MD00G1145000.v1.1

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0010233
Physical Location & Seq
Reverse (-)
31721006 .. 31725679
4674 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1145000.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGAGAATGATGACGTCAAATTGGGGTCTGCCGGCGGACTCAAATCCAACAACAATAAGAACGTCAGCGAACAAGAACAAAACCCTTGTTTGAATGAATGCGAAGAACGAGGAGAAGAAGTAAATAGTGAGCCTTCGCAGGAACAGGAGGTGGATCCTTCCTCCTCACCTCCTGTTCCCCTGCCTTCAAGAACTCCCTTCAGTAATCTCAGTCAGGTCGATGCCGACCTAGCCCTTGCTCGAACCCTCCAAGAACAGGAAAGGGCATACATGATGCTACGAATGAACAATGAAGGTAGTGATTATGGAAGTTGGGAAGCTGGAAGTTATGTGCATGAGGATGATAATGATTTCAATGAATTTGACGACGATGCTGATGAGGAACAATATGATGGAACTGAGGTTGACAGTGATGAAGATGCATTTGATTTGCATGCTCAAGATGATGCTGGAGAGGATGATAACCCCAGTGTCGAATATGGTCCGGCTGTTTTTTCAAGTGATGAGGCCTATGCAAGAGCCCTGCAAGATGCTGAAGACAGAGAGATGGCCGCTAGACTGATGGCACTTTCTGGGATCAATGACCGTGAAGTAGAGGACACAGAGGAACATGGTGGTAACTCTCAGGATACATGGGAGGAGATTGACCCAGATGAACTTTCATATGAGGAGTTACTTGCATTGGGCGAAGTTGTTGGAACTGAGAACAGAGGGCTCTCAGCTGATACTATCGCCTCATTGCCTTCAGTAAGCTACAAGACAGGAAGCAGCCAGAATGGGAGCAATGAGTCGTGTGTCATTTGTCGGTTGGACTTCGAGGATGGTGAGAATTTGACAATACTATCCTGCAAACATTCCTATCATTCTGAGTGCATAAATAATTGGTTGACAATAAACAAGATCTGCCCTGTTTGCAGCGCTGAAGTTTCCACATCTGGAAACAGCTAG

Protein Analysis

316

Amino Acids

34.62

Weight (kDa)

4.05

Isoelectric Point (pI)

52.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 258 - 305 2.7e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 264 - 305 5e-13 Ring finger domain
zf-RING_11 PF17123 265 - 292 3e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 265 - 305 9.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 265 - 306 2.4e-06 zinc-RING finger domain
zf-C3HC4 PF00097 265 - 305 3.5e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 18
AciI CCGC 2 cut(s) 36, 552
AclWI GGATC 3 cut(s) 149, 162, 584
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 2 cut(s) 359, 829
AcuI CTGAAG 4 cut(s) 184, 555, 729, 942
AcyI GRCGYC 1 cut(s) 15
AfeI AGCGCT 1 cut(s) 919
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 4 cut(s) 94, 189, 355, 498
AluBI AGCT 4 cut(s) 320, 722, 753, 945
AluI AGCT 4 cut(s) 320, 722, 753, 945
AlwI GGATC 3 cut(s) 149, 162, 584
Aor51HI AGCGCT 1 cut(s) 919
AoxI GGCC 2 cut(s) 507, 549
ApeKI GCWGC 2 cut(s) 768, 915
ApoI RAATTY 2 cut(s) 359, 829
AspLEI GCGC 1 cut(s) 920
AspS9I GGNCC 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 159, 836
AvaII GGWCC 1 cut(s) 482
BamHI GGATCC 1 cut(s) 154
BanII GRGCYC 2 cut(s) 523, 717
BbsI GAAGAC 1 cut(s) 543
BbvI GCAGC 2 cut(s) 780, 927
BccI CCATC 4 cut(s) 386, 541, 556, 815
BciVI GTATCC 1 cut(s) 622
BfaI CTAG 3 cut(s) 230, 555, 946
BfoI RGCGCY 1 cut(s) 921
BfuI GTATCC 1 cut(s) 622
BglII AGATCT 1 cut(s) 900
BisI GCNGC 3 cut(s) 552, 769, 916
BlsI GCNGC 3 cut(s) 553, 770, 917
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 1 cut(s) 482
BmiI GGNNCC 1 cut(s) 156
BmrI ACTGGG 1 cut(s) 462
BmsI GCATC 6 cut(s) 211, 264, 361, 409, 436, 520
BmuI ACTGGG 1 cut(s) 462
BpiI GAAGAC 1 cut(s) 543
BpmI CTGGAG 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 15
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 468
Bse3DI GCAATG 2 cut(s) 737, 790
BseGI GGATG 3 cut(s) 346, 463, 826
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMI GCAATG 2 cut(s) 737, 790
BseMII CTCAG 6 cut(s) 223, 390, 638, 693, 732, 858
BseNI ACTGG 1 cut(s) 468
BseRI GAGGAG 4 cut(s) 126, 154, 653, 683
BseXI GCAGC 2 cut(s) 780, 927
BshFI GGCC 2 cut(s) 509, 551
BsiSI CCGG 2 cut(s) 33, 485
BslI CCNNNNNNNGG 1 cut(s) 256
BsmI GAATGC 1 cut(s) 104
BsnI GGCC 2 cut(s) 509, 551
Bsp1286I GDGCHC 2 cut(s) 523, 717
Bsp143I GATC 3 cut(s) 154, 576, 900
BspACI CCGC 2 cut(s) 36, 552
BspANI GGCC 2 cut(s) 509, 551
BspCNI CTCAG 6 cut(s) 222, 391, 637, 694, 731, 859
BspLI GGNNCC 1 cut(s) 156
BspPI GGATC 3 cut(s) 149, 162, 584
BsrDI GCAATG 2 cut(s) 737, 790
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 1 cut(s) 468
BssAI RCCGGY 1 cut(s) 32
BssMI GATC 3 cut(s) 154, 576, 900
BssNI GRCGYC 1 cut(s) 15
Bst4CI ACNGT 2 cut(s) 410, 587
BstACI GRCGYC 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 34, 435
BstDEI CTNAG 6 cut(s) 209, 399, 624, 702, 718, 867
BstF5I GGATG 3 cut(s) 346, 463, 826
BstH2I RGCGCY 1 cut(s) 921
BstHHI GCGC 1 cut(s) 920
BstKTI GATC 3 cut(s) 157, 579, 903
BstMBI GATC 3 cut(s) 154, 576, 900
BstMWI GCNNNNNNNGC 2 cut(s) 230, 912
BstNSI RCATGY 1 cut(s) 437
BstV1I GCAGC 2 cut(s) 780, 927
BstV2I GAAGAC 1 cut(s) 543
BstX2I RGATCY 2 cut(s) 154, 900
BstYI RGATCY 2 cut(s) 154, 900
BsuI GTATCC 1 cut(s) 622
BsuRI GGCC 2 cut(s) 509, 551
BtsCI GGATG 3 cut(s) 346, 463, 826
BtsIMutI CAGTG 2 cut(s) 415, 475
Cac8I GCNNGC 2 cut(s) 34, 435
CfoI GCGC 1 cut(s) 920
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 1 cut(s) 482
CviAII CATG 5 cut(s) 271, 335, 434, 611, 633
DdeI CTNAG 6 cut(s) 209, 399, 624, 702, 718, 867
DpnI GATC 3 cut(s) 156, 578, 902
DpnII GATC 3 cut(s) 154, 576, 900
EaeI YGGCCR 1 cut(s) 549
EciI GGCGGA 1 cut(s) 51
Eco147I AGGCCT 1 cut(s) 509
Eco24I GRGCYC 2 cut(s) 523, 717
Eco47I GGWCC 1 cut(s) 482
Eco47III AGCGCT 1 cut(s) 919
Eco57I CTGAAG 4 cut(s) 184, 555, 729, 942
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 523, 717
FaeI CATG 5 cut(s) 274, 338, 437, 614, 636
FatI CATG 5 cut(s) 270, 334, 433, 610, 632
FauNDI CATATG 1 cut(s) 664
Fnu4HI GCNGC 3 cut(s) 552, 769, 916
FokI GGATG 3 cut(s) 353, 470, 833
FriOI GRGCYC 2 cut(s) 523, 717
Fsp4HI GCNGC 3 cut(s) 552, 769, 916
FspBI CTAG 3 cut(s) 230, 555, 946
GlaI GCGC 1 cut(s) 919
GluI GCNGC 3 cut(s) 552, 769, 916
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 921
HaeIII GGCC 2 cut(s) 509, 551
HapII CCGG 2 cut(s) 33, 485
HhaI GCGC 1 cut(s) 920
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 5 cut(s) 274, 338, 437, 614, 636
Hin6I GCGC 1 cut(s) 918
HinP1I GCGC 1 cut(s) 918
HincII GTYRAC 2 cut(s) 406, 888
HindII GTYRAC 2 cut(s) 406, 888
HinfI GANTC 2 cut(s) 39, 788
HpaII CCGG 2 cut(s) 33, 485
HphI GGTGA 2 cut(s) 159, 836
Hpy166II GTNNAC 2 cut(s) 406, 888
Hpy188I TCNGA 1 cut(s) 868
Hpy188III TCNNGA 4 cut(s) 189, 440, 626, 936
Hpy8I GTNNAC 2 cut(s) 406, 888
Hpy99I CGWCG 1 cut(s) 371
HpyAV CCTTC 6 cut(s) 144, 168, 195, 208, 287, 753
HpyCH4III ACNGT 2 cut(s) 410, 587
HpyCH4IV ACGT 2 cut(s) 15, 63
HpyCH4V TGCA 9 cut(s) 334, 422, 433, 515, 526, 680, 849, 873, 915
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 912
HpyF3I CTNAG 6 cut(s) 209, 399, 624, 702, 718, 867
HpySE526I ACGT 2 cut(s) 15, 63
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 5 cut(s) 274, 338, 437, 614, 636
HspAI GCGC 1 cut(s) 918
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Kzo9I GATC 3 cut(s) 154, 576, 900
LmnI GCTCC 1 cut(s) 780
Lsp1109I GCAGC 2 cut(s) 780, 927
LweI GCATC 6 cut(s) 211, 264, 361, 409, 436, 520
MaeI CTAG 3 cut(s) 230, 555, 946
MaeII ACGT 2 cut(s) 15, 63
MaeIII GTNAC 2 cut(s) 617, 672
MalI GATC 3 cut(s) 156, 578, 902
MboI GATC 3 cut(s) 154, 576, 900
MboII GAAGA 4 cut(s) 116, 128, 428, 548
MflI RGATCY 2 cut(s) 154, 900
MhlI GDGCHC 2 cut(s) 523, 717
MluCI AATT 4 cut(s) 20, 359, 829, 880
MlyI GAGTC 2 cut(s) 33, 797
MmeI TCCRAC 3 cut(s) 72, 676, 789
Mph1103I ATGCAT 1 cut(s) 424
MroNI GCCGGC 1 cut(s) 32
MslI CAYNNNNRTG 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 2 cut(s) 33, 485
Mva1269I GAATGC 1 cut(s) 104
MwoI GCNNNNNNNGC 2 cut(s) 230, 912
NaeI GCCGGC 1 cut(s) 34
NdeI CATATG 1 cut(s) 664
NdeII GATC 3 cut(s) 154, 576, 900
NgoMIV GCCGGC 1 cut(s) 32
NlaIII CATG 5 cut(s) 274, 338, 437, 614, 636
NlaIV GGNNCC 1 cut(s) 156
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 1 cut(s) 437
PaeI GCATGC 1 cut(s) 437
PceI AGGCCT 1 cut(s) 509
PctI GAATGC 1 cut(s) 104
PdiI GCCGGC 1 cut(s) 34
PkrI GCNGC 3 cut(s) 553, 770, 917
PleI GAGTC 2 cut(s) 33, 796
PpsI GAGTC 2 cut(s) 33, 796
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 1 cut(s) 482
PsuI RGATCY 2 cut(s) 154, 900
PvuII CAGCTG 1 cut(s) 722
RseI CAYNNNNRTG 1 cut(s) 339
SatI GCNGC 3 cut(s) 552, 769, 916
Sau3AI GATC 3 cut(s) 154, 576, 900
Sau96I GGNCC 1 cut(s) 482
SchI GAGTC 2 cut(s) 33, 797
SduI GDGCHC 2 cut(s) 523, 717
SfaNI GCATC 6 cut(s) 211, 264, 361, 409, 436, 520
SinI GGWCC 1 cut(s) 482
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 1 cut(s) 438
SmoI CTYRAG 1 cut(s) 438
SphI GCATGC 1 cut(s) 437
Sse9I AATT 4 cut(s) 20, 359, 829, 880
SseBI AGGCCT 1 cut(s) 509
SsiI CCGC 2 cut(s) 36, 552
SspMI CTAG 3 cut(s) 230, 555, 946
StuI AGGCCT 1 cut(s) 509
TaaI ACNGT 2 cut(s) 410, 587
TaiI ACGT 2 cut(s) 18, 66
TaqI TCGA 4 cut(s) 219, 241, 474, 816
TasI AATT 4 cut(s) 20, 359, 829, 880
TauI GCSGC 1 cut(s) 554
TscAI CASTG 2 cut(s) 415, 475
TseI GCWGC 2 cut(s) 768, 915
TspDTI ATGAA 7 cut(s) 111, 299, 306, 372, 429, 651, 669
TspRI CASTG 2 cut(s) 415, 475
VpaK11BI GGWCC 1 cut(s) 482
XapI RAATTY 2 cut(s) 359, 829
XceI RCATGY 1 cut(s) 437
XspI CTAG 3 cut(s) 230, 555, 946
ZraI GACGTC 1 cut(s) 16
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.