Rh2BG030300

Hydrophobic seed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014898
Physical Location & Seq
Forward (+)
1996619 .. 1998926
2308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG030300.1

Sequence Viewer

Length: 825 bp
ATGGAGTCCAGTAAACTCAATGCTCTCTTCTTCATCTTCATGCTCGTCATTTCCTCGGCCGCTCCGATTCTCGGGTGCCCATCTTGCGGCAAAAAGCCTCCTCCCAAGCACACTAAGCCTAAGACCCCCAAGGGCCCTATTACAGTCCCTCCCATTGTGAAACCCCCAGTCATCAAAGTCCCCCCAGTCACAGTCCCACCCATCGTCAAACCACCAATCACCATCCCCCCCGTCATCCCTCCCATTGTCAAACCACCAATCACAATCCCACCAGTAATCCCTCCCGTCGTAAAACCACCAATCAAAATCCCACCCGTCATCCCTCCCATTGTCAAACCACCAATCACAATCCCACCAGTCATCCCTCCCGTCGTAAAACCACCAATCAAAATCCCACCAGTGATTCCACCAATAGTGAAACCACCAATCACATTACCACCAGTGGTAATCCCTCCAGTGATTCCCCCCATTGTCAAACCACCAGTGGAGATTCCCCCAATCACCACCAAGCCCCCGAGCCCAGGGACACCGTGCCCCCCACCACCTGGGAAACCAGCTCCCAAGGACACTTGCCCCATCGACACACTGAAGCTCGGAGCTTGCGTGGATCTCCTCGGTGGGTTGGTTCACGTTGGGCTCGGTGACCCCGTGGTGAACGAGTGCTGCCCGGTGCTCGCAGGACTTGTTGAACTTGAAGCTGCAGTGTGCTTGTGCACCACCCTTAAACTTAAGCTTCTCAACCTCAACATCTTCGTCCCTATTGCTCTTGAGCTCCTTGTCACTTGTGGCAAGAGCCCTCCTCCTGGTTTCACCTGCTCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

274

Amino Acids

28.43

Weight (kDa)

9.48

Isoelectric Point (pI)

51.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrophob_seed PF14547 190 - 273 3.7e-27 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 191 - 272 6.9e-12 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G10940 AT2G10940
fragaria_vesca FvH4_1g02570
malus_domestica MD02G1023200.v1.1 MD15G1166100.v1.1
prunus_persica Prupe.7G248900_v2.0.a1 Prupe.7G248900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0087601
rosa_laevigata RLG00000015883
rosa_multiflora Rmu_sc0002299.1_g000028
rosa_rugosa Rorug01G0476900
rosa_samantha Rh2AG030500 Rh2BG030300 Rh2CG030700 Rh2DG030500
rosa_wichuraiana Rw2G002420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 821
Acc36I ACCTGC 1 cut(s) 821
AccB1I GGYRCC 1 cut(s) 75
AccB7I CCANNNNNTGG 1 cut(s) 545
AccBSI CCGCTC 1 cut(s) 62
AciI CCGC 2 cut(s) 60, 87
AclWI GGATC 1 cut(s) 615
AcoI YGGCCR 1 cut(s) 57
AcuI CTGAAG 1 cut(s) 608
AfiI CCNNNNNNNGG 5 cut(s) 71, 86, 521, 545, 803
AflII CTTAAG 1 cut(s) 728
AgsI TTSAA 2 cut(s) 689, 695
AjnI CCWGG 3 cut(s) 520, 544, 802
AluBI AGCT 6 cut(s) 557, 592, 599, 698, 733, 772
AluI AGCT 6 cut(s) 557, 592, 599, 698, 733, 772
Alw21I GWGCWC 3 cut(s) 675, 716, 774
Alw44I GTGCAC 1 cut(s) 712
AlwI GGATC 1 cut(s) 615
Ama87I CYCGRG 2 cut(s) 71, 514
AoxI GGCC 2 cut(s) 57, 133
ApaI GGGCCC 1 cut(s) 137
ApaLI GTGCAC 1 cut(s) 712
ApeKI GCWGC 2 cut(s) 663, 698
AspS9I GGNCC 2 cut(s) 133, 134
AsuC2I CCSGG 1 cut(s) 668
AsuHPI GGTGA 5 cut(s) 211, 493, 653, 664, 802
AvaI CYCGRG 2 cut(s) 71, 514
BaeGI GKGCMC 4 cut(s) 80, 137, 536, 716
BanI GGYRCC 1 cut(s) 75
BanII GRGCYC 5 cut(s) 137, 521, 639, 774, 797
Bbv12I GWGCWC 3 cut(s) 675, 716, 774
BbvI GCAGC 2 cut(s) 650, 685
BccI CCATC 4 cut(s) 88, 209, 230, 584
BciT130I CCWGG 3 cut(s) 522, 546, 804
BcnI CCSGG 1 cut(s) 668
BfaI CTAG 1 cut(s) 823
BfmI CTRYAG 1 cut(s) 699
BfrI CTTAAG 1 cut(s) 728
BfuAI ACCTGC 1 cut(s) 821
BisI GCNGC 4 cut(s) 60, 88, 664, 699
BlsI GCNGC 4 cut(s) 61, 89, 665, 700
Bme1390I CCNGG 4 cut(s) 522, 546, 668, 804
BmeT110I CYCGRG 2 cut(s) 71, 514
BmgT120I GGNCC 2 cut(s) 133, 134
BmiI GGNNCC 2 cut(s) 77, 135
BmrFI CCNGG 4 cut(s) 522, 546, 668, 804
BmrI ACTGGG 2 cut(s) 161, 179
BmuI ACTGGG 2 cut(s) 161, 179
BplI GAGNNNNNCTC 2 cut(s) 784, 816
BpmI CTGGAG 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 788
BpuMI CCSGG 1 cut(s) 668
BsaJI CCNNGG 8 cut(s) 54, 129, 520, 521, 545, 561, 613, 648
BsaXI ACNNNNNCTCC 4 cut(s) 133, 163, 588, 618
Bsc4I CCNNNNNNNGG 5 cut(s) 71, 86, 521, 545, 803
Bse1I ACTGG 9 cut(s) 9, 167, 185, 272, 356, 398, 440, 455, 482
BseBI CCWGG 3 cut(s) 522, 546, 804
BseDI CCNNGG 8 cut(s) 54, 129, 520, 521, 545, 561, 613, 648
BseGI GGATG 4 cut(s) 222, 234, 318, 360
BseLI CCNNNNNNNGG 5 cut(s) 71, 86, 521, 545, 803
BseNI ACTGG 9 cut(s) 9, 167, 185, 272, 356, 398, 440, 455, 482
BseRI GAGGAG 3 cut(s) 90, 602, 789
BseSI GKGCMC 4 cut(s) 80, 137, 536, 716
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 2 cut(s) 650, 685
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 59, 135
BshNI GGYRCC 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 3 cut(s) 675, 716, 774
BsiHKCI CYCGRG 2 cut(s) 71, 514
BsiSI CCGG 1 cut(s) 668
BslFI GGGAC 5 cut(s) 131, 164, 179, 538, 740
BslI CCNNNNNNNGG 5 cut(s) 71, 86, 521, 545, 803
BsmFI GGGAC 5 cut(s) 131, 164, 179, 538, 740
BsnI GGCC 2 cut(s) 59, 135
BsoBI CYCGRG 2 cut(s) 71, 514
Bsp120I GGGCCC 1 cut(s) 133
Bsp1286I GDGCHC 9 cut(s) 80, 137, 521, 536, 639, 675, 716, 774, 797
Bsp143I GATC 1 cut(s) 607
BspACI CCGC 2 cut(s) 60, 87
BspANI GGCC 2 cut(s) 59, 135
BspLI GGNNCC 2 cut(s) 77, 135
BspMAI CTGCAG 1 cut(s) 703
BspMI ACCTGC 1 cut(s) 821
BspPI GGATC 1 cut(s) 615
BspT107I GGYRCC 1 cut(s) 75
BspTI CTTAAG 1 cut(s) 728
BsrBI CCGCTC 1 cut(s) 62
BsrI ACTGG 9 cut(s) 9, 167, 185, 272, 356, 398, 440, 455, 482
BssECI CCNNGG 8 cut(s) 54, 129, 520, 521, 545, 561, 613, 648
BssMI GATC 1 cut(s) 607
BssT1I CCWWGG 2 cut(s) 129, 561
Bst2UI CCWGG 3 cut(s) 522, 546, 804
Bst4CI ACNGT 3 cut(s) 145, 193, 531
Bst6I CTCTTC 1 cut(s) 32
BstAFI CTTAAG 1 cut(s) 728
BstC8I GCNNGC 2 cut(s) 601, 675
BstDEI CTNAG 2 cut(s) 114, 120
BstDSI CCRYGG 1 cut(s) 648
BstEII GGTNACC 1 cut(s) 641
BstF5I GGATG 4 cut(s) 222, 234, 318, 360
BstKTI GATC 1 cut(s) 610
BstMBI GATC 1 cut(s) 607
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 2 cut(s) 84, 115
BstNI CCWGG 3 cut(s) 522, 546, 804
BstPI GGTNACC 1 cut(s) 641
BstSCI CCNGG 4 cut(s) 520, 544, 666, 802
BstSFI CTRYAG 1 cut(s) 699
BstSLI GKGCMC 4 cut(s) 80, 137, 536, 716
BstV1I GCAGC 2 cut(s) 650, 685
BstX2I RGATCY 1 cut(s) 607
BstYI RGATCY 1 cut(s) 607
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 59, 135
BtgI CCRYGG 1 cut(s) 648
BtsCI GGATG 4 cut(s) 222, 234, 318, 360
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 6 cut(s) 405, 447, 462, 489, 584, 708
BveI ACCTGC 1 cut(s) 821
Cac8I GCNNGC 2 cut(s) 601, 675
Cfr13I GGNCC 2 cut(s) 133, 134
CviAII CATG 1 cut(s) 40
DdeI CTNAG 2 cut(s) 114, 120
DpnI GATC 1 cut(s) 609
DpnII GATC 1 cut(s) 607
EaeI YGGCCR 1 cut(s) 57
EagI CGGCCG 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 772
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 2 cut(s) 129, 561
Eco24I GRGCYC 5 cut(s) 137, 521, 639, 774, 797
Eco52I CGGCCG 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 772
Eco57I CTGAAG 1 cut(s) 608
Eco88I CYCGRG 2 cut(s) 71, 514
Eco91I GGTNACC 1 cut(s) 641
EcoICRI GAGCTC 1 cut(s) 772
EcoO109I RGGNCCY 2 cut(s) 133, 134
EcoO65I GGTNACC 1 cut(s) 641
EcoRII CCWGG 3 cut(s) 520, 544, 802
EcoT14I CCWWGG 2 cut(s) 129, 561
EcoT38I GRGCYC 5 cut(s) 137, 521, 639, 774, 797
ErhI CCWWGG 2 cut(s) 129, 561
FaeI CATG 1 cut(s) 43
FaiI YATR 1 cut(s) 41
FaqI GGGAC 5 cut(s) 131, 164, 179, 538, 740
FatI CATG 1 cut(s) 39
Fnu4HI GCNGC 4 cut(s) 60, 88, 664, 699
FokI GGATG 4 cut(s) 209, 221, 305, 347
FriOI GRGCYC 5 cut(s) 137, 521, 639, 774, 797
Fsp4HI GCNGC 4 cut(s) 60, 88, 664, 699
FspBI CTAG 1 cut(s) 823
GluI GCNGC 4 cut(s) 60, 88, 664, 699
GsuI CTGGAG 1 cut(s) 438
HaeIII GGCC 2 cut(s) 59, 135
HapII CCGG 1 cut(s) 668
Hin1II CATG 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 731
HinfI GANTC 5 cut(s) 5, 67, 403, 460, 490
HpaII CCGG 1 cut(s) 668
HphI GGTGA 5 cut(s) 211, 493, 653, 664, 802
Hpy166II GTNNAC 4 cut(s) 14, 628, 655, 714
Hpy188I TCNGA 2 cut(s) 66, 596
Hpy188III TCNNGA 1 cut(s) 767
Hpy8I GTNNAC 4 cut(s) 14, 628, 655, 714
Hpy99I CGWCG 2 cut(s) 290, 374
HpyCH4III ACNGT 3 cut(s) 145, 193, 531
HpyCH4IV ACGT 1 cut(s) 630
HpyCH4V TGCA 2 cut(s) 701, 714
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 115
HpyF3I CTNAG 2 cut(s) 114, 120
HpySE526I ACGT 1 cut(s) 630
Hsp92II CATG 1 cut(s) 43
Kzo9I GATC 1 cut(s) 607
LmnI GCTCC 4 cut(s) 67, 562, 596, 777
Lsp1109I GCAGC 2 cut(s) 650, 685
MaeI CTAG 1 cut(s) 823
MaeII ACGT 1 cut(s) 630
MaeIII GTNAC 3 cut(s) 187, 641, 778
MalI GATC 1 cut(s) 609
MbiI CCGCTC 1 cut(s) 62
MboI GATC 1 cut(s) 607
MboII GAAGA 4 cut(s) 19, 22, 28, 742
MflI RGATCY 1 cut(s) 607
MhlI GDGCHC 9 cut(s) 80, 137, 521, 536, 639, 675, 716, 774, 797
MlyI GAGTC 1 cut(s) 14
MseI TTAA 2 cut(s) 723, 729
MslI CAYNNNNRTG 1 cut(s) 38
MspCI CTTAAG 1 cut(s) 728
MspI CCGG 1 cut(s) 668
MspR9I CCNGG 4 cut(s) 522, 546, 668, 804
MvaI CCWGG 3 cut(s) 522, 546, 804
MwoI GCNNNNNNNGC 2 cut(s) 84, 115
NciI CCSGG 1 cut(s) 668
NdeII GATC 1 cut(s) 607
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 2 cut(s) 77, 135
NmeAIII GCCGAG 1 cut(s) 35
NmuCI GTSAC 3 cut(s) 187, 641, 778
PaqCI CACCTGC 1 cut(s) 821
PasI CCCWGGG 1 cut(s) 521
PcsI WCGNNNNNNNCGW 3 cut(s) 62, 600, 645
PfeI GAWTC 4 cut(s) 67, 403, 460, 490
PflMI CCANNNNNTGG 1 cut(s) 545
PkrI GCNGC 4 cut(s) 61, 89, 665, 700
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 774
Psp6I CCWGG 3 cut(s) 520, 544, 802
PspEI GGTNACC 1 cut(s) 641
PspGI CCWGG 3 cut(s) 520, 544, 802
PspN4I GGNNCC 2 cut(s) 77, 135
PspOMI GGGCCC 1 cut(s) 133
PspPI GGNCC 2 cut(s) 133, 134
PstI CTGCAG 1 cut(s) 703
PsuI RGATCY 1 cut(s) 607
RseI CAYNNNNRTG 1 cut(s) 38
SacI GAGCTC 1 cut(s) 774
SaqAI TTAA 2 cut(s) 723, 729
SatI GCNGC 4 cut(s) 60, 88, 664, 699
Sau3AI GATC 1 cut(s) 607
Sau96I GGNCC 2 cut(s) 133, 134
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 4 cut(s) 522, 546, 668, 804
SduI GDGCHC 9 cut(s) 80, 137, 521, 536, 639, 675, 716, 774, 797
SfcI CTRYAG 1 cut(s) 699
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 728, 767
SmoI CTYRAG 2 cut(s) 728, 767
SsiI CCGC 2 cut(s) 60, 87
SspMI CTAG 1 cut(s) 823
SstI GAGCTC 1 cut(s) 774
StyD4I CCNGG 4 cut(s) 520, 544, 666, 802
StyI CCWWGG 2 cut(s) 129, 561
TaaI ACNGT 3 cut(s) 145, 193, 531
TaiI ACGT 1 cut(s) 633
TaqI TCGA 1 cut(s) 579
TauI GCSGC 2 cut(s) 62, 90
TfiI GAWTC 4 cut(s) 67, 403, 460, 490
Tru1I TTAA 2 cut(s) 723, 729
Tru9I TTAA 2 cut(s) 723, 729
TscAI CASTG 6 cut(s) 405, 447, 462, 489, 591, 708
TseFI GTSAC 3 cut(s) 187, 641, 778
TseI GCWGC 2 cut(s) 663, 698
Tsp45I GTSAC 3 cut(s) 187, 641, 778
TspDTI ATGAA 2 cut(s) 22, 28
TspRI CASTG 6 cut(s) 405, 447, 462, 489, 591, 708
Van91I CCANNNNNTGG 1 cut(s) 545
Vha464I CTTAAG 1 cut(s) 728
VneI GTGCAC 1 cut(s) 712
XspI CTAG 1 cut(s) 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.