Prupe.7G152200_v2.0.a1

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0012891
Physical Location & Seq
Forward (+)
16281874 .. 16283873
2000 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G152200.1

Sequence Viewer

Length: 1116 bp
ATGAGGAAGCTTTGTCCAAATATTGATAAAGAAGATGGCCTTGAGACTGTTCTTGAGGTCCCTATACCAGAAGAGATGTTCACAAGCATGGGAAGCAATGTGCAGTTGCGATTGCAAAACATGATGGCATGGATGAAGGCTCAAACCTCCGACAAATGGTCGGCGCCAGTCATCGCGGGACGGTTGAATGAGCTACGGTTCTTGCTTTACCTTGTAGGCTCTCCTCTTATCCCTCTTCAAGTTCAGTTGGGTCATTCCATCCACCGTCCAGTTAGAGATTCTTCCATTCAAGCTTCAACAGCCAAATACATAGTGCAACAGTACACCGCTGCAACGGGAGGCCAAGCAGCATTGAACTCGGTGAACAGCATGTGTGTGACAGGCGAGGTTAAGATCAGTGCGTCGGATTTTCAACAAGGGGACGTGAGCCTGGAGGTGAAAAGGAGCTCGGCCGAGAGCGGCGGCTTCTTGCTGTGGCAAAAGAACCCGGACTTATGGTGCTTAGAGCTTGTTGTTTCTGGCTGCAAGGTCATCTGTGGAAGCAATGGCAAGCTTTCATGGCGGCATTCCTCTAACCAACAAACACCCATCTCTCAAGGCCCCCCTAGACCCTTGCGCCGTTTTCTTCAGGGTTTGGACCCAAGGGCCACGGCCAATTTATTCGCGGATGCAATGTGCATCGGCGAAAAGATCATCAACGACGACGACTGCTTCATCCTCAAGCTGGACACAAGCCCAGCAATTCGAGAGGCACAGAGCGGCCCAAACTACGAGATAATCCACCACACAATATGGGGATACTTCAGCCAGCGCTCGGGGCTCCTCGTACAATTCGAAGACTCGAGGCTGCTGAGGATGACCAACAAAGCGGACGACACAGATGTGTTCTGGGAGACGAGCGCTGAGTCTGTGATACAAGATTACAAGTACGTTGACTCCGTCAACATTGCTCACAGTGGGAGAACTAGGGTCAAGGTTTTCAGATATGGCGAGCGGTCTGCAAACCACAAGAGGGAGATGGAGGAGACGTGGAAGATCGAAGAGGTGGATTTTAATATTTGGGGTTTGACCATGGAGAGCTTTTTGCCTCCTGCTGACATAAAGAAGCAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

372

Amino Acids

41.71

Weight (kDa)

6.03

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012891)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66740
fragaria_vesca FvH4_1g13560
malus_domestica MD02G1150900.v1.1 MD15G1265400.v1.1
prunus_persica Prupe.7G152200_v2.0.a1
pyrus_communis pycom02g11850 pycom15g23110
rosa_chinensis RchiOBHm_Chr2g0101971
rosa_laevigata RLG00000017120
rosa_multiflora Rmu_co8388187.1_g000001
rosa_roxburghii Rroxscaffold_2G00140880
rosa_rugosa Rorug02G0103400 Rorug02G0103500
rosa_samantha Rh2AG151600 Rh2BG157000 Rh2CG157000 Rh2DG157100
rosa_wichuraiana Rw2G011830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 163
AccBSI CCGCTC 3 cut(s) 459, 759, 994
AccII CGCG 2 cut(s) 176, 665
AciI CCGC 9 cut(s) 176, 327, 459, 462, 562, 665, 759, 869, 994
AcoI YGGCCR 2 cut(s) 450, 651
AcuI CTGAAG 2 cut(s) 611, 787
AcyI GRCGYC 1 cut(s) 164
AfaI GTAC 3 cut(s) 323, 828, 929
AfeI AGCGCT 2 cut(s) 812, 901
AfiI CCNNNNNNNGG 4 cut(s) 156, 724, 814, 1012
AgsI TTSAA 6 cut(s) 187, 239, 290, 297, 355, 413
AhdI GACNNNNNGTC 1 cut(s) 157
AjiI CACGTC 2 cut(s) 424, 1029
AjnI CCWGG 1 cut(s) 429
AleI CACNNNNGTG 1 cut(s) 881
AluBI AGCT 8 cut(s) 10, 193, 293, 447, 508, 553, 724, 1080
AluI AGCT 8 cut(s) 10, 193, 293, 447, 508, 553, 724, 1080
Alw21I GWGCWC 1 cut(s) 449
Alw26I GTCTC 3 cut(s) 38, 887, 1019
Ama87I CYCGRG 2 cut(s) 814, 841
Aor51HI AGCGCT 2 cut(s) 812, 901
AoxI GGCC 7 cut(s) 37, 340, 450, 598, 645, 651, 760
ApeKI GCWGC 4 cut(s) 329, 347, 522, 847
AspLEI GCGC 4 cut(s) 166, 618, 813, 902
AspS9I GGNCC 5 cut(s) 58, 599, 637, 645, 761
AsuC2I CCSGG 1 cut(s) 488
AsuHPI GGTGA 2 cut(s) 373, 448
AsuII TTCGAA 1 cut(s) 834
AvaI CYCGRG 2 cut(s) 814, 841
AvaII GGWCC 2 cut(s) 58, 637
BanI GGYRCC 1 cut(s) 163
BanII GRGCYC 2 cut(s) 449, 822
BbsI GAAGAC 1 cut(s) 843
Bbv12I GWGCWC 1 cut(s) 449
BbvCI CCTCAGC 1 cut(s) 851
BbvI GCAGC 4 cut(s) 316, 359, 509, 834
BccI CCATC 5 cut(s) 29, 118, 266, 596, 1012
BceAI ACGGC 2 cut(s) 603, 666
BcgI CGANNNNNNTGC 4 cut(s) 339, 373, 980, 1014
BciT130I CCWGG 1 cut(s) 431
BciVI GTATCC 1 cut(s) 791
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 3 cut(s) 38, 887, 1019
BfaI CTAG 2 cut(s) 606, 966
BfoI RGCGCY 3 cut(s) 167, 814, 903
BfuI GTATCC 1 cut(s) 791
BisI GCNGC 8 cut(s) 330, 348, 460, 463, 523, 563, 760, 848
BlsI GCNGC 8 cut(s) 331, 349, 461, 464, 524, 564, 761, 849
Bme1390I CCNGG 2 cut(s) 431, 488
Bme18I GGWCC 2 cut(s) 58, 637
BmeRI GACNNNNNGTC 1 cut(s) 157
BmeT110I CYCGRG 2 cut(s) 814, 841
BmgBI CACGTC 2 cut(s) 424, 1029
BmgT120I GGNCC 5 cut(s) 58, 599, 637, 645, 761
BmiI GGNNCC 5 cut(s) 60, 165, 601, 639, 821
BmrFI CCNGG 2 cut(s) 431, 488
BmsI GCATC 2 cut(s) 658, 687
BpiI GAAGAC 1 cut(s) 843
BpmI CTGGAG 1 cut(s) 452
Bpu10I CCTNAGC 1 cut(s) 851
Bpu14I TTCGAA 1 cut(s) 834
BpuEI CTTGAG 4 cut(s) 62, 74, 579, 704
BpuMI CCSGG 1 cut(s) 488
BsaHI GRCGYC 1 cut(s) 164
BsaJI CCNNGG 3 cut(s) 641, 648, 1071
BsaXI ACNNNNNCTCC 4 cut(s) 920, 950, 1013, 1043
Bsc4I CCNNNNNNNGG 4 cut(s) 156, 724, 814, 1012
Bse1I ACTGG 2 cut(s) 167, 269
Bse3DI GCAATG 4 cut(s) 103, 550, 678, 945
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 3 cut(s) 641, 648, 1071
BseGI GGATG 5 cut(s) 138, 258, 673, 714, 861
BseLI CCNNNNNNNGG 4 cut(s) 156, 724, 814, 1012
BseMI GCAATG 4 cut(s) 103, 550, 678, 945
BseMII CTCAG 2 cut(s) 842, 894
BseNI ACTGG 2 cut(s) 167, 269
BseRI GAGGAG 3 cut(s) 213, 812, 1037
BseX3I CGGCCG 1 cut(s) 450
BseXI GCAGC 4 cut(s) 316, 359, 509, 834
BseYI CCCAGC 1 cut(s) 736
BsgI GTGCAG 1 cut(s) 122
Bsh1236I CGCG 2 cut(s) 176, 665
Bsh1285I CGRYCG 1 cut(s) 453
BshFI GGCC 7 cut(s) 39, 342, 452, 600, 647, 653, 762
BshNI GGYRCC 1 cut(s) 163
BsiEI CGRYCG 1 cut(s) 453
BsiHKAI GWGCWC 1 cut(s) 449
BsiHKCI CYCGRG 2 cut(s) 814, 841
BsiSI CCGG 1 cut(s) 488
BslFI GGGAC 3 cut(s) 44, 192, 434
BslI CCNNNNNNNGG 4 cut(s) 156, 724, 814, 1012
BsmAI GTCTC 3 cut(s) 38, 887, 1019
BsmBI CGTCTC 2 cut(s) 887, 1019
BsmFI GGGAC 3 cut(s) 44, 192, 434
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 7 cut(s) 39, 342, 452, 600, 647, 653, 762
BsoBI CYCGRG 2 cut(s) 814, 841
Bsp119I TTCGAA 1 cut(s) 834
Bsp1286I GDGCHC 2 cut(s) 449, 822
Bsp143I GATC 3 cut(s) 393, 690, 1035
Bsp19I CCATGG 1 cut(s) 1071
BspACI CCGC 9 cut(s) 176, 327, 459, 462, 562, 665, 759, 869, 994
BspANI GGCC 7 cut(s) 39, 342, 452, 600, 647, 653, 762
BspCNI CTCAG 2 cut(s) 843, 895
BspFNI CGCG 2 cut(s) 176, 665
BspLI GGNNCC 5 cut(s) 60, 165, 601, 639, 821
BspT104I TTCGAA 1 cut(s) 834
BspT107I GGYRCC 1 cut(s) 163
BsrBI CCGCTC 3 cut(s) 459, 759, 994
BsrDI GCAATG 4 cut(s) 103, 550, 678, 945
BsrI ACTGG 2 cut(s) 167, 269
BssECI CCNNGG 3 cut(s) 641, 648, 1071
BssMI GATC 3 cut(s) 393, 690, 1035
BssNI GRCGYC 1 cut(s) 164
BssT1I CCWWGG 2 cut(s) 641, 1071
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 6 cut(s) 49, 183, 198, 266, 321, 956
Bst6I CTCTTC 3 cut(s) 66, 240, 1035
BstACI GRCGYC 1 cut(s) 164
BstBI TTCGAA 1 cut(s) 834
BstC8I GCNNGC 3 cut(s) 551, 809, 992
BstDEI CTNAG 3 cut(s) 502, 851, 903
BstDSI CCRYGG 2 cut(s) 648, 1071
BstF5I GGATG 5 cut(s) 138, 258, 673, 714, 861
BstFNI CGCG 2 cut(s) 176, 665
BstH2I RGCGCY 3 cut(s) 167, 814, 903
BstHHI GCGC 4 cut(s) 166, 618, 813, 902
BstKTI GATC 3 cut(s) 396, 693, 1038
BstMAI GTCTC 3 cut(s) 38, 887, 1019
BstMBI GATC 3 cut(s) 393, 690, 1035
BstMCI CGRYCG 1 cut(s) 453
BstMWI GCNNNNNNNGC 4 cut(s) 93, 299, 559, 817
BstNI CCWGG 1 cut(s) 431
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 2 cut(s) 429, 486
BstUI CGCG 2 cut(s) 176, 665
BstV1I GCAGC 4 cut(s) 316, 359, 509, 834
BstV2I GAAGAC 1 cut(s) 843
BstZI CGGCCG 1 cut(s) 450
BsuI GTATCC 1 cut(s) 791
BsuRI GGCC 7 cut(s) 39, 342, 452, 600, 647, 653, 762
BtgI CCRYGG 2 cut(s) 648, 1071
BtgZI GCGATG 1 cut(s) 157
BtrI CACGTC 2 cut(s) 424, 1029
BtsCI GGATG 5 cut(s) 138, 258, 673, 714, 861
BtsIMutI CAGTG 2 cut(s) 403, 961
Cac8I GCNNGC 3 cut(s) 551, 809, 992
CfoI GCGC 4 cut(s) 166, 618, 813, 902
Cfr13I GGNCC 5 cut(s) 58, 599, 637, 645, 761
CseI GACGC 1 cut(s) 390
Csp6I GTAC 3 cut(s) 322, 827, 928
CviAII CATG 6 cut(s) 88, 121, 129, 370, 558, 1072
CviQI GTAC 3 cut(s) 322, 827, 928
DdeI CTNAG 3 cut(s) 502, 851, 903
DinI GGCGCC 1 cut(s) 165
DpnI GATC 3 cut(s) 395, 692, 1037
DpnII GATC 3 cut(s) 393, 690, 1035
DriI GACNNNNNGTC 1 cut(s) 157
EaeI YGGCCR 2 cut(s) 450, 651
EagI CGGCCG 1 cut(s) 450
Eam1104I CTCTTC 3 cut(s) 66, 240, 1035
Eam1105I GACNNNNNGTC 1 cut(s) 157
EarI CTCTTC 3 cut(s) 66, 240, 1035
Ecl136II GAGCTC 1 cut(s) 447
EclXI CGGCCG 1 cut(s) 450
Eco130I CCWWGG 2 cut(s) 641, 1071
Eco24I GRGCYC 2 cut(s) 449, 822
Eco47I GGWCC 2 cut(s) 58, 637
Eco47III AGCGCT 2 cut(s) 812, 901
Eco52I CGGCCG 1 cut(s) 450
Eco53kI GAGCTC 1 cut(s) 447
Eco57I CTGAAG 2 cut(s) 611, 787
Eco88I CYCGRG 2 cut(s) 814, 841
EcoICRI GAGCTC 1 cut(s) 447
EcoO109I RGGNCCY 2 cut(s) 58, 599
EcoRII CCWGG 1 cut(s) 429
EcoT14I CCWWGG 2 cut(s) 641, 1071
EcoT38I GRGCYC 2 cut(s) 449, 822
EgeI GGCGCC 1 cut(s) 165
EheI GGCGCC 1 cut(s) 165
ErhI CCWWGG 2 cut(s) 641, 1071
Esp3I CGTCTC 2 cut(s) 887, 1019
FaeI CATG 6 cut(s) 91, 124, 132, 373, 561, 1075
FalI AAGNNNNNCTT 2 cut(s) 24, 56
FaqI GGGAC 3 cut(s) 44, 192, 434
FatI CATG 6 cut(s) 87, 120, 128, 369, 557, 1071
FauI CCCGC 1 cut(s) 169
Fnu4HI GCNGC 8 cut(s) 330, 348, 460, 463, 523, 563, 760, 848
FokI GGATG 5 cut(s) 145, 245, 680, 701, 868
FriOI GRGCYC 2 cut(s) 449, 822
Fsp4HI GCNGC 8 cut(s) 330, 348, 460, 463, 523, 563, 760, 848
FspBI CTAG 2 cut(s) 606, 966
GlaI GCGC 4 cut(s) 165, 617, 812, 901
GluI GCNGC 8 cut(s) 330, 348, 460, 463, 523, 563, 760, 848
GsaI CCCAGC 1 cut(s) 740
GsuI CTGGAG 1 cut(s) 452
HaeII RGCGCY 3 cut(s) 167, 814, 903
HaeIII GGCC 7 cut(s) 39, 342, 452, 600, 647, 653, 762
HapII CCGG 1 cut(s) 488
HgaI GACGC 1 cut(s) 390
HhaI GCGC 4 cut(s) 166, 618, 813, 902
Hin1I GRCGYC 1 cut(s) 164
Hin1II CATG 6 cut(s) 91, 124, 132, 373, 561, 1075
Hin6I GCGC 4 cut(s) 164, 616, 811, 900
HinP1I GCGC 4 cut(s) 164, 616, 811, 900
HincII GTYRAC 2 cut(s) 934, 943
HindII GTYRAC 2 cut(s) 934, 943
HindIII AAGCTT 3 cut(s) 8, 291, 551
HinfI GANTC 4 cut(s) 278, 839, 905, 935
HpaII CCGG 1 cut(s) 488
HphI GGTGA 2 cut(s) 373, 448
Hpy166II GTNNAC 5 cut(s) 81, 324, 364, 934, 943
Hpy188I TCNGA 3 cut(s) 151, 406, 983
Hpy188III TCNNGA 2 cut(s) 53, 746
Hpy8I GTNNAC 5 cut(s) 81, 324, 364, 934, 943
Hpy99I CGWCG 3 cut(s) 406, 704, 707
HpyAV CCTTC 1 cut(s) 130
HpyCH4III ACNGT 6 cut(s) 49, 183, 198, 266, 321, 956
HpyCH4IV ACGT 3 cut(s) 423, 930, 1028
HpyCH4V TGCA 8 cut(s) 103, 115, 316, 332, 525, 671, 678, 1001
HpyF10VI GCNNNNNNNGC 4 cut(s) 93, 299, 559, 817
HpyF3I CTNAG 3 cut(s) 502, 851, 903
HpySE526I ACGT 3 cut(s) 423, 930, 1028
Hsp92I GRCGYC 1 cut(s) 164
Hsp92II CATG 6 cut(s) 91, 124, 132, 373, 561, 1075
HspAI GCGC 4 cut(s) 164, 616, 811, 900
KasI GGCGCC 1 cut(s) 163
Kzo9I GATC 3 cut(s) 393, 690, 1035
LmnI GCTCC 2 cut(s) 444, 825
Lsp1109I GCAGC 4 cut(s) 316, 359, 509, 834
LweI GCATC 2 cut(s) 658, 687
MaeI CTAG 2 cut(s) 606, 966
MaeII ACGT 3 cut(s) 423, 930, 1028
MaeIII GTNAC 1 cut(s) 376
MalI GATC 3 cut(s) 395, 692, 1037
MbiI CCGCTC 3 cut(s) 459, 759, 994
MboI GATC 3 cut(s) 393, 690, 1035
MboII GAAGA 8 cut(s) 44, 83, 227, 273, 617, 848, 1045, 1052
MhlI GDGCHC 2 cut(s) 449, 822
MluCI AATT 3 cut(s) 655, 741, 830
Mly113I GGCGCC 1 cut(s) 164
MlyI GAGTC 3 cut(s) 833, 914, 929
MmeI TCCRAC 2 cut(s) 174, 384
MseI TTAA 2 cut(s) 390, 1053
MslI CAYNNNNRTG 3 cut(s) 86, 374, 881
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 1 cut(s) 488
MspR9I CCNGG 2 cut(s) 431, 488
Mva1269I GAATGC 1 cut(s) 565
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 2 cut(s) 176, 665
MwoI GCNNNNNNNGC 4 cut(s) 93, 299, 559, 817
NarI GGCGCC 1 cut(s) 164
NciI CCSGG 1 cut(s) 488
NcoI CCATGG 1 cut(s) 1071
NdeII GATC 3 cut(s) 393, 690, 1035
NlaIII CATG 6 cut(s) 91, 124, 132, 373, 561, 1075
NlaIV GGNNCC 5 cut(s) 60, 165, 601, 639, 821
NmeAIII GCCGAG 2 cut(s) 428, 478
NmuCI GTSAC 1 cut(s) 376
NspI RCATGY 1 cut(s) 373
NspV TTCGAA 1 cut(s) 834
OliI CACNNNNGTG 1 cut(s) 881
PaeR7I CTCGAG 1 cut(s) 841
PcsI WCGNNNNNNNCGW 2 cut(s) 831, 936
PctI GAATGC 1 cut(s) 565
PfeI GAWTC 1 cut(s) 278
PflFI GACNNNGTC 1 cut(s) 938
PkrI GCNGC 8 cut(s) 331, 349, 461, 464, 524, 564, 761, 849
PleI GAGTC 3 cut(s) 833, 913, 929
PluTI GGCGCC 1 cut(s) 167
PpsI GAGTC 3 cut(s) 833, 913, 929
PpuMI RGGWCCY 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 58
Psp6I CCWGG 1 cut(s) 429
PspFI CCCAGC 1 cut(s) 736
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 5 cut(s) 60, 165, 601, 639, 821
PspPI GGNCC 5 cut(s) 58, 599, 637, 645, 761
PspPPI RGGWCCY 1 cut(s) 58
PspXI VCTCGAGB 1 cut(s) 841
PsyI GACNNNGTC 1 cut(s) 938
RsaI GTAC 3 cut(s) 323, 828, 929
RsaNI GTAC 3 cut(s) 322, 827, 928
RseI CAYNNNNRTG 3 cut(s) 86, 374, 881
SacI GAGCTC 1 cut(s) 449
SaqAI TTAA 2 cut(s) 390, 1053
SatI GCNGC 8 cut(s) 330, 348, 460, 463, 523, 563, 760, 848
Sau3AI GATC 3 cut(s) 393, 690, 1035
Sau96I GGNCC 5 cut(s) 58, 599, 637, 645, 761
SchI GAGTC 3 cut(s) 833, 914, 929
ScrFI CCNGG 2 cut(s) 431, 488
SduI GDGCHC 2 cut(s) 449, 822
SfaNI GCATC 2 cut(s) 658, 687
SfoI GGCGCC 1 cut(s) 165
Sfr274I CTCGAG 1 cut(s) 841
SfuI TTCGAA 1 cut(s) 834
SinI GGWCC 2 cut(s) 58, 637
SlaI CTCGAG 1 cut(s) 841
SmiMI CAYNNNNRTG 3 cut(s) 86, 374, 881
SmlI CTYRAG 5 cut(s) 41, 53, 594, 719, 841
SmoI CTYRAG 5 cut(s) 41, 53, 594, 719, 841
Sse9I AATT 3 cut(s) 655, 741, 830
SsiI CCGC 9 cut(s) 176, 327, 459, 462, 562, 665, 759, 869, 994
SspDI GGCGCC 1 cut(s) 163
SspI AATATT 2 cut(s) 22, 1057
SspMI CTAG 2 cut(s) 606, 966
SstI GAGCTC 1 cut(s) 449
StyD4I CCNGG 2 cut(s) 429, 486
StyI CCWWGG 2 cut(s) 641, 1071
TaaI ACNGT 6 cut(s) 49, 183, 198, 266, 321, 956
TaiI ACGT 3 cut(s) 426, 933, 1031
TaqI TCGA 4 cut(s) 745, 834, 842, 1038
TasI AATT 3 cut(s) 655, 741, 830
TatI WGTACW 1 cut(s) 321
TauI GCSGC 4 cut(s) 462, 465, 565, 762
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 2 cut(s) 390, 1053
Tru9I TTAA 2 cut(s) 390, 1053
TscAI CASTG 2 cut(s) 403, 961
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 4 cut(s) 329, 347, 522, 847
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 3 cut(s) 149, 546, 703
TspGWI ACGGA 1 cut(s) 928
TspRI CASTG 2 cut(s) 403, 961
Tth111I GACNNNGTC 1 cut(s) 938
VpaK11BI GGWCC 2 cut(s) 58, 637
XceI RCATGY 1 cut(s) 373
XhoI CTCGAG 1 cut(s) 841
XspI CTAG 2 cut(s) 606, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.