FvH4_1g13560

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012891
Physical Location & Seq
Forward (+)
7452006 .. 7454534
2529 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g13560.t1

Sequence Viewer

Length: 1125 bp
ATGAGGAAGCTTTGTCCGAATATTGATAGAGACGATGGCCTCGAGACTGTTCTCGAGGTCCCTATACCGGAAGAGATGTTCACTAGTATGGGAAGCAATGTCACCTTACGGTGGCAAAACATGCAGACATGGATGAAGGCTCAGACCTCCGACAAATGGTCGGCGCCGGTCATTGCCGGACGTTTTAATGAGCTCCGGTTCTTGCTTTACCTTGTAGGCTCTCCTCTTATCCCTCTTCAAGTTCAGTTGGGTCATACCGTTCACCGTCCAGTTAGAGATTCTTCAATTCAAGCTTCAACTGCCAAGTACATTGTGCAACAGTACACAGCGGCGACGGGAGGCCAAGCGGCATTGAACGCAGTAACCAGCATGTGTGTTACCGGGCAGGTTAAGATTAGTGCATCAGAATTTCACCAAGGGGACCTGAGCCTCGAGGTGAAAAAGAGCGCCGCCGAGAGTGGTGGATTTTTGCTTTGGCAAAAGAATCCAGACTTGTGGTGCTTAGAGCTTGTTGTGTCTGGCTGCAAGGTCATATGTGGAAGCAATGGCAAGCTTTCTTGGCGGCATTCCTCTAACCAACAAACTCCCATCTCACAAGGTCCTCCTAGACCCTTGCGTCGTTTTCTCCAGGGTTTGGACCCAAGAGCCACGGCACTTCTATTCGCAGATGCAATGTGCATCGGCGAGAAGATCATCAACAATGACGACTGCTTCATACTCAAGCTGGAAACAAGTCCGGCGATTCGCGAGCAACAAAGGGGTCCAAACTATGAGATAATCCACCACACAATATGGGGATACTTCAGCCAACGTTCTGGTCTCCTTGTACAATTCGAGGACTCAAGGCTACTTAGGATGACCAACAAGGGAGACGACACAGATGTGTTCTGGGAAACCAGCACTGAGTCTTTCATACAAGACTATAAGTACGTTGAAGGACTCAACATTGCGCACAGCGGAAAAACACGAGTCAAGGTTTTCAGATATGGCGAGCAGTCTGCAAACCACAAGAGGGAGATGGAAGAAACGTGGAAGATTCATGAGGTGGATTTTAACATATGGGGTTTGACTGCTGAGAGCTTTCTGCCTCCTGCTGATATAGATAAGAAGAAGCAGGACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

375

Amino Acids

42.27

Weight (kDa)

6.52

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 124 - 364 1.2e-112 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012891)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66740
fragaria_vesca FvH4_1g13560
malus_domestica MD02G1150900.v1.1 MD15G1265400.v1.1
prunus_persica Prupe.7G152200_v2.0.a1
pyrus_communis pycom02g11850 pycom15g23110
rosa_chinensis RchiOBHm_Chr2g0101971
rosa_laevigata RLG00000017120
rosa_multiflora Rmu_co8388187.1_g000001
rosa_roxburghii Rroxscaffold_2G00140880
rosa_rugosa Rorug02G0103400 Rorug02G0103500
rosa_samantha Rh2AG151600 Rh2BG157000 Rh2CG157000 Rh2DG157100
rosa_wichuraiana Rw2G011830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 615
AbsI CCTCGAGG 1 cut(s) 431
Acc16I TGCGCA 1 cut(s) 951
Acc36I ACCTGC 1 cut(s) 376
AccB1I GGYRCC 1 cut(s) 163
AccB7I CCANNNNNTGG 1 cut(s) 634
AccII CGCG 1 cut(s) 747
AciI CCGC 5 cut(s) 329, 347, 450, 562, 957
AclI AACGTT 1 cut(s) 811
AcsI RAATTY 1 cut(s) 407
AcuI CTGAAG 1 cut(s) 787
AcyI GRCGYC 1 cut(s) 164
AfaI GTAC 4 cut(s) 308, 323, 828, 929
AfiI CCNNNNNNNGG 5 cut(s) 67, 111, 156, 634, 1012
AgsI TTSAA 6 cut(s) 239, 285, 290, 297, 355, 935
AhdI GACNNNNNGTC 1 cut(s) 157
AhlI ACTAGT 1 cut(s) 83
AjnI CCWGG 1 cut(s) 627
AleI CACNNNNGTG 1 cut(s) 881
AloI GAACNNNNNNTCC 2 cut(s) 62, 94
AluBI AGCT 7 cut(s) 10, 193, 293, 508, 553, 724, 1080
AluI AGCT 7 cut(s) 10, 193, 293, 508, 553, 724, 1080
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 4 cut(s) 24, 38, 824, 864
Ama87I CYCGRG 3 cut(s) 41, 53, 431
AoxI GGCC 2 cut(s) 37, 340
ApeKI GCWGC 1 cut(s) 522
ApoI RAATTY 1 cut(s) 407
AspLEI GCGC 3 cut(s) 166, 449, 952
AspS9I GGNCC 5 cut(s) 58, 421, 599, 637, 761
AsuC2I CCSGG 1 cut(s) 382
AsuHPI GGTGA 4 cut(s) 94, 254, 404, 448
AvaI CYCGRG 3 cut(s) 41, 53, 431
AvaII GGWCC 5 cut(s) 58, 421, 599, 637, 761
BanI GGYRCC 1 cut(s) 163
BanII GRGCYC 1 cut(s) 195
BauI CACGAG 1 cut(s) 966
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 1 cut(s) 509
BccI CCATC 3 cut(s) 29, 596, 1012
BceAI ACGGC 1 cut(s) 666
BcgI CGANNNNNNTGC 2 cut(s) 980, 1014
BciT130I CCWGG 1 cut(s) 629
BciVI GTATCC 1 cut(s) 791
BcnI CCSGG 1 cut(s) 382
BcoDI GTCTC 4 cut(s) 24, 38, 824, 864
BcuI ACTAGT 1 cut(s) 83
BfaI CTAG 2 cut(s) 84, 606
BfoI RGCGCY 2 cut(s) 167, 450
BfuAI ACCTGC 1 cut(s) 376
BfuI GTATCC 1 cut(s) 791
BisI GCNGC 5 cut(s) 330, 348, 450, 523, 563
BlsI GCNGC 5 cut(s) 331, 349, 451, 524, 564
Bme1390I CCNGG 2 cut(s) 382, 629
Bme18I GGWCC 5 cut(s) 58, 421, 599, 637, 761
BmeRI GACNNNNNGTC 1 cut(s) 157
BmeT110I CYCGRG 3 cut(s) 41, 53, 431
BmgT120I GGNCC 5 cut(s) 58, 421, 599, 637, 761
BmiI GGNNCC 5 cut(s) 60, 165, 422, 639, 762
BmrFI CCNGG 2 cut(s) 382, 629
BmsI GCATC 3 cut(s) 410, 658, 687
BpmI CTGGAG 1 cut(s) 611
Bpu10I CCTNAGC 1 cut(s) 425
BpuEI CTTGAG 2 cut(s) 704, 826
BpuMI CCSGG 1 cut(s) 382
BsaHI GRCGYC 1 cut(s) 164
BsaI GGTCTC 1 cut(s) 824
BsaJI CCNNGG 3 cut(s) 415, 628, 648
BsaWI WCCGGW 2 cut(s) 67, 195
Bsc4I CCNNNNNNNGG 5 cut(s) 67, 111, 156, 634, 1012
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 1 cut(s) 269
Bse3DI GCAATG 5 cut(s) 103, 171, 550, 678, 945
BseBI CCWGG 1 cut(s) 629
BseDI CCNNGG 3 cut(s) 415, 628, 648
BseGI GGATG 2 cut(s) 138, 861
BseLI CCNNNNNNNGG 5 cut(s) 67, 111, 156, 634, 1012
BseMI GCAATG 5 cut(s) 103, 171, 550, 678, 945
BseMII CTCAG 4 cut(s) 155, 416, 894, 1065
BseNI ACTGG 1 cut(s) 269
BseRI GAGGAG 1 cut(s) 213
BseXI GCAGC 1 cut(s) 509
Bsh1236I CGCG 1 cut(s) 747
BshFI GGCC 2 cut(s) 39, 342
BshNI GGYRCC 1 cut(s) 163
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 3 cut(s) 41, 53, 431
BsiSI CCGG 6 cut(s) 68, 167, 177, 196, 381, 737
BslFI GGGAC 2 cut(s) 44, 434
BslI CCNNNNNNNGG 5 cut(s) 67, 111, 156, 634, 1012
BsmAI GTCTC 4 cut(s) 24, 38, 824, 864
BsmBI CGTCTC 2 cut(s) 24, 864
BsmFI GGGAC 2 cut(s) 44, 434
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 2 cut(s) 39, 342
Bso31I GGTCTC 1 cut(s) 824
BsoBI CYCGRG 3 cut(s) 41, 53, 431
Bsp1286I GDGCHC 1 cut(s) 195
Bsp1407I TGTACA 1 cut(s) 826
Bsp143I GATC 1 cut(s) 690
Bsp68I TCGCGA 1 cut(s) 747
BspACI CCGC 5 cut(s) 329, 347, 450, 562, 957
BspANI GGCC 2 cut(s) 39, 342
BspCNI CTCAG 4 cut(s) 154, 417, 895, 1066
BspFNI CGCG 1 cut(s) 747
BspHI TCATGA 1 cut(s) 1039
BspLI GGNNCC 5 cut(s) 60, 165, 422, 639, 762
BspMI ACCTGC 1 cut(s) 376
BspT107I GGYRCC 1 cut(s) 163
BspTNI GGTCTC 1 cut(s) 824
BsrDI GCAATG 5 cut(s) 103, 171, 550, 678, 945
BsrFI RCCGGY 1 cut(s) 166
BsrGI TGTACA 1 cut(s) 826
BsrI ACTGG 1 cut(s) 269
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 3 cut(s) 415, 628, 648
BssMI GATC 1 cut(s) 690
BssNI GRCGYC 1 cut(s) 164
BssSI CACGAG 1 cut(s) 966
BssT1I CCWWGG 1 cut(s) 415
Bst2BI CACGAG 1 cut(s) 966
Bst2UI CCWGG 1 cut(s) 629
Bst4CI ACNGT 6 cut(s) 49, 111, 259, 266, 321, 1121
Bst6I CTCTTC 2 cut(s) 66, 240
BstACI GRCGYC 1 cut(s) 164
BstAPI GCANNNNNTGC 1 cut(s) 121
BstAUI TGTACA 1 cut(s) 826
BstC8I GCNNGC 3 cut(s) 551, 749, 992
BstDEI CTNAG 6 cut(s) 141, 425, 502, 851, 903, 1074
BstDSI CCRYGG 1 cut(s) 648
BstF5I GGATG 2 cut(s) 138, 861
BstFNI CGCG 1 cut(s) 747
BstH2I RGCGCY 2 cut(s) 167, 450
BstHHI GCGC 3 cut(s) 166, 449, 952
BstKTI GATC 1 cut(s) 693
BstMAI GTCTC 4 cut(s) 24, 38, 824, 864
BstMBI GATC 1 cut(s) 690
BstMWI GCNNNNNNNGC 4 cut(s) 121, 299, 356, 559
BstNI CCWGG 1 cut(s) 629
BstNSI RCATGY 2 cut(s) 124, 373
BstSCI CCNGG 2 cut(s) 380, 627
BstUI CGCG 1 cut(s) 747
BstV1I GCAGC 1 cut(s) 509
BstXI CCANNNNNNTGG 2 cut(s) 495, 815
BsuI GTATCC 1 cut(s) 791
BsuRI GGCC 2 cut(s) 39, 342
BtgI CCRYGG 1 cut(s) 648
BtsCI GGATG 2 cut(s) 138, 861
BtsIMutI CAGTG 1 cut(s) 900
BtuMI TCGCGA 1 cut(s) 747
BveI ACCTGC 1 cut(s) 376
Cac8I GCNNGC 3 cut(s) 551, 749, 992
CciI TCATGA 1 cut(s) 1039
CfoI GCGC 3 cut(s) 166, 449, 952
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 5 cut(s) 58, 421, 599, 637, 761
CseI GACGC 1 cut(s) 605
Csp6I GTAC 4 cut(s) 307, 322, 827, 928
CviAII CATG 4 cut(s) 121, 129, 370, 1040
CviQI GTAC 4 cut(s) 307, 322, 827, 928
DdeI CTNAG 6 cut(s) 141, 425, 502, 851, 903, 1074
DinI GGCGCC 1 cut(s) 165
DpnI GATC 1 cut(s) 692
DpnII GATC 1 cut(s) 690
DrdI GACNNNNNNGTC 1 cut(s) 615
DriI GACNNNNNGTC 1 cut(s) 157
DseDI GACNNNNNNGTC 1 cut(s) 615
Eam1104I CTCTTC 2 cut(s) 66, 240
Eam1105I GACNNNNNGTC 1 cut(s) 157
EarI CTCTTC 2 cut(s) 66, 240
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 415
Eco24I GRGCYC 1 cut(s) 195
Eco31I GGTCTC 1 cut(s) 824
Eco47I GGWCC 5 cut(s) 58, 421, 599, 637, 761
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 787
Eco88I CYCGRG 3 cut(s) 41, 53, 431
EcoICRI GAGCTC 1 cut(s) 193
EcoO109I RGGNCCY 3 cut(s) 58, 421, 599
EcoRII CCWGG 1 cut(s) 627
EcoT14I CCWWGG 1 cut(s) 415
EcoT38I GRGCYC 1 cut(s) 195
EgeI GGCGCC 1 cut(s) 165
EheI GGCGCC 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 415
Esp3I CGTCTC 2 cut(s) 24, 864
FaeI CATG 4 cut(s) 124, 132, 373, 1043
FaqI GGGAC 2 cut(s) 44, 434
FatI CATG 4 cut(s) 120, 128, 369, 1039
FauNDI CATATG 2 cut(s) 533, 1058
Fnu4HI GCNGC 5 cut(s) 330, 348, 450, 523, 563
FokI GGATG 2 cut(s) 145, 868
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 5 cut(s) 330, 348, 450, 523, 563
FspBI CTAG 2 cut(s) 84, 606
FspI TGCGCA 1 cut(s) 951
GlaI GCGC 3 cut(s) 165, 448, 951
GluI GCNGC 5 cut(s) 330, 348, 450, 523, 563
GsuI CTGGAG 1 cut(s) 611
HaeII RGCGCY 2 cut(s) 167, 450
HaeIII GGCC 2 cut(s) 39, 342
HapII CCGG 6 cut(s) 68, 167, 177, 196, 381, 737
HgaI GACGC 1 cut(s) 605
HhaI GCGC 3 cut(s) 166, 449, 952
Hin1I GRCGYC 1 cut(s) 164
Hin1II CATG 4 cut(s) 124, 132, 373, 1043
Hin6I GCGC 3 cut(s) 164, 447, 950
HinP1I GCGC 3 cut(s) 164, 447, 950
HindIII AAGCTT 3 cut(s) 8, 291, 551
HinfI GANTC 8 cut(s) 278, 484, 742, 839, 905, 939, 969, 1036
HpaII CCGG 6 cut(s) 68, 167, 177, 196, 381, 737
HphI GGTGA 4 cut(s) 94, 254, 404, 448
Hpy166II GTNNAC 3 cut(s) 81, 262, 324
Hpy188I TCNGA 5 cut(s) 18, 144, 151, 406, 983
Hpy188III TCNNGA 5 cut(s) 43, 53, 488, 746, 1040
Hpy8I GTNNAC 3 cut(s) 81, 262, 324
Hpy99I CGWCG 2 cut(s) 337, 621
HpyAV CCTTC 2 cut(s) 130, 929
HpyCH4III ACNGT 6 cut(s) 49, 111, 259, 266, 321, 1121
HpyCH4IV ACGT 4 cut(s) 181, 811, 930, 1028
HpyCH4V TGCA 7 cut(s) 124, 316, 401, 525, 671, 678, 1001
HpyF10VI GCNNNNNNNGC 4 cut(s) 121, 299, 356, 559
HpyF3I CTNAG 6 cut(s) 141, 425, 502, 851, 903, 1074
HpySE526I ACGT 4 cut(s) 181, 811, 930, 1028
Hsp92I GRCGYC 1 cut(s) 164
Hsp92II CATG 4 cut(s) 124, 132, 373, 1043
HspAI GCGC 3 cut(s) 164, 447, 950
KasI GGCGCC 1 cut(s) 163
Kzo9I GATC 1 cut(s) 690
LmnI GCTCC 1 cut(s) 198
Lsp1109I GCAGC 1 cut(s) 509
LweI GCATC 3 cut(s) 410, 658, 687
MaeI CTAG 2 cut(s) 84, 606
MaeII ACGT 4 cut(s) 181, 811, 930, 1028
MaeIII GTNAC 3 cut(s) 100, 361, 376
MalI GATC 1 cut(s) 692
MboI GATC 1 cut(s) 690
MboII GAAGA 7 cut(s) 83, 227, 273, 700, 1034, 1045, 1120
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 3 cut(s) 285, 407, 830
Mly113I GGCGCC 1 cut(s) 164
MlyI GAGTC 4 cut(s) 833, 914, 933, 978
MmeI TCCRAC 1 cut(s) 174
MseI TTAA 3 cut(s) 186, 390, 1053
MslI CAYNNNNRTG 2 cut(s) 86, 881
MspA1I CMGCKG 2 cut(s) 329, 957
MspI CCGG 6 cut(s) 68, 167, 177, 196, 381, 737
MspR9I CCNGG 2 cut(s) 382, 629
Mva1269I GAATGC 1 cut(s) 565
MvaI CCWGG 1 cut(s) 629
MvnI CGCG 1 cut(s) 747
MwoI GCNNNNNNNGC 4 cut(s) 121, 299, 356, 559
NarI GGCGCC 1 cut(s) 164
NciI CCSGG 1 cut(s) 382
NdeI CATATG 2 cut(s) 533, 1058
NdeII GATC 1 cut(s) 690
NlaIII CATG 4 cut(s) 124, 132, 373, 1043
NlaIV GGNNCC 5 cut(s) 60, 165, 422, 639, 762
NmeAIII GCCGAG 1 cut(s) 478
NmuCI GTSAC 1 cut(s) 100
NruI TCGCGA 1 cut(s) 747
NsbI TGCGCA 1 cut(s) 951
NspI RCATGY 2 cut(s) 124, 373
OliI CACNNNNGTG 1 cut(s) 881
PaeR7I CTCGAG 3 cut(s) 41, 53, 431
PagI TCATGA 1 cut(s) 1039
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 1 cut(s) 565
PfeI GAWTC 4 cut(s) 278, 484, 742, 1036
PflMI CCANNNNNTGG 1 cut(s) 634
PkrI GCNGC 5 cut(s) 331, 349, 451, 524, 564
PleI GAGTC 4 cut(s) 833, 913, 933, 977
PluTI GGCGCC 1 cut(s) 167
PpsI GAGTC 4 cut(s) 833, 913, 933, 977
PpuMI RGGWCCY 3 cut(s) 58, 421, 599
Psp124BI GAGCTC 1 cut(s) 195
Psp1406I AACGTT 1 cut(s) 811
Psp5II RGGWCCY 3 cut(s) 58, 421, 599
Psp6I CCWGG 1 cut(s) 627
PspGI CCWGG 1 cut(s) 627
PspN4I GGNNCC 5 cut(s) 60, 165, 422, 639, 762
PspPI GGNCC 5 cut(s) 58, 421, 599, 637, 761
PspPPI RGGWCCY 3 cut(s) 58, 421, 599
PspXI VCTCGAGB 1 cut(s) 431
RruI TCGCGA 1 cut(s) 747
RsaI GTAC 4 cut(s) 308, 323, 828, 929
RsaNI GTAC 4 cut(s) 307, 322, 827, 928
RseI CAYNNNNRTG 2 cut(s) 86, 881
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 3 cut(s) 186, 390, 1053
SatI GCNGC 5 cut(s) 330, 348, 450, 523, 563
Sau3AI GATC 1 cut(s) 690
Sau96I GGNCC 5 cut(s) 58, 421, 599, 637, 761
SchI GAGTC 4 cut(s) 833, 914, 933, 978
ScrFI CCNGG 2 cut(s) 382, 629
SduI GDGCHC 1 cut(s) 195
SfaNI GCATC 3 cut(s) 410, 658, 687
SfoI GGCGCC 1 cut(s) 165
Sfr274I CTCGAG 3 cut(s) 41, 53, 431
SinI GGWCC 5 cut(s) 58, 421, 599, 637, 761
SlaI CTCGAG 3 cut(s) 41, 53, 431
SmiMI CAYNNNNRTG 2 cut(s) 86, 881
SmlI CTYRAG 5 cut(s) 41, 53, 431, 719, 841
SmoI CTYRAG 5 cut(s) 41, 53, 431, 719, 841
SpeI ACTAGT 1 cut(s) 83
Sse9I AATT 3 cut(s) 285, 407, 830
SsiI CCGC 5 cut(s) 329, 347, 450, 562, 957
SspDI GGCGCC 1 cut(s) 163
SspI AATATT 1 cut(s) 22
SspMI CTAG 2 cut(s) 84, 606
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 380, 627
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 6 cut(s) 49, 111, 259, 266, 321, 1121
TaiI ACGT 4 cut(s) 184, 814, 933, 1031
TaqI TCGA 4 cut(s) 42, 54, 432, 834
TasI AATT 3 cut(s) 285, 407, 830
TatI WGTACW 3 cut(s) 306, 321, 826
TauI GCSGC 4 cut(s) 332, 350, 452, 565
TfiI GAWTC 4 cut(s) 278, 484, 742, 1036
Tru1I TTAA 3 cut(s) 186, 390, 1053
Tru9I TTAA 3 cut(s) 186, 390, 1053
TscAI CASTG 1 cut(s) 907
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 1 cut(s) 522
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 4 cut(s) 149, 703, 901, 1028
TspRI CASTG 1 cut(s) 907
Van91I CCANNNNNTGG 1 cut(s) 634
VpaK11BI GGWCC 5 cut(s) 58, 421, 599, 637, 761
XapI RAATTY 1 cut(s) 407
XceI RCATGY 2 cut(s) 124, 373
XhoI CTCGAG 3 cut(s) 41, 53, 431
XspI CTAG 2 cut(s) 84, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.