Rmu_sc0002299.1_g000028

Hydrophobic seed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002299.1
OG0014898
Physical Location & Seq
Forward (+)
134301 .. 135649
1349 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002299.1_g000028.1.cds

Sequence Viewer

Length: 873 bp
atggagtccagtaaactcaatgctctcttcttcatcttcatgctcgtcatttcctcggccgctccgattctcgggtgccctacttgcggcaaaaagcctcctcccaagcacactaagcctaagacccccaagggccctattacagtccctcccattgtgaaacccccagtcatcaaagtccccccagtcacagtcccacctatcaaaccaccagtgacaataccacccatcgtcaaaccaccaatcaccatcccccccgtcatccctcccattgtcaaaccaccaatcaccatcccccccgtcatccctcccattgtcaaaccaccaatcacaatcccaccagtaatccctcccgtcgtaaaaccaccaattaaaatcccacccgtcatccctcccattctcaaaccaccaatcacaatcccaccagtcatccctcccgtcataaaaccaccaatcaaaatcccaccagtgattccaccaatagtgaaaccgccaatcacattaccaccagtggtaatccctccagtgattccccccattgtcaaaccaccagtggagattcccccaatcaccaccaagcccccgagccccgggacaccgtgccccccaccacctgggacaccagctcccaaggacacttgccccatcgacacactgaagctcggagcttgcgtggatctcctcggtgggttggttcacgttggggaaggtgaccccgtgtgtgcttgtgcaccacccttaaactcaagcttctcaacctcaacatcttcgtccctattgctcttgagctccttgtcacttgtggcaagagccctcctcctggtttcacctgctctatctagatcaagtcgtggtagattcatggagaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

290

Amino Acids

30.08

Weight (kDa)

10.01

Isoelectric Point (pI)

53.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G10940 AT2G10940
fragaria_vesca FvH4_1g02570
malus_domestica MD02G1023200.v1.1 MD15G1166100.v1.1
prunus_persica Prupe.7G248900_v2.0.a1 Prupe.7G248900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0087601
rosa_laevigata RLG00000015883
rosa_multiflora Rmu_sc0002299.1_g000028
rosa_rugosa Rorug01G0476900
rosa_samantha Rh2AG030500 Rh2BG030300 Rh2CG030700 Rh2DG030500
rosa_wichuraiana Rw2G002420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 838
Acc36I ACCTGC 1 cut(s) 838
AccB1I GGYRCC 1 cut(s) 75
AccB7I CCANNNNNTGG 1 cut(s) 614
AccBSI CCGCTC 1 cut(s) 62
AciI CCGC 3 cut(s) 60, 87, 491
AclWI GGATC 1 cut(s) 684
AcoI YGGCCR 1 cut(s) 57
AcuI CTGAAG 1 cut(s) 677
AfiI CCNNNNNNNGG 5 cut(s) 71, 86, 590, 614, 820
AjnI CCWGG 2 cut(s) 613, 819
AluBI AGCT 5 cut(s) 626, 661, 668, 750, 789
AluI AGCT 5 cut(s) 626, 661, 668, 750, 789
Alw21I GWGCWC 2 cut(s) 733, 791
Alw44I GTGCAC 1 cut(s) 729
AlwI GGATC 1 cut(s) 684
Ama87I CYCGRG 3 cut(s) 71, 583, 590
AoxI GGCC 2 cut(s) 57, 133
ApaI GGGCCC 1 cut(s) 137
ApaLI GTGCAC 1 cut(s) 729
AspS9I GGNCC 2 cut(s) 133, 134
AsuC2I CCSGG 2 cut(s) 591, 592
AsuHPI GGTGA 5 cut(s) 238, 280, 562, 722, 819
AvaI CYCGRG 3 cut(s) 71, 583, 590
BaeGI GKGCMC 4 cut(s) 80, 137, 605, 733
BanI GGYRCC 1 cut(s) 75
BanII GRGCYC 4 cut(s) 137, 590, 791, 814
Bbv12I GWGCWC 2 cut(s) 733, 791
BccI CCATC 4 cut(s) 236, 257, 299, 653
BciT130I CCWGG 2 cut(s) 615, 821
BcnI CCSGG 2 cut(s) 591, 592
BfaI CTAG 1 cut(s) 840
BfuAI ACCTGC 1 cut(s) 838
BisI GCNGC 2 cut(s) 60, 88
BlsI GCNGC 2 cut(s) 61, 89
Bme1390I CCNGG 4 cut(s) 591, 592, 615, 821
BmeT110I CYCGRG 3 cut(s) 71, 583, 590
BmgT120I GGNCC 2 cut(s) 133, 134
BmiI GGNNCC 2 cut(s) 77, 135
BmrFI CCNGG 4 cut(s) 591, 592, 615, 821
BmrI ACTGGG 2 cut(s) 161, 179
BmuI ACTGGG 2 cut(s) 161, 179
BplI GAGNNNNNCTC 2 cut(s) 801, 833
BpmI CTGGAG 1 cut(s) 507
BpuEI CTTGAG 2 cut(s) 730, 805
BpuMI CCSGG 2 cut(s) 591, 592
BsaJI CCNNGG 7 cut(s) 54, 129, 589, 590, 614, 630, 682
BsaXI ACNNNNNCTCC 6 cut(s) 133, 163, 610, 640, 657, 687
Bsc4I CCNNNNNNNGG 5 cut(s) 71, 86, 590, 614, 820
BseBI CCWGG 2 cut(s) 615, 821
BseDI CCNNGG 7 cut(s) 54, 129, 589, 590, 614, 630, 682
BseGI GGATG 6 cut(s) 249, 261, 291, 303, 387, 429
BseLI CCNNNNNNNGG 5 cut(s) 71, 86, 590, 614, 820
BseRI GAGGAG 3 cut(s) 90, 671, 806
BseSI GKGCMC 4 cut(s) 80, 137, 605, 733
BseX3I CGGCCG 1 cut(s) 57
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 59, 135
BshNI GGYRCC 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 2 cut(s) 733, 791
BsiHKCI CYCGRG 3 cut(s) 71, 583, 590
BsiSI CCGG 1 cut(s) 591
BslFI GGGAC 6 cut(s) 131, 164, 179, 607, 631, 757
BslI CCNNNNNNNGG 5 cut(s) 71, 86, 590, 614, 820
BsmFI GGGAC 6 cut(s) 131, 164, 179, 607, 631, 757
BsnI GGCC 2 cut(s) 59, 135
BsoBI CYCGRG 3 cut(s) 71, 583, 590
Bsp120I GGGCCC 1 cut(s) 133
Bsp1286I GDGCHC 7 cut(s) 80, 137, 590, 605, 733, 791, 814
Bsp143I GATC 2 cut(s) 676, 842
BspACI CCGC 3 cut(s) 60, 87, 491
BspANI GGCC 2 cut(s) 59, 135
BspLI GGNNCC 2 cut(s) 77, 135
BspMI ACCTGC 1 cut(s) 838
BspPI GGATC 1 cut(s) 684
BspT107I GGYRCC 1 cut(s) 75
BsrBI CCGCTC 1 cut(s) 62
BssECI CCNNGG 7 cut(s) 54, 129, 589, 590, 614, 630, 682
BssMI GATC 2 cut(s) 676, 842
BssT1I CCWWGG 2 cut(s) 129, 630
Bst2UI CCWGG 2 cut(s) 615, 821
Bst4CI ACNGT 3 cut(s) 145, 193, 600
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 670
BstDEI CTNAG 2 cut(s) 114, 120
BstEII GGTNACC 1 cut(s) 710
BstF5I GGATG 6 cut(s) 249, 261, 291, 303, 387, 429
BstKTI GATC 2 cut(s) 679, 845
BstMBI GATC 2 cut(s) 676, 842
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 2 cut(s) 84, 115
BstNI CCWGG 2 cut(s) 615, 821
BstPI GGTNACC 1 cut(s) 710
BstSCI CCNGG 4 cut(s) 589, 590, 613, 819
BstSLI GKGCMC 4 cut(s) 80, 137, 605, 733
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 59, 135
BtsCI GGATG 6 cut(s) 249, 261, 291, 303, 387, 429
BtsIMutI CAGTG 6 cut(s) 219, 474, 516, 531, 558, 653
BveI ACCTGC 1 cut(s) 838
Cac8I GCNNGC 1 cut(s) 670
Cfr13I GGNCC 2 cut(s) 133, 134
Cfr9I CCCGGG 1 cut(s) 590
CviAII CATG 2 cut(s) 40, 862
DdeI CTNAG 2 cut(s) 114, 120
DpnI GATC 2 cut(s) 678, 844
DpnII GATC 2 cut(s) 676, 842
EaeI YGGCCR 1 cut(s) 57
EagI CGGCCG 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 789
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 2 cut(s) 129, 630
Eco24I GRGCYC 4 cut(s) 137, 590, 791, 814
Eco52I CGGCCG 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 789
Eco57I CTGAAG 1 cut(s) 677
Eco88I CYCGRG 3 cut(s) 71, 583, 590
Eco91I GGTNACC 1 cut(s) 710
EcoICRI GAGCTC 1 cut(s) 789
EcoO109I RGGNCCY 2 cut(s) 133, 134
EcoO65I GGTNACC 1 cut(s) 710
EcoRII CCWGG 2 cut(s) 613, 819
EcoT14I CCWWGG 2 cut(s) 129, 630
EcoT38I GRGCYC 4 cut(s) 137, 590, 791, 814
ErhI CCWWGG 2 cut(s) 129, 630
FaeI CATG 2 cut(s) 43, 865
FaiI YATR 3 cut(s) 41, 443, 863
FaqI GGGAC 6 cut(s) 131, 164, 179, 607, 631, 757
FatI CATG 2 cut(s) 39, 861
Fnu4HI GCNGC 2 cut(s) 60, 88
FokI GGATG 6 cut(s) 236, 248, 278, 290, 374, 416
FriOI GRGCYC 4 cut(s) 137, 590, 791, 814
Fsp4HI GCNGC 2 cut(s) 60, 88
FspBI CTAG 1 cut(s) 840
GluI GCNGC 2 cut(s) 60, 88
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 2 cut(s) 59, 135
HapII CCGG 1 cut(s) 591
Hin1II CATG 2 cut(s) 43, 865
HindIII AAGCTT 1 cut(s) 748
HinfI GANTC 6 cut(s) 5, 67, 472, 529, 559, 858
HpaII CCGG 1 cut(s) 591
HphI GGTGA 5 cut(s) 238, 280, 562, 722, 819
Hpy166II GTNNAC 3 cut(s) 14, 697, 731
Hpy188I TCNGA 2 cut(s) 66, 665
Hpy188III TCNNGA 2 cut(s) 784, 840
Hpy8I GTNNAC 3 cut(s) 14, 697, 731
Hpy99I CGWCG 1 cut(s) 359
HpyAV CCTTC 1 cut(s) 701
HpyCH4III ACNGT 3 cut(s) 145, 193, 600
HpyCH4IV ACGT 1 cut(s) 699
HpyCH4V TGCA 1 cut(s) 731
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 115
HpyF3I CTNAG 2 cut(s) 114, 120
HpySE526I ACGT 1 cut(s) 699
Hsp92II CATG 2 cut(s) 43, 865
Kzo9I GATC 2 cut(s) 676, 842
LmnI GCTCC 4 cut(s) 67, 631, 665, 794
MaeI CTAG 1 cut(s) 840
MaeII ACGT 1 cut(s) 699
MaeIII GTNAC 4 cut(s) 187, 214, 710, 795
MalI GATC 2 cut(s) 678, 844
MbiI CCGCTC 1 cut(s) 62
MboI GATC 2 cut(s) 676, 842
MboII GAAGA 4 cut(s) 19, 22, 28, 759
MflI RGATCY 1 cut(s) 676
MhlI GDGCHC 7 cut(s) 80, 137, 590, 605, 733, 791, 814
MluCI AATT 1 cut(s) 369
MlyI GAGTC 1 cut(s) 14
MseI TTAA 2 cut(s) 372, 740
MslI CAYNNNNRTG 1 cut(s) 38
MspI CCGG 1 cut(s) 591
MspR9I CCNGG 4 cut(s) 591, 592, 615, 821
MvaI CCWGG 2 cut(s) 615, 821
MwoI GCNNNNNNNGC 2 cut(s) 84, 115
NciI CCSGG 2 cut(s) 591, 592
NdeII GATC 2 cut(s) 676, 842
NlaIII CATG 2 cut(s) 43, 865
NlaIV GGNNCC 2 cut(s) 77, 135
NmeAIII GCCGAG 1 cut(s) 35
NmuCI GTSAC 4 cut(s) 187, 214, 710, 795
PaqCI CACCTGC 1 cut(s) 838
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 669
PfeI GAWTC 5 cut(s) 67, 472, 529, 559, 858
PflMI CCANNNNNTGG 1 cut(s) 614
PkrI GCNGC 2 cut(s) 61, 89
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 791
Psp6I CCWGG 2 cut(s) 613, 819
PspEI GGTNACC 1 cut(s) 710
PspGI CCWGG 2 cut(s) 613, 819
PspN4I GGNNCC 2 cut(s) 77, 135
PspOMI GGGCCC 1 cut(s) 133
PspPI GGNCC 2 cut(s) 133, 134
PsuI RGATCY 1 cut(s) 676
RseI CAYNNNNRTG 1 cut(s) 38
SacI GAGCTC 1 cut(s) 791
SaqAI TTAA 2 cut(s) 372, 740
SatI GCNGC 2 cut(s) 60, 88
Sau3AI GATC 2 cut(s) 676, 842
Sau96I GGNCC 2 cut(s) 133, 134
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 4 cut(s) 591, 592, 615, 821
SduI GDGCHC 7 cut(s) 80, 137, 590, 605, 733, 791, 814
SmaI CCCGGG 1 cut(s) 592
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 745, 784
SmoI CTYRAG 2 cut(s) 745, 784
Sse9I AATT 1 cut(s) 369
SsiI CCGC 3 cut(s) 60, 87, 491
SspMI CTAG 1 cut(s) 840
SstI GAGCTC 1 cut(s) 791
StyD4I CCNGG 4 cut(s) 589, 590, 613, 819
StyI CCWWGG 2 cut(s) 129, 630
TaaI ACNGT 3 cut(s) 145, 193, 600
TaiI ACGT 1 cut(s) 702
TaqI TCGA 1 cut(s) 648
TasI AATT 1 cut(s) 369
TauI GCSGC 2 cut(s) 62, 90
TfiI GAWTC 5 cut(s) 67, 472, 529, 559, 858
Tru1I TTAA 2 cut(s) 372, 740
Tru9I TTAA 2 cut(s) 372, 740
TscAI CASTG 6 cut(s) 219, 474, 516, 531, 558, 660
TseFI GTSAC 4 cut(s) 187, 214, 710, 795
Tsp45I GTSAC 4 cut(s) 187, 214, 710, 795
TspDTI ATGAA 3 cut(s) 22, 28, 850
TspMI CCCGGG 1 cut(s) 590
TspRI CASTG 6 cut(s) 219, 474, 516, 531, 558, 660
Van91I CCANNNNNTGG 1 cut(s) 614
VneI GTGCAC 1 cut(s) 729
XbaI TCTAGA 1 cut(s) 839
XmaI CCCGGG 1 cut(s) 590
XspI CTAG 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.