MD02G1150900.v1.1

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012891
Physical Location & Seq
Reverse (-)
12436359 .. 12438713
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1150900.v1.1.491

Sequence Viewer

Length: 1119 bp
ATGAGGAAGCTTTGTCCAAATATTGATAAAGAAGATGGCCTCGAGACGGTTCTTGAGGTCCCCATACCAGAAGAGATGCTCACAAGTATGGGAAGCAATGTGCAATTGCGATGGCAAAACATGTTAACGTGGATGAAGGCTCAAACTCCTGACAAATGGTCAGCGCCGGTCATCGCCGGACGCTTAAACGAGCTGCGCTTTTTGCTTTACCTTGTTGGCTCTCCTCTCATGCCTCTTCAAGTTCAATTGGGACATTCTGTACATCGTCCAGTTAGAGATTCTTCCATTCAAGCTTCAACAGCCAAATACATCGTGCAACAGTACACAGCCGCAACGGGAGGGCAACCAGCACTGAACTCGGTGACGAGCATGTGTGTGACCGGCGAGGTTAAGATCAGTGCATCGGATTTTCACCAAGGGGACGTGAGCGTTGGGGTCAAAAGGAGCTCAGACGAGAGGGGTGGTTTCGTGCTGTGGCAAAAGAATCCGGACTTGTGGTGCTTAGAGCTTGTTGTGTCTGGCTGCAAGGTCATCTGTGGAAGCAATGGGAAGCTTTCATGGAGGCATTCCTCTAACCAACAAGCACCCGTCTCTCAAGGCCCTCCTAGACCCTTGCGTCGTTTTCTGCAGGGTTTGGATCCAAGGGCCACGGCCAATCTATTCGCAGACGCAATGTGCATTGGTGAGAAGATCATCCACGACGATGATTGCTTCATTCTGAAGCTTGAAACAAGTCCTGCTATTCGTGAGGCACAGAGTGGTCCAAATTATGAGATAATTCACCACACAATATGGGGATATTTTAGCCAGCGATCGGGTCTACTGGTGAAATTTGAGGACTCGAGGCTGCTTAGCATGAAGAAAAAAGGGGACGACACGGATGTGTTTTGGGAGACAAGCACCGAGTCGGTGATACATGATTACAAGTACGTCGACGGCGTCAACATTGCTCACAGTGGGAGGACTAGGGTTAAGGTGTTCAGATATGGTGAGCAGTCTGCAAACCACAAGAGGGAGATGGAGGAAACGTGGCAGATTGATGAGGTGGATTTTAATATATGGGGTTTGACAATTGAGAGCTTTCTGCCCCCTGCTGAAACGAAGCAAGGATATAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

373

Amino Acids

41.83

Weight (kDa)

6.22

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 124 - 364 5.1e-115 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012891)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66740
fragaria_vesca FvH4_1g13560
malus_domestica MD02G1150900.v1.1 MD15G1265400.v1.1
prunus_persica Prupe.7G152200_v2.0.a1
pyrus_communis pycom02g11850 pycom15g23110
rosa_chinensis RchiOBHm_Chr2g0101971
rosa_laevigata RLG00000017120
rosa_multiflora Rmu_co8388187.1_g000001
rosa_roxburghii Rroxscaffold_2G00140880
rosa_rugosa Rorug02G0103400 Rorug02G0103500
rosa_samantha Rh2AG151600 Rh2BG157000 Rh2CG157000 Rh2DG157100
rosa_wichuraiana Rw2G011830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 615
AccI GTMKAC 2 cut(s) 820, 933
AccIII TCCGGA 1 cut(s) 487
AciI CCGC 1 cut(s) 330
AclWI GGATC 2 cut(s) 632, 645
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 1 cut(s) 830
AcuI CTGAAG 1 cut(s) 740
AcyI GRCGYC 1 cut(s) 939
AdeI CACNNNGTG 1 cut(s) 758
AfaI GTAC 3 cut(s) 261, 323, 929
AfiI CCNNNNNNNGG 3 cut(s) 46, 814, 1012
AflIII ACRYGT 1 cut(s) 120
AgsI TTSAA 5 cut(s) 239, 245, 290, 297, 728
AhdI GACNNNNNGTC 1 cut(s) 157
AjiI CACGTC 1 cut(s) 424
AleI CACNNNNGTG 1 cut(s) 881
AluBI AGCT 8 cut(s) 10, 193, 293, 447, 508, 553, 724, 1080
AluI AGCT 8 cut(s) 10, 193, 293, 447, 508, 553, 724, 1080
Alw21I GWGCWC 1 cut(s) 449
Alw26I GTCTC 3 cut(s) 38, 595, 887
AlwI GGATC 2 cut(s) 632, 645
Ama87I CYCGRG 2 cut(s) 41, 841
Aor13HI TCCGGA 1 cut(s) 487
AoxI GGCC 4 cut(s) 37, 598, 645, 651
ApeKI GCWGC 3 cut(s) 193, 522, 847
ApoI RAATTY 1 cut(s) 830
AspLEI GCGC 2 cut(s) 166, 198
AspS9I GGNCC 4 cut(s) 58, 599, 645, 761
AsuHPI GGTGA 7 cut(s) 373, 404, 695, 773, 838, 922, 1001
AvaI CYCGRG 2 cut(s) 41, 841
AvaII GGWCC 2 cut(s) 58, 761
BamHI GGATCC 1 cut(s) 637
BanII GRGCYC 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 449
BbvI GCAGC 3 cut(s) 180, 509, 834
BccI CCATC 3 cut(s) 29, 105, 1012
BceAI ACGGC 2 cut(s) 666, 952
BcgI CGANNNNNNTGC 2 cut(s) 339, 373
BcoDI GTCTC 3 cut(s) 38, 595, 887
BfaI CTAG 2 cut(s) 606, 966
BfmI CTRYAG 1 cut(s) 626
BfoI RGCGCY 1 cut(s) 167
BisI GCNGC 4 cut(s) 194, 330, 523, 848
BlpI GCTNAGC 1 cut(s) 851
BlsI GCNGC 4 cut(s) 195, 331, 524, 849
Bme18I GGWCC 2 cut(s) 58, 761
BmeRI GACNNNNNGTC 1 cut(s) 157
BmeT110I CYCGRG 2 cut(s) 41, 841
BmgBI CACGTC 1 cut(s) 424
BmgT120I GGNCC 4 cut(s) 58, 599, 645, 761
BmiI GGNNCC 2 cut(s) 60, 639
BmsI GCATC 2 cut(s) 66, 410
Bpu1102I GCTNAGC 1 cut(s) 851
BpuEI CTTGAG 2 cut(s) 74, 579
BsaHI GRCGYC 1 cut(s) 939
BsaJI CCNNGG 3 cut(s) 415, 641, 648
BsaWI WCCGGW 1 cut(s) 487
BsaXI ACNNNNNCTCC 2 cut(s) 1013, 1043
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 814, 1012
Bse118I RCCGGY 2 cut(s) 166, 380
Bse1I ACTGG 2 cut(s) 269, 828
Bse3DI GCAATG 4 cut(s) 103, 550, 678, 945
BseAI TCCGGA 1 cut(s) 487
BseDI CCNNGG 3 cut(s) 415, 641, 648
BseGI GGATG 3 cut(s) 138, 693, 886
BseLI CCNNNNNNNGG 3 cut(s) 46, 814, 1012
BseMI GCAATG 4 cut(s) 103, 550, 678, 945
BseMII CTCAG 1 cut(s) 462
BseNI ACTGG 2 cut(s) 269, 828
BseRI GAGGAG 1 cut(s) 213
BseXI GCAGC 3 cut(s) 180, 509, 834
Bsh1285I CGRYCG 1 cut(s) 815
BshFI GGCC 4 cut(s) 39, 600, 647, 653
BsiEI CGRYCG 1 cut(s) 815
BsiHKAI GWGCWC 1 cut(s) 449
BsiHKCI CYCGRG 2 cut(s) 41, 841
BsiSI CCGG 4 cut(s) 167, 177, 381, 488
BslFI GGGAC 4 cut(s) 44, 264, 434, 884
BslI CCNNNNNNNGG 3 cut(s) 46, 814, 1012
BsmAI GTCTC 3 cut(s) 38, 595, 887
BsmBI CGTCTC 2 cut(s) 38, 595
BsmFI GGGAC 4 cut(s) 44, 264, 434, 884
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 4 cut(s) 39, 600, 647, 653
BsoBI CYCGRG 2 cut(s) 41, 841
Bsp1286I GDGCHC 1 cut(s) 449
Bsp13I TCCGGA 1 cut(s) 487
Bsp1407I TGTACA 1 cut(s) 259
Bsp143I GATC 4 cut(s) 393, 637, 690, 812
Bsp1720I GCTNAGC 1 cut(s) 851
BspACI CCGC 1 cut(s) 330
BspANI GGCC 4 cut(s) 39, 600, 647, 653
BspCNI CTCAG 1 cut(s) 461
BspEI TCCGGA 1 cut(s) 487
BspLI GGNNCC 2 cut(s) 60, 639
BspMAI CTGCAG 1 cut(s) 630
BspPI GGATC 2 cut(s) 632, 645
BsrDI GCAATG 4 cut(s) 103, 550, 678, 945
BsrFI RCCGGY 2 cut(s) 166, 380
BsrGI TGTACA 1 cut(s) 259
BsrI ACTGG 2 cut(s) 269, 828
BssAI RCCGGY 2 cut(s) 166, 380
BssECI CCNNGG 3 cut(s) 415, 641, 648
BssMI GATC 4 cut(s) 393, 637, 690, 812
BssNI GRCGYC 1 cut(s) 939
BssT1I CCWWGG 2 cut(s) 415, 641
Bst4CI ACNGT 3 cut(s) 49, 321, 956
Bst6I CTCTTC 2 cut(s) 66, 240
BstACI GRCGYC 1 cut(s) 939
BstAUI TGTACA 1 cut(s) 259
BstC8I GCNNGC 1 cut(s) 809
BstDEI CTNAG 3 cut(s) 448, 502, 851
BstDSI CCRYGG 1 cut(s) 648
BstF5I GGATG 3 cut(s) 138, 693, 886
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 2 cut(s) 166, 198
BstKTI GATC 4 cut(s) 396, 640, 693, 815
BstMAI GTCTC 3 cut(s) 38, 595, 887
BstMBI GATC 4 cut(s) 393, 637, 690, 812
BstMCI CGRYCG 1 cut(s) 815
BstMWI GCNNNNNNNGC 2 cut(s) 202, 299
BstNSI RCATGY 2 cut(s) 124, 373
BstSFI CTRYAG 1 cut(s) 626
BstV1I GCAGC 3 cut(s) 180, 509, 834
BstX2I RGATCY 1 cut(s) 637
BstYI RGATCY 1 cut(s) 637
BsuRI GGCC 4 cut(s) 39, 600, 647, 653
BtgI CCRYGG 1 cut(s) 648
BtgZI GCGATG 2 cut(s) 124, 157
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 3 cut(s) 138, 693, 886
BtsIMutI CAGTG 3 cut(s) 350, 403, 961
Cac8I GCNNGC 1 cut(s) 809
CfoI GCGC 2 cut(s) 166, 198
Cfr10I RCCGGY 2 cut(s) 166, 380
Cfr13I GGNCC 4 cut(s) 58, 599, 645, 761
CseI GACGC 4 cut(s) 189, 605, 677, 928
Csp6I GTAC 3 cut(s) 260, 322, 928
CviAII CATG 6 cut(s) 121, 229, 370, 558, 856, 917
CviQI GTAC 3 cut(s) 260, 322, 928
DdeI CTNAG 3 cut(s) 448, 502, 851
DpnI GATC 4 cut(s) 395, 639, 692, 814
DpnII GATC 4 cut(s) 393, 637, 690, 812
DraIII CACNNNGTG 1 cut(s) 758
DrdI GACNNNNNNGTC 1 cut(s) 615
DriI GACNNNNNGTC 1 cut(s) 157
DseDI GACNNNNNNGTC 1 cut(s) 615
EaeI YGGCCR 1 cut(s) 651
Eam1104I CTCTTC 2 cut(s) 66, 240
Eam1105I GACNNNNNGTC 1 cut(s) 157
EarI CTCTTC 2 cut(s) 66, 240
Ecl136II GAGCTC 1 cut(s) 447
Eco130I CCWWGG 2 cut(s) 415, 641
Eco24I GRGCYC 1 cut(s) 449
Eco47I GGWCC 2 cut(s) 58, 761
Eco53kI GAGCTC 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 740
Eco88I CYCGRG 2 cut(s) 41, 841
EcoICRI GAGCTC 1 cut(s) 447
EcoO109I RGGNCCY 2 cut(s) 58, 599
EcoT14I CCWWGG 2 cut(s) 415, 641
EcoT38I GRGCYC 1 cut(s) 449
ErhI CCWWGG 2 cut(s) 415, 641
Esp3I CGTCTC 2 cut(s) 38, 595
FaeI CATG 6 cut(s) 124, 232, 373, 561, 859, 920
FaqI GGGAC 4 cut(s) 44, 264, 434, 884
FatI CATG 6 cut(s) 120, 228, 369, 557, 855, 916
FblI GTMKAC 2 cut(s) 820, 933
Fnu4HI GCNGC 4 cut(s) 194, 330, 523, 848
FokI GGATG 3 cut(s) 145, 680, 893
FriOI GRGCYC 1 cut(s) 449
Fsp4HI GCNGC 4 cut(s) 194, 330, 523, 848
FspBI CTAG 2 cut(s) 606, 966
GlaI GCGC 2 cut(s) 165, 197
GluI GCNGC 4 cut(s) 194, 330, 523, 848
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 4 cut(s) 39, 600, 647, 653
HapII CCGG 4 cut(s) 167, 177, 381, 488
HgaI GACGC 4 cut(s) 189, 605, 677, 928
HhaI GCGC 2 cut(s) 166, 198
Hin1I GRCGYC 1 cut(s) 939
Hin1II CATG 6 cut(s) 124, 232, 373, 561, 859, 920
Hin6I GCGC 2 cut(s) 164, 196
HinP1I GCGC 2 cut(s) 164, 196
HincII GTYRAC 3 cut(s) 126, 934, 943
HindII GTYRAC 3 cut(s) 126, 934, 943
HindIII AAGCTT 4 cut(s) 8, 291, 551, 722
HinfI GANTC 4 cut(s) 278, 484, 839, 905
HpaI GTTAAC 1 cut(s) 126
HpaII CCGG 4 cut(s) 167, 177, 381, 488
HphI GGTGA 7 cut(s) 373, 404, 695, 773, 838, 922, 1001
Hpy166II GTNNAC 5 cut(s) 126, 324, 821, 934, 943
Hpy188I TCNGA 4 cut(s) 406, 451, 720, 983
Hpy188III TCNNGA 5 cut(s) 43, 53, 149, 488, 746
Hpy8I GTNNAC 5 cut(s) 126, 324, 821, 934, 943
Hpy99I CGWCG 4 cut(s) 621, 704, 935, 938
HpyAV CCTTC 1 cut(s) 130
HpyCH4III ACNGT 3 cut(s) 49, 321, 956
HpyCH4IV ACGT 4 cut(s) 128, 423, 930, 1028
HpyCH4V TGCA 7 cut(s) 103, 316, 401, 525, 628, 678, 1001
HpyF10VI GCNNNNNNNGC 2 cut(s) 202, 299
HpyF3I CTNAG 3 cut(s) 448, 502, 851
HpySE526I ACGT 4 cut(s) 128, 423, 930, 1028
Hsp92I GRCGYC 1 cut(s) 939
Hsp92II CATG 6 cut(s) 124, 232, 373, 561, 859, 920
HspAI GCGC 2 cut(s) 164, 196
Kpn2I TCCGGA 1 cut(s) 487
KspAI GTTAAC 1 cut(s) 126
Kzo9I GATC 4 cut(s) 393, 637, 690, 812
LmnI GCTCC 1 cut(s) 444
Lsp1109I GCAGC 3 cut(s) 180, 509, 834
LweI GCATC 2 cut(s) 66, 410
MaeI CTAG 2 cut(s) 606, 966
MaeII ACGT 4 cut(s) 128, 423, 930, 1028
MaeIII GTNAC 2 cut(s) 361, 376
MalI GATC 4 cut(s) 395, 639, 692, 814
MboI GATC 4 cut(s) 393, 637, 690, 812
MboII GAAGA 6 cut(s) 44, 83, 227, 273, 700, 871
MfeI CAATTG 3 cut(s) 104, 245, 1071
MflI RGATCY 1 cut(s) 637
MhlI GDGCHC 1 cut(s) 449
MluCI AATT 6 cut(s) 104, 245, 766, 777, 830, 1071
MlyI GAGTC 2 cut(s) 833, 914
MroI TCCGGA 1 cut(s) 487
MseI TTAA 5 cut(s) 125, 185, 390, 972, 1053
MslI CAYNNNNRTG 4 cut(s) 86, 374, 702, 881
MspI CCGG 4 cut(s) 167, 177, 381, 488
MunI CAATTG 3 cut(s) 104, 245, 1071
Mva1269I GAATGC 1 cut(s) 565
MwoI GCNNNNNNNGC 2 cut(s) 202, 299
NdeII GATC 4 cut(s) 393, 637, 690, 812
NlaIII CATG 6 cut(s) 124, 232, 373, 561, 859, 920
NlaIV GGNNCC 2 cut(s) 60, 639
NmuCI GTSAC 2 cut(s) 361, 376
NspI RCATGY 2 cut(s) 124, 373
OliI CACNNNNGTG 1 cut(s) 881
PaeR7I CTCGAG 2 cut(s) 41, 841
PciI ACATGT 1 cut(s) 120
PcsI WCGNNNNNNNCGW 1 cut(s) 936
PctI GAATGC 1 cut(s) 565
PfeI GAWTC 2 cut(s) 278, 484
PflFI GACNNNGTC 1 cut(s) 938
PkrI GCNGC 4 cut(s) 195, 331, 524, 849
Ple19I CGATCG 1 cut(s) 815
PleI GAGTC 2 cut(s) 833, 913
PpsI GAGTC 2 cut(s) 833, 913
PpuMI RGGWCCY 1 cut(s) 58
PscI ACATGT 1 cut(s) 120
Psp124BI GAGCTC 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 58
PspN4I GGNNCC 2 cut(s) 60, 639
PspPI GGNCC 4 cut(s) 58, 599, 645, 761
PspPPI RGGWCCY 1 cut(s) 58
PspXI VCTCGAGB 1 cut(s) 841
PstI CTGCAG 1 cut(s) 630
PsuI RGATCY 1 cut(s) 637
PsyI GACNNNGTC 1 cut(s) 938
PvuI CGATCG 1 cut(s) 815
RsaI GTAC 3 cut(s) 261, 323, 929
RsaNI GTAC 3 cut(s) 260, 322, 928
RseI CAYNNNNRTG 4 cut(s) 86, 374, 702, 881
SacI GAGCTC 1 cut(s) 449
SalI GTCGAC 1 cut(s) 932
SaqAI TTAA 5 cut(s) 125, 185, 390, 972, 1053
SatI GCNGC 4 cut(s) 194, 330, 523, 848
Sau3AI GATC 4 cut(s) 393, 637, 690, 812
Sau96I GGNCC 4 cut(s) 58, 599, 645, 761
SchI GAGTC 2 cut(s) 833, 914
SduI GDGCHC 1 cut(s) 449
SfaNI GCATC 2 cut(s) 66, 410
SfcI CTRYAG 1 cut(s) 626
Sfr274I CTCGAG 2 cut(s) 41, 841
SgrDI CGTCGACG 1 cut(s) 932
SinI GGWCC 2 cut(s) 58, 761
SlaI CTCGAG 2 cut(s) 41, 841
SmiMI CAYNNNNRTG 4 cut(s) 86, 374, 702, 881
SmlI CTYRAG 4 cut(s) 41, 53, 594, 841
SmoI CTYRAG 4 cut(s) 41, 53, 594, 841
Sse9I AATT 6 cut(s) 104, 245, 766, 777, 830, 1071
SsiI CCGC 1 cut(s) 330
SspI AATATT 1 cut(s) 22
SspMI CTAG 2 cut(s) 606, 966
SstI GAGCTC 1 cut(s) 449
StyI CCWWGG 2 cut(s) 415, 641
TaaI ACNGT 3 cut(s) 49, 321, 956
TaiI ACGT 4 cut(s) 131, 426, 933, 1031
TaqI TCGA 3 cut(s) 42, 842, 933
TasI AATT 6 cut(s) 104, 245, 766, 777, 830, 1071
TatI WGTACW 2 cut(s) 259, 321
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 2 cut(s) 278, 484
Tru1I TTAA 5 cut(s) 125, 185, 390, 972, 1053
Tru9I TTAA 5 cut(s) 125, 185, 390, 972, 1053
TscAI CASTG 3 cut(s) 357, 403, 961
TseFI GTSAC 2 cut(s) 361, 376
TseI GCWGC 3 cut(s) 193, 522, 847
Tsp45I GTSAC 2 cut(s) 361, 376
TspDTI ATGAA 4 cut(s) 149, 546, 703, 872
TspGWI ACGGA 1 cut(s) 893
TspRI CASTG 3 cut(s) 357, 403, 961
Tth111I GACNNNGTC 1 cut(s) 938
VpaK11BI GGWCC 2 cut(s) 58, 761
XapI RAATTY 1 cut(s) 830
XceI RCATGY 2 cut(s) 124, 373
XhoI CTCGAG 2 cut(s) 41, 841
XmiI GTMKAC 2 cut(s) 820, 933
XspI CTAG 2 cut(s) 606, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.