AT5G66740

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012891
Physical Location & Seq
Forward (+)
26646900 .. 26648731
1832 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66740.1

Sequence Viewer

Length: 1113 bp
ATGAGGAAGCTGTGTCCGAACATAGACAAAGACGATGGTCTGGAGACGGTGTTGGAAGTTCCGATACCGGAGGAGATGTTTTCCGGTATGGGCAACAACGTTGCACTTAGGTGGCAAAATATGATGACGTGGATGAAAGCTCAAACGTCTGATAAATGGTCGCAACCGCTTATCGCCGCTCGTATCAACGAGCTCCGGTTCCTTCTCTACCTCGTTGGCTCGCCTCTTATACCTCTCCAGGTTCAAGTCGGTCACTCTGTTCATAAGCCCGTCAAAGATTGCTCCATTCAAGCTTCAACGGCGAAATACATTGTACAGCAGTACATAGCAGCGACGGGAGGACCACAGGCGTTAAACGCCGTGAACAGCATGTGCGTCACGGGACAAGTGAAGATGACGGCGTCGGAGTTTCATCAAGGAGATGATTCGGGCGTTAATCTAAAGAGCAACGACGAAATGGGTGGTTTCGTTTTATGGCAAAAGGATCCAGATCTTTGGTGTTTGGAGCTCGTCGTCTCCGGTTGCAAAGTGATATGTGGAAGCAACGGTCGGCTTTCATGGCGACATTCCTCTAACCAGCAAACTCCGGCGTCTACCGGAACGCCAAGACCTCTCCGCCGGTTTTTACAGGGGTTAGATCCTCGTTCGACGGCGAATCTGTTTCTTGACGCAACGTGTATCGGAGAGAAGATAATCAACGGTGAGGATTGCTTTATCTTGAAACTGGAGACGAGTCCGGCGGTTCGAGAAGCTCAAAGCGGTCCGAATTTTGAGATAATTCATCACACGATATGGGGTTATTTTAGTCAAAGATCGGGACTTTTGATTCAGTTCGAAGATTCGCGGCTTTTGAGAATGAGGACCAAGGAAGACGAAGATGTCTTCTGGGAGACTAGTGCTGAGTCGGTGATGGATGATTACCGATACGTTGACAATGTGAACATCGCTCACGGCGGGAAAACATCGGTCACGGTTTTCCGGTACGGTGAAGCGTCGGCGAATCATCGGAGACAGATGACGGAGAAGTGGAGGATAGAAGAAGTTGATTTTAATGTTTGGGGTCTCTCCGTTGATCATTTTCTTCCTCCGGCCAATTTGCAGATCGAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

370

Amino Acids

41.68

Weight (kDa)

5.76

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 124 - 363 1.3e-117 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012891)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66740
fragaria_vesca FvH4_1g13560
malus_domestica MD02G1150900.v1.1 MD15G1265400.v1.1
prunus_persica Prupe.7G152200_v2.0.a1
pyrus_communis pycom02g11850 pycom15g23110
rosa_chinensis RchiOBHm_Chr2g0101971
rosa_laevigata RLG00000017120
rosa_multiflora Rmu_co8388187.1_g000001
rosa_roxburghii Rroxscaffold_2G00140880
rosa_rugosa Rorug02G0103400 Rorug02G0103500
rosa_samantha Rh2AG151600 Rh2BG157000 Rh2CG157000 Rh2DG157100
rosa_wichuraiana Rw2G011830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 878
AccBSI CCGCTC 1 cut(s) 179
AccI GTMKAC 1 cut(s) 593
AccII CGCG 1 cut(s) 844
AciI CCGC 7 cut(s) 167, 177, 616, 740, 759, 844, 954
AclI AACGTT 1 cut(s) 99
AclWI GGATC 3 cut(s) 479, 492, 632
AcoI YGGCCR 1 cut(s) 1089
AcsI RAATTY 1 cut(s) 766
AcyI GRCGYC 2 cut(s) 401, 590
AfaI GTAC 3 cut(s) 315, 323, 983
AflIII ACRYGT 1 cut(s) 674
AgsI TTSAA 4 cut(s) 245, 290, 297, 721
AhlI ACTAGT 1 cut(s) 893
AjiI CACGTC 1 cut(s) 129
AjnI CCWGG 1 cut(s) 237
AleI CACNNNNGTG 1 cut(s) 109
AluBI AGCT 6 cut(s) 10, 140, 193, 293, 508, 752
AluI AGCT 6 cut(s) 10, 140, 193, 293, 508, 752
Alw21I GWGCWC 2 cut(s) 195, 510
Alw26I GTCTC 6 cut(s) 38, 520, 722, 884, 1003, 1067
AlwI GGATC 3 cut(s) 479, 492, 632
AoxI GGCC 1 cut(s) 1089
ApeKI GCWGC 1 cut(s) 329
ApoI RAATTY 1 cut(s) 766
AspS9I GGNCC 3 cut(s) 341, 761, 861
AsuHPI GGTGA 3 cut(s) 713, 919, 998
AsuII TTCGAA 1 cut(s) 834
AvaII GGWCC 3 cut(s) 341, 761, 861
BamHI GGATCC 1 cut(s) 484
BanII GRGCYC 2 cut(s) 195, 510
BarI GAAGNNNNNNTAC 2 cut(s) 48, 80
BbsI GAAGAC 2 cut(s) 874, 876
Bbv12I GWGCWC 2 cut(s) 195, 510
BbvI GCAGC 1 cut(s) 341
BccI CCATC 2 cut(s) 29, 904
BceAI ACGGC 5 cut(s) 315, 344, 414, 666, 967
BciT130I CCWGG 1 cut(s) 239
BclI TGATCA 1 cut(s) 1072
BcoDI GTCTC 6 cut(s) 38, 520, 722, 884, 1003, 1067
BcuI ACTAGT 1 cut(s) 893
BfaI CTAG 1 cut(s) 894
BglII AGATCT 1 cut(s) 490
BisI GCNGC 3 cut(s) 177, 330, 845
BlsI GCNGC 3 cut(s) 178, 331, 846
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 3 cut(s) 341, 761, 861
BmgBI CACGTC 1 cut(s) 129
BmgT120I GGNCC 3 cut(s) 341, 761, 861
BmiI GGNNCC 2 cut(s) 200, 486
BmrFI CCNGG 1 cut(s) 239
BoxI GACNNNNGTC 1 cut(s) 36
BpiI GAAGAC 2 cut(s) 874, 876
BpmI CTGGAG 3 cut(s) 62, 221, 746
Bpu14I TTCGAA 1 cut(s) 834
BsaBI GATNNNNATC 1 cut(s) 489
BsaHI GRCGYC 2 cut(s) 401, 590
BsaI GGTCTC 1 cut(s) 1067
BsaJI CCNNGG 1 cut(s) 864
BsaWI WCCGGW 6 cut(s) 67, 83, 195, 518, 596, 978
BsaXI ACNNNNNCTCC 4 cut(s) 35, 62, 65, 92
Bse118I RCCGGY 1 cut(s) 618
Bse1I ACTGG 1 cut(s) 729
Bse8I GATNNNNATC 1 cut(s) 489
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 864
BseGI GGATG 2 cut(s) 138, 919
BseJI GATNNNNATC 1 cut(s) 489
BseMII CTCAG 1 cut(s) 891
BseNI ACTGG 1 cut(s) 729
BseRI GAGGAG 1 cut(s) 86
BseXI GCAGC 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 844
Bsh1285I CGRYCG 1 cut(s) 550
BshFI GGCC 1 cut(s) 1091
BsiEI CGRYCG 1 cut(s) 550
BsiHKAI GWGCWC 2 cut(s) 195, 510
BslFI GGGAC 2 cut(s) 396, 831
BsmAI GTCTC 6 cut(s) 38, 520, 722, 884, 1003, 1067
BsmBI CGTCTC 3 cut(s) 38, 520, 722
BsmFI GGGAC 2 cut(s) 396, 831
BsnI GGCC 1 cut(s) 1091
Bso31I GGTCTC 1 cut(s) 1067
Bsp119I TTCGAA 1 cut(s) 834
Bsp1286I GDGCHC 2 cut(s) 195, 510
Bsp1407I TGTACA 1 cut(s) 313
Bsp143I GATC 6 cut(s) 484, 490, 637, 812, 1072, 1101
BspACI CCGC 7 cut(s) 167, 177, 616, 740, 759, 844, 954
BspANI GGCC 1 cut(s) 1091
BspCNI CTCAG 1 cut(s) 892
BspFNI CGCG 1 cut(s) 844
BspLI GGNNCC 2 cut(s) 200, 486
BspPI GGATC 3 cut(s) 479, 492, 632
BspT104I TTCGAA 1 cut(s) 834
BspTNI GGTCTC 1 cut(s) 1067
BsrBI CCGCTC 1 cut(s) 179
BsrFI RCCGGY 1 cut(s) 618
BsrGI TGTACA 1 cut(s) 313
BsrI ACTGG 1 cut(s) 729
BssAI RCCGGY 1 cut(s) 618
BssECI CCNNGG 1 cut(s) 864
BssMI GATC 6 cut(s) 484, 490, 637, 812, 1072, 1101
BssNI GRCGYC 2 cut(s) 401, 590
BssT1I CCWWGG 1 cut(s) 864
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 5 cut(s) 49, 548, 701, 973, 986
BstACI GRCGYC 2 cut(s) 401, 590
BstAUI TGTACA 1 cut(s) 313
BstBI TTCGAA 1 cut(s) 834
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 2 cut(s) 107, 900
BstF5I GGATG 2 cut(s) 138, 919
BstFNI CGCG 1 cut(s) 844
BstKTI GATC 6 cut(s) 487, 493, 640, 815, 1075, 1104
BstMAI GTCTC 6 cut(s) 38, 520, 722, 884, 1003, 1067
BstMBI GATC 6 cut(s) 484, 490, 637, 812, 1072, 1101
BstMCI CGRYCG 1 cut(s) 550
BstMWI GCNNNNNNNGC 3 cut(s) 299, 356, 559
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 1 cut(s) 373
BstPAI GACNNNNGTC 1 cut(s) 36
BstSCI CCNGG 1 cut(s) 237
BstUI CGCG 1 cut(s) 844
BstV1I GCAGC 1 cut(s) 341
BstV2I GAAGAC 2 cut(s) 874, 876
BstX2I RGATCY 3 cut(s) 484, 490, 637
BstXI CCANNNNNNTGG 1 cut(s) 495
BstYI RGATCY 3 cut(s) 484, 490, 637
BsuRI GGCC 1 cut(s) 1091
BtgZI GCGATG 1 cut(s) 928
BtrI CACGTC 1 cut(s) 129
BtsCI GGATG 2 cut(s) 138, 919
Cac8I GCNNGC 1 cut(s) 221
Cfr10I RCCGGY 1 cut(s) 618
Cfr13I GGNCC 3 cut(s) 341, 761, 861
CpoI CGGWCCG 1 cut(s) 761
CseI GACGC 5 cut(s) 364, 390, 579, 677, 981
Csp6I GTAC 3 cut(s) 314, 322, 982
CspI CGGWCCG 1 cut(s) 761
CviAII CATG 2 cut(s) 370, 558
CviQI GTAC 3 cut(s) 314, 322, 982
DdeI CTNAG 2 cut(s) 107, 900
DpnI GATC 6 cut(s) 486, 492, 639, 814, 1074, 1103
DpnII GATC 6 cut(s) 484, 490, 637, 812, 1072, 1101
DrdI GACNNNNNNGTC 1 cut(s) 878
DseDI GACNNNNNNGTC 1 cut(s) 878
EaeI YGGCCR 1 cut(s) 1089
EciI GGCGGA 1 cut(s) 605
Ecl136II GAGCTC 2 cut(s) 193, 508
Eco130I CCWWGG 1 cut(s) 864
Eco24I GRGCYC 2 cut(s) 195, 510
Eco31I GGTCTC 1 cut(s) 1067
Eco47I GGWCC 3 cut(s) 341, 761, 861
Eco53kI GAGCTC 2 cut(s) 193, 508
EcoICRI GAGCTC 2 cut(s) 193, 508
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 1 cut(s) 864
EcoT38I GRGCYC 2 cut(s) 195, 510
ErhI CCWWGG 1 cut(s) 864
Esp3I CGTCTC 3 cut(s) 38, 520, 722
FaeI CATG 2 cut(s) 373, 561
FaqI GGGAC 2 cut(s) 396, 831
FatI CATG 2 cut(s) 369, 557
FauI CCCGC 1 cut(s) 947
FbaI TGATCA 1 cut(s) 1072
FblI GTMKAC 1 cut(s) 593
Fnu4HI GCNGC 3 cut(s) 177, 330, 845
FokI GGATG 2 cut(s) 145, 926
FriOI GRGCYC 2 cut(s) 195, 510
Fsp4HI GCNGC 3 cut(s) 177, 330, 845
FspBI CTAG 1 cut(s) 894
GluI GCNGC 3 cut(s) 177, 330, 845
GsuI CTGGAG 3 cut(s) 62, 221, 746
HaeIII GGCC 1 cut(s) 1091
HgaI GACGC 5 cut(s) 364, 390, 579, 677, 981
Hin1I GRCGYC 2 cut(s) 401, 590
Hin1II CATG 2 cut(s) 373, 561
HincII GTYRAC 1 cut(s) 931
HindII GTYRAC 1 cut(s) 931
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 7 cut(s) 425, 655, 733, 826, 839, 902, 1000
HphI GGTGA 3 cut(s) 713, 919, 998
Hpy166II GTNNAC 4 cut(s) 364, 594, 931, 940
Hpy188I TCNGA 7 cut(s) 18, 63, 151, 406, 683, 765, 1008
Hpy188III TCNNGA 6 cut(s) 41, 488, 665, 718, 746, 816
Hpy8I GTNNAC 4 cut(s) 364, 594, 931, 940
Hpy99I CGWCG 6 cut(s) 337, 406, 455, 515, 652, 997
HpyAV CCTTC 1 cut(s) 212
HpyCH4III ACNGT 5 cut(s) 49, 548, 701, 973, 986
HpyCH4IV ACGT 5 cut(s) 99, 128, 146, 674, 927
HpyCH4V TGCA 3 cut(s) 104, 525, 1099
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 356, 559
HpyF3I CTNAG 2 cut(s) 107, 900
HpySE526I ACGT 5 cut(s) 99, 128, 146, 674, 927
Hsp92I GRCGYC 2 cut(s) 401, 590
Hsp92II CATG 2 cut(s) 373, 561
Ksp22I TGATCA 1 cut(s) 1072
Kzo9I GATC 6 cut(s) 484, 490, 637, 812, 1072, 1101
LmnI GCTCC 3 cut(s) 198, 287, 505
Lsp1109I GCAGC 1 cut(s) 341
MaeI CTAG 1 cut(s) 894
MaeII ACGT 5 cut(s) 99, 128, 146, 674, 927
MaeIII GTNAC 3 cut(s) 251, 376, 967
MalI GATC 6 cut(s) 486, 492, 639, 814, 1074, 1103
MbiI CCGCTC 1 cut(s) 179
MboI GATC 6 cut(s) 484, 490, 637, 812, 1072, 1101
MboII GAAGA 8 cut(s) 403, 700, 848, 874, 881, 887, 1049, 1073
MflI RGATCY 3 cut(s) 484, 490, 637
MhlI GDGCHC 2 cut(s) 195, 510
MluCI AATT 3 cut(s) 766, 777, 1093
MlyI GAGTC 2 cut(s) 742, 911
MmeI TCCRAC 2 cut(s) 33, 384
MseI TTAA 3 cut(s) 353, 435, 1050
MslI CAYNNNNRTG 1 cut(s) 109
MspR9I CCNGG 1 cut(s) 239
MvaI CCWGG 1 cut(s) 239
MvnI CGCG 1 cut(s) 844
MwoI GCNNNNNNNGC 3 cut(s) 299, 356, 559
NdeII GATC 6 cut(s) 484, 490, 637, 812, 1072, 1101
NlaIII CATG 2 cut(s) 373, 561
NlaIV GGNNCC 2 cut(s) 200, 486
NmuCI GTSAC 3 cut(s) 251, 376, 967
NspI RCATGY 1 cut(s) 373
NspV TTCGAA 1 cut(s) 834
OliI CACNNNNGTG 1 cut(s) 109
PfeI GAWTC 5 cut(s) 425, 655, 826, 839, 1000
PflFI GACNNNGTC 1 cut(s) 400
PkrI GCNGC 3 cut(s) 178, 331, 846
PleI GAGTC 2 cut(s) 741, 910
PpsI GAGTC 2 cut(s) 741, 910
PshAI GACNNNNGTC 1 cut(s) 36
Psp124BI GAGCTC 2 cut(s) 195, 510
Psp1406I AACGTT 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 2 cut(s) 200, 486
PspPI GGNCC 3 cut(s) 341, 761, 861
PsuI RGATCY 3 cut(s) 484, 490, 637
PsyI GACNNNGTC 1 cut(s) 400
RsaI GTAC 3 cut(s) 315, 323, 983
RsaNI GTAC 3 cut(s) 314, 322, 982
RseI CAYNNNNRTG 1 cut(s) 109
Rsr2I CGGWCCG 1 cut(s) 761
RsrII CGGWCCG 1 cut(s) 761
SacI GAGCTC 2 cut(s) 195, 510
SaqAI TTAA 3 cut(s) 353, 435, 1050
SatI GCNGC 3 cut(s) 177, 330, 845
Sau3AI GATC 6 cut(s) 484, 490, 637, 812, 1072, 1101
Sau96I GGNCC 3 cut(s) 341, 761, 861
SchI GAGTC 2 cut(s) 742, 911
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 2 cut(s) 195, 510
SfuI TTCGAA 1 cut(s) 834
SinI GGWCC 3 cut(s) 341, 761, 861
SmiMI CAYNNNNRTG 1 cut(s) 109
SpeI ACTAGT 1 cut(s) 893
Sse9I AATT 3 cut(s) 766, 777, 1093
SsiI CCGC 7 cut(s) 167, 177, 616, 740, 759, 844, 954
SspMI CTAG 1 cut(s) 894
SstI GAGCTC 2 cut(s) 195, 510
StyD4I CCNGG 1 cut(s) 237
StyI CCWWGG 1 cut(s) 864
TaaI ACNGT 5 cut(s) 49, 548, 701, 973, 986
TaiI ACGT 5 cut(s) 102, 131, 149, 677, 930
TaqI TCGA 4 cut(s) 647, 745, 834, 1104
TaqII GACCGA 2 cut(s) 239, 955
TasI AATT 3 cut(s) 766, 777, 1093
TatI WGTACW 2 cut(s) 313, 321
TauI GCSGC 2 cut(s) 179, 847
TfiI GAWTC 5 cut(s) 425, 655, 826, 839, 1000
Tru1I TTAA 3 cut(s) 353, 435, 1050
Tru9I TTAA 3 cut(s) 353, 435, 1050
TseFI GTSAC 3 cut(s) 251, 376, 967
TseI GCWGC 1 cut(s) 329
Tsp45I GTSAC 3 cut(s) 251, 376, 967
TspDTI ATGAA 5 cut(s) 149, 251, 401, 546, 770
TspGWI ACGGA 2 cut(s) 1034, 1057
Tth111I GACNNNGTC 1 cut(s) 400
VpaK11BI GGWCC 3 cut(s) 341, 761, 861
XapI RAATTY 1 cut(s) 766
XceI RCATGY 1 cut(s) 373
XmiI GTMKAC 1 cut(s) 593
XspI CTAG 1 cut(s) 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.