RchiOBHm_Chr2g0084221

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010233
Physical Location & Seq
Forward (+)
69163 .. 75058
5896 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45982

Sequence Viewer

Length: 942 bp
ATGGAGAACCACAACGCGAAATTGGTCCCTAACGGTGGACTCAAACCTAACCCCAGCAACAACCTTAGCGAACAAGAACAAGAAGAGAACCCTACTCTTAATCGTTGCGGAGAAGAAGAAGAAGAAGAAGTAACTACGCAATTGGCGGAGCCTCCTTCACCTAATGCCCCGCAGTCCTCAAGAACTCCCTTCACTAATCTCAGTCAGGTCGATGCTGACCTTGCTCTTGCTCGAACTCTCCAAGAACAGGAAAGGGCATACATGATGCTGCGGATGAACAATGAAGGGAGTGATTATGGCAGTTGGGAAGCTGGAAGTTATGTGCATGATGATGAGGATGATTTTCATGATGATACTGACGGAAATGATGATGATGAGCAATATGATGGGACTGATGCTGACAATGATGAAGAAGATGCATTTGATGTGCACGCTCATGATGATGCTGGAGACGATCATAACCCCAGTGTTGTATATGACCCTACTGTTTTTTCAAGTGATGAGGCGTATGCAAGAGCCCTGCAGGATGCTGAAGATAGAGATATGGCAGCTAGACTGATGGCCCTTGCAATTGATGACCGTGAAGCTGAGGACACTGAGGATCAGGGAGGTAACTCTCAGGATGCATGGGAGGAGGTTGACCCAGATGAGCTTTCGTATGAGGAGTTACTTGCATTGGGTGAAGTTGTTGGAACAGAGAGCAGAGGGCTTTCTGCTGATACAATTGCTTCATTGCCTTCAGTAAGCTACAAGATGGGGAGCGATCAGAATGGAAGCAATGAGTCGTGTGTCATTTGTCGGTTAGATTATGAGGATGGTGAAAACTTGACAGTACTTTCTTGCAAACACTCTTATCATTCTGATTGCATAAATAATTGGTTGACAATAAACAAGGTCTGCCCAGTTTGCAGTGCTGAAGTCTCCACATCTGGAAACAGCTAG

Protein Analysis

313

Amino Acids

34.44

Weight (kDa)

4.05

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 258 - 303 1.1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 262 - 303 5e-13 Ring finger domain
zf-C3HC4_2 PF13923 263 - 303 3.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 263 - 290 4.3e-08 RING-like zinc finger
zf-C3HC4 PF00097 263 - 303 5.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 263 - 304 1.8e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 17
AciI CCGC 4 cut(s) 108, 146, 170, 271
AclWI GGATC 1 cut(s) 609
AcuI CTGAAG 3 cut(s) 552, 723, 936
AfaI GTAC 1 cut(s) 834
AfiI CCNNNNNNNGG 2 cut(s) 35, 247
AgsI TTSAA 1 cut(s) 495
AluBI AGCT 6 cut(s) 311, 551, 587, 652, 747, 939
AluI AGCT 6 cut(s) 311, 551, 587, 652, 747, 939
Alw21I GWGCWC 1 cut(s) 432
Alw26I GTCTC 2 cut(s) 444, 925
Alw44I GTGCAC 1 cut(s) 428
AlwI GGATC 1 cut(s) 609
AoxI GGCC 1 cut(s) 561
ApaLI GTGCAC 1 cut(s) 428
ApeKI GCWGC 2 cut(s) 268, 548
AspS9I GGNCC 2 cut(s) 25, 562
AsuHPI GGTGA 3 cut(s) 150, 692, 830
AvaII GGWCC 1 cut(s) 25
BaeGI GKGCMC 1 cut(s) 432
BanII GRGCYC 1 cut(s) 520
Bbv12I GWGCWC 1 cut(s) 432
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 2 cut(s) 255, 560
BccI CCATC 4 cut(s) 380, 553, 748, 809
BcoDI GTCTC 2 cut(s) 444, 925
BfaI CTAG 2 cut(s) 552, 940
BfmI CTRYAG 1 cut(s) 521
BisI GCNGC 2 cut(s) 269, 549
BlsI GCNGC 2 cut(s) 270, 550
BmcAI AGTACT 1 cut(s) 834
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 2 cut(s) 25, 562
BmiI GGNNCC 2 cut(s) 27, 150
BmrI ACTGGG 2 cut(s) 459, 896
BmsI GCATC 7 cut(s) 202, 255, 385, 406, 433, 517, 613
BmuI ACTGGG 2 cut(s) 459, 896
BpmI CTGGAG 1 cut(s) 468
Bpu10I CCTNAGC 2 cut(s) 65, 588
BpuEI CTTGAG 1 cut(s) 163
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 247
Bse1I ACTGG 2 cut(s) 465, 902
Bse3DI GCAATG 2 cut(s) 731, 784
BseGI GGATG 5 cut(s) 279, 343, 532, 628, 820
BseLI CCNNNNNNNGG 2 cut(s) 35, 247
BseMI GCAATG 2 cut(s) 731, 784
BseMII CTCAG 4 cut(s) 214, 579, 588, 632
BseNI ACTGG 2 cut(s) 465, 902
BseRI GAGGAG 2 cut(s) 647, 677
BseSI GKGCMC 1 cut(s) 432
BseXI GCAGC 2 cut(s) 255, 560
BseYI CCCAGC 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 563
BsiHKAI GWGCWC 1 cut(s) 432
BslFI GGGAC 2 cut(s) 11, 403
BslI CCNNNNNNNGG 2 cut(s) 35, 247
BsmAI GTCTC 2 cut(s) 444, 925
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 2 cut(s) 11, 403
BsnI GGCC 1 cut(s) 563
Bsp1286I GDGCHC 2 cut(s) 432, 520
Bsp143I GATC 3 cut(s) 454, 601, 763
BspACI CCGC 4 cut(s) 108, 146, 170, 271
BspANI GGCC 1 cut(s) 563
BspCNI CTCAG 4 cut(s) 213, 580, 589, 631
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 2 cut(s) 346, 436
BspLI GGNNCC 2 cut(s) 27, 150
BspMAI CTGCAG 1 cut(s) 525
BspPI GGATC 1 cut(s) 609
BsrDI GCAATG 2 cut(s) 731, 784
BsrI ACTGG 2 cut(s) 465, 902
BssMI GATC 3 cut(s) 454, 601, 763
Bst4CI ACNGT 4 cut(s) 35, 487, 581, 832
Bst6I CTCTTC 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 5 cut(s) 65, 200, 588, 597, 618
BstF5I GGATG 5 cut(s) 279, 343, 532, 628, 820
BstFNI CGCG 1 cut(s) 17
BstKTI GATC 3 cut(s) 457, 604, 766
BstMAI GTCTC 2 cut(s) 444, 925
BstMBI GATC 3 cut(s) 454, 601, 763
BstMWI GCNNNNNNNGC 2 cut(s) 221, 906
BstSFI CTRYAG 1 cut(s) 521
BstSLI GKGCMC 1 cut(s) 432
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 2 cut(s) 255, 560
BsuRI GGCC 1 cut(s) 563
BtsCI GGATG 5 cut(s) 279, 343, 532, 628, 820
BtsI GCAGTG 1 cut(s) 916
BtsIMutI CAGTG 3 cut(s) 472, 594, 916
Cac8I GCNNGC 1 cut(s) 432
CciI TCATGA 2 cut(s) 346, 436
Cfr13I GGNCC 2 cut(s) 25, 562
Csp6I GTAC 1 cut(s) 833
CviAII CATG 5 cut(s) 262, 326, 347, 437, 627
CviQI GTAC 1 cut(s) 833
DdeI CTNAG 5 cut(s) 65, 200, 588, 597, 618
DpnI GATC 3 cut(s) 456, 603, 765
DpnII GATC 3 cut(s) 454, 601, 763
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
EciI GGCGGA 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 520
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 3 cut(s) 552, 723, 936
EcoT22I ATGCAT 2 cut(s) 421, 628
EcoT38I GRGCYC 1 cut(s) 520
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 5 cut(s) 265, 329, 350, 440, 630
FaqI GGGAC 2 cut(s) 11, 403
FatI CATG 5 cut(s) 261, 325, 346, 436, 626
FauI CCCGC 1 cut(s) 177
Fnu4HI GCNGC 2 cut(s) 269, 549
FokI GGATG 5 cut(s) 286, 350, 539, 635, 827
FriOI GRGCYC 1 cut(s) 520
Fsp4HI GCNGC 2 cut(s) 269, 549
FspBI CTAG 2 cut(s) 552, 940
GluI GCNGC 2 cut(s) 269, 549
GsaI CCCAGC 1 cut(s) 57
GsuI CTGGAG 1 cut(s) 468
HaeIII GGCC 1 cut(s) 563
Hin1II CATG 5 cut(s) 265, 329, 350, 440, 630
HincII GTYRAC 2 cut(s) 640, 882
HindII GTYRAC 2 cut(s) 640, 882
HinfI GANTC 2 cut(s) 39, 782
HphI GGTGA 3 cut(s) 150, 692, 830
Hpy166II GTNNAC 4 cut(s) 38, 430, 640, 882
Hpy188I TCNGA 2 cut(s) 768, 862
Hpy188III TCNNGA 5 cut(s) 180, 347, 437, 620, 930
Hpy8I GTNNAC 4 cut(s) 38, 430, 640, 882
HpyAV CCTTC 4 cut(s) 165, 199, 278, 747
HpyCH4III ACNGT 4 cut(s) 35, 487, 581, 832
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 906
HpyF3I CTNAG 5 cut(s) 65, 200, 588, 597, 618
Hsp92II CATG 5 cut(s) 265, 329, 350, 440, 630
Kzo9I GATC 3 cut(s) 454, 601, 763
LmnI GCTCC 2 cut(s) 148, 759
Lsp1109I GCAGC 2 cut(s) 255, 560
LweI GCATC 7 cut(s) 202, 255, 385, 406, 433, 517, 613
MaeI CTAG 2 cut(s) 552, 940
MaeIII GTNAC 3 cut(s) 130, 611, 666
MalI GATC 3 cut(s) 456, 603, 765
MboI GATC 3 cut(s) 454, 601, 763
MboII GAAGA 9 cut(s) 95, 125, 128, 131, 134, 137, 422, 425, 545
MfeI CAATTG 3 cut(s) 140, 570, 723
MhlI GDGCHC 2 cut(s) 432, 520
MluCI AATT 5 cut(s) 20, 140, 570, 723, 874
MlyI GAGTC 2 cut(s) 33, 791
MmeI TCCRAC 1 cut(s) 670
Mph1103I ATGCAT 2 cut(s) 421, 628
MseI TTAA 1 cut(s) 99
MslI CAYNNNNRTG 3 cut(s) 330, 435, 441
MunI CAATTG 3 cut(s) 140, 570, 723
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 2 cut(s) 221, 906
NdeII GATC 3 cut(s) 454, 601, 763
NlaIII CATG 5 cut(s) 265, 329, 350, 440, 630
NlaIV GGNNCC 2 cut(s) 27, 150
NsiI ATGCAT 2 cut(s) 421, 628
PagI TCATGA 2 cut(s) 346, 436
PkrI GCNGC 2 cut(s) 270, 550
PleI GAGTC 2 cut(s) 33, 790
PpsI GAGTC 2 cut(s) 33, 790
PspFI CCCAGC 1 cut(s) 53
PspN4I GGNNCC 2 cut(s) 27, 150
PspPI GGNCC 2 cut(s) 25, 562
PstI CTGCAG 1 cut(s) 525
RsaI GTAC 1 cut(s) 834
RsaNI GTAC 1 cut(s) 833
RseI CAYNNNNRTG 3 cut(s) 330, 435, 441
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 2 cut(s) 269, 549
Sau3AI GATC 3 cut(s) 454, 601, 763
Sau96I GGNCC 2 cut(s) 25, 562
SbfI CCTGCAGG 1 cut(s) 525
ScaI AGTACT 1 cut(s) 834
SchI GAGTC 2 cut(s) 33, 791
SdaI CCTGCAGG 1 cut(s) 525
SduI GDGCHC 2 cut(s) 432, 520
SfaNI GCATC 7 cut(s) 202, 255, 385, 406, 433, 517, 613
SfcI CTRYAG 1 cut(s) 521
SinI GGWCC 1 cut(s) 25
SmiMI CAYNNNNRTG 3 cut(s) 330, 435, 441
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
Sse8387I CCTGCAGG 1 cut(s) 525
Sse9I AATT 5 cut(s) 20, 140, 570, 723, 874
SsiI CCGC 4 cut(s) 108, 146, 170, 271
SspMI CTAG 2 cut(s) 552, 940
TaaI ACNGT 4 cut(s) 35, 487, 581, 832
TaqI TCGA 2 cut(s) 210, 232
TasI AATT 5 cut(s) 20, 140, 570, 723, 874
TatI WGTACW 1 cut(s) 832
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 3 cut(s) 472, 601, 916
TseI GCWGC 2 cut(s) 268, 548
TspDTI ATGAA 5 cut(s) 290, 297, 335, 423, 720
TspGWI ACGGA 1 cut(s) 375
TspRI CASTG 3 cut(s) 472, 601, 916
VneI GTGCAC 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 25
XspI CTAG 2 cut(s) 552, 940
ZrmI AGTACT 1 cut(s) 834
Zsp2I ATGCAT 2 cut(s) 421, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.