AT5G66010

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016692
Physical Location & Seq
Forward (+)
26399432 .. 26401050
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66010.2

Sequence Viewer

Length: 768 bp
ATGTACGGATCTAGGGGGGCCATGTTTGGGAGCGGGGGGTACGAGGTGGGATCAAAGAGACAAAGAATGATGCAATCAAATCCCTACTTGGCAGTTGGCACTGGACCTACCAGCTTTCCACCTTTTGGCTATGCCGGCGGATTCCCAGTCGTTCGCCTCAGGGGTCTTCCCTTCAACTGCGCTGACATTGACATCTTCGAGTTCTTTGCGGGCCTCAACATTGTCGATGTCCTGCTCGTCAGCAAAAACGGTAAATTCTCTGGGGAGGCCTTTGTGGTGTTTGCCGGCCCTATGCAAGTCGAGATTGCCTTGCAAAGGGACAGACACAATATGGGGAGGAGATACGTGGAAGTTTTCCGGTGCTCTAAGCAGGACTACTACAATGCGGTGGCTGCTGAGGAGGGAGCGTATGAGTATGAGGTACGTGCTAGCCCACCTCCCACCGGGCCATCCAGGGCAAAGAGGTTTAGTGAGAAAGAGAAGCTTGAGTACACAGAGGTTTTGAAGATGCGAGGCCTCCCTTACTCGGTGAACAAACCTCAAATCATAGAGTTTTTCAGCGGGTACAAGGTTATCCAAGGACGGGTACAGGTTGTGTGTCGGCCTGATGGGAAGGCCACGGGAGAGGCATTTGTGGAGTTTGAGACGGGGGAGGAGGCGAGGAGGGCAATGGCTAAGGACAAAATGTCGATTGGGTCAAGGTATGTGGAGTTGTTTCCAACTACACGTGAAGAGGCTCGAAGGGCTGAGGCCAGATCTAGGCAATGA

Protein Analysis

255

Amino Acids

28.59

Weight (kDa)

9.26

Isoelectric Point (pI)

48.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 73 - 139 7.1e-10 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016692)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66010 AT5G66010
fragaria_vesca FvH4_1g10440
malus_domestica MD02G1117600.v1.1 MD15G1232300.v1.1
prunus_persica Prupe.7G181800_v2.0.a1
pyrus_communis pycom15g20670
rosa_chinensis RchiOBHm_Chr2g0097301
rosa_laevigata RLG00000016715
rosa_roxburghii Rroxscaffold_2G00144800
rosa_samantha Rh2AG112300 Rh2BG115000 Rh2CG117100
rosa_wichuraiana Rw2G008780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 125
AccBSI CCGCTC 1 cut(s) 33
AciI CCGC 5 cut(s) 33, 138, 209, 386, 561
AclWI GGATC 2 cut(s) 16, 58
AcsI RAATTY 1 cut(s) 254
AcvI CACGTG 1 cut(s) 728
AfaI GTAC 6 cut(s) 5, 41, 423, 491, 566, 588
AfiI CCNNNNNNNGG 7 cut(s) 27, 125, 315, 443, 526, 583, 759
AflIII ACRYGT 1 cut(s) 725
AgsI TTSAA 2 cut(s) 175, 505
AhdI GACNNNNNGTC 1 cut(s) 685
AjnI CCWGG 1 cut(s) 452
AluBI AGCT 2 cut(s) 114, 484
AluI AGCT 2 cut(s) 114, 484
Alw21I GWGCWC 1 cut(s) 365
Alw26I GTCTC 2 cut(s) 52, 638
AlwI GGATC 2 cut(s) 16, 58
AoxI GGCC 9 cut(s) 18, 211, 267, 286, 446, 514, 602, 615, 750
ApeKI GCWGC 1 cut(s) 392
ApoI RAATTY 1 cut(s) 254
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 5 cut(s) 18, 104, 211, 287, 446
AsuC2I CCSGG 1 cut(s) 445
AsuHPI GGTGA 1 cut(s) 541
AsuNHI GCTAGC 1 cut(s) 428
AvaII GGWCC 1 cut(s) 104
AxyI CCTNAGG 1 cut(s) 158
BarI GAAGNNNNNNTAC 2 cut(s) 473, 505
BbrPI CACGTG 1 cut(s) 728
BbsI GAAGAC 1 cut(s) 158
Bbv12I GWGCWC 1 cut(s) 365
BbvCI CCTCAGC 2 cut(s) 396, 747
BbvI GCAGC 1 cut(s) 379
BccI CCATC 2 cut(s) 457, 602
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciT130I CCWGG 1 cut(s) 454
BcnI CCSGG 1 cut(s) 445
BcoDI GTCTC 2 cut(s) 52, 638
BfaI CTAG 3 cut(s) 12, 429, 759
BglII AGATCT 1 cut(s) 755
BisI GCNGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 394
Bme1390I CCNGG 2 cut(s) 445, 454
Bme18I GGWCC 1 cut(s) 104
BmeRI GACNNNNNGTC 1 cut(s) 685
BmgT120I GGNCC 5 cut(s) 18, 104, 211, 287, 446
BmiI GGNNCC 1 cut(s) 19
BmrFI CCNGG 2 cut(s) 445, 454
BmrI ACTGGG 1 cut(s) 140
BmsI GCATC 2 cut(s) 60, 498
BmtI GCTAGC 1 cut(s) 432
BmuI ACTGGG 1 cut(s) 140
BpiI GAAGAC 1 cut(s) 158
Bpu10I CCTNAGC 3 cut(s) 396, 675, 747
BpuEI CTTGAG 1 cut(s) 506
BpuMI CCSGG 1 cut(s) 445
BsaAI YACGTR 3 cut(s) 346, 425, 728
BsaJI CCNNGG 3 cut(s) 453, 577, 618
BsaWI WCCGGW 1 cut(s) 357
BsaXI ACNNNNNCTCC 2 cut(s) 392, 422
Bsc4I CCNNNNNNNGG 7 cut(s) 27, 125, 315, 443, 526, 583, 759
Bse118I RCCGGY 2 cut(s) 134, 284
Bse1I ACTGG 2 cut(s) 106, 146
Bse21I CCTNAGG 1 cut(s) 158
Bse3DI GCAATG 1 cut(s) 675
BseBI CCWGG 1 cut(s) 454
BseDI CCNNGG 3 cut(s) 453, 577, 618
BseGI GGATG 1 cut(s) 449
BseLI CCNNNNNNNGG 7 cut(s) 27, 125, 315, 443, 526, 583, 759
BseMI GCAATG 1 cut(s) 675
BseMII CTCAG 3 cut(s) 172, 387, 738
BseNI ACTGG 2 cut(s) 106, 146
BseRI GAGGAG 4 cut(s) 352, 413, 668, 676
BseXI GCAGC 1 cut(s) 379
BshFI GGCC 9 cut(s) 20, 213, 269, 288, 448, 516, 604, 617, 752
BsiHKAI GWGCWC 1 cut(s) 365
BsiSI CCGG 4 cut(s) 135, 285, 358, 444
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 7 cut(s) 27, 125, 315, 443, 526, 583, 759
BsmAI GTCTC 2 cut(s) 52, 638
BsmBI CGTCTC 1 cut(s) 638
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 9 cut(s) 20, 213, 269, 288, 448, 516, 604, 617, 752
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 3 cut(s) 8, 50, 755
BspACI CCGC 5 cut(s) 33, 138, 209, 386, 561
BspANI GGCC 9 cut(s) 20, 213, 269, 288, 448, 516, 604, 617, 752
BspCNI CTCAG 3 cut(s) 171, 388, 739
BspLI GGNNCC 1 cut(s) 19
BspOI GCTAGC 1 cut(s) 432
BspPI GGATC 2 cut(s) 16, 58
BsrBI CCGCTC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 675
BsrFI RCCGGY 2 cut(s) 134, 284
BsrI ACTGG 2 cut(s) 106, 146
BssAI RCCGGY 2 cut(s) 134, 284
BssECI CCNNGG 3 cut(s) 453, 577, 618
BssMI GATC 3 cut(s) 8, 50, 755
BssT1I CCWWGG 1 cut(s) 577
Bst2UI CCWGG 1 cut(s) 454
Bst4CI ACNGT 1 cut(s) 251
Bst6I CTCTTC 1 cut(s) 726
BstBAI YACGTR 3 cut(s) 346, 425, 728
BstC8I GCNNGC 4 cut(s) 136, 211, 286, 430
BstDEI CTNAG 5 cut(s) 158, 366, 396, 675, 747
BstDSI CCRYGG 1 cut(s) 618
BstENI CCTNNNNNAGG 1 cut(s) 313
BstF5I GGATG 1 cut(s) 449
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 3 cut(s) 11, 53, 758
BstMAI GTCTC 2 cut(s) 52, 638
BstMBI GATC 3 cut(s) 8, 50, 755
BstMWI GCNNNNNNNGC 4 cut(s) 135, 392, 665, 743
BstNI CCWGG 1 cut(s) 454
BstSCI CCNGG 2 cut(s) 443, 452
BstV1I GCAGC 1 cut(s) 379
BstV2I GAAGAC 1 cut(s) 158
BstX2I RGATCY 2 cut(s) 8, 755
BstYI RGATCY 2 cut(s) 8, 755
Bsu36I CCTNAGG 1 cut(s) 158
BsuRI GGCC 9 cut(s) 20, 213, 269, 288, 448, 516, 604, 617, 752
BtgI CCRYGG 1 cut(s) 618
BtsCI GGATG 1 cut(s) 449
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 4 cut(s) 136, 211, 286, 430
CfoI GCGC 1 cut(s) 182
Cfr10I RCCGGY 2 cut(s) 134, 284
Cfr13I GGNCC 5 cut(s) 18, 104, 211, 287, 446
Csp6I GTAC 6 cut(s) 4, 40, 422, 490, 565, 587
CspCI CAANNNNNGTGG 2 cut(s) 687, 722
CviAII CATG 1 cut(s) 22
CviQI GTAC 6 cut(s) 4, 40, 422, 490, 565, 587
DdeI CTNAG 5 cut(s) 158, 366, 396, 675, 747
DpnI GATC 3 cut(s) 10, 52, 757
DpnII GATC 3 cut(s) 8, 50, 755
DriI GACNNNNNGTC 1 cut(s) 685
Eam1104I CTCTTC 1 cut(s) 726
Eam1105I GACNNNNNGTC 1 cut(s) 685
EarI CTCTTC 1 cut(s) 726
EciI GGCGGA 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 577
Eco147I AGGCCT 2 cut(s) 269, 516
Eco47I GGWCC 1 cut(s) 104
Eco72I CACGTG 1 cut(s) 728
Eco81I CCTNAGG 1 cut(s) 158
EcoNI CCTNNNNNAGG 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 452
EcoT14I CCWWGG 1 cut(s) 577
ErhI CCWWGG 1 cut(s) 577
Esp3I CGTCTC 1 cut(s) 638
FaeI CATG 1 cut(s) 25
FaiI YATR 8 cut(s) 23, 132, 293, 332, 411, 417, 548, 705
FalI AAGNNNNNCTT 2 cut(s) 468, 500
FaqI GGGAC 1 cut(s) 332
FatI CATG 1 cut(s) 21
FauI CCCGC 3 cut(s) 26, 202, 554
Fnu4HI GCNGC 1 cut(s) 393
FokI GGATG 1 cut(s) 436
Fsp4HI GCNGC 1 cut(s) 393
FspBI CTAG 3 cut(s) 12, 429, 759
GlaI GCGC 1 cut(s) 181
GluI GCNGC 1 cut(s) 393
HaeIII GGCC 9 cut(s) 20, 213, 269, 288, 448, 516, 604, 617, 752
HapII CCGG 4 cut(s) 135, 285, 358, 444
HhaI GCGC 1 cut(s) 182
Hin1II CATG 1 cut(s) 25
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 1 cut(s) 141
HpaII CCGG 4 cut(s) 135, 285, 358, 444
HphI GGTGA 1 cut(s) 541
Hpy166II GTNNAC 2 cut(s) 492, 532
Hpy188III TCNNGA 1 cut(s) 301
Hpy8I GTNNAC 2 cut(s) 492, 532
HpyAV CCTTC 3 cut(s) 181, 607, 735
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 3 cut(s) 345, 424, 727
HpyCH4V TGCA 3 cut(s) 73, 295, 313
HpyF10VI GCNNNNNNNGC 4 cut(s) 135, 392, 665, 743
HpyF3I CTNAG 5 cut(s) 158, 366, 396, 675, 747
HpySE526I ACGT 3 cut(s) 345, 424, 727
Hsp92II CATG 1 cut(s) 25
HspAI GCGC 1 cut(s) 180
KroI GCCGGC 2 cut(s) 134, 284
KroNI GCCGGC 2 cut(s) 136, 286
Kzo9I GATC 3 cut(s) 8, 50, 755
LmnI GCTCC 2 cut(s) 30, 404
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 2 cut(s) 60, 498
MaeI CTAG 3 cut(s) 12, 429, 759
MaeII ACGT 3 cut(s) 345, 424, 727
MalI GATC 3 cut(s) 10, 52, 757
MbiI CCGCTC 1 cut(s) 33
MboI GATC 3 cut(s) 8, 50, 755
MboII GAAGA 4 cut(s) 158, 187, 517, 743
MflI RGATCY 2 cut(s) 8, 755
MhlI GDGCHC 1 cut(s) 365
MluCI AATT 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 743
MroNI GCCGGC 2 cut(s) 134, 284
MspA1I CMGCKG 1 cut(s) 561
MspI CCGG 4 cut(s) 135, 285, 358, 444
MspR9I CCNGG 2 cut(s) 445, 454
MvaI CCWGG 1 cut(s) 454
MwoI GCNNNNNNNGC 4 cut(s) 135, 392, 665, 743
NaeI GCCGGC 2 cut(s) 136, 286
NciI CCSGG 1 cut(s) 445
NdeII GATC 3 cut(s) 8, 50, 755
NgoMIV GCCGGC 2 cut(s) 134, 284
NheI GCTAGC 1 cut(s) 428
NlaIII CATG 1 cut(s) 25
NlaIV GGNNCC 1 cut(s) 19
PceI AGGCCT 2 cut(s) 269, 516
PdiI GCCGGC 2 cut(s) 136, 286
PfeI GAWTC 1 cut(s) 141
PflMI CCANNNNNTGG 1 cut(s) 125
PkrI GCNGC 1 cut(s) 394
PmaCI CACGTG 1 cut(s) 728
PmlI CACGTG 1 cut(s) 728
Ppu21I YACGTR 3 cut(s) 346, 425, 728
Psp6I CCWGG 1 cut(s) 452
PspCI CACGTG 1 cut(s) 728
PspGI CCWGG 1 cut(s) 452
PspN4I GGNNCC 1 cut(s) 19
PspPI GGNCC 5 cut(s) 18, 104, 211, 287, 446
PsuI RGATCY 2 cut(s) 8, 755
RsaI GTAC 6 cut(s) 5, 41, 423, 491, 566, 588
RsaNI GTAC 6 cut(s) 4, 40, 422, 490, 565, 587
SatI GCNGC 1 cut(s) 393
Sau3AI GATC 3 cut(s) 8, 50, 755
Sau96I GGNCC 5 cut(s) 18, 104, 211, 287, 446
ScrFI CCNGG 2 cut(s) 445, 454
SduI GDGCHC 1 cut(s) 365
SfaNI GCATC 2 cut(s) 60, 498
SinI GGWCC 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 1 cut(s) 254
SseBI AGGCCT 2 cut(s) 269, 516
SsiI CCGC 5 cut(s) 33, 138, 209, 386, 561
SspMI CTAG 3 cut(s) 12, 429, 759
StuI AGGCCT 2 cut(s) 269, 516
StyD4I CCNGG 2 cut(s) 443, 452
StyI CCWWGG 1 cut(s) 577
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 3 cut(s) 348, 427, 730
TaqI TCGA 5 cut(s) 198, 225, 300, 689, 739
TasI AATT 1 cut(s) 254
TatI WGTACW 1 cut(s) 489
TfiI GAWTC 1 cut(s) 141
TscAI CASTG 1 cut(s) 106
TseI GCWGC 1 cut(s) 392
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 1 cut(s) 106
Van91I CCANNNNNTGG 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 104
XagI CCTNNNNNAGG 1 cut(s) 313
XapI RAATTY 1 cut(s) 254
XspI CTAG 3 cut(s) 12, 429, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.