RLG00000015883

Hydrophobic seed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014898
Physical Location & Seq
Forward (+)
2067039 .. 2067890
852 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015883

Sequence Viewer

Length: 852 bp
ATGGAGTCCAGTAAACTCAATGCTCTCTTCTTCATCTTCATGCTCGTCATTTCCTCGGCGGCTCCGATTCTCGGGTGCCCTACCTGCGGCAAAAAGCCTCCTCCCAAGCACACTAAGCCTAAGACCCCCAAGGGCCCTGTTACAGTCCCTCCCATTGTGAAACCCCCCGTCGTCAAAGTCCCCCCAGTCACAGTCCCACCTATCAAACCACCAGTGACAATACCACCCATCGTCAAACCACCAATCACAATCCCCCCCGTCATCCCTCCCGTCGTAAAACCACCAATCAAAATCCCACCAGTAATCCCTCCCGTCGTAAAACCACCAATTAAAATCCCACCCGTCATCCCTCCCATTGTCAAACCACCAATCACAATCCCACCAGTCATCCCTCCCGTCGTAAAACCACCAATCAAAATCCCACCAGTGATTCCACCAATAGTGAAACCACCAGTCACATTACCACCAGTGGTAATCCCTCCAGTGATTCCCCCCATTGTCAAACCACCAGTGGAGATTCCTCCAATCACCACCAAGCCCCCGAGCCCCGGGACACCGTGCCCCCCACCACCTGGGAAACCAGCTTCCAACGACACTTGCCCCATTGACACACTGAAGCTCGGAGCTTGCGTGGATCTCCTCGGTGGGTTGGTTCACGTTGGGCTCGGTGACCCCGTGGTGAACGAGTGCTGCCCGGTGCTCGCAGGACTTGTTGAACTTGAAGCTGCAGTGTGCTTGTGCACCACCCTTAAACTCAAGCTTCTCAACCTCAACATCTTCGTCCCTATTGCTCTTGAGCTCCTTGTCACTTGTGGCAAGAGCCCTCCTCCTGGTTTCACCTGCTCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrophob_seed

Protein Analysis

284

Amino Acids

29.34

Weight (kDa)

9.53

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 194 - 281 9.8e-06 Probable lipid transfer
Hydrophob_seed PF14547 199 - 282 3.9e-27 Hydrophobic seed protein
Tryp_alpha_amyl PF00234 200 - 281 7.3e-12 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G10940 AT2G10940
fragaria_vesca FvH4_1g02570
malus_domestica MD02G1023200.v1.1 MD15G1166100.v1.1
prunus_persica Prupe.7G248900_v2.0.a1 Prupe.7G248900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0087601
rosa_laevigata RLG00000015883
rosa_multiflora Rmu_sc0002299.1_g000028
rosa_rugosa Rorug01G0476900
rosa_samantha Rh2AG030500 Rh2BG030300 Rh2CG030700 Rh2DG030500
rosa_wichuraiana Rw2G002420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 848
Acc36I ACCTGC 2 cut(s) 92, 848
AccB1I GGYRCC 1 cut(s) 75
AccB7I CCANNNNNTGG 1 cut(s) 572
AciI CCGC 2 cut(s) 59, 87
AclWI GGATC 1 cut(s) 642
AcuI CTGAAG 1 cut(s) 635
AfiI CCNNNNNNNGG 5 cut(s) 71, 86, 548, 572, 830
AgsI TTSAA 2 cut(s) 716, 722
AjnI CCWGG 2 cut(s) 571, 829
AluBI AGCT 6 cut(s) 584, 619, 626, 725, 760, 799
AluI AGCT 6 cut(s) 584, 619, 626, 725, 760, 799
Alw21I GWGCWC 3 cut(s) 702, 743, 801
Alw44I GTGCAC 1 cut(s) 739
AlwI GGATC 1 cut(s) 642
Ama87I CYCGRG 3 cut(s) 71, 541, 548
AoxI GGCC 1 cut(s) 133
ApaI GGGCCC 1 cut(s) 137
ApaLI GTGCAC 1 cut(s) 739
ApeKI GCWGC 2 cut(s) 690, 725
AspS9I GGNCC 2 cut(s) 133, 134
AsuC2I CCSGG 3 cut(s) 549, 550, 695
AsuHPI GGTGA 4 cut(s) 520, 680, 691, 829
AvaI CYCGRG 3 cut(s) 71, 541, 548
BaeGI GKGCMC 4 cut(s) 80, 137, 563, 743
BanI GGYRCC 1 cut(s) 75
BanII GRGCYC 5 cut(s) 137, 548, 666, 801, 824
Bbv12I GWGCWC 3 cut(s) 702, 743, 801
BbvI GCAGC 2 cut(s) 677, 712
BccI CCATC 1 cut(s) 236
BciT130I CCWGG 2 cut(s) 573, 831
BcnI CCSGG 3 cut(s) 549, 550, 695
BfaI CTAG 1 cut(s) 850
BfmI CTRYAG 1 cut(s) 726
BfuAI ACCTGC 2 cut(s) 92, 848
BisI GCNGC 4 cut(s) 60, 88, 691, 726
BlsI GCNGC 4 cut(s) 61, 89, 692, 727
Bme1390I CCNGG 5 cut(s) 549, 550, 573, 695, 831
BmeT110I CYCGRG 3 cut(s) 71, 541, 548
BmgT120I GGNCC 2 cut(s) 133, 134
BmiI GGNNCC 3 cut(s) 63, 77, 135
BmrFI CCNGG 5 cut(s) 549, 550, 573, 695, 831
BmrI ACTGGG 1 cut(s) 179
BmuI ACTGGG 1 cut(s) 179
BplI GAGNNNNNCTC 2 cut(s) 811, 843
BpmI CTGGAG 1 cut(s) 465
BpuEI CTTGAG 2 cut(s) 740, 815
BpuMI CCSGG 3 cut(s) 549, 550, 695
BsaJI CCNNGG 7 cut(s) 54, 129, 547, 548, 572, 640, 675
BsaXI ACNNNNNCTCC 4 cut(s) 133, 163, 615, 645
Bsc4I CCNNNNNNNGG 5 cut(s) 71, 86, 548, 572, 830
BseBI CCWGG 2 cut(s) 573, 831
BseDI CCNNGG 7 cut(s) 54, 129, 547, 548, 572, 640, 675
BseGI GGATG 3 cut(s) 261, 345, 387
BseLI CCNNNNNNNGG 5 cut(s) 71, 86, 548, 572, 830
BseRI GAGGAG 3 cut(s) 90, 629, 816
BseSI GKGCMC 4 cut(s) 80, 137, 563, 743
BseXI GCAGC 2 cut(s) 677, 712
BshFI GGCC 1 cut(s) 135
BshNI GGYRCC 1 cut(s) 75
BsiHKAI GWGCWC 3 cut(s) 702, 743, 801
BsiHKCI CYCGRG 3 cut(s) 71, 541, 548
BsiSI CCGG 2 cut(s) 549, 695
BslFI GGGAC 5 cut(s) 131, 164, 179, 565, 767
BslI CCNNNNNNNGG 5 cut(s) 71, 86, 548, 572, 830
BsmFI GGGAC 5 cut(s) 131, 164, 179, 565, 767
BsnI GGCC 1 cut(s) 135
BsoBI CYCGRG 3 cut(s) 71, 541, 548
Bsp120I GGGCCC 1 cut(s) 133
Bsp1286I GDGCHC 9 cut(s) 80, 137, 548, 563, 666, 702, 743, 801, 824
Bsp143I GATC 1 cut(s) 634
BspACI CCGC 2 cut(s) 59, 87
BspANI GGCC 1 cut(s) 135
BspLI GGNNCC 3 cut(s) 63, 77, 135
BspMAI CTGCAG 1 cut(s) 730
BspMI ACCTGC 2 cut(s) 92, 848
BspPI GGATC 1 cut(s) 642
BspT107I GGYRCC 1 cut(s) 75
BssECI CCNNGG 7 cut(s) 54, 129, 547, 548, 572, 640, 675
BssMI GATC 1 cut(s) 634
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 2 cut(s) 573, 831
Bst4CI ACNGT 3 cut(s) 145, 193, 558
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 628, 702
BstDEI CTNAG 2 cut(s) 114, 120
BstDSI CCRYGG 1 cut(s) 675
BstEII GGTNACC 1 cut(s) 668
BstF5I GGATG 3 cut(s) 261, 345, 387
BstKTI GATC 1 cut(s) 637
BstMBI GATC 1 cut(s) 634
BstMWI GCNNNNNNNGC 2 cut(s) 84, 115
BstNI CCWGG 2 cut(s) 573, 831
BstPI GGTNACC 1 cut(s) 668
BstSCI CCNGG 5 cut(s) 547, 548, 571, 693, 829
BstSFI CTRYAG 1 cut(s) 726
BstSLI GKGCMC 4 cut(s) 80, 137, 563, 743
BstV1I GCAGC 2 cut(s) 677, 712
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
BsuRI GGCC 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 675
BtsCI GGATG 3 cut(s) 261, 345, 387
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 7 cut(s) 219, 432, 474, 489, 516, 611, 735
BveI ACCTGC 2 cut(s) 92, 848
Cac8I GCNNGC 2 cut(s) 628, 702
Cfr13I GGNCC 2 cut(s) 133, 134
Cfr9I CCCGGG 1 cut(s) 548
CviAII CATG 1 cut(s) 40
DdeI CTNAG 2 cut(s) 114, 120
DpnI GATC 1 cut(s) 636
DpnII GATC 1 cut(s) 634
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 799
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 5 cut(s) 137, 548, 666, 801, 824
Eco53kI GAGCTC 1 cut(s) 799
Eco57I CTGAAG 1 cut(s) 635
Eco88I CYCGRG 3 cut(s) 71, 541, 548
Eco91I GGTNACC 1 cut(s) 668
EcoICRI GAGCTC 1 cut(s) 799
EcoO109I RGGNCCY 2 cut(s) 133, 134
EcoO65I GGTNACC 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 571, 829
EcoT14I CCWWGG 1 cut(s) 129
EcoT38I GRGCYC 5 cut(s) 137, 548, 666, 801, 824
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 1 cut(s) 43
FaiI YATR 1 cut(s) 41
FaqI GGGAC 5 cut(s) 131, 164, 179, 565, 767
FatI CATG 1 cut(s) 39
Fnu4HI GCNGC 4 cut(s) 60, 88, 691, 726
FokI GGATG 3 cut(s) 248, 332, 374
FriOI GRGCYC 5 cut(s) 137, 548, 666, 801, 824
Fsp4HI GCNGC 4 cut(s) 60, 88, 691, 726
FspBI CTAG 1 cut(s) 850
GluI GCNGC 4 cut(s) 60, 88, 691, 726
GsuI CTGGAG 1 cut(s) 465
HaeIII GGCC 1 cut(s) 135
HapII CCGG 2 cut(s) 549, 695
Hin1II CATG 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 5 cut(s) 5, 67, 430, 487, 517
HpaII CCGG 2 cut(s) 549, 695
HphI GGTGA 4 cut(s) 520, 680, 691, 829
Hpy166II GTNNAC 4 cut(s) 14, 655, 682, 741
Hpy188I TCNGA 2 cut(s) 66, 623
Hpy188III TCNNGA 1 cut(s) 794
Hpy8I GTNNAC 4 cut(s) 14, 655, 682, 741
Hpy99I CGWCG 4 cut(s) 173, 275, 317, 401
HpyCH4III ACNGT 3 cut(s) 145, 193, 558
HpyCH4IV ACGT 1 cut(s) 657
HpyCH4V TGCA 2 cut(s) 728, 741
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 115
HpyF3I CTNAG 2 cut(s) 114, 120
HpySE526I ACGT 1 cut(s) 657
Hsp92II CATG 1 cut(s) 43
Kzo9I GATC 1 cut(s) 634
LmnI GCTCC 3 cut(s) 67, 623, 804
Lsp1109I GCAGC 2 cut(s) 677, 712
MaeI CTAG 1 cut(s) 850
MaeII ACGT 1 cut(s) 657
MaeIII GTNAC 6 cut(s) 139, 187, 214, 454, 668, 805
MalI GATC 1 cut(s) 636
MboI GATC 1 cut(s) 634
MboII GAAGA 4 cut(s) 19, 22, 28, 769
MflI RGATCY 1 cut(s) 634
MhlI GDGCHC 9 cut(s) 80, 137, 548, 563, 666, 702, 743, 801, 824
MluCI AATT 1 cut(s) 327
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 612
MseI TTAA 2 cut(s) 330, 750
MslI CAYNNNNRTG 1 cut(s) 38
MspI CCGG 2 cut(s) 549, 695
MspR9I CCNGG 5 cut(s) 549, 550, 573, 695, 831
MvaI CCWGG 2 cut(s) 573, 831
MwoI GCNNNNNNNGC 2 cut(s) 84, 115
NciI CCSGG 3 cut(s) 549, 550, 695
NdeII GATC 1 cut(s) 634
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 3 cut(s) 63, 77, 135
NmeAIII GCCGAG 1 cut(s) 35
NmuCI GTSAC 5 cut(s) 187, 214, 454, 668, 805
PaqCI CACCTGC 1 cut(s) 848
PcsI WCGNNNNNNNCGW 3 cut(s) 62, 627, 672
PfeI GAWTC 4 cut(s) 67, 430, 487, 517
PflMI CCANNNNNTGG 1 cut(s) 572
PkrI GCNGC 4 cut(s) 61, 89, 692, 727
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 801
Psp6I CCWGG 2 cut(s) 571, 829
PspEI GGTNACC 1 cut(s) 668
PspGI CCWGG 2 cut(s) 571, 829
PspN4I GGNNCC 3 cut(s) 63, 77, 135
PspOMI GGGCCC 1 cut(s) 133
PspPI GGNCC 2 cut(s) 133, 134
PstI CTGCAG 1 cut(s) 730
PsuI RGATCY 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 38
SacI GAGCTC 1 cut(s) 801
SaqAI TTAA 2 cut(s) 330, 750
SatI GCNGC 4 cut(s) 60, 88, 691, 726
Sau3AI GATC 1 cut(s) 634
Sau96I GGNCC 2 cut(s) 133, 134
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 5 cut(s) 549, 550, 573, 695, 831
SduI GDGCHC 9 cut(s) 80, 137, 548, 563, 666, 702, 743, 801, 824
SfcI CTRYAG 1 cut(s) 726
SmaI CCCGGG 1 cut(s) 550
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 755, 794
SmoI CTYRAG 2 cut(s) 755, 794
Sse9I AATT 1 cut(s) 327
SsiI CCGC 2 cut(s) 59, 87
SspMI CTAG 1 cut(s) 850
SstI GAGCTC 1 cut(s) 801
StyD4I CCNGG 5 cut(s) 547, 548, 571, 693, 829
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 3 cut(s) 145, 193, 558
TaiI ACGT 1 cut(s) 660
TasI AATT 1 cut(s) 327
TauI GCSGC 2 cut(s) 62, 90
TfiI GAWTC 4 cut(s) 67, 430, 487, 517
Tru1I TTAA 2 cut(s) 330, 750
Tru9I TTAA 2 cut(s) 330, 750
TscAI CASTG 7 cut(s) 219, 432, 474, 489, 516, 618, 735
TseFI GTSAC 5 cut(s) 187, 214, 454, 668, 805
TseI GCWGC 2 cut(s) 690, 725
Tsp45I GTSAC 5 cut(s) 187, 214, 454, 668, 805
TspDTI ATGAA 2 cut(s) 22, 28
TspMI CCCGGG 1 cut(s) 548
TspRI CASTG 7 cut(s) 219, 432, 474, 489, 516, 618, 735
Van91I CCANNNNNTGG 1 cut(s) 572
VneI GTGCAC 1 cut(s) 739
XmaI CCCGGG 1 cut(s) 548
XspI CTAG 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.