 762, 881
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 2 cut(s) 182, 333
SatI GCNGC 5 cut(s) 87, 272, 513, 549, 704
Sau3AI GATC 4 cut(s) 435, 478, 490, 772
Sau96I GGNCC 3 cut(s) 56, 71, 556
SbfI CCTGCAGG 1 cut(s) 931
SchI GAGTC 3 cut(s) 146, 166, 997
ScrFI CCNGG 1 cut(s) 696
SdaI CCTGCAGG 1 cut(s) 931
SduI GDGCHC 4 cut(s) 127, 162, 568, 619
SfaNI GCATC 4 cut(s) 385, 436, 520, 715
SfcI CTRYAG 2 cut(s) 272, 927
SinI GGWCC 2 cut(s) 56, 71
SmiMI CAYNNNNRTG 2 cut(s) 762, 881
SmlI CTYRAG 2 cut(s) 345, 567
SmoI CTYRAG 2 cut(s) 345, 567
Sse8387I CCTGCAGG 1 cut(s) 931
Sse9I AATT 5 cut(s) 36, 399, 647, 819, 918
SseBI AGGCCT 1 cut(s) 906
SsiI CCGC 4 cut(s) 154, 224, 512, 704
SspMI CTAG 3 cut(s) 581, 588, 950
SstI GAGCTC 1 cut(s) 162
StuI AGGCCT 1 cut(s) 906
StyD4I CCNGG 1 cut(s) 694
StyI CCWWGG 3 cut(s) 468, 559, 580
TaaI ACNGT 2 cut(s) 195, 841
TaqI TCGA 2 cut(s) 175, 794
TaqII GACCGA 1 cut(s) 776
TasI AATT 5 cut(s) 36, 399, 647, 819, 918
TatI WGTACW 1 cut(s) 663
TauI GCSGC 2 cut(s) 515, 706
Tru1I TTAA 2 cut(s) 182, 333
Tru9I TTAA 2 cut(s) 182, 333
TscAI CASTG 3 cut(s) 273, 673, 994
TseFI GTSAC 3 cut(s) 138, 850, 863
TseI GCWGC 3 cut(s) 86, 271, 548
Tsp45I GTSAC 3 cut(s) 138, 850, 863
TspDTI ATGAA 3 cut(s) 270, 447, 780
TspRI CASTG 3 cut(s) 273, 673, 994
VpaK11BI GGWCC 2 cut(s) 56, 71
XagI CCTNNNNNAGG 1 cut(s) 911
XapI RAATTY 3 cut(s) 647, 819, 918
XceI RCATGY 1 cut(s) 434
XmaJI CCTAGG 1 cut(s) 580
XmiI GTMKAC 1 cut(s) 719
XspI CTAG 3 cut(s) 581, 588, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.