MD15G1159900.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004627
Physical Location & Seq
Reverse (-)
11956978 .. 11963068
6091 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1159900.v1.1.491

Sequence Viewer

Length: 4191 bp
ATGGCGTTTGATCAAAATTCGTTCCCCAAAGAATTGAGACCGTTGAATGTAGCTCGAACTGTGGCCGAAGAGCCCCGCATTGCTCAGGCTACTACAGCTGGTCGAAACCCTGATAGATTTTTCCCTAGCCACTTGGTTCGTGAAGTTAACAGCCCTAATTCACTACCTGTTTTTTACCAAGCACCGGTGGCCTCGGAGGCTGGGCATGTTGGCCTAGGCTATGTAAATGCCGTGTCGGGGGTGCCCACCTGGTGCCCTGGCCCCCGCATTGCTGTGCCGGTGGGGCATCCAGATGTGAACCCTTCTGTTGGTGTTGGAATTGGTTACAGTCCTAATTTGGGCAGTCGGGTTGGTGGCAGTGCTGTTGATCTTGTATGCTCTGCTACTACAACAACTGCTTCTGGGCCGTCTGGCTGTAGTTCGAACATGTGTAATAGGAGTGCTGGCAGTGGTTTAGATCATGGAGTTAATGATATGACAACTAGGTTCGGCTATAATCCCAATTTAGGCAATAAGGTCAGTGGCAATGTTGCTGATCAGACTGGAAATGATTTGACATCGGGGTATGGTTATAATGCCAATTTGGGCAGTCGTGGCAGTGGGACTGATCAAGCAAGTGATGACGGTGGAGATGATTCTGTGTCTGGGAAGAAAATAAAGCTTCTGTGCAGCTTTGGCGGAAAGATTTTGCCCAGACCAAGTGATGGAATGTTGAGGTATGTTGGAGGGCATACAAGGATTATTAGTGTTCGGAGAGATGTGAGCTTCAATGATCTAGTGCAGAAGATGGTGGATACATATGGGCAGCCTGTGGTTATTAAATACCAGCTCCCCGATGAGGATCTGGATGCACTGGTGTCAGTTTCATGTGCTGATGATCTTGACAATATGAAGGATGAGTATGGGAAGTTGGTCGAGAGATCTCCAGACGGGTCAGCAAAGTTGAGGGTATTTTTGTTTTCTGCTTCGGAAGTTGATCCATCTGGTGTTGTACAGTTGGGGGATTTACATAATAGTGAGCAGAGATATGTTGATGCTGTCAATGGGATTACGGATGGGGTGAGTGTTGGTATTGCTAGGAAGGAGAGCAATGCAAGTGCAGCTTCAACACAAAATTCTGATTTTAGTGGAACTGATGTTGTTGATAACTCAGTTCCTGGTCAAGGGGACAATACTGGACTTCCATCAGCGGGCAAGTTATCACCAAAAGGGGATTCAGCTACTTCTCATGACACTAGTACAAGGCTGGTATTTGTGGATCCTAAGCCTGCAATTTATTCTGAAGTTTCTACAATTCCATTGGTCATTCCTGTAGTTAAGTCTGGGCCTCCCCAAACACCATCTTCTCAGCCAGAGGGTGAGTTAGAAAGATCTGTGCCCGTTACAGTATCACAGCAGCAACTGGGATTGCAGCAACCGGGATTGCAGCAACCTGGAATTGGCATTTCATCCGCTGCTCCTTATCTGCAAACTTATGTTGGTCCTCGCCAAGAAGTTATGACACAGGCAGATCATATCCAGCTTCCTCCTCAGATGGGTTTCCCAAGTCCTCATCCAATGGGGACTGCTCGTCCTGTATTCACCCAACAGCAATATGGTGATAGTGTTGCTGGCATCACGCCGCATCACTTCATTCCTGCAGTGCACATGACAATGACTCCCTCGTATTCTCATGTTAATATAAGACCAAGTGTGCTTCAGCCATTGATCCATCCTCAACAGGCTTGGTTGGATAATTATGTAGACAAGAATACCTTTGTTCCCAGGGTCGTCCAGCTTCCGGCTGAACAAAGCTATAATTCCTATCAGGTTCAAGTACCGTCAACAACAGCAGGAGGTGCTTACGGATGGCATCAGGTTCAACCACCAGAGCATGTGATTTACCGTGATGGATTGGTATCTCACCAACAGGTTCAGAGAGACATTGGCGGAAGCCCAGTATCTGATTCAAGTCCAACTTATTATAGTCCTCGGCTAGAGGAGAATTTGCTAGCTCAGCCGATGAACAAGGTTATTGTAAGTGGACCACTAGGAGAAGGTAATTTTGGCCAAGGCGTTGAGGCTCAACCCAGGGTTCAAGTTCCAGTAGATCCTCTTCTCATAACATCTTATTCAAATGAGACTGGCCTCTCCCAGATTTCAACTCCCGAGGGCGTTAGCCAGAGAGGAGGTGACGTGCAATCACCGAATAGTGCATTCATGGTCAATACTCCTCAGCGTCGTCAAGACAATGTTGTTCAACAACATCCAACGCCATTTCAGTACCAGGTTAAACAGGAAAATTTAGTTAATGATCCTTTTAATCAAGATGTACCTCTTGATGGAGGCGCACCTGTGGTTTGTGAATCTCCAACTGCATATGATCATTTGAGACAAATCAACGGGAGGATGGAGGCCCTCAGGATGAATCCCACTGAGATTTATGTTAATAAGGAGCATGGTAAATCTCCTGGGGATACACCTAGAAGGGAAGACAGCTTTGATCATAAAACACCACAAGTTGGGGGTAGAGATGTGGCTCTAGATAACATCGTTGACAAATTTGGCAGGAATCATTTTAAGCCAACCAAAGTATCAGCTTCTTCCCCTGCCGAAGTCTCACATGGGTATAGTTCACAGCCAGTAGAATTCTGTGATTTAGGACAGCAATCTATGTGGGGCAATCCTGAATCTTATCTGCAATCAAGAGTTGGAATGCATCCTCGTGATTCGTACGAATCTAACTATGGAAACCCTGTTGTTTCTACCCATCTCACTAATGGAATTCAACCTCCTGAATGGGAGGATGAGAACATGAGATTGCAGCCCAAGATGGTACCTAACAAGAGGGAAGTTATTACCTCAAATGGTGTCGGTGCCCAGGACTCCTCAAACTCATTGTTCAGCAATCAGGATCCTTGGAGTTTGCGACACGATACTCAATTGCCTCCTAGGCCGACCAAAATACAATTGAGAAAAGAACCATTTACAGAAATGCAAATGGATGGTGGAGGACAACAGCCAATGGGTAATTTGAACAGGGATCTTAGTTCAGAGGATGCCCCGTCTTCCAAAGGATCAGCAGAAGAACAAATAAAGCAAGAACTTCAAGCTGTTGCTGAGGGAGTAGCTGCCTGTGTTTTTCAGTCATTTACGCCATCAGATTCTTACTTGCTTGATAAAAATGAGTCTGCTTATGAATCCAATCGAGATGAAGAGGTTCAAAACAATGCTGCAGGGATGCAAAACAGATCGGAAAGTGAGGATGTCAAATTCAACTTTGCAGATAAGCCAAATCTTGGTTTTCCAGTAGCAGATGGCCTAGGCAGGTTGCAGATTATAAAGAACAGTGACCTTGAAGAGCTGAGAGAATTAGGTTCTGGAACTTTCGGTACTGTTTATCATGGAAAATGGAGGGGCACTGATGTTGCAATCAAAAGAATCAATGATAGGTGTTTTGCTGGAAAGCCTTCAGAACAGGAGCGCACGAGAGAGGATTTCTGGAATGAGGCCATCAAGCTTGCTGACTTGCACCACCCCAATGTGGTAGCTTTCTATGGCATTGTGCTTGACGGACCGGGAGGTTCTGTGGCAACAGTGACAGAATACATGGTTAACGGTTCTTTGAGAAATGCTTTGCAGAAAAATGAGAAGAGTCTGGACAAGCGCAAGCGCCTCATGATTGCCATGGATGTGGCATTCGGAATGGAGTACCTGCATGAAAAGAAGATAGTACACTTTGATTTGAAAAGTGACAACTTACTTGTCAATCTTCGAGATCCACACCGCCCAATATGCAAGGTCGGTGATTTGGGTCTATCTAAGGTGAAATGTCATACTCTGATCTCCGGTGGTGTGCGGGGAACACTTCCATGGATGGCACCAGAGCTGTTGAACGGTAGCAGTAGCCTTGTTTCTGAGAAGGTCGATGTATTTTCTTTCGGTATCGTGATGTGGGAGCTGCTGACTGGAGACGAACCATATGCGGATTTGCACTATGGTGCTATCATAGGTGGTATTGTGAGCAACACATTGCGGCCACCAGTACCGGACTTTTGCGATCCTGAATGGAAATCACTGATGGAGACATGCTGGTCAGCTGAGCCATCTGAGAGATTGAGTTTCACCGAAATTGCAAATGAGTTACGTGGTATGGCGGCAAAAATTCCTCCTAAAGGACAGACCCAACCGCAGCAACCCTCGCCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1397

Amino Acids

151.57

Weight (kDa)

5.32

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 238 - 321 1e-15 PB1 domain
Pkinase PF00069 1113 - 1373 7.9e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1114 - 1375 1.5e-60 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 573, 3320
AasI GACNNNNNNGTC 1 cut(s) 4072
Acc36I ACCTGC 2 cut(s) 3297, 3702
Acc65I GGTACC 1 cut(s) 2815
AccB1I GGYRCC 5 cut(s) 241, 252, 2815, 2855, 3859
AccB7I CCANNNNNTGG 3 cut(s) 704, 2501, 3278
AccI GTMKAC 1 cut(s) 1743
AcoI YGGCCR 3 cut(s) 63, 2047, 4016
AcsI RAATTY 9 cut(s) 16, 1114, 1984, 2281, 2540, 2627, 2763, 3251, 4143
AcuI CTGAAG 3 cut(s) 1302, 1682, 3435
AdeI CACNNNGTG 1 cut(s) 252
AflIII ACRYGT 1 cut(s) 426
AgeI ACCGGT 1 cut(s) 184
AhdI GACNNNNNGTC 1 cut(s) 1569
AhlI ACTAGT 1 cut(s) 1235
AjiI CACGTC 1 cut(s) 2176
AjnI CCWGG 9 cut(s) 248, 256, 1156, 1432, 1763, 2069, 2265, 2449, 2859
AjuI GAANNNNNNNTTGG 4 cut(s) 1198, 1230, 2028, 2060
AleI CACNNNNGTG 1 cut(s) 3978
Alw21I GWGCWC 1 cut(s) 1647
Alw26I GTCTC 7 cut(s) 31, 1914, 2114, 2365, 2602, 3945, 4058
Alw44I GTGCAC 1 cut(s) 1643
AlwNI CAGNNNCTG 3 cut(s) 1157, 1402, 1943
Ama87I CYCGRG 1 cut(s) 2147
ApaLI GTGCAC 1 cut(s) 1643
ApoI RAATTY 9 cut(s) 16, 1114, 1984, 2281, 2540, 2627, 2763, 3251, 4143
AsiGI ACCGGT 1 cut(s) 184
Asp700I GAANNNNTTC 2 cut(s) 3198, 3448
Asp718I GGTACC 1 cut(s) 2815
AspA2I CCTAGG 3 cut(s) 214, 2930, 3301
AspLEI GCGC 4 cut(s) 2330, 3465, 3648, 3654
AspS9I GGNCC 7 cut(s) 260, 404, 1325, 1481, 2024, 2395, 3554
AsuC2I CCSGG 2 cut(s) 1419, 3558
AsuII TTCGAA 1 cut(s) 422
AsuNHI GCTAGC 1 cut(s) 1990
AvaI CYCGRG 1 cut(s) 2147
AvaII GGWCC 3 cut(s) 1481, 2024, 3554
AvrII CCTAGG 3 cut(s) 214, 2930, 3301
AxyI CCTNAGG 1 cut(s) 2399
BaeGI GKGCMC 6 cut(s) 246, 257, 1380, 1647, 2860, 3401
BaeI ACNNNNGTAYC 2 cut(s) 2556, 2589
BalI TGGCCA 1 cut(s) 2049
BamHI GGATCC 2 cut(s) 1258, 2893
BanI GGYRCC 5 cut(s) 241, 252, 2815, 2855, 3859
BanII GRGCYC 1 cut(s) 75
BarI GAAGNNNNNNTAC 2 cut(s) 2079, 2111
BauI CACGAG 2 cut(s) 2703, 3466
BbsI GAAGAC 2 cut(s) 2478, 3039
Bbv12I GWGCWC 1 cut(s) 1647
BbvCI CCTCAGC 2 cut(s) 2214, 3099
BceAI ACGGC 2 cut(s) 215, 391
BcgI CGANNNNNNTGC 2 cut(s) 3944, 3978
BciT130I CCWGG 9 cut(s) 250, 258, 1158, 1434, 1765, 2071, 2267, 2451, 2861
BciVI GTATCC 2 cut(s) 787, 2449
BclI TGATCA 5 cut(s) 10, 535, 607, 2362, 2482
BcnI CCSGG 2 cut(s) 1419, 3558
BcoDI GTCTC 7 cut(s) 31, 1914, 2114, 2365, 2602, 3945, 4058
BcuI ACTAGT 1 cut(s) 1235
BfmI CTRYAG 5 cut(s) 93, 415, 1311, 1638, 3213
BfoI RGCGCY 1 cut(s) 3655
BfuAI ACCTGC 2 cut(s) 3297, 3702
BfuI GTATCC 2 cut(s) 787, 2449
BglI GCCNNNNNGGC 3 cut(s) 197, 283, 2932
BglII AGATCT 2 cut(s) 920, 1370
BlnI CCTAGG 3 cut(s) 214, 2930, 3301
BlpI GCTNAGC 2 cut(s) 1995, 4080
Bme18I GGWCC 3 cut(s) 1481, 2024, 3554
BmeRI GACNNNNNGTC 1 cut(s) 1569
BmeT110I CYCGRG 1 cut(s) 2147
BmgBI CACGTC 1 cut(s) 2176
BmgT120I GGNCC 7 cut(s) 260, 404, 1325, 1481, 2024, 2395, 3554
BmiI GGNNCC 8 cut(s) 243, 254, 262, 1260, 2817, 2857, 2895, 3861
BmrI ACTGGG 2 cut(s) 1412, 1931
BmsI GCATC 9 cut(s) 295, 838, 1024, 1623, 1633, 1861, 2707, 3028, 3210
BmtI GCTAGC 1 cut(s) 1994
BmuI ACTGGG 2 cut(s) 1412, 1931
BpiI GAAGAC 2 cut(s) 2478, 3039
BpmI CTGGAG 2 cut(s) 909, 3969
Bpu10I CCTNAGC 4 cut(s) 84, 1263, 2214, 3099
Bpu1102I GCTNAGC 2 cut(s) 1995, 4080
Bpu14I TTCGAA 1 cut(s) 422
BpuMI CCSGG 2 cut(s) 1419, 3558
BsaAI YACGTR 1 cut(s) 4127
BsaBI GATNNNNATC 2 cut(s) 840, 1897
BsaI GGTCTC 1 cut(s) 31
BsaWI WCCGGW 3 cut(s) 184, 3827, 4027
BsaXI ACNNNNNCTCC 6 cut(s) 1642, 1672, 2160, 2190, 3552, 3582
Bse118I RCCGGY 2 cut(s) 184, 277
Bse21I CCTNAGG 1 cut(s) 2399
Bse3DI GCAATG 5 cut(s) 78, 267, 532, 1096, 4010
Bse8I GATNNNNATC 2 cut(s) 840, 1897
BseBI CCWGG 9 cut(s) 250, 258, 1158, 1434, 1765, 2071, 2267, 2451, 2861
BseJI GATNNNNATC 2 cut(s) 840, 1897
BseMI GCAATG 5 cut(s) 78, 267, 532, 1096, 4010
BseRI GAGGAG 5 cut(s) 1518, 1994, 2181, 2202, 2857
BseSI GKGCMC 6 cut(s) 246, 257, 1380, 1647, 2860, 3401
BseYI CCCAGC 1 cut(s) 200
BsgI GTGCAG 3 cut(s) 688, 800, 1119
BshNI GGYRCC 5 cut(s) 241, 252, 2815, 2855, 3859
BshTI ACCGGT 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 1647
BsiHKCI CYCGRG 1 cut(s) 2147
BsiSI CCGG 7 cut(s) 185, 278, 1418, 1781, 3557, 3828, 4028
BsiWI CGTACG 1 cut(s) 2712
BslFI GGGAC 3 cut(s) 616, 1181, 1576
BsmAI GTCTC 7 cut(s) 31, 1914, 2114, 2365, 2602, 3945, 4058
BsmBI CGTCTC 1 cut(s) 3945
BsmFI GGGAC 3 cut(s) 616, 1181, 1576
BsmI GAATGC 3 cut(s) 2195, 2700, 3677
Bso31I GGTCTC 1 cut(s) 31
BsoBI CYCGRG 1 cut(s) 2147
Bsp119I TTCGAA 1 cut(s) 422
Bsp1286I GDGCHC 7 cut(s) 75, 246, 257, 1380, 1647, 2860, 3401
Bsp1407I TGTACA 1 cut(s) 991
Bsp1720I GCTNAGC 2 cut(s) 1995, 4080
Bsp19I CCATGG 2 cut(s) 3666, 3851
BspHI TCATGA 2 cut(s) 1228, 3657
BspLI GGNNCC 8 cut(s) 243, 254, 262, 1260, 2817, 2857, 2895, 3861
BspMAI CTGCAG 2 cut(s) 1642, 3217
BspMI ACCTGC 2 cut(s) 3297, 3702
BspOI GCTAGC 1 cut(s) 1994
BspQI GCTCTTC 2 cut(s) 63, 3333
BspT104I TTCGAA 1 cut(s) 422
BspT107I GGYRCC 5 cut(s) 241, 252, 2815, 2855, 3859
BspTNI GGTCTC 1 cut(s) 31
BsrDI GCAATG 5 cut(s) 78, 267, 532, 1096, 4010
BsrFI RCCGGY 2 cut(s) 184, 277
BsrGI TGTACA 1 cut(s) 991
BssAI RCCGGY 2 cut(s) 184, 277
BssSI CACGAG 2 cut(s) 2703, 3466
BssT1I CCWWGG 7 cut(s) 214, 2050, 2897, 2930, 3301, 3666, 3851
Bst2BI CACGAG 2 cut(s) 2703, 3466
Bst2UI CCWGG 9 cut(s) 250, 258, 1158, 1434, 1765, 2071, 2267, 2451, 2861
Bst6I CTCTTC 5 cut(s) 63, 2100, 3189, 3333, 3626
BstAPI GCANNNNNTGC 1 cut(s) 1838
BstAUI TGTACA 1 cut(s) 991
BstBAI YACGTR 1 cut(s) 4127
BstBI TTCGAA 1 cut(s) 422
BstC8I GCNNGC 7 cut(s) 445, 1192, 1269, 1612, 1992, 3501, 3650
BstDSI CCRYGG 2 cut(s) 3666, 3851
BstENI CCTNNNNNAGG 2 cut(s) 1161, 4152
BstH2I RGCGCY 1 cut(s) 3655
BstHHI GCGC 4 cut(s) 2330, 3465, 3648, 3654
BstMAI GTCTC 7 cut(s) 31, 1914, 2114, 2365, 2602, 3945, 4058
BstNI CCWGG 9 cut(s) 250, 258, 1158, 1434, 1765, 2071, 2267, 2451, 2861
BstNSI RCATGY 4 cut(s) 209, 430, 1877, 4071
BstSFI CTRYAG 5 cut(s) 93, 415, 1311, 1638, 3213
BstSLI GKGCMC 6 cut(s) 246, 257, 1380, 1647, 2860, 3401
BstV2I GAAGAC 2 cut(s) 2478, 3039
BstX2I RGATCY 8 cut(s) 841, 920, 1258, 1370, 2089, 2893, 3022, 3757
BstXI CCANNNNNNTGG 1 cut(s) 3673
BstYI RGATCY 8 cut(s) 841, 920, 1258, 1370, 2089, 2893, 3022, 3757
Bsu36I CCTNAGG 1 cut(s) 2399
BsuI GTATCC 2 cut(s) 787, 2449
BtgI CCRYGG 2 cut(s) 3666, 3851
BtrI CACGTC 1 cut(s) 2176
BtsI GCAGTG 4 cut(s) 364, 454, 604, 1647
BveI ACCTGC 2 cut(s) 3297, 3702
Cac8I GCNNGC 7 cut(s) 445, 1192, 1269, 1612, 1992, 3501, 3650
CaiI CAGNNNCTG 3 cut(s) 1157, 1402, 1943
CciI TCATGA 2 cut(s) 1228, 3657
CfoI GCGC 4 cut(s) 2330, 3465, 3648, 3654
Cfr10I RCCGGY 2 cut(s) 184, 277
Cfr13I GGNCC 7 cut(s) 260, 404, 1325, 1481, 2024, 2395, 3554
CpoI CGGWCCG 1 cut(s) 3554
CseI GACGC 1 cut(s) 2207
CsiI ACCWGGT 2 cut(s) 248, 2265
CspAI ACCGGT 1 cut(s) 184
CspCI CAANNNNNGTGG 2 cut(s) 2636, 2671
CspI CGGWCCG 1 cut(s) 3554
DraIII CACNNNGTG 1 cut(s) 252
DrdI GACNNNNNNGTC 1 cut(s) 4072
DriI GACNNNNNGTC 1 cut(s) 1569
DseDI GACNNNNNNGTC 1 cut(s) 4072
EaeI YGGCCR 3 cut(s) 63, 2047, 4016
Eam1104I CTCTTC 5 cut(s) 63, 2100, 3189, 3333, 3626
Eam1105I GACNNNNNGTC 1 cut(s) 1569
EarI CTCTTC 5 cut(s) 63, 2100, 3189, 3333, 3626
EciI GGCGGA 2 cut(s) 693, 1944
Eco130I CCWWGG 7 cut(s) 214, 2050, 2897, 2930, 3301, 3666, 3851
Eco24I GRGCYC 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 31
Eco47I GGWCC 3 cut(s) 1481, 2024, 3554
Eco57I CTGAAG 3 cut(s) 1302, 1682, 3435
Eco81I CCTNAGG 1 cut(s) 2399
Eco88I CYCGRG 1 cut(s) 2147
EcoNI CCTNNNNNAGG 2 cut(s) 1161, 4152
EcoO109I RGGNCCY 1 cut(s) 2395
EcoRI GAATTC 2 cut(s) 2627, 2763
EcoRII CCWGG 9 cut(s) 248, 256, 1156, 1432, 1763, 2069, 2265, 2449, 2859
EcoT14I CCWWGG 7 cut(s) 214, 2050, 2897, 2930, 3301, 3666, 3851
EcoT22I ATGCAT 1 cut(s) 2700
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 7 cut(s) 214, 2050, 2897, 2930, 3301, 3666, 3851
Esp3I CGTCTC 1 cut(s) 3945
FalI AAGNNNNNCTT 6 cut(s) 1087, 1119, 1739, 1771, 1942, 1974
FaqI GGGAC 3 cut(s) 616, 1181, 1576
FauI CCCGC 4 cut(s) 83, 272, 1183, 3831
FauNDI CATATG 3 cut(s) 799, 2359, 3961
FbaI TGATCA 5 cut(s) 10, 535, 607, 2362, 2482
FblI GTMKAC 1 cut(s) 1743
FriOI GRGCYC 1 cut(s) 75
GlaI GCGC 4 cut(s) 2329, 3464, 3647, 3653
GsaI CCCAGC 1 cut(s) 204
GsuI CTGGAG 2 cut(s) 909, 3969
HaeII RGCGCY 1 cut(s) 3655
HapII CCGG 7 cut(s) 185, 278, 1418, 1781, 3557, 3828, 4028
HgaI GACGC 1 cut(s) 2207
HhaI GCGC 4 cut(s) 2330, 3465, 3648, 3654
Hin6I GCGC 4 cut(s) 2328, 3463, 3646, 3652
HinP1I GCGC 4 cut(s) 2328, 3463, 3646, 3652
HincII GTYRAC 4 cut(s) 148, 1824, 2536, 3595
HindII GTYRAC 4 cut(s) 148, 1824, 2536, 3595
HindIII AAGCTT 2 cut(s) 659, 3497
HpaI GTTAAC 2 cut(s) 148, 3595
HpaII CCGG 7 cut(s) 185, 278, 1418, 1781, 3557, 3828, 4028
Hpy99I CGWCG 1 cut(s) 2223
HpyAV CCTTC 7 cut(s) 312, 886, 1075, 2030, 2460, 3459, 3895
HpyCH4IV ACGT 2 cut(s) 2175, 4126
HpySE526I ACGT 2 cut(s) 2175, 4126
HspAI GCGC 4 cut(s) 2328, 3463, 3646, 3652
KpnI GGTACC 1 cut(s) 2819
Ksp22I TGATCA 5 cut(s) 10, 535, 607, 2362, 2482
KspAI GTTAAC 2 cut(s) 148, 3595
LguI GCTCTTC 2 cut(s) 63, 3333
LmnI GCTCC 5 cut(s) 834, 1462, 2434, 3460, 3937
LweI GCATC 9 cut(s) 295, 838, 1024, 1623, 1633, 1861, 2707, 3028, 3210
MabI ACCWGGT 2 cut(s) 248, 2265
MaeII ACGT 2 cut(s) 2175, 4126
MaeIII GTNAC 7 cut(s) 323, 1381, 2171, 3329, 3577, 3731, 4122
MfeI CAATTG 2 cut(s) 2921, 2948
MflI RGATCY 8 cut(s) 841, 920, 1258, 1370, 2089, 2893, 3022, 3757
MhlI GDGCHC 7 cut(s) 75, 246, 257, 1380, 1647, 2860, 3401
MlsI TGGCCA 1 cut(s) 2049
MluNI TGGCCA 1 cut(s) 2049
MlyI GAGTC 4 cut(s) 1651, 2858, 3176, 3643
MmeI TCCRAC 7 cut(s) 295, 703, 1710, 1979, 2273, 2375, 2671
Mox20I TGGCCA 1 cut(s) 2049
Mph1103I ATGCAT 1 cut(s) 2700
MroXI GAANNNNTTC 2 cut(s) 3198, 3448
MscI TGGCCA 1 cut(s) 2049
MslI CAYNNNNRTG 9 cut(s) 272, 291, 1014, 1652, 2703, 3519, 3671, 3850, 3978
Msp20I TGGCCA 1 cut(s) 2049
MspA1I CMGCKG 4 cut(s) 98, 1190, 1454, 4079
MspI CCGG 7 cut(s) 185, 278, 1418, 1781, 3557, 3828, 4028
MunI CAATTG 2 cut(s) 2921, 2948
Mva1269I GAATGC 3 cut(s) 2195, 2700, 3677
MvaI CCWGG 9 cut(s) 250, 258, 1158, 1434, 1765, 2071, 2267, 2451, 2861
NciI CCSGG 2 cut(s) 1419, 3558
NcoI CCATGG 2 cut(s) 3666, 3851
NdeI CATATG 3 cut(s) 799, 2359, 3961
NheI GCTAGC 1 cut(s) 1990
NlaIV GGNNCC 8 cut(s) 243, 254, 262, 1260, 2817, 2857, 2895, 3861
NmeAIII GCCGAG 1 cut(s) 1951
NmuCI GTSAC 4 cut(s) 2171, 3329, 3577, 3731
NsiI ATGCAT 1 cut(s) 2700
NspI RCATGY 4 cut(s) 209, 430, 1877, 4071
NspV TTCGAA 1 cut(s) 422
OliI CACNNNNGTG 1 cut(s) 3978
PagI TCATGA 2 cut(s) 1228, 3657
PasI CCCWGGG 2 cut(s) 1764, 2070
PciI ACATGT 1 cut(s) 426
PciSI GCTCTTC 2 cut(s) 63, 3333
PctI GAATGC 3 cut(s) 2195, 2700, 3677
PdmI GAANNNNTTC 2 cut(s) 3198, 3448
Pfl23II CGTACG 1 cut(s) 2712
PflMI CCANNNNNTGG 3 cut(s) 704, 2501, 3278
PinAI ACCGGT 1 cut(s) 184
PleI GAGTC 4 cut(s) 1651, 2858, 3175, 3642
PpsI GAGTC 4 cut(s) 1651, 2858, 3175, 3642
Ppu21I YACGTR 1 cut(s) 4127
PscI ACATGT 1 cut(s) 426
PsiI TTATAA 2 cut(s) 573, 3320
Psp6I CCWGG 9 cut(s) 248, 256, 1156, 1432, 1763, 2069, 2265, 2449, 2859
PspFI CCCAGC 1 cut(s) 200
PspGI CCWGG 9 cut(s) 248, 256, 1156, 1432, 1763, 2069, 2265, 2449, 2859
PspLI CGTACG 1 cut(s) 2712
PspN4I GGNNCC 8 cut(s) 243, 254, 262, 1260, 2817, 2857, 2895, 3861
PspPI GGNCC 7 cut(s) 260, 404, 1325, 1481, 2024, 2395, 3554
PsrI GAACNNNNNNTAC 2 cut(s) 3355, 3387
PstI CTGCAG 2 cut(s) 1642, 3217
PstNI CAGNNNCTG 3 cut(s) 1157, 1402, 1943
PsuI RGATCY 8 cut(s) 841, 920, 1258, 1370, 2089, 2893, 3022, 3757
PvuII CAGCTG 2 cut(s) 98, 4079
RseI CAYNNNNRTG 9 cut(s) 272, 291, 1014, 1652, 2703, 3519, 3671, 3850, 3978
Rsr2I CGGWCCG 1 cut(s) 3554
RsrII CGGWCCG 1 cut(s) 3554
SapI GCTCTTC 2 cut(s) 63, 3333
Sau96I GGNCC 7 cut(s) 260, 404, 1325, 1481, 2024, 2395, 3554
SchI GAGTC 4 cut(s) 1651, 2858, 3176, 3643
SduI GDGCHC 7 cut(s) 75, 246, 257, 1380, 1647, 2860, 3401
SexAI ACCWGGT 2 cut(s) 248, 2265
SfaNI GCATC 9 cut(s) 295, 838, 1024, 1623, 1633, 1861, 2707, 3028, 3210
SfcI CTRYAG 5 cut(s) 93, 415, 1311, 1638, 3213
SfuI TTCGAA 1 cut(s) 422
SgrAI CRCCGGYG 1 cut(s) 184
SinI GGWCC 3 cut(s) 1481, 2024, 3554
SmiMI CAYNNNNRTG 9 cut(s) 272, 291, 1014, 1652, 2703, 3519, 3671, 3850, 3978
SpeI ACTAGT 1 cut(s) 1235
StyI CCWWGG 7 cut(s) 214, 2050, 2897, 2930, 3301, 3666, 3851
TaiI ACGT 2 cut(s) 2178, 4129
TaqI TCGA 7 cut(s) 55, 103, 422, 915, 3187, 3754, 3906
TatI WGTACW 3 cut(s) 991, 1238, 3712
TauI GCSGC 3 cut(s) 1624, 4018, 4139
TseFI GTSAC 4 cut(s) 2171, 3329, 3577, 3731
Tsp45I GTSAC 4 cut(s) 2171, 3329, 3577, 3731
TspGWI ACGGA 3 cut(s) 1067, 1860, 3567
Van91I CCANNNNNTGG 3 cut(s) 704, 2501, 3278
VneI GTGCAC 1 cut(s) 1643
VpaK11BI GGWCC 3 cut(s) 1481, 2024, 3554
XagI CCTNNNNNAGG 2 cut(s) 1161, 4152
XapI RAATTY 9 cut(s) 16, 1114, 1984, 2281, 2540, 2627, 2763, 3251, 4143
XbaI TCTAGA 1 cut(s) 2521
XceI RCATGY 4 cut(s) 209, 430, 1877, 4071
XcmI CCANNNNNNNNNTGG 2 cut(s) 1592, 2756
XmaJI CCTAGG 3 cut(s) 214, 2930, 3301
XmiI GTMKAC 1 cut(s) 1743
XmnI GAANNNNTTC 2 cut(s) 3198, 3448
Zsp2I ATGCAT 1 cut(s) 2700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.