AT5G66520

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016684
Physical Location & Seq
Forward (+)
26551784 .. 26553741
1958 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66520.1

Sequence Viewer

Length: 1863 bp
ATGAATGTGATCTCATGCTCCTTCTCATTGGAACATAATCTCTACGAGACAATGTCTTGTCTCCAGAGATGCTCAAAGCAAGAAGAACTGAAGCAAATCCACGCTCGCATGCTGAAAACTGGCTTGATGCAGGATTCTTATGCAATCACAAAGTTTCTTTCTTTCTGCATTTCTTCAACGTCTTCCGACTTTTTGCCTTATGCCCAGATTGTGTTTGACGGGTTTGATCGACCAGATACTTTCTTGTGGAACCTAATGATCAGAGGGTTCTCGTGCTCAGACGAACCCGAGAGGTCTCTTCTCCTGTATCAACGTATGCTCTGTTCTTCAGCTCCTCATAACGCGTATACTTTTCCGTCTCTTCTCAAAGCTTGTTCGAACCTATCTGCATTTGAAGAAACAACGCAAATTCACGCACAGATCACGAAACTTGGATATGAAAATGATGTCTATGCAGTGAATTCTCTGATTAATTCATATGCTGTGACCGGGAATTTCAAGCTAGCTCACCTTCTCTTTGACAGAATCCCCGAACCTGATGATGTCTCGTGGAACTCTGTGATCAAAGGTTATGTAAAAGCTGGAAAAATGGATATTGCATTAACGTTATTCAGGAAAATGGCAGAAAAGAATGCTATATCATGGACTACGATGATCTCTGGGTATGTTCAAGCAGACATGAACAAGGAAGCTCTGCAATTGTTTCATGAAATGCAGAATTCAGATGTTGAGCCTGATAATGTTTCCCTAGCTAATGCTCTCTCAGCTTGTGCTCAGCTCGGAGCACTCGAGCAAGGGAAATGGATCCATTCCTATCTTAATAAGACAAGAATCAGAATGGACTCTGTTTTGGGTTGTGTTCTTATAGATATGTATGCAAAGTGCGGTGAAATGGAAGAAGCTTTGGAAGTTTTCAAGAATATTAAGAAAAAATCAGTGCAAGCATGGACAGCTTTGATTTCAGGATATGCATACCATGGCCATGGAAGAGAAGCTATTAGCAAATTCATGGAGATGCAAAAGATGGGAATTAAGCCAAATGTGATCACTTTCACTGCGGTTCTCACGGCTTGCAGCTACACAGGACTAGTTGAAGAAGGAAAGTTGATATTCTACAGCATGGAGAGAGATTACAACCTGAAACCGACCATCGAGCATTATGGCTGTATTGTTGATTTACTCGGTCGAGCTGGATTGCTTGATGAAGCAAAACGTTTCATTCAGGAGATGCCATTGAAGCCAAATGCTGTGATATGGGGTGCGTTGCTCAAAGCCTGTCGAATTCACAAAAACATCGAATTGGGAGAGGAAATTGGAGAAATCTTAATTGCAATAGATCCATATCATGGCGGAAGATATGTTCATAAGGCAAATATTCATGCTATGGATAAAAAGTGGGACAAAGCAGCTGAAACAAGAAGATTGATGAAAGAACAAGGAGTAGCAAAAGTTCCAGGATGTAGTACAATTAGCTTGGAAGGGACCACACACGAGTTTTTAGCGGGAGACCGATCACACCCAGAGATAGAGAAAATTCAATCTAAATGGAGAATCATGAGAAGGAAACTTGAGGAAAACGGGTACGTACCAGAGTTAGAAGAGATGCTGCTTGATCTAGTTGATGATGATGAAAGAGAGGCTATTGTGCATCAGCATAGCGAGAAATTGGCTATTACATACGGGTTAATCAAGACTAAACCAGGAACCATTATTCGTATAATGAAGAATCTCCGAGTATGCAAAGATTGTCACAAAGTAACGAAGCTCATCTCTAAGATATACAAGAGGGATATTGTAATGAGAGATAGGACAAGGTTCCATCATTTCAGAGATGGGAAATGCAGTTGTGGGGACTACTGGTAA

Protein Analysis

620

Amino Acids

70.72

Weight (kDa)

7.53

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 178 - 207 2.2e-06 PPR repeat
PPR_2 PF13041 179 - 211 3.4e-07 PPR repeat family
PPR PF01535 182 - 210 8.5e-08 PPR repeat
PPR_2 PF13041 211 - 258 5.7e-10 PPR repeat family
PPR PF01535 213 - 242 1.2e-07 PPR repeat
PPR_2 PF13041 312 - 358 6.8e-12 PPR repeat family
PPR_3 PF13812 313 - 358 6.2e-08 Pentatricopeptide repeat domain
PPR_3 PF13812 337 - 395 1.1e-06 Pentatricopeptide repeat domain
E_motif PF20431 429 - 490 1.3e-11 E motif
DYW_deaminase PF14432 527 - 620 6e-41 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016684)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G02980 AT5G66520
fragaria_vesca FvH4_4g31300
malus_domestica MD13G1071700.v1.1
prunus_persica Prupe.1G275200_v2.0.a1
pyrus_communis pycom13g06430
rosa_chinensis RchiOBHm_Chr4g0439791
rosa_laevigata RLG00000006238
rosa_multiflora Rmu_sc0010226.1_g000006 Rmu_ssc0000029.1_g000006
rosa_roxburghii Rroxscaffold_5G00380690
rosa_rugosa Rorug04G0321400
rosa_samantha Rh4BG384700
rosa_wichuraiana Rw4G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1346
AccI GTMKAC 1 cut(s) 347
AccII CGCG 1 cut(s) 344
AciI CCGC 4 cut(s) 885, 1058, 1350, 1502
AclI AACGTT 2 cut(s) 605, 1213
AclWI GGATC 3 cut(s) 799, 812, 1331
AcoI YGGCCR 1 cut(s) 979
AcsI RAATTY 7 cut(s) 408, 460, 493, 718, 1004, 1281, 1533
AcuI CTGAAG 2 cut(s) 110, 312
AfaI GTAC 3 cut(s) 1465, 1583, 1587
AfiI CCNNNNNNNGG 1 cut(s) 1346
AflIII ACRYGT 1 cut(s) 342
AgsI TTSAA 8 cut(s) 177, 395, 499, 671, 916, 1094, 1237, 1538
AhlI ACTAGT 1 cut(s) 1087
AjnI CCWGG 2 cut(s) 1453, 1699
AloI GAACNNNNNNTCC 2 cut(s) 1344, 1376
Alw21I GWGCWC 3 cut(s) 278, 775, 787
Alw26I GTCTC 6 cut(s) 41, 65, 300, 363, 550, 1500
AlwI GGATC 3 cut(s) 799, 812, 1331
Ama87I CYCGRG 2 cut(s) 287, 788
AoxI GGCC 1 cut(s) 979
ApeKI GCWGC 3 cut(s) 1074, 1406, 1606
ApoI RAATTY 7 cut(s) 408, 460, 493, 718, 1004, 1281, 1533
AseI ATTAAT 1 cut(s) 471
AspS9I GGNCC 1 cut(s) 1482
AsuC2I CCSGG 1 cut(s) 490
AsuHPI GGTGA 2 cut(s) 500, 899
AsuII TTCGAA 1 cut(s) 377
AsuNHI GCTAGC 1 cut(s) 502
AvaI CYCGRG 2 cut(s) 287, 788
AvaII GGWCC 1 cut(s) 1482
BalI TGGCCA 1 cut(s) 981
BamHI GGATCC 1 cut(s) 804
BauI CACGAG 3 cut(s) 271, 547, 1490
BbsI GAAGAC 1 cut(s) 174
Bbv12I GWGCWC 3 cut(s) 278, 775, 787
BbvI GCAGC 3 cut(s) 1086, 1418, 1593
BccI CCATC 4 cut(s) 1018, 1157, 1826, 1827
BceAI ACGGC 1 cut(s) 1083
BciT130I CCWGG 2 cut(s) 1455, 1701
BclI TGATCA 3 cut(s) 258, 561, 1044
BcnI CCSGG 1 cut(s) 490
BcoDI GTCTC 6 cut(s) 41, 65, 300, 363, 550, 1500
BcuI ACTAGT 1 cut(s) 1087
BfaI CTAG 4 cut(s) 503, 749, 1088, 1616
BfmI CTRYAG 1 cut(s) 1114
BisI GCNGC 3 cut(s) 1075, 1407, 1607
BlpI GCTNAGC 1 cut(s) 774
BlsI GCNGC 3 cut(s) 1076, 1408, 1608
Bme1390I CCNGG 3 cut(s) 490, 1455, 1701
Bme18I GGWCC 1 cut(s) 1482
BmeT110I CYCGRG 2 cut(s) 287, 788
BmgT120I GGNCC 1 cut(s) 1482
BmiI GGNNCC 5 cut(s) 251, 806, 1483, 1705, 1817
BmrFI CCNGG 3 cut(s) 490, 1455, 1701
BmsI GCATC 6 cut(s) 59, 117, 1005, 1218, 1593, 1657
BmtI GCTAGC 1 cut(s) 506
BpiI GAAGAC 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 47
Bpu1102I GCTNAGC 1 cut(s) 774
Bpu14I TTCGAA 1 cut(s) 377
BpuEI CTTGAG 1 cut(s) 1589
BpuMI CCSGG 1 cut(s) 490
BsaAI YACGTR 1 cut(s) 1585
BsaBI GATNNNNATC 1 cut(s) 1341
BsaI GGTCTC 2 cut(s) 300, 1500
BsaJI CCNNGG 2 cut(s) 976, 982
Bsc4I CCNNNNNNNGG 1 cut(s) 1346
Bse1I ACTGG 2 cut(s) 124, 1862
Bse8I GATNNNNATC 1 cut(s) 1341
BseBI CCWGG 2 cut(s) 1455, 1701
BseDI CCNNGG 2 cut(s) 976, 982
BseGI GGATG 1 cut(s) 1463
BseJI GATNNNNATC 1 cut(s) 1341
BseLI CCNNNNNNNGG 1 cut(s) 1346
BseMII CTCAG 3 cut(s) 291, 777, 788
BseNI ACTGG 2 cut(s) 124, 1862
BseRI GAGGAG 1 cut(s) 324
BseXI GCAGC 3 cut(s) 1086, 1418, 1593
Bsh1236I CGCG 1 cut(s) 344
Bsh1285I CGRYCG 1 cut(s) 1186
BshFI GGCC 1 cut(s) 981
BsiEI CGRYCG 1 cut(s) 1186
BsiHKAI GWGCWC 3 cut(s) 278, 775, 787
BsiHKCI CYCGRG 2 cut(s) 287, 788
BsiSI CCGG 1 cut(s) 489
BslFI GGGAC 2 cut(s) 1412, 1495
BslI CCNNNNNNNGG 1 cut(s) 1346
BsmAI GTCTC 6 cut(s) 41, 65, 300, 363, 550, 1500
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 2 cut(s) 1412, 1495
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 1 cut(s) 981
Bso31I GGTCTC 2 cut(s) 300, 1500
BsoBI CYCGRG 2 cut(s) 287, 788
Bsp119I TTCGAA 1 cut(s) 377
Bsp1286I GDGCHC 3 cut(s) 278, 775, 787
Bsp1720I GCTNAGC 1 cut(s) 774
Bsp19I CCATGG 2 cut(s) 976, 982
BspACI CCGC 4 cut(s) 885, 1058, 1350, 1502
BspANI GGCC 1 cut(s) 981
BspCNI CTCAG 3 cut(s) 290, 776, 787
BspFNI CGCG 1 cut(s) 344
BspHI TCATGA 2 cut(s) 706, 1554
BspLI GGNNCC 5 cut(s) 251, 806, 1483, 1705, 1817
BspOI GCTAGC 1 cut(s) 506
BspPI GGATC 3 cut(s) 799, 812, 1331
BspT104I TTCGAA 1 cut(s) 377
BspTNI GGTCTC 2 cut(s) 300, 1500
BsrI ACTGG 2 cut(s) 124, 1862
BssECI CCNNGG 2 cut(s) 976, 982
BssNAI GTATAC 1 cut(s) 348
BssSI CACGAG 3 cut(s) 271, 547, 1490
BssT1I CCWWGG 2 cut(s) 976, 982
Bst1107I GTATAC 1 cut(s) 348
Bst2BI CACGAG 3 cut(s) 271, 547, 1490
Bst2UI CCWGG 2 cut(s) 1455, 1701
Bst6I CTCTTC 4 cut(s) 303, 366, 982, 1593
BstBAI YACGTR 1 cut(s) 1585
BstBI TTCGAA 1 cut(s) 377
BstC8I GCNNGC 5 cut(s) 106, 110, 504, 942, 1072
BstDEI CTNAG 4 cut(s) 277, 763, 774, 1773
BstDSI CCRYGG 2 cut(s) 976, 982
BstF5I GGATG 1 cut(s) 1463
BstFNI CGCG 1 cut(s) 344
BstMAI GTCTC 6 cut(s) 41, 65, 300, 363, 550, 1500
BstMCI CGRYCG 1 cut(s) 1186
BstMWI GCNNNNNNNGC 3 cut(s) 764, 950, 1237
BstNI CCWGG 2 cut(s) 1455, 1701
BstNSI RCATGY 1 cut(s) 112
BstSCI CCNGG 3 cut(s) 488, 1453, 1699
BstSFI CTRYAG 1 cut(s) 1114
BstSNI TACGTA 1 cut(s) 1585
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 3 cut(s) 1086, 1418, 1593
BstV2I GAAGAC 1 cut(s) 174
BstX2I RGATCY 2 cut(s) 804, 1336
BstXI CCANNNNNNTGG 1 cut(s) 983
BstYI RGATCY 2 cut(s) 804, 1336
BstZ17I GTATAC 1 cut(s) 348
BsuRI GGCC 1 cut(s) 981
BtgI CCRYGG 2 cut(s) 976, 982
BtsCI GGATG 1 cut(s) 1463
BtsI GCAGTG 2 cut(s) 462, 1053
BtsIMutI CAGTG 3 cut(s) 462, 942, 1053
Cac8I GCNNGC 5 cut(s) 106, 110, 504, 942, 1072
CciI TCATGA 2 cut(s) 706, 1554
Cfr13I GGNCC 1 cut(s) 1482
Csp6I GTAC 3 cut(s) 1464, 1582, 1586
CviQI GTAC 3 cut(s) 1464, 1582, 1586
DdeI CTNAG 4 cut(s) 277, 763, 774, 1773
EaeI YGGCCR 1 cut(s) 979
Eam1104I CTCTTC 4 cut(s) 303, 366, 982, 1593
EarI CTCTTC 4 cut(s) 303, 366, 982, 1593
EciI GGCGGA 1 cut(s) 1365
Eco105I TACGTA 1 cut(s) 1585
Eco130I CCWWGG 2 cut(s) 976, 982
Eco31I GGTCTC 2 cut(s) 300, 1500
Eco47I GGWCC 1 cut(s) 1482
Eco57I CTGAAG 2 cut(s) 110, 312
Eco88I CYCGRG 2 cut(s) 287, 788
EcoRI GAATTC 3 cut(s) 460, 718, 1281
EcoRII CCWGG 2 cut(s) 1453, 1699
EcoT14I CCWWGG 2 cut(s) 976, 982
EcoT22I ATGCAT 1 cut(s) 973
ErhI CCWWGG 2 cut(s) 976, 982
Esp3I CGTCTC 1 cut(s) 363
FaqI GGGAC 2 cut(s) 1412, 1495
FauI CCCGC 1 cut(s) 1495
FauNDI CATATG 1 cut(s) 478
FbaI TGATCA 3 cut(s) 258, 561, 1044
FblI GTMKAC 1 cut(s) 347
Fnu4HI GCNGC 3 cut(s) 1075, 1407, 1607
FokI GGATG 1 cut(s) 1470
Fsp4HI GCNGC 3 cut(s) 1075, 1407, 1607
FspBI CTAG 4 cut(s) 503, 749, 1088, 1616
GluI GCNGC 3 cut(s) 1075, 1407, 1607
GsuI CTGGAG 1 cut(s) 47
HaeIII GGCC 1 cut(s) 981
HapII CCGG 1 cut(s) 489
HindIII AAGCTT 2 cut(s) 369, 900
HinfI GANTC 6 cut(s) 134, 525, 831, 842, 1551, 1726
HpaII CCGG 1 cut(s) 489
HphI GGTGA 2 cut(s) 500, 899
Hpy166II GTNNAC 1 cut(s) 348
Hpy188I TCNGA 9 cut(s) 187, 263, 280, 468, 724, 782, 836, 1733, 1829
Hpy188III TCNNGA 9 cut(s) 64, 424, 613, 707, 916, 963, 1223, 1555, 1690
Hpy8I GTNNAC 1 cut(s) 348
HpyAV CCTTC 5 cut(s) 31, 521, 1091, 1472, 1554
HpyCH4IV ACGT 5 cut(s) 179, 313, 605, 1213, 1584
HpyF10VI GCNNNNNNNGC 3 cut(s) 764, 950, 1237
HpyF3I CTNAG 4 cut(s) 277, 763, 774, 1773
HpySE526I ACGT 5 cut(s) 179, 313, 605, 1213, 1584
Ksp22I TGATCA 3 cut(s) 258, 561, 1044
LmnI GCTCC 3 cut(s) 23, 337, 782
Lsp1109I GCAGC 3 cut(s) 1086, 1418, 1593
LweI GCATC 6 cut(s) 59, 117, 1005, 1218, 1593, 1657
MaeI CTAG 4 cut(s) 503, 749, 1088, 1616
MaeII ACGT 5 cut(s) 179, 313, 605, 1213, 1584
MaeIII GTNAC 3 cut(s) 484, 1748, 1756
MfeI CAATTG 1 cut(s) 698
MflI RGATCY 2 cut(s) 804, 1336
MhlI GDGCHC 3 cut(s) 278, 775, 787
MlsI TGGCCA 1 cut(s) 981
MluI ACGCGT 1 cut(s) 342
MluNI TGGCCA 1 cut(s) 981
MlyI GAGTC 1 cut(s) 836
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 7 cut(s) 257, 285, 345, 1300, 1564, 1630, 1779
Mox20I TGGCCA 1 cut(s) 981
Mph1103I ATGCAT 1 cut(s) 973
MscI TGGCCA 1 cut(s) 981
MseI TTAA 7 cut(s) 471, 602, 819, 924, 1032, 1325, 1685
MslI CAYNNNNRTG 2 cut(s) 981, 1013
Msp20I TGGCCA 1 cut(s) 981
MspA1I CMGCKG 1 cut(s) 1409
MspI CCGG 1 cut(s) 489
MspR9I CCNGG 3 cut(s) 490, 1455, 1701
MunI CAATTG 1 cut(s) 698
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 2 cut(s) 1455, 1701
MvnI CGCG 1 cut(s) 344
MwoI GCNNNNNNNGC 3 cut(s) 764, 950, 1237
NciI CCSGG 1 cut(s) 490
NcoI CCATGG 2 cut(s) 976, 982
NdeI CATATG 1 cut(s) 478
NheI GCTAGC 1 cut(s) 502
NlaIV GGNNCC 5 cut(s) 251, 806, 1483, 1705, 1817
NmuCI GTSAC 2 cut(s) 484, 1748
NsiI ATGCAT 1 cut(s) 973
NspI RCATGY 1 cut(s) 112
NspV TTCGAA 1 cut(s) 377
PaeI GCATGC 1 cut(s) 112
PaeR7I CTCGAG 1 cut(s) 788
PagI TCATGA 2 cut(s) 706, 1554
PcsI WCGNNNNNNNCGW 1 cut(s) 786
PctI GAATGC 1 cut(s) 637
PfeI GAWTC 5 cut(s) 134, 525, 831, 1551, 1726
PflFI GACNNNGTC 1 cut(s) 52
PflMI CCANNNNNTGG 1 cut(s) 1346
PfoI TCCNGGA 1 cut(s) 1453
PkrI GCNGC 3 cut(s) 1076, 1408, 1608
PleI GAGTC 1 cut(s) 836
PpsI GAGTC 1 cut(s) 836
Ppu21I YACGTR 1 cut(s) 1585
PshBI ATTAAT 1 cut(s) 471
Psp1406I AACGTT 2 cut(s) 605, 1213
Psp6I CCWGG 2 cut(s) 1453, 1699
PspGI CCWGG 2 cut(s) 1453, 1699
PspN4I GGNNCC 5 cut(s) 251, 806, 1483, 1705, 1817
PspPI GGNCC 1 cut(s) 1482
PspXI VCTCGAGB 1 cut(s) 788
PsrI GAACNNNNNNTAC 4 cut(s) 307, 339, 1434, 1466
PsuI RGATCY 2 cut(s) 804, 1336
PsyI GACNNNGTC 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 1409
RsaI GTAC 3 cut(s) 1465, 1583, 1587
RsaNI GTAC 3 cut(s) 1464, 1582, 1586
RseI CAYNNNNRTG 2 cut(s) 981, 1013
SaqAI TTAA 7 cut(s) 471, 602, 819, 924, 1032, 1325, 1685
SatI GCNGC 3 cut(s) 1075, 1407, 1607
Sau96I GGNCC 1 cut(s) 1482
SchI GAGTC 1 cut(s) 836
ScrFI CCNGG 3 cut(s) 490, 1455, 1701
SduI GDGCHC 3 cut(s) 278, 775, 787
SfaNI GCATC 6 cut(s) 59, 117, 1005, 1218, 1593, 1657
SfcI CTRYAG 1 cut(s) 1114
Sfr274I CTCGAG 1 cut(s) 788
SfuI TTCGAA 1 cut(s) 377
SinI GGWCC 1 cut(s) 1482
SlaI CTCGAG 1 cut(s) 788
SmiMI CAYNNNNRTG 2 cut(s) 981, 1013
SmlI CTYRAG 2 cut(s) 788, 1568
SmoI CTYRAG 2 cut(s) 788, 1568
SnaBI TACGTA 1 cut(s) 1585
SpeI ACTAGT 1 cut(s) 1087
SphI GCATGC 1 cut(s) 112
SsiI CCGC 4 cut(s) 885, 1058, 1350, 1502
SspI AATATT 2 cut(s) 922, 1375
SspMI CTAG 4 cut(s) 503, 749, 1088, 1616
StyD4I CCNGG 3 cut(s) 488, 1453, 1699
StyI CCWWGG 2 cut(s) 976, 982
TaiI ACGT 5 cut(s) 182, 316, 608, 1216, 1587
TaqI TCGA 7 cut(s) 229, 377, 789, 1152, 1186, 1279, 1296
TaqII GACCGA 2 cut(s) 1172, 1524
TatI WGTACW 1 cut(s) 1463
TfiI GAWTC 5 cut(s) 134, 525, 831, 1551, 1726
Tru1I TTAA 7 cut(s) 471, 602, 819, 924, 1032, 1325, 1685
Tru9I TTAA 7 cut(s) 471, 602, 819, 924, 1032, 1325, 1685
TscAI CASTG 3 cut(s) 462, 942, 1060
TseFI GTSAC 2 cut(s) 484, 1748
TseI GCWGC 3 cut(s) 1074, 1406, 1606
Tsp45I GTSAC 2 cut(s) 484, 1748
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 3 cut(s) 462, 942, 1060
Tth111I GACNNNGTC 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 1346
VpaK11BI GGWCC 1 cut(s) 1482
VspI ATTAAT 1 cut(s) 471
XapI RAATTY 7 cut(s) 408, 460, 493, 718, 1004, 1281, 1533
XceI RCATGY 1 cut(s) 112
XhoI CTCGAG 1 cut(s) 788
XmiI GTMKAC 1 cut(s) 347
XspI CTAG 4 cut(s) 503, 749, 1088, 1616
Zsp2I ATGCAT 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.