RLG00000015601

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010233
Physical Location & Seq
Forward (+)
71189 .. 77354
6166 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015601

Sequence Viewer

Length: 945 bp
ATGGAGAACCACAACGCGAAATTGGTCCCTAACGGTGGACTCAAACCTAACCCCAGCAACAACCTTAGCGAACAAGAACAAGAAGAGAACCCTACTCTTAATCGTTGCGGAGAAGAAGAAGAAGAAGAAGAAGTAACTACGCAATTGGCGGAGCCTCCTTCACCTAATGCCCCGCAGTCCTCAAGAACTCCCTTCACTAATCTCAGTCAGGTCGATGCTGACCTTGCTCTTGCTCGAACTCTCCAAGAACAGGAAAGGGCATACATGATGCTGCGGATGAACAATGAAGGGAGTGATTATGGCAGTTGGGAAGCTGGAAGTTATGTGCATGATGATGAGGATGATTTTCATGATGATACTGACGGAAATGATGATGAGGAGCAATATGATGGGACTGATGCTGACAATGATGAAGAAGATGCATTTGATGTGCACGCTCATGATGATGCTGGAGACGATCATAACCCCAGTGTCGTATATGACCCTGCTGTTTTTTCAAGTGATGAGGCGTATGCAAGAGCCCTGCAGGATGCTGAAGATAGAGATATGGCAGCTAGACTGATGGCCCTTGCAATTGATGACCGTGAAGCTGAGGACACTGAGGATCAGGGAGGTAACTCTCAGGATGCATGGGAGGAGGTTGACCCAGATGAGCTTTCGTATGAGGAGTTACTTGCATTGGGTGAAGTTGTTGGAACAGAGAGCAGAGGGCTTTCTGCTGATACAATTGCTTCATTGCCTTCAGTAAGCTACAAGATGGGCAGTGATCAGAATGGAAGCAATGAGTCGTGTGTCATTTGTCGGTTAGATTATGAGGATGGTGAAAACTTGACAGTACTTTCTTGCAAACACTCTTATCATTCTGATTGCATAAATAATTGGTTGACAATAAACAAGGTCTGCCCAGTTTGCAGTGCTGAAGTCTCCACATCTGGAAACAGCTAG

Protein Analysis

315

Amino Acids

34.55

Weight (kDa)

4.05

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 259 - 304 1.1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 263 - 304 5.1e-13 Ring finger domain
zf-C3HC4_2 PF13923 264 - 304 3.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 264 - 291 4.3e-08 RING-like zinc finger
zf-C3HC4 PF00097 264 - 304 5.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 264 - 305 1.8e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 17
AciI CCGC 4 cut(s) 108, 149, 173, 274
AclWI GGATC 1 cut(s) 612
AcuI CTGAAG 3 cut(s) 555, 726, 939
AfaI GTAC 1 cut(s) 837
AfiI CCNNNNNNNGG 2 cut(s) 35, 250
AgsI TTSAA 1 cut(s) 498
AluBI AGCT 6 cut(s) 314, 554, 590, 655, 750, 942
AluI AGCT 6 cut(s) 314, 554, 590, 655, 750, 942
Alw21I GWGCWC 1 cut(s) 435
Alw26I GTCTC 2 cut(s) 447, 928
Alw44I GTGCAC 1 cut(s) 431
AlwI GGATC 1 cut(s) 612
AoxI GGCC 1 cut(s) 564
ApaLI GTGCAC 1 cut(s) 431
ApeKI GCWGC 2 cut(s) 271, 551
AspS9I GGNCC 2 cut(s) 25, 565
AsuHPI GGTGA 3 cut(s) 153, 695, 833
AvaII GGWCC 1 cut(s) 25
BaeGI GKGCMC 1 cut(s) 435
BanII GRGCYC 1 cut(s) 523
Bbv12I GWGCWC 1 cut(s) 435
BbvCI CCTCAGC 1 cut(s) 591
BbvI GCAGC 2 cut(s) 258, 563
BccI CCATC 4 cut(s) 383, 556, 751, 812
BclI TGATCA 1 cut(s) 766
BcoDI GTCTC 2 cut(s) 447, 928
BfaI CTAG 2 cut(s) 555, 943
BfmI CTRYAG 1 cut(s) 524
BisI GCNGC 2 cut(s) 272, 552
BlsI GCNGC 2 cut(s) 273, 553
BmcAI AGTACT 1 cut(s) 837
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 2 cut(s) 25, 565
BmiI GGNNCC 2 cut(s) 27, 153
BmrI ACTGGG 2 cut(s) 462, 899
BmsI GCATC 7 cut(s) 205, 258, 388, 409, 436, 520, 616
BmuI ACTGGG 2 cut(s) 462, 899
BpmI CTGGAG 1 cut(s) 471
Bpu10I CCTNAGC 2 cut(s) 65, 591
BpuEI CTTGAG 1 cut(s) 166
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 250
Bse1I ACTGG 2 cut(s) 468, 905
Bse3DI GCAATG 2 cut(s) 734, 787
BseGI GGATG 5 cut(s) 282, 346, 535, 631, 823
BseLI CCNNNNNNNGG 2 cut(s) 35, 250
BseMI GCAATG 2 cut(s) 734, 787
BseMII CTCAG 4 cut(s) 217, 582, 591, 635
BseNI ACTGG 2 cut(s) 468, 905
BseRI GAGGAG 3 cut(s) 392, 650, 680
BseSI GKGCMC 1 cut(s) 435
BseXI GCAGC 2 cut(s) 258, 563
BseYI CCCAGC 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 566
BsiHKAI GWGCWC 1 cut(s) 435
BslFI GGGAC 2 cut(s) 11, 406
BslI CCNNNNNNNGG 2 cut(s) 35, 250
BsmAI GTCTC 2 cut(s) 447, 928
BsmBI CGTCTC 1 cut(s) 447
BsmFI GGGAC 2 cut(s) 11, 406
BsnI GGCC 1 cut(s) 566
Bsp1286I GDGCHC 2 cut(s) 435, 523
Bsp143I GATC 3 cut(s) 457, 604, 766
BspACI CCGC 4 cut(s) 108, 149, 173, 274
BspANI GGCC 1 cut(s) 566
BspCNI CTCAG 4 cut(s) 216, 583, 592, 634
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 2 cut(s) 349, 439
BspLI GGNNCC 2 cut(s) 27, 153
BspMAI CTGCAG 1 cut(s) 528
BspPI GGATC 1 cut(s) 612
BsrDI GCAATG 2 cut(s) 734, 787
BsrI ACTGG 2 cut(s) 468, 905
BssMI GATC 3 cut(s) 457, 604, 766
Bst4CI ACNGT 3 cut(s) 35, 584, 835
Bst6I CTCTTC 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 5 cut(s) 65, 203, 591, 600, 621
BstF5I GGATG 5 cut(s) 282, 346, 535, 631, 823
BstFNI CGCG 1 cut(s) 17
BstKTI GATC 3 cut(s) 460, 607, 769
BstMAI GTCTC 2 cut(s) 447, 928
BstMBI GATC 3 cut(s) 457, 604, 766
BstMWI GCNNNNNNNGC 2 cut(s) 224, 909
BstSFI CTRYAG 1 cut(s) 524
BstSLI GKGCMC 1 cut(s) 435
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 2 cut(s) 258, 563
BsuRI GGCC 1 cut(s) 566
BtsCI GGATG 5 cut(s) 282, 346, 535, 631, 823
BtsI GCAGTG 2 cut(s) 769, 919
BtsIMutI CAGTG 4 cut(s) 475, 597, 769, 919
Cac8I GCNNGC 1 cut(s) 435
CciI TCATGA 2 cut(s) 349, 439
Cfr13I GGNCC 2 cut(s) 25, 565
Csp6I GTAC 1 cut(s) 836
CviAII CATG 5 cut(s) 265, 329, 350, 440, 630
CviQI GTAC 1 cut(s) 836
DdeI CTNAG 5 cut(s) 65, 203, 591, 600, 621
DpnI GATC 3 cut(s) 459, 606, 768
DpnII GATC 3 cut(s) 457, 604, 766
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
EciI GGCGGA 1 cut(s) 164
Eco24I GRGCYC 1 cut(s) 523
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 3 cut(s) 555, 726, 939
EcoT22I ATGCAT 2 cut(s) 424, 631
EcoT38I GRGCYC 1 cut(s) 523
Esp3I CGTCTC 1 cut(s) 447
FaeI CATG 5 cut(s) 268, 332, 353, 443, 633
FaqI GGGAC 2 cut(s) 11, 406
FatI CATG 5 cut(s) 264, 328, 349, 439, 629
FauI CCCGC 1 cut(s) 180
FbaI TGATCA 1 cut(s) 766
Fnu4HI GCNGC 2 cut(s) 272, 552
FokI GGATG 5 cut(s) 289, 353, 542, 638, 830
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 2 cut(s) 272, 552
FspBI CTAG 2 cut(s) 555, 943
GluI GCNGC 2 cut(s) 272, 552
GsaI CCCAGC 1 cut(s) 57
GsuI CTGGAG 1 cut(s) 471
HaeIII GGCC 1 cut(s) 566
Hin1II CATG 5 cut(s) 268, 332, 353, 443, 633
HincII GTYRAC 2 cut(s) 643, 885
HindII GTYRAC 2 cut(s) 643, 885
HinfI GANTC 2 cut(s) 39, 785
HphI GGTGA 3 cut(s) 153, 695, 833
Hpy166II GTNNAC 4 cut(s) 38, 433, 643, 885
Hpy188I TCNGA 2 cut(s) 771, 865
Hpy188III TCNNGA 5 cut(s) 183, 350, 440, 623, 933
Hpy8I GTNNAC 4 cut(s) 38, 433, 643, 885
HpyAV CCTTC 4 cut(s) 168, 202, 281, 750
HpyCH4III ACNGT 3 cut(s) 35, 584, 835
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 909
HpyF3I CTNAG 5 cut(s) 65, 203, 591, 600, 621
Hsp92II CATG 5 cut(s) 268, 332, 353, 443, 633
Ksp22I TGATCA 1 cut(s) 766
Kzo9I GATC 3 cut(s) 457, 604, 766
LmnI GCTCC 2 cut(s) 151, 379
Lsp1109I GCAGC 2 cut(s) 258, 563
LweI GCATC 7 cut(s) 205, 258, 388, 409, 436, 520, 616
MaeI CTAG 2 cut(s) 555, 943
MaeIII GTNAC 3 cut(s) 133, 614, 669
MalI GATC 3 cut(s) 459, 606, 768
MboI GATC 3 cut(s) 457, 604, 766
MfeI CAATTG 3 cut(s) 143, 573, 726
MhlI GDGCHC 2 cut(s) 435, 523
MluCI AATT 5 cut(s) 20, 143, 573, 726, 877
MlyI GAGTC 2 cut(s) 33, 794
MmeI TCCRAC 1 cut(s) 673
Mph1103I ATGCAT 2 cut(s) 424, 631
MseI TTAA 1 cut(s) 99
MslI CAYNNNNRTG 3 cut(s) 333, 438, 444
MunI CAATTG 3 cut(s) 143, 573, 726
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 2 cut(s) 224, 909
NdeII GATC 3 cut(s) 457, 604, 766
NlaIII CATG 5 cut(s) 268, 332, 353, 443, 633
NlaIV GGNNCC 2 cut(s) 27, 153
NsiI ATGCAT 2 cut(s) 424, 631
PagI TCATGA 2 cut(s) 349, 439
PkrI GCNGC 2 cut(s) 273, 553
PleI GAGTC 2 cut(s) 33, 793
PpsI GAGTC 2 cut(s) 33, 793
PspFI CCCAGC 1 cut(s) 53
PspN4I GGNNCC 2 cut(s) 27, 153
PspPI GGNCC 2 cut(s) 25, 565
PstI CTGCAG 1 cut(s) 528
RsaI GTAC 1 cut(s) 837
RsaNI GTAC 1 cut(s) 836
RseI CAYNNNNRTG 3 cut(s) 333, 438, 444
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 2 cut(s) 272, 552
Sau3AI GATC 3 cut(s) 457, 604, 766
Sau96I GGNCC 2 cut(s) 25, 565
SbfI CCTGCAGG 1 cut(s) 528
ScaI AGTACT 1 cut(s) 837
SchI GAGTC 2 cut(s) 33, 794
SdaI CCTGCAGG 1 cut(s) 528
SduI GDGCHC 2 cut(s) 435, 523
SfaNI GCATC 7 cut(s) 205, 258, 388, 409, 436, 520, 616
SfcI CTRYAG 1 cut(s) 524
SinI GGWCC 1 cut(s) 25
SmiMI CAYNNNNRTG 3 cut(s) 333, 438, 444
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
Sse8387I CCTGCAGG 1 cut(s) 528
Sse9I AATT 5 cut(s) 20, 143, 573, 726, 877
SsiI CCGC 4 cut(s) 108, 149, 173, 274
SspMI CTAG 2 cut(s) 555, 943
TaaI ACNGT 3 cut(s) 35, 584, 835
TaqI TCGA 2 cut(s) 213, 235
TasI AATT 5 cut(s) 20, 143, 573, 726, 877
TatI WGTACW 1 cut(s) 835
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 4 cut(s) 475, 604, 769, 919
TseI GCWGC 2 cut(s) 271, 551
TspDTI ATGAA 5 cut(s) 293, 300, 338, 426, 723
TspGWI ACGGA 1 cut(s) 378
TspRI CASTG 4 cut(s) 475, 604, 769, 919
VneI GTGCAC 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 25
XspI CTAG 2 cut(s) 555, 943
ZrmI AGTACT 1 cut(s) 837
Zsp2I ATGCAT 2 cut(s) 424, 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.