pycom02g01210

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0004627
Physical Location & Seq
Reverse (-)
687715 .. 692457
4743 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g01210.1

Sequence Viewer

Length: 3735 bp
ATGTTTAATAGGAGTGCTGGCAGTGGTTTAGATCATGGAGTTAATGATATGGCAGCTAGGATTGGTTATAATCCCAATTTAGGCAATAAGGTCAGTGGCAATGTTGCTGATCAGACTGGGAATGATTCGACATCGGGGTATGGTTGTAATGCCAATTTGGGCAATCGTGGCAGCGGGACTGAACAAGCAAGTGATGACGGTGGAGAAGATTCTGTGTCTGGGAAGAAAATAAAGCTTCTGTGCAGCTTTGGCGGAAAGATTTTGCCCAGACCAAGTGATGGAATGTTGAGGTATGTTGGAGGGCAGACAAGGATTATTAGTGTTCGGAGAGATGTGAGCTTCAATGAGCTAGTGCAGAAGATTGTGGATACATTTGGGCAACCTGTGGTCATTAAATACCAGCTCCCCGATGAGGATCTCGATGCACTGGTGTCGGTTTCATGTGCTGATGATCTTGACAATATGAAGGATGAATATGGGAAGTTGGTCGAGAGATCTCCAGATGGTTCAGCAAAGTTGAGGGTATTTTTGTTTTCTGCTTCTGAAGTTGATCCATCTGGTGTCGTACAAATTGGGGATTTACATAACAGTGAGCAGAGATATGTTGATGCTGTCAATGGGATTACGGATGGGGTGAGTGTTGGTATTGTGAGGAAGGAGAGCAATGCAAGTGCAACTTCAACTCAAAATTCTGATTTTAGTGGAACTGATGTTGTTGATAACTCAATTCCTGGTCAGGGGGACAATACTGGACTTCCATCACCGGGCAAGTTATCACCGAAAGGGGATTCAGCTACTTCTCATGATGCTAGTACAAGGCTGGTATTTGTGGATCCTAAGCCTGAAATTTATTCTGAAGTTTCTACACTTCCATTGGTCATTCCTGTGGTTAAGTCTGGGCCTCCCCAAACACCATCTTCTCATCCGGAAGGTGAGTTAGAAAGATCTGTGCCTGTTACAGTATCACAGCAGCAACCTGGAATTGGCATTTCATCTACTGCTCCTTATCTGCAAACTTATGTTGGTCCTCGCCAGGAGGTTATGACACGGGCAGACCATATCCAGCTTCCTCCTCAGATGGGTTTCCCAAGTCCTCACCTATTGGGGACTGCTGGTCCTGTATTCACCAAACAGCAATACGGTGATAGTATTGCTGGCATCACCCCGCATCACTTCATTCCTGCAGCACACATGACAATGACTCCCTCGTCTTCTCATGTTAATATAAGACCAAGTGTGCTTCAGCCATCGATCCATCCACAACAGACTCGGTTGGATAAGTATGCAGGCGAGAGTACCTATGTTCCCAATGTCGTCCAGCTTCCTGCTGAACAAAGCTATAATTCCTATCAGGTTCAAGTACCCTTAACAACAGCAGGAGGTGCTTACGGATGGCATCAAGTTCAACCACCGGAGCATGTGATTTACCATGATGCATTGGTATCTCACCAACAGGTTCAGAGAGACATTGGCGGAAGCCCGGTATCTGATTCAAATCCAACTTATTATAGTCCTCGGCTGGAGGAGAATTTGCGAGCTCAGCCGATGAACAGGGGAGAAGGTAATTTTGGCCAAGGAGTTGAGGCTCGACCTAGGGTTCAAATTCCAGTAGATCCTCGTCTCATAACATCTCATTCAGATGTGACTGGCCTCTCTCAGATTTCATCTCCACAGGGCGTTATCCAGAGAGGAGGTGATGTGCAATCACCAAATGGTGCATTCATGGTCGGTATTCCTCAGCGTTGTCAAGACGATGTTGTTCAACAAAATCCAATGCCATTTCATTACCAGGTTAAACAGGAAAATTTAGGTAACGATCCTTTTAATCAAGATGTACCTCTTGTTGGAGGCGCACCTGTTGAAACTTCAGAATGCCTGGTTCGTGAATCTCCAACTGCATATGATAATCTGAGACAAATCGACGGGAGGATGGAAACACTCAGGATAAATCCCACTGAGCTTTATGTTAATAAGGAGCATGGTGAATCACCTTGGGATATACCTAGAATGGAAGACAACGTTGATCACAAAACACCACAGGTTGGGGGTAGAGAGGTGGCTGTAGATAACATCGTTGACAAATTTGGTAGGAATCACTTTAACTCAACCGAAGTACTAGCTTCTACCCCTGCCGAAGTCTCACATGGGTATAGTTCACAGCCAGCAGAATTCTATGATTTAGGACAACAATCTATATGGGGAAATCCTGAATCTTATCTGCAATCAAGAGTTGGAATATATCCTTGTGATTCTTACCAATCTAATTATGGAACCCCTGTTGTTTCTACCCATCTCACTGATGGAATTCAACCTTTTCCTGAATGGAATGATGAGAATATGAGATTGCAGCCCAAGATGGTACCTAACGAGAGGGAAGTTAATACCTCGAATGGTTTTGGTGCCCAGGACTCCTCAAACTCACTGTTCAGCAATCAAGATCCTTGGAGTTTGTGCCAGGATACTCAATTGCCTCCTAAGCCAACCAAAATACAATTGAGAAAAGAACCATATACAGAAATGCGAATGGACAATGGAGGTCAACAGCCACCAGGCAATTTGAACAGGGATCTTAGTTCAGAGCATGCTCCGTCGTCCAAAGGATCAGCAGAAGAACAAATAAAGCAAGAACTTAAAGCTGTTGCCGAGGGAGTAGCTGCTCGTGTTTTTCAGTCATCTACGCCATCAGATTCTTACTTGCATGATAAAAATGAGTCTGCTTATGAATCCAATCGAGATGAAGAGGTTCAAAACAATGCTGCATGGATGCAAAACAGATCAGAAAGTGAGGATGTCAAATCCAACTTTGCAGATAAGCCAAATCTTGGTTTTCCAGTAGCAGATGGCCTAGGCCGGTTGCAGATTATAAAGAACAGTGACCTTGAAGAGATGAGAGAATTAGGTTCTGGAACTTTTGGTACCGTTTATCATGGAAAATGGAGGGGCACTGATGTTGCAATCAAAAGAATCAATGATAGGTGTTTTTTCGGGAAGCCTTCAGAACAAGACCGCATGAGAGATGATTTCTGGAATGAGGCCATCAAGCTTGCTGACTTGCACCACCCCAATGTGGTAGCTTTCTATGGTGTTGTGCTTGACGGACCCGGAGGTTCTGTGGCAACAGTGACAGAATATATGGTTAACGGCTCTCTGAGAAATGCCTTGCAGAAAAATGAGAAGAGTCTAGACAAGCGCAAGCGCCTCATGATTGCAATGGATGTGGCATTCGGAATGGAGTACCTGCATGAAAAGAAGATAGTACACTTTGATTTGAAGAGTGACAACTTACTTGTCAATCTTCGAGATCCACACCGCCCAATATGCAAGGTCGGTGATTTGGGCCTATCTAAGGTGAAATGTCATACGCTGATCTCCGGTGGTGTGCGGGGAACACTTCCATGGATGGCACCGGAGCTGTTGAGTGGTAGCAGTAGCCTTGTTTCTGAGAAGGTCGATGTGTTTTCTTTCGGTATCGTGTTGTGGGAGCTGCTCACTGGAGACGAACCATATGCGGATTTGCACTATGGTGCTATCATAGGTGGTATTGTGAGCAACACATTGCGGCCACCAATACCGGACTCTTGCGATCCCGAATGGAAATCACTGATGGAGAGATGCTGGTCATCGGAGCCATCCGAGAGGCTGAGTTTCACTGAAATTGCGAACGGATTACGCTCTATGGCAGCAAAGGTTCCTCCTAAAGGACAGACCCAACCGCAGCAACCCTCGGCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04010 map04010
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1245

Amino Acids

136.0

Weight (kDa)

5.19

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 96 - 179 1.6e-16 PB1 domain
PK_Tyr_Ser-Thr PF07714 961 - 1221 1.9e-60 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 961 - 1220 1.3e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 69, 2864
AasI GACNNNNNNGTC 1 cut(s) 1207
Acc36I ACCTGC 1 cut(s) 3246
Acc65I GGTACC 2 cut(s) 2359, 2915
AccB1I GGYRCC 4 cut(s) 2359, 2399, 2915, 3403
AccB7I CCANNNNNTGG 3 cut(s) 278, 2042, 2822
AccIII TCCGGA 1 cut(s) 925
AclI AACGTT 1 cut(s) 2019
AcoI YGGCCR 2 cut(s) 1570, 3560
AcsI RAATTY 8 cut(s) 688, 846, 1528, 1602, 1804, 2081, 2168, 2304
AcuI CTGAAG 5 cut(s) 564, 876, 1226, 1851, 2979
AhdI GACNNNNNGTC 1 cut(s) 1113
AjnI CCWGG 8 cut(s) 730, 976, 1032, 1788, 1875, 2403, 2454, 2548
AjuI GAANNNNNNNTTGG 2 cut(s) 1551, 1583
AleI CACNNNNGTG 1 cut(s) 3522
Alw21I GWGCWC 1 cut(s) 1540
Alw26I GTCTC 5 cut(s) 1458, 1625, 1906, 2143, 3489
Aor13HI TCCGGA 1 cut(s) 925
AoxI GGCC 8 cut(s) 899, 1570, 1648, 2842, 2848, 3033, 3337, 3560
ApoI RAATTY 8 cut(s) 688, 846, 1528, 1602, 1804, 2081, 2168, 2304
Asp700I GAANNNNTTC 1 cut(s) 2742
Asp718I GGTACC 2 cut(s) 2359, 2915
AspA2I CCTAGG 2 cut(s) 1592, 2845
AspLEI GCGC 3 cut(s) 1853, 3192, 3198
AspS9I GGNCC 5 cut(s) 899, 1025, 1115, 3098, 3337
AsuC2I CCSGG 3 cut(s) 765, 1481, 3102
AvaII GGWCC 3 cut(s) 1025, 1115, 3098
AvrII CCTAGG 2 cut(s) 1592, 2845
BaeGI GKGCMC 2 cut(s) 2404, 2945
BalI TGGCCA 1 cut(s) 1572
BamHI GGATCC 1 cut(s) 832
BanI GGYRCC 4 cut(s) 2359, 2399, 2915, 3403
BanII GRGCYC 1 cut(s) 1540
BauI CACGAG 1 cut(s) 2658
BbsI GAAGAC 2 cut(s) 1203, 2019
Bbv12I GWGCWC 1 cut(s) 1540
BbvCI CCTCAGC 1 cut(s) 1737
BceAI ACGGC 1 cut(s) 3157
BcgI CGANNNNNNTGC 4 cut(s) 414, 448, 3488, 3522
BciT130I CCWGG 8 cut(s) 732, 978, 1034, 1790, 1877, 2405, 2456, 2550
BciVI GTATCC 2 cut(s) 361, 2452
BclI TGATCA 2 cut(s) 109, 2023
BcnI CCSGG 3 cut(s) 765, 1481, 3102
BcoDI GTCTC 5 cut(s) 1458, 1625, 1906, 2143, 3489
BfaI CTAG 8 cut(s) 57, 350, 810, 1593, 2004, 2117, 2846, 3182
BfmI CTRYAG 2 cut(s) 1182, 2061
BfoI RGCGCY 1 cut(s) 3199
BfuAI ACCTGC 1 cut(s) 3246
BfuI GTATCC 2 cut(s) 361, 2452
BglII AGATCT 2 cut(s) 494, 944
BlnI CCTAGG 2 cut(s) 1592, 2845
BlpI GCTNAGC 1 cut(s) 1539
BmcAI AGTACT 1 cut(s) 2115
Bme18I GGWCC 3 cut(s) 1025, 1115, 3098
BmeRI GACNNNNNGTC 1 cut(s) 1113
BmgT120I GGNCC 5 cut(s) 899, 1025, 1115, 3098, 3337
BmiI GGNNCC 9 cut(s) 834, 2272, 2361, 2401, 2917, 3100, 3405, 3627, 3690
BmrI ACTGGG 1 cut(s) 126
BmsI GCATC 9 cut(s) 412, 598, 796, 1167, 1177, 1405, 1423, 2754, 3602
BmuI ACTGGG 1 cut(s) 126
BpiI GAAGAC 2 cut(s) 1203, 2019
BpmI CTGGAG 3 cut(s) 483, 1541, 3513
Bpu10I CCTNAGC 3 cut(s) 837, 1737, 2475
Bpu1102I GCTNAGC 1 cut(s) 1539
BpuMI CCSGG 3 cut(s) 765, 1481, 3102
Bsa29I ATCGAT 1 cut(s) 1250
BsaBI GATNNNNATC 2 cut(s) 414, 1494
BsaWI WCCGGW 5 cut(s) 925, 1411, 3371, 3406, 3571
BsaXI ACNNNNNCTCC 6 cut(s) 1186, 1216, 1683, 1713, 3096, 3126
Bse118I RCCGGY 1 cut(s) 2850
Bse1I ACTGG 7 cut(s) 121, 432, 754, 1607, 1651, 2831, 3496
Bse3DI GCAATG 4 cut(s) 106, 670, 3216, 3554
Bse8I GATNNNNATC 2 cut(s) 414, 1494
BseAI TCCGGA 1 cut(s) 925
BseBI CCWGG 8 cut(s) 732, 978, 1034, 1790, 1877, 2405, 2456, 2550
BseCI ATCGAT 1 cut(s) 1250
BseJI GATNNNNATC 2 cut(s) 414, 1494
BseMI GCAATG 4 cut(s) 106, 670, 3216, 3554
BseNI ACTGG 7 cut(s) 121, 432, 754, 1607, 1651, 2831, 3496
BseRI GAGGAG 4 cut(s) 1062, 1538, 1704, 2401
BseSI GKGCMC 2 cut(s) 2404, 2945
BsgI GTGCAG 2 cut(s) 262, 374
BshFI GGCC 8 cut(s) 901, 1572, 1650, 2844, 2850, 3035, 3339, 3562
BshNI GGYRCC 4 cut(s) 2359, 2399, 2915, 3403
BshVI ATCGAT 1 cut(s) 1250
BsiHKAI GWGCWC 1 cut(s) 1540
BsiSI CCGG 9 cut(s) 764, 926, 1412, 1481, 2851, 3102, 3372, 3407, 3572
BslFI GGGAC 3 cut(s) 190, 755, 1120
BsmAI GTCTC 5 cut(s) 1458, 1625, 1906, 2143, 3489
BsmBI CGTCTC 2 cut(s) 1625, 3489
BsmFI GGGAC 3 cut(s) 190, 755, 1120
BsmI GAATGC 3 cut(s) 1718, 1877, 3221
BsnI GGCC 8 cut(s) 901, 1572, 1650, 2844, 2850, 3035, 3339, 3562
Bsp1286I GDGCHC 3 cut(s) 1540, 2404, 2945
Bsp13I TCCGGA 1 cut(s) 925
Bsp1720I GCTNAGC 1 cut(s) 1539
Bsp19I CCATGG 1 cut(s) 3395
BspANI GGCC 8 cut(s) 901, 1572, 1650, 2844, 2850, 3035, 3339, 3562
BspDI ATCGAT 1 cut(s) 1250
BspEI TCCGGA 1 cut(s) 925
BspHI TCATGA 2 cut(s) 802, 3201
BspLI GGNNCC 9 cut(s) 834, 2272, 2361, 2401, 2917, 3100, 3405, 3627, 3690
BspMAI CTGCAG 1 cut(s) 1186
BspMI ACCTGC 1 cut(s) 3246
BspT107I GGYRCC 4 cut(s) 2359, 2399, 2915, 3403
BsrDI GCAATG 4 cut(s) 106, 670, 3216, 3554
BsrFI RCCGGY 1 cut(s) 2850
BsrI ACTGG 7 cut(s) 121, 432, 754, 1607, 1651, 2831, 3496
BssAI RCCGGY 1 cut(s) 2850
BssSI CACGAG 1 cut(s) 2658
BssT1I CCWWGG 6 cut(s) 1573, 1592, 1991, 2441, 2845, 3395
Bst2BI CACGAG 1 cut(s) 2658
Bst2UI CCWGG 8 cut(s) 732, 978, 1034, 1790, 1877, 2405, 2456, 2550
Bst4CI ACNGT 8 cut(s) 200, 590, 961, 1142, 2424, 2873, 2920, 3121
Bst6I CTCTTC 4 cut(s) 2733, 2877, 3170, 3266
BstAPI GCANNNNNTGC 1 cut(s) 1382
BstC8I GCNNGC 8 cut(s) 19, 1156, 1288, 1536, 2163, 2583, 3045, 3194
BstDSI CCRYGG 1 cut(s) 3395
BstENI CCTNNNNNAGG 2 cut(s) 3344, 3696
BstH2I RGCGCY 1 cut(s) 3199
BstHHI GCGC 3 cut(s) 1853, 3192, 3198
BstMAI GTCTC 5 cut(s) 1458, 1625, 1906, 2143, 3489
BstMWI GCNNNNNNNGC 6 cut(s) 168, 249, 1382, 1540, 2476, 3318
BstNI CCWGG 8 cut(s) 732, 978, 1034, 1790, 1877, 2405, 2456, 2550
BstNSI RCATGY 2 cut(s) 1421, 2585
BstSFI CTRYAG 2 cut(s) 1182, 2061
BstSLI GKGCMC 2 cut(s) 2404, 2945
BstV2I GAAGAC 2 cut(s) 1203, 2019
BstX2I RGATCY 8 cut(s) 415, 494, 832, 944, 1612, 2437, 2566, 3301
BstYI RGATCY 8 cut(s) 415, 494, 832, 944, 1612, 2437, 2566, 3301
Bsu15I ATCGAT 1 cut(s) 1250
BsuI GTATCC 2 cut(s) 361, 2452
BsuRI GGCC 8 cut(s) 901, 1572, 1650, 2844, 2850, 3035, 3339, 3562
BsuTUI ATCGAT 1 cut(s) 1250
BtgI CCRYGG 1 cut(s) 3395
BtsI GCAGTG 1 cut(s) 28
BveI ACCTGC 1 cut(s) 3246
Cac8I GCNNGC 8 cut(s) 19, 1156, 1288, 1536, 2163, 2583, 3045, 3194
CciI TCATGA 2 cut(s) 802, 3201
CfoI GCGC 3 cut(s) 1853, 3192, 3198
Cfr10I RCCGGY 1 cut(s) 2850
Cfr13I GGNCC 5 cut(s) 899, 1025, 1115, 3098, 3337
ClaI ATCGAT 1 cut(s) 1250
CsiI ACCWGGT 1 cut(s) 1788
CspCI CAANNNNNGTGG 2 cut(s) 3198, 3233
DrdI GACNNNNNNGTC 1 cut(s) 1207
DriI GACNNNNNGTC 1 cut(s) 1113
DseDI GACNNNNNNGTC 1 cut(s) 1207
EaeI YGGCCR 2 cut(s) 1570, 3560
Eam1104I CTCTTC 4 cut(s) 2733, 2877, 3170, 3266
Eam1105I GACNNNNNGTC 1 cut(s) 1113
EarI CTCTTC 4 cut(s) 2733, 2877, 3170, 3266
EciI GGCGGA 2 cut(s) 267, 1488
Ecl136II GAGCTC 1 cut(s) 1538
Eco130I CCWWGG 6 cut(s) 1573, 1592, 1991, 2441, 2845, 3395
Eco24I GRGCYC 1 cut(s) 1540
Eco47I GGWCC 3 cut(s) 1025, 1115, 3098
Eco53kI GAGCTC 1 cut(s) 1538
Eco57I CTGAAG 5 cut(s) 564, 876, 1226, 1851, 2979
EcoICRI GAGCTC 1 cut(s) 1538
EcoNI CCTNNNNNAGG 2 cut(s) 3344, 3696
EcoRI GAATTC 2 cut(s) 2168, 2304
EcoRII CCWGG 8 cut(s) 730, 976, 1032, 1788, 1875, 2403, 2454, 2548
EcoT14I CCWWGG 6 cut(s) 1573, 1592, 1991, 2441, 2845, 3395
EcoT22I ATGCAT 1 cut(s) 1438
EcoT38I GRGCYC 1 cut(s) 1540
ErhI CCWWGG 6 cut(s) 1573, 1592, 1991, 2441, 2845, 3395
Esp3I CGTCTC 2 cut(s) 1625, 3489
FalI AAGNNNNNCTT 2 cut(s) 661, 693
FaqI GGGAC 3 cut(s) 190, 755, 1120
FauI CCCGC 3 cut(s) 167, 1173, 3375
FauNDI CATATG 2 cut(s) 1900, 3505
FbaI TGATCA 2 cut(s) 109, 2023
FriOI GRGCYC 1 cut(s) 1540
FspBI CTAG 8 cut(s) 57, 350, 810, 1593, 2004, 2117, 2846, 3182
GlaI GCGC 3 cut(s) 1852, 3191, 3197
GsuI CTGGAG 3 cut(s) 483, 1541, 3513
HaeII RGCGCY 1 cut(s) 3199
HaeIII GGCC 8 cut(s) 901, 1572, 1650, 2844, 2850, 3035, 3339, 3562
HapII CCGG 9 cut(s) 764, 926, 1412, 1481, 2851, 3102, 3372, 3407, 3572
HhaI GCGC 3 cut(s) 1853, 3192, 3198
Hin6I GCGC 3 cut(s) 1851, 3190, 3196
HinP1I GCGC 3 cut(s) 1851, 3190, 3196
HincII GTYRAC 3 cut(s) 2077, 2540, 3139
HindII GTYRAC 3 cut(s) 2077, 2540, 3139
HindIII AAGCTT 2 cut(s) 233, 3041
HpaI GTTAAC 1 cut(s) 3139
HpaII CCGG 9 cut(s) 764, 926, 1412, 1481, 2851, 3102, 3372, 3407, 3572
Hpy166II GTNNAC 5 cut(s) 2077, 2156, 2540, 3139, 3259
Hpy8I GTNNAC 5 cut(s) 2077, 2156, 2540, 3139, 3259
Hpy99I CGWCG 2 cut(s) 1925, 2593
HpyAV CCTTC 6 cut(s) 460, 649, 923, 1553, 3003, 3439
HpyCH4III ACNGT 8 cut(s) 200, 590, 961, 1142, 2424, 2873, 2920, 3121
HpyCH4IV ACGT 1 cut(s) 2019
HpyF10VI GCNNNNNNNGC 6 cut(s) 168, 249, 1382, 1540, 2476, 3318
HpySE526I ACGT 1 cut(s) 2019
HspAI GCGC 3 cut(s) 1851, 3190, 3196
Kpn2I TCCGGA 1 cut(s) 925
KpnI GGTACC 2 cut(s) 2363, 2919
Ksp22I TGATCA 2 cut(s) 109, 2023
KspAI GTTAAC 1 cut(s) 3139
LmnI GCTCC 8 cut(s) 408, 1006, 1414, 1975, 2590, 3409, 3481, 3625
LweI GCATC 9 cut(s) 412, 598, 796, 1167, 1177, 1405, 1423, 2754, 3602
MabI ACCWGGT 1 cut(s) 1788
MaeI CTAG 8 cut(s) 57, 350, 810, 1593, 2004, 2117, 2846, 3182
MaeII ACGT 1 cut(s) 2019
MaeIII GTNAC 6 cut(s) 955, 1642, 1811, 2873, 3121, 3275
MfeI CAATTG 2 cut(s) 2465, 2492
MflI RGATCY 8 cut(s) 415, 494, 832, 944, 1612, 2437, 2566, 3301
MhlI GDGCHC 3 cut(s) 1540, 2404, 2945
MlsI TGGCCA 1 cut(s) 1572
MluNI TGGCCA 1 cut(s) 1572
MlyI GAGTC 6 cut(s) 1195, 1261, 2402, 2720, 3187, 3569
MmeI TCCRAC 7 cut(s) 277, 1254, 1523, 1825, 1916, 2212, 2823
Mox20I TGGCCA 1 cut(s) 1572
Mph1103I ATGCAT 1 cut(s) 1438
MroI TCCGGA 1 cut(s) 925
MroXI GAANNNNTTC 1 cut(s) 2742
MscI TGGCCA 1 cut(s) 1572
MslI CAYNNNNRTG 7 cut(s) 588, 884, 1196, 1638, 3063, 3394, 3522
Msp20I TGGCCA 1 cut(s) 1572
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 9 cut(s) 764, 926, 1412, 1481, 2851, 3102, 3372, 3407, 3572
MunI CAATTG 2 cut(s) 2465, 2492
Mva1269I GAATGC 3 cut(s) 1718, 1877, 3221
MvaI CCWGG 8 cut(s) 732, 978, 1034, 1790, 1877, 2405, 2456, 2550
MwoI GCNNNNNNNGC 6 cut(s) 168, 249, 1382, 1540, 2476, 3318
NciI CCSGG 3 cut(s) 765, 1481, 3102
NcoI CCATGG 1 cut(s) 3395
NdeI CATATG 2 cut(s) 1900, 3505
NlaIV GGNNCC 9 cut(s) 834, 2272, 2361, 2401, 2917, 3100, 3405, 3627, 3690
NmeAIII GCCGAG 3 cut(s) 1495, 2668, 3704
NmuCI GTSAC 4 cut(s) 1642, 2873, 3121, 3275
NsiI ATGCAT 1 cut(s) 1438
NspI RCATGY 2 cut(s) 1421, 2585
OliI CACNNNNGTG 1 cut(s) 3522
PaeI GCATGC 1 cut(s) 2585
PagI TCATGA 2 cut(s) 802, 3201
PctI GAATGC 3 cut(s) 1718, 1877, 3221
PdmI GAANNNNTTC 1 cut(s) 2742
PflMI CCANNNNNTGG 3 cut(s) 278, 2042, 2822
PleI GAGTC 6 cut(s) 1195, 1261, 2402, 2719, 3186, 3569
PpsI GAGTC 6 cut(s) 1195, 1261, 2402, 2719, 3186, 3569
PsiI TTATAA 2 cut(s) 69, 2864
Psp124BI GAGCTC 1 cut(s) 1540
Psp1406I AACGTT 1 cut(s) 2019
Psp6I CCWGG 8 cut(s) 730, 976, 1032, 1788, 1875, 2403, 2454, 2548
PspGI CCWGG 8 cut(s) 730, 976, 1032, 1788, 1875, 2403, 2454, 2548
PspN4I GGNNCC 9 cut(s) 834, 2272, 2361, 2401, 2917, 3100, 3405, 3627, 3690
PspPI GGNCC 5 cut(s) 899, 1025, 1115, 3098, 3337
PsrI GAACNNNNNNTAC 2 cut(s) 2899, 2931
PstI CTGCAG 1 cut(s) 1186
PsuI RGATCY 8 cut(s) 415, 494, 832, 944, 1612, 2437, 2566, 3301
RseI CAYNNNNRTG 7 cut(s) 588, 884, 1196, 1638, 3063, 3394, 3522
SacI GAGCTC 1 cut(s) 1540
Sau96I GGNCC 5 cut(s) 899, 1025, 1115, 3098, 3337
ScaI AGTACT 1 cut(s) 2115
SchI GAGTC 6 cut(s) 1195, 1261, 2402, 2720, 3187, 3569
SduI GDGCHC 3 cut(s) 1540, 2404, 2945
SexAI ACCWGGT 1 cut(s) 1788
SfaNI GCATC 9 cut(s) 412, 598, 796, 1167, 1177, 1405, 1423, 2754, 3602
SfcI CTRYAG 2 cut(s) 1182, 2061
SinI GGWCC 3 cut(s) 1025, 1115, 3098
SmiMI CAYNNNNRTG 7 cut(s) 588, 884, 1196, 1638, 3063, 3394, 3522
SphI GCATGC 1 cut(s) 2585
SspMI CTAG 8 cut(s) 57, 350, 810, 1593, 2004, 2117, 2846, 3182
SstI GAGCTC 1 cut(s) 1540
StyI CCWWGG 6 cut(s) 1573, 1592, 1991, 2441, 2845, 3395
TaaI ACNGT 8 cut(s) 200, 590, 961, 1142, 2424, 2873, 2920, 3121
TaiI ACGT 1 cut(s) 2022
TatI WGTACW 3 cut(s) 812, 2113, 3256
TauI GCSGC 1 cut(s) 3562
TseFI GTSAC 4 cut(s) 1642, 2873, 3121, 3275
Tsp45I GTSAC 4 cut(s) 1642, 2873, 3121, 3275
TspGWI ACGGA 5 cut(s) 641, 1404, 2577, 3111, 3678
Van91I CCANNNNNTGG 3 cut(s) 278, 2042, 2822
VpaK11BI GGWCC 3 cut(s) 1025, 1115, 3098
XagI CCTNNNNNAGG 2 cut(s) 3344, 3696
XapI RAATTY 8 cut(s) 688, 846, 1528, 1602, 1804, 2081, 2168, 2304
XbaI TCTAGA 1 cut(s) 3181
XceI RCATGY 2 cut(s) 1421, 2585
XcmI CCANNNNNNNNNTGG 2 cut(s) 2264, 2297
XmaJI CCTAGG 2 cut(s) 1592, 2845
XmnI GAANNNNTTC 1 cut(s) 2742
XspI CTAG 8 cut(s) 57, 350, 810, 1593, 2004, 2117, 2846, 3182
ZrmI AGTACT 1 cut(s) 2115
Zsp2I ATGCAT 1 cut(s) 1438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.