FvH4_1g01730

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004627
Physical Location & Seq
Reverse (-)
912603 .. 918787
6185 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g01730.t4

Sequence Viewer

Length: 4212 bp
ATGACGTTTGATCAAAATTCGGTTCCCCAAGATTTGAGGCCTTTAAATGTAGCGAGGTCTGTGGCTGAGGAGCCCCCCCGCATTGCATTGGCTACTTCAGCTGGGTTTATACCCAATTTGGCTCAAGGTAGTAGTCCTATGTATTACCCTGGGCCGGTTTCGGAGGCGGGGTATGTTGGTTTAGGGTATGGGAATCCGGTGTCCACATGGGGGCCGAGGATGCCTGTGACGGTGGGGCATCAGGGTGTGAGCCCTGCGATGGGAGTTGGAATCGGTTACAGTCCTAATTTGGGGAGTAGGGTTGTTGGGAATGCTGTTGATCTTGTGAGCTCTCCTGCTACAGCTGGAGCTGGGCCTTATGTGTGTAGTTCGAATATGGGGAATATGGTTGTTGGTAATGGTATGGATCAGGGTGTTAGTGATATGTCAGCTCGGTTTGGTTATAGTACCAATTTAGGCAGTAAGGGCAATATTGCTGATCAGTCAGGTAGTGATTCGATGTCTGGGTATGCTTATAGTCCTAATATGGGAAATCGGAGTGATCAAGCCAGTGATGATGGTGGAGATGATTCTGTGTCAGGGAAGAAAATAAAGCTGCTGTGCAGTTTTGGAGGCAAGATTTTGCCCAGACCAAGTGATGGGATGTTGAGATATGTTGGAGGACATACAAGGATTATTAGTGTTCGGAGAGATGTGAACCTGAACGAGCTAGTGCAGAAAATGGTGGATTCGTATGGGCAATCTGTGGTTATCAAGTATCAGCTACCCGACGAGGATCTTGATGCACTAGTGTCAGTTTCATGTGCTGATGATCTTGACAATATGAAGGATGAGTATGAGAAGTTGGTGGAGAAATCTCCAGATGGGTCAGCAAAATTGAGGGTATTTCTTTTTTCTGCTTCAGACCTTGATACATATGGTTCTGTAAAGTATGGGGATTTAAATGAGAGTGGCCAGAGATATGTTGATGCTGTGAATGGGATTTCGGATGGGGTTGGTAGTGGTATCATGAGGAGGGAGAGTATAACAAGTGCAACTTCAACTCAAAATTCTGATTATAGTGGAATTGATGTTGTTGATAGTTCCGTTCCTAGCCAGGGGGACAAGTTATCCCCTAAAGGAGATTTGACAAACTCTCAGGACACTGGTACAAGATTGGGTTTTCCAGATCCAAATCCTGCAGTGGTTTCTACTATTCCATCAGGCATTAATGTGGTTAAGACTGGGCCTCCCCAAACATCATATTCCCCCCCTGAGGTTGAGTTTGAAAGGACTGCTCCTGTTACAGTATCACAGCAGCAACTGGGGTTGCAGCAACCTGGAATTGACATTCAGTCAACTTCTCCATATCTGCAAGCTTATGTTAGCCCTCACCAGCTTCCTCCCCATATGGGATTCCCAAACAATCACCTGTTTGGGACTGCTGGTCCTGTCTATACCCAACAACAATTCCGTGATAGTGGTGCTGGCATGACCTCACATCATTTTGTTCCTGCTGTGCATATGACAATGAGTCCATCTTCTCATGTTAATATGAGACCAAATGTACTTCAGCCATTGATGCAACCCCAACAGACTCGATTGGATCATCATTATGTGGATGAAAGTGCCTTTTGTCCCAGAGTTGTCCAGCTTCCCGCTGAACAAAGCTATAATACATATCAGGTTCAGGTCCCCTCACCAGCACCAGGAGGTGCTTATGGTTGGCATCAGATTACACCACAGGAGCATGTGATGTATCGTGATGGTTTGGTATCGCACCAACAGGTAATGTATCCTGAGAAATCCAACAGGTTGGAAGACTGCTTTATGTGTCAGAAGGCTTTGCCTCATGCACATTCTGACCCGGTACCAGTACAGGGTCAGCGCGATGGTGGTGCGAGTCCTGTATCTGATTCAGATTCAAATTACCATGGTCTTCTTCTTGAGGAAAACTGGAGACCTCAGCCAACAAACAGGGTGTTGGTAAGTGGAGCATTGGGGGAAGGTAATTTAGGGCAGGGAGTTGATATTCGACCCAGAGTCCAAGGCCTGGTAGATCCTCTTGGAACATCACATTCAGATGGCGCAGGGATAGTGCAGAATTCTTCTGAGTGCATATATGAGGAGAGAAATTATCTTCATCAAGCGGATCATCCAATGATATCAGCTCCCCAGGGCGTCATGAGGAGGGCTGCCCCAGCCCAGTATCATGTTAAACATGAAGATTTGGTTAACACTCCAGTTAATCAAGAAGTTCCACTTACTGGAGCCCTGAATGTTCAAACTTCAGACCTGGTTCGCGAGTCTCCGCAAGTATACTTTAATAAATTTCCAAAAGAAGAGATGATGGAAACAATCAGGATAAGCCCGTCAGAGGTTTATGTTAAAAATGAGCATGCTAAGTCACCTATTGAAACACCTAGAAGGGAAGACAGCTATGATCAACAACCACCACAACTTGCAGGAAGGGAGTTACCTGTAGAGAATACCTTAGACAAGAATCAGTTTGTTCCAACTGAAGTACTAGCTTCTTACACTACTGGAGCCCCACAAGTCCATACTACACAACCAGTAGAAATCAATGAAACACCACAACCACCTGTGTGGGGAAATCCTGAATCTTATCAGCAATCAAGAATTGGATTCCCTGTGGATTCTCATGCATTCCATTATGGAAACCCTGTTGTGTCCACCCCTCTACCTACCAGAACTCAACCTCCTGCTGAAAGGAGAAACGAGAACTTGAGATTGCATCCCAACATGGTACATACTGATGTTGAAGTTGTTACCTTGAATGGTAACATGCCATCTTCTTTCTCTCCACCTATGATAACAGTTGATGTCCAAGACTCTTCAAACTCACTATTCAGCGATCAGGATCCTTGGAATTTGCGCCATGACACTCATCTTCCGCCTCCTATACCTACCAAAATACAATTGAAAAAGGAACCCTGTACAAATAGAGATCTCTTTGGTGGCAATGGTGCAGAACTAAATACAGAAATGCAATTGGAGGATGGAGGTCAGCCACTGGGTAATTTGAACAAGGATCTTAATCCAGATCATGCCCAGTCTTCCAAAGGATCAGCAGAGGAACAGATTAAGCAAGACCTTCAAGCTGTTGCTGAGGGTGTAGCTGCCTCAGTTTTTAAGTCATCTTCACTGTCAAATCCGGTTTTTAATGACAAAAATGAGTCTGCATATGATTCCAACCAAGATGAAGGTCAAATCAGCGTTTCAAGTATGCAGCATAGCGTTGAGGACGTCAAGACCAAATGTCCAGAGAAGCCAAATTTGGGGTTTCCAGTATCAGATGGCATCGGCCGGCTGCAGATTATTAAGAATGATGACCTTGAAGAGCTCAGAGAATTAGGTTCTGGAACATTTGGTACAGTCTATCATGGAAAGTGGAGGGGCACTGATGTTGCAATCAAACGAATCAACGATAGGTGTTTTGCTGGGAAGCCTTCAGAACAGGAGCGCATGAGAAACGATTTCTGGAATGAGGCCATCAATCTGGCTGACTTGCACCACCCAAATGTGGTAGCTTTTTATGGTGTTGTACTTGATGGCCCAGGAGGTTCTGTGGCAACCGTGACAGAGTTTATGGTTAATGGTTCCCTTAGAAATGCGTTGCAGAAGAATGAGAAGAGTCTGGACAAGCGCAAACGCCTATTGATTGCAATGGATGTGGCATTTGGAATGGAGTACCTGCATGGAAAGAATATAGTGCACTTTGATTTGAAAAGTGACAATTTACTGGTCAATCTTCGAGATCCACACCGCCCGATTTGCAAGGTTGGTGATTTGGGCCTATCAAAGGTGAAATGTCAGACACTGATCTCTGGTGGCGTGCGAGGAACACTTCCTTGGATGGCACCTGAACTTTTGAATGGTAGTAGTAGCCTTGTCTCTGAGAAGGTTGATGTGTTTTCTTTTGGTATCGTACTGTGGGAGCTCCTCACTGGAGAAGAACCATACGGGGACTTGCACTATGGGGCTATCATAGGCGGTATTGTGAGCAATACATTGCGGCCACCAATCCCTGAGTCTTGTGATCCAGAGTGGAAATCATTGATGGAGAGATGCTGGTCAGCAGAGCCATCTGAGAGACTGAACTTCACTGAGATTGCAAACCAATTACGTGCTATGGCTGCAAAGATTCCTCCTAAAGTACAAGGCCAAGCACAGCAGCAACCGCCCTCGACGCAGCCTCAGGTTCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1404

Amino Acids

152.8

Weight (kDa)

5.25

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 216 - 299 1e-14 PB1 domain
Pkinase PF00069 1113 - 1264 5.8e-23 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1114 - 1264 2.7e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 3249
Acc36I ACCTGC 1 cut(s) 3702
Acc65I GGTACC 1 cut(s) 1849
AccB1I GGYRCC 2 cut(s) 1849, 3859
AccB7I CCANNNNNTGG 3 cut(s) 638, 2032, 2246
AccI GTMKAC 1 cut(s) 2298
AccII CGCG 2 cut(s) 1869, 2283
AcoI YGGCCR 3 cut(s) 952, 3304, 4016
AcsI RAATTY 6 cut(s) 16, 1048, 2083, 2309, 2869, 3274
AcuI CTGAAG 6 cut(s) 81, 885, 1535, 2253, 2520, 3435
AcyI GRCGYC 2 cut(s) 2160, 3246
AhdI GACNNNNNGTC 5 cut(s) 1333, 1425, 1512, 2021, 3258
AhlI ACTAGT 1 cut(s) 787
AjnI CCWGG 8 cut(s) 148, 1095, 1318, 1687, 2031, 2154, 2274, 3556
AjuI GAANNNNNNNTTGG 4 cut(s) 3260, 3292, 3835, 3867
AleI CACNNNNGTG 1 cut(s) 2584
AloI GAACNNNNNNTCC 2 cut(s) 2683, 2715
Alw21I GWGCWC 4 cut(s) 332, 3345, 3717, 3942
Alw26I GTCTC 5 cut(s) 1531, 1933, 2292, 3898, 4087
Alw44I GTGCAC 1 cut(s) 3713
AlwNI CAGNNNCTG 3 cut(s) 1303, 3013, 3820
ApaLI GTGCAC 1 cut(s) 3713
ApoI RAATTY 6 cut(s) 16, 1048, 2083, 2309, 2869, 3274
AseI ATTAAT 1 cut(s) 1209
Asp718I GGTACC 1 cut(s) 1849
AspLEI GCGC 5 cut(s) 1869, 2069, 2877, 3465, 3648
AspS9I GGNCC 8 cut(s) 152, 212, 353, 1226, 1427, 1672, 3554, 3793
AsuC2I CCSGG 1 cut(s) 1847
AsuHPI GGTGA 6 cut(s) 1364, 1400, 1671, 2379, 3797, 3817
AsuII TTCGAA 1 cut(s) 371
AvaII GGWCC 2 cut(s) 1427, 1672
AxyI CCTNAGG 2 cut(s) 1254, 4197
BaeGI GKGCMC 2 cut(s) 3401, 3717
BalI TGGCCA 1 cut(s) 954
BamHI GGATCC 1 cut(s) 2860
BanI GGYRCC 2 cut(s) 1849, 3859
BanII GRGCYC 7 cut(s) 75, 254, 332, 2254, 2530, 3345, 3942
BbsI GAAGAC 4 cut(s) 1806, 1910, 2418, 3048
Bbv12I GWGCWC 4 cut(s) 332, 3345, 3717, 3942
BbvCI CCTCAGC 3 cut(s) 66, 1944, 3108
BcgI CGANNNNNNTGC 2 cut(s) 1859, 1893
BciT130I CCWGG 8 cut(s) 150, 1097, 1320, 1689, 2033, 2156, 2276, 3558
BciVI GTATCC 1 cut(s) 1785
BclI TGATCA 4 cut(s) 10, 478, 541, 2422
BcnI CCSGG 1 cut(s) 1847
BcoDI GTCTC 5 cut(s) 1531, 1933, 2292, 3898, 4087
BcuI ACTAGT 1 cut(s) 787
BfaI CTAG 5 cut(s) 710, 788, 1092, 2403, 2507
BfmI CTRYAG 4 cut(s) 339, 1179, 2460, 3311
BfuAI ACCTGC 1 cut(s) 3702
BfuI GTATCC 1 cut(s) 1785
BglII AGATCT 1 cut(s) 2947
BmcAI AGTACT 1 cut(s) 2505
Bme1390I CCNGG 9 cut(s) 150, 1097, 1320, 1689, 1847, 2033, 2156, 2276, 3558
Bme18I GGWCC 2 cut(s) 1427, 1672
BmeRI GACNNNNNGTC 5 cut(s) 1333, 1425, 1512, 2021, 3258
BmgT120I GGNCC 8 cut(s) 152, 212, 353, 1226, 1427, 1672, 3554, 3793
BmrFI CCNGG 9 cut(s) 150, 1097, 1320, 1689, 1847, 2033, 2156, 2276, 3558
BmrI ACTGGG 5 cut(s) 1233, 1313, 2179, 3023, 3046
BmsI GCATC 9 cut(s) 210, 247, 772, 958, 1551, 1717, 2743, 3309, 4058
BmuI ACTGGG 5 cut(s) 1233, 1313, 2179, 3023, 3046
BpiI GAAGAC 4 cut(s) 1806, 1910, 2418, 3048
BpmI CTGGAG 7 cut(s) 366, 843, 1957, 2205, 2268, 2544, 3969
Bpu10I CCTNAGC 3 cut(s) 66, 1944, 3108
Bpu14I TTCGAA 1 cut(s) 371
BpuEI CTTGAG 3 cut(s) 108, 1946, 2746
BpuMI CCSGG 1 cut(s) 1847
BsaAI YACGTR 1 cut(s) 4127
BsaBI GATNNNNATC 3 cut(s) 1173, 2859, 3036
BsaHI GRCGYC 2 cut(s) 2160, 3246
BsaI GGTCTC 2 cut(s) 1531, 1933
BsaWI WCCGGW 2 cut(s) 196, 3154
Bse118I RCCGGY 2 cut(s) 154, 3306
Bse21I CCTNAGG 2 cut(s) 1254, 4197
Bse3DI GCAATG 4 cut(s) 81, 2968, 3672, 4010
Bse8I GATNNNNATC 3 cut(s) 1173, 2859, 3036
BseBI CCWGG 8 cut(s) 150, 1097, 1320, 1689, 2033, 2156, 2276, 3558
BseJI GATNNNNATC 3 cut(s) 1173, 2859, 3036
BseMI GCAATG 4 cut(s) 81, 2968, 3672, 4010
BseRI GAGGAG 5 cut(s) 83, 1027, 2120, 2182, 3932
BseSI GKGCMC 2 cut(s) 3401, 3717
BseX3I CGGCCG 1 cut(s) 3304
BseYI CCCAGC 4 cut(s) 101, 350, 2179, 3440
BsgI GTGCAG 4 cut(s) 622, 734, 2099, 2988
Bsh1236I CGCG 2 cut(s) 1869, 2283
Bsh1285I CGRYCG 1 cut(s) 3307
BshNI GGYRCC 2 cut(s) 1849, 3859
BsiEI CGRYCG 1 cut(s) 3307
BsiHKAI GWGCWC 4 cut(s) 332, 3345, 3717, 3942
BsiSI CCGG 5 cut(s) 155, 197, 1847, 3155, 3307
BslFI GGGAC 5 cut(s) 1115, 1432, 1602, 1658, 3980
BsmAI GTCTC 5 cut(s) 1531, 1933, 2292, 3898, 4087
BsmFI GGGAC 5 cut(s) 1115, 1432, 1602, 1658, 3980
BsmI GAATGC 2 cut(s) 316, 2645
Bso31I GGTCTC 2 cut(s) 1531, 1933
Bsp119I TTCGAA 1 cut(s) 371
Bsp1286I GDGCHC 9 cut(s) 75, 254, 332, 2254, 2530, 3345, 3401, 3717, 3942
Bsp1407I TGTACA 1 cut(s) 2936
Bsp19I CCATGG 1 cut(s) 1912
Bsp68I TCGCGA 1 cut(s) 2283
BspFNI CGCG 2 cut(s) 1869, 2283
BspHI TCATGA 2 cut(s) 1008, 2163
BspMAI CTGCAG 2 cut(s) 1183, 3315
BspMI ACCTGC 1 cut(s) 3702
BspQI GCTCTTC 1 cut(s) 3333
BspT104I TTCGAA 1 cut(s) 371
BspT107I GGYRCC 2 cut(s) 1849, 3859
BspTNI GGTCTC 2 cut(s) 1531, 1933
BsrDI GCAATG 4 cut(s) 81, 2968, 3672, 4010
BsrFI RCCGGY 2 cut(s) 154, 3306
BsrGI TGTACA 1 cut(s) 2936
BssAI RCCGGY 2 cut(s) 154, 3306
BssNAI GTATAC 1 cut(s) 2299
BssNI GRCGYC 2 cut(s) 2160, 3246
BssT1I CCWWGG 4 cut(s) 1912, 2026, 2864, 3851
Bst1107I GTATAC 1 cut(s) 2299
Bst2UI CCWGG 8 cut(s) 150, 1097, 1320, 1689, 2033, 2156, 2276, 3558
Bst4CI ACNGT 8 cut(s) 232, 281, 1288, 2818, 3147, 3376, 3577, 3933
Bst6I CTCTTC 4 cut(s) 2316, 2839, 3333, 3626
BstACI GRCGYC 2 cut(s) 2160, 3246
BstAUI TGTACA 1 cut(s) 2936
BstBAI YACGTR 1 cut(s) 4127
BstBI TTCGAA 1 cut(s) 371
BstC8I GCNNGC 5 cut(s) 1356, 1468, 2379, 3308, 3836
BstDSI CCRYGG 1 cut(s) 1912
BstENI CCTNNNNNAGG 1 cut(s) 3800
BstFNI CGCG 2 cut(s) 1869, 2283
BstHHI GCGC 5 cut(s) 1869, 2069, 2877, 3465, 3648
BstMAI GTCTC 5 cut(s) 1531, 1933, 2292, 3898, 4087
BstMCI CGRYCG 1 cut(s) 3307
BstMWI GCNNNNNNNGC 9 cut(s) 98, 220, 465, 1561, 2180, 3774, 4136, 4180, 4189
BstNI CCWGG 8 cut(s) 150, 1097, 1320, 1689, 2033, 2156, 2276, 3558
BstNSI RCATGY 3 cut(s) 1733, 2381, 2788
BstSCI CCNGG 9 cut(s) 148, 1095, 1318, 1687, 1845, 2031, 2154, 2274, 3556
BstSFI CTRYAG 4 cut(s) 339, 1179, 2460, 3311
BstSLI GKGCMC 2 cut(s) 3401, 3717
BstUI CGCG 2 cut(s) 1869, 2283
BstV2I GAAGAC 4 cut(s) 1806, 1910, 2418, 3048
BstX2I RGATCY 7 cut(s) 775, 1168, 2038, 2860, 2947, 3031, 3757
BstXI CCANNNNNNTGG 3 cut(s) 1795, 2586, 3499
BstYI RGATCY 7 cut(s) 775, 1168, 2038, 2860, 2947, 3031, 3757
BstZ17I GTATAC 1 cut(s) 2299
BstZI CGGCCG 1 cut(s) 3304
Bsu36I CCTNAGG 2 cut(s) 1254, 4197
BsuI GTATCC 1 cut(s) 1785
BtgI CCRYGG 1 cut(s) 1912
BtgZI GCGATG 2 cut(s) 272, 1884
BtsI GCAGTG 1 cut(s) 1188
BtsIMutI CAGTG 9 cut(s) 556, 1143, 1188, 3011, 3143, 3399, 3818, 3945, 4104
BtuMI TCGCGA 1 cut(s) 2283
BveI ACCTGC 1 cut(s) 3702
Cac8I GCNNGC 5 cut(s) 1356, 1468, 2379, 3308, 3836
CaiI CAGNNNCTG 3 cut(s) 1303, 3013, 3820
CciI TCATGA 2 cut(s) 1008, 2163
CfoI GCGC 5 cut(s) 1869, 2069, 2877, 3465, 3648
Cfr10I RCCGGY 2 cut(s) 154, 3306
Cfr13I GGNCC 8 cut(s) 152, 212, 353, 1226, 1427, 1672, 3554, 3793
CseI GACGC 2 cut(s) 2149, 4198
CsiI ACCWGGT 1 cut(s) 2274
CspCI CAANNNNNGTGG 2 cut(s) 3654, 3689
DraI TTTAAA 2 cut(s) 45, 942
DriI GACNNNNNGTC 5 cut(s) 1333, 1425, 1512, 2021, 3258
EaeI YGGCCR 3 cut(s) 952, 3304, 4016
EagI CGGCCG 1 cut(s) 3304
Eam1104I CTCTTC 4 cut(s) 2316, 2839, 3333, 3626
Eam1105I GACNNNNNGTC 5 cut(s) 1333, 1425, 1512, 2021, 3258
EarI CTCTTC 4 cut(s) 2316, 2839, 3333, 3626
EciI GGCGGA 1 cut(s) 2883
Ecl136II GAGCTC 3 cut(s) 330, 3343, 3940
EclXI CGGCCG 1 cut(s) 3304
Eco130I CCWWGG 4 cut(s) 1912, 2026, 2864, 3851
Eco147I AGGCCT 2 cut(s) 40, 2031
Eco24I GRGCYC 7 cut(s) 75, 254, 332, 2254, 2530, 3345, 3942
Eco31I GGTCTC 2 cut(s) 1531, 1933
Eco32I GATATC 1 cut(s) 2145
Eco47I GGWCC 2 cut(s) 1427, 1672
Eco52I CGGCCG 1 cut(s) 3304
Eco53kI GAGCTC 3 cut(s) 330, 3343, 3940
Eco57I CTGAAG 6 cut(s) 81, 885, 1535, 2253, 2520, 3435
Eco81I CCTNAGG 2 cut(s) 1254, 4197
EcoICRI GAGCTC 3 cut(s) 330, 3343, 3940
EcoNI CCTNNNNNAGG 1 cut(s) 3800
EcoO109I RGGNCCY 1 cut(s) 1672
EcoRI GAATTC 1 cut(s) 2083
EcoRII CCWGG 8 cut(s) 148, 1095, 1318, 1687, 2031, 2154, 2274, 3556
EcoRV GATATC 1 cut(s) 2145
EcoT14I CCWWGG 4 cut(s) 1912, 2026, 2864, 3851
EcoT22I ATGCAT 1 cut(s) 2647
EcoT38I GRGCYC 7 cut(s) 75, 254, 332, 2254, 2530, 3345, 3942
ErhI CCWWGG 4 cut(s) 1912, 2026, 2864, 3851
FalI AAGNNNNNCTT 4 cut(s) 1021, 1053, 2226, 2258
FaqI GGGAC 5 cut(s) 1115, 1432, 1602, 1658, 3980
FauI CCCGC 3 cut(s) 86, 160, 1645
FauNDI CATATG 4 cut(s) 916, 1389, 1503, 3184
FbaI TGATCA 4 cut(s) 10, 478, 541, 2422
FblI GTMKAC 1 cut(s) 2298
FriOI GRGCYC 7 cut(s) 75, 254, 332, 2254, 2530, 3345, 3942
FspBI CTAG 5 cut(s) 710, 788, 1092, 2403, 2507
GlaI GCGC 5 cut(s) 1868, 2068, 2876, 3464, 3647
GsaI CCCAGC 4 cut(s) 105, 354, 2183, 3444
GsuI CTGGAG 7 cut(s) 366, 843, 1957, 2205, 2268, 2544, 3969
HapII CCGG 5 cut(s) 155, 197, 1847, 3155, 3307
HgaI GACGC 2 cut(s) 2149, 4198
HhaI GCGC 5 cut(s) 1869, 2069, 2877, 3465, 3648
Hin1I GRCGYC 2 cut(s) 2160, 3246
Hin6I GCGC 5 cut(s) 1867, 2067, 2875, 3463, 3646
HinP1I GCGC 5 cut(s) 1867, 2067, 2875, 3463, 3646
HincII GTYRAC 2 cut(s) 1338, 2215
HindII GTYRAC 2 cut(s) 1338, 2215
HindIII AAGCTT 1 cut(s) 1356
HpaI GTTAAC 1 cut(s) 2215
HpaII CCGG 5 cut(s) 155, 197, 1847, 3155, 3307
HphI GGTGA 6 cut(s) 1364, 1400, 1671, 2379, 3797, 3817
Hpy166II GTNNAC 7 cut(s) 204, 697, 1338, 2215, 2299, 2673, 3715
Hpy8I GTNNAC 7 cut(s) 204, 697, 1338, 2215, 2299, 2673, 3715
Hpy99I CGWCG 2 cut(s) 773, 4192
HpyAV CCTTC 9 cut(s) 820, 1813, 1979, 2400, 2442, 3104, 3197, 3459, 3895
HpyCH4III ACNGT 8 cut(s) 232, 281, 1288, 2818, 3147, 3376, 3577, 3933
HpyCH4IV ACGT 3 cut(s) 5, 3246, 4126
HpyF10VI GCNNNNNNNGC 9 cut(s) 98, 220, 465, 1561, 2180, 3774, 4136, 4180, 4189
HpySE526I ACGT 3 cut(s) 5, 3246, 4126
Hsp92I GRCGYC 2 cut(s) 2160, 3246
HspAI GCGC 5 cut(s) 1867, 2067, 2875, 3463, 3646
KpnI GGTACC 1 cut(s) 1853
KroI GCCGGC 1 cut(s) 3306
KroNI GCCGGC 1 cut(s) 3308
Ksp22I TGATCA 4 cut(s) 10, 478, 541, 2422
KspAI GTTAAC 1 cut(s) 2215
LguI GCTCTTC 1 cut(s) 3333
LweI GCATC 9 cut(s) 210, 247, 772, 958, 1551, 1717, 2743, 3309, 4058
MabI ACCWGGT 1 cut(s) 2274
MaeI CTAG 5 cut(s) 710, 788, 1092, 2403, 2507
MaeII ACGT 3 cut(s) 5, 3246, 4126
MaeIII GTNAC 9 cut(s) 226, 275, 1282, 2385, 2454, 2767, 2780, 3577, 3731
MfeI CAATTG 2 cut(s) 2918, 2990
MflI RGATCY 7 cut(s) 775, 1168, 2038, 2860, 2947, 3031, 3757
MhlI GDGCHC 9 cut(s) 75, 254, 332, 2254, 2530, 3345, 3401, 3717, 3942
MlsI TGGCCA 1 cut(s) 954
MluNI TGGCCA 1 cut(s) 954
MlyI GAGTC 9 cut(s) 1522, 1570, 1891, 2031, 2294, 2825, 3185, 3643, 4040
MmeI TCCRAC 6 cut(s) 247, 637, 1776, 1812, 2519, 3216
Mox20I TGGCCA 1 cut(s) 954
Mph1103I ATGCAT 1 cut(s) 2647
MroNI GCCGGC 1 cut(s) 3306
MscI TGGCCA 1 cut(s) 954
MslI CAYNNNNRTG 7 cut(s) 243, 1211, 1593, 2061, 2584, 2754, 3519
Msp20I TGGCCA 1 cut(s) 954
MspA1I CMGCKG 3 cut(s) 101, 344, 1640
MspI CCGG 5 cut(s) 155, 197, 1847, 3155, 3307
MspR9I CCNGG 9 cut(s) 150, 1097, 1320, 1689, 1847, 2033, 2156, 2276, 3558
MunI CAATTG 2 cut(s) 2918, 2990
Mva1269I GAATGC 2 cut(s) 316, 2645
MvaI CCWGG 8 cut(s) 150, 1097, 1320, 1689, 2033, 2156, 2276, 3558
MvnI CGCG 2 cut(s) 1869, 2283
MwoI GCNNNNNNNGC 9 cut(s) 98, 220, 465, 1561, 2180, 3774, 4136, 4180, 4189
NaeI GCCGGC 1 cut(s) 3308
NciI CCSGG 1 cut(s) 1847
NcoI CCATGG 1 cut(s) 1912
NdeI CATATG 4 cut(s) 916, 1389, 1503, 3184
NgoMIV GCCGGC 1 cut(s) 3306
NmeAIII GCCGAG 1 cut(s) 240
NmuCI GTSAC 4 cut(s) 226, 2385, 3577, 3731
NruI TCGCGA 1 cut(s) 2283
NsiI ATGCAT 1 cut(s) 2647
NspI RCATGY 3 cut(s) 1733, 2381, 2788
NspV TTCGAA 1 cut(s) 371
OliI CACNNNNGTG 1 cut(s) 2584
PaeI GCATGC 1 cut(s) 2381
PagI TCATGA 2 cut(s) 1008, 2163
PasI CCCWGGG 2 cut(s) 149, 2155
PceI AGGCCT 2 cut(s) 40, 2031
PciSI GCTCTTC 1 cut(s) 3333
PctI GAATGC 2 cut(s) 316, 2645
PdiI GCCGGC 1 cut(s) 3308
PflMI CCANNNNNTGG 3 cut(s) 638, 2032, 2246
PleI GAGTC 9 cut(s) 1521, 1570, 1890, 2030, 2293, 2825, 3184, 3642, 4039
PpsI GAGTC 9 cut(s) 1521, 1570, 1890, 2030, 2293, 2825, 3184, 3642, 4039
Ppu21I YACGTR 1 cut(s) 4127
PpuMI RGGWCCY 1 cut(s) 1672
PshBI ATTAAT 1 cut(s) 1209
Psp124BI GAGCTC 3 cut(s) 332, 3345, 3942
Psp5II RGGWCCY 1 cut(s) 1672
Psp6I CCWGG 8 cut(s) 148, 1095, 1318, 1687, 2031, 2154, 2274, 3556
PspFI CCCAGC 4 cut(s) 101, 350, 2179, 3440
PspGI CCWGG 8 cut(s) 148, 1095, 1318, 1687, 2031, 2154, 2274, 3556
PspPI GGNCC 8 cut(s) 152, 212, 353, 1226, 1427, 1672, 3554, 3793
PspPPI RGGWCCY 1 cut(s) 1672
PsrI GAACNNNNNNTAC 2 cut(s) 3355, 3387
PstI CTGCAG 2 cut(s) 1183, 3315
PstNI CAGNNNCTG 3 cut(s) 1303, 3013, 3820
PsuI RGATCY 7 cut(s) 775, 1168, 2038, 2860, 2947, 3031, 3757
PvuII CAGCTG 2 cut(s) 101, 344
RruI TCGCGA 1 cut(s) 2283
RseI CAYNNNNRTG 7 cut(s) 243, 1211, 1593, 2061, 2584, 2754, 3519
SacI GAGCTC 3 cut(s) 332, 3345, 3942
SapI GCTCTTC 1 cut(s) 3333
Sau96I GGNCC 8 cut(s) 152, 212, 353, 1226, 1427, 1672, 3554, 3793
ScaI AGTACT 1 cut(s) 2505
SchI GAGTC 9 cut(s) 1522, 1570, 1891, 2031, 2294, 2825, 3185, 3643, 4040
ScrFI CCNGG 9 cut(s) 150, 1097, 1320, 1689, 1847, 2033, 2156, 2276, 3558
SduI GDGCHC 9 cut(s) 75, 254, 332, 2254, 2530, 3345, 3401, 3717, 3942
SexAI ACCWGGT 1 cut(s) 2274
SfaNI GCATC 9 cut(s) 210, 247, 772, 958, 1551, 1717, 2743, 3309, 4058
SfcI CTRYAG 4 cut(s) 339, 1179, 2460, 3311
SfuI TTCGAA 1 cut(s) 371
SinI GGWCC 2 cut(s) 1427, 1672
SmiI ATTTAAAT 1 cut(s) 942
SmiMI CAYNNNNRTG 7 cut(s) 243, 1211, 1593, 2061, 2584, 2754, 3519
SmlI CTYRAG 3 cut(s) 123, 1925, 2725
SmoI CTYRAG 3 cut(s) 123, 1925, 2725
SpeI ACTAGT 1 cut(s) 787
SphI GCATGC 1 cut(s) 2381
SseBI AGGCCT 2 cut(s) 40, 2031
SspI AATATT 1 cut(s) 472
SspMI CTAG 5 cut(s) 710, 788, 1092, 2403, 2507
SstI GAGCTC 3 cut(s) 332, 3345, 3942
StuI AGGCCT 2 cut(s) 40, 2031
StyD4I CCNGG 9 cut(s) 148, 1095, 1318, 1687, 1845, 2031, 2154, 2274, 3556
StyI CCWWGG 4 cut(s) 1912, 2026, 2864, 3851
SwaI ATTTAAAT 1 cut(s) 942
TaaI ACNGT 8 cut(s) 232, 281, 1288, 2818, 3147, 3376, 3577, 3933
TaiI ACGT 3 cut(s) 8, 3249, 4129
TaqI TCGA 6 cut(s) 371, 497, 1579, 2014, 3754, 4187
TatI WGTACW 6 cut(s) 1546, 1855, 2503, 2936, 3544, 4156
TauI GCSGC 1 cut(s) 4018
TscAI CASTG 9 cut(s) 556, 1150, 1188, 3018, 3150, 3406, 3825, 3952, 4111
TseFI GTSAC 4 cut(s) 226, 2385, 3577, 3731
Tsp45I GTSAC 4 cut(s) 226, 2385, 3577, 3731
TspDTI ATGAA 7 cut(s) 789, 839, 1617, 2111, 2217, 2580, 3216
TspGWI ACGGA 2 cut(s) 1075, 1442
TspRI CASTG 9 cut(s) 556, 1150, 1188, 3018, 3150, 3406, 3825, 3952, 4111
Van91I CCANNNNNTGG 3 cut(s) 638, 2032, 2246
VneI GTGCAC 1 cut(s) 3713
VpaK11BI GGWCC 2 cut(s) 1427, 1672
VspI ATTAAT 1 cut(s) 1209
XagI CCTNNNNNAGG 1 cut(s) 3800
XapI RAATTY 6 cut(s) 16, 1048, 2083, 2309, 2869, 3274
XceI RCATGY 3 cut(s) 1733, 2381, 2788
XmiI GTMKAC 1 cut(s) 2298
XspI CTAG 5 cut(s) 710, 788, 1092, 2403, 2507
ZraI GACGTC 1 cut(s) 3247
ZrmI AGTACT 1 cut(s) 2505
Zsp2I ATGCAT 1 cut(s) 2647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.