FvH4_1g00010

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010233
Physical Location & Seq
Reverse (-)
7152 .. 13012
5861 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00010.t1

Sequence Viewer

Length: 921 bp
ATGGAGAACCACAACGCGAAATTGGTCCCAAGCGGTGGACTCAAACCTAACCCCGGCGAACAAGAAGAGAACCCTACTCTTAATCGTTGCGGAGAAGAAGAAGAAGTAAATACCGAATCGGCGGAGCCTCCTTCACCTAATGCCCCGCAGTCCTCAAGAACTCCCTTCACTAATCTCAGTCAGGTCGATGCTGATCTTGCTCTTGCTCGAACTCTCCAAGAACAGGAAAGGGCATACATGATGCTGAGAATGAACAATGAGGGAAGTGATTATGGCAGTTGGGAAGCTGGAAGTTATGTGCATGATGATGAGGATGATTTTCATGATGATACTGACGGAAATGACGATGATGAGCATTATGATGGAACTGATGCTGACAATGATGAAGAAGATGCATTTGATGTGCACGCTCATGAAGATGCTGGAGACGATCATAACCCCAGTGTGGTATATGATCCTGCTGTTTTTTCAAGTGATGAAGCGTATGCAAGAGCCCTGCAGGATGCTGAAGATAGAGATATGGCAGCTAGACTGATGGCCTTTGCAGGGATTGATGACCGTGAAGCTGAGGACACAGAGAATCAGGGAGGTAACTCTCAGGATGCATGGGAGGAGGTTGACCCAGATGAACTTTCATATGAGGAGTTACTTGCGTTGGGTGAAGTTGTTGGAACAGAGAGCAGAGGACTTCCTGCTGATACAATTGCCTCATTGCCTTCAGTAAGCTACAAGATGGGAAATGGTCAGAATGGAAGCAATGAGTCGTGTGCCATTTGTCGGTTAGATTATGAGGATGGTGAAAACTTGACAGTACTTTCTTGCACACACTCTTATCATTCTGAGTGCATAAATAATTGGTTGACAATAAACAAGGTCTGCCCAGTTTGCATTGCTGAAGTCTCCACGTCCGGAAACAGCTAG

Protein Analysis

307

Amino Acids

33.47

Weight (kDa)

4.05

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 253 - 296 9.2e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 255 - 296 2.7e-12 Ring finger domain
zf-C3HC4_2 PF13923 256 - 296 3.9e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 256 - 283 4.5e-08 RING-like zinc finger
zf-C3HC4 PF00097 256 - 296 6.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 256 - 296 4.5e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 35
AccII CGCG 1 cut(s) 17
AccIII TCCGGA 1 cut(s) 908
AciI CCGC 4 cut(s) 33, 90, 122, 146
AclWI GGATC 1 cut(s) 449
AcuI CTGAAG 3 cut(s) 528, 702, 915
AfaI GTAC 1 cut(s) 813
AfiI CCNNNNNNNGG 6 cut(s) 35, 53, 223, 445, 546, 777
AgsI TTSAA 1 cut(s) 471
AjiI CACGTC 1 cut(s) 906
AluBI AGCT 5 cut(s) 287, 527, 566, 726, 918
AluI AGCT 5 cut(s) 287, 527, 566, 726, 918
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 2 cut(s) 420, 904
Alw44I GTGCAC 1 cut(s) 404
AlwI GGATC 1 cut(s) 449
Aor13HI TCCGGA 1 cut(s) 908
AoxI GGCC 1 cut(s) 537
ApaLI GTGCAC 1 cut(s) 404
ApeKI GCWGC 1 cut(s) 524
AspS9I GGNCC 1 cut(s) 25
AsuC2I CCSGG 1 cut(s) 54
AsuHPI GGTGA 3 cut(s) 126, 671, 809
AvaII GGWCC 1 cut(s) 25
BaeGI GKGCMC 1 cut(s) 408
BanII GRGCYC 1 cut(s) 496
Bbv12I GWGCWC 1 cut(s) 408
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 1 cut(s) 536
BccI CCATC 4 cut(s) 356, 529, 727, 788
BcnI CCSGG 1 cut(s) 54
BcoDI GTCTC 2 cut(s) 420, 904
BfaI CTAG 2 cut(s) 528, 919
BfmI CTRYAG 1 cut(s) 497
BisI GCNGC 1 cut(s) 525
BlsI GCNGC 1 cut(s) 526
BmcAI AGTACT 1 cut(s) 813
Bme1390I CCNGG 1 cut(s) 54
Bme18I GGWCC 1 cut(s) 25
BmgBI CACGTC 1 cut(s) 906
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 2 cut(s) 27, 126
BmrFI CCNGG 1 cut(s) 54
BmrI ACTGGG 2 cut(s) 435, 875
BmsI GCATC 7 cut(s) 178, 231, 361, 382, 409, 493, 592
BmuI ACTGGG 2 cut(s) 435, 875
BpmI CTGGAG 1 cut(s) 444
Bpu10I CCTNAGC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 139
BpuMI CCSGG 1 cut(s) 54
BsaBI GATNNNNATC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 52
BsaWI WCCGGW 1 cut(s) 908
Bsc4I CCNNNNNNNGG 6 cut(s) 35, 53, 223, 445, 546, 777
Bse1I ACTGG 2 cut(s) 441, 881
Bse3DI GCAATG 3 cut(s) 710, 763, 888
Bse8I GATNNNNATC 1 cut(s) 192
BseAI TCCGGA 1 cut(s) 908
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 4 cut(s) 319, 508, 607, 799
BseJI GATNNNNATC 1 cut(s) 192
BseLI CCNNNNNNNGG 6 cut(s) 35, 53, 223, 445, 546, 777
BseMI GCAATG 3 cut(s) 710, 763, 888
BseMII CTCAG 5 cut(s) 190, 236, 558, 611, 831
BseNI ACTGG 2 cut(s) 441, 881
BseRI GAGGAG 2 cut(s) 626, 656
BseSI GKGCMC 1 cut(s) 408
BseXI GCAGC 1 cut(s) 536
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 539
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 2 cut(s) 54, 909
BslFI GGGAC 1 cut(s) 11
BslI CCNNNNNNNGG 6 cut(s) 35, 53, 223, 445, 546, 777
BsmAI GTCTC 2 cut(s) 420, 904
BsmBI CGTCTC 1 cut(s) 420
BsmFI GGGAC 1 cut(s) 11
BsnI GGCC 1 cut(s) 539
Bsp1286I GDGCHC 2 cut(s) 408, 496
Bsp13I TCCGGA 1 cut(s) 908
Bsp143I GATC 3 cut(s) 193, 430, 454
BspACI CCGC 4 cut(s) 33, 90, 122, 146
BspANI GGCC 1 cut(s) 539
BspCNI CTCAG 5 cut(s) 189, 237, 559, 610, 832
BspEI TCCGGA 1 cut(s) 908
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 2 cut(s) 322, 412
BspLI GGNNCC 2 cut(s) 27, 126
BspMAI CTGCAG 1 cut(s) 501
BspPI GGATC 1 cut(s) 449
BsrDI GCAATG 3 cut(s) 710, 763, 888
BsrI ACTGG 2 cut(s) 441, 881
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 3 cut(s) 193, 430, 454
Bst4CI ACNGT 2 cut(s) 560, 811
Bst6I CTCTTC 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 5 cut(s) 176, 245, 567, 597, 840
BstF5I GGATG 4 cut(s) 319, 508, 607, 799
BstFNI CGCG 1 cut(s) 17
BstKTI GATC 3 cut(s) 196, 433, 457
BstMAI GTCTC 2 cut(s) 420, 904
BstMBI GATC 3 cut(s) 193, 430, 454
BstMWI GCNNNNNNNGC 2 cut(s) 197, 885
BstSCI CCNGG 1 cut(s) 52
BstSFI CTRYAG 1 cut(s) 497
BstSLI GKGCMC 1 cut(s) 408
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 1 cut(s) 536
BsuRI GGCC 1 cut(s) 539
BtrI CACGTC 1 cut(s) 906
BtsCI GGATG 4 cut(s) 319, 508, 607, 799
BtsIMutI CAGTG 1 cut(s) 448
Cac8I GCNNGC 1 cut(s) 408
CciI TCATGA 2 cut(s) 322, 412
Cfr13I GGNCC 1 cut(s) 25
Csp6I GTAC 1 cut(s) 812
CviAII CATG 5 cut(s) 238, 302, 323, 413, 606
CviJI RGCY 8 cut(s) 127, 287, 494, 527, 539, 566, 726, 918
CviKI_1 RGCY 8 cut(s) 127, 287, 494, 527, 539, 566, 726, 918
CviQI GTAC 1 cut(s) 812
DdeI CTNAG 5 cut(s) 176, 245, 567, 597, 840
DpnI GATC 3 cut(s) 195, 432, 456
DpnII GATC 3 cut(s) 193, 430, 454
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
EciI GGCGGA 1 cut(s) 137
Eco24I GRGCYC 1 cut(s) 496
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 3 cut(s) 528, 702, 915
EcoT22I ATGCAT 2 cut(s) 397, 607
EcoT38I GRGCYC 1 cut(s) 496
Esp3I CGTCTC 1 cut(s) 420
FaeI CATG 5 cut(s) 241, 305, 326, 416, 609
FaqI GGGAC 1 cut(s) 11
FatI CATG 5 cut(s) 237, 301, 322, 412, 605
FauI CCCGC 1 cut(s) 153
FauNDI CATATG 1 cut(s) 637
Fnu4HI GCNGC 1 cut(s) 525
FokI GGATG 4 cut(s) 326, 515, 614, 806
FriOI GRGCYC 1 cut(s) 496
Fsp4HI GCNGC 1 cut(s) 525
FspBI CTAG 2 cut(s) 528, 919
GluI GCNGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 1 cut(s) 539
HapII CCGG 2 cut(s) 54, 909
Hin1II CATG 5 cut(s) 241, 305, 326, 416, 609
HincII GTYRAC 2 cut(s) 619, 861
HindII GTYRAC 2 cut(s) 619, 861
HinfI GANTC 4 cut(s) 39, 116, 580, 761
HpaII CCGG 2 cut(s) 54, 909
HphI GGTGA 3 cut(s) 126, 671, 809
Hpy166II GTNNAC 4 cut(s) 38, 406, 619, 861
Hpy188I TCNGA 2 cut(s) 747, 841
Hpy188III TCNNGA 5 cut(s) 156, 323, 413, 599, 909
Hpy8I GTNNAC 4 cut(s) 38, 406, 619, 861
HpyAV CCTTC 3 cut(s) 141, 175, 726
HpyCH4III ACNGT 2 cut(s) 560, 811
HpyCH4IV ACGT 1 cut(s) 905
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 885
HpyF3I CTNAG 5 cut(s) 176, 245, 567, 597, 840
HpySE526I ACGT 1 cut(s) 905
Hsp92II CATG 5 cut(s) 241, 305, 326, 416, 609
Kpn2I TCCGGA 1 cut(s) 908
Kzo9I GATC 3 cut(s) 193, 430, 454
LmnI GCTCC 1 cut(s) 124
Lsp1109I GCAGC 1 cut(s) 536
LweI GCATC 7 cut(s) 178, 231, 361, 382, 409, 493, 592
MaeI CTAG 2 cut(s) 528, 919
MaeII ACGT 1 cut(s) 905
MaeIII GTNAC 2 cut(s) 590, 645
MalI GATC 3 cut(s) 195, 432, 456
MboI GATC 3 cut(s) 193, 430, 454
MboII GAAGA 8 cut(s) 77, 107, 110, 113, 398, 401, 428, 521
MfeI CAATTG 1 cut(s) 702
MhlI GDGCHC 2 cut(s) 408, 496
MluCI AATT 3 cut(s) 20, 702, 853
MlyI GAGTC 2 cut(s) 33, 770
MmeI TCCRAC 1 cut(s) 649
Mph1103I ATGCAT 2 cut(s) 397, 607
MroI TCCGGA 1 cut(s) 908
MseI TTAA 1 cut(s) 81
MslI CAYNNNNRTG 4 cut(s) 306, 360, 411, 417
MspI CCGG 2 cut(s) 54, 909
MspR9I CCNGG 1 cut(s) 54
MunI CAATTG 1 cut(s) 702
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 2 cut(s) 197, 885
NciI CCSGG 1 cut(s) 54
NdeI CATATG 1 cut(s) 637
NdeII GATC 3 cut(s) 193, 430, 454
NlaIII CATG 5 cut(s) 241, 305, 326, 416, 609
NlaIV GGNNCC 2 cut(s) 27, 126
NsiI ATGCAT 2 cut(s) 397, 607
PagI TCATGA 2 cut(s) 322, 412
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PfeI GAWTC 2 cut(s) 116, 580
PflMI CCANNNNNTGG 1 cut(s) 35
PkrI GCNGC 1 cut(s) 526
PleI GAGTC 2 cut(s) 33, 769
PpsI GAGTC 2 cut(s) 33, 769
PspN4I GGNNCC 2 cut(s) 27, 126
PspPI GGNCC 1 cut(s) 25
PstI CTGCAG 1 cut(s) 501
RsaI GTAC 1 cut(s) 813
RsaNI GTAC 1 cut(s) 812
RseI CAYNNNNRTG 4 cut(s) 306, 360, 411, 417
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 3 cut(s) 193, 430, 454
Sau96I GGNCC 1 cut(s) 25
SbfI CCTGCAGG 1 cut(s) 501
ScaI AGTACT 1 cut(s) 813
SchI GAGTC 2 cut(s) 33, 770
ScrFI CCNGG 1 cut(s) 54
SdaI CCTGCAGG 1 cut(s) 501
SduI GDGCHC 2 cut(s) 408, 496
SfaNI GCATC 7 cut(s) 178, 231, 361, 382, 409, 493, 592
SfcI CTRYAG 1 cut(s) 497
SinI GGWCC 1 cut(s) 25
SmiMI CAYNNNNRTG 4 cut(s) 306, 360, 411, 417
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
Sse8387I CCTGCAGG 1 cut(s) 501
Sse9I AATT 3 cut(s) 20, 702, 853
SsiI CCGC 4 cut(s) 33, 90, 122, 146
SspMI CTAG 2 cut(s) 528, 919
StyD4I CCNGG 1 cut(s) 52
TaaI ACNGT 2 cut(s) 560, 811
TaiI ACGT 1 cut(s) 908
TaqI TCGA 2 cut(s) 186, 208
TasI AATT 3 cut(s) 20, 702, 853
TatI WGTACW 1 cut(s) 811
TfiI GAWTC 2 cut(s) 116, 580
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TscAI CASTG 1 cut(s) 448
TseI GCWGC 1 cut(s) 524
TspDTI ATGAA 7 cut(s) 266, 311, 399, 429, 492, 624, 642
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 1 cut(s) 448
Van91I CCANNNNNTGG 1 cut(s) 35
VneI GTGCAC 1 cut(s) 404
VpaK11BI GGWCC 1 cut(s) 25
XspI CTAG 2 cut(s) 528, 919
ZrmI AGTACT 1 cut(s) 813
Zsp2I ATGCAT 2 cut(s) 397, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.