FvH4_1g02570

Hydrophobic seed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014898
Physical Location & Seq
Forward (+)
1441026 .. 1442655
1630 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02570.t1

Sequence Viewer

Length: 756 bp
ATGGAGTCCAGTAAGCTCAATGCTCTCTTCTTCATCTTCATCATCGTCATTTCCTCCGCCGCACCGATTCTCGGTTGCCCTACTTGCGGCAAAAAGAAGCCTCCTCCCAAGCACACCAAGCCTAAACCCCCCAAAGGCCCAATCAAGGTTCCCCCAATCAAGGTTCCACCAGTTACAATACCACCTATCGTCAAACCACCAATCACAATCCCACCCGTCATCCCTCCCGTTGTCAAACCACCAATCACAATCCCACCCGTCATCCCTCCCGTTGTCAAACCACCAATCACAATCCCACCAATCGTAAAACCACCTATCGAAATCCCACCAGTGATTCCCCCAGTTGTGAAACCACCGATCACATTACCACCAGTCGTAATCCCTCCGGTGATTCCCCCGATTGTGAAACCACCAGTGGAGATTCCCCCGATCACCACCAAGCCCCCGAGCAAGGGAACACCGTGCCCGCCACCACCCGGAAAACCAGCTCCTAAGGACACTTGCCCCATCGACACACTGAAGCTCGGAGCCTGCGTGGATCTCCTCGGTGGCTTAGTCCACATTGGGCTCGGTGACCCCGTCGTGAACGAGTGCTGCCCTGTGCTCTCAGGACTTGTGGAACTTGAAGCTGCAGTGTGCTTGTGCACTGCTCTTAAAATCAAGCTTCTCAACCTCAACATCTTCGTCCCTATTGCTCTTCAGCTCCTTGTAACTTGTGGCAAGAGCCCTCCTCCTGGTTTCACCTGCTCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

252

Amino Acids

26.08

Weight (kDa)

9.42

Isoelectric Point (pI)

53.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Extensin_1 PF02095 33 - 166 1.2e-06 Extensin-like protein
LTP_2 PF14368 166 - 249 5.4e-06 Probable lipid transfer
Hydrophob_seed PF14547 167 - 250 3e-27 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 168 - 249 1.6e-12 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G10940 AT2G10940
fragaria_vesca FvH4_1g02570
malus_domestica MD02G1023200.v1.1 MD15G1166100.v1.1
prunus_persica Prupe.7G248900_v2.0.a1 Prupe.7G248900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0087601
rosa_laevigata RLG00000015883
rosa_multiflora Rmu_sc0002299.1_g000028
rosa_rugosa Rorug01G0476900
rosa_samantha Rh2AG030500 Rh2BG030300 Rh2CG030700 Rh2DG030500
rosa_wichuraiana Rw2G002420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 752
Acc36I ACCTGC 1 cut(s) 752
AciI CCGC 4 cut(s) 57, 60, 87, 467
AclWI GGATC 1 cut(s) 546
AcuI CTGAAG 2 cut(s) 539, 683
AfiI CCNNNNNNNGG 9 cut(s) 71, 86, 134, 145, 160, 451, 452, 476, 734
AgsI TTSAA 1 cut(s) 626
AjnI CCWGG 1 cut(s) 733
AluBI AGCT 6 cut(s) 16, 488, 523, 629, 664, 703
AluI AGCT 6 cut(s) 16, 488, 523, 629, 664, 703
Alw21I GWGCWC 2 cut(s) 606, 647
Alw44I GTGCAC 1 cut(s) 643
AlwI GGATC 1 cut(s) 546
Ama87I CYCGRG 1 cut(s) 445
AoxI GGCC 1 cut(s) 136
ApaLI GTGCAC 1 cut(s) 643
ApeKI GCWGC 2 cut(s) 594, 629
AspS9I GGNCC 1 cut(s) 137
AsuC2I CCSGG 1 cut(s) 477
AsuHPI GGTGA 4 cut(s) 400, 424, 584, 733
AvaI CYCGRG 1 cut(s) 445
AxyI CCTNAGG 1 cut(s) 492
BaeGI GKGCMC 2 cut(s) 467, 647
BanII GRGCYC 2 cut(s) 570, 728
Bbv12I GWGCWC 2 cut(s) 606, 647
BbvI GCAGC 2 cut(s) 581, 616
BccI CCATC 1 cut(s) 515
BciT130I CCWGG 1 cut(s) 735
BcnI CCSGG 1 cut(s) 477
BfaI CTAG 1 cut(s) 754
BfmI CTRYAG 1 cut(s) 630
BfuAI ACCTGC 1 cut(s) 752
BisI GCNGC 4 cut(s) 60, 88, 595, 630
BlsI GCNGC 4 cut(s) 61, 89, 596, 631
Bme1390I CCNGG 2 cut(s) 477, 735
BmeT110I CYCGRG 1 cut(s) 445
BmgT120I GGNCC 1 cut(s) 137
BmiI GGNNCC 3 cut(s) 150, 165, 529
BmrFI CCNGG 2 cut(s) 477, 735
BmrI ACTGGG 1 cut(s) 335
BmuI ACTGGG 1 cut(s) 335
BplI GAGNNNNNCTC 2 cut(s) 715, 747
BpuMI CCSGG 1 cut(s) 477
BsaJI CCNNGG 1 cut(s) 544
BsaWI WCCGGW 1 cut(s) 385
BsaXI ACNNNNNCTCC 2 cut(s) 519, 549
Bsc4I CCNNNNNNNGG 9 cut(s) 71, 86, 134, 145, 160, 451, 452, 476, 734
Bse1I ACTGG 6 cut(s) 9, 170, 329, 341, 371, 413
Bse21I CCTNAGG 1 cut(s) 492
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 1 cut(s) 544
BseGI GGATG 2 cut(s) 219, 261
BseLI CCNNNNNNNGG 9 cut(s) 71, 86, 134, 145, 160, 451, 452, 476, 734
BseMII CTCAG 1 cut(s) 621
BseNI ACTGG 6 cut(s) 9, 170, 329, 341, 371, 413
BseRI GAGGAG 3 cut(s) 93, 533, 720
BseSI GKGCMC 2 cut(s) 467, 647
BseXI GCAGC 2 cut(s) 581, 616
BshFI GGCC 1 cut(s) 138
BsiHKAI GWGCWC 2 cut(s) 606, 647
BsiHKCI CYCGRG 1 cut(s) 445
BsiSI CCGG 2 cut(s) 386, 477
BslFI GGGAC 1 cut(s) 671
BslI CCNNNNNNNGG 9 cut(s) 71, 86, 134, 145, 160, 451, 452, 476, 734
BsmFI GGGAC 1 cut(s) 671
BsnI GGCC 1 cut(s) 138
BsoBI CYCGRG 1 cut(s) 445
Bsp1286I GDGCHC 5 cut(s) 467, 570, 606, 647, 728
Bsp143I GATC 3 cut(s) 357, 429, 538
BspACI CCGC 4 cut(s) 57, 60, 87, 467
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 1 cut(s) 620
BspLI GGNNCC 3 cut(s) 150, 165, 529
BspMAI CTGCAG 1 cut(s) 634
BspMI ACCTGC 1 cut(s) 752
BspPI GGATC 1 cut(s) 546
BspQI GCTCTTC 1 cut(s) 702
BsrI ACTGG 6 cut(s) 9, 170, 329, 341, 371, 413
BssECI CCNNGG 1 cut(s) 544
BssMI GATC 3 cut(s) 357, 429, 538
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 1 cut(s) 462
Bst6I CTCTTC 2 cut(s) 32, 702
BstC8I GCNNGC 2 cut(s) 467, 532
BstDEI CTNAG 3 cut(s) 492, 553, 607
BstEII GGTNACC 1 cut(s) 572
BstF5I GGATG 2 cut(s) 219, 261
BstKTI GATC 3 cut(s) 360, 432, 541
BstMBI GATC 3 cut(s) 357, 429, 538
BstMWI GCNNNNNNNGC 2 cut(s) 84, 118
BstNI CCWGG 1 cut(s) 735
BstPI GGTNACC 1 cut(s) 572
BstSCI CCNGG 2 cut(s) 475, 733
BstSFI CTRYAG 1 cut(s) 630
BstSLI GKGCMC 2 cut(s) 467, 647
BstV1I GCAGC 2 cut(s) 581, 616
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
Bsu36I CCTNAGG 1 cut(s) 492
BsuRI GGCC 1 cut(s) 138
BtsCI GGATG 2 cut(s) 219, 261
BtsI GCAGTG 2 cut(s) 639, 645
BtsIMutI CAGTG 5 cut(s) 336, 420, 515, 639, 645
BveI ACCTGC 1 cut(s) 752
Cac8I GCNNGC 2 cut(s) 467, 532
Cfr13I GGNCC 1 cut(s) 137
DdeI CTNAG 3 cut(s) 492, 553, 607
DpnI GATC 3 cut(s) 359, 431, 540
DpnII GATC 3 cut(s) 357, 429, 538
Eam1104I CTCTTC 2 cut(s) 32, 702
EarI CTCTTC 2 cut(s) 32, 702
EciI GGCGGA 1 cut(s) 46
Eco24I GRGCYC 2 cut(s) 570, 728
Eco57I CTGAAG 2 cut(s) 539, 683
Eco81I CCTNAGG 1 cut(s) 492
Eco88I CYCGRG 1 cut(s) 445
Eco91I GGTNACC 1 cut(s) 572
EcoO65I GGTNACC 1 cut(s) 572
EcoRII CCWGG 1 cut(s) 733
EcoT38I GRGCYC 2 cut(s) 570, 728
FaqI GGGAC 1 cut(s) 671
FauI CCCGC 1 cut(s) 474
Fnu4HI GCNGC 4 cut(s) 60, 88, 595, 630
FokI GGATG 2 cut(s) 206, 248
FriOI GRGCYC 2 cut(s) 570, 728
Fsp4HI GCNGC 4 cut(s) 60, 88, 595, 630
FspBI CTAG 1 cut(s) 754
GluI GCNGC 4 cut(s) 60, 88, 595, 630
HaeIII GGCC 1 cut(s) 138
HapII CCGG 2 cut(s) 386, 477
HindIII AAGCTT 1 cut(s) 662
HinfI GANTC 5 cut(s) 5, 67, 334, 391, 421
HpaII CCGG 2 cut(s) 386, 477
HphI GGTGA 4 cut(s) 400, 424, 584, 733
Hpy166II GTNNAC 3 cut(s) 559, 586, 645
Hpy188I TCNGA 1 cut(s) 527
Hpy188III TCNNGA 2 cut(s) 583, 609
Hpy8I GTNNAC 3 cut(s) 559, 586, 645
Hpy99I CGWCG 1 cut(s) 584
HpyCH4III ACNGT 1 cut(s) 462
HpyCH4V TGCA 2 cut(s) 632, 645
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 118
HpyF3I CTNAG 3 cut(s) 492, 553, 607
Kzo9I GATC 3 cut(s) 357, 429, 538
LguI GCTCTTC 1 cut(s) 702
LmnI GCTCC 3 cut(s) 493, 527, 708
Lsp1109I GCAGC 2 cut(s) 581, 616
MaeI CTAG 1 cut(s) 754
MaeIII GTNAC 3 cut(s) 172, 572, 709
MalI GATC 3 cut(s) 359, 431, 540
MboI GATC 3 cut(s) 357, 429, 538
MboII GAAGA 5 cut(s) 19, 22, 28, 673, 689
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 5 cut(s) 467, 570, 606, 647, 728
MlyI GAGTC 1 cut(s) 14
MseI TTAA 1 cut(s) 654
MspI CCGG 2 cut(s) 386, 477
MspR9I CCNGG 2 cut(s) 477, 735
MvaI CCWGG 1 cut(s) 735
MwoI GCNNNNNNNGC 2 cut(s) 84, 118
NciI CCSGG 1 cut(s) 477
NdeII GATC 3 cut(s) 357, 429, 538
NlaIV GGNNCC 3 cut(s) 150, 165, 529
NmuCI GTSAC 1 cut(s) 572
PaqCI CACCTGC 1 cut(s) 752
PciSI GCTCTTC 1 cut(s) 702
PcsI WCGNNNNNNNCGW 2 cut(s) 531, 576
PfeI GAWTC 4 cut(s) 67, 334, 391, 421
PflFI GACNNNGTC 1 cut(s) 578
PkrI GCNGC 4 cut(s) 61, 89, 596, 631
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 733
PspEI GGTNACC 1 cut(s) 572
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 3 cut(s) 150, 165, 529
PspPI GGNCC 1 cut(s) 137
PstI CTGCAG 1 cut(s) 634
PsuI RGATCY 1 cut(s) 538
PsyI GACNNNGTC 1 cut(s) 578
SapI GCTCTTC 1 cut(s) 702
SaqAI TTAA 1 cut(s) 654
SatI GCNGC 4 cut(s) 60, 88, 595, 630
Sau3AI GATC 3 cut(s) 357, 429, 538
Sau96I GGNCC 1 cut(s) 137
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 477, 735
SduI GDGCHC 5 cut(s) 467, 570, 606, 647, 728
SfcI CTRYAG 1 cut(s) 630
SsiI CCGC 4 cut(s) 57, 60, 87, 467
SspMI CTAG 1 cut(s) 754
StyD4I CCNGG 2 cut(s) 475, 733
TaaI ACNGT 1 cut(s) 462
TaqI TCGA 2 cut(s) 318, 510
TauI GCSGC 2 cut(s) 62, 90
TfiI GAWTC 4 cut(s) 67, 334, 391, 421
Tru1I TTAA 1 cut(s) 654
Tru9I TTAA 1 cut(s) 654
TscAI CASTG 5 cut(s) 336, 420, 522, 639, 652
TseFI GTSAC 1 cut(s) 572
TseI GCWGC 2 cut(s) 594, 629
Tsp45I GTSAC 1 cut(s) 572
TspDTI ATGAA 2 cut(s) 22, 28
TspRI CASTG 5 cut(s) 336, 420, 522, 639, 652
Tth111I GACNNNGTC 1 cut(s) 578
VneI GTGCAC 1 cut(s) 643
XspI CTAG 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.