MD02G1013000.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004627
Physical Location & Seq
Reverse (-)
826828 .. 833253
6426 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1013000.v1.1.491

Sequence Viewer

Length: 4161 bp
ATGGCGTTTGATCAAAATTCGTTTCCCAAAGAACTGAGACAGTTAAATGTAGCTCGAACTGTGGCCGAAGAACCCCGCATTGCGCAGGCTACTACAGCTGCTCGAAACCCCGATGGATTTTTCCCCAGCCACCCAGTTCGTGAAGTTAATAGCCCTCATTCAATACCTGTTTTTTACCAAGCACCGGTTGCCTCGGAGGCTGGGCATGTTGGCCTAGGGTATGGAAATGCCGTGTCGGGGGTGCCCCCGTGGTGCCCTGGCCCCCGCATTGCTGTGCCGGTGGGACATCCAGGTGTGAACCCTGGCGTTGGTGTTGGAATTGGTTACAGTCCTAATTTGGGCAGTCGGGTCGGCGGAAATGATGTTGATCTTGTAAGCTCTGATACTACAACAACTGCTTCTAGGCCTTCTGACTGTAGTTCGAATATGTTTAATAGGAGTGCTGGCAGTGGTCTAGATCATGGAGTTAATGATATGGCAGCTGGGTTCGGTTATAATCCCAATTTAGGCAATAAGGTCAGTGGCAATGTTGCTGATCAGACTGGGAATGATTTGACATCGGGATATGGTTACAATGCCAATTTGGGCAGTCGTGGGAGTGGGACTGAACAAGCAAGTGATGACGGTGGGGAAGATTCTGTGTATGGGAAGAAAATAAAGCTTCTGTGCAGCTTTGGCGGAAAGATTTTGCCCAGACCAAGTGATGGAATGTTGAGGTATGTTGGAGGGCAGACAAGGATTATTAGTGTTCGGAGAGATGTGAGCTTCAATGAGCTAGTGCAGAAGATGGTGGATACATATGGGCAACCTGTGGTCATTAAATACCAGCTCCCCGATGAGGATCTCGATGCACTGGTGTCGGTTTCATGTGCTGATGATCTTGACAATATGAAGGATGAATATGGGAAGTTGGTCGAGAGATCTCCAGATGGTTCAGCAAAGTTGAGGGTATTTTTGTTTTCTGCTTCTGAAGTTGATCCATCTGGTGTCGTACAAATTGGGGATTTACATAATAGTGAGCAGAGATATGTTGATGCTGTCAATGGGATTATGGATGGGGTGAGTGTTGGTATCGTGAGGAAGGAGAGCAATGCAAGTGCAACTTCAACTCAAAATTCTGATTTTAGTGGAACTGATGTTGTTGATAACTCAGTTCCTGGTCAGGGGGACAATACTGGACTTCCATCACCGGGCAAGTTATCACCGAAAAGGGATTCATCTACTTCTCATGATGCTAGTACAAGGCTGGTATTTGTGGATCCTAAGCCTACAATTTATTCTGAAGTTTCTACAATTCCATTGGTCATTCCTGTGGTTAAGTCTGGGCCTCCCCAAACACCATCTTCTCATCCGGAAGGTGAATTAGAAAGATCTGTGCCTGTTACAGTATCACAGCAGCAACCTGGAATTGGCATTTCATCTACTGCTCCTTATCTGCAAACTTATGTTGGTCCTCGCCAAGATTTTGTTACACGGGCAGACCATCTCCAGCTTCCTCCTCAGATGGGTTTCCCAAGTCCTCACCTATTGGGGACTGCTGGTCCTGTATTCACCAAACAGCAATACGGTGATAGTATTGCTGGCATCACCCCGCATCACTTCATTCCTGCAGTGCACATGACAATGACTCCCTCGACTTCTCATGTTAATATAAGACCAAGTGTGCTTCAGCCATTGATCCATCCACAACAGACCCGGTTGGATAAGTATGTAGGCGAGAGTACCTTTGTTCCCAAGGTCGTCCAGCTTCCTGCTGAACAAAGCTATAATTCCTATCAGGTTCAAGTACCCTCAACAACAGCAGGAGGTGCTTATGGATGGCATCAAGTTCAATCACCAGAGCATGTGATTTACCATGATGCATTGGTATCTCACCAACAGGAAGTTCAGAGAGACATTGGCGGAAGCCCGGTATCTGATTCAAGTCCAACTTATTATAGTCCTCGGCTGGAGGAGAATTTGCGAGCTCAGCCGATGAACAAGGGAGAAGGTAATTTTGGCCAAGGAGTTGAGGCTCGCCCTAGGGTTCAAATTCCAGTAGATCCTCTTCTCGTAACATCTCATTCAGATGTGACTTGCCTCTCTCAGATTTCATCTCCACAGGGCGTTATCCAGAGAGGAGGTGATGTGCAATCACCAGATGGTGCATTCATGGTCAGTATTCCTAAGCATTGTCAAGACGATGTTGTTCAACTACATCCAACACCATTTCATTACCAGGTTAAACAGGAAAATTTAGGTAACGATCCTTTTAATCAAGATGTACCTCTTGTTGGAGGTGCACCTGTTGAAACTTCAGAATGCCTGGTTCGTGAATCTCCAACTGCATATGATAATTTGAGACAAATCGACGGGAGGATGGAAACACTCAGGATAAATCCCACTGAGATTTATGTTAACAAGGAGCATGGTAAATCACCTTGGGATATACCTAGAATGGAAGACAACGTTGATCACAAAACAGCACAAGTTGGGGGTAGAGAGGTGGCTGTGGATAACCTCGTTGACAAATTAGGCAGGAATCACTTTAACCCGACCGAAGTACTAGCTTCTCCCTCTGCCGAAGTCTCACATGGGTATAGTTCACAGCCAGCAGAATTCTATGATTTAGGAAAACAATCTATATGGGGAAATCCTGAAGCTTATCTGCAATCAAGAGTTGGAATATATCCTTGTGATTCTTACGAATCTAATTATGGAACCCCTGTTGTTTTGACCCATCTCACTGATGGAATTCAACCTCCTCCTGAATGGAAGGATGAGAATATGAGATTGCAGCCCAAGATGGTATCTAACGAGAGGGAAGGTATTACCTCGAATGGTTTTGGTGCCCAGGACTCCTCAAACTCACTGTTCAGCAATCAAGATCCTTGGAGTTTGTGCCAGGATACTCAATTGCCTCCTAAGCCAACCAAAATACAATTGAGAAAAGAACCATATACAGAAATGCGAATGGACGATGGAGGCCAACAGCCACCAGGCAATTTGAACAGGGATCTTAGTTCAGAGCATGCCCCGTCGTCCAAAGGATCAGCAGAAGAACAAATAAAGCAAGAACTTCAAGCTGTTGCTGAGGGAGTAGCTGCTTGTGTTTTTCAGTCATCTACGCCATCAGATTCTTACTTGCATGATAAAAATGAGTCTGCTTATGCAACCAATCGAAATGAAGAGGTTCAAAACAATGCTGCAGGGATGCAAAACAGAACAGAAAGTGAGGATGTCAAATCCAACTTTGCAGATAAGCCAAATCTTGGTTTTCCAGTAGCAGATGGCCTAGGCCGGTTGCAGATTATAAAGAACAGTGACCTTGAAGAGATGAGAGAATTAGGTTCTGGAACTTTTGGTACCGTTTATCATGGAAAATGGAGGGGCACTGATGTTGCAATCAAAAGAATCAATGATAGGTGTTTTTCCGGGAAGCCTTCAGAACAAGAGCGCATGAGAGATGATTTCTGGAATGAGGCTATCAAGCTTGCTGACTTGCACCACCCCAATGTGGTAGCTTTCTATGGGATTGTGCTTGACGGACCCGGAGGTTCTGTGGCAACAGTGACAGAATATATGGTTAACGGCTCTCTGAGAAATGCCTTGCAGAAAAATGAGAATTTGGACAGGCGCAAGCGCCTCATGATTGCAATGGATGTGGCATTCGGAATGGAGTACCTGCATGAAAAGAAGATAGTACACTTTGATTTGAAAAGTGACAACTTACTTGTCAATCTTCGAGATCCACACCGCCCAATATGCAAGGTCGGTGATTTGGGCCTATCTAAGGTGAAATGTCAAACGCTGATCTCCGGTGGTGTGCGGGGAACACTTCCATGGATGGCACCGGAGCTGTTGAATGGTAGCAGTAGCCTTGTTTCTGAGAAGGTCGATGTGTTTTCTTTCGGTATCATGTTGTGGGAGCTGCTCACTGGAGACGAACCATATGCGGATTTGCACTATGGTGCTATCATAGGTGGTATTGTGAGCAACACATTGCGGCCACCAATACCGGACTCTTGCGATCCTGAATGGAAGTCACTGATGGAGAGATGCTGGTCATCAGAGCCGTCCGAGAGGCTGAGTTTCACTGAAATTGCGAACGGATTACGCTCCATGGCAGCAAAGGTTCCTCCTAAAGGACAGACCCAACCGCAGCAACCCTCGGCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1387

Amino Acids

150.58

Weight (kDa)

5.27

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 238 - 321 2e-15 PB1 domain
PK_Tyr_Ser-Thr PF07714 1104 - 1363 5.1e-59 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 1104 - 1362 2.6e-47 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 495, 3293
Acc16I TGCGCA 1 cut(s) 84
Acc36I ACCTGC 1 cut(s) 3672
Acc65I GGTACC 1 cut(s) 3344
AccB1I GGYRCC 5 cut(s) 241, 252, 2828, 3344, 3829
AccB7I CCANNNNNTGG 2 cut(s) 704, 3251
AccIII TCCGGA 1 cut(s) 1351
AclI AACGTT 1 cut(s) 2448
AcoI YGGCCR 3 cut(s) 63, 1999, 3986
AcsI RAATTY 8 cut(s) 16, 1114, 1957, 2031, 2233, 2597, 2733, 3604
AcuI CTGAAG 6 cut(s) 990, 1302, 1652, 2280, 2658, 3408
AfaI GTAC 9 cut(s) 993, 1240, 1723, 1788, 2265, 2544, 3346, 3662, 3684
AgeI ACCGGT 1 cut(s) 184
AhdI GACNNNNNGTC 1 cut(s) 1539
AjuI GAANNNNNNNTTGG 4 cut(s) 1869, 1901, 1980, 2012
AleI CACNNNNGTG 1 cut(s) 3948
Alw21I GWGCWC 3 cut(s) 1617, 1969, 2284
Alw26I GTCTC 5 cut(s) 31, 1887, 2335, 2572, 3915
Alw44I GTGCAC 2 cut(s) 1613, 2280
AlwNI CAGNNNCTG 1 cut(s) 1157
Aor13HI TCCGGA 1 cut(s) 1351
ApaLI GTGCAC 2 cut(s) 1613, 2280
ApoI RAATTY 8 cut(s) 16, 1114, 1957, 2031, 2233, 2597, 2733, 3604
AsiGI ACCGGT 1 cut(s) 184
Asp700I GAANNNNTTC 1 cut(s) 3171
Asp718I GGTACC 1 cut(s) 3344
AspA2I CCTAGG 3 cut(s) 214, 2021, 3274
AspLEI GCGC 4 cut(s) 85, 3438, 3618, 3624
AspS9I GGNCC 6 cut(s) 260, 1325, 1451, 1541, 3527, 3763
AsuC2I CCSGG 5 cut(s) 1191, 1696, 1910, 3415, 3531
AsuII TTCGAA 1 cut(s) 422
AvaII GGWCC 3 cut(s) 1451, 1541, 3527
AvrII CCTAGG 3 cut(s) 214, 2021, 3274
BaeGI GKGCMC 6 cut(s) 246, 257, 1617, 2284, 2833, 3374
BalI TGGCCA 1 cut(s) 2001
BamHI GGATCC 1 cut(s) 1258
BanI GGYRCC 5 cut(s) 241, 252, 2828, 3344, 3829
BanII GRGCYC 1 cut(s) 1969
BarI GAAGNNNNNNTAC 2 cut(s) 2031, 2063
BbsI GAAGAC 1 cut(s) 2448
Bbv12I GWGCWC 3 cut(s) 1617, 1969, 2284
BbvCI CCTCAGC 1 cut(s) 3072
BceAI ACGGC 3 cut(s) 215, 3586, 4039
BcgI CGANNNNNNTGC 6 cut(s) 331, 365, 840, 874, 3914, 3948
BciVI GTATCC 2 cut(s) 787, 2881
BclI TGATCA 3 cut(s) 10, 535, 2452
BcnI CCSGG 5 cut(s) 1191, 1696, 1910, 3415, 3531
BcoDI GTCTC 5 cut(s) 31, 1887, 2335, 2572, 3915
BfaI CTAG 9 cut(s) 215, 402, 455, 776, 1236, 2022, 2433, 2546, 3275
BfmI CTRYAG 4 cut(s) 93, 415, 1608, 3186
BfoI RGCGCY 1 cut(s) 3625
BfuAI ACCTGC 1 cut(s) 3672
BfuI GTATCC 2 cut(s) 787, 2881
BglI GCCNNNNNGGC 1 cut(s) 197
BglII AGATCT 2 cut(s) 920, 1370
BlnI CCTAGG 3 cut(s) 214, 2021, 3274
BlpI GCTNAGC 1 cut(s) 1968
BmcAI AGTACT 1 cut(s) 2544
Bme18I GGWCC 3 cut(s) 1451, 1541, 3527
BmeRI GACNNNNNGTC 1 cut(s) 1539
BmgT120I GGNCC 6 cut(s) 260, 1325, 1451, 1541, 3527, 3763
BmrI ACTGGG 2 cut(s) 128, 552
BmsI GCATC 9 cut(s) 838, 1024, 1222, 1593, 1603, 1831, 1849, 3183, 4028
BmuI ACTGGG 2 cut(s) 128, 552
BpiI GAAGAC 1 cut(s) 2448
BpmI CTGGAG 4 cut(s) 909, 1472, 1970, 3939
Bpu10I CCTNAGC 4 cut(s) 1263, 2166, 2904, 3072
Bpu1102I GCTNAGC 1 cut(s) 1968
Bpu14I TTCGAA 1 cut(s) 422
BpuMI CCSGG 5 cut(s) 1191, 1696, 1910, 3415, 3531
BsaBI GATNNNNATC 2 cut(s) 366, 840
BsaWI WCCGGW 5 cut(s) 184, 1351, 3797, 3832, 3997
BsaXI ACNNNNNCTCC 6 cut(s) 1612, 1642, 2112, 2142, 3525, 3555
Bse118I RCCGGY 3 cut(s) 184, 277, 3279
Bse1I ACTGG 7 cut(s) 134, 547, 858, 1180, 2036, 3260, 3922
Bse3DI GCAATG 6 cut(s) 78, 267, 532, 1096, 3642, 3980
Bse8I GATNNNNATC 2 cut(s) 366, 840
BseAI TCCGGA 1 cut(s) 1351
BseJI GATNNNNATC 2 cut(s) 366, 840
BseMI GCAATG 6 cut(s) 78, 267, 532, 1096, 3642, 3980
BseNI ACTGG 7 cut(s) 134, 547, 858, 1180, 2036, 3260, 3922
BseRI GAGGAG 5 cut(s) 1488, 1967, 2133, 2733, 2830
BseSI GKGCMC 6 cut(s) 246, 257, 1617, 2284, 2833, 3374
BseYI CCCAGC 3 cut(s) 125, 200, 482
BsgI GTGCAG 2 cut(s) 688, 800
Bsh1285I CGRYCG 1 cut(s) 2538
BshNI GGYRCC 5 cut(s) 241, 252, 2828, 3344, 3829
BshTI ACCGGT 1 cut(s) 184
BsiEI CGRYCG 1 cut(s) 2538
BsiHKAI GWGCWC 3 cut(s) 1617, 1969, 2284
BslFI GGGAC 4 cut(s) 297, 616, 1181, 1546
BsmAI GTCTC 5 cut(s) 31, 1887, 2335, 2572, 3915
BsmBI CGTCTC 1 cut(s) 3915
BsmFI GGGAC 4 cut(s) 297, 616, 1181, 1546
BsmI GAATGC 3 cut(s) 2147, 2306, 3647
Bsp119I TTCGAA 1 cut(s) 422
Bsp1286I GDGCHC 7 cut(s) 246, 257, 1617, 1969, 2284, 2833, 3374
Bsp13I TCCGGA 1 cut(s) 1351
Bsp1720I GCTNAGC 1 cut(s) 1968
Bsp19I CCATGG 2 cut(s) 3821, 4101
BspEI TCCGGA 1 cut(s) 1351
BspHI TCATGA 2 cut(s) 1228, 3627
BspMAI CTGCAG 2 cut(s) 1612, 3190
BspMI ACCTGC 1 cut(s) 3672
BspT104I TTCGAA 1 cut(s) 422
BspT107I GGYRCC 5 cut(s) 241, 252, 2828, 3344, 3829
BsrDI GCAATG 6 cut(s) 78, 267, 532, 1096, 3642, 3980
BsrFI RCCGGY 3 cut(s) 184, 277, 3279
BsrI ACTGG 7 cut(s) 134, 547, 858, 1180, 2036, 3260, 3922
BssAI RCCGGY 3 cut(s) 184, 277, 3279
BssT1I CCWWGG 9 cut(s) 214, 1734, 2002, 2021, 2420, 2870, 3274, 3821, 4101
Bst6I CTCTTC 3 cut(s) 2052, 3162, 3306
BstAPI GCANNNNNTGC 2 cut(s) 188, 1808
BstBI TTCGAA 1 cut(s) 422
BstC8I GCNNGC 9 cut(s) 87, 445, 1582, 1965, 2017, 2592, 3012, 3474, 3620
BstDSI CCRYGG 3 cut(s) 248, 3821, 4101
BstENI CCTNNNNNAGG 2 cut(s) 3770, 4122
BstH2I RGCGCY 1 cut(s) 3625
BstHHI GCGC 4 cut(s) 85, 3438, 3618, 3624
BstMAI GTCTC 5 cut(s) 31, 1887, 2335, 2572, 3915
BstMCI CGRYCG 1 cut(s) 2538
BstMWI GCNNNNNNNGC 8 cut(s) 95, 188, 197, 675, 1808, 1969, 2905, 3744
BstNSI RCATGY 3 cut(s) 209, 1847, 3014
BstSFI CTRYAG 4 cut(s) 93, 415, 1608, 3186
BstSLI GKGCMC 6 cut(s) 246, 257, 1617, 2284, 2833, 3374
BstV2I GAAGAC 1 cut(s) 2448
BstX2I RGATCY 8 cut(s) 841, 920, 1258, 1370, 2041, 2866, 2995, 3727
BstYI RGATCY 8 cut(s) 841, 920, 1258, 1370, 2041, 2866, 2995, 3727
BsuI GTATCC 2 cut(s) 787, 2881
BtgI CCRYGG 3 cut(s) 248, 3821, 4101
BtsI GCAGTG 2 cut(s) 454, 1617
BveI ACCTGC 1 cut(s) 3672
Cac8I GCNNGC 9 cut(s) 87, 445, 1582, 1965, 2017, 2592, 3012, 3474, 3620
CaiI CAGNNNCTG 1 cut(s) 1157
CciI TCATGA 2 cut(s) 1228, 3627
CfoI GCGC 4 cut(s) 85, 3438, 3618, 3624
Cfr10I RCCGGY 3 cut(s) 184, 277, 3279
Cfr13I GGNCC 6 cut(s) 260, 1325, 1451, 1541, 3527, 3763
CsiI ACCWGGT 1 cut(s) 2217
Csp6I GTAC 9 cut(s) 992, 1239, 1722, 1787, 2264, 2543, 3345, 3661, 3683
CspAI ACCGGT 1 cut(s) 184
CspCI CAANNNNNGTGG 2 cut(s) 3624, 3659
CviQI GTAC 9 cut(s) 992, 1239, 1722, 1787, 2264, 2543, 3345, 3661, 3683
DriI GACNNNNNGTC 1 cut(s) 1539
EaeI YGGCCR 3 cut(s) 63, 1999, 3986
Eam1104I CTCTTC 3 cut(s) 2052, 3162, 3306
Eam1105I GACNNNNNGTC 1 cut(s) 1539
EarI CTCTTC 3 cut(s) 2052, 3162, 3306
EciI GGCGGA 3 cut(s) 369, 693, 1917
Ecl136II GAGCTC 1 cut(s) 1967
Eco130I CCWWGG 9 cut(s) 214, 1734, 2002, 2021, 2420, 2870, 3274, 3821, 4101
Eco147I AGGCCT 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 1969
Eco47I GGWCC 3 cut(s) 1451, 1541, 3527
Eco53kI GAGCTC 1 cut(s) 1967
Eco57I CTGAAG 6 cut(s) 990, 1302, 1652, 2280, 2658, 3408
EcoICRI GAGCTC 1 cut(s) 1967
EcoNI CCTNNNNNAGG 2 cut(s) 3770, 4122
EcoRI GAATTC 2 cut(s) 2597, 2733
EcoT14I CCWWGG 9 cut(s) 214, 1734, 2002, 2021, 2420, 2870, 3274, 3821, 4101
EcoT22I ATGCAT 1 cut(s) 1864
EcoT38I GRGCYC 1 cut(s) 1969
ErhI CCWWGG 9 cut(s) 214, 1734, 2002, 2021, 2420, 2870, 3274, 3821, 4101
Esp3I CGTCTC 1 cut(s) 3915
FalI AAGNNNNNCTT 4 cut(s) 1087, 1119, 1915, 1947
FaqI GGGAC 4 cut(s) 297, 616, 1181, 1546
FauI CCCGC 4 cut(s) 83, 272, 1599, 3801
FauNDI CATATG 3 cut(s) 799, 2329, 3931
FbaI TGATCA 3 cut(s) 10, 535, 2452
FriOI GRGCYC 1 cut(s) 1969
FspBI CTAG 9 cut(s) 215, 402, 455, 776, 1236, 2022, 2433, 2546, 3275
FspI TGCGCA 1 cut(s) 84
GlaI GCGC 4 cut(s) 84, 3437, 3617, 3623
GsaI CCCAGC 3 cut(s) 129, 204, 486
GsuI CTGGAG 4 cut(s) 909, 1472, 1970, 3939
HaeII RGCGCY 1 cut(s) 3625
HhaI GCGC 4 cut(s) 85, 3438, 3618, 3624
Hin6I GCGC 4 cut(s) 83, 3436, 3616, 3622
HinP1I GCGC 4 cut(s) 83, 3436, 3616, 3622
HincII GTYRAC 3 cut(s) 2398, 2506, 3568
HindII GTYRAC 3 cut(s) 2398, 2506, 3568
HindIII AAGCTT 3 cut(s) 659, 2640, 3470
HpaI GTTAAC 2 cut(s) 2398, 3568
Hpy166II GTNNAC 8 cut(s) 298, 1615, 2282, 2398, 2506, 2585, 3568, 3685
Hpy8I GTNNAC 8 cut(s) 298, 1615, 2282, 2398, 2506, 2585, 3568, 3685
Hpy99I CGWCG 2 cut(s) 2354, 3022
HpyAV CCTTC 9 cut(s) 417, 886, 1075, 1349, 1982, 2749, 2798, 3432, 3865
HpyCH4IV ACGT 1 cut(s) 2448
HpyF10VI GCNNNNNNNGC 8 cut(s) 95, 188, 197, 675, 1808, 1969, 2905, 3744
HpySE526I ACGT 1 cut(s) 2448
HspAI GCGC 4 cut(s) 83, 3436, 3616, 3622
Kpn2I TCCGGA 1 cut(s) 1351
KpnI GGTACC 1 cut(s) 3348
Ksp22I TGATCA 3 cut(s) 10, 535, 2452
KspAI GTTAAC 2 cut(s) 2398, 3568
LmnI GCTCC 6 cut(s) 834, 1432, 2404, 3835, 3907, 4103
LweI GCATC 9 cut(s) 838, 1024, 1222, 1593, 1603, 1831, 1849, 3183, 4028
MabI ACCWGGT 1 cut(s) 2217
MaeI CTAG 9 cut(s) 215, 402, 455, 776, 1236, 2022, 2433, 2546, 3275
MaeII ACGT 1 cut(s) 2448
MfeI CAATTG 2 cut(s) 2894, 2921
MflI RGATCY 8 cut(s) 841, 920, 1258, 1370, 2041, 2866, 2995, 3727
MhlI GDGCHC 7 cut(s) 246, 257, 1617, 1969, 2284, 2833, 3374
MlsI TGGCCA 1 cut(s) 2001
MluNI TGGCCA 1 cut(s) 2001
MlyI GAGTC 4 cut(s) 1621, 2831, 3149, 3995
MmeI TCCRAC 9 cut(s) 295, 703, 1680, 1952, 2225, 2254, 2345, 2641, 3252
Mox20I TGGCCA 1 cut(s) 2001
Mph1103I ATGCAT 1 cut(s) 1864
MroI TCCGGA 1 cut(s) 1351
MroXI GAANNNNTTC 1 cut(s) 3171
MscI TGGCCA 1 cut(s) 2001
MslI CAYNNNNRTG 9 cut(s) 272, 291, 1014, 1310, 1622, 2067, 3492, 3820, 3948
Msp20I TGGCCA 1 cut(s) 2001
MspA1I CMGCKG 2 cut(s) 98, 482
MunI CAATTG 2 cut(s) 2894, 2921
Mva1269I GAATGC 3 cut(s) 2147, 2306, 3647
MwoI GCNNNNNNNGC 8 cut(s) 95, 188, 197, 675, 1808, 1969, 2905, 3744
NciI CCSGG 5 cut(s) 1191, 1696, 1910, 3415, 3531
NcoI CCATGG 2 cut(s) 3821, 4101
NdeI CATATG 3 cut(s) 799, 2329, 3931
NmeAIII GCCGAG 2 cut(s) 1924, 4130
NmuCI GTSAC 5 cut(s) 2071, 3302, 3550, 3701, 4023
NsbI TGCGCA 1 cut(s) 84
NsiI ATGCAT 1 cut(s) 1864
NspI RCATGY 3 cut(s) 209, 1847, 3014
NspV TTCGAA 1 cut(s) 422
OliI CACNNNNGTG 1 cut(s) 3948
PaeI GCATGC 1 cut(s) 3014
PagI TCATGA 2 cut(s) 1228, 3627
PceI AGGCCT 1 cut(s) 406
PctI GAATGC 3 cut(s) 2147, 2306, 3647
PdmI GAANNNNTTC 1 cut(s) 3171
PfeI GAWTC 9 cut(s) 635, 1214, 1919, 2315, 2521, 2678, 2687, 3116, 3393
PflMI CCANNNNNTGG 2 cut(s) 704, 3251
PfoI TCCNGGA 1 cut(s) 3413
PinAI ACCGGT 1 cut(s) 184
PleI GAGTC 4 cut(s) 1621, 2831, 3148, 3995
PpsI GAGTC 4 cut(s) 1621, 2831, 3148, 3995
PsiI TTATAA 2 cut(s) 495, 3293
Psp124BI GAGCTC 1 cut(s) 1969
Psp1406I AACGTT 1 cut(s) 2448
PspFI CCCAGC 3 cut(s) 125, 200, 482
PspPI GGNCC 6 cut(s) 260, 1325, 1451, 1541, 3527, 3763
PsrI GAACNNNNNNTAC 2 cut(s) 3328, 3360
PstI CTGCAG 2 cut(s) 1612, 3190
PstNI CAGNNNCTG 1 cut(s) 1157
PsuI RGATCY 8 cut(s) 841, 920, 1258, 1370, 2041, 2866, 2995, 3727
PvuII CAGCTG 2 cut(s) 98, 482
RsaI GTAC 9 cut(s) 993, 1240, 1723, 1788, 2265, 2544, 3346, 3662, 3684
RsaNI GTAC 9 cut(s) 992, 1239, 1722, 1787, 2264, 2543, 3345, 3661, 3683
RseI CAYNNNNRTG 9 cut(s) 272, 291, 1014, 1310, 1622, 2067, 3492, 3820, 3948
SacI GAGCTC 1 cut(s) 1969
Sau96I GGNCC 6 cut(s) 260, 1325, 1451, 1541, 3527, 3763
ScaI AGTACT 1 cut(s) 2544
SchI GAGTC 4 cut(s) 1621, 2831, 3149, 3995
SduI GDGCHC 7 cut(s) 246, 257, 1617, 1969, 2284, 2833, 3374
SexAI ACCWGGT 1 cut(s) 2217
SfaNI GCATC 9 cut(s) 838, 1024, 1222, 1593, 1603, 1831, 1849, 3183, 4028
SfcI CTRYAG 4 cut(s) 93, 415, 1608, 3186
SfuI TTCGAA 1 cut(s) 422
SinI GGWCC 3 cut(s) 1451, 1541, 3527
SmiMI CAYNNNNRTG 9 cut(s) 272, 291, 1014, 1310, 1622, 2067, 3492, 3820, 3948
SphI GCATGC 1 cut(s) 3014
SseBI AGGCCT 1 cut(s) 406
SspMI CTAG 9 cut(s) 215, 402, 455, 776, 1236, 2022, 2433, 2546, 3275
SstI GAGCTC 1 cut(s) 1969
StuI AGGCCT 1 cut(s) 406
StyI CCWWGG 9 cut(s) 214, 1734, 2002, 2021, 2420, 2870, 3274, 3821, 4101
TaiI ACGT 1 cut(s) 2451
TaqII GACCGA 1 cut(s) 2552
TatI WGTACW 3 cut(s) 1238, 2542, 3682
TauI GCSGC 1 cut(s) 3988
TfiI GAWTC 9 cut(s) 635, 1214, 1919, 2315, 2521, 2678, 2687, 3116, 3393
TseFI GTSAC 5 cut(s) 2071, 3302, 3550, 3701, 4023
Tsp45I GTSAC 5 cut(s) 2071, 3302, 3550, 3701, 4023
TspGWI ACGGA 2 cut(s) 3540, 4104
Van91I CCANNNNNTGG 2 cut(s) 704, 3251
VneI GTGCAC 2 cut(s) 1613, 2280
VpaK11BI GGWCC 3 cut(s) 1451, 1541, 3527
XagI CCTNNNNNAGG 2 cut(s) 3770, 4122
XapI RAATTY 8 cut(s) 16, 1114, 1957, 2031, 2233, 2597, 2733, 3604
XbaI TCTAGA 1 cut(s) 454
XceI RCATGY 3 cut(s) 209, 1847, 3014
XcmI CCANNNNNNNNNTGG 1 cut(s) 2726
XmaJI CCTAGG 3 cut(s) 214, 2021, 3274
XmnI GAANNNNTTC 1 cut(s) 3171
XspI CTAG 9 cut(s) 215, 402, 455, 776, 1236, 2022, 2433, 2546, 3275
ZrmI AGTACT 1 cut(s) 2544
Zsp2I ATGCAT 1 cut(s) 1864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.