pycom02g11850

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012891
Physical Location & Seq
Reverse (-)
8414123 .. 8414929
807 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g11850.1

Sequence Viewer

Length: 459 bp
ATGAGATCGATAATACACATGAGGAAGCTTTGTCCAAATATTGATAAAGAAGATGGCCTCGAGACCGTTCTTGAGGTCCCCATACCAGAAGAGATGTTCACGAGTATGGGAAGCAATGTGCAATTGCGATGGCAAAACATGTTAACGTGGATGAAGGCTCAAACTTCTGACAAATGGTCTGCGCCGGTCATCGCCGGACGCTTAAACGAGCTGCGCTTTTTGCTTTACCTTGTTGGTTCTCCTCTCATCCCTCTTCAAGTTCAATTGGGACATTCTGTACATCGTCCAGTTAGAGATTCTTCCATTCAAGCTTCAACAGCAAAATACATCGTGCAACAGTACACAGCCGCAACGGGAGGGCAACCAGCACTGAACTCGGTGAAGAGCATGTGTGTGACCGGCGAGGTTAAGATCAGTGCATCGGATTTTCACCAAGGGGACGTGAGCGTTGAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

153

Amino Acids

16.84

Weight (kDa)

6.9

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012891)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66740
fragaria_vesca FvH4_1g13560
malus_domestica MD02G1150900.v1.1 MD15G1265400.v1.1
prunus_persica Prupe.7G152200_v2.0.a1
pyrus_communis pycom02g11850 pycom15g23110
rosa_chinensis RchiOBHm_Chr2g0101971
rosa_laevigata RLG00000017120
rosa_multiflora Rmu_co8388187.1_g000001
rosa_roxburghii Rroxscaffold_2G00140880
rosa_rugosa Rorug02G0103400 Rorug02G0103500
rosa_samantha Rh2AG151600 Rh2BG157000 Rh2CG157000 Rh2DG157100
rosa_wichuraiana Rw2G011830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 348
AfaI GTAC 2 cut(s) 279, 341
AflIII ACRYGT 1 cut(s) 138
AgsI TTSAA 4 cut(s) 257, 263, 308, 315
AhdI GACNNNNNGTC 1 cut(s) 175
AjiI CACGTC 1 cut(s) 442
AluBI AGCT 3 cut(s) 28, 211, 311
AluI AGCT 3 cut(s) 28, 211, 311
Alw26I GTCTC 1 cut(s) 56
Ama87I CYCGRG 1 cut(s) 59
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 211
AspLEI GCGC 2 cut(s) 184, 216
AspS9I GGNCC 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 391, 422
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 1 cut(s) 76
BauI CACGAG 1 cut(s) 100
BbvI GCAGC 1 cut(s) 198
BccI CCATC 2 cut(s) 47, 123
BcgI CGANNNNNNTGC 2 cut(s) 357, 391
BcoDI GTCTC 1 cut(s) 56
BisI GCNGC 2 cut(s) 212, 348
BlsI GCNGC 2 cut(s) 213, 349
Bme18I GGWCC 1 cut(s) 76
BmeRI GACNNNNNGTC 1 cut(s) 175
BmeT110I CYCGRG 1 cut(s) 59
BmgBI CACGTC 1 cut(s) 442
BmgT120I GGNCC 1 cut(s) 76
BmiI GGNNCC 1 cut(s) 78
BmsI GCATC 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 92
Bsa29I ATCGAT 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 1 cut(s) 433
Bse118I RCCGGY 2 cut(s) 184, 398
Bse1I ACTGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 121
BseCI ATCGAT 1 cut(s) 8
BseDI CCNNGG 1 cut(s) 433
BseGI GGATG 2 cut(s) 156, 246
BseMI GCAATG 1 cut(s) 121
BseNI ACTGG 1 cut(s) 287
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 198
BshFI GGCC 1 cut(s) 57
BshVI ATCGAT 1 cut(s) 8
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 3 cut(s) 185, 195, 399
BslFI GGGAC 3 cut(s) 62, 282, 452
BsmAI GTCTC 1 cut(s) 56
BsmFI GGGAC 3 cut(s) 62, 282, 452
BsnI GGCC 1 cut(s) 57
Bso31I GGTCTC 1 cut(s) 56
BsoBI CYCGRG 1 cut(s) 59
Bsp1407I TGTACA 1 cut(s) 277
Bsp143I GATC 2 cut(s) 5, 411
BspACI CCGC 1 cut(s) 348
BspANI GGCC 1 cut(s) 57
BspDI ATCGAT 1 cut(s) 8
BspLI GGNNCC 1 cut(s) 78
BspQI GCTCTTC 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 121
BsrFI RCCGGY 2 cut(s) 184, 398
BsrGI TGTACA 1 cut(s) 277
BsrI ACTGG 1 cut(s) 287
BssAI RCCGGY 2 cut(s) 184, 398
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 2 cut(s) 5, 411
BssSI CACGAG 1 cut(s) 100
BssT1I CCWWGG 1 cut(s) 433
Bst2BI CACGAG 1 cut(s) 100
Bst4CI ACNGT 2 cut(s) 67, 339
Bst6I CTCTTC 3 cut(s) 84, 258, 377
BstAUI TGTACA 1 cut(s) 277
BstF5I GGATG 2 cut(s) 156, 246
BstHHI GCGC 2 cut(s) 184, 216
BstKTI GATC 2 cut(s) 8, 414
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 2 cut(s) 5, 411
BstMWI GCNNNNNNNGC 2 cut(s) 220, 317
BstNSI RCATGY 2 cut(s) 142, 391
BstV1I GCAGC 1 cut(s) 198
Bsu15I ATCGAT 1 cut(s) 8
BsuRI GGCC 1 cut(s) 57
BsuTUI ATCGAT 1 cut(s) 8
BtgZI GCGATG 2 cut(s) 142, 175
BtrI CACGTC 1 cut(s) 442
BtsCI GGATG 2 cut(s) 156, 246
BtsIMutI CAGTG 2 cut(s) 368, 421
CfoI GCGC 2 cut(s) 184, 216
Cfr10I RCCGGY 2 cut(s) 184, 398
Cfr13I GGNCC 1 cut(s) 76
ClaI ATCGAT 1 cut(s) 8
CseI GACGC 1 cut(s) 207
Csp6I GTAC 2 cut(s) 278, 340
CviAII CATG 3 cut(s) 19, 139, 388
CviJI RGCY 6 cut(s) 28, 57, 158, 211, 311, 347
CviKI_1 RGCY 6 cut(s) 28, 57, 158, 211, 311, 347
CviQI GTAC 2 cut(s) 278, 340
DpnI GATC 2 cut(s) 7, 413
DpnII GATC 2 cut(s) 5, 411
DriI GACNNNNNGTC 1 cut(s) 175
Eam1104I CTCTTC 3 cut(s) 84, 258, 377
Eam1105I GACNNNNNGTC 1 cut(s) 175
EarI CTCTTC 3 cut(s) 84, 258, 377
Eco130I CCWWGG 1 cut(s) 433
Eco31I GGTCTC 1 cut(s) 56
Eco47I GGWCC 1 cut(s) 76
Eco88I CYCGRG 1 cut(s) 59
EcoO109I RGGNCCY 1 cut(s) 76
EcoT14I CCWWGG 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 433
FaeI CATG 3 cut(s) 22, 142, 391
FaiI YATR 5 cut(s) 20, 83, 107, 140, 389
FaqI GGGAC 3 cut(s) 62, 282, 452
FatI CATG 3 cut(s) 18, 138, 387
Fnu4HI GCNGC 2 cut(s) 212, 348
FokI GGATG 2 cut(s) 163, 233
Fsp4HI GCNGC 2 cut(s) 212, 348
GlaI GCGC 2 cut(s) 183, 215
GluI GCNGC 2 cut(s) 212, 348
HaeIII GGCC 1 cut(s) 57
HapII CCGG 3 cut(s) 185, 195, 399
HgaI GACGC 1 cut(s) 207
HhaI GCGC 2 cut(s) 184, 216
Hin1II CATG 3 cut(s) 22, 142, 391
Hin6I GCGC 2 cut(s) 182, 214
HinP1I GCGC 2 cut(s) 182, 214
HincII GTYRAC 1 cut(s) 144
HindII GTYRAC 1 cut(s) 144
HindIII AAGCTT 2 cut(s) 26, 309
HinfI GANTC 1 cut(s) 296
HpaI GTTAAC 1 cut(s) 144
HpaII CCGG 3 cut(s) 185, 195, 399
HphI GGTGA 2 cut(s) 391, 422
Hpy166II GTNNAC 3 cut(s) 99, 144, 342
Hpy188I TCNGA 2 cut(s) 169, 424
Hpy188III TCNNGA 3 cut(s) 61, 71, 100
Hpy8I GTNNAC 3 cut(s) 99, 144, 342
HpyAV CCTTC 1 cut(s) 148
HpyCH4III ACNGT 2 cut(s) 67, 339
HpyCH4IV ACGT 2 cut(s) 146, 441
HpyCH4V TGCA 3 cut(s) 121, 334, 419
HpyF10VI GCNNNNNNNGC 2 cut(s) 220, 317
HpySE526I ACGT 2 cut(s) 146, 441
Hsp92II CATG 3 cut(s) 22, 142, 391
HspAI GCGC 2 cut(s) 182, 214
KspAI GTTAAC 1 cut(s) 144
Kzo9I GATC 2 cut(s) 5, 411
LguI GCTCTTC 1 cut(s) 377
LpnPI CCDG 6 cut(s) 99, 198, 208, 300, 378, 412
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 1 cut(s) 428
MaeII ACGT 2 cut(s) 146, 441
MaeIII GTNAC 1 cut(s) 394
MalI GATC 2 cut(s) 7, 413
MboI GATC 2 cut(s) 5, 411
MboII GAAGA 5 cut(s) 62, 101, 245, 291, 394
MfeI CAATTG 2 cut(s) 122, 263
MluCI AATT 2 cut(s) 122, 263
MnlI CCTC 8 cut(s) 15, 67, 68, 252, 261, 350, 397, 445
MseI TTAA 3 cut(s) 143, 203, 408
MslI CAYNNNNRTG 2 cut(s) 104, 392
MspI CCGG 3 cut(s) 185, 195, 399
MunI CAATTG 2 cut(s) 122, 263
MwoI GCNNNNNNNGC 2 cut(s) 220, 317
NdeII GATC 2 cut(s) 5, 411
NlaIII CATG 3 cut(s) 22, 142, 391
NlaIV GGNNCC 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 394
NspI RCATGY 2 cut(s) 142, 391
PaeR7I CTCGAG 1 cut(s) 59
PciI ACATGT 1 cut(s) 138
PciSI GCTCTTC 1 cut(s) 377
PfeI GAWTC 1 cut(s) 296
PkrI GCNGC 2 cut(s) 213, 349
PpuMI RGGWCCY 1 cut(s) 76
PscI ACATGT 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 76
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 1 cut(s) 76
PspPPI RGGWCCY 1 cut(s) 76
RsaI GTAC 2 cut(s) 279, 341
RsaNI GTAC 2 cut(s) 278, 340
RseI CAYNNNNRTG 2 cut(s) 104, 392
SapI GCTCTTC 1 cut(s) 377
SaqAI TTAA 3 cut(s) 143, 203, 408
SatI GCNGC 2 cut(s) 212, 348
Sau3AI GATC 2 cut(s) 5, 411
Sau96I GGNCC 1 cut(s) 76
SetI ASST 8 cut(s) 30, 78, 149, 213, 231, 313, 408, 444
SfaNI GCATC 1 cut(s) 428
Sfr274I CTCGAG 1 cut(s) 59
SinI GGWCC 1 cut(s) 76
SlaI CTCGAG 1 cut(s) 59
SmiMI CAYNNNNRTG 2 cut(s) 104, 392
SmlI CTYRAG 2 cut(s) 59, 71
SmoI CTYRAG 2 cut(s) 59, 71
Sse9I AATT 2 cut(s) 122, 263
SsiI CCGC 1 cut(s) 348
SspI AATATT 1 cut(s) 40
StyI CCWWGG 1 cut(s) 433
TaaI ACNGT 2 cut(s) 67, 339
TaiI ACGT 2 cut(s) 149, 444
TaqI TCGA 2 cut(s) 8, 60
TasI AATT 2 cut(s) 122, 263
TatI WGTACW 2 cut(s) 277, 339
TauI GCSGC 1 cut(s) 350
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 3 cut(s) 143, 203, 408
Tru9I TTAA 3 cut(s) 143, 203, 408
TscAI CASTG 2 cut(s) 375, 421
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 1 cut(s) 211
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 1 cut(s) 167
TspRI CASTG 2 cut(s) 375, 421
VpaK11BI GGWCC 1 cut(s) 76
XceI RCATGY 2 cut(s) 142, 391
XhoI CTCGAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.