Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014480

Physical Location & Seq

Genomic Coordinates Reverse (-)

26437170 .. 26438771

Gene Structure 1602 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G66140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 753 bp

ATGGCTAGATACGATCGAGCAATTACTGTCTTCTCCCCCGACGGTCACCTCTTTCAAGTCGAATACGCCCTTGAAGCCGTCCGCAAGGGTAACGCCGCCGTCGGTGTCCGCGGTACCGACACCGTTGTCCTCGCCGTCGAGAAGAAGTCCACCCCCAAGCTTCAGGATTCTAGATCAGCCAGAAAAATTGTGAGCCTTGACAATCACATTGCCTTGGCATGCGCGGGGCTCAAGGCTGATGCCCGAGTCTTGATTAACAAAGCAAGGATCGAGTGTCAAAGCCACAGGCTTACACTTGAGGACCCTGTCACTGTTGAGTACATCACTCGCTACATTGCTGGCCTTCAACAGAAGTATACCCAAAGTGGTGGTGTCAGACCCTTCGGTCTTTCTACTCTTATCGTTGGCTTTGACCCTTACTCTCGCCTCCCTTCCCTATATCAGACTGATCCTTCTGGGACTTTCTCTGCTTGGAAAGCTAATGCTACCGGCAGAAACTCCAACTCTATTAGGGAATTCCTCGAGAAGAACTACAAAGAATCCTCTGGCCAAGAAACTATTAAACTCGCTATCCGTGCTCTGCTTGAGGTAGTTGAGAGTGGCGGAAAGAACATTGAGGTTGCCGTAATGACACGGGAGGAAACTGGGCTGCGCCAGCTAGAAGAAGCTGAAATTGATGCAATCGTGGCCAAGATCGAAGCTGAAAAGGCGGCCGCAGAAGCAGCCAAGAAAGGCCCTCCAAAGGAAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005819 GO:0005829 GO:0005839 GO:0005840 GO:0005856 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009507 GO:0009524 GO:0009536 GO:0009987 GO:0015630 GO:0016020 GO:0019538 GO:0019773 GO:0019941 GO:0022626 GO:0030163 GO:0031090 GO:0032991 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0051603 GO:0071704 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03050 map03050

Pfam Domains

Protein Families

Proteasome Proteasome_A_N

Protein Analysis

250

Amino Acids

27.32

Weight (kDa)

8.73

Isoelectric Point (pI)

45.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Proteasome_A_N	PF10584	4 - 26	6e-14	Proteasome subunit A N-terminal signature
Proteasome	PF00227	27 - 210	4.2e-55	Proteasome subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014480)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51260 AT3G51260 AT5G66140
fragaria_vesca	FvH4_1g11520
malus_domestica	MD02G1128200.v1.1 MD15G1243400.v1.1
prunus_persica	Prupe.7G170800_v2.0.a1
pyrus_communis	pycom02g10020 pycom15g21440
rosa_chinensis	RchiOBHm_Chr2g0099151
rosa_laevigata	RLG00000016851
rosa_multiflora	Rmu_sc0012098.1_g000001 Rmu_sc0012711.1_g000003
rosa_roxburghii	Rroxscaffold_2G00143400
rosa_rugosa	Rorug02G0077200
rosa_samantha	Rh2AG126200
rosa_wichuraiana	Rw2G009730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	125, 384
Acc65I	GGTACC	1 cut(s)	113
AccB1I	GGYRCC	1 cut(s)	113
AccI	GTMKAC	1 cut(s)	356
AccII	CGCG	2 cut(s)	111, 224
AciI	CCGC	8 cut(s)	82, 96, 109, 111, 224, 603, 710, 714
AclWI	GGATC	2 cut(s)	275, 443
AcoI	YGGCCR	3 cut(s)	547, 687, 711
AcsI	RAATTY	1 cut(s)	515
AcuI	CTGAAG	1 cut(s)	146
AfaI	GTAC	2 cut(s)	115, 320
AfiI	CCNNNNNNNGG	1 cut(s)	742
AgsI	TTSAA	3 cut(s)	56, 74, 347
AluBI	AGCT	5 cut(s)	160, 479, 658, 668, 701
AluI	AGCT	5 cut(s)	160, 479, 658, 668, 701
Alw21I	GWGCWC	1 cut(s)	580
AlwI	GGATC	2 cut(s)	275, 443
Ama87I	CYCGRG	2 cut(s)	243, 521
AoxI	GGCC	5 cut(s)	340, 547, 687, 711, 733
ApeKI	GCWGC	2 cut(s)	649, 722
ApoI	RAATTY	1 cut(s)	515
Asp718I	GGTACC	1 cut(s)	113
AspLEI	GCGC	2 cut(s)	224, 654
AspS9I	GGNCC	2 cut(s)	301, 734
AsuHPI	GGTGA	1 cut(s)	38
AvaI	CYCGRG	2 cut(s)	243, 521
AvaII	GGWCC	1 cut(s)	301
BalI	TGGCCA	2 cut(s)	549, 689
BanI	GGYRCC	1 cut(s)	113
BanII	GRGCYC	1 cut(s)	231
BbsI	GAAGAC	1 cut(s)	22
Bbv12I	GWGCWC	1 cut(s)	580
BbvI	GCAGC	2 cut(s)	636, 734
BceAI	ACGGC	4 cut(s)	62, 83, 119, 608
BfaI	CTAG	3 cut(s)	6, 171, 659
BisI	GCNGC	5 cut(s)	96, 650, 711, 714, 723
BlsI	GCNGC	5 cut(s)	97, 651, 712, 715, 724
Bme18I	GGWCC	1 cut(s)	301
BmeT110I	CYCGRG	2 cut(s)	243, 521
BmgT120I	GGNCC	2 cut(s)	301, 734
BmiI	GGNNCC	2 cut(s)	115, 303
BmrI	ACTGGG	1 cut(s)	654
BmsI	GCATC	2 cut(s)	229, 667
BmuI	ACTGGG	1 cut(s)	654
BpiI	GAAGAC	1 cut(s)	22
BpuEI	CTTGAG	3 cut(s)	215, 317, 605
BsaJI	CCNNGG	2 cut(s)	109, 213
Bsc4I	CCNNNNNNNGG	1 cut(s)	742
Bse118I	RCCGGY	1 cut(s)	488
Bse1I	ACTGG	1 cut(s)	649
Bse3DI	GCAATG	2 cut(s)	207, 333
BseDI	CCNNGG	2 cut(s)	109, 213
BseLI	CCNNNNNNNGG	1 cut(s)	742
BseMI	GCAATG	2 cut(s)	207, 333
BseNI	ACTGG	1 cut(s)	649
BseX3I	CGGCCG	1 cut(s)	711
BseXI	GCAGC	2 cut(s)	636, 734
Bsh1236I	CGCG	2 cut(s)	111, 224
Bsh1285I	CGRYCG	2 cut(s)	16, 714
BshFI	GGCC	5 cut(s)	342, 549, 689, 713, 735
BshNI	GGYRCC	1 cut(s)	113
BsiEI	CGRYCG	2 cut(s)	16, 714
BsiHKAI	GWGCWC	1 cut(s)	580
BsiHKCI	CYCGRG	2 cut(s)	243, 521
BsiSI	CCGG	1 cut(s)	489
BslFI	GGGAC	1 cut(s)	472
BslI	CCNNNNNNNGG	1 cut(s)	742
BsmFI	GGGAC	1 cut(s)	472
BsnI	GGCC	5 cut(s)	342, 549, 689, 713, 735
BsoBI	CYCGRG	2 cut(s)	243, 521
Bsp1286I	GDGCHC	2 cut(s)	231, 580
Bsp143I	GATC	5 cut(s)	13, 173, 267, 448, 693
BspACI	CCGC	8 cut(s)	82, 96, 109, 111, 224, 603, 710, 714
BspANI	GGCC	5 cut(s)	342, 549, 689, 713, 735
BspFNI	CGCG	2 cut(s)	111, 224
BspLI	GGNNCC	2 cut(s)	115, 303
BspPI	GGATC	2 cut(s)	275, 443
BspT107I	GGYRCC	1 cut(s)	113
BsrDI	GCAATG	2 cut(s)	207, 333
BsrFI	RCCGGY	1 cut(s)	488
BsrI	ACTGG	1 cut(s)	649
BssAI	RCCGGY	1 cut(s)	488
BssECI	CCNNGG	2 cut(s)	109, 213
BssMI	GATC	5 cut(s)	13, 173, 267, 448, 693
BssNAI	GTATAC	1 cut(s)	357
BssT1I	CCWWGG	1 cut(s)	213
Bst1107I	GTATAC	1 cut(s)	357
Bst4CI	ACNGT	4 cut(s)	28, 44, 124, 313
BstC8I	GCNNGC	3 cut(s)	220, 340, 656
BstDSI	CCRYGG	1 cut(s)	109
BstEII	GGTNACC	1 cut(s)	44
BstFNI	CGCG	2 cut(s)	111, 224
BstHHI	GCGC	2 cut(s)	224, 654
BstKTI	GATC	5 cut(s)	16, 176, 270, 451, 696
BstMBI	GATC	5 cut(s)	13, 173, 267, 448, 693
BstMCI	CGRYCG	2 cut(s)	16, 714
BstMWI	GCNNNNNNNGC	8 cut(s)	74, 476, 575, 655, 686, 707, 719, 722
BstNSI	RCATGY	1 cut(s)	222
BstPI	GGTNACC	1 cut(s)	44
BstUI	CGCG	2 cut(s)	111, 224
BstV1I	GCAGC	2 cut(s)	636, 734
BstV2I	GAAGAC	1 cut(s)	22
BstXI	CCANNNNNNTGG	1 cut(s)	368
BstZ17I	GTATAC	1 cut(s)	357
BstZI	CGGCCG	1 cut(s)	711
BsuRI	GGCC	5 cut(s)	342, 549, 689, 713, 735
BtgI	CCRYGG	1 cut(s)	109
BtsIMutI	CAGTG	1 cut(s)	309
Cac8I	GCNNGC	3 cut(s)	220, 340, 656
CciNI	GCGGCCGC	1 cut(s)	711
CfoI	GCGC	2 cut(s)	224, 654
Cfr10I	RCCGGY	1 cut(s)	488
Cfr13I	GGNCC	2 cut(s)	301, 734
Cfr42I	CCGCGG	1 cut(s)	112
Csp6I	GTAC	2 cut(s)	114, 319
CviAII	CATG	1 cut(s)	219
CviQI	GTAC	2 cut(s)	114, 319
DpnI	GATC	5 cut(s)	15, 175, 269, 450, 695
DpnII	GATC	5 cut(s)	13, 173, 267, 448, 693
DrdI	GACNNNNNNGTC	2 cut(s)	125, 384
DseDI	GACNNNNNNGTC	2 cut(s)	125, 384
EaeI	YGGCCR	3 cut(s)	547, 687, 711
EagI	CGGCCG	1 cut(s)	711
EciI	GGCGGA	1 cut(s)	618
EclXI	CGGCCG	1 cut(s)	711
Eco130I	CCWWGG	1 cut(s)	213
Eco24I	GRGCYC	1 cut(s)	231
Eco47I	GGWCC	1 cut(s)	301
Eco52I	CGGCCG	1 cut(s)	711
Eco57I	CTGAAG	1 cut(s)	146
Eco88I	CYCGRG	2 cut(s)	243, 521
Eco91I	GGTNACC	1 cut(s)	44
EcoO109I	RGGNCCY	2 cut(s)	301, 734
EcoO65I	GGTNACC	1 cut(s)	44
EcoRI	GAATTC	1 cut(s)	515
EcoT14I	CCWWGG	1 cut(s)	213
EcoT38I	GRGCYC	1 cut(s)	231
ErhI	CCWWGG	1 cut(s)	213
FaeI	CATG	1 cut(s)	222
FaiI	YATR	3 cut(s)	220, 357, 439
FaqI	GGGAC	1 cut(s)	472
FatI	CATG	1 cut(s)	218
FauI	CCCGC	1 cut(s)	217
FblI	GTMKAC	1 cut(s)	356
Fnu4HI	GCNGC	5 cut(s)	96, 650, 711, 714, 723
FriOI	GRGCYC	1 cut(s)	231
Fsp4HI	GCNGC	5 cut(s)	96, 650, 711, 714, 723
FspBI	CTAG	3 cut(s)	6, 171, 659
GlaI	GCGC	2 cut(s)	223, 653
GluI	GCNGC	5 cut(s)	96, 650, 711, 714, 723
HaeIII	GGCC	5 cut(s)	342, 549, 689, 713, 735
HapII	CCGG	1 cut(s)	489
HhaI	GCGC	2 cut(s)	224, 654
Hin1II	CATG	1 cut(s)	222
Hin6I	GCGC	2 cut(s)	222, 652
HinP1I	GCGC	2 cut(s)	222, 652
HindIII	AAGCTT	1 cut(s)	158
HinfI	GANTC	3 cut(s)	167, 246, 539
HpaII	CCGG	1 cut(s)	489
HphI	GGTGA	1 cut(s)	38
Hpy166II	GTNNAC	2 cut(s)	150, 357
Hpy188I	TCNGA	2 cut(s)	377, 444
Hpy188III	TCNNGA	5 cut(s)	139, 164, 171, 250, 523
Hpy8I	GTNNAC	2 cut(s)	150, 357
Hpy99I	CGWCG	3 cut(s)	44, 104, 140
HpyAV	CCTTC	4 cut(s)	353, 391, 441, 462
HpyCH4III	ACNGT	4 cut(s)	28, 44, 124, 313
HpyCH4V	TGCA	1 cut(s)	680
HpyF10VI	GCNNNNNNNGC	8 cut(s)	74, 476, 575, 655, 686, 707, 719, 722
Hsp92II	CATG	1 cut(s)	222
HspAI	GCGC	2 cut(s)	222, 652
KpnI	GGTACC	1 cut(s)	117
KspI	CCGCGG	1 cut(s)	112
Kzo9I	GATC	5 cut(s)	13, 173, 267, 448, 693
Lsp1109I	GCAGC	2 cut(s)	636, 734
LweI	GCATC	2 cut(s)	229, 667
MaeI	CTAG	3 cut(s)	6, 171, 659
MaeIII	GTNAC	3 cut(s)	44, 89, 307
MalI	GATC	5 cut(s)	15, 175, 269, 450, 695
MboI	GATC	5 cut(s)	13, 173, 267, 448, 693
MboII	GAAGA	4 cut(s)	22, 154, 538, 674
MhlI	GDGCHC	2 cut(s)	231, 580
MlsI	TGGCCA	2 cut(s)	549, 689
MluCI	AATT	4 cut(s)	21, 186, 515, 672
MluNI	TGGCCA	2 cut(s)	549, 689
MlyI	GAGTC	1 cut(s)	255
MmeI	TCCRAC	1 cut(s)	525
Mox20I	TGGCCA	2 cut(s)	549, 689
MscI	TGGCCA	2 cut(s)	549, 689
MseI	TTAA	2 cut(s)	255, 561
Msp20I	TGGCCA	2 cut(s)	549, 689
MspA1I	CMGCKG	1 cut(s)	111
MspI	CCGG	1 cut(s)	489
MvnI	CGCG	2 cut(s)	111, 224
MwoI	GCNNNNNNNGC	8 cut(s)	74, 476, 575, 655, 686, 707, 719, 722
NdeII	GATC	5 cut(s)	13, 173, 267, 448, 693
NlaIII	CATG	1 cut(s)	222
NlaIV	GGNNCC	2 cut(s)	115, 303
NmuCI	GTSAC	2 cut(s)	44, 307
NotI	GCGGCCGC	1 cut(s)	711
NspI	RCATGY	1 cut(s)	222
PaeI	GCATGC	1 cut(s)	222
PaeR7I	CTCGAG	1 cut(s)	521
PfeI	GAWTC	2 cut(s)	167, 539
PflFI	GACNNNGTC	1 cut(s)	305
PkrI	GCNGC	5 cut(s)	97, 651, 712, 715, 724
Ple19I	CGATCG	1 cut(s)	16
PleI	GAGTC	1 cut(s)	254
PpsI	GAGTC	1 cut(s)	254
PpuMI	RGGWCCY	1 cut(s)	301
Psp5II	RGGWCCY	1 cut(s)	301
PspEI	GGTNACC	1 cut(s)	44
PspN4I	GGNNCC	2 cut(s)	115, 303
PspPI	GGNCC	2 cut(s)	301, 734
PspPPI	RGGWCCY	1 cut(s)	301
PsyI	GACNNNGTC	1 cut(s)	305
PvuI	CGATCG	1 cut(s)	16
RsaI	GTAC	2 cut(s)	115, 320
RsaNI	GTAC	2 cut(s)	114, 319
SacII	CCGCGG	1 cut(s)	112
SaqAI	TTAA	2 cut(s)	255, 561
SatI	GCNGC	5 cut(s)	96, 650, 711, 714, 723
Sau3AI	GATC	5 cut(s)	13, 173, 267, 448, 693
Sau96I	GGNCC	2 cut(s)	301, 734
SchI	GAGTC	1 cut(s)	255
SduI	GDGCHC	2 cut(s)	231, 580
SetI	ASST	8 cut(s)	51, 162, 481, 591, 621, 660, 670, 703
SfaNI	GCATC	2 cut(s)	229, 667
Sfr274I	CTCGAG	1 cut(s)	521
Sfr303I	CCGCGG	1 cut(s)	112
SgrBI	CCGCGG	1 cut(s)	112
SinI	GGWCC	1 cut(s)	301
SlaI	CTCGAG	1 cut(s)	521
SmlI	CTYRAG	4 cut(s)	230, 296, 521, 584
SmoI	CTYRAG	4 cut(s)	230, 296, 521, 584
SphI	GCATGC	1 cut(s)	222
Sse9I	AATT	4 cut(s)	21, 186, 515, 672
SsiI	CCGC	8 cut(s)	82, 96, 109, 111, 224, 603, 710, 714
SspMI	CTAG	3 cut(s)	6, 171, 659
StyI	CCWWGG	1 cut(s)	213
TaaI	ACNGT	4 cut(s)	28, 44, 124, 313
TaqI	TCGA	6 cut(s)	16, 60, 138, 270, 522, 696
TaqII	GACCGA	1 cut(s)	374
TasI	AATT	4 cut(s)	21, 186, 515, 672
TatI	WGTACW	1 cut(s)	318
TauI	GCSGC	3 cut(s)	98, 713, 716
TfiI	GAWTC	2 cut(s)	167, 539
Tru1I	TTAA	2 cut(s)	255, 561
Tru9I	TTAA	2 cut(s)	255, 561
TscAI	CASTG	1 cut(s)	316
TseFI	GTSAC	2 cut(s)	44, 307
TseI	GCWGC	2 cut(s)	649, 722
Tsp45I	GTSAC	2 cut(s)	44, 307
TspGWI	ACGGA	1 cut(s)	563
TspRI	CASTG	1 cut(s)	316
Tth111I	GACNNNGTC	1 cut(s)	305
VpaK11BI	GGWCC	1 cut(s)	301
XapI	RAATTY	1 cut(s)	515
XbaI	TCTAGA	1 cut(s)	170
XceI	RCATGY	1 cut(s)	222
XhoI	CTCGAG	1 cut(s)	521
XmiI	GTMKAC	1 cut(s)	356
XspI	CTAG	3 cut(s)	6, 171, 659

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.