FvH4_5g07040

nitronate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013392
Physical Location & Seq
Reverse (-)
4155062 .. 4160059
4998 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g07040.t4

Sequence Viewer

Length: 996 bp
ATGGGTTGGCGTGGGATTCTGGGTTTTGAGTATGGAATAGTTCAGGCACCTTTGGGACCTGATATTTCTGGACCTGAGCTTGTTGCTGCTGTTGCTAATGCTGGCGGACTAGCTTTCCTCCGGGCTCCTGATTGGGAATCACCAGACTATCTCCGGGAGCTTATAAGTAAGACTCGAAGCTTAACGGACAAACCATTTGGGGTTGCTGTTGTCCTGGCATTTCCACATGAGAAAAATATAAACGTCATATTGGAGGAAAAGGTAGCAGTTCTGCAGGTATACTGGGGTGATTGTTCTGCGGAGCTTGTGATGAAAGCTCATCGTTCTGGGGTTAAGATTGTTCCTCAGGTGGGGAGTTTGGAGGAGGCCGAAAAAGCAATCTCTGCTGGTATAGATGCAATTATTGTCCAAGGATTAGAAGCAGGAGGACACGTGATCAGTCAGGATGCTTTGATTTCATTGTTGCCAAAGGTAGTCAATCTTGTCGGAGATCAAAATATAACCATCATTGCAGCAGGTGGTATTGTGGATGCTAGCGGGTATGTTGCCGCCCTTGCCCTTGGTGCACAAGGAATCTGCCTAGGCACTAGGTTTCTTGCAACCGAGGAAAGTTATGCTCATCCCATATACAAGAGGAAGTTGGTTGAGTATGACAGAACTGCGTACACTGATATATTTGGCCGTGCAAGATGGCCTGGAGCACCACATCGTGTTTTGGAGACACCTTTCTTCAATGAGTGGAAGTCTCTTCCTGATCATGAAACAGAAGCCGATCAGCCAGTCATTGGTCGAACAAGAATACATGACAGGGAAATAGAAATCCGTCGTCTTGCCGGGACAGTTCCAAATGTAACCACAACTGGTGACATTGAAAGCATGGTTTTCTATGCTGGCCAAAGTGTAGGCCTTATCAAGGAAATATTACCTGCAGCAGAAGTGGTAAAGAGGCTAGTTGAAGGCGCTCAACTTCTAATCAAGCAGAAGTTCAGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NMO

Protein Analysis

332

Amino Acids

35.86

Weight (kDa)

5.56

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NMO PF03060 2 - 216 1e-37 Nitronate monooxygenase
FMN_dh PF01070 14 - 96 7.8e-09 FMN-dependent dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64250 AT5G64250
fragaria_vesca FvH4_5g07040 FvH4_5g07040
malus_domestica MD05G1043200.v1.1
prunus_persica Prupe.8G064000_v2.0.a1
pyrus_communis pycom05g03570
rosa_chinensis RchiOBHm_Chr6g0271551 RchiOBHm_Chr6g0271711
rosa_laevigata RLG00000013721 RLG00000013734
rosa_multiflora Rmu_sc0002396.1_g000003
rosa_roxburghii Rroxscaffold_7G00196850
rosa_rugosa Rorug06G0061800
rosa_samantha Rh6AG180600 Rh6BG186800 Rh6DG172300
rosa_wichuraiana Rw6G015510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 164
AarI CACCTGC 1 cut(s) 506
Acc36I ACCTGC 3 cut(s) 265, 506, 934
AccB1I GGYRCC 1 cut(s) 46
AccB7I CCANNNNNTGG 1 cut(s) 785
AccI GTMKAC 1 cut(s) 279
AciI CCGC 4 cut(s) 105, 299, 537, 549
AcoI YGGCCR 2 cut(s) 679, 892
AcvI CACGTG 1 cut(s) 433
AdeI CACNNNGTG 1 cut(s) 710
AfaI GTAC 1 cut(s) 665
AfiI CCNNNNNNNGG 3 cut(s) 350, 785, 913
AflIII ACRYGT 1 cut(s) 430
AgsI TTSAA 3 cut(s) 733, 872, 956
AjnI CCWGG 2 cut(s) 213, 694
AluBI AGCT 6 cut(s) 79, 113, 160, 180, 304, 317
AluI AGCT 6 cut(s) 79, 113, 160, 180, 304, 317
Alw21I GWGCWC 2 cut(s) 568, 703
Alw26I GTCTC 2 cut(s) 713, 750
Alw44I GTGCAC 1 cut(s) 564
AoxI GGCC 5 cut(s) 366, 679, 692, 892, 904
ApaLI GTGCAC 1 cut(s) 564
ApeKI GCWGC 3 cut(s) 86, 512, 929
ArsI GACNNNNNNTTYG 2 cut(s) 179, 211
AspA2I CCTAGG 1 cut(s) 580
AspLEI GCGC 1 cut(s) 962
AspS9I GGNCC 2 cut(s) 56, 71
AsuC2I CCSGG 3 cut(s) 122, 155, 835
AsuHPI GGTGA 3 cut(s) 132, 299, 875
AsuNHI GCTAGC 1 cut(s) 533
AvaII GGWCC 2 cut(s) 56, 71
AvrII CCTAGG 1 cut(s) 580
AxyI CCTNAGG 1 cut(s) 345
BaeGI GKGCMC 1 cut(s) 568
BalI TGGCCA 1 cut(s) 894
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 127
BbrPI CACGTG 1 cut(s) 433
Bbv12I GWGCWC 2 cut(s) 568, 703
BbvI GCAGC 3 cut(s) 73, 524, 941
BccI CCATC 2 cut(s) 512, 684
BceAI ACGGC 1 cut(s) 666
BciT130I CCWGG 2 cut(s) 215, 696
BclI TGATCA 2 cut(s) 435, 754
BcnI CCSGG 3 cut(s) 122, 155, 835
BcoDI GTCTC 2 cut(s) 713, 750
BfaI CTAG 6 cut(s) 110, 534, 581, 588, 950, 994
BfmI CTRYAG 2 cut(s) 272, 927
BfoI RGCGCY 1 cut(s) 963
BfuAI ACCTGC 3 cut(s) 265, 506, 934
BisI GCNGC 4 cut(s) 87, 513, 549, 930
BlnI CCTAGG 1 cut(s) 580
BlsI GCNGC 4 cut(s) 88, 514, 550, 931
Bme1390I CCNGG 5 cut(s) 122, 155, 215, 696, 835
Bme18I GGWCC 2 cut(s) 56, 71
BmgT120I GGNCC 2 cut(s) 56, 71
BmiI GGNNCC 3 cut(s) 48, 57, 126
BmrFI CCNGG 5 cut(s) 122, 155, 215, 696, 835
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 3 cut(s) 385, 436, 520
BmtI GCTAGC 1 cut(s) 537
BmuI ACTGGG 1 cut(s) 292
BpmI CTGGAG 1 cut(s) 717
Bpu10I CCTNAGC 1 cut(s) 75
BpuMI CCSGG 3 cut(s) 122, 155, 835
BsaAI YACGTR 1 cut(s) 433
BsaJI CCNNGG 4 cut(s) 409, 559, 580, 603
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 785, 913
Bse1I ACTGG 3 cut(s) 287, 779, 865
Bse21I CCTNAGG 1 cut(s) 345
Bse3DI GCAATG 1 cut(s) 507
BseBI CCWGG 2 cut(s) 215, 696
BseDI CCNNGG 4 cut(s) 409, 559, 580, 603
BseGI GGATG 3 cut(s) 451, 535, 619
BseLI CCNNNNNNNGG 3 cut(s) 350, 785, 913
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 2 cut(s) 66, 359
BseNI ACTGG 3 cut(s) 287, 779, 865
BseRI GAGGAG 1 cut(s) 377
BseSI GKGCMC 1 cut(s) 568
BseXI GCAGC 3 cut(s) 73, 524, 941
BshFI GGCC 5 cut(s) 368, 681, 694, 894, 906
BshNI GGYRCC 1 cut(s) 46
BsiHKAI GWGCWC 2 cut(s) 568, 703
BsiSI CCGG 3 cut(s) 121, 154, 834
BslFI GGGAC 2 cut(s) 69, 850
BslI CCNNNNNNNGG 3 cut(s) 350, 785, 913
BsmAI GTCTC 2 cut(s) 713, 750
BsmFI GGGAC 2 cut(s) 69, 850
BsnI GGCC 5 cut(s) 368, 681, 694, 894, 906
Bsp1286I GDGCHC 3 cut(s) 127, 568, 703
Bsp143I GATC 4 cut(s) 435, 490, 754, 772
BspACI CCGC 4 cut(s) 105, 299, 537, 549
BspANI GGCC 5 cut(s) 368, 681, 694, 894, 906
BspCNI CTCAG 2 cut(s) 67, 358
BspHI TCATGA 1 cut(s) 757
BspLI GGNNCC 3 cut(s) 48, 57, 126
BspMAI CTGCAG 2 cut(s) 276, 931
BspMI ACCTGC 3 cut(s) 265, 506, 934
BspOI GCTAGC 1 cut(s) 537
BspT107I GGYRCC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 507
BsrI ACTGG 3 cut(s) 287, 779, 865
BssECI CCNNGG 4 cut(s) 409, 559, 580, 603
BssMI GATC 4 cut(s) 435, 490, 754, 772
BssNAI GTATAC 1 cut(s) 280
BssT1I CCWWGG 3 cut(s) 409, 559, 580
Bst1107I GTATAC 1 cut(s) 280
Bst2UI CCWGG 2 cut(s) 215, 696
Bst4CI ACNGT 1 cut(s) 841
Bst6I CTCTTC 1 cut(s) 753
BstAPI GCANNNNNTGC 1 cut(s) 383
BstBAI YACGTR 1 cut(s) 433
BstC8I GCNNGC 3 cut(s) 103, 535, 892
BstDEI CTNAG 2 cut(s) 75, 345
BstENI CCTNNNNNAGG 1 cut(s) 911
BstF5I GGATG 3 cut(s) 451, 535, 619
BstH2I RGCGCY 1 cut(s) 963
BstHHI GCGC 1 cut(s) 962
BstKTI GATC 4 cut(s) 438, 493, 757, 775
BstMAI GTCTC 2 cut(s) 713, 750
BstMBI GATC 4 cut(s) 435, 490, 754, 772
BstMWI GCNNNNNNNGC 5 cut(s) 92, 374, 383, 554, 563
BstNI CCWGG 2 cut(s) 215, 696
BstSCI CCNGG 5 cut(s) 120, 153, 213, 694, 833
BstSFI CTRYAG 2 cut(s) 272, 927
BstSLI GKGCMC 1 cut(s) 568
BstV1I GCAGC 3 cut(s) 73, 524, 941
BstZ17I GTATAC 1 cut(s) 280
Bsu36I CCTNAGG 1 cut(s) 345
BsuRI GGCC 5 cut(s) 368, 681, 694, 894, 906
BtsCI GGATG 3 cut(s) 451, 535, 619
BtsIMutI CAGTG 1 cut(s) 666
BveI ACCTGC 3 cut(s) 265, 506, 934
Cac8I GCNNGC 3 cut(s) 103, 535, 892
CciI TCATGA 1 cut(s) 757
CfoI GCGC 1 cut(s) 962
Cfr13I GGNCC 2 cut(s) 56, 71
Csp6I GTAC 1 cut(s) 664
CviAII CATG 4 cut(s) 227, 758, 803, 877
CviQI GTAC 1 cut(s) 664
DdeI CTNAG 2 cut(s) 75, 345
DpnI GATC 4 cut(s) 437, 492, 756, 774
DpnII GATC 4 cut(s) 435, 490, 754, 772
DraIII CACNNNGTG 1 cut(s) 710
EaeI YGGCCR 2 cut(s) 679, 892
Eam1104I CTCTTC 1 cut(s) 753
EarI CTCTTC 1 cut(s) 753
EciI GGCGGA 1 cut(s) 120
Eco130I CCWWGG 3 cut(s) 409, 559, 580
Eco147I AGGCCT 1 cut(s) 906
Eco24I GRGCYC 1 cut(s) 127
Eco47I GGWCC 2 cut(s) 56, 71
Eco72I CACGTG 1 cut(s) 433
Eco81I CCTNAGG 1 cut(s) 345
EcoNI CCTNNNNNAGG 1 cut(s) 911
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 2 cut(s) 213, 694
EcoT14I CCWWGG 3 cut(s) 409, 559, 580
EcoT38I GRGCYC 1 cut(s) 127
ErhI CCWWGG 3 cut(s) 409, 559, 580
FaeI CATG 4 cut(s) 230, 761, 806, 880
FaqI GGGAC 2 cut(s) 69, 850
FatI CATG 4 cut(s) 226, 757, 802, 876
FauI CCCGC 1 cut(s) 530
FbaI TGATCA 2 cut(s) 435, 754
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 4 cut(s) 87, 513, 549, 930
FokI GGATG 3 cut(s) 458, 542, 606
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 4 cut(s) 87, 513, 549, 930
FspBI CTAG 6 cut(s) 110, 534, 581, 588, 950, 994
GlaI GCGC 1 cut(s) 961
GluI GCNGC 4 cut(s) 87, 513, 549, 930
GsuI CTGGAG 1 cut(s) 717
HaeII RGCGCY 1 cut(s) 963
HaeIII GGCC 5 cut(s) 368, 681, 694, 894, 906
HapII CCGG 3 cut(s) 121, 154, 834
HhaI GCGC 1 cut(s) 962
Hin1II CATG 4 cut(s) 230, 761, 806, 880
Hin6I GCGC 1 cut(s) 960
HinP1I GCGC 1 cut(s) 960
HindIII AAGCTT 1 cut(s) 178
HinfI GANTC 4 cut(s) 16, 137, 172, 573
HpaII CCGG 3 cut(s) 121, 154, 834
HphI GGTGA 3 cut(s) 132, 299, 875
Hpy166II GTNNAC 3 cut(s) 280, 566, 666
Hpy188I TCNGA 1 cut(s) 488
Hpy188III TCNNGA 5 cut(s) 69, 128, 443, 752, 758
Hpy8I GTNNAC 3 cut(s) 280, 566, 666
Hpy99I CGWCG 1 cut(s) 828
HpyAV CCTTC 1 cut(s) 950
HpyCH4III ACNGT 1 cut(s) 841
HpyCH4IV ACGT 2 cut(s) 243, 432
HpyCH4V TGCA 7 cut(s) 274, 398, 512, 566, 599, 686, 929
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 374, 383, 554, 563
HpyF3I CTNAG 2 cut(s) 75, 345
HpySE526I ACGT 2 cut(s) 243, 432
Hsp92II CATG 4 cut(s) 230, 761, 806, 880
HspAI GCGC 1 cut(s) 960
Ksp22I TGATCA 2 cut(s) 435, 754
Kzo9I GATC 4 cut(s) 435, 490, 754, 772
LmnI GCTCC 4 cut(s) 130, 157, 301, 698
Lsp1109I GCAGC 3 cut(s) 73, 524, 941
LweI GCATC 3 cut(s) 385, 436, 520
MaeI CTAG 6 cut(s) 110, 534, 581, 588, 950, 994
MaeII ACGT 2 cut(s) 243, 432
MaeIII GTNAC 3 cut(s) 850, 863, 989
MalI GATC 4 cut(s) 437, 492, 756, 774
MboI GATC 4 cut(s) 435, 490, 754, 772
MboII GAAGA 2 cut(s) 721, 740
MhlI GDGCHC 3 cut(s) 127, 568, 703
MlsI TGGCCA 1 cut(s) 894
MluCI AATT 1 cut(s) 399
MluNI TGGCCA 1 cut(s) 894
MlyI GAGTC 1 cut(s) 166
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 9 cut(s) 128, 247, 354, 355, 358, 419, 598, 627, 939
Mox20I TGGCCA 1 cut(s) 894
MscI TGGCCA 1 cut(s) 894
MseI TTAA 2 cut(s) 182, 333
Msp20I TGGCCA 1 cut(s) 894
MspI CCGG 3 cut(s) 121, 154, 834
MspR9I CCNGG 5 cut(s) 122, 155, 215, 696, 835
MvaI CCWGG 2 cut(s) 215, 696
MwoI GCNNNNNNNGC 5 cut(s) 92, 374, 383, 554, 563
NciI CCSGG 3 cut(s) 122, 155, 835
NdeII GATC 4 cut(s) 435, 490, 754, 772
NheI GCTAGC 1 cut(s) 533
NlaIII CATG 4 cut(s) 230, 761, 806, 880
NlaIV GGNNCC 3 cut(s) 48, 57, 126
NmuCI GTSAC 2 cut(s) 863, 989
PagI TCATGA 1 cut(s) 757
PaqCI CACCTGC 1 cut(s) 506
PceI AGGCCT 1 cut(s) 906
PfeI GAWTC 3 cut(s) 16, 137, 573
PflMI CCANNNNNTGG 1 cut(s) 785
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 4 cut(s) 88, 514, 550, 931
PleI GAGTC 1 cut(s) 166
PmaCI CACGTG 1 cut(s) 433
PmlI CACGTG 1 cut(s) 433
PpsI GAGTC 1 cut(s) 166
Ppu21I YACGTR 1 cut(s) 433
PpuMI RGGWCCY 1 cut(s) 56
PsiI TTATAA 1 cut(s) 164
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 2 cut(s) 213, 694
PspCI CACGTG 1 cut(s) 433
PspGI CCWGG 2 cut(s) 213, 694
PspN4I GGNNCC 3 cut(s) 48, 57, 126
PspPI GGNCC 2 cut(s) 56, 71
PspPPI RGGWCCY 1 cut(s) 56
PstI CTGCAG 2 cut(s) 276, 931
RsaI GTAC 1 cut(s) 665
RsaNI GTAC 1 cut(s) 664
SaqAI TTAA 2 cut(s) 182, 333
SatI GCNGC 4 cut(s) 87, 513, 549, 930
Sau3AI GATC 4 cut(s) 435, 490, 754, 772
Sau96I GGNCC 2 cut(s) 56, 71
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 5 cut(s) 122, 155, 215, 696, 835
SduI GDGCHC 3 cut(s) 127, 568, 703
SfaNI GCATC 3 cut(s) 385, 436, 520
SfcI CTRYAG 2 cut(s) 272, 927
SinI GGWCC 2 cut(s) 56, 71
Sse9I AATT 1 cut(s) 399
SseBI AGGCCT 1 cut(s) 906
SsiI CCGC 4 cut(s) 105, 299, 537, 549
SspI AATATT 1 cut(s) 921
SspMI CTAG 6 cut(s) 110, 534, 581, 588, 950, 994
StuI AGGCCT 1 cut(s) 906
StyD4I CCNGG 5 cut(s) 120, 153, 213, 694, 833
StyI CCWWGG 3 cut(s) 409, 559, 580
TaaI ACNGT 1 cut(s) 841
TaiI ACGT 2 cut(s) 246, 435
TaqI TCGA 2 cut(s) 175, 790
TasI AATT 1 cut(s) 399
TauI GCSGC 1 cut(s) 551
TfiI GAWTC 3 cut(s) 16, 137, 573
Tru1I TTAA 2 cut(s) 182, 333
Tru9I TTAA 2 cut(s) 182, 333
TscAI CASTG 1 cut(s) 673
TseFI GTSAC 2 cut(s) 863, 989
TseI GCWGC 3 cut(s) 86, 512, 929
Tsp45I GTSAC 2 cut(s) 863, 989
TspDTI ATGAA 3 cut(s) 326, 447, 774
TspGWI ACGGA 2 cut(s) 200, 812
TspRI CASTG 1 cut(s) 673
Van91I CCANNNNNTGG 1 cut(s) 785
VneI GTGCAC 1 cut(s) 564
VpaK11BI GGWCC 2 cut(s) 56, 71
XagI CCTNNNNNAGG 1 cut(s) 911
XmaJI CCTAGG 1 cut(s) 580
XmiI GTMKAC 1 cut(s) 279
XspI CTAG 6 cut(s) 110, 534, 581, 588, 950, 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.