MD15G1141500.v1.1

E3 ubiquitin ligase BIG

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010233
Physical Location & Seq
Reverse (-)
10433769 .. 10438575
4807 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1141500.v1.1.491

Sequence Viewer

Length: 963 bp
ATGGAGAATGATGACGTCAAATTGGGGTCTGCCGGCGGACTCAAATCCAACAACAACAACAGCGTCGTCGAACAAGAACAAAACCCCTTGAGAGAATGCGAAGAAGGAGAAGGAGAAGAAGAAGTAAGCAGTGAGCCTTCGCAGGAAGAGGAGGTGGATCCTCCCTCCTCACCTCCTGTTCCTCAACCTTCAAGAACTCCCTTCAGTAATCTCAGTCAGGTCGATGCTGACCTAGCCCTTGCTCGAACCCTCCAAGAACAGGAAAGGGCATACATGATGCTACGAATGAACAATGTAGACAGTGATTATGGAAGTTGGGAAGCTGGAAGTTATGTGCATGAGGATGAGGATGATTTCAATGAATTTGACGACGATACTGATGATAATGATGACGAGGAACAAGATGATGAAACTGAGGTTGACAATGATGAAGATGCATTTGATGTGCATGCTCATGATGATGCTGGAGAGAATGACAACCCCAGTGTCGAATATGATCCGGCTGTTTTTTCAAGCGATGAGGCCTATGCAAGAGCCCTGCAGGATGCTGAAGATAGAGAGATGGCTGCTAGACTGATGGCACTTGCTCGGATTAATGATCGCGAAGTTGAGGACATAGAGGAACATGGTGGTAACTCTCAGGATACATGGGATGAGGTTGACCCTGATGAACTTTCATATGAGGAGTTACTTGCATTGGCTGAAGTTGTTGGAACTGAGAGCAGAGGGCTTTCAGCTGATAATATCGCCTCGTTGCCTTCAGTAAGCTACAAGACAGGAAGTGGTCAGAATGGGAGCAATGAGTCGTGTGTCATTTGTCGGTTGGACTACGAGGATGGTGAAAACTTGACATTACTATCTTGCAAACATTCCTATCATTCTGAGTGCATAAACAATTGGTTGACAATTAACAAGGTCTGCCCTGTTTGCAGTGCTGAAGTTTCCACATCTGGAAACAGCTAG

Protein Analysis

321

Amino Acids

35.36

Weight (kDa)

4.05

Isoelectric Point (pI)

54.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 265 - 310 1.1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 269 - 310 8.3e-13 Ring finger domain
zf-C3HC4_2 PF13923 270 - 310 6.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 270 - 297 1.3e-07 RING-like zinc finger
zf-C3HC4 PF00097 270 - 310 7.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 270 - 311 8.5e-07 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 18
AccI GTMKAC 1 cut(s) 297
AccII CGCG 1 cut(s) 603
AciI CCGC 1 cut(s) 36
AclWI GGATC 3 cut(s) 152, 165, 491
AcsI RAATTY 1 cut(s) 362
AcuI CTGAAG 5 cut(s) 187, 570, 723, 744, 957
AcyI GRCGYC 1 cut(s) 15
AfiI CCNNNNNNNGG 1 cut(s) 259
AgsI TTSAA 3 cut(s) 192, 358, 513
AluBI AGCT 4 cut(s) 323, 737, 768, 960
AluI AGCT 4 cut(s) 323, 737, 768, 960
AlwI GGATC 3 cut(s) 152, 165, 491
AoxI GGCC 1 cut(s) 522
ApeKI GCWGC 1 cut(s) 566
ApoI RAATTY 1 cut(s) 362
AseI ATTAAT 1 cut(s) 594
AsuHPI GGTGA 2 cut(s) 162, 851
BamHI GGATCC 1 cut(s) 157
BanII GRGCYC 1 cut(s) 538
BbvI GCAGC 1 cut(s) 553
BccI CCATC 3 cut(s) 556, 571, 830
BciVI GTATCC 1 cut(s) 637
BfaI CTAG 3 cut(s) 233, 570, 961
BfmI CTRYAG 1 cut(s) 539
BfuI GTATCC 1 cut(s) 637
BisI GCNGC 1 cut(s) 567
BlsI GCNGC 1 cut(s) 568
BmiI GGNNCC 1 cut(s) 159
BmrI ACTGGG 1 cut(s) 477
BmsI GCATC 5 cut(s) 214, 267, 424, 451, 535
BmuI ACTGGG 1 cut(s) 477
BpmI CTGGAG 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 109
BsaHI GRCGYC 1 cut(s) 15
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 483
Bse3DI GCAATG 1 cut(s) 805
BseGI GGATG 5 cut(s) 349, 355, 550, 658, 841
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMI GCAATG 1 cut(s) 805
BseMII CTCAG 5 cut(s) 226, 405, 653, 708, 873
BseNI ACTGG 1 cut(s) 483
BseRI GAGGAG 3 cut(s) 157, 164, 698
BseXI GCAGC 1 cut(s) 553
Bsh1236I CGCG 1 cut(s) 603
BshFI GGCC 1 cut(s) 524
BsiSI CCGG 2 cut(s) 33, 500
BslI CCNNNNNNNGG 1 cut(s) 259
BsmI GAATGC 1 cut(s) 101
BsnI GGCC 1 cut(s) 524
Bsp1286I GDGCHC 1 cut(s) 538
Bsp143I GATC 3 cut(s) 157, 496, 598
Bsp68I TCGCGA 1 cut(s) 603
BspACI CCGC 1 cut(s) 36
BspANI GGCC 1 cut(s) 524
BspCNI CTCAG 5 cut(s) 225, 406, 652, 709, 874
BspFNI CGCG 1 cut(s) 603
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 1 cut(s) 159
BspMAI CTGCAG 1 cut(s) 543
BspPI GGATC 3 cut(s) 152, 165, 491
BsrDI GCAATG 1 cut(s) 805
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 1 cut(s) 483
BssAI RCCGGY 1 cut(s) 32
BssMI GATC 3 cut(s) 157, 496, 598
BssNI GRCGYC 1 cut(s) 15
Bst4CI ACNGT 1 cut(s) 302
Bst6I CTCTTC 1 cut(s) 141
BstACI GRCGYC 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 34, 450
BstDEI CTNAG 5 cut(s) 212, 414, 639, 717, 882
BstF5I GGATG 5 cut(s) 349, 355, 550, 658, 841
BstFNI CGCG 1 cut(s) 603
BstKTI GATC 3 cut(s) 160, 499, 601
BstMBI GATC 3 cut(s) 157, 496, 598
BstMWI GCNNNNNNNGC 2 cut(s) 233, 927
BstNSI RCATGY 1 cut(s) 452
BstSFI CTRYAG 1 cut(s) 539
BstUI CGCG 1 cut(s) 603
BstV1I GCAGC 1 cut(s) 553
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BsuI GTATCC 1 cut(s) 637
BsuRI GGCC 1 cut(s) 524
BtgZI GCGATG 1 cut(s) 531
BtsCI GGATG 5 cut(s) 349, 355, 550, 658, 841
BtsI GCAGTG 2 cut(s) 136, 937
BtsIMutI CAGTG 4 cut(s) 136, 307, 490, 937
BtuMI TCGCGA 1 cut(s) 603
Cac8I GCNNGC 2 cut(s) 34, 450
CciI TCATGA 1 cut(s) 454
Cfr10I RCCGGY 1 cut(s) 32
CseI GACGC 1 cut(s) 52
CviAII CATG 6 cut(s) 274, 338, 449, 455, 626, 648
DdeI CTNAG 5 cut(s) 212, 414, 639, 717, 882
DpnI GATC 3 cut(s) 159, 498, 600
DpnII GATC 3 cut(s) 157, 496, 598
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
EciI GGCGGA 1 cut(s) 51
Eco147I AGGCCT 1 cut(s) 524
Eco24I GRGCYC 1 cut(s) 538
Eco57I CTGAAG 5 cut(s) 187, 570, 723, 744, 957
EcoT22I ATGCAT 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 538
FaeI CATG 6 cut(s) 277, 341, 452, 458, 629, 651
FatI CATG 6 cut(s) 273, 337, 448, 454, 625, 647
FauNDI CATATG 1 cut(s) 679
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 1 cut(s) 567
FokI GGATG 5 cut(s) 356, 362, 557, 665, 848
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 1 cut(s) 567
FspBI CTAG 3 cut(s) 233, 570, 961
GluI GCNGC 1 cut(s) 567
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 1 cut(s) 524
HapII CCGG 2 cut(s) 33, 500
HgaI GACGC 1 cut(s) 52
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 6 cut(s) 277, 341, 452, 458, 629, 651
HincII GTYRAC 3 cut(s) 421, 661, 903
HindII GTYRAC 3 cut(s) 421, 661, 903
HinfI GANTC 2 cut(s) 39, 803
HpaII CCGG 2 cut(s) 33, 500
HphI GGTGA 2 cut(s) 162, 851
Hpy166II GTNNAC 4 cut(s) 298, 421, 661, 903
Hpy188I TCNGA 3 cut(s) 591, 789, 883
Hpy188III TCNNGA 5 cut(s) 192, 455, 602, 641, 951
Hpy8I GTNNAC 4 cut(s) 298, 421, 661, 903
Hpy99I CGWCG 3 cut(s) 68, 71, 374
HpyAV CCTTC 6 cut(s) 98, 104, 147, 198, 211, 768
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 9 cut(s) 337, 437, 448, 530, 541, 695, 864, 888, 930
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 927
HpyF3I CTNAG 5 cut(s) 212, 414, 639, 717, 882
HpySE526I ACGT 1 cut(s) 15
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 6 cut(s) 277, 341, 452, 458, 629, 651
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Kzo9I GATC 3 cut(s) 157, 496, 598
LmnI GCTCC 1 cut(s) 795
Lsp1109I GCAGC 1 cut(s) 553
LweI GCATC 5 cut(s) 214, 267, 424, 451, 535
MaeI CTAG 3 cut(s) 233, 570, 961
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 2 cut(s) 632, 687
MalI GATC 3 cut(s) 159, 498, 600
MboI GATC 3 cut(s) 157, 496, 598
MboII GAAGA 6 cut(s) 113, 128, 131, 158, 443, 563
MfeI CAATTG 1 cut(s) 895
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 1 cut(s) 538
MluCI AATT 4 cut(s) 20, 362, 895, 906
MlyI GAGTC 2 cut(s) 33, 812
MmeI TCCRAC 3 cut(s) 72, 691, 804
Mph1103I ATGCAT 1 cut(s) 439
MroNI GCCGGC 1 cut(s) 32
MseI TTAA 2 cut(s) 594, 909
MslI CAYNNNNRTG 3 cut(s) 342, 453, 459
MspA1I CMGCKG 1 cut(s) 737
MspI CCGG 2 cut(s) 33, 500
MunI CAATTG 1 cut(s) 895
Mva1269I GAATGC 1 cut(s) 101
MvnI CGCG 1 cut(s) 603
MwoI GCNNNNNNNGC 2 cut(s) 233, 927
NaeI GCCGGC 1 cut(s) 34
NdeI CATATG 1 cut(s) 679
NdeII GATC 3 cut(s) 157, 496, 598
NgoMIV GCCGGC 1 cut(s) 32
NlaIII CATG 6 cut(s) 277, 341, 452, 458, 629, 651
NlaIV GGNNCC 1 cut(s) 159
NruI TCGCGA 1 cut(s) 603
NsiI ATGCAT 1 cut(s) 439
NspI RCATGY 1 cut(s) 452
PaeI GCATGC 1 cut(s) 452
PagI TCATGA 1 cut(s) 454
PceI AGGCCT 1 cut(s) 524
PctI GAATGC 1 cut(s) 101
PdiI GCCGGC 1 cut(s) 34
PkrI GCNGC 1 cut(s) 568
PleI GAGTC 2 cut(s) 33, 811
PpsI GAGTC 2 cut(s) 33, 811
PshBI ATTAAT 1 cut(s) 594
PspN4I GGNNCC 1 cut(s) 159
PstI CTGCAG 1 cut(s) 543
PsuI RGATCY 1 cut(s) 157
PvuII CAGCTG 1 cut(s) 737
RruI TCGCGA 1 cut(s) 603
RseI CAYNNNNRTG 3 cut(s) 342, 453, 459
SaqAI TTAA 2 cut(s) 594, 909
SatI GCNGC 1 cut(s) 567
Sau3AI GATC 3 cut(s) 157, 496, 598
SbfI CCTGCAGG 1 cut(s) 543
SchI GAGTC 2 cut(s) 33, 812
SdaI CCTGCAGG 1 cut(s) 543
SduI GDGCHC 1 cut(s) 538
SfaNI GCATC 5 cut(s) 214, 267, 424, 451, 535
SfcI CTRYAG 1 cut(s) 539
SmiMI CAYNNNNRTG 3 cut(s) 342, 453, 459
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
SphI GCATGC 1 cut(s) 452
Sse8387I CCTGCAGG 1 cut(s) 543
Sse9I AATT 4 cut(s) 20, 362, 895, 906
SseBI AGGCCT 1 cut(s) 524
SsiI CCGC 1 cut(s) 36
SspMI CTAG 3 cut(s) 233, 570, 961
StuI AGGCCT 1 cut(s) 524
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 1 cut(s) 18
TaqI TCGA 4 cut(s) 69, 222, 244, 489
TasI AATT 4 cut(s) 20, 362, 895, 906
Tru1I TTAA 2 cut(s) 594, 909
Tru9I TTAA 2 cut(s) 594, 909
TscAI CASTG 4 cut(s) 136, 307, 490, 937
TseI GCWGC 1 cut(s) 566
TspDTI ATGAA 6 cut(s) 302, 375, 423, 444, 666, 684
TspRI CASTG 4 cut(s) 136, 307, 490, 937
VspI ATTAAT 1 cut(s) 594
XapI RAATTY 1 cut(s) 362
XceI RCATGY 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 297
XspI CTAG 3 cut(s) 233, 570, 961
ZraI GACGTC 1 cut(s) 16
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.